Non-invasive monitoring of the effect of biologically targeted anticancer drugs by Raman spectroscopy

Lead Research Organisation: University of Nottingham
Department Name: Sch of Physics & Astronomy

Abstract

Conventional cancer drugs are toxic to both tumour and other normal tissue and so are often associated with significant side effects. Recently, novel drugs which selectively target tumours have been developed. For such drugs (biologically targeted anticancer drugs), the clinical effects are observed at doses below the toxicity levels. Therefore, the side-effects are significantly reduced compared to conventional chemotherapy.
A major challenge in using these targeted drugs however is determining the right dose to use for optimal clincial effect. Since certain molecules targeted by the drugs are also present in skin, we propose to monitor the molecular changes in the skin to assess the efficacy of these novel drugs. We will use a non-invasive optical method (Raman spectroscopy) to detect molecular changes is the skin of patients without need of biospy.If successful this would be a very useful method in the development and clinical use of such drugs.

Technical Summary

The aim of the proposed research is to develop a non-invasive method to monitor the effect of anticancer drugs based on tyrosine kinase inhibitors (TKIs) by using changes in skin as a surrogate end point. We will investigate whether Raman micro-spectroscopy can be used to quantify the biochemical changes in patients? skin related to the effect of TKI-based drugs without need of biopsies or other invasive procedures. If successful, the method will offer enormous advantages over current methods as it is non-invasive, rapid, and objective.

Publications

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publication icon
Verrier S (2011) Raman micro-spectroscopy as a non-invasive cell viability test. in Methods in molecular biology (Clifton, N.J.)

 
Description BBSRC BRIC grant (Non-invasive biophotonics tool for phenotypic identification of pluripotent stem cells and their progeny)
Amount £540,000 (GBP)
Funding ID BB/G010285/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2009 
End 12/2011
 
Description Diagnosis of tumours during tissue conserving surgery by multimodal spectral imaging
Amount £1,394,000 (GBP)
Funding ID EP/L025620/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 10/2014 
End 09/2019
 
Description Invention for Innovation Stream 2.1II-AR-01209-10012 Raman spectral imaging for automated Mohs Micrographic surgery of high-risk Basal Cell Carcinoma
Amount £688,535 (GBP)
Organisation National Institute for Health Research 
Department NIHR i4i Invention for Innovation (i4i) Programme
Sector Public
Country United Kingdom
Start 05/2010 
End 06/2013
 
Description NIHR NEAT
Amount £65,000 (GBP)
Organisation National Institute for Health Research 
Sector Public
Country United Kingdom
Start 01/2008 
End 03/2009
 
Description Raman spectral imaging for automated Mohs Micrographic surgery of high-risk Basal Cell Carcinoma
Amount £665,143 (GBP)
Funding ID II-LA-0813-20001 
Organisation National Institute for Health Research 
Sector Public
Country United Kingdom
Start 07/2014 
End 06/2016
 
Title Raman spectroscopy imaging 
Description label free imaging of live cells, allows time-course experiments on live cells in-vitro 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact new grants, patent applications, publications. 
 
Description Antimicrobial resistance 
Organisation University of Nottingham
Department Centre for Biomolecular Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution The instrumentation developed during the grant allowed us to develop instrumentation to investigate now individual bacteria and biofilms.
Collaborator Contribution Prof Williams' research on bacterial virulence factors and their control systems has enabled identification of molecular 'weak points' in bacteria and discovery of novel compounds active against such targets for novel antibacterial drugs. In order to gain new insight into the biophysics of quorum sensing signal and inhibitor molecules in biofilms, we will use confocal Raman microscopy, which is a non-invasive and chemically specific label-free imaging technique with high spatial resolution, which has been developed extensively in Prof Notingher's group, starting with this grant.
Impact None so far.
Start Year 2017
 
Description Imaging Basal cell carcinoma by Raman spectroscopy 
Organisation Nottingham University Hospitals NHS Trust
Department Nottingham University Hospitals Charity
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Research
Collaborator Contribution Established new partnership for research support and public involvement. Submitted new proposal to EPSRC Healthcare Partnership
Impact Patent application, paper PMID: 19895133
Start Year 2007
 
Description Spectral Imaging of Basal Cell Carcinoma 
Organisation Nottingham University Hospitals NHS Trust
Country United Kingdom 
Sector Public 
PI Contribution joint research project
Collaborator Contribution Expertise in dermatology and Mohs Micrographic Surgery
Impact PMID: 19895133
Start Year 2007
 
Description Optical sensing IOP 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Primary Audience Participants in your research and patient groups
Results and Impact 20 minutes oral presentation

collaborations
Year(s) Of Engagement Activity 2008
 
Description School Kettering 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Primary Audience Schools
Results and Impact Presentation on applications of lasers in medicine

science engagements to school children
Year(s) Of Engagement Activity 2008
 
Description Xth World Congress on Biosensors 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Primary Audience Participants in your research and patient groups
Results and Impact 15 minutes oral presentation

new collaborations
Year(s) Of Engagement Activity 2008