Expansion of the Metabolomic and Cardiovascular Disease Facility at Cambridge University

Lead Research Organisation: University of Cambridge

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Summary

Cambridge University was awarded #665K to expand the Metabolic and Cardiovascular disease facility for the development of mouse models. This will add significantly to the existing strengths at Cambridge, and directly addressed the MRC strategic priority of developing models of human disease.|Metabolic and cardiovascular diseases are major public health challenges. Additionally, therapeutic options for these disorders are very limited and thus, they represent, to industry, a considerable unmet need. Cardio-metabolic diseases are quintessentially disorders of systems, not simply cells, and studies in whole organisms are therefore essential to both the elucidation of their pathophysiology and the examination of possible novel therapies. The pharmaceutical and biotechnology industries are increasingly looking to partner with sophisticated academic laboratories to undertake sophisticated target validation and proof of concept studies in appropriately designed and refined animal models. The UK academic sector, in general, is not particularly well placed to respond to this need. However, there are key centres of excellence in specific disease areas that are uniquely placed to fulfil those needs. Cambridge University may represent such a centre in cardio-metabolic disease and the award will helps to improve the Universitys ability to carry out detailed phenotypic analysis of murine models of human metabolic and cardiovascular disease. The development and utilization of sophisticated phenotyping technologies are crucial to capitalise on the power of murine genetic manipulation and facilitate meaningful translation of basic science into the clinical arena. Cambridge aim to incorporate state of the art technologies and develop sophisticated, accurate protocols in two major phenotyping areas: 1) the characterization of central and peripheral mechanisms controlling energy balance (e.g. food intake, energy expenditure and fat deposition); and 2) the evaluation of cardiovascular complications such as hypertension, dyslipidaemia and macrovascular disease that are strongly associated with metabolic diseases such as obesity and diabetes mellitus.

Publications

10 25 50

publication icon
Clemente FJ (2014) A Selective Sweep on a Deleterious Mutation in CPT1A in Arctic Populations. in American journal of human genetics

publication icon
Patel MN (2015) Hematopoietic IKBKE limits the chronicity of inflammasome priming and metaflammation. in Proceedings of the National Academy of Sciences of the United States of America

 
Title TVPLAB twitter 
Description Use of Social Media TVPLAB TWITTER https://twitter.com/tvplab?lang=en-gb 
Type Of Art Creative Writing 
Year Produced 2015 
Impact Creative tool to communicate our science to public 
URL https://twitter.com/tvplab?lang=en-gb
 
Description • International Scientific Advisory Board Danish Academy of Diabetes (2018-)
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
Impact • International Scientific Advisory Board Danish Academy of Diabetes (2018-) Advice on training and research priorities.
URL https://www.danishdiabetesacademy.dk/news/dda-appoints-strong-new-international-advisory-board
 
Description Cambridge-NUHS Seed Fund
Amount $35,000 (SGD)
Funding ID Study of FIT FAT 
Organisation National University Health System (NUHS) 
Sector Public
Country Singapore
Start  
 
Description MRC MDU Programme Grant
Amount £2,000,000 (GBP)
Funding ID MRC MDU Programme Grant 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 04/2013 
End 03/2018
 
Title 3D Culture of Stem cells 
Description 3D tissue culture system to study cross talk between stem cells 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact Improvement in quality of results by better mimicking in vivo intercellular interactions. 
 
Title Development of the Phenomics center 
Description Development of three core facilities, a) Murine phenotyping, b) Murine Biochemistry and c) Murine Histopathology 
Type Of Material Improvements to research infrastructure 
Year Produced 2006 
Provided To Others? Yes  
Impact Development of MRC CORD murine metabolic programme Development and expansion vreating the Cambridge Phenomics centre. 
 
Title IT support for data storage and backup 
Description This enables us to have a reliable data storage system to reproduce the mass of data produced in metabolic phenotyping. Furthermore, with a goad data storage system we are able to analyze data in various ways. 
Type Of Material Biological samples 
Provided To Others? No  
Impact Refinement and reduction 
 
Title Mass Spectrometry to analyse lipids 
Description Refined technology to measure lipid composition. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2015 
Provided To Others? Yes  
Impact 1. Yew Tan C, Virtue S, Murfitt S, Robert LD, Phua YH, Dale M, Griffin JL, Tinahones F, Scherer PE, Vidal-Puig A. Adipose tissue fatty acid chain length and mono-unsaturation increases with obesity and insulin resistance. Sci Rep. 2015 Dec 17;5:18366. doi: 10.1038/srep18366. 2. Tan CY, Virtue S, Bidault G, Dale M, Hagen R, Griffin JL, Vidal-Puig A. Brown Adipose Tissue Thermogenic Capacity Is Regulated by Elovl6. Cell Rep. 2015 Dec 15;13(10):2039-47. doi: 10.1016/j.celrep.2015.11.004. Epub 2015 Nov 25. 3. Senyilmaz D, Virtue S, Xu X, Tan CY, Griffin JL, Miller AK, Vidal-Puig A, Teleman AA. Regulation of mitochondrial morphology and function by stearoylation of TFR1. Nature. 2015 Jul 27. doi: 10.1038/nature14601. 
 
Title b) Design a unique in house system for energy balance measurement. 
Description This is a new cageingsystem for metabolicphenotyping for simultaneous assessment of food inatke, energy expnditure, activity, water consumption. The equipment incorporates novel features such as having food delivery system hook to the cover of the cage. Also this is system is adapted to the cages where are normally hold which prevents measurement artifacts derived from changes in the cages when performing metabolic measurements. The new developed system to assess energy balance is a refinement because we can measure more accurately in each experiment. The addition of a 24h camera system with Visual Sonics software enables us to have secure systems to observe without disturbing measurements. 
Type Of Material Physiological assessment or outcome measure 
Year Produced 2010 
Provided To Others? Yes  
Impact Reduction and refinement of experiments using murine models 
 
Title Bionash 
Description Data base of human liver biopsies 
Type Of Material Database/Collection of data 
Year Produced 2016 
Provided To Others? Yes  
Impact Contribution to International consortiums such fp7 Epos and FP7 IMI LItmus 
URL http://www.epos-nafld.eu/
 
Description LPGD2S KO MOUSe 
Organisation Osaka University
Country Japan 
Sector Academic/University 
PI Contribution Phenotyping of mouse models
Collaborator Contribution provided animal models for MRC funded project.
Impact Papers still under submission expected to be published during 2010
Start Year 2007
 
Description Plasticell: "In vivo activation of brown adipose tissue by plant-derived natural extracts", Innovation 
Organisation Plasticell Ltd
Country United Kingdom 
Sector Private 
PI Contribution Knowledge on mouse murine phenotyping
Collaborator Contribution Technology in conbinatorial optimisation of Stem cell differentiation.
Impact Multidisciplinary combining our unique expertise
Start Year 2017
 
Description 36 Congreso Sociedad Española de Farmacología. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact http://congresosef2015.com/programa-cientifico/esquema-del-programa/
12:30 - 13:30
Sesión Plenaria.

Moderadora: Dra. Mª Jesús Sanz Ferrando
Universitat de València.
Adipose tissue expandability, lipotoxicity and the metabolic syndrome.
Prof. Antonio Vidal Puig University of Cambridge. Reino Unido.
Year(s) Of Engagement Activity 2015
URL http://congresosef2015.com/programa-cientifico/esquema-del-programa/
 
Description 5th - 9th June 2015. ADA Scientific Sessions Insulin Action/Molecular Metabolism. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Lecture American Diabetes Association Conference 2015
Year(s) Of Engagement Activity 2015
URL http://professional2.diabetes.org/Presentations_Details.aspx?typ=10&sr=adv&cgr=238&congress=238
 
Description Barcelona, Spain. 24th-27th May 2017. EMBO workshop meeting on BAT. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Barcelona, Spain. 24th-27th May 2017. EMBO workshop meeting on BAT. Conference lecturer
Year(s) Of Engagement Activity 2017
URL http://meetings.embo.org/event/17-brown-fat
 
Description Dusseldorf, Germany. 10th October 2017. Universitäts Klinikum Lecture Series - CRC1116 "master switches after myocardial infarction" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Dusseldorf, Germany. 10th October 2017. Universitäts Klinikum Lecture Series - CRC1116 "master switches after myocardial infarction"
Year(s) Of Engagement Activity 2017
URL http://www.irtg1902.hhu.de/schedule/schedule-single-view.html?tx_cal_controller%5Bgetdate%5D=2017101...
 
Description EAS Advanced Course in Cardiometabolic Phenotyping in Rodents. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact EAS Advanced in Cardiometabolic Phenotyping in Rodents, Cambridge, UK

Scientists from the University of Cambridge Biomedical Campus recently hosted the 2nd EAS Advanced Course in Cardiometabolic Phenotyping in Rodents. This 3-day course combines the state of the art facilities and expertise within the Disease Model Core at the WellcomeTrust-MRC Institute of Metabolic Science and the BHF Cardiovascular Centre. By integrating the expertise of these two specialized phenotyping centres, it provides a unique opportunity to learn about many of the skills required for in depth cardiometabolic phenotyping in small animals.

The 32 participants, selected from centres across Europe, were treated to a programme of lectures, phenotyping demonstrations and data analysis sessions. A further 1-day workshop at the Sanger/EBI focused on mouse genetics, genetic engineering and informatics resources, highlighting the great opportunities provided by recent advances in genetic engineering purpose-made mouse models. Participants particularly appreciated the mixture of hands-on experience, combined with opportunities to ask detailed questions on how the techniques demonstrated should be used to suit their own research priorities.

The course was sponsored by the European Atherosclerosis Society, British Heart Foundation and TSE systems, with organisation led by Prof Toni Vidal-Puig, Director of the MRC Metabolic Diseases Unit Disease Model Core.
Year(s) Of Engagement Activity 2015
URL http://tvp.mrl.ims.cam.ac.uk/events/
 
Description European Neuroendocrinology Society meeting in Vienna. . 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Dr Sam Virtue recently presented at the prestigious European Neuroendocrinology Society meeting in Vienna. Sam's talk was focussed on metabolomics in endocrinology and was based on the past 9 years of experience in the TVP lab in the field of lipodomics. The purpose of the talk was to discuss how biologists without systems biology experience can work with groups specialising in the fields of metabolomics to design appropriate experiments and extract the maximum value from their data sets.

Dr Virtue has been invivted to give similar type of talk in other venues,
Year(s) Of Engagement Activity 2012
 
Description Keystone Symposia on Diabetes/Obesity and Adipose Tissue Biology. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Keystone Conference Lecture. Colorado, USA. 22nd - 27th January 2017.
Year(s) Of Engagement Activity 2017
URL http://healthdocbox.com/amp/69735120-Diabetes/Diabetes-january-22-26-2017-keystone-conference-center...
 
Description Lisbon, Portugal. 11th-15th September 2017. 53rd EASD Annual Meeting. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Lisbon, Portugal. 11th-15th September 2017. 53rd EASD Annual Meeting. >150 attendants to symposium on Obesity associated metabolic complications.
Year(s) Of Engagement Activity 2017
URL https://d-net.idf.org/en/events/369-53rd-easd-annual-meeting-in-lisbon-portugal.html
 
Description Majorca, Spain. 16th October 2017. Seminar at Illes Balears Institute for Health Research. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Majorca, Spain. 16th October 2017. Seminar at Illes Balears Institute for Health Research. Research seminar. >80 attendees.
Year(s) Of Engagement Activity 2017
URL https://www.arabalears.cat/balears/Antonio-Vidal-Puig-nostres-estalviadors-lluitar_0_1892210855.html
 
Description Naples, Italy. 28th May 2018. Seminar at The Institute of Genetics and Biophysics "Adriano Buzzati-Traverso" of the National Research Council (CNR). 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Naples, Italy. 28th May 2018. Seminar at The Institute of Genetics and Biophysics "Adriano Buzzati-Traverso" of the National Research Council (CNR). Lecture on the Adipose tissue expandability, lipotoxicity and metabolic syndrome.
Year(s) Of Engagement Activity 2018
 
Description 4. Bradford, UK. 1st December 2018. SRUK Seminar (Society of Spanish Researchers in the UK). 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact 4. Bradford, UK. 1st December 2018. SRUK Seminar (Society of Spanish Researchers in the UK).
Year(s) Of Engagement Activity 2018
URL https://www.eventbrite.co.uk/e/v-christmas-seminar-science-of-healthy-diet-sports-wellbeing-tickets-...
 
Description 5. London, UK. 30th October 2018. California Walnut Commission Scientific & Health Research Meeting. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact 5. London, UK. 30th October 2018. California Walnut Commission Scientific & Health Research Meeting.
Year(s) Of Engagement Activity 2018
URL https://www.bighospitality.co.uk/News/Promotional-Features/California-Walnut-Culinary-Institute-of-A...
 
Description Bilbao, Spain. 8th June 2018. Seminar at Biogune. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 7. Bilbao, Spain. 8th June 2018. Seminar at Biogune.
Year(s) Of Engagement Activity 2018
URL https://twitter.com/CICbioGUNE
 
Description Cambridge Metabolic Network/Danish Diabetes Association Course for PhD Students. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Course sponsored by NOVO foundation from Denmark, 35 PhD students from Cambridge and Denmark.
Year(s) Of Engagement Activity 2017
URL https://www.danishdiabetesacademy.dk/events/lipid-metabolism-new-methods-and-translational-aspects
 
Description Form the future Careers Carousel at Sawston Village College 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Form the future Careers Carousel at Sawston Village College
Year(s) Of Engagement Activity 2017
 
Description LASA (Laboratory Animal Science Association) IAT (Institute of Animal Technology) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact More than 100 LASA members attended and the content was specifically related to either maintenance of specific animal models, thermoregulation (technical challenges in achieving correct housing temperatures) as well as a in house design for small animal metabolic phenotyping.

We have been approached by other academic institutions and companies to provide advice on metabolic phenotyping and to request technical support to develop their own programmes. Also we are increaisngly receiving request to review grants based on technology for murine phenotyping.
Year(s) Of Engagement Activity 2009,2010
 
Description MIddle EaSt obesity 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Professors Toni Vidal-Puig MD PhD FRCP FMedSci EMBA and Meis Moukayed have honoured us by writing the fascinating article, People with obesity: Their heroic battle to defeat their genes in a hostile hypercaloric sedentary environment. The professors explain why more than 50 human genes, the environment and lifestyle culture all contribute to why it is not so easy as the doctor telling the patient to lose weight. In addition to looking at the 'why' of obesity,
Year(s) Of Engagement Activity 2018
URL https://www.middleeastmedicalportal.com/product/november-december-2018/
 
Description Malaga, Spain. 20th-23rd March 2017. Danish Diabetes Academy Postdoc Winter School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact On March 19-24, 2017, a total of 99 Post Doc fellows, third-year PhD students and prominent national and international speakers attended Danish Diabetes Academy's 3rd Winter School in Malaga with focus on different aspects of prevention and treatment of diabetes as well as career development. Malaga, Spain. 20th-23rd March 2017. Danish Diabetes Academy Postdoc Winter School. .
Year(s) Of Engagement Activity 2017
URL https://www.danishdiabetesacademy.dk/node/1087
 
Description Paris, France. 11th-15th April 2018. EASL International Liver Congress. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation at the Paris, France. 11th-15th April 2018. EASL International Liver Congress focused on the pathogenesis of fatty liver and the effect of Lipotoxicty
Year(s) Of Engagement Activity 2018
URL https://easl.eu/discover/events/detail/2018/the-international-liver-congress-2018
 
Description Presentation at AALAS (American Association of Laboratory Animal Science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Health professionals
Results and Impact Attending more than 150 membersof AALAS, we presented our expertise in mouse husbandry and metabolic phenotyping technology development.

Increased demand for technical advice related to murine husbandry and technological developments for murine phenotyping.
Year(s) Of Engagement Activity 2009,2010
 
Description Presentation at the Institute of Safety in Technology and Research 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Participants in your research and patient groups
Results and Impact Presentations have been given regarding Health and Safety issues at ISTR (Institute of Safety in Technology and Research) on safe transport of biological materials.

Increased awareness on issues related to safe transport are expected to improve safety.
Year(s) Of Engagement Activity 2009,2010
 
Description Royal Swedish Academy of Sciences, Journal of Internal Medicine - Key Symposium "Metabolic complications of obesity" 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Organisation of symposia with 150 attendants, 28 speakers from EU, USA, JAPAN, CHINA. Top symposium supported by the Swedish Academy of Sciences and the Journal of Internal Medicine.
Year(s) Of Engagement Activity 2017
URL http://www.mrl.ims.cam.ac.uk/event/metabolic-complications-obesity/
 
Description Toulouse, France. 28th April 2017. Seminar at INSERM. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Toulouse, France. 28th April 2017. Seminar at INSERM. Dissemination of research outcomes to research commnity
Year(s) Of Engagement Activity 2017
URL https://www.stromalab.fr/actualites/pr-toni-vidal-puig-university-of-cambridge-uk/