Role of fibrillin microfibrils in elastic fibre formation

Lead Research Organisation: University of Manchester
Department Name: Medical and Human Sciences

Abstract

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Publications

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Baldock C (2006) Nanostructure of fibrillin-1 reveals compact conformation of EGF arrays and mechanism for extensibility. in Proceedings of the National Academy of Sciences of the United States of America

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Kielty CM (2006) Elastic fibres in health and disease. in Expert reviews in molecular medicine

 
Title Cell culture models for elastic fibre biology 
Description We have a number of cell culture models to study elastic fibre deposition; they include primary human dermal fibroblasts and the ARPE-19 cell line; we have also developed robust siRNA and shRNA approaches for knockdowns, and retroviral knock-in approaches in these cultures. 
Type Of Material Cell line 
Provided To Others? No  
Impact These cell lines are enabling us (a) to determine the molecular mechanisms underlying elastic fibre formation. 
 
Title Recombinant elastic fibre proteins 
Description We have developed a large panel of recombinant human elastic fibre proteins (including fibrillin-1, fibrillin-2, MAGP-1, fibulins 3-5, LTBPs 1 and 3, periostin). 
Type Of Material Technology assay or reagent 
Year Produced 2008 
Provided To Others? Yes  
Impact Publications describing molecular interactions in elastic fibre assembly, and structural analyses of a number of these fragments by small-angle X-ray scattering and EM approaches (these structural studies were led by Dr C Baldock). 
 
Description Collaboration with Dr Clair Baldock (Manchester) 
Organisation Wellcome Trust
Department Wellcome Trust Centre for Cell-Matrix Research
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution My contribution is the research topic and the major research input into understanding how elastic fibres assemble. Dr Baldock contributes through her structural biology expertise to my research on this topic.
Collaborator Contribution Structural biology expertise.
Impact A number of research papers, as outlined in this e-Val report. 19570982 19617354 17166706 16905551 16880403
Start Year 2006