Functional Analysis of the Plant Vacuolar Proton-Pumping Pyrophosphatase

Lead Research Organisation: John Innes Centre
Department Name: Contracts Office

Abstract

Plant vacuolar membranes are unusual in containing two classes of proton pump: an ATPase (V-ATPase) and an inorganic pyrophosphatase (V-PPase). While the V-ATPase is ubiquitous among eukaryotes, the V-PPase is restricted in its distribution to plants, some photosynthetic bacteria and a few members of the Archaea. Vacuolar proton pumps are known to acidify the vacuolar lumen, thereby generating the protonmotive force (PMF) required for proton-coupled transport of solutes into the vacuole via antiporters.
In Arabidopsis, a family of three AVP genes encodes V-PPases. We have shown in avp1 mutants that PPi-driven proton currents are completely absent at the vacuoles of many cell types. [AVP2 is known to be Golgi-localised, while expression of AVP3 is limited to pollen.] The phenotype of avp1 mutants is subtle under nutritionally-replete conditions. However, mutants are hypersensitive to salinity. Surprisingly, this sensitivity relates to chloride ions rather than to sodium. In accord with the notion that a V-PPase-generated PMF might play a critical role in anion accumulation in vacuoles, avp1 mutants are also exhibit a marked phenotype if nitrogen is supplied as nitrate rather than ammonium.
Future work will address the mechanistic basis of the role of the V-PPase in anion nutrition through measurement of vacuolar pH in vivo with ion-selective electrodes.

Publications

10 25 50
 
Description The plant cell vacuole has two proton pumps that are important for energizing the transport systems. The specific role of one of these pumps, the proton-pumping pyrophosphatase in vacuolar function is being characterised.
Exploitation Route Many commercailly important processes in plants depend on the function of the vacuole. For example, when supplied in excess nutrients can be stored in the vacuole and this process has important consequences for fertiliser use efficiency.
Sectors Agriculture, Food and Drink,Environment

 
Description The role of this important vacuole pump in crop nutrient use efficiency has been shown.
First Year Of Impact 2010
Sector Agriculture, Food and Drink,Environment
Impact Types Societal,Economic

 
Description CEPAMS Symposium Shanghai 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact CEPAMS Symposium Shanghai 2016 Friday 18th March
Editing Long Distance Transport and Storage to Optimize the Site of Nitrogen Assimilation in Drought and Salt-stress
Year(s) Of Engagement Activity 2016
 
Description International Symposium on Plant Membrane Biology, Annapolis MD, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact International Symposium on Plant Membrane Biology, Annapolis MD, 2016
Thursday 9th June 2016 S16 Future Perspectives
Year(s) Of Engagement Activity 2016
 
Description Invited Seminar at Bristol University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited seminar speaker at Bristol University - title 'The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?'
Year(s) Of Engagement Activity 2017
 
Description Invited Seminar at SIPPE Shanghai Institute of Plant Physiology and Ecology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact 4th November 2016 in Shanghai 15:00
Invited Seminar at SIPPE Shanghai Institute of Plant Physiology and Ecology
Year(s) Of Engagement Activity 2016
 
Description Invited seminar speaker Padua Italy (12th January 2016) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation on the role of the plant lytic vacuole.
Title: Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2016
 
Description Invited seminar speaker Plant Sciences Univiersity of Oxford, UK (January 28 th 2016) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited Departmental seminar speaker. Topic was the role of the lytic vacuole.
Title: The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2016
 
Description Invited speaker at SEB Plant Transport Group Meeting Glasgow 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited presentation on the functional role of the vacuole proton pumping pyrophosphatase.
Year(s) Of Engagement Activity 2014
 
Description Invited speaker at York University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited speaker to York University: Plant Biology Seminar - The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2017
 
Description Member of the UK Science Partnership for Animal and Plant Health 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Member of the UK Science Partnership for Animal and Plant Health - quarterly meetings are held
Year(s) Of Engagement Activity 2017
 
Description Opening address at Breeders Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Opening address at Breeders Day at the John Innes Centre
Year(s) Of Engagement Activity 2017
 
Description Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain
Year(s) Of Engagement Activity 2017
 
Description Salt & Water Stress in Plants - Gordon conference presentation Hong Kong 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Report research to the scientific community
Year(s) Of Engagement Activity 2012
URL http://www.grc.org/programs.aspx?year=2012&program=salt
 
Description Scientific Advisory Board Member for Max PIanck Institute of Molecular Plant Physiology, Golm, Germany 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Scientific Advisory Board Meeting for Max PIanck Institute of Molecular Plant Physiology
Year(s) Of Engagement Activity 2017
 
Description World Life Science Conference (Sustainable Agriculture Session), Beijing, 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact World Life Science Conference (Sustainable Agriculture Session), Beijing, 2016
3rd November 2016, Session 48 Sustainable Agriculture - Membrane Transporters and Nutrition - from crops to people
Year(s) Of Engagement Activity 2016