Cardiff University Experimental Equipment

Lead Research Organisation: Cardiff University
Department Name: Research and Commercial Division

Abstract

N/A - see case for support

Planned Impact

N/A - see case for support

Publications

10 25 50
 
Description With this award we have designed and built a 'third generation' multiphoton microscope which enables label-free chemically-specific Coherent Raman Scattering (CRS) microscopy in living cells and tissues over a wider vibrational frequency range and with reduced photodamage compared to previous generation systems. CRS functionalities are complemented by confocal point and line-scan Raman imaging. The multimodal platform also enables label-free second harmonic generation (SHG) and third-harmonic generation (THG) imaging, as well as background-free sensitive imaging of single small metallic nanoparticles via resonant four-wave mixing (FWM) in the same instrument. Moreover, it includes state-of-the art fluorescence modalities, namely two-photon fluorescence (TPF), fluorescence lifetime imaging (FLIM), fluorescence correlation spectroscopy (FCS), as well as super-resolution microscopy.
Exploitation Route This platform will enable rapid label-free chemically-specific microscopy of biomaterials (lipids, proteins, DNA) and advanced materials including nanodiamonds, carbon nanotubes, graphene, polymers, and semiconductor nanostructures. It will also enable background-free microscopy of single metallic nanoparticles, even when embedded in highly scattering/fluorescing environments such as cells and tissues, and fluorescence microscopy of labelled biomolecules including super-resolution single-molecule localisation microscopy. These capabilities will support on-going research at the forefront of existing technologies across several major groups at Cardiff University, and in the UK.
Sectors Pharmaceuticals and Medical Biotechnology

 
Description The imaging and research capabilities enabled by this equipment have been used to strengthen collaborations with microscope manufacturer and pharmaceutical companies in the UK and Europe. A number of grant funding applications for collaborative projects with companies have been submitted.
First Year Of Impact 2017
Sector Pharmaceuticals and Medical Biotechnology
Impact Types Economic

 
Description A label-free tool to unravel the dynamics of lipid bilayers containing single membrane proteins: iGOR microscopy
Amount £151,136 (GBP)
Funding ID BB/R021899/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 02/2019 
End 04/2020
 
Description Lipid droplets in oocytes: shedding new light on why fats are good or bad for development.
Amount £472,355 (GBP)
Funding ID BB/P007511/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2017 
End 03/2020
 
Description Royal Society Challenge Grant Awards
Amount £91,269 (GBP)
Funding ID CH160031 
Organisation The Royal Society 
Sector Academic/University
Country United Kingdom
Start 12/2016 
End 12/2017
 
Description Science Committee - Multidisciplinary Project Award
Amount £403,597 (GBP)
Funding ID C368/A22099 
Organisation Cancer Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2016 
End 07/2019
 
Title 3rd gen multiphoton microscope 
Description We have designed and built a 'third generation' multiphoton microscope which enables label-free chemically-specific Coherent Antistokes Raman Scattering (CARS) microscopy and Stimulated Raman Scattering (SRS) over a wider vibrational frequency range and with reduced photodamage compared to previous generation systems. CARS and SRS functionalities are complemented by confocal point and line-scan Raman imaging. The multimodal platform also enables label-free second harmonic generation (SHG) and third-harmonic generation (THG) imaging, as well as background-free sensitive imaging of single small metallic nanoparticles (NPs) via resonant four-wave mixing (FWM) in the same instrument and includes state-of-the art fluorescence modalities, namely two-photon fluorescence (TPF), fluorescence lifetime imaging (FLIM), and fluorescence correlation spectroscopy (FCS). 
Type Of Technology Physical Model/Kit 
Year Produced 2017 
Impact The instrument has already enabled to generate further funding through successful grant proposals. 
URL http://www.cardiff.ac.uk/research-equipment/facilities/view/biophotonics-laboratory
 
Title Heterodyne CARS microscopy 
Description We have developed a novel label-free technique based on chemical imaging via coherent anti-Stokes Raman scattering microscopy using a dual-polarization balanced heterodyne detection in epi-geometry (eH-CARS). This technique provides a unique combination of background-free chemically-specific image contrast for nanoparticles and interfaces, shot-noise limited detection, and phase sensitivity. 
Type Of Technology New/Improved Technique/Technology 
Year Produced 2015 
Impact This new technique could prove instrumental to investigate the chemical composition of thin films with high spatial resolution, topographic sensitivity, and label-free. It could be a powerful tool to study single lipid bilayers, including model and cellular membranes, as well as single nanoparticles. 
 
Title Single particle tracking via four-wave-mixing optical vortex interferometry 
Description We have developed and demonstrated a new four-wave-mixing interferometry technique, whereby the position of a single nonfluorescing gold nanoparticle is determined with nanometric precision in 3D from rapid single-point measurements at 1-ms acquisition time by exploiting optical vortices. The technique is also uniquely sensitive to particle asymmetries of only 0.5% ellipticity, corresponding to a single atomic layer of gold, as well as particle orientation. This method opens new ways of unraveling single-particle trafficking within complex 3D architectures. 
Type Of Technology New/Improved Technique/Technology 
Year Produced 2015 
Impact Ultimately, this new method paves the way towards a new form of single-particle tracking, where not only the nanoparticle position but also its asymmetry, orientation, and chirality are detected with submillisecond time resolution, revealing much more information about the nanoparticle and its complex dynamics (e.g., hindered rotation) while moving and interacting within a disordered 3D environment. As such it can have many applications in physical science systems (e.g. diffusion in complex liquids and gels, liquid crystals) and in the life sciences (single-molecule intra-cellular trafficking). 
 
Description BioNanoPhotonics Symposium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact This is a scientific symposium that I organise every two years, with international invited speakers and participation from industry and postgraduate students. We started having 40 delegates in 2011 and reached 80 delegates in 2015. The event has sparked many scientific discussions and further collaboration with industry among the delegates.
Year(s) Of Engagement Activity 2011,2013,2015,2017
URL http://www.bionanophoto.org.uk/
 
Description Petrus PhD Conference 2015, France 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr S Tripathy, Senior Lecturer, Geoenvironmental Research Centre delivered a special Lecture - Modelling the coupled physical and chemical processes in engineered barrier systems in relation to safety analysis/assessment, at Pertus PhD Conference at Nancy during 22nd - 26th June 2015. Discussions followed by the lecture included topics of significant relevance to advanced testing techniques associated with nuclear waste disposal and implementation.
Year(s) Of Engagement Activity 2015
URL http://petrus2015.strikingly.com/