Nanoactuation of plasma membrane protein clusters

Lead Research Organisation: Nottingham Trent University
Department Name: School of Science & Technology

Abstract

Plasma membrane proteins show complex dynamic ordering, such as clustering, on a nanoscale level, which typically changes after external stimulation. The mechanisms of these processes and the consequences for signal transduction are of great interest as extra-cellular signaling events are translated into cells via the plasma membrane. We aim to actuate plasma membrane proteins in a controlled fashion using rationally designed nano-objects and to visualize this interfacing process on the nanometer scale, yielding new insights into cell signalling via the plasma membrane. Rationally designed nano-objects with controlled interfaces to actuate proteins in membranes specifically address the call requirement for groundbreaking research in nanoscale interfaces. The nano-objects bridge the gap between studies on isolated proteins that cannot account for protein clustering in the native environment and whole cell studies, that do not allow the controlled actuation of the nano-clusters.
 
Description The project developed technologies for assembling and enumerating clusters of proteins. We discovered that a formalism originally developed in the 1960s needed to be refined to take into account the real behaviour of fluorescent dyes.
Exploitation Route It is anticipated that this work will be used by researchers using fluorescence anisotropy methods to understand protein cluster formation and related assemblies. The theoretical framework developed and the new systems created for precisely assembling fluorophores will enable new capabilities in cell imaging.
Sectors Education,Pharmaceuticals and Medical Biotechnology,Other

 
Description The publications are relatively new, but they are beginning to be cited.
First Year Of Impact 2014
Sector Education
 
Description NanoActuate NanoScience E+ Consortium 
Organisation Eindhoven University of Technology
Country Netherlands 
Sector Academic/University 
PI Contribution This grant was funded via the NanoScience E+ scheme and includes: Nottingham Trent University The University of Twente, Netherlands The Technische Universiteit Eindhoven, The Netherlands The Max Planck Institute for Molecular Biology, Dortmund, Germany. The four named individuals at the respective institutions began working together in 2008. Collaboration has included exchange of students and International visits between institutions.
Start Year 2008
 
Description NanoActuate NanoScience E+ Consortium 
Organisation Max Planck Society
Department Max Planck Institute for Molecular Cell Biology and Genetics
Country Germany 
Sector Public 
PI Contribution This grant was funded via the NanoScience E+ scheme and includes: Nottingham Trent University The University of Twente, Netherlands The Technische Universiteit Eindhoven, The Netherlands The Max Planck Institute for Molecular Biology, Dortmund, Germany. The four named individuals at the respective institutions began working together in 2008. Collaboration has included exchange of students and International visits between institutions.
Start Year 2008
 
Description NanoActuate NanoScience E+ Consortium 
Organisation Nottingham Trent University
Country United Kingdom 
Sector Academic/University 
PI Contribution This grant was funded via the NanoScience E+ scheme and includes: Nottingham Trent University The University of Twente, Netherlands The Technische Universiteit Eindhoven, The Netherlands The Max Planck Institute for Molecular Biology, Dortmund, Germany. The four named individuals at the respective institutions began working together in 2008. Collaboration has included exchange of students and International visits between institutions.
Start Year 2008
 
Description NanoActuate NanoScience E+ Consortium 
Organisation University of Twente
Country Netherlands 
Sector Academic/University 
PI Contribution This grant was funded via the NanoScience E+ scheme and includes: Nottingham Trent University The University of Twente, Netherlands The Technische Universiteit Eindhoven, The Netherlands The Max Planck Institute for Molecular Biology, Dortmund, Germany. The four named individuals at the respective institutions began working together in 2008. Collaboration has included exchange of students and International visits between institutions.
Start Year 2008