Patterning Biological Cells Using Microfabrication Technology

Lead Research Organisation: University of Edinburgh
Department Name: Sch of Engineering

Abstract

The project will take a technology that has been developed under previous EPSRC fuinding and prepare it for patenting and commercialisation.The technique involves simple microfabrication (chip manufacture) technology that is commonplace and inexpensive in the semiconductor cleanroom. Patterns are created on silicon, using standard microfabrication and carefully-chosen materials (polymers). These patterns are, at the time of creation, biologically inert. They can be stored as blanks indefinitely.Subsequently, the blanks are dipped in a protein mixture that activates them - whereupon neural cells grow preferentially on the patterns of polymer, allowing networks to be designed and createwd with considerable accuracy.This project will optimise the microfabrication steps and bioligical dipping process for neurones, explore the ability to pattern other forms of cell such as stem cells and muscles and build a business case for exploiting the technology.Application areas include:-1) Laboratory expermintation using designed patterns of cells2) Drug discovery and assay3) Prosthetics - using patterns to effectively re-wire damaged tissue(1) is an immediate prospect. (2) will be the focus of a market evaluation and business plan during this follow-on project, while (3) is a considerably longer-term prospect.

Publications

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Delivopoulos E (2010) Effects of parylene-C photooxidation on serum-assisted glial and neuronal patterning. in Journal of biomedical materials research. Part A

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Hughes MA (2014) Cell patterning on photolithographically defined parylene-C: SiO2 substrates. in Journal of visualized experiments : JoVE

 
Description See "Narrative impact" above. This was a follow-on project and therefore did not aim to make new scientific discoveries.
Exploitation Route Via a project to explain the surface chemistry that gives rise to parylene patterning and thus the route to a reliable, high-yield process that could be tuned to different cell types.
Sectors Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description The primary finding was that potential industrial users felt they needed the patterning technique to be fully understood scientifically (ie why do cells pattern on parylene?) in order to develop a controllable and high-yield process. As a result, no users took the work forward and the team submitted a proposal to EPSRC to build this understanding. The proposal was not funded and this work is currently stalled.
First Year Of Impact 2009
Impact Types Economic

 
Description University of Auckland 
Organisation University of Auckland
Country New Zealand 
Sector Academic/University 
PI Contribution Dr. Charles Unsworth was funded to travel and work in Edinburgh and this collaboration has generated a long-term sustainable partnership between the Universities of Edinburgh and Aukland.
Collaborator Contribution Dr Unsworth manufactured silicon substrates for patterning in Edinburgh and subsequently collaborated with medical researchers in Auckland to show that human cells can be patterned on these substrates.
Impact Many research papers and an EPSRC proposal (unfunded) to better understand the mechanism behind cell patterning by parylene on silicon.
Start Year 2009
 
Title Cell patterning 
Description  
IP Reference GB0820572.6 
Protection Patent application published
Year Protection Granted
Licensed No