Response

Lead Research Organisation: John Innes Centre
Department Name: Contracts Office

Abstract

Genetic dissection of plant-microbe/pest interactions has led to the discovery of many key players in plant signal transduction. The challenge now is to link these components together, providing the detailed understanding of signal transduction mechanisms. A newly emergent area that we will focus on in the next 5 years is the cell-to-cell communication that allows the coordination of plant responses to pathogens, pests and symbionts. The technologies and understandings that we and others have developed over the last 5 years allow us to discriminate between the signal transduction mechanisms happening in different regions of the cell, in different cell types and at local versus systemic sites. Mathematical modelling will be incorporated with experimentation to explore these mechanisms of local and systemic signal propagation. There are several parallels between PAMP and symbiotic signaling and the work in this theme will allow us to compare and contrast these processes and to explore the mechanisms for differential activation of downstream responses.

PAMP-triggered immunity is associated with the production of a ROS burst, a calcium flux across the plasma membrane and the activation of a cytoplasmic kinase cascade. In contrast, symbiosis signaling involves oscillations of calcium in and around the nucleus that act as the primary mechanism of signal transduction. It is clear that ROS and calcium play important roles in intracellular signaling, but they also function in intercellular signaling. Differentiating between these local versus systemic responses is possible with better tools for measuring calcium and ROS changes.

Publications

10 25 50
 
Title Video showing how aphids sense aphid attack 
Description We launched a short video showing the method by which we can monitor the calcium elevations that occur in a leaf during aphid probing. 
Type Of Art Film/Video/Animation 
Year Produced 2018 
Impact The video will be understood by a wide audience. 
URL https://www.youtube.com/watch?v=CK2iIPfcnvo
 
Description Objective 2.1: We identified key components of the calcium signal machinery in response to aphid feeding. We demonstrated the interplay of the key components: the co-receptor BAK1, the plasma membrane ion channels GLR3.3 and GLR3.6, and the vacuolar calcium channel TPC1, thus linking signal recognition with response and signal transduction. In identifying core elements common to responses to a range of organisms, this study opens opportunities for developing an integrated understanding of plant responses to biotic stress. In further exploration of aphid responses, we optimized low-input RNA-seq methods (LORIS) to characterize the temporal and spatial dynamics of transcriptome changes that occur in and around aphid stylet penetration sites of Arabidopsis leaves. This is a collaboration with Matt Clark (Natural History Museum, London) and Iain Macaulay (Earlham Institute).
We have developed a computational framework for simulating, analysing and quantifying how different calcium signals (during plant-microbe interactions) can differentially active different sets of calcium decoders. During symbiotic relationships with plants, fungi channel inorganic and micronutrients from soil to the plant, and the plant supplies the fungi with organic nutrients. We demonstrated that as part of this exchange, the plant supplies lipids to its symbiotic fungi, thus providing the fungi with a robust source of carbon for their metabolic needs. This work shows that in addition to sugars, lipids are a major source of organic carbon delivered to the fungus, and this is necessary for production of fungal lipids. Calcium signalling is key to establishing symbiotic interactions and we generated a novel calcium reporter to visualise the calcium signal repartition between the cytoplasm and the nucleoplasm during this process. Using this new calcium reporter, we observed that symbiosis-induced nuclear calcium is released first into the nucleoplasm before diffusing to the cytoplasm identifying spatiotemporal details of this response.
Plant responses to microbes involve an array of gene expression changes. We have studied the role of chromatin remodelling in defense gene induction and found that the SWR1 chromatin remodelling complex and H2A.Z are essential for mounting full transcriptional response and for conferring resistance.

Objective 2.2: We identified that a calcium-responsive calmodulin-like protein (CML41) mediates flagellin-induced plasmodesmata closure. This protein is located at plasmodesmata, is calcium-responsive and positively regulates callose deposition. It links the regulation of cell-to-cell communication in response to bacteria to calcium signalling at plasmodesmata and presents a new tool from which to explore the role of cell-to-cell connectivity in immune responses. We made progress with characterizing the genetic components involved in the cell-to-cell spread of calcium waves that occur upon aphid feeding, and the study of whether these genetic components play similar roles in compatible and incompatible aphid-plant interactions. We developed a mathematical model for cell-cell communication during infection based on diffusion of a chemical agent through plasmodesmata.

Objective 2.3 Genetic loci affecting resistance to pathogens and immunity responses induced by PAMPs have been identified in Brassica napus using association genetics. The results form the basis of discussions about collaborative research on brassica pathology with industry. We also performed genome-wide association studies (GWAS) with NLP-induced ROS on a Brassica napus diversity panel, and identified novel QTL associated with quantitative and qualitative responses. We are currently in the process of identifying the underlying genes and are using Arabidopsis to determine whether they contribute to disease resistance.
Three Mlo homologues induced by mycorrhiza in barley have been identified and functional tests to investigate their role in mycorrhization are in progress. The results will improve understanding of the trade-off between disease resistance and mycorrhization in sustainable agriculture.
Exploitation Route This Theme of BBSRC's Institute Strategic Programme on Plant Health aims to determine the components and mechanisms by which plants transmit intra- and intercellular information in biotic interactions (pest, pathogen, symbiont). Understanding how plants carry out information signalling and processing is key for dissecting the molecular dialogue between plants and microbes and (in combination with outputs from the other themes) a first step towards the rational design of crop improvement for durable resistance and enhanced nutrient acquisition.
Sectors Agriculture, Food and Drink

 
Description Interactions with industry included presentations at a science innovation showcase (JIC), a workshop on conservation and organic agriculture, formal meetings, a crop walk with RMI analytics and the grain supply industry, a keynote presentation for RMI analytics symposium and a podcast with Dewing Grain Ltd . The findings were used to develop collaborations for exchange with industry, specifically with CN Seeds Ltd and Sarba Shrestha Seeds Pvt in Nepal funded by partners and International Flexible Interchange Project (BBSRC BB/S018972/1). A consultancy was established with Arlabion Ltd on priming of immune responses. ICASE PhD projects were established with CN Seeds Ltd and New Heritage Barley Ltd.
First Year Of Impact 2018
Sector Agriculture, Food and Drink
 
Description Project Leader of BRIGIT, a UK-wide consortium to mitigate the risks of Xylella fastidiosa outbreaks in the UK
Geographic Reach Europe 
Policy Influence Type Membership of a guideline committee
Impact The BRIGIT consortium includes people from various layers of government, charities, research institutes and industry. The writing of the BRIGIT proposal and activities within BRIGIT so far increased the knowledge of the consortium members about the Xylella pathosystem and how Xylella fastidiosa may spread in the UK and harm the environment. This is likely to influence future regulations to maximize protection of the UK environment.
URL https://www.jic.ac.uk/brigit/
 
Description PhD Student Fellowship
Amount £23,977 (GBP)
Organisation Sainsbury Research Unit 
Sector Academic/University
Country Unknown
Start 10/2018 
End 09/2022
 
Title Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells 
Description Spatiotemporal changes in cellular calcium (Ca2+) concentrations are essential for signal transduction in a wide range of plant cellular processes. In legumes, nuclear and perinuclear-localized Ca2+ oscillations have emerged as key signatures preceding downstream symbiotic signaling responses. Förster resonance energy transfer (FRET) yellow-based Ca2+ cameleon probes have been successfully exploited to measure the spatiotemporal dynamics of symbiotic Ca2+ signaling in legumes. Although providing cellular resolution, these sensors were restricted to measuring Ca2+ changes in single subcellular compartments. In this study, we have explored the potential of single fluorescent protein-based Ca2+ sensors, the GECOs, for multicolor and simultaneous imaging of the spatiotemporal dynamics of cytoplasmic and nuclear Ca2+ signaling in root cells. Single and dual fluorescence nuclear and cytoplasmic-localized GECOs expressed in transgenic Medicago truncatula roots and Arabidopsis thaliana were used to successfully monitor Ca2+ responses to microbial biotic and abiotic elicitors. In M. truncatula, we demonstrate that GECOs detect symbiosis-related Ca2+ spiking variations with higher sensitivity than the yellow FRET-based sensors previously used. Additionally, in both M. truncatula and A. thaliana, the dual sensor is now able to resolve in a single root cell the coordinated spatiotemporal dynamics of nuclear and cytoplasmic Ca2+ signaling in vivo. The GECO-based sensors presented here therefore represent powerful tools to monitor Ca2+ signaling dynamics in vivo in response to different stimuli in multi-subcellular compartments of plant cells. 
Type Of Material Physiological assessment or outcome measure 
Year Produced 2018 
Provided To Others? Yes  
Impact the cytoplasmic and nucleoplasmic dual sensors developed in this work are powerful tools to analyze the subcellular Ca2+ dynamics between the cytoplasm and nucleus in planta. These sensors will unequivocally be of interest in exploring Ca2+-mediated signaling in response to diverse stimuli in different plant species. 
URL https://www.frontiersin.org/articles/10.3389/fpls.2018.00245/full
 
Description Defence induction by Vial 8 
Organisation Arlabion Ltd
PI Contribution We performed analysis of a novel compound developed by the company to investigate its role in defence induction for disease control. We produced a report that they used for a patent application
Collaborator Contribution The partners provided the compound and advice about the nature of the chemical and the kind of analyses they required
Impact A report that was used for the patent application
Start Year 2018
 
Description Formal research collaboration with SESVanderHave 
Organisation Sesvanderhave
Country Belgium 
Sector Private 
PI Contribution My research group has provided advise on strategies to obtain aphid resistant sugar beet, exchanged knowledge on research progress in plant-insect interactions of the lab, and wrote BBRSC-LINK award to fund research.
Collaborator Contribution SESVanderHave provides access to sugar beet breeding lines, genome sequence resources for these lines and insecticide-free field sites for collection of aphid populations in UK and Europe. They also funded a postdoctoral researcher in my group for one year, contributed 50% in-kind funds for the BBSRC-LINK award and funds a iCASE studentship in my group. PhD student Roland Wouters was recruited for the iCASE project. Roland is making good progress.
Impact Generated knowledge on plant-insect interactions. Organized visits of the SESVanderHave team to JIC (two times per year) and my group at JIC to SESVanderHave headquarters in Tienen, Belgium (two times per year). Organized regular Skype calls to discuss research progress and ideas for future research.
Start Year 2013
 
Description Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis 
Organisation Ohio State University
Country United States 
Sector Academic/University 
PI Contribution Supervision of the study Symbioses experiments
Collaborator Contribution Study of the LINC components
Impact Newman-Griffis AH, Del Cerro P, Charpentier M, Meier I. Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis. Plant Physiology. 2019. 179(2):491-506
Start Year 2016
 
Description Plant Response Biotech 
Organisation Plant Response Biotech S.L.
Country Spain 
Sector Private 
PI Contribution This is an iCASE studentship with Plant Response Biotech. We have generated transgenic tomato homozygous lines expressing individual Arabidopsis receptor kinases belonging to the sub-family XII of LRR-RLKs. We have generated single and multiple insertional Arabidopsis mutants for LRR-RLK sub-family XII members.
Collaborator Contribution The lines generated above will be tested at the end of year at Plant Response Biotech by the student employed in this project for disease resistance. The partner will provide lab space, consumable costs, access to growth chambers, access to relevant pathogens, and access to proprietary plant-derived and microbial elicitors.
Impact not applicable yet.
Start Year 2015
 
Description Plant stimulants to prime plant immunity 
Organisation Arlabion Ltd
PI Contribution We provided our expertise to address a specific research question for the Company
Collaborator Contribution They provided the background and some technical knowledge to the project
Impact A report has been submitted to the company
Start Year 2017
 
Description Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells 
Organisation French National Institute of Agricultural Research
Department INRA Toulouse
Country France 
Sector Public 
PI Contribution We generated the Dual calcium sensor and the stably transformed plants (Arabidopsis thaliana and Medicago truncatula) We performed the research in Arabidopsis thaliana
Collaborator Contribution The Carvalho-Niebel lab generated DUAL sensor and stably transformed Medicago truncatula Performed the experiment in Medicago truncatula
Impact Kelner A, Leitao N, Chabaud M, Charpentier M*, De Carvalho-Niebel F*. Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells. Frontiers in Plant Science. 2018. 9:245.
Start Year 2017
 
Description The role of CML41 in flagellin induced plasmodesmata closure 
Organisation University of Adelaide
Department School of Agriculture, Food and Wine
Country Australia 
Sector Academic/University 
PI Contribution We provide expertise and technologies on the analysis of plasmodesmata in response to pathogens, live cell imaging and image analysis
Collaborator Contribution The Gilliham lab identified CML41 and performed gene expression analysis
Impact Publication: Xu B, Cheval C, Laohavisit A, Hocking B, Chiasson D, Olsson TSG, Shirasu K, Faulkner C, Gilliham M A calmodulin-like protein regulates plasmodesmal closure during bacterial immune responses. New Phytol. 2017 Jul;215(1):77-84. doi: 10.1111/nph.14599. Epub 2017 May 17.
Start Year 2015
 
Description A taste of Genetic Diversity: demonstration activity for the John Innes Centre open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact A demonstration of our research was performed at an open day at the John Innes Centre, involving hands on activities and discussion on the theme of 'a taste of genetic diversity. The activities involved showing how brassicas were used in baby leaf salads, and how heritage barley varieties were used in brewing beer
Year(s) Of Engagement Activity 2017
 
Description Breeders Day 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Breeders Day is an annual event to present current research on crop improvement at the John Innes Centre. The 2018 event was focussed on the supply chain, emphasising downstream processing of crops into products
Year(s) Of Engagement Activity 2018
 
Description Chair of Insectary Platform Steering Committee 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Chair of committee that ensures that the JIC Insectary/Entomology Technology Platform is well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs.
Year(s) Of Engagement Activity 2017,2018,2019
 
Description Chair of Technical Platform Oversight Committee (TPOC) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Chair the committee that ensures that all JIC Technology Platforms are collectively well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs.
Year(s) Of Engagement Activity 2017,2018,2019
 
Description Co-organiser of the satellite meeting on Signal Transduction Pathways in Nitrogen-Fixing Symbioses 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The meeting focused on molecular, genetic and in vivo dynamic aspects of plant symbiotic signaling, and offered the possibility to highlight the recent progress in Nitrogen-fixing signal transduction. This satellite meeting provided a privileged frame for presentations, discussions and exchanges around the latest scientific discoveries in plant signal transduction related to Nitrogen-fixing symbiosis, that were not covered during the 13rd European Nitrogen Fixation Conference (ENFC) in Stockholm, Sweden.
Year(s) Of Engagement Activity 2018
URL http://enfc2018.org/
 
Description Disease resistance and immunity in brassicas: presentation to the Chinese Academy of Agricultural Sciences 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation of our research on brassica pathology to the Chinese Academy of Agricultural Sciences. The meeting took place at the John Innes Centre
Year(s) Of Engagement Activity 2017
 
Description Disease resistance and immunity in brassicas: presentation to the Chinese Academy of Agricultural Sciences 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Study participants or study members
Results and Impact The annual meeting held with scientists from the Chinese Academy of Agricultural Sciences to present research updates and potential for collaborative research
Year(s) Of Engagement Activity 2018
 
Description Disease resistance research on brassicas 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Presentations were given to CN Seeds and Clover seeds about research on brassica disease resistance. The work has led to further development of collaborative research with CN Seeds on downy mildew in brassicas for the baby leaf salad market. We also made a successful joint application for GCRF-funded research to develop collaboration between CN Seeds and a seed company in Nepal.
Year(s) Of Engagement Activity 2018
 
Description Engagement with BBSRC as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Have weekly meetings with policy makers at BBSRC about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK
Year(s) Of Engagement Activity 2018,2019
 
Description Engagement with Defra, Forest Research and APHA as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Have weekly meetings with policy makers at Defra about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK
Year(s) Of Engagement Activity 2018,2019
 
Description Engagement with Fera as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Have weekly meetings with Fera about status of Xylella fastidiosa diagnostics that may influence new policies for reducing risk of X. fastidiosa outbreaks in the UK
Year(s) Of Engagement Activity 2018,2019
 
Description Engagement with Oxitec 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Regular meetings with Neil Morrison for supervisory meetings and discussing research progress of the iCASE studentship.
Year(s) Of Engagement Activity 2015,2016,2017,2018,2019
 
Description Engagement with Syngenta 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Regular meetings with colleagues at Syngenta, Jealott's Hill, UK, and Switserland and USA to discuss project proposals and research progress on aphids.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014
 
Description Engagement with the Royal Horticulticultural Society as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Third sector organisations
Results and Impact Have weekly meetings with staff member of RHS to develop a stakeholder engagement plan for BRIGIT
Year(s) Of Engagement Activity 2018,2019
 
Description Fast-Track to Quantitative Disease Resistance in Brassicas 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact This was a presentation it industry as part of the Science for Innovation Showcase at John Innes Centre. This was followed up by formal meetings with industry representative about potential future collaborative research
Year(s) Of Engagement Activity 2018
 
Description GCRF visit with Nepalese Researchers 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A fact-finding mission to Nepal was undertaken with the aim of developing new collaborations for potential GCRF projects
Year(s) Of Engagement Activity 2017
 
Description Genetic analysis of immune responses in brassicas 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation of research results at the 21st Crucifer Genetics Conference, St Malo, France. The audience was comprised of scientific researchers with a wide range of interests in crucifer genetics
Year(s) Of Engagement Activity 2018
URL https://colloque.inra.fr/brassica2018/
 
Description Hosted Alexander Stacey, BSc student Biological Sciences, University of East Anglia, UK. 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact Student worked on a research project in the summer of 2018 and his BSc research project from Jan-Mar '19.
Year(s) Of Engagement Activity 2018,2019
 
Description Immunity in brassicas 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Presentation to Elsoms, a seed and breeding company interested in collaborative research on oilseed rape improvement, especially in disease resistance
Year(s) Of Engagement Activity 2018
 
Description Improving disease control in brassicaas 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Meeting with Prime agriculture to present research and explore potential collaborations in the future. Prime Agriculture are consultants in agriculture, with particular focus on agronomy. The work has led to an application to assess research priorities for disease control research in brassicas
Year(s) Of Engagement Activity 2018
 
Description Invited Seminar at Bristol University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited seminar speaker at Bristol University - title 'The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?'
Year(s) Of Engagement Activity 2017
 
Description Invited Speaker: Host Microbe Interactions- Norwich Research Park Group 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Discussion within the Norwich research park scientists working on Host-Microbe Interactions in plant and animal.
Year(s) Of Engagement Activity 2018
 
Description Invited plenary speaker: 13th European Nitrogen Fixation Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Prolific discussions and interest in nitrogen fixation signaling, as well as in promoting equality and diversity in science
Year(s) Of Engagement Activity 2018
 
Description Invited seminar at the 3rd Adam Kondorosi Symposium: Frontiers in Beneficial Plant Microbe Interactions 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The objective of this Symposium was to create a scientific event that is at the forefront of fundamental research in beneficial plant-microbe interactions.
The Symposium brought together about 150 participants in a rather informal atmosphere, facilitating exchanges. This promoted exciting discussion with researchers - in particular those at the early stage of their career.
Year(s) Of Engagement Activity 2017
URL https://symposium.inra.fr/ak-ips2-2017_eng/
 
Description Invited speaker - 'When development meets stress' B-Debate, Barcelona, Spain 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I participated in, and presented our reserach, in this cross-field debate in which researchers from developmental and stress biology met to discuss trade-offs and other issues that effect both areas of biology.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker - International Microscopy Congress 19, Sydney, Australia 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I presented our work and live-imaging technologies at this technology focussed congress.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker - PDRA/PGR Symposium, University of Glasgow UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was an invited speaker at the University of Glasgow PDRA/postgraduate student research symposium. I presented my research and career path ot the audience.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker - VISCEA Plant Biotic Stresses and Resistance Mechanisms III, Vienna, Austria 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I presented my research to, and engaged in discussions with, participants at this symposium. This lead to some fruitful discussions regarding the similarities between my system and those of other researchers (i.e. plasmodesmata v extracellular vesicles)
Year(s) Of Engagement Activity 2018
 
Description Invited speaker at York University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited speaker to York University: Plant Biology Seminar - The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2017
 
Description Invited speaker at the XIX International Botanical Congress, Shenzhen, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Invited speaker at on Calcium transport and signaling.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker: Gordon Research Conference on Organellar Channels and Transporters 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited speaker: Gordon Research Conference on Organellar Channels and Transporters Gordon Research Conference on Organellar Channels and Transporters
Year(s) Of Engagement Activity 2017
 
Description Invited speaker: Gordon Research Conference on Organellar Channels and Transporters, Mount Snow, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This dedicated conference provided a unique opportunity for animal and plant scientists working on different organelles to share data and ideas to help addressing current challenges of organelle biology, foster collaborations, and identify new ways to treat common and rare diseases based on better understanding of mechanisms of ion/metabolite transport in various organelles.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker: International Plant Molecular Biology Conference, Montpellier, France 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact stimulated discussion promoting new collaboration.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker: Max Planck Institute for Plant Breeding Research, Cologne, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Exciting discussion promoting new collaboration
Year(s) Of Engagement Activity 2019
 
Description Invited speaker: Max Planck Institute of Molecular Plant Physiology, Golm, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Discussion with scientists to increase collaborative effort aiming at improving crop production
Year(s) Of Engagement Activity 2018
 
Description Invited speaker: SEB Functional organisation of the nuclear periphery, Florence, Italy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This session included presentations on recent advances in knowledge and understanding molecular functions, dynamics and interactions of proteins of the nuclear envelope across kingdoms; topics will include the proteins of the linker of cytoskeleton and nucleoskeleton complex; the nuclear pore complex; the nucleoskeleton and lamina and the interactions of the nuclear envelope with the cytoskeleton. It included applications ranging from the role of nuclear envelope proteins in plant stress and disease responses to developmental disorders caused by laminopathies. The session generated very stimulated discussions.
Year(s) Of Engagement Activity 2018
 
Description Invited talk - University of Sheffield, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I presented our research and spent time with researchers and students in one to one meetings.
Year(s) Of Engagement Activity 2018
 
Description JIC50 public open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Open Day at JIC celebrating 50 years on the Norwich site. Soil columns featuring the N sensors were displayed to the public.
Year(s) Of Engagement Activity 2017
URL https://www.jic.ac.uk/news-and-events/blog-copy/2017/08/john-innes-celebrates-50-years-norwich-2017
 
Description Keynote Speaker - EPS Get2gether, PhD student symposium, Netherlands 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I presented my research and career progression to postgraduate students in the EPG Graduate School. After my presentation I engaged in discussion with students and a number were very interested in my career path.
Year(s) Of Engagement Activity 2018
 
Description Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit
Year(s) Of Engagement Activity 2017
 
Description Main organiser - EMBO workshop on Intercellular Communication in Development and Disease, Berlin, Germany 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact As main organiser of this workshop I secured funding from EMBO and shaped the programme of this workshop. We invited speakers from broad geographical locations and topic areas. The workshop feedback was overwhelmingly positive with a number of participants describing how they had established new collaborations from attending this workshop. The workshop has been passed on to a new group of organisers to arrange for a repeat event in 2020.
Year(s) Of Engagement Activity 2017
 
Description Mechanistic Analysis of Quantitative Disease Resistance in Brassica by Associative Transcriptomics 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Study participants or study members
Results and Impact This was the final workshop of MAQBAT to discuss results and plans for publications
Year(s) Of Engagement Activity 2018
 
Description Member Scientific Resources Committee 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Member of a committee that decides on purchases of all types of scientific equipment and organization of JIC infrastructure.
Year(s) Of Engagement Activity 2017,2018,2019
 
Description Member of the Synthetic Biology Leadership Council 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Member of the Synthetic Biology Leadership Council
Year(s) Of Engagement Activity 2017
 
Description Member of the UK Science Partnership for Animal and Plant Health 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Member of the UK Science Partnership for Animal and Plant Health - quarterly meetings are held
Year(s) Of Engagement Activity 2017
 
Description Member of the interviewing panel for Herchel Smith Professorship of Biochemistry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Member of the interviewing panel for Herchel Smith Professorship of Biochemistry position - University of Cambridge
Year(s) Of Engagement Activity 2017
 
Description Membership of Scientific Advisory Committee to the Plant Energy Biology ARC Centre of Excellence 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Membership of Scientific Advisory Committee to the Plant Energy Biology ARC Centre of Excellence. An Australian National Centre.
Year(s) Of Engagement Activity 2017
 
Description New Phytologist Next Generation Scientists meeting - Open Address 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Opening Address at New Phytologist Next Generation Scientists meeting - Scientific meeting
Year(s) Of Engagement Activity 2017
 
Description Opening address at Breeders Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Opening address at Breeders Day at the John Innes Centre
Year(s) Of Engagement Activity 2017
 
Description Organization of the International Plant calcium signalling conference 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This conference brought all the plant calcium community together. It triggered very stimulating and interesting discussions and new opportunities of collaboration for many attendees.
Year(s) Of Engagement Activity 2017
URL http://plantcalciumsignalling.com/
 
Description PAMP responses in the Triangle of U 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact presentation of results from the MAQBAT project to update the brassica community about progress with the work. The presentation has enable further discussions about potential future collaborations and project ideas
Year(s) Of Engagement Activity 2018
 
Description Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain
Year(s) Of Engagement Activity 2017
 
Description Panel discussion for the Royal Society for Hay Festival 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Hay Festival - panel discussion for the Royal Society
exploring the public attitudes to the range of applications that genetic technologies might have, and the differences in such attitudes depending on context. This might look at the difference in public opinion in areas like food and medicine, for example, or explore concepts like 'naturalness'
Year(s) Of Engagement Activity 2017
 
Description Plant Calcium Signalling Meeting CHANNELS THAT REGULATE CALCIUM MOVEMENT 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Free Calcium: A Paradigm for Ca2+ induced Ca2+ Release in Plants? Presentation at Plant Calcium Signalling Meeting CHANNELS THAT REGULATE CALCIUM MOVEMENT
Year(s) Of Engagement Activity 2017
 
Description Presented talk at JIC "Science for Innovation Showcase" event 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presented a talk at the JIC "Science for Innovation Showcase" event, Norwich, UK, 7-8 Feb '18. I explored opportunities to collaborate with industry.
Year(s) Of Engagement Activity 2018
 
Description Press release to announce BRIGIT project 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Engaged with JIC Public Engagement Officer to launch press release 'UK consortium to combat serious threat to plant health' to announce the start of the BRIGIT project and the BRIGIT project website
Year(s) Of Engagement Activity 2018
URL https://www.jic.ac.uk/press-release/uk-wide-consortium-to-combat-serious-threat-to-plant-health/
 
Description Press release to announce new appointment for the BRIGIT project 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Engaged with JIC Public Engagement Officer to launch press release 'New appointment for UK-wide Xylella pathogen consortium' for the BRIGIT project
Year(s) Of Engagement Activity 2019
URL https://www.jic.ac.uk/news/new-appointment-for-uk-wide-xylella-pathogen-consortium/
 
Description PrimeAg visit 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Eight visitors from PrimeAg visited JIC for half a day. Prime Agriculture LLP is a consultancy partnership of 11 BASIS and FACTS qualified agronomists, working with growers in in Essex, Suffolk, Norfolk, Cambridgeshire, Bedfordshire, Northamptonshire and Hertfordshire. http://www.primeag.co.uk/
Year(s) Of Engagement Activity 2018
URL http://www.primeag.co.uk/
 
Description Royal Society fact finding visit to Beijing 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Royal Society fact finding visit to Beijing, 22 to 26 October for Royal Society's genetic technologies programme
Year(s) Of Engagement Activity 2017
 
Description Science Breaker - How plants breathe 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Working with engagement specialists, we developed an online 'Science Breaker' which explains the science to the general public and acts simultaneously as a forum for questions and answers.
Year(s) Of Engagement Activity 2017
 
Description Scientific Advisory Board Member for Max PIanck Institute of Molecular Plant Physiology, Golm, Germany 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Scientific Advisory Board Meeting for Max PIanck Institute of Molecular Plant Physiology
Year(s) Of Engagement Activity 2017
 
Description Transatlantic Sessions in Conservation and Organic Agriculture 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact We held a workshop to discuss current thinking in conservation and organic agriculture, with speakers from UK and USA. This was an opportunity to discuss potential collaborations, and future joint research. These discussions are ongoing, though no specific projects have yet materialised
Year(s) Of Engagement Activity 2018
 
Description University of Geneva - PhD Retreat 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact University of Geneva - PhD Retreat, participation in a debate: " Technological advances - A new chance for changing public perception on GMOs". Also speaker title: 'The plant lytic vacuole: space-filler, garbage bag, or something more interesting'. A two-day retreat for PhD students in molecular plant sciences at universities in French-speaking Switzerland.
Year(s) Of Engagement Activity 2017
 
Description presentsation to the Flying Farmers 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Flying farmers are a group of farmers interested to learn about the latest trends in research. the presentation involved talking to them about our work, and engaging in a discussion with them
Year(s) Of Engagement Activity 2017