Understanding the genetic controls of resolution of neutrophilic inflammation in zebrafish.

Lead Research Organisation: University of Sheffield
Department Name: Biomedical Science

Abstract

Aside from cancer, the most likely causes of death and disability for an aging population are chronic inflammatory diseases including emphysema (COPD), heart disease, arthritis, and many types of kidney failure. Many doctors (and patients) feel that current best treatment for such conditions is not good enough, in part due to our poor understanding of how inflammation usually gets better. It is thought that inflammation gets better because the neutrophils (the white blood cells causing tissue damage in inflammation) turn off, and declare they are ready to be removed. This occurs by a process called programmed cell death or apoptosis. However, what actually triggers this cell suicide process is not known, although it appears to be controlled by genes within the neutrophil. It is hard to identify these important genes in humans, because the cells involved do not survive long enough outside the body to permit manipulation of their genes. My previous research has indicated that cell surface death receptors and the things they interact with may be important, but there are no ways of testing this by deleting all the things we think are important from cells and seeing if inflammation still resolves. In addition, there are at present no model systems where we can search for the genes involved in an unbiased way, (i.e. without trying to guess the answers first). We can answer both of these questions using zebrafish. We will model inflammation in zebrafish, test ideas about how death receptors lead to the resolution of inflammation, and search in an unbiased way for genes important in causing the resolution of inflammation. Understanding how the body causes inflammation to stop might help us understand why sometimes it doesn‘t, and might show us new ways of speeding the recovery of inflamed organs.

Technical Summary

Inflammatory diseases such as emphysema, asthma, heart disease and arthritis cause much illness in the developed world. We have little understanding of how the severe inflammation associated with something like pneumonia can completely resolve, while other sorts of inflammation persist with associated tissue damage. In fact, we know little of the processes that cause resolution of inflammation in any setting. There is considerable evidence that the main inflammatory cell, the neutrophil, turns itself off by a process called apoptosis (=programmed cell death), and is then recognised by macrophages and then removed. What triggers this process in neutrophils is not known. If we could find this out, we would be a step closer to understanding inflammatory disease, and might have new ideas about the sorts of treatments that might be successful. These things are not possible to study in human cells, and I aim to set up a model system in which the genes controlling resolution of inflammation can be identified. Zebrafish are a good model, since their genes are easily manipulated, and their transparency leads to easy visualisation of neutrophils during development.
I will set up a model of inflammation in zebrafish embryos, in which neutrophils can be visualised, either by staining or by the cell specific expression of fluorescent proteins. I will study inflammation in this system, and characterise how it resolves. Once I have done this, I will test the ability of a range of candidate genes to influence the resolution of inflammation. These candidates include genes I have previously shown to have the potential to be important in the regulation of neutrophil apoptosis. These would include members of the death receptor family (TRAIL Receptors and Fas), the Bcl-2 family (regulators of apoptosis, including Bid and Bim), and the caspase family (executioner proteins that are involved in cell death, including caspases -1, -3, and -8). To study these genes in inflammation I will use a variety of techniques. These include neutrophil specific transgenic overexpression of genes on interest and inhibitors of gene function, Morpholino-modified antisense oligonucleotide knockdown of gene function, and screening of mutants for deletion of genes of interest (TILLING).
In addition, I will set up an unbiased screen to identify all genes (known or unknown) that are important in regulating apoptosis. This will be performed by ethylnitrosourea mutagenesis of founder males, and screening of third generation offspring for abnormal resolution of inflammation in the model studied in the earlier part of the study. These studies aim to identify the genes important in the resolution of inflammation, and so understand how this is disregulated in chronic inflammatory disease.

Publications

10 25 50
 
Description BBSRC Project Grant
Amount £373,297 (GBP)
Funding ID BB/L000830/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2013 
End 09/2016
 
Description MRC Senior Clinical Fellowship
Amount £1,538,980 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 08/2008 
End 11/2014
 
Description Wellcome Trust Project Grant
Amount £368,203 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2010 
End 01/2013
 
Description Wellcome Trust Project Grant (In vivo regulation of neutrophil function by hypoxic signalling)
Amount £189,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2007 
End 01/2010
 
Title Neutrophil specific transgenic zebrafish 
Description Using the neutrophil-specific mpx promoter (zebrafish myeloperoxidase equivalent), GFP is expressed in the neutrophils of living zebrafish. This enables study of neutrophil behaviour and function in vivo. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2006 
Provided To Others? Yes  
Impact Publications: 18802111, 19698716 others in progress Patent application Further funding (Senior Clinical Fellowship, Wellcome Trust Project grant) Demonstrated to public at Royal Society Summer Science Exhibition. 
 
Title mpx:Gal4 transgenic zebrafish line 
Description A neutrophil specific Gal4 transgenic zebrafish, allowing for genetic manipulation of neutrophil function in vivo. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2010 
Provided To Others? Yes  
Impact Only recently developped. No impacts yet. Will be able to replace some mammals in inflammation research with larval forms of zebrafish not protected under the Animals (Scientific Procedures) Act, and of a lower neurophysiological sensitivity. We would expect this to have significant impact on 3Rs. 
 
Description An in vivo drug screen for identifying new anti-inflammatory compounds 
Organisation FusionIP
Country United Kingdom 
Sector Private 
PI Contribution We have established a compound screen for novel antiinflamatory agents in our zebrafish model. This screen is currently being patented. We are in discussion with Pharmaceutical companies about performing these screens commercially, and are seeking grant funding, but as yet these have not been fully realised.
Collaborator Contribution Over the last 3-4 years we have had a number of useful discussions about commercialisation of this idea. FusionIP commercialise IP from our university. They have supported a patent application for this technology, and we are currently taking this forward within an academic setting, funded by the faculty of medicine.
Impact Patent application dropped. Publication: 19850882. Further punlication in revision for DMM.
Start Year 2006
 
Description Burkholderia in vivo project 
Organisation National Institute of Health and Medical Research (INSERM)
Department Nimes (INSERM)
Country France 
Sector Public 
PI Contribution We have provided reagents and expertise for the study of infectious agents in the zebrafish.
Collaborator Contribution Using reagents we developped and experiments designed in collaboration with us, this group have developed an in vivo model of Burkholderia infection, which is shedding new light on the mechanisms of pathogenicity of this bacteria.
Impact Publication. PMID: 20086083
Start Year 2008
 
Description Colitis in Zebrafish 
Organisation Erasmus University Medical Center
Country Netherlands 
Sector Academic/University 
PI Contribution Provided reagents and expertise for a collaborative project.
Collaborator Contribution Provided expertise in colitits.
Impact Publication. PMID: 19698716
Start Year 2007
 
Description Cytokine Signalling in Zebrafish 
Organisation University of Lisbon
Department Institute for Molecular Medicine
Country Portugal 
Sector Academic/University 
PI Contribution Exchange of ideas, protocols, reagents, collaborative projects
Collaborator Contribution Exchange of ideas, protocols, expertise.Collaborative effort on cytokine project.
Impact Publications: PMID: 18802111, 23509368 Transgenic line:NFkB:GFP.Luciferase
Start Year 2007
 
Description Cytokine Signalling in Zebrafish 
Organisation University of Murcia, Spain
Country Spain 
Sector Academic/University 
PI Contribution Exchange of ideas, protocols, reagents, collaborative projects
Collaborator Contribution Exchange of ideas, protocols, expertise.Collaborative effort on cytokine project.
Impact Publications: PMID: 18802111, 23509368 Transgenic line:NFkB:GFP.Luciferase
Start Year 2007
 
Description Cytokines in Zebrafish 
Organisation University of Murcia, Spain
Country Spain 
Sector Academic/University 
PI Contribution Contributed reagents and expertise to collaborative project.
Collaborator Contribution Expertise in zebrafish cytokines, and TLRs
Impact Publications: PMID: 18802111 Human Frontiers for Science collaborative project application (unsuccessful).
Start Year 2007
 
Description Forward genetic Screen 
Organisation Max Planck Society
Department Max Planck Institute for Developmental Biology
Country Germany 
Sector Academic/University 
PI Contribution Provided staff and expertise to perform the screen.
Collaborator Contribution Provided access and expertise in forward genetic (ENU) screening, to allow us to perform the first forward genetic screen looking at neutrophil functions in vivo.
Impact We have a panel of interesting mutants, and this panel was used as preliminary data in my MRC Senior Clinical Fellowship award. The mutants elude identification at present, but manuscripts will be forthcoming.
 
Description Hypoxia in zebrafish inflammation 
Organisation Leiden University
Department Institute of Biology Leiden
Country Netherlands 
Sector Academic/University 
PI Contribution Provided reagents, tools and expertise and supervision to post-doc working on thsi project.
Collaborator Contribution Developped new techniques of use in other areas of research, provided senior post-doctoral support to the lab.Contributed expertise, developped tools together for use in studying inflammation in vivo. The post-doc on this project has now secured an independent fellowship to move to Leiden where he continues to work on collaborative projects.
Impact Funding: Wellcome Trust project grant: £188853 PMID: 21748682 PMID: 22665266 PMID: 21555741 PMID: 24196071 Further manuscripts in submission.
Start Year 2007
 
Description Hypoxia in zebrafish inflammation 
Organisation University of Sheffield
Department Department of Infection, Immunity and Cardiovascular Disease
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided reagents, tools and expertise and supervision to post-doc working on thsi project.
Collaborator Contribution Developped new techniques of use in other areas of research, provided senior post-doctoral support to the lab.Contributed expertise, developped tools together for use in studying inflammation in vivo. The post-doc on this project has now secured an independent fellowship to move to Leiden where he continues to work on collaborative projects.
Impact Funding: Wellcome Trust project grant: £188853 PMID: 21748682 PMID: 22665266 PMID: 21555741 PMID: 24196071 Further manuscripts in submission.
Start Year 2007
 
Description Hypoxia in zebrafish inflammation 
Organisation University of Sheffield
Department MRC Centre for Developmental and Biomedical Genetics
Country United Kingdom 
Sector Public 
PI Contribution Provided reagents, tools and expertise and supervision to post-doc working on thsi project.
Collaborator Contribution Developped new techniques of use in other areas of research, provided senior post-doctoral support to the lab.Contributed expertise, developped tools together for use in studying inflammation in vivo. The post-doc on this project has now secured an independent fellowship to move to Leiden where he continues to work on collaborative projects.
Impact Funding: Wellcome Trust project grant: £188853 PMID: 21748682 PMID: 22665266 PMID: 21555741 PMID: 24196071 Further manuscripts in submission.
Start Year 2007
 
Description Il-1 processing and release 
Organisation University of Sheffield
Department Department of Cardiovascular Science
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided ZF knowledge and expertise to a mammalian cell biology project to allow in vivo study.
Collaborator Contribution Colllaborative effort to study Il-1b processing, release and targetting in vivo.
Impact A number of transgenic reagents have been generated, or are in the process of being generated. Manuscripts and grant applications are planned in the new year. Transgenic reagents produced: UAS:Il-1.GFP line UAS:Il-1.mCherry Mpeg1:il-1.GFP line BBSRC project grant BHF project grant Manuscript in Disease Models and Mechanisms.
Start Year 2008
 
Description In vivo modelling of Staphylococcus aureus infection. 
Organisation University of Sheffield
Department Department of Molecular Biology and Biotechnology
Country United Kingdom 
Sector Academic/University 
PI Contribution We have provided technical and theoretical input into the generation of a novel in vivo model of S. aureus infection in the zebrafish. I was lead supevisor for the PhD student who performed the experiments.
Collaborator Contribution Expertise on S. aureus infection, co-supervisor of PhD student, co-applicant in successful Wellcome Trust project grant. Sum quoted above is total amount of grant application. I am co-applicant, but will receive benefit from much of this.
Impact Publications: 18715285,24002065,22694745 Grant award: Project Grant funding from Wellcome Trust: £368203 Cross cutting studentship network awarded to this collaborative network, with funding for 3 students over 3 years. Eu FP7 Initial Training Network 524,000 Euros to Sheffield Multidiscipliinary: Microbiology, ecological modelling, zebrafish immunology MRC Project grant submitted September 2012 unsuccessful Manuscript in PLoS pathogens Submission to AMR2 call.
Start Year 2006
 
Description Macrophage project. 
Organisation University of Sheffield
Department MRC Centre for Developmental and Biomedical Genetics
Country United Kingdom 
Sector Public 
PI Contribution Provided expertise in generation of transgenic zebrafish, and in the study of immune cell biology in vivo.
Collaborator Contribution Developed tools collaboratively for the study of macrophages in vivo.
Impact PMID: 21225092 Recruited Felix Ellett from Australia on AHMRC CJ Martin fellowship to work on this project.
Start Year 2008
 
Description Neutrophil phagocytosis in vivo 
Organisation Pasteur Institute, Paris
Department Macrophages and Development of Immunity Pasteur Institute
Country France 
Sector Academic/University 
PI Contribution Provided reagents and expertise to a collaborative project.
Collaborator Contribution Provided expertise and performed experiments collaboratively
Impact PMID: 21868367
Start Year 2009
 
Description Neutrophil tracking in Durif mutants 
Organisation Monash University
Department Australian Regenerative Medicine Institute (ARMI)
Country Australia 
Sector Academic/University 
PI Contribution Developed tools and performed analysis of tracking data.
Collaborator Contribution Developed new tools for the study of neutrophil function in vivo, using analysis of data supplied by collaborators.Developped software tools collaboratively for the study of neutrophil function in vivo. Collaborator from WEHI has now moved to Monash. Contributed biological datasets for analysis, and experimental data for manuscript. Collaborator from Oncology Department, University of Sheffield has now moved to Sussex. Contribution of image processing expertise and algorithms.
Impact PMID: 22940471 Grant application submitted in Australia by collaborators, named collaborator on that grant Multidisciplinary: Biology, and image analysis (computational/programming).
Start Year 2008
 
Description Neutrophil tracking in Durif mutants 
Organisation The Walter and Eliza Hall Institute of Medical Research (WEHI)
Country Australia 
Sector Academic/University 
PI Contribution Developed tools and performed analysis of tracking data.
Collaborator Contribution Developed new tools for the study of neutrophil function in vivo, using analysis of data supplied by collaborators.Developped software tools collaboratively for the study of neutrophil function in vivo. Collaborator from WEHI has now moved to Monash. Contributed biological datasets for analysis, and experimental data for manuscript. Collaborator from Oncology Department, University of Sheffield has now moved to Sussex. Contribution of image processing expertise and algorithms.
Impact PMID: 22940471 Grant application submitted in Australia by collaborators, named collaborator on that grant Multidisciplinary: Biology, and image analysis (computational/programming).
Start Year 2008
 
Description Neutrophil tracking in Durif mutants 
Organisation University of Sheffield
Department Department of Oncology and Metabolism
Country United Kingdom 
Sector Academic/University 
PI Contribution Developed tools and performed analysis of tracking data.
Collaborator Contribution Developed new tools for the study of neutrophil function in vivo, using analysis of data supplied by collaborators.Developped software tools collaboratively for the study of neutrophil function in vivo. Collaborator from WEHI has now moved to Monash. Contributed biological datasets for analysis, and experimental data for manuscript. Collaborator from Oncology Department, University of Sheffield has now moved to Sussex. Contribution of image processing expertise and algorithms.
Impact PMID: 22940471 Grant application submitted in Australia by collaborators, named collaborator on that grant Multidisciplinary: Biology, and image analysis (computational/programming).
Start Year 2008
 
Description Neutrophil tracking in Durif mutants 
Organisation University of Sussex
Department School of Engineering and Informatics Sussex
Country United Kingdom 
Sector Academic/University 
PI Contribution Developed tools and performed analysis of tracking data.
Collaborator Contribution Developed new tools for the study of neutrophil function in vivo, using analysis of data supplied by collaborators.Developped software tools collaboratively for the study of neutrophil function in vivo. Collaborator from WEHI has now moved to Monash. Contributed biological datasets for analysis, and experimental data for manuscript. Collaborator from Oncology Department, University of Sheffield has now moved to Sussex. Contribution of image processing expertise and algorithms.
Impact PMID: 22940471 Grant application submitted in Australia by collaborators, named collaborator on that grant Multidisciplinary: Biology, and image analysis (computational/programming).
Start Year 2008
 
Description Neutrophil tracking in vivo 
Organisation City, University of London
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided datasets for analysis, and intellectual input and guidance into experimental design and interpretation.
Collaborator Contribution We have 2 students which we co-supervise, working on this project.Provided image analysis expertise to this project.Provides image processing expertise and algorithms to this project.
Impact 2 Studentships. PMID: 22951343 PMID: 22899935 PMID: 22940471 PMID: 22563379 PPMID: 24023630 Grant applications submitted: Human Frontiers for Science project - unsuccessful; BBSRC project grant - awarded 2013
Start Year 2008
 
Description Neutrophil tracking in vivo 
Organisation University of Sheffield
Department Automatic Control and Systems Engineering
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided datasets for analysis, and intellectual input and guidance into experimental design and interpretation.
Collaborator Contribution We have 2 students which we co-supervise, working on this project.Provided image analysis expertise to this project.Provides image processing expertise and algorithms to this project.
Impact 2 Studentships. PMID: 22951343 PMID: 22899935 PMID: 22940471 PMID: 22563379 PPMID: 24023630 Grant applications submitted: Human Frontiers for Science project - unsuccessful; BBSRC project grant - awarded 2013
Start Year 2008
 
Description Neutrophil tracking in vivo 
Organisation University of Sheffield
Department Department of Oncology and Metabolism
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided datasets for analysis, and intellectual input and guidance into experimental design and interpretation.
Collaborator Contribution We have 2 students which we co-supervise, working on this project.Provided image analysis expertise to this project.Provides image processing expertise and algorithms to this project.
Impact 2 Studentships. PMID: 22951343 PMID: 22899935 PMID: 22940471 PMID: 22563379 PPMID: 24023630 Grant applications submitted: Human Frontiers for Science project - unsuccessful; BBSRC project grant - awarded 2013
Start Year 2008
 
Description Neutrophil tracking in vivo 
Organisation University of Sussex
Department School of Engineering and Informatics Sussex
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided datasets for analysis, and intellectual input and guidance into experimental design and interpretation.
Collaborator Contribution We have 2 students which we co-supervise, working on this project.Provided image analysis expertise to this project.Provides image processing expertise and algorithms to this project.
Impact 2 Studentships. PMID: 22951343 PMID: 22899935 PMID: 22940471 PMID: 22563379 PPMID: 24023630 Grant applications submitted: Human Frontiers for Science project - unsuccessful; BBSRC project grant - awarded 2013
Start Year 2008
 
Description Pharmacological manipulation of regeneration pathways 
Organisation Oregon State University
Department Department of Environmental and Molecular Toxicology
Country United States 
Sector Academic/University 
PI Contribution Provided reagents and expertise, and performed experiments.
Collaborator Contribution Worked collaboratively on a project to identify pharmacological regulators of regeneration.
Impact Publication: PMID: 17848559
Start Year 2007
 
Description Polymersomes for neutrophil manipulation 
Organisation University College London
Department Department of Chemistry
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise in neutrophil biology, co-supervised student.
Collaborator Contribution Expertise on polymer some biology, co-supervised student.
Impact MRC CDBG Studentship PMID: 23033321 Other manuscripts in preparation.
Start Year 2009
 
Description Polymersomes for neutrophil manipulation 
Organisation University of Sheffield
Department Department of Biomedical Science
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise in neutrophil biology, co-supervised student.
Collaborator Contribution Expertise on polymer some biology, co-supervised student.
Impact MRC CDBG Studentship PMID: 23033321 Other manuscripts in preparation.
Start Year 2009
 
Description Singapore inflammation project 
Organisation Agency for Science, Technology and Research (A*STAR)
Department Institute of Molecular and Cell Biology,
Country Singapore 
Sector Academic/University 
PI Contribution Expertise, shared experimental design and manscuript preparation.
Collaborator Contribution generation of shared reagents, sharing expertise
Impact New technology generated, tools shared. PMID: 21555741 Drug screen manuscript published in Disease Models and Mechanisms PMID: 24291762
Start Year 2008
 
Description Zebrafish inflammation assay benchmarking 
Organisation GlaxoSmithKline (GSK)
Department Respiratory Centers for Excellence for Drug Discovery CEDD GSK
Country United Kingdom 
Sector Private 
PI Contribution We are testing a range of pharmacologically active anti-inflammatory compounds in a range of zebrafish assays of the inflammatory response, to compare the assay against murine models.
Collaborator Contribution Provision of reagents.
Impact Collaboration involves pharmacology and synthetic chemistry expertise in GSK. Data collection complete, manuscript in Science Translational Medicine, Manuscript in revision for Disease Models and Mechanisms. Award of MRC Fellowship Partnership Award building on this collaboration
Start Year 2009
 
Description miRNA analysis in human neutrophils 
Organisation University of Sheffield
Department Department of Cardiovascular Science
Country United Kingdom 
Sector Academic/University 
PI Contribution Performed experiments, and data analysis, experimental design, intellectual input into all stages of experiments and manuscript generation.
Collaborator Contribution Provided expertise on data analysis.Provided expertise on data analysis
Impact PMID: 21283524
Start Year 2008
 
Description miRNA analysis in human neutrophils 
Organisation University of Sheffield
Department Department of Oncology and Metabolism
Country United Kingdom 
Sector Academic/University 
PI Contribution Performed experiments, and data analysis, experimental design, intellectual input into all stages of experiments and manuscript generation.
Collaborator Contribution Provided expertise on data analysis.Provided expertise on data analysis
Impact PMID: 21283524
Start Year 2008
 
Title Screen for inflammatory response modifiers 
Description Patent application in progress to protect the use of neutrophil specific transgenic zebrafish for use in a screen to identify novel anti-inflammatory compounds. 
IP Reference WO2007135367 
Protection Patent application published
Year Protection Granted 2007
Licensed No
Impact Publication: 19850882 Commercial opportunities currently under development. This has now been allowed to lapse for commercial reasons.
 
Description COPD8 talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Health professionals
Results and Impact Spoke at COPD conference on emerging models for the study of COPD.

Raised profile of ZF for study of COPD/neutrophils.
Year(s) Of Engagement Activity 2012
 
Description ECCMID talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Invited talk to European Congress on infectious disease and clinical microbiology (ECCMID).

Raised profile of zebrafish infection work.
Year(s) Of Engagement Activity 2012
 
Description NC3Rs symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach National
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact NC3Rs, Society of Biology symposium.
Zebrafish to study inflammation: How this can reduce rodent use in inflammation research

Asked to join NC3Rs studentship Panel.
Made contact with a number of groups
Year(s) Of Engagement Activity 2013
URL http://www.nc3rs.org.uk/event.asp?latest=1&id=1930
 
Description Poster presentation to British Thoracic Society 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Health professionals
Results and Impact Poster presentation of work to respiratory physicians and scientists.

Increased profile of research to a clinical audience not familiar with zebrafish as a model.
Year(s) Of Engagement Activity 2007
 
Description Royal Society Summer Science Exhibition 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Contributed to design and staffing of a stand at the Royal Society Summer Science Exhibition, demonstrating how zebrafish are able to help us understand and treat human disease.

Much public interest in use of zebrafish as a model of human disease. Raising profile of MRC with general public.
Year(s) Of Engagement Activity 2009
 
Description Schools workshops 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Held workshops for school children aged 10-12 to help them understand the innate immune system and neutrophil function. This included some data from my MRC funded work, which illustrates this well.

Increased understanding of local schools and schoolchildren of innate immune system, and tools available for its study.
Year(s) Of Engagement Activity 2007,2008
 
Description Talk to British Thoracic Society 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach National
Primary Audience Health professionals
Results and Impact 2009: Presented results of compound screen performed in zebrafish to audience of respiratory professionals.
2010: presented results of in vivo neutrophil fate experiments. Talk given by BMedSci student.

Increased awareness of novel basic science (and particularly zebrafish) approaches to drug discovery for respiratory disease. Raised profile of lab group, and MRC in health professionals.
Year(s) Of Engagement Activity 2009,2010
 
Description ZDM6 meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Presented unpublished data on PGE2 and inflammation resolution.

Established dialogue with Anna Huttenlocher, key person in this field.
Year(s) Of Engagement Activity 2013
 
Description ZDM8 Boston MA. September 2015 Invited Speaker. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact ZDM8 Boston MA. September 2015 Invited Speaker.
Year(s) Of Engagement Activity 2015