Cloning wheat rust resistance genes by sequence capture on structured populations

Lead Research Organisation: John Innes Centre
Department Name: Contracts Office

Abstract

Rust resistance genes in cereal crops are often overcome within a few seasons when deployed one at a time. Introgressing multiple resistance genes into a cultivar is one strategy that may contribute to more durable resistance; however, when this process is done by traditional breeding methods it involves long breeding trajectories and can result in linkage drag due to deleterious alleles. We are pursuing a transgenic approach to control wheat stem rust. Our strategy is to isolate multiple novel stem rust resistance (Sr) genes and deploy them in combination at a single transgene locus in wheat. This strategy should avoid linkage drag and ensures that the genes stay together, thus avoiding single genes again being exposed to the pathogen. This project will support our ongoing efforts to combine targeted sequence capture, high throughput sequencing and comparative genomics on genetically structured populations to speed up the cloning of Sr genes from diploid relatives of wheat and from wheat alien introgression lines.
 
Title 2nd Prize in Engaging Images Art Competition, The Sainsbury Centre for Visual Arts 
Description Josh Waites' artwork based on the MARPLE wheat rust diagnostic tool from the @Saunders_Lab came second in the Engaging Images Art Competition. Due to MARPLE's use in Ethiopia, he painted it in a traditional orthodox Ethiopian art style. It was displayed at The Sainsbury Centre for Visual Arts during the month of December 2019. 
Type Of Art Artwork 
Year Produced 2019 
Impact Not aware of any. 
URL https://twitter.com/JoshOWaites/status/1203311578571059200
 
Title Blog on "Wild wheat, plant genomics, and food security" 
Description I wrote a blog for the Global Engage conference "Plant Genomics and Gene Editing Congress: Europe". 
Type Of Art Creative Writing 
Year Produced 2020 
Impact The blog was well received and widely tweeted. 
URL http://www.global-engage.com/agricultural-biotechnology/wild-wheat-plant-genomics-and-food-security/
 
Title Wrestling genes out of the giant barley and wheat genomes 
Description Blog for general science audience on how to rapidly clone genes from wheat or barley 
Type Of Art Creative Writing 
Year Produced 2016 
Impact Postive feedback from friends and colleagues. 
URL http://blogs.biomedcentral.com/on-biology/2016/10/24/wrestling-genes-out-of-the-giant-barley-and-whe...
 
Description This grant helped to refine MutRenSeq, a technique for accelerating identification (molecular cloning) of disease resistance genes in wheat. In the past, cloning resistance genes in wheat typically took 5-10 years. With MutRenSeq this can now be done in just 2 years.

Note added: 08 March 2018: This technology, and others which we have developed, is fuelling an exciting revolution in resistance gene cloning in Triticeae, in particular wheat, wheat wild relatives, barley, and wild barley. This will likely provide the material needed to engineer multi resistance gene stacks, which, providing the socio-political impasse on GM crops, and in particular GM wheat, could be overcome, could lead to a revolution in resistance gene deployment to achieve more durable resistance and less dependence on pesticides.
Exploitation Route There are ongoing discussions between the 2Blades Foundation, USA (a co-founder of my research), CIMMYT (a public good breeding institute serving the developing world), and the CSIRO-Australia, for engineering a multi-transgene stack containing multiple broad-spectrum stem rust resistance genes for more durable resistance to this devastating disease of wheat.
Sectors Agriculture, Food and Drink

 
Description The findings helped support a GM field trial for stem rust resistance in Minnesota, USA. See: http://www.global-engage.com/agricultural-biotechnology/wild-wheat-plant-genomics-and-food-security/ http://2blades.org/2019/03/12/wheat-lines-from-2blades-csiro-and-umn-exhibit-exceptional-stem-rust-resistance-in-the-field/
First Year Of Impact 2019
Sector Agriculture, Food and Drink,Environment
Impact Types Societal,Policy & public services

 
Description 2Blades Foundation
Amount $20,000 (USD)
Organisation 2Blades Foundation 
Sector Charity/Non Profit
Country United States
Start 07/2017 
End 07/2018
 
Description A multi-R gene stack for durable resistance to wheat stem rust (with 2Blades Foundation)
Amount £939,147 (GBP)
Organisation Two Blades Foundation 
Sector Charity/Non Profit
Country United States
Start 07/2014 
End 06/2017
 
Description BBSRC "Impact Acceleration Account"
Amount £13,078 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 02/2018
 
Description BBSRC - ISCF Agri-food Technology Catalyst
Amount £29,943 (GBP)
Funding ID BB/SCA/JIC/17 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2017 
End 02/2018
 
Description BBSRC NRP-DTP iCASE
Amount £113,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description BBSRC iCASE
Amount £113,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description Cloning and characterization of Sr43 and stem rust resistance genes SrA, SrB, SrC and SrD from Aegilops sharonensis
Amount £100,165 (GBP)
Organisation Two Blades Foundation 
Sector Charity/Non Profit
Country United States
Start 07/2019 
End 10/2020
 
Description Doctoral Trainingship Programme
Amount £97,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 09/2021
 
Description FSOV
Amount € 255,538 (EUR)
Organisation French Wheat Research Fund (FSOV) 
Sector Charity/Non Profit
Country France
Start 02/2017 
End 01/2020
 
Description FSOV
Amount € 279,216 (EUR)
Organisation French Wheat Research Fund (FSOV) 
Sector Charity/Non Profit
Country France
Start 02/2017 
End 01/2020
 
Description GCRF-IAA funding: Wheat blast resistance partnership development with Bangladesh
Amount £17,486 (GBP)
Funding ID BB/GCRF-IAA/17/11 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2016 
End 03/2017
 
Description Global Challenges Research Fund
Amount £316,622 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 07/2018
 
Description Global Challenges Research Fund - Data and Resources
Amount £897,694 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2016 
End 07/2017
 
Description John Innes Centre KEC Innovation Fund
Amount £40,499 (GBP)
Organisation John Innes Centre 
Sector Academic/University
Country United Kingdom
Start 08/2015 
End 12/2016
 
Description Marie Sklodowska Curie Action International Training Network
Amount € 238,507 (EUR)
Organisation Marie Sklodowska-Curie Actions 
Department Initial Training Networks (ITN)
Sector Charity/Non Profit
Country Global
Start 01/2016 
End 12/2019
 
Description Monsanto Beachel-Borlaug International Scholarship
Amount $220,000 (USD)
Funding ID MBBIS 06-400258-12580 
Organisation Monsanto 
Sector Private
Country United States
Start 07/2016 
End 06/2020
 
Description Newton Institutional Links Grant
Amount £299,973 (GBP)
Organisation Newton Fund 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2020
 
Description Norwich Research Park Translational Fund
Amount £41,272 (GBP)
Organisation Norwich Research Park 
Sector Private
Country United Kingdom
Start 11/2016 
End 06/2017
 
Description Safeguarding our daily bread from wheat rust diseases
Amount £2,930 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2019 
End 08/2019
 
Description Understanding and exploiting genetic diversity in wheat for yellow rust resistance
Amount £113,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2020 
End 12/2023
 
Title Open Wild Wheat raw sequences 
Description We provide access to whole genome shotgun sequences of a genetically diverse panel of 151 accessions of Aegilops tauschii generated under the aegis of the Open Wild Wheat Consortium (www.openwildwheat.org). 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact We have used the data to implement a 'shotgun' genome-wide association study (GWAS) pipeline for rapid gene discovery and identificaiton. Based on this, we have identified candidate genes for stem rust, wheat blast and trichome density. We have had very many requests from the community to implement this technology, and we expect it will fuel an exponential rise in cloned genes within the Triticeae. 
URL https://opendata.earlham.ac.uk/wheat/under_license/toronto/Wulff_2018-01-31_OWWC/
 
Description Collaboration with Jonathan Jones funded by Betty and Gordon Moore Foundation via 2Blades Foundation 
Organisation The Sainsbury Laboratory
Country United Kingdom 
Sector Academic/University 
PI Contribution We have used RenSeq to genotype 200 Watkins wheat landrace lines.
Collaborator Contribution My colleague Jonathan Jones kindly offered to collaborate on the sequence-configuration of our Watkins wheat landrace panel by paying for RenSeq genotyping of 100 lines.
Impact This collaboration has allowed us to increase the size of our sequence configured panel from 200 to 300. This will improve the resolution wich which we can discover and clone resistance genes in the panel.
Start Year 2018
 
Title Wheat stem rust resistance genes and methods of use 
Description The patent describes the cloning and characterisation of two stem rust resistance genes in wheat. 
IP Reference WO2017024053 
Protection Patent application published
Year Protection Granted 2015
Licensed No
Impact The genes have been added to multi-R gene stacks and transformed into wheat and barley. These will be field trialled in Minnesota, USA in the 2018 growing season.
 
Title AgRenSeq - GitHub 
Description AgRenSeq is a pipeline to identify candidate resistance (R) genes in plants directly from a diversity panel. The diversity panel needs to be sequenced (R gene enrichment sequencing - RenSeq) and phenotyped. Phenotype scores need to be converted to AgRenSeq scores that assign positive values to resistance and negative values to suscetibility. An intermediate phenotype should have an AgRenSeq score close to zero. For RenSeq you will need a bait library that targets R genes in your plant species. A bait library for Aegilops tauschii can be found here. We reccomend Arbor biosciences for synthesis of baits. They also offer the enrichment service. 
Type Of Technology Software 
Year Produced 2018 
Impact Requests for collaboration from academia and industry. 
 
Title MutRenSeq Mutant Hunter 
Description The MutRenSeq Mutant Hunter is a pipeline to combine resistance gene enrichment sequencing (RenSeq) with EMS mutant screens to identify NLR-type resistance genes. 
Type Of Technology Software 
Year Produced 2015 
Open Source License? Yes  
Impact This pipeline has resulted in collaborations (some on responsive mode grants), research agreements, and further funding (e.g. from the 2Blades Foundation, USA). 
URL https://github.com/steuernb/MutantHunter/
 
Description 5th Plant Genomics Workshop, Amsterdam 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented my work on rapid gene cloning and speed breeding at the 5th Plant Genomics Workshop, Amsterdam. This lead to questions and discussion with other scientists and representatives from industry, and I was invited back to present again in 2018, as well as invited to the advisory board.
Year(s) Of Engagement Activity 2017
 
Description Annual Science Meeting 2018, JIC and TSL 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I was called upon to give an update of my work at the JIC and TSL Annual Science Meeting 2018.
Year(s) Of Engagement Activity 2018
 
Description BMGF Grand Challenges Meeting, Berlin, Germany 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to the BMGF Grand Challenges Meeting, in Berlin, Germany. This has since led to further interactions with the BMGF and DFID funded DGGW project, and a request for a workshop at the next DGGW meeting which will be held in the John Innes Centre in 2020.
Year(s) Of Engagement Activity 2019
 
Description CerealPath PhD Course on Scientific Soft Skills 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I co-organised and taught a course on scientific soft skills for a group of PhD students from the EU funded CerealPath programme.
Year(s) Of Engagement Activity 2016
URL https://cerealpath.eu/
 
Description Cranfield University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact I was called upon to give a lecture to MSc students on the Cranfield University bioinformatics course. I was asked to come back again in May for a two day visit to teach scientific soft skills for early career scientists.
Year(s) Of Engagement Activity 2019
 
Description DivSeek Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was called upon at short notice to give a presentation at the DivSeek workshop on how to exploit germplasm collections for rapid trait identificaiton and deployment.
Year(s) Of Engagement Activity 2019
 
Description Food Ingreedients: "Speed cloning: Transforming crop disease protection with higher yielding, resilient "elite crops"" 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was interviewed by the online magazine "Food Ingredients" about protecting crops with cloned resistance genes.
Year(s) Of Engagement Activity 2019
URL https://www.foodingredientsfirst.com/news/speed-cloning-transforming-crop-disease-protection-with-hi...
 
Description Group member Postdoc Kumar Gaurav invited to present Keynote Lecture at EuroBiotech, Kraków, Poland, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote Lecture, EuroBiotech, Kraków, Poland, 2019
Year(s) Of Engagement Activity 2020
URL https://www.eurobiotech.krakow.pl/gb/program-x/program.html
 
Description Interview by Deutchlandfunk (German radio station) 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact In the wake of my Nature Biotechnology paper on rapid resistance gene cloning by associaiton genetics, I was interviewed by Deutchlandfunk, a german radio station equivalent to Radio BBC 4 in the UK.
Year(s) Of Engagement Activity 2019
URL https://www.deutschlandfunk.de/resistenzgene-bei-pflanzen-hilfe-von-der-starken.676.de.html?dram:art...
 
Description Interview by Jerome Starkey, The Times 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Following our press release on the appearance of stem rust in the UK for the first time in 50 years, I was interviewed by The Times correspondent Jereme Starkey. This resulted in a short article in The Times.
Year(s) Of Engagement Activity 2018
URL https://www.thetimes.co.uk/article/plague-that-scared-the-romans-is-back-g6nzm5d0k
 
Description Interview with Newsweek on genetic diversity 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact I was interviewed by Newsweek on the importance of genetic diversity in crops.
Year(s) Of Engagement Activity 2018
URL http://www.newsweek.com/feed-world-scientists-have-plan-save-chickpeas-destruction-806925
 
Description Interviews with press and media on speed breeding 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact JIC External Relations department released a press release on our work on 'speed breeding'. This sparked considerable attention from the press and media. I expect I gave about 20 interviews in the ensuing days, featured on BBC Look East, the local press, two national newspapers (The Times and The Guardian), Newsweek, as well as countless other media outlets across the world, including in Japanese, Chinese and Arabic.
Year(s) Of Engagement Activity 2018
 
Description Invitation to present work at Wiersum Plantbreeding, The Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to visit Wiersum Plantbreeding in the Netherlands. We discussed potential future collaborative work around the concept of a resistance gene atlas for wheat.
Year(s) Of Engagement Activity 2019
 
Description Invited presentaiton (plenary session) at the Molecular Plant Microbe Interactions congress in Glasgow 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation of my work at the 2019 MPMI meeting in Glasgow. Based on this, I have since received two additional invitations to give seminars in Oxford University and Edinburgh University.
Year(s) Of Engagement Activity 2019
URL https://www.ismpmi.org/Congress/Pages/default.aspx
 
Description Invited presentaiton at G20 Wheat Meeting in Frankfurt 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation at the G20 wheat meeting in Frankfurt. The presentation sparked questions and led to requests for futher information and collaboration.
Year(s) Of Engagement Activity 2016
URL https://www.bmel.de/SharedDocs/TermineVeranstaltungen/EN/16-12-08-InternationalWheatCongress.html
 
Description Invited presentation at 7th Central European Congress of Life Sciences Eurobiotech, Krakow, Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact My postdoc Kumar Gaurav was invited to give a Keynote Lecture at the the 7th Central European Congress of Life Sciences Eurobiotech, Krakow, Poland.
Year(s) Of Engagement Activity 2019
URL https://www.eurobiotech.krakow.pl/gb/
 
Description Invited presentation at AgriTech East, NIAB, Cambridge, UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact AgriTech East dissemination activity to local farmers, breeders and industry stake holders on rust diseases of wheat.
Year(s) Of Engagement Activity 2016
URL https://www.cambridgenetwork.co.uk/events/pollinator-rusts-and-other-cereal-killers/
 
Description Invited presentation at BASF, Belgium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to BASF in Ghent, Belgium to present my work on rapid gene discovery and cloning in crops. We later discussed potential collaborations. This has resulted in further requests for information and discussions on collaboration.
Year(s) Of Engagement Activity 2020
 
Description Invited presentation at BeCA-ILRI (Nairobi, Kenya) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact This presentation sparked questions and discussions.

Expansion of my scientific network and opportunities for future collaborations.
Year(s) Of Engagement Activity 2014
 
Description Invited presentation at Elsoms Ltd, Spalding 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact I was invited to give a presentation to the vegatble and herb breeding company Elsoms Ltd in Spalding, Lincolnshire. The presentation lead to request for further information and
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at IPK, Gatersleben, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I gave a presentation on engineering resistance to the stem rust fungus in wheat using cloned resistance genes from wild grasses.
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at Institute of Experimental Botany, Czech Republic 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to the Institute of Experimental Botany in the Czech Republic to give a presentation about my work on rapid gene discovery and cloning in Triticeae.
Year(s) Of Engagement Activity 2018
 
Description Invited presentation at Julius Kühn Institute, Quedlinburg, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Policymakers/politicians
Results and Impact I gave a presentation on engineering resistance to the stem rust fungus in wheat using cloned resistance genes from wild grasses.
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at KAUST, Thuwal, Saudi Arabia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I visited KAUST to present my work. This has resulted in offers of further collaboration.
Year(s) Of Engagement Activity 2020
 
Description Invited presentation at Kew 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was invited to give a short seminar on how to exploit genetic variation in crop wild relatives at a joint workshop organised between the John Innes Centre and Kew.
Year(s) Of Engagement Activity 2018
 
Description Invited presentation at Key's Symposium, Keygene, Netherlands 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to give a presentation on my work at Keygene, The Netherlands. This resulted in requests for further information and interactions.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at National Institute of Botany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I gave a presentation on next generation disease resistance breeding in wheat.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation at Plant Sciences Department, University of Oxford 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact I was invited to present my work at the Plant Sciences Department at the University of Oxford.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at Plant and Animal Genome Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact My postdoc, Kumar Gaurav, was invited to present our work on rapid gene cloning at PAG. This resulted in requests for further collaboration from groups in USA.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at Pwani Univeristy, Kenya 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact As part AfriPlantSci, a two-week course on plant sciences for African scientists, I was invited to give a presentation at Pwani University, Kenya describing my research on rapid gene cloning and speed breeding in cereals.
Year(s) Of Engagement Activity 2019
URL https://acaciaafrica.org/afriplantsci/
 
Description Invited presentation at Technical University of Munich 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation at the TUM on my speed breeding and rapid gene cloning work in cereals.
Year(s) Of Engagement Activity 2019
URL https://portal.mytum.de/termine/Event.2019-03-20.1464449129/event_view?
 
Description Invited presentation at The Sainsbury Laboratory 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work in The Sainsbury Laboratory spring science meeting.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at The Sainsbury Laboratory, Norwich, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Was invited to give a presentation of my work on wheat rusts at the annual Sainsbury Lab retreat.
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at University College Dublin 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I gave a presentaiton to undergraduates and research scientists on the use of mutational genomics to isolate disease resistance genes.
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at University of Nottingham 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I was invited to give a presentation on my work at the University of Nottingham to share my latest results on rapid resistance gene discovery, cloning and deployment. This resulted in long discussions.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at the 8th Plant Genomics & Gene Editing Congress, Rotterdam, The Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on rapid gene discovery and cloning, and generation advancement at the 8th Plant Genomics & Gene Editing Congress, Rotterdam, The Netherlands.
Year(s) Of Engagement Activity 2020
URL http://www.global-engage.com/event/plant-genomics/
 
Description Invited presentation at the Australian National University, Canberra, Australia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I gave a presentation on cloning and stacking stem rust resistance genes in wheat.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation at the Bill and Melinda Gates Foundation Grand Challenges Meeting in London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was asked to give a presentation on molecular breeding for durable disease resistance, including my work on speed breeding and speed cloning. The presentation sparked considerable interest and I was asked to chair two round table discussions on the topic where I answered many questions and was requested additional information.
Year(s) Of Engagement Activity 2016
URL http://grandchallenges.org/videos
 
Description Invited presentation at the Institute of Cereal Crop Research, Tel Aviv, Israel 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Gave a presentation on the use of genetic diversity from wild grasses to improve wheat disease resistance
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at the Ludwig Maximilian University of Munich 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was invited to give a presentation at the LMU about my work on rapid gene isolation and speed breeding in cereals.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at the Molecular Plant Microbes Interaction Meeting in Portland, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation on my work on speed breeding and wheat rusts at the biennial congress on Molecular Plant-Microbe Interactions.
Year(s) Of Engagement Activity 2016
URL http://www.ismpmi.org/Congress/2016/MeetingSchedule/Pages/default.aspx
 
Description Invited presentation at the University of Copenhagen 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a presentation on the cloning of stem rust resistance genes from wheat and wild relatives and their potential use in transgenic wheat.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation at the Wheat Durable Resistance Meeting, Minneapolis, USA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation at the Wheat Durable Resistance Meeting in Minneapolis, USA. The presentation lead to debate and request for additional information and collaboration.
Year(s) Of Engagement Activity 2016
URL https://www.ag.ndsu.edu/wheatresistance
 
Description Invited presentation at the Wheat Initiative Expert Working Group, Minneapolis, USA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to join the wheat Expert Working Group on Control of Wheat Pathogens and Pests and deliver a presentation on available genomics and germplasm resources and what the community needs in this respect. The presentation lead to debate and requests for germplasm sharing with other labs.
Year(s) Of Engagement Activity 2016
URL http://www.wheatinitiative.org/activities/expert-working-groups/control-wheat-pathogens-and-pests
 
Description Invited presentation at the wheat breeding company KWS 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I visited the wheat breeding company KWS at their Cambridge site and gave a presentation on how to use mutational genomics approaches to identify markers that can be used in breeding. The presentation sparked interest and generated significant debate.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation by lab member PhD student Sreya Ghosh at Plant and Animal Genome Conference, 2020. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact My PhD student Sreya Ghosh was invited to present at PAG, 2020 in San Diego. This resulted in request for additional information and has led to the drafting of a patent application with the 2Blades Foundation.
Year(s) Of Engagement Activity 2020
 
Description JIC press release on AgRenSeq: "Rapid gene cloning technique will transform crop disease protection" 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The press release was picked up by the national and international media and led to >10 requests for interviews from the press and media, including BBC Radio 4, The Times, Deutchlandfunk and others.
Year(s) Of Engagement Activity 2019
URL https://www.jic.ac.uk/press-release/rapid-gene-cloning-technique-will-transform-crop-disease-protect...
 
Description Keynote Speaker, Advances in Integrated Pest Management, Association of Applied Biologists, Harper Adams University, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was invited to be a Keynote Speaker, at the meeting entitled Advances in Integrated Pest Management, Association of Applied Biologists, Harper Adams University, UK. This led to futher request to be involved in a responsive mode GCRF collaboration, which regretfully was not funded.
Year(s) Of Engagement Activity 2017
 
Description Keynote Speaker, German Plant Breeding Society, Wernigerode, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was the Keynote Speaker, German Plant Breeding Society, Wernigerode, Germany. This resulted in additional interest from a range of colleagues in academia and industry, including policy makers.
Year(s) Of Engagement Activity 2018
 
Description Laboratory News: "Fast Food" 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was interviewed by the online and print magazine Laboratory News about my new rapid gene cloning technology.
Year(s) Of Engagement Activity 2019
URL https://www.popsci.com/crops-disease-resistant-genes
 
Description Newspaper article in the Times: "Gene analysis speeds up race to beat cereal killer" 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact I was intereviewed by a reporter from The Times on our breakthrough in resistance gene cloning based on phenotyping sequence-configured diversity panels.
Year(s) Of Engagement Activity 2019
URL https://www.thetimes.co.uk/article/gene-analysis-speeds-up-race-to-beat-cereal-killer-j2vsht83v
 
Description Plant Genomics and Gene Editing Congress, Rotterdam, The Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to present at a Congress in Rotterdam.
Year(s) Of Engagement Activity 2018
 
Description Plenary Speaker, 4th International Conference on Plant Genetics, Genomics, Bioinformatics and Biotechnology, Almaty, Kazakhstan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on speed cloning and speed breeding as a Plenary Speaker at the 4th International Conference on Plant Genetics, Genomics, Bioinformatics and Biotechnology, Almaty, Kazakhstan. This led to stimulating discussions and contributed to requests for further involvment.
Year(s) Of Engagement Activity 2017
 
Description Popular Science: "These genetic 'goggles' could help us engineer wildly resilient crops" 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was interviewed by the online magazine Popular Science about my work on rapid resistance gene cloning.
Year(s) Of Engagement Activity 2019
URL https://www.popsci.com/crops-disease-resistant-genes
 
Description Postdoc member Kumar Gaurav invited to present at Monogram Workshop, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited presentation at the Monogram Workshop, 2019.
Year(s) Of Engagement Activity 2020
 
Description Presentation at 4th International Conference "Plant Biotic Stresses & Resistance Mechanisms", Vienna, Austria 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I presented my work at the 4th International Conference "Plant Biotic Stresses & Resistance Mechanisms", Vienna, Austria.
Year(s) Of Engagement Activity 2020
URL http://viscea.org/plant-biotic-stresses-resistance-mechanisms-iv-february-19-20-2020/
 
Description Presentation at Institute of Genetics and Developmental Biology, Beijing, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to visit the Institute of Genetics and Developmental Biology, Beijing, China and present my work on speed cloning and speed breeding. This has led to follow up requests for further visits, collaboration, and applicaiton for joint funding opportunities.
Year(s) Of Engagement Activity 2017
 
Description Presentation at International Wheat Conference, Saskatoon, Canada 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact MINING A SEQUENCE-CONFIGURED WILD WHEAT DIVERSITY PANEL FOR GENES REGULATING ENVIRONMENTAL RESPONSE USING ASSOCIATION GENETICS
Year(s) Of Engagement Activity 2019
 
Description Presentation at John Innes Centre Wheat Breeders Day 2019 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact We presented a talk on "Mining wild wheat diversity for disease resistance". This sparked questions and discussions. I have been asked to visit BASF in Gent, Belgium to discuss potential collaboration.
Year(s) Of Engagement Activity 2019
 
Description Presentation at Nanjing Agricultural University, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to Nanjing Agricultural University, China where I gave a presentation on my work on speed cloning and speed breeding. I was invited to participate in further collaborations based on this visit.
Year(s) Of Engagement Activity 2017
 
Description Presentation at Science Innovation Showcase, JIC, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to present at the Science Innovation Showcase, JIC, UK. This has led to requests for further information and ongoing disussions with inustrial partners for collaboration / contract research.
Year(s) Of Engagement Activity 2018
 
Description Presentation at Tel Aviv University, Israel 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on speed breeding and speed cloning at Tel Aviv University, Israel. This led to further discussions and resulted in an active collaboration and exchange of germplasm.
Year(s) Of Engagement Activity 2017
 
Description Presentation to Appolinaire Dijkeng and Jacob Mignouna, BecA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I gave a presentation to Appolinaire Dijkeng and Jacob Mignouna from the BecA-ILRI Hub in Nairobi, Kenya. This was part of ongoing discussions and collaborative work between JIC and BecA-ILRI.
Year(s) Of Engagement Activity 2017
 
Description Presentation to Elsoms Seeds, Spalding, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on speed breeding and resistance gene cloning to Elsoms Seeds, Lincolnshire, and to troubleshoot their LED lighting in the glasshouse. This has since resulted in further interactions and dicussions.
Year(s) Of Engagement Activity 2017
 
Description Presentation to Joe Cerrell & James Birch, BMGF 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Supporters
Results and Impact Joe Cerrell & James Birch frin the Bill and Melinda Gates Foundation came to visit JIC and I was called upon to present my work on wheat stem rust and the solution we are working on to try to mitigate this problem for small holder farmers in Africa.
Year(s) Of Engagement Activity 2016
 
Description Presentation to KWS, Einbech, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to present my work on speed cloning and speed breeding at KWS, Einbech, Germany. KWS are currently considering how our enabling technologies could be integrated in one of their own crop breeding programmes and how we may collaborate to achieve this.
Year(s) Of Engagement Activity 2018
 
Description Presentation to delegation from Elsoms Seeds, Lincolnshire, UK 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact I invited 8 delegates from Elsoms Seeds to visit JIC for some bespoke presentations from my group and a colleauge, and for an inspection of our speed breeding facilities. This has helped cement a relationship that now stretches back more than one year.
Year(s) Of Engagement Activity 2018
 
Description Science Collaboration Agritech, Aquaculture and Food Safety Forum, Muscat, Sultanate of Oman 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present at the Science Collaboration Agritech, Aquaculture and Food Safety Forum, Muscat, Sultanate of Oman.
Year(s) Of Engagement Activity 2018
 
Description Short presentation to Eric Danquah, WACCI, Ghana 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was called upon to give a presentation to a visiting dignitary, Eric Danquah from the WACCI, Ghana. This lead to a lively discussion and exhange of ideas and request for further collaboration.
Year(s) Of Engagement Activity 2016
 
Description Teleconference discussion with Syngenta 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Participated in a teleconference with Syngenta with participation from China, UK and USA. Follow-on discussions are ongoing.
Year(s) Of Engagement Activity 2018
 
Description The Sainsbury Laboratory Annual Retreat 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I presented my yearly update to The Sainsbury Laboratory as part of my Adjunct Affiliation with this institution.
Year(s) Of Engagement Activity 2018
 
Description The Sainsbury Laboratory Retreat 2017, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on R gene cloning and speed breeding at The Sainsbury Laboratory 2017 retreat. This sparked questions and discussions and has since contributed to further collaboration with colleagues in TSL.
Year(s) Of Engagement Activity 2017
 
Description Two invited presentations at Next-generation wheat breeders workshop, University of Saskatchewan, Canada 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Myself and my postdoc, Sanu Arora, were invited to present on our enabling technology for rapid cloning of disease resistance genes by association genetics. This sparked a lot of interest and led to an invitation to present again at the International Wheat Congress, Saskatchewan, Canada, the following week.
Year(s) Of Engagement Activity 2019
URL https://2019iwc.ca/pre-conference-workshop/
 
Description Wheat molecular genomics workshop in Cairo, Egypt 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact In collaboration with Dr Ahmed Elkot of the Agricultural Research Centre (ARC) in Cairo, Egypt, we organised a workshop for wheat researchers and breeders. Approximately 50 scientists attended including the Minister of Agriculture and the President of the ARC (which employs ~60,000 staff). The event was a great success and covered in the national media in Egypt. The ARC staff were very honoured that the Minister of Agriculture attended.
Year(s) Of Engagement Activity 2019
URL https://www.facebook.com/526865257491839/posts/1170288249816200/
 
Description Workshop at BecA-ILRI, Kenya on Molecular Breeding in Small Grain Cereals 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I co-organised and taught a short 4-day course on molecular breeding in small grain cereals (wheat, barley and teff) for African scientist and breeders. Participants came from Ethiopia (~12), Kenya, Uganda, Tanzania, Madagascar, Nigeria and Burundi. The course was well received, led to requests for additional information, request to repeat the course the following year, and the possibility of a new strategically interesting collaboration with young career wheat scientist from Ethiopia.
Year(s) Of Engagement Activity 2017
URL http://hpc.ilri.cgiar.org/beca/training/jic_beca_2016/index.html
 
Description Workshop on Scientific Softskills, Punjab Agricultural University, Ludhiana, India 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I organised and presented, with colleagues, a workshop on scientific soft skills to early career scientists primarily from India, but also from the UK, Tunisia and Iran.
Year(s) Of Engagement Activity 2017
 
Description Workshop on scientific communication for early career scientists in Pwani, Kenya 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Along with my colleague Prof. Cristobal Uauy, I was invited to give a two-day course on scientifict communication to early career scientists from across Sub Saharan Africa as part of the ACACIA AfriPlantSciences course. The course involved practical sessions on scientific writing, oral presentation, CV + cover letter, job interview, and applying for a travel award. The participants responded very positively to the course.
Year(s) Of Engagement Activity 2019
URL https://acaciaafrica.org/afriplantsci/
 
Description Young Anglia Grows: LEP agri-tech 6th form taster course 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Myself, members of my lab, and a few colleagues entertained a group of ~10 year 12 pupils (top end of ability range) sourced by recommendations from Norfolk heads of biology. The day included a session on wheat diseases (formal lecture), a practical on using microscopes to observe wheat infected by stripe rust, a tour of the John Innes Centre and TGAC facilities, and a hands-on bioinformatics practical.
Year(s) Of Engagement Activity 2015
URL http://www.newanglia.co.uk/
 
Description Youtube video: "What is AgRenSeq" 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Ruby O'Grady in the JIC External Communications Team generated an animation to explain the meaning of the acronym "AgRenSeq".
Year(s) Of Engagement Activity 2019
URL https://www.youtube.com/watch?v=xefrjg5Y_Ug&t=6s