Developing the NanoKick bioreactor to enable tissue engineered bone graft and use of metabolomics to identify bone specific drug candidates.

Lead Research Organisation: University of Glasgow
Department Name: College of Medical, Veterinary &Life Sci

Abstract

Bone graft is regularly used in surgery (plastics, maxillofacial surgery and orthopaedics); bone is actually the second most grafted tissue after blood. Ideally the surgeon wishes to take bone from one area (donor site) to another area (recipient site) to support the operation they are performing. However, a patient's own donor bone is in short supply and its removal can lead to complications in the donor site. This means the surgeon will often recourse to allograft - decellularised (and thus biologically inferior) - bone from other people. A third, and growing, option is synthetic graft. Synthetic graft can be made from biologically active materials, but is not viable and thus not yet as good as living bone.
Our bioreactor, that supplies nanoscale 'kicks' to cells in culture can be used to convert mesenchymal stem cells (the stem cells of the bone, simple to isolate from a patient's iliac crest or fat tissue) to bone forming osteoblasts. It can achieve this with cells seeded into 3D environments such as gels or potentially synthetic graft materials. This thus allows us to envisage supply of living bone graft derived from a patient's own cells. The ability to supply such materials would provide a new gold standard for bone grafting.
In this project we will thus develop our bioreactor into a flexible platform for study of bone regeneration (which will also be of significant interest to many academic labs in the field) and provision of bone graft.

Further to this vision of tissue engineered bone supply, there is also a big need in Pharma for relevant bone models to reduce use of both standard lab models that are very dissimilar to the in-body environment and animal testing which has large cost and ethical consideration. Our ability to produce 3D bone in the lab simply, reproducibly, at low cost and without need for chemical control of cell phenotype (we will just use the nanokicks) will provide an excellent model for testing of drugs for e.g. osteoporosis, osteogenesis imperfecta and other bone conditions. In this project, we will use our technique to study 3D bone formation in the lab and look at what metabolites, the basic building blocks of life, the cell use as they form bone. We will then identify bioactive metabolites and validate them in our bone mimics.

Finally, we will test to see feasibility of applying nanokicks to humans to help treat e.g. spinal injury, slow bone repair and osteoporosis etc. We will move from mechanical nanokicks to acoustic nanokicks to achieve this.

Planned Impact

Scientists and Healthcare Professionals
We will use open access publishing to disseminate as broadly as possible. This will be of direct interest to the biomaterials, tissue engineering, stem cells, pharma and medical communities.

Laboratories
There are a range of bioreactors available to academic laboratories including mechanical (eg BOSE, EBERS), perfusion (eg BOSE, Minucell) and rotary (eg Synthecon). All have drawbacks for bone production ranging from affordability, sterility, usability and functional outcome. Our system's advantage is that it uses standard cell culture consumables and thus standard protocols. This will clearly open up 3D bone research to many more labs.

Industry
In the first instance our work will be of interest to industrialists working in the mechanotransduction (bioreactor), material science (synthetic bioactive graft) and cell culture plastics manufacture areas. We will develop a bioreactor, consumables for it and try to move to use synthetic tissue engineering scaffolds for our 3D bone graft. Companies working in this area include those with interest in bioreactors (e.g. BOSE, EBERS, Minuth, Synthecon etc), providers of innovative cell cultureware and tissue engineering scaffolds (e.g. BiogelX, Stem Cell Technologies, Porvair and Nunc) and technology developers such as Linn and Physik Instrumente. These companies would have economic benefit from co-developing, licensing and exploiting our bioreactors and consumables for them. Secondly, we will appeal to Pharma with our novel mass spectrometry approaches to drug design (e.g. GSK). Thirdly, we will appeal to companies with an interest in advanced technologies, such as Genzyme and Advanced Tissue Solutions, who will be interested in tissue engineering approaches to bone graft. Finally we will develop an entirely new apparatus for acoustic nanokicking that could be developed to a handheld device for use in clinic. For this, companies with interests in precision signalling will be approached (e.g. Linn).

Patients
Bone graft is the second most transplanted tissue after blood. Autograft harvest from e.g. the iliac crest has a range of associated complications including herniation, instability, nerve damage, infection, deep hematoma requiring surgical intervention and pain [1]. Synthetic bone graft is increasing in use but is biologically inferior and tends to be used with BMP (e.g. Infuse)[2]. BMP has to be used in high doses [3] and this has led to serious respiratory, neurological and inflammatory complications and the issuing of a public health warning by the FDA [4]. Subsequently, reports of the carcinogenic potential of BMP have been noted. The development of tissue engineered bone graft with good volume (cms3) would be a major advantage. All surgeons we have surveyed strongly agree there is urgent unmet need.

Health Service Providers
- Delivery of tissue engineered bone graft would reduce the need for two surgeries. This would represent a saving on surgical time and could be competitively placed compared to e.g. BMP treatment, which can cost up to £3k per patient in non-union fixation [4].
- Delivery of novel drugs for bone conditions. We will identify bioactive metabolites to aid with bone repair and e.g. osteoporosis. Our model could also be used to test 3rd party drugs, conditions such as osteogenesis imperfecta and to advise personal therapies.
- Delivery of novel clinical instruments for bone regeneration i.e. acoustic nanokicking.

Public
Our work will raise interest in science, increasing awareness of the scientific challenges of supporting an aging population. We will engage with the next generation of scientists to inspire them to overcome the burden and limitations of bone grafting and bone regeneration.

1 Arrington 1996. Clin Orthop Relat Res. 329, 300
2 Epstein 2013. Surg Neurol. 4, S343
3 Garrison 2007. NIHR Health Technology Assessment
4 FDA Public Health Notification
5 Dahabreh 2007. JBJ Editorial

Publications

10 25 50
publication icon
Donnelly H (2018) Designing stem cell niches for differentiation and self-renewal. in Journal of the Royal Society, Interface

publication icon
Kareem MM (2019) Hybrid core-shell scaffolds for bone tissue engineering. in Biomedical materials (Bristol, England)

publication icon
Robertson SN (2018) Control of cell behaviour through nanovibrational stimulation: nanokicking. in Philosophical transactions. Series A, Mathematical, physical, and engineering sciences

 
Description We have developed a novel bioreactor that can be used to stimulate MSCs to form tissue engineered 3D bone graft. Further, we are understanding biological mechanism and working towards a new, accoustic bioreactor.
Exploitation Route We are working towards a spin out company formation
Sectors Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description It is early stage, but we are working on spin-out formation. We are currently progressing towards clinical trial (2021) of osteoblsts produced by the bioreactor and also a first veterinary trial in the next few years.
First Year Of Impact 2017
Sector Healthcare
Impact Types Economic

 
Description Biotechnology Young Entrepreneurs Scheme (YES) Competition
Amount £4,000 (GBP)
Organisation University of Glasgow 
Sector Academic/University
Country United Kingdom
Start 09/2018 
End 10/2018
 
Description Directing Chondrogenic Differentiation Through Stimulation with a Nanovibrational Bioreactor
Amount £18,298 (GBP)
Organisation University of Glasgow 
Sector Academic/University
Country United Kingdom
Start 07/2018 
End 02/2019
 
Description Early diagnosis and intervention of osteoporosis using nanovibrational stimulation
Amount £480,000 (GBP)
Funding ID ST/S000852/1 
Organisation Science and Technologies Facilities Council (STFC) 
Sector Academic/University
Country United Kingdom
Start 11/2018 
End 10/2020
 
Description Engineering growth factor microenvironments - a new therapeutic paradigm for regenerative medicine
Amount £3,661,144 (GBP)
Funding ID EP/P001114/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 10/2016 
End 09/2021
 
Description IAA BBSRC
Amount £5,200 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2016 
End 06/2016
 
Description Impact Acceleration Account (IAA)- Consultant to advise on pharmaceutical industry market for Nanokick technology
Amount £20,000 (GBP)
Organisation Science and Technologies Facilities Council (STFC) 
Sector Academic/University
Country United Kingdom
Start 01/2017 
End 02/2017
 
Description Impact Acceleration Account (IAA)- Nanokicking: Expanding the proposition for the Pharma industry
Amount £10,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2018
 
Description The Role of Connexons in Nanovibration-driven Chondrogenic Differentiation of Mesenchymal Stem Cells
Amount £6,980 (GBP)
Organisation Carnegie Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 06/2018 
End 12/2018
 
Description Wellcome Trust ISSF Early Career Researcher Fund
Amount £18,350 (GBP)
Organisation Wellcome Trust 
Department Wellcome Trust Institutional Strategic Support Fund
Sector Charity/Non Profit
Country United Kingdom
Start 03/2018 
End 12/2018
 
Title Polymer surfaces to control growth factor presentation. 
Description We have made a polymeric surface that drives organisation of fibronectin into nanonetworks that bind growth factors at ultra-low dose but with high biological efficacy. 
Type Of Material Technology assay or reagent 
Year Produced 2016 
Provided To Others? Yes  
Impact We have performed a first veterinal trial with the technology that has been very successful. We are now progressing towards human use. 
 
Title Temporal transcriptional pattern in human bone merrow derived mesenchymal stem cell differentiation 
Description Identification of transcription factors and bone marker genes expression pattern through MSCs osteogenic differentiation. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2014 
Provided To Others? Yes  
Impact This transcriptional pattern has been adopted by other research projects on which MSCs osteogenic differentiation is stimulated by physical cues. 
 
Title Use of NanoKick Bioreactor for chondrogenic differentiation of MSCs 
Description Modification of cell culture method to induce chondrogenic differentiation of MSCs when using the NanoKick Bioreactor. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2017 
Provided To Others? No  
Impact Preliminary data obtained further funding from a Wellcome Trust ISSF Early Career Catalyst Fund (~£18300). 
 
Title Control of cell behaviour through nanovibrational stimulation: nanokicking 
Description  
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
 
Description Collaboration for Synthesis of Osteogenic Metabolites and Chemical Derivatives 
Organisation University of Glasgow
Department School of Chemistry
Country United Kingdom 
Sector Academic/University 
PI Contribution Identification of interesting metabolites in osteogenesis and in vitro testing of osteogenic potential
Collaborator Contribution Chemical synthesis of metabolite molecules
Impact We will shortly publish results from this collaboration.
Start Year 2017
 
Description Collaboration with Dr Lee Buttery, University of Nottingham 
Organisation University of Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of a Nanokick bioreactor
Collaborator Contribution Testing of the device and expertise in chemotaxis/osteogenic culture.
Impact Disciplines: Physics, cell engineering
Start Year 2017
 
Description Collaboration with Dr Paul Brennan, University of Edinburgh 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of a Nanokick bioreactor
Collaborator Contribution Device testing and expertise in glialblastoma stem cells.
Impact Disciplines: Neuro oncology, cell engineering
Start Year 2018
 
Description Collaboration with Dr. Manus Biggs in National University of Ireland, Galway to creat Dual Piezoelectric/Nanovibration system for rapid bone regeneration 
Organisation National University of Ireland, Galway
Country Ireland 
Sector Academic/University 
PI Contribution Combining piezoelectric scaffold provided by Dr Manus group with our nanovibrator for MSCs osteogenic stimulation.
Collaborator Contribution Synthesizing piezoelectric scaffold.
Impact The collaboration just started recently. We will publish a paper in near future.
Start Year 2017
 
Description Collaboration with Geistlich 
Organisation Geistlich
Country Switzerland 
Sector Private 
PI Contribution Testing of in vitro cell culture on Geistlich implant materials
Collaborator Contribution Contribution of implant materials from Geistlich
Impact Disciplines involved - Dentistry, cell engineering, materials
Start Year 2018
 
Description Collaboration with Prof Bo Su, University of Bristol 
Organisation University of Bristol
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of a Nanokick bioreactor
Collaborator Contribution Testing of the device and expertise in bacterial culture.
Impact Disciplines: Cell engineering, biomaterials
Start Year 2018
 
Description Collaboration with Prof Godfrey Smith, University of Glasgow 
Organisation University of Glasgow
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of a Nanokick bioreactor
Collaborator Contribution Testing of the device and experience with cardiomyocyte culture.
Impact Disciplines: cell engineering, cardio physiology, physics.
Start Year 2018
 
Description Collaboration with Prof Stuart Reid, University of Strathclyde 
Organisation University of Strathclyde
Country United Kingdom 
Sector Academic/University 
PI Contribution Together, we have designed a new bioreactor and consumables for it.
Collaborator Contribution They design and build the bioreactors.
Impact THis is a long standing collaboration in which we designed and build the nanovibrational bioreactor (originally Nanoforce, now Nanokick). We have worked togther, produced patents and publications as well as securing further funding.
Start Year 2011
 
Description Collaboration with the Scottish National Blood Transfusion Service 
Organisation NHS National Services Scotland (NSS)
Department Scottish National Blood Transfusion Service
Country United Kingdom 
Sector Public 
PI Contribution Provision of technology for osteogenic cell culture.
Collaborator Contribution Expertise in GMP manufacture of cellular therapies.
Impact Disciplines: GMP manufacture, cell engineering, physics
Start Year 2016
 
Description Souhtampton Collaboration 
Organisation University of Southampton
Department Bone and Joint Research Group
Country United Kingdom 
Sector Academic/University 
PI Contribution This is a long term collaboration where cells, materials and expertise are shared to drive research
Collaborator Contribution This is a long term collaboration where cells, materials and expertise are shared to drive research
Impact A large number of papers, grants and IP.
 
Title Mechanical Bioreactor 
Description Design of a novel nanovibrational bioreactor for bone graft production 
IP Reference WO2017029393A1 
Protection Patent application published
Year Protection Granted 2017
Licensed No
Impact None yet
 
Description FEBS Workshop Biological Surfaces and Interfaces: Interface Dynamics, 2nd-7th July 2017, Sant Feliu de Guixols, Catalonia, Spain 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Mr W. Orapiriyakul had a Poster presentation at FEBS Workshop Biological Surfaces and Interfaces: Interface Dynamics, 2nd-7th July 2017, Sant Feliu de Guixols, Catalonia, Spain
Year(s) Of Engagement Activity 2017
 
Description Glasgow Orthopaedic Research Initiative (GLORI 2018), 23d February 2018, Glasgow, UK. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Mr W. Orapiriyakul had a Poster presentation at Glasgow Orthopaedic Research Initiative (GLORI 2018), 23th Febuary 2018, Glasgow, UK.
Year(s) Of Engagement Activity 2018
 
Description 28th Annual Conference of the European Society for Biomaterials (ESB) 4-8th September 2017, Athens, Greece. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Mr W. Orapiriyakul: Oral presentation at 28th Annual Conference of the European Society for Biomaterials (ESB) 4-8th September 2017, Athens, Greece.
Year(s) Of Engagement Activity 2017
 
Description 29th European Conference on Biomaterials (ESB 2018), 9-13th September 2018, Maastricht, Netherlands. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Mr W. Orapiriyakul had a Poster presentation in 29th European Conference on Biomaterials (ESB 2018), 9-13th September 2018, Maastricht, Netherlands.
Year(s) Of Engagement Activity 2018
 
Description 5th TERMIS World Congress 2018, 4-7th September 2018, Kyoto, Japan. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact mr W.Orapiriyakul had a Poster presentation in 5th TERMIS World Congress 2018, 4-7th September 2018, Kyoto, Japan.
Year(s) Of Engagement Activity 2018
 
Description BCRT annual conference July 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Dr Tsimbouri was asked to have a public exhibition at the BCRT annual conference in Birmingam international in July 2017,about the Nanokicking Bioreactor and its potential uses in bone regeneration.
Year(s) Of Engagement Activity 2017
URL https://www.bcrt.org.uk/get-involved/events-and-challenges/bone-cancer-conference-2017
 
Description BLOG CeMi: NANOKICKING STEM CELLS TO GROW NEW BONE 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr Monica P. Tsimbouri wrote a blog about the people, work and challenges involved for the recent paper
Tsimbouri, Childs et al. Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nat. Biomed. Eng. http://doi.org/10.1038/s41551-017-0127-4 (2017).
Year(s) Of Engagement Activity 2017
URL https://www.gla.ac.uk/research/az/thecemi/blog/nanokickingstemcellstogrownewbone/
 
Description BORS 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact We organised the British Orthopedic Society annual conference in Glasgow. The conference sparked discussion after every talk and new collaborations were initiated between research and clinical groups.
Year(s) Of Engagement Activity 2016
 
Description Biomaterials seminar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Mr W Orapiriyakul gave a talk at the Biomaterials seminars session on 13/11/18
Year(s) Of Engagement Activity 2018
 
Description Biomaterials seminar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Wich Orapiriyakul, Oral presentation, Biomaterial seminar, University of Glasgow, 1st Nov, 2016
Year(s) Of Engagement Activity 2016
 
Description Conference presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Wich Orapiriyakul Poster presentation in 10th UK Mesenchymal Stem Cell Meeting, 5th December 2016 York, UK.
Year(s) Of Engagement Activity 2017
 
Description Conference presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Conference presentation by Tom Hodgkinson at European Society for Biomaterials, Maastricht
Year(s) Of Engagement Activity 2018
 
Description Conference presentation 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Wich Orapiriyakul Poster presentation at FEBS Workshop Biological Surfaces and Interfaces: Interface Dynamics, 2nd-7th July 2017, Sant Feliu de Guixols, Catalonia, Spain
Year(s) Of Engagement Activity 2017
URL https://mimeresearch.com/biointerfaces2017/
 
Description Conference presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Identification and in vitro screening of osteogenic metabolites through supplement-free nanovibration-driven mesenchymal stem cell differentiation (2018) Tom Hodgkinson, Karl Burgess, David France, Paul Campsie, Shaun Robertson, David Phillips, Peter Childs, Richard Oreffo, Stuart Reid, Manuel Salmeron-Sanchez, Matthew Dalby - TCES 2018, Keele, UK.
Year(s) Of Engagement Activity 2018
 
Description Conference presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Identification and in vitro screening of osteogenic metabolites through supplement-free nanovibration-driven mesenchymal stem cell differentiation (2018) Tom Hodgkinson, Karl Burgess, David France, Paul Campsie, Shaun Robertson, David Phillips, Peter Childs, Richard Oreffo, Stuart Reid, Manuel Salmeron-Sanchez, Matthew Dalby - Matrix Biology Europe, Manchester, UK.
Year(s) Of Engagement Activity 2018
 
Description Conference presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Wich Orapiriyakul Poster presentation, British Orthopaedic research Society (BORS), UK, 5-6th September 2016, Glasgow, UK
Year(s) Of Engagement Activity 2016
URL https://www.uofgcce.org/bors-2016
 
Description Conference presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Identification and in vitro screening of osteogenic metabolites through supplement-free nanovibration-driven mesenchymal stem cell differentiation (2018) Tom Hodgkinson, Karl Burgess, David France, Paul Campsie, Shaun Robertson, David Phillips, Peter Childs, Richard Oreffo, Stuart Reid, Manuel Salmeron-Sanchez, Matthew Dalby - Matrix Biology Europe, Manchester, UK.
Year(s) Of Engagement Activity 2018
 
Description Conference presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Third sector organisations
Results and Impact Identification and in vitro screening of osteogenic metabolites through supplement-free nanovibration-driven mesenchymal stem cell differentiation (2018) Tom Hodgkinson, Karl Burgess, David France, Paul Campsie, Shaun Robertson, David Phillips, Peter Childs, Richard Oreffo, Stuart Reid, Manuel Salmeron-Sanchez, Matthew Dalby - GLORI, Glasgow UK.
Year(s) Of Engagement Activity 2018
 
Description GLORI at the CeMi 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Tom Hodgkinson, Talk
"Identification and in vitro screening of osteogenic metabolites through supplement-free
nanovibration-driven mesenchymal stem cell differentiation"
Glasgow Orthopedic Research Initiative 2018
23rd November 2018
Year(s) Of Engagement Activity 2018
 
Description GLORI at the CeMi 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Mr Ian Kennedy presented a talk on
"Nanoscale vibrations to modulate osteogenesis"
Glasgow Orthopedic Research Initiative 2018
23rd November 2018
Year(s) Of Engagement Activity 2018
 
Description GLORI conference organisation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Planning and Organisation of Dr Monica P Tsimbouri the scientific mentor of the Glasgow Orthopaedic Research Initiative (GLORI). GLORI has been established to encourage collaboration between the basic sciences, applied sciences, engineering and clinic. This has the aim of bringing the latest ideas in basic materials research into use to deliver the next-generation of orthopaedic care. It combines expertise from orthopaedic surgeons, biologists, engineers and chemists not only from Glasgow and Strathclyde Universities but the rest of Scotland too and also with the view to expand to the rest of the UK.
Year(s) Of Engagement Activity 2018
 
Description GLORI-2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact National orthopedic conference in Glasgow where about 60 audience attended.
Year(s) Of Engagement Activity 2018
 
Description Glori2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Submitted a research abstract, stimulating discussion and chaired a conference session on bone regeneration strategies
Year(s) Of Engagement Activity 2018
 
Description IKEA at home with life sciences 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Dr Monica P. Tsimbouri
participated at the
IKEA at Home with Life Sciences
A team of students, researchers and support staff represented the Institute at IKEA on Saturday 24th November 2018 as part of the MVLS at IKEA event.
Year(s) Of Engagement Activity 2018
URL https://www.gla.ac.uk/researchinstitutes/biology/newsevents/headline_581051_en.html?utm_medium=email...
 
Description International Conference presentation 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Wich Orapiriyakul did a Podium presentation at 28th Annual Conference of the European Society for Biomaterials (ESB) 4-8th September 2017, Athens, Greece
Year(s) Of Engagement Activity 2017
URL http://ESB2017.org
 
Description Invited talk at BCRT Scotland day May 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact This was an invited talk for Dr Monica P. Tsimbouri by BCRT in Perth May 16th 2017. The presentation was intended for clinical partitioners as well as patients and carers affected by bone cancers. There was a lot of interest and discussions after the talk and Dr Tsimbouri was asked to give a further presenation in the future.
Year(s) Of Engagement Activity 2017
URL https://www.bcrt.org.uk/news/2017/may/our-first-scottish-patient-and-carer-day
 
Description Local Talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Wich Orapiriyakul Oral presentation, GLORI 2016, University of Glasgow, 15th Dec 2016
Year(s) Of Engagement Activity 2016
 
Description Media presentation 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Radio interview for the Today Programme, Radio Scotland and BBC World Service
Year(s) Of Engagement Activity 2016
 
Description NEB community: the story behind the paper 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr P.M. Tsimbouri and Dr P. Childs were asked by the NEB community to write a story behind their recent paper
Tsimbouri, Childs et al. Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nat. Biomed. Eng. http://doi.org/10.1038/s41551-017-0127-4 (2017).

Story title:
Growing bone using nanovibration:
A reproducible method for mechanically driving stem-cell osteogenesis in 3D culture.
Year(s) Of Engagement Activity 2017
URL http://go.nature.com/2xVgSNN
 
Description Nanotoxicology Meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Invited talk for the Naotoxicology Society
Year(s) Of Engagement Activity 2016
 
Description Oral Presentation at Biomaterials Seminar, University of Glasgow 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Presented research on nano vibrational differentiation of mesenchymal stem cells as part of the biomaterials seminar series at the University of Glasgow
Year(s) Of Engagement Activity 2018
 
Description Poster presentation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Wich Orapiriyakul presented a poster at GLORI2018
Year(s) Of Engagement Activity 2018
 
Description Press release to announce Find a Better Way funding 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Press release to announce the award of our Find a Better Way Project
Year(s) Of Engagement Activity 2016
 
Description Programme grant announcement 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Press release to announce Glasgow EPSRC Programme grant
Year(s) Of Engagement Activity 2016
 
Description Public outreach-Royal Society of London Summer Exhibition 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Nanokicking" Royal Society Science summer exhibition in London 2016.
We promoted our Nanokicing research to the public and explaining the potential of nanokicking in
bone regeneration and also its use as a tool in cancer research.
Year(s) Of Engagement Activity 2016
 
Description Royal Society Summer Exhibition 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact We presented our growth factor based bone regeneration technology at the Exhibition
Year(s) Of Engagement Activity 2018
 
Description Royal Society Summer Exhibition Press Release 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Press release from the Royal Society about our nanokick exhibit
Year(s) Of Engagement Activity 2016
 
Description Royal Society of Chemistry Biomaterials Meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Conference presentation
Year(s) Of Engagement Activity 2017
 
Description SSN invited talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Dr P. M. Tsimbouri was invited to present the use of nanotopography on Titanium orthopedic implants and the use of Nanokicking Bioreactor for bone regeneration and also as a tool for the descovery of metabolic targets in bone cancer for pharma.
Year(s) Of Engagement Activity 2016
 
Description Scottish Gocernment Outreach Event Edinburgh 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Outreach event to mark the Scottish Governments science policy launch
Year(s) Of Engagement Activity 2018
 
Description Scottish Metabolomics Network 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Invited talk
Year(s) Of Engagement Activity 2016
 
Description Talk at GLORI2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Dr Monica P Tsimbouri delivered a talk on behalf of Prof Dalby at GLORI2018
Year(s) Of Engagement Activity 2018
 
Description The University Network ((TUN) 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr Monica P. Tsimbouri was invited by the The University Network ((TUN), a global media company for college students available in 108 languages) to write an article about the nanokicking project. (https://www.tun.com/blog/nanokicking-technology-bone-growth-development/)
Year(s) Of Engagement Activity 2017
URL https://www.tun.com/blog/nanokicking-technology-bone-growth-development
 
Description school workshop: Bones and bone cells, 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Dr P.M. Tsimbouri has organised a full day workshop for P5,P6 and P7 school pupils at the Milngavie primary school.
The workshop involves presentation slides and Q/A session with the children about the skeleton, bones and bone cells. This followed by hands on experience looking at fixed cells using bright field microscopes. The children were given colour-in pages and also word searches to take home.
Year(s) Of Engagement Activity 2015,2017