structural mri for rhesus monkeys
Lead Research Organisation:
University of Oxford
Department Name: Experimental Psychology
Abstract
Direct public dissemination of this work is limited by the reluctance of scientists to expose themselves to antivivisectionist actions. However, our work often appears in secondary sources such as television and popular science magazines.
Technical Summary
We will use structural magnetic-resonance imaging (MRI) to scan the brains of macaque monkeys. The scans will enable us to accurately place recording electrodes in cortical and subcortical targets in electrophysiological experiments. In ablation experiments, the scans will enable us to place heat lesions or neurotoxic injections accurately in subcortical targets. This facility will allow us to perform experiments that cannot be performed without the benefit of MRI scanning.
Publications
Ainsworth M
(2018)
Functional reorganisation and recovery following cortical lesions: A preliminary study in macaque monkeys.
in Neuropsychologia
Ainsworth M
(2021)
Viewing ambiguous social interactions increases functional connectivity between frontal and temporal nodes of the social brain.
in The Journal of neuroscience : the official journal of the Society for Neuroscience
Akaishi R
(2016)
Neural Mechanisms of Credit Assignment in a Multicue Environment
in Journal of Neuroscience
Apps MA
(2016)
The Anterior Cingulate Gyrus and Social Cognition: Tracking the Motivation of Others.
in Neuron
Folloni D
(2021)
Ultrasound modulation of macaque prefrontal cortex selectively alters credit assignment-related activity and behavior.
in Science advances
Fouragnan E
(2019)
The macaque anterior cingulate cortex translates counterfactual choice value into actual behavioral change
in Nature Neuroscience
| Description | Frontal cortical mechanisms and interactions during learning and decision making |
| Amount | £2,500,000 (GBP) |
| Funding ID | G0902373 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2011 |
| End | 04/2016 |
| Description | Integrated neural networks of the brain |
| Amount | £4,955,047 (GBP) |
| Funding ID | Strategic Award PI Professor John Duncan |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2014 |
| End | 04/2020 |
| Description | MRC Centenary Award |
| Amount | £99,000 (GBP) |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2012 |
| End | 04/2015 |
| Description | Neuromodulatory-prefrontal interactions in primates |
| Amount | £4,429,363 (GBP) |
| Funding ID | BB/W003392/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 02/2022 |
| End | 01/2027 |
| Description | Senior Research Fellowship in Basic Biomedical Sciences |
| Amount | £2,307,000 (GBP) |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 03/2016 |
| End | 06/2022 |
| Title | MRI scanning non-human primates |
| Description | database of MRI scans (structural, functional, diffusion tensor scans) in monkeys including neurologically normal controls and lesion groups to compare. |
| Type Of Material | Biological samples |
| Year Produced | 2010 |
| Provided To Others? | Yes |
| Impact | Several publications using the control and lesion group scans development by MR imaging group at FMRIB of further FSL software packages to perform monkey brain extraction (BET) from the MR images M.P. Milham, et al. An Open Resource for Non-human Primate Imaging. Neuron, 100 (2018), pp. 61-74.e2 https://fcon_1000.projects.nitrc.org/indi/indiPRIME.html |
| Title | resting state fMRI data bases |
| Description | macaque resting state functional magnetic resonance imaging data sets |
| Type Of Material | Biological samples |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | papers published by other research groups: Kumar V, Croxson PL, Simonyan K (2016) Structural Organization of the Laryngeal Motor Cortical Network and Its Implication for Evolution of Speech Production. J Neurosci 36:4170-4181. Mitchell DJ, Bell AH, Buckley MJ, Mitchell AS, Sallet J, Duncan J (2016) A Putative Multiple-Demand System in the Macaque Brain. J Neurosci 36:8574-8585. |
| Description | MRI collaboration to set up monkey scanning |
| Organisation | John Radcliffe Hospital |
| Department | Centre for Functional MRI of the Brain (fMRI) |
| Country | United Kingdom |
| Sector | Hospitals |
| PI Contribution | I hold and manage one of the Home Office project licences to carry out this research I've contributed anaesthesia knowledge and management, I've contributed to the set up of MRI scanning in anaesthetised monkeys I've provided anaesthesia for my own and other researchers animals in experiments, I've provided anaesthesia equipment for use in animals under general anaesthesia, I've learnt to operate the MR scanner for MR imaging of monkeys for my own work and other researchers I've developed MR knowledge for diagnositc/clinical purposes related to postoperative neurosurgery complications for monkeys |
| Collaborator Contribution | Partners have contributed to the overall setup of MR scanning in monkeys. Write sequences and continue to optimise these for experiments. provide Q&A support for the scanner image quality provide advice on data storage and management Assist with ideas for analysis of monkey MR imaging and provide MR physics and MR image analysis courses Provide monkey analysis packages via FSL updates Provide overall ongoing support for the MR imaging work Provide scanner operator training and support provide information and training for safety of scanner use and run annual safety courses |
| Impact | outputs for me include training to be scanner operator learning imaging analysis techniques for monkey imaging data received training on how to design MR imaging experiments for monkeys and humans I've developed expertise in using software packages to help with image analysis including FSL, MATLAB, SPM8 I've collected MR scans of my monkeys brains pre-operation and post-operation to document the structural, functional and connectivity changes in their brains as a consequence of the lesions gained knowledge for MR use in diagnosis of complications related to neurosurgery of my monkeys publications listed in relevant section |
| Start Year | 2009 |
| Description | MRI collaboration to set up monkey scanning |
| Organisation | University of Oxford |
| Department | Department of Experimental Psychology |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I hold and manage one of the Home Office project licences to carry out this research I've contributed anaesthesia knowledge and management, I've contributed to the set up of MRI scanning in anaesthetised monkeys I've provided anaesthesia for my own and other researchers animals in experiments, I've provided anaesthesia equipment for use in animals under general anaesthesia, I've learnt to operate the MR scanner for MR imaging of monkeys for my own work and other researchers I've developed MR knowledge for diagnositc/clinical purposes related to postoperative neurosurgery complications for monkeys |
| Collaborator Contribution | Partners have contributed to the overall setup of MR scanning in monkeys. Write sequences and continue to optimise these for experiments. provide Q&A support for the scanner image quality provide advice on data storage and management Assist with ideas for analysis of monkey MR imaging and provide MR physics and MR image analysis courses Provide monkey analysis packages via FSL updates Provide overall ongoing support for the MR imaging work Provide scanner operator training and support provide information and training for safety of scanner use and run annual safety courses |
| Impact | outputs for me include training to be scanner operator learning imaging analysis techniques for monkey imaging data received training on how to design MR imaging experiments for monkeys and humans I've developed expertise in using software packages to help with image analysis including FSL, MATLAB, SPM8 I've collected MR scans of my monkeys brains pre-operation and post-operation to document the structural, functional and connectivity changes in their brains as a consequence of the lesions gained knowledge for MR use in diagnosis of complications related to neurosurgery of my monkeys publications listed in relevant section |
| Start Year | 2009 |
| Description | Neuromodulatory-prefrontal interactions in primates |
| Organisation | University of Oxford |
| Department | Department of Experimental Psychology |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Principal Investigator on BBSRC sLoLa grant |
| Collaborator Contribution | co-applicant on BBSRC sLoLa grant |
| Impact | A new award for investigating brain function |
| Start Year | 2022 |
| Description | Contributor to Lab Animal Tour |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | A virtual visit to the Oxford lab animal facilityWelcome to Oxford University's primate research facility. This facility has been specially designed to accommodate behavioural neuroscience research involving Rhesus Macaque (Macaca mulatta) monkeys. Macaques are socially and cognitively complex animals, characteristics that make them ideal subjects for our behavioural neuroscience studies. Because of these characteristics it is really important that we provide high standards of care. Our species-specific environmental enrichment program and reward-based training strategies are key to ensuring the animals are appropriately acclimatised to the staff, the laboratory environment and the research procedures. During this virtual tour you will have an opportunity to meet the staff and animals, to see the facilities and to learn about some of the studies that we carry out. |
| Year(s) Of Engagement Activity | 2017,2018 |
| URL | http://www.labanimaltour.org |
| Description | UAR Lab animal tour |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | We contributed to Understanding Animals Research videoing of our labs and primates. Creating a virtual lab tour that is freely available on the internet. http://www.labanimaltour.org/ . We won the award for Public Engagement for promoting our research and welfare in December 2017. The project was recently featured in The Scientist (2020), highlighting the significance of such transparency efforts and their long-lasting impact. https://www.the-scientist.com/news-opinion/virtual-lab-tours-for-recruitment-and-outreach-67633?_ga=2.162167548.1571827230.1592989612-318662186.1591107018&mc_cid=ff433471c4&mc_eid=d4f4daa98d) |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.labanimaltour.org/ |
| Description | Understanding Animal Research Lab Tour |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Understanding Animal Research lab tours provides an easily accessible web resource that allows members of the general public to visit and navigate through a primate neuroscience research facility |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.labanimaltour.org/ |
| Description | interview for national news |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Intereviews in national newspapers and scientific journals relating to ultrasound stimulation experiments (eg http://blog.pnas.org/2019/03/journal-club-low-intensity-focused-ultrasound-shows-promise-as-tool-to-probe-deep-brain-function/) |
| Year(s) Of Engagement Activity | 2019 |
| Description | interviews for newspapers and radio broadcasts |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | The first author of a scientific study, Dr Jerome Sallet, spoke to journalists in the UK and abroad, working on newspapers and on radio, about the results he obtained with the MRI scanner funded by the grant. Several items appeared in radio news and in newspapers in the UK and abroad. |
| Year(s) Of Engagement Activity | 2011 |
| Description | non-scientific conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Primary Audience | Policymakers/politicians |
| Results and Impact | The grant supported the purchase of an MRI scanner. One of the applicants, M Rushworth, has presented work undertaken on this scanner at a Skoll Foundation meeting show-casing scientific research on decision making and social cognition and attempting to social entrepreneurs interested in social influence and change. Organizer reported that the audience had found the event interesting |
| Year(s) Of Engagement Activity | 2011 |