A smart, multi-purpose technology for diagnostics, analytics and drug delivery
Lead Research Organisation:
University of Birmingham
Department Name: Chemical Engineering
Abstract
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Organisations
Publications
| Description | Our vision is to establish a smart multi-purpose exosome technology platform that radically advances personalised medicine across diagnostics, analytics and drug delivery. In 30 years' time this technology platform will be widely used in healthcare to diagnose and treat disease with high fidelity using bespoke formulations. In order to advance this vision, phase 1 development studies have been addressing engineering challenges across three inter-connected themes on bioprocess strategies and sensor innovations for generating and detecting exosomes at different orders of sensitivity, examining feasibility ahead of phase 2. So far we have been advancing the creation of a biosensor platform that detects exosome analytes from ATMP cell cultures with high specificity and sensitivity. Baseline mesenchymal stem cell cultures were established in 100mL bioreactors on polystyrene microcarriers. Exosomes were harvested from culture media throughout the culture at the point of media exchange by removing 50% of the culture media and replenishing with fresh media. We used monoclonal antibodies to selectively pull out the target exosomes and spectroscopically detect changes in signal. Raman spectroscopy and microscopy imaging for single exosomes was used to concurrently characterize and monitor physical and chemical properties of exosomes. Bioprocess studies undertaken produced exosomes from human mesenchymal stem cells at pilot scale using scalable bioreactor technologies that are amenable to tech transfer into a GMP facility. |
| Exploitation Route | Phase 1 development studies revealed insights that have allowed us to revise our approach and team in phase 2, to leverage state of the art approaches to bioprocessing and sensor innovations for generating and detecting exosomes at different orders of sensitivity. |
| Sectors | Digital/Communication/Information Technologies (including Software),Healthcare |
| Description | MacRobert Award Panel |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | This panel discussion included former winners of the prestigious MacRobert Award for UK engineering innovation, looking at how we can create a hospitable environment for engineering and what some of the next revolutionary developments might be. |
| Year(s) Of Engagement Activity | 2022 |