Protein Manipulation in Lipid Bilayers using Surface Acoustic Waves
Lead Research Organisation:
University of Leeds
Department Name: Physics and Astronomy
Abstract
The phospholipid bilayer that separates the internal cellular environment from the outside world presents an impervious barrier for the transport of large molecules and charged ions. Proteins located within this bilayer provide a mechanism for the passage of nutrients and waste products between the cytoplasm and the external environment. Further, they also play pivotal role in transmitting the cellular response to chemical changes such as the interaction with hormones, toxins, etc. It is not surprising that such proteins are of significant pharmaceutical interest. Significant progress in understanding the function of many membrane proteins has been hampered since they often change their structure, and lose their functionality, once removed from their membranous environment. Our group has a strong interest in developing new platforms and tools for studying such bilayer membranes and proteins. Here we propose to develop a new technique for manipulating such proteins whilst keeping them in their lipid bilayer environment. In particular, we wish to use the electric fields associated with evanescent waves (created using a modified surface acoustic wave device) to move charged proteins within the lipid bilayer. This represents the first step towards the facile manipulation of membrane proteins and which will eventually allow us to trap, move and separate proteins within the bilayer. The will be a significant breakthrough for the study of membrane proteins.
Publications
Achalkumar A
(2011)
Synthesis of nitrilotriacetic acid terminated tethers for the binding of His-tagged proteins to lipid bilayers and to gold
in Tetrahedron
Achalkumar A
(2010)
Cholesterol-based anchors and tethers for phospholipid bilayers and for model biological membranes
in Soft Matter
Al-Lawati Z
(2013)
Alignment of a Columnar Hexagonal Discotic Liquid Crystal on Self-Assembled Monolayers
in The Journal of Physical Chemistry C
Bao P
(2012)
On-chip alternating current electrophoresis in supported lipid bilayer membranes.
in Analytical chemistry
Benz F
(2015)
Generalized circuit model for coupled plasmonic systems.
in Optics express
Benz F
(2015)
Nanooptics of molecular-shunted plasmonic nanojunctions.
in Nano letters
Blakeston AC
(2015)
New poly(amino acid methacrylate) brush supports the formation of well-defined lipid membranes.
in Langmuir : the ACS journal of surfaces and colloids
Cheetham M
(2012)
Manipulation and sorting of membrane proteins using patterned diffusion-aided ratchets with AC fields in supported lipid bilayers
in Soft Matter
Cheetham MR
(2011)
Concentrating membrane proteins using asymmetric traps and AC electric fields.
in Journal of the American Chemical Society
Cheneler D
(2011)
Characteristics and durability of fluoropolymer thin films
in Polymer Degradation and Stability
Cheng N
(2015)
Facile Formation of Highly Mobile Supported Lipid Bilayers on Surface-Quaternized pH-Responsive Polymer Brushes
in Macromolecules
Daskalakis N
(2011)
Driving bioenergetic processes with electrodes
in Soft Matter
Daskalakis N
(2011)
Vesicle-modified electrodes to study proton-pumping by membrane proteins
in Electrochimica Acta
Dobrzanska DA
(2013)
Oxidation of tertiary amine-derivatized surfaces to control protein adhesion.
in Langmuir : the ACS journal of surfaces and colloids
Han X
(2010)
A self-assembly route for double bilayer lipid membrane formation.
in Chemphyschem : a European journal of chemical physics and physical chemistry
Kendall JK
(2010)
Effect of the structure of cholesterol-based tethered bilayer lipid membranes on ionophore activity.
in Chemphyschem : a European journal of chemical physics and physical chemistry
Kong Y
(2016)
Highly Fluorescent Ribonuclease-A-Encapsulated Lead Sulfide Quantum Dots for Ultrasensitive Fluorescence in Vivo Imaging in the Second Near-Infrared Window.
in Chemistry of materials : a publication of the American Chemical Society
Krzeminski L
(2011)
Spectroelectrochemical investigation of intramolecular and interfacial electron-transfer rates reveals differences between nitrite reductase at rest and during turnover.
in Journal of the American Chemical Society
Krzeminski L
(2011)
Orientational control over nitrite reductase on modified gold electrode and its effects on the interfacial electron transfer.
in The journal of physical chemistry. B
McMillan DG
(2013)
Protein-protein interaction regulates the direction of catalysis and electron transfer in a redox enzyme complex.
in Journal of the American Chemical Society
O'Rorke R
(2012)
Acousto-microfluidics: Transporting microbubble and microparticle arrays in acoustic traps using surface acoustic waves
in Journal of Applied Physics
Prompinit P
(2010)
Controlling liquid crystal alignment using photocleavable cyanobiphenyl self-assembled monolayers.
in ACS applied materials & interfaces
| Description | Through this research we integrated on chip surface acoustic waves into microfluidic systems for the manipulation of particles and bubbles in the size regime 1 to 20 um. We were able to demonstrate that we could use SAW to forma standing wave to : i) create particles arrays - test-tubes without walls ii) move particles in arrays in sequential manner iii) use SAW device as a mixer |
| Exploitation Route | For lab on chip device / manipulation of micro bubbles for separation / cleaning after functionalisation |
| Sectors | Aerospace Defence and Marine Agriculture Food and Drink Chemicals Education Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
| Description | The findings of this work have so far been primarily of academic impact. It has contributed to the growing amount of research on the integration of SAW devices into microfluidics. Translation of the impact of this into the CSEP options below is likely to be over many years. |
| First Year Of Impact | 2005 |
| Sector | Healthcare |
| Impact Types | Cultural |