Luminescent nanoparticles as trackers for imaging of flows and sensing phenomena in microchannels
Lead Research Organisation:
University College London
Department Name: Chemical Engineering
Abstract
Fluorescence imaging has become an increasingly appealing technique for detection because it is highly sensitive as well as non-invasive and non-destructive, providing good temporal resolution for detection of fast events. One of the challenges in optical imaging is to increase spatial resolution; this would require decrease in probe size combined with the ability to detect the individual fluorescent probes. In this proposal, through an interdisciplinary approach, we aim to develop novel Luminescent Nanosized tracking probes (LNt), study the images of these single particles in static and flow conditions and use the LNt for resolving velocity and concentration profiles in micron sized channels (the latter using sensing at the nanoparticle level). The LNt will be prepared by the attachment of lanthanide and ruthenium luminescent complexes on gold and platinum nanoparticles. These particles will luminesce in the visible and near infra-red providing different colours for detection and their images will be obtained together with spectral information of each nanoparticle, which will allow colour recognition. We will apply the LNt to investigate flow and reactive systems with micron sized features. These studies will provide a breakthrough in the analysis of miniaturized chemical and biological systems because they will enable simultaneous velocity and concentration measurements with very high spatial resolution that will allow submicron scale phenomena to be resolved The luminescent properties of LNt are defined by the photophysical properties of the molecular label the particles are coated with. The lanthanide light emission is far from the light excitation wavelength, which avoids any interference of scattering light. Emission in infra red is also possible which is transparent to skin and for blood flow this is an advantage as several blood pigments absorb the visible radiation emitted from common lumophores. The nanoparticles developed will be used to investigate the complex sub-micron scale flows that can appear due to Marangoni phenomena during CO2 absorption in amine solutions in micro-chemical units. The feasibility of using the LNt to monitor blood flows will also be evaluated. LNt can be tailored-made for sensing different molecules, which gives them a built-in ability to sense specific chemical species and be uniquely used for both concentration and velocity measurements. Apart from lanthanides we will also be using ruthenium bipyridyl luminescent complexes which are attractive because their luminescence is sensitive to the presence of oxygen and oxygen concentration. Velocity and concentration profile measurements will be demonstrated for microprocesses using lanthanide and ruthenium LNt that can sense small molecules (aromatic acids) and oxygen respectively. The proof of principle of the application of LNt for flow velocity and concentration measurements had been demonstrated in a recently completed Discipline Hopping project between Chemistry and Chemical Engineering awarded to the two Principal Investigators.
Publications

Lewis DJ
(2013)
Silica nanoparticles for micro-particle imaging velocimetry: fluorosurfactant improves nanoparticle stability and brightness of immobilized iridium(III) complexes.
in Langmuir : the ACS journal of surfaces and colloids

Li Q
(2016)
Intensified Eu(III) extraction using ionic liquids in small channels
in Chemical Engineering Science

Tsaoulidis D
(2014)
{UO2}2+ Extraction Using Ionic Liquids in Intensified Extractors


Tsaoulidis D
(2015)
Effect of channel size on mass transfer during liquid-liquid plug flow in small scale extractors
in Chemical Engineering Journal

Tsaoulidis D
(2016)
Effect of channel size on liquid-liquid plug flow in small channels
in AIChE Journal

Tsaoulidis D
(2013)
Flow patterns and pressure drop of ionic liquid-water two-phase flows in microchannels
in International Journal of Multiphase Flow
Description | Luminescent nano particle trackers were developed together with laser based optical techniques to monitor flow phenomena in small channels. The results made possible investigations of complex two-phase flows in small channels that have since been used for many processes. Examples include intensified separations of metals using ionic liquids as alternatives to green solvents and flows with non-Newtonian fluids which find applications ion recovery and production of food and consumer goods. |
Exploitation Route | Both the luminescent nanoparticles and the optical experimental methodologies developed can be used to investigate two-phase systems. |
Sectors | Chemicals,Energy,Environment,Healthcare,Pharmaceuticals and Medical Biotechnology |
URL | https://www.ucl.ac.uk/chemical-engineering/thames-advanced-multiphase-systems |
Description | Demonstrations to school children of micro channel flows. Talks to school children demonstrating the results of the research and the industrial applications. The emphasis was on the importance of the research for the development of intensified and sustainable processes. |
First Year Of Impact | 2011 |
Sector | Chemicals,Energy,Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Societal,Economic |
Description | Complex ORAL health products (CORAL): Characterisation, modelling and manufacturing challenges |
Amount | £1,945,935 (GBP) |
Funding ID | EP/N024915/1 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2016 |
End | 08/2020 |
Description | EPSRC Programme grant MEMPHIS |
Amount | £1,082,478 (GBP) |
Funding ID | EP/K003976/1 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2012 |
End | 09/2017 |
Description | Fellowship |
Amount | £48,531 (GBP) |
Organisation | Royal Academy of Engineering |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 09/2011 |
End | 09/2012 |
Description | Integrated Experimental And Computational Fluid Dynamic Studies For Advanced Process Development Of Complex Oral Health Products |
Amount | £21,498 (GBP) |
Organisation | GlaxoSmithKline (GSK) |
Sector | Private |
Country | Global |
Start | 08/2014 |
End | 09/2017 |
Description | Mixing in complex oral health products |
Amount | £33,000 (GBP) |
Organisation | GlaxoSmithKline (GSK) |
Sector | Private |
Country | Global |
Start | 08/2016 |
End | 08/2019 |
Description | PREdictive Modelling with QuantIfication of UncERtainty for MultiphasE Systems (PREMIERE) |
Amount | £6,560,538 (GBP) |
Funding ID | EP/T000414/1 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2019 |
End | 09/2024 |
Description | IProPBio |
Organisation | Federal University of Paraná |
Country | Brazil |
Sector | Academic/University |
PI Contribution | Expertise on intensified processes and particularly on liquid-liquid reactions and separations. Access to equipment for intensified processes. |
Collaborator Contribution | Knowledge of thermodynamics, phase equilibrium measurements and thermodynamic modeling. Also process synthesis and optimization for the design of biorefineries |
Impact | This is an international collaboration with partners from Europe, US and Brazil. The consortium has formed to apply for funding for the design, optimization and operation of sustainable biorefineries for multi product portfolios. |
Start Year | 2017 |
Description | IProPBio |
Organisation | University of Patras |
Department | Department of Mechanical Engineering and Aeronautics |
Country | Greece |
Sector | Academic/University |
PI Contribution | Expertise on intensified processes and particularly on liquid-liquid reactions and separations. Access to equipment for intensified processes. |
Collaborator Contribution | Knowledge of thermodynamics, phase equilibrium measurements and thermodynamic modeling. Also process synthesis and optimization for the design of biorefineries |
Impact | This is an international collaboration with partners from Europe, US and Brazil. The consortium has formed to apply for funding for the design, optimization and operation of sustainable biorefineries for multi product portfolios. |
Start Year | 2017 |
Description | IProPBio |
Organisation | University of Southern Denmark |
Country | Denmark |
Sector | Academic/University |
PI Contribution | Expertise on intensified processes and particularly on liquid-liquid reactions and separations. Access to equipment for intensified processes. |
Collaborator Contribution | Knowledge of thermodynamics, phase equilibrium measurements and thermodynamic modeling. Also process synthesis and optimization for the design of biorefineries |
Impact | This is an international collaboration with partners from Europe, US and Brazil. The consortium has formed to apply for funding for the design, optimization and operation of sustainable biorefineries for multi product portfolios. |
Start Year | 2017 |