EPSRC-Royal Society Fellowship Engagement (2013): Principles of Early T-Cell Activation using Quantitative 3D Super-Resolution Nanoscopy
Lead Research Organisation:
University of Cambridge
Department Name: Chemistry
Abstract
Please refer to attached Royal Society application
Planned Impact
Please refer to attached Royal Society application
Publications
Basu S
(2018)
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
in Nature Communications
Carr A
(2016)
3D Super-Resolution Imaging of Unperturbed Cells
in Biophysical Journal
Carr AR
(2017)
Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function.
in Biophysical journal
Etheridge TJ
(2014)
Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy.
in Nucleic acids research
Felce JH
(2017)
Receptor Quaternary Organization Explains G Protein-Coupled Receptor Family Structure.
in Cell reports
Fritzsche M
(2015)
CalQuo: automated, simultaneous single-cell and population-level quantification of global intracellular Ca2+ responses.
in Scientific reports
Klehs K
(2014)
Increasing the brightness of cyanine fluorophores for single-molecule and superresolution imaging.
in Chemphyschem : a European journal of chemical physics and physical chemistry
| Description | This project has led to publication of multiple nature immunology papers, most notably "Initiation of T cell signaling by CD45 segregation at 'close contacts' Nature immunology 17 (5), 574-582, 2016 - 162 citations. This for the first time revealed the existence of 'close' contacts in the molecular origin of t-cell triggering. This publication address arguably the most important question in immunology that is "How is the T cell triggered?" This paper presents strong data for the "kinetic segregation model" first posed in the early 90s, but until now the ,methods where not sufficient to visualise this process. We have achieved this this is important in Cancer work as well as autoimmune disease |
| Exploitation Route | We are continuing to use build on this work. however attempting to visualising close contacts in real cell-cell contacts is the next step. We are currently using advanced microscopy methods to address this |
| Sectors | Healthcare |
| URL | https://www.nature.com/articles/ni.3392 |
| Description | Science-Museum Lates |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | We are involved with the Science Museum Lates which a student funded by this grant will participate, as well as a successful grant from the royal society Named "How will a Gin a tonic save your life?" focusing on super-resolution fluorescence imaging and it's application to the human adaptive immune system. |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://www.sciencemuseum.org.uk/visitmuseum/plan_your_visit/lates |