External Collaboration, Innovation and Entrepreneurism: Translational Medicine in Exeter 2 (EXCITEME2)

Lead Research Organisation: University of Exeter

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Summary

The MRC Proximity to Discovery scheme awards universities funds to help develop new collaborations, and ways of exchanging knowledge and skills. The awards can be used to support activities that promote the value of academic-industry partnership, and enhance academic and industry researchers’ understanding of each other’s needs and capabilities. This may be through people exchanges, creation of technology demonstrators, showcase events, commercialisation workshops and ‘entrepreneurs in residence’ schemes. Such exchanges of knowledge and skills will boost the most fruitful collaborations between UK universities and life science companies.

Publications

10 25 50
 
Description BB/V008021/1 - "Understanding molecular accumulation in single cells via microfluidics and omics
Amount £540,000 (GBP)
Organisation United Kingdom Research and Innovation 
Sector Public
Country United Kingdom
Start 06/2020 
End 06/2021
 
Description Characterising the homology of pharmacological targets in aquatic life
Amount £102,026 (GBP)
Funding ID BB/V509346/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2020 
End 09/2024
 
Description EU/IMI (PREMIER- €1M, 2020-2025 part of €8M project on pharmaceuticals in the environment with pharmaceutical companies including AstraZeneca (Tyler)
Amount € 8,000,000 (EUR)
Organisation European Union 
Sector Public
Country European Union (EU)
Start 01/2020 
End 01/2025
 
Description Lattice Selective Plane Illumination Microscopy (L-SPIM) for the analysis of subcellular dynamics in living specimens.
Amount £653,837 (GBP)
Funding ID BB/T017899/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2020 
End 06/2021
 
Description Mechanisms and evolutionary consequences of host immunosuppression by anti-CRISPR phages
Amount £512,438 (GBP)
Funding ID BB/S017674/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2021 
End 12/2022
 
Description UKRI FLF £1.5M Oct2020-Sep2024
Amount £1,500,000 (GBP)
Organisation United Kingdom Research and Innovation 
Sector Public
Country United Kingdom
Start 10/2020 
End 09/2024
 
Description Understanding phenotypic plasticity and relevance of behaviour responses to chronic toxicity in aquatic life
Amount £102,026 (GBP)
Funding ID BB/V509358/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2020 
End 09/2024
 
Title Genome-wide genotyping of 10,142 PROTECT study samples has been completed in kind. 
Description Genome-wide genotyping of 10,142 PROTECT study samples has been completed in kind. We have collaborations agreements in place to share this data with sixother research groups at UK academic institutions and one in Norway. We are also sharing the data with the leading international Psychiatric Genomics Consortium who will be included the data in their next GWAS of major depression 
Type Of Material Data handling & control 
Year Produced 2020 
Provided To Others? No  
Impact Genome-wide genotyping of 10,142 PROTECT study samples has been completed in kind. We have collaborations agreements in place to share this data with sixother research groups at UK academic institutions and one in Norway. We are also sharing the data with the leading international Psychiatric Genomics Consortium who will be included the data in their next GWAS of major depression 
 
Title Genome-wide genotyping of PROTECT study samples 
Description Genome-wide genotyping of 10,142 PROTECT study samples has been completed in kind which will form part of the data made available by PROTECT to third party researchers 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? No  
Impact TBC 
 
Description An adaptive sampling tool for antibody candidate drug lead optimisation and developability assessment 
Organisation Applied Photophysics Ltd
Country United Kingdom 
Sector Private 
PI Contribution Graduate Research Assistant (GRA) - one FTE for 12 months at Graduate Research Assistant level (Grade E27) to perform hydrogen/deuterium-exchange mass spectrometry (HDX-MS) experiments. Consumables - The operation of the liquid chromatography mass spectrometry (LC-MS) system to do HDX-MS on immunoglobulin samples requires high purity He/Ar gases, LC-MS grade solvents and acids, isotopically labelled chemicals and molecular standards, dedicated digestion and trapping columns per project, high concentrations of an expensive reducing agent (tris-carboxyethylphosphine - TCEP) and denaturants and low-bind borosilicate glass vials for benchmarking experiments. Travel - This partnership will require two-way communication of key challenges (in terms of fundamental science and drug development), cross-training between partner laboratories and collection of critical data on instrumentation uniquely available in the partner laboratory (MedImmune, Cambridge). Regular project meetings will be held monthly by Skype to minimise costs. Technology transfer of the know-how in cutting-edge instrumentation and methods, developed at Univ. Exeter, will also be supported during these visits by the GRA to MedImmune. It is anticipated that supporting biophysical experiments will be undertaken at this time, using instrumentation available at MedImmune, but absent at Univ. Exeter (i.e. SEC-MAL(L)S; HDX-MS with Orbitrap Fusion; high-throughput DSC). Senior Engineer - We require the support of 5% FTE to implement software integration between the adaptive sampling tool and the instrumentation. This will allow for near- or full-automation. Partner (APL) has agreed to provide this essential support. PI 5% FTE - JJP will supervise the research project. GRA can independently acquire data. JJP will spend time in project strategy, experiment design and data analysis. Estates, infrastructure and indirect costs to be provided by College (CLES).
Collaborator Contribution Material supply of biopharmaceutically-relevant compounds (in-kind). High purity immunoglobulin-derived and antigen (e.g. PD-L1) molecules. Materials will be supplied at the start of the project, where possible. Cash value of therapeutic monoclonal antibodies is estimated at >£2.5k/mg; peptide hormones is >£1k/mg; peptide-Fc fusion proteins is >5k/mg; protein antigen is >£2.5k/mg. Use of instrumentation - Orbitrap Fusion, differential scanning calorimeter valued at £12,500 (5 x £2500 per week). Senior engineer - 5% FTE (in-kind) to implement software integration. £6,700 + overheads. Match funding £67,715 (£28,215 cash; £39,500 in-kind)
Impact Still active
Start Year 2019
 
Description An adaptive sampling tool for antibody candidate drug lead optimisation and developability assessment 
Organisation AstraZeneca
Department MedImmune
Country United Kingdom 
Sector Private 
PI Contribution Graduate Research Assistant (GRA) - one FTE for 12 months at Graduate Research Assistant level (Grade E27) to perform hydrogen/deuterium-exchange mass spectrometry (HDX-MS) experiments. Consumables - The operation of the liquid chromatography mass spectrometry (LC-MS) system to do HDX-MS on immunoglobulin samples requires high purity He/Ar gases, LC-MS grade solvents and acids, isotopically labelled chemicals and molecular standards, dedicated digestion and trapping columns per project, high concentrations of an expensive reducing agent (tris-carboxyethylphosphine - TCEP) and denaturants and low-bind borosilicate glass vials for benchmarking experiments. Travel - This partnership will require two-way communication of key challenges (in terms of fundamental science and drug development), cross-training between partner laboratories and collection of critical data on instrumentation uniquely available in the partner laboratory (MedImmune, Cambridge). Regular project meetings will be held monthly by Skype to minimise costs. Technology transfer of the know-how in cutting-edge instrumentation and methods, developed at Univ. Exeter, will also be supported during these visits by the GRA to MedImmune. It is anticipated that supporting biophysical experiments will be undertaken at this time, using instrumentation available at MedImmune, but absent at Univ. Exeter (i.e. SEC-MAL(L)S; HDX-MS with Orbitrap Fusion; high-throughput DSC). Senior Engineer - We require the support of 5% FTE to implement software integration between the adaptive sampling tool and the instrumentation. This will allow for near- or full-automation. Partner (APL) has agreed to provide this essential support. PI 5% FTE - JJP will supervise the research project. GRA can independently acquire data. JJP will spend time in project strategy, experiment design and data analysis. Estates, infrastructure and indirect costs to be provided by College (CLES).
Collaborator Contribution Material supply of biopharmaceutically-relevant compounds (in-kind). High purity immunoglobulin-derived and antigen (e.g. PD-L1) molecules. Materials will be supplied at the start of the project, where possible. Cash value of therapeutic monoclonal antibodies is estimated at >£2.5k/mg; peptide hormones is >£1k/mg; peptide-Fc fusion proteins is >5k/mg; protein antigen is >£2.5k/mg. Use of instrumentation - Orbitrap Fusion, differential scanning calorimeter valued at £12,500 (5 x £2500 per week). Senior engineer - 5% FTE (in-kind) to implement software integration. £6,700 + overheads. Match funding £67,715 (£28,215 cash; £39,500 in-kind)
Impact Still active
Start Year 2019
 
Description Developing a prototype microfluidic platform for gene expression profiling of single mammalian cells and bacteria 
Organisation Blacktrace Holdings Ltd
Country United Kingdom 
Sector Private 
PI Contribution P2D funding was requested for the full-time salary of a postdoctoral research associate for 6.5 months. After a three-month period of training with Senior UoE academic staff. The College will fund the salary of PI and CoI at 20% FTE for the first six months of the project. The College will fund the full-time salary of a PDRA for 4.5 months and travel to Penryn and Blacktrace via the LSI/Penryn Mash-up Initiative.
Collaborator Contribution Microfluidic devices and accessories: Blacktrace, will provide a number of microfluidic devices which are necessary for the success of this project. These include devices for isolating single cells, specialised microfluidic tubing and fluidic connectors for the functioning of these devices Test cells and consulting input for data analysis: Hummingbird will provide frozen test tumour cells and up to 20 days of time to assist with the analysis, with the overall aim of dissecting intratumoral heterogeneity in gene expression and identifying potential immunotherapy targets.
Impact Still active
Start Year 2018
 
Description Developing a prototype microfluidic platform for gene expression profiling of single mammalian cells and bacteria 
Organisation Hummingbird Bioscience Pte. Ltd
Country Singapore 
Sector Private 
PI Contribution P2D funding was requested for the full-time salary of a postdoctoral research associate for 6.5 months. After a three-month period of training with Senior UoE academic staff. The College will fund the salary of PI and CoI at 20% FTE for the first six months of the project. The College will fund the full-time salary of a PDRA for 4.5 months and travel to Penryn and Blacktrace via the LSI/Penryn Mash-up Initiative.
Collaborator Contribution Microfluidic devices and accessories: Blacktrace, will provide a number of microfluidic devices which are necessary for the success of this project. These include devices for isolating single cells, specialised microfluidic tubing and fluidic connectors for the functioning of these devices Test cells and consulting input for data analysis: Hummingbird will provide frozen test tumour cells and up to 20 days of time to assist with the analysis, with the overall aim of dissecting intratumoral heterogeneity in gene expression and identifying potential immunotherapy targets.
Impact Still active
Start Year 2018
 
Description Functional live brain imaging in zebrafish for mechanism-specific detection of drug-induced seizures 
Organisation AstraZeneca
Department Drug Safety and Metabolism Externalisation
Country United Kingdom 
Sector Private 
PI Contribution Most of the practical work, image analysis and data processing will be undertaken by a Senior Research Fellow (150 days) supported by a research technician (50 days), Data processing requires advanced systems learning and statistics developed by Marc Goodfellow (senior lecturer in mathematics) who will also provide advanced data processing support as needed. Time has been allocated for an element of protect monitoring to ensure outputs are delivered and documented, to facilitate regular project updates and the site exchange visits (10 days). £2000 has been allocated for travel/subsistence to facilitate project meetings with AZ.
Collaborator Contribution AstraZeneca are providing a considerable financial contribution to the project. This funding has been generated through the AZ Science to go innovation platform which aims to fund short-term research projects which have a business benefit as a likely outcome. This funding is not standalone; it is allocated on the understanding that supporting funding will become available from the university. The lead CNS safety experts at AZ DSM will also each provide in-kind support including: compound selection for each of the five target MOA, data interpretation and mammalian toxicology expertise, and exchange visits between this team and UoE researchers to disseminate results and to explore further research opportunities. The value of this time is estimated as at least £7,500.
Impact Still active
Start Year 2018
 
Description Identifying characteristics and behaviours associated with alert fatigue in a clinical decision support system to develop strategies to optimise the user interaction 
Organisation First Databank Europe Ltd
Country United Kingdom 
Sector Private 
PI Contribution The P2D allocation includes funds to cover 60% of a PDRA for 11 months (the duration of the project) as well as travel and subsistence money for the industrial secondment. The EPSRC Centre for Predictive Modelling have indicated they will fund the remaining 40% of the PDRA salary for the duration of the project. The college will provide in-kind contributions toward PI and CoI over the length of the 11 month project. The College will also cover indirect costs, estates and infrastructure for the duration of the project.
Collaborator Contribution Over the project period, the project partner will contribute as follows: For the data analysis (month 1 - 9) • 5 days of Clinical and Product specialist time to on-board over first month (5d) • A further 2 days per month of clinical and product input for 7 months (14d) • A further 5 days of clinical and product input for project closure (5d) • 0.5d/month of senior management input (4d) For Study Development (month 10-11) • 5 days Clinical team support over 2 months For hardware and IT support for the full 11 month (month 1-11) • Access to appropriate hardware and IT support In- Kind contribution equates to 33 days over 11 months.
Impact Still active
Start Year 2018
 
Description Is it feasible to use mHealth solutions alongside CAM2038 opioid substitution therapy for the treatment of opioid dependence? 
Organisation Camurus AB
Country Sweden 
Sector Private 
PI Contribution Funds from P2D: Time is requested for 3 days per week of an RA for 825 hours to conduct the day-to-day running of the project. A suitable individual has been identified who is currently working in the drug service but has conducted an MSc in psychological research methods having an individual embedded in the service would facilitate the engagement of staff and service users with the project. Funds are requested for the member of staff and PI to visit Monsenso in Copenhagen, where the RA will be seconded for one month adapting the solution for substance use. Permissions have been given for this from the drug service. We also request costs for a laptop for the researcher who will need to travel to various sites of the drug service across Devon, along with costs for participants to attend research visits, backfill for keyworkers The College will provide a match for overheads for the directly allocated staff.
Collaborator Contribution Monsenso will provide access to the relevant software and solution for customising which has had considerable resource for development from numerous EU and Danish grants thus far and the development time to tailor this solution. Camurus will provide match funding towards research costs. Together Drug Services - will provide clinician time in kind and will host the researcher during the data collection phase.
Impact Still active
Start Year 2019
 
Description Is it feasible to use mHealth solutions alongside CAM2038 opioid substitution therapy for the treatment of opioid dependence? 
Organisation Monsenso ApS.
Country Denmark 
Sector Private 
PI Contribution Funds from P2D: Time is requested for 3 days per week of an RA for 825 hours to conduct the day-to-day running of the project. A suitable individual has been identified who is currently working in the drug service but has conducted an MSc in psychological research methods having an individual embedded in the service would facilitate the engagement of staff and service users with the project. Funds are requested for the member of staff and PI to visit Monsenso in Copenhagen, where the RA will be seconded for one month adapting the solution for substance use. Permissions have been given for this from the drug service. We also request costs for a laptop for the researcher who will need to travel to various sites of the drug service across Devon, along with costs for participants to attend research visits, backfill for keyworkers The College will provide a match for overheads for the directly allocated staff.
Collaborator Contribution Monsenso will provide access to the relevant software and solution for customising which has had considerable resource for development from numerous EU and Danish grants thus far and the development time to tailor this solution. Camurus will provide match funding towards research costs. Together Drug Services - will provide clinician time in kind and will host the researcher during the data collection phase.
Impact Still active
Start Year 2019
 
Description Predicting reproductive lifespan 
Organisation Randox Laboratories
Country Global 
Sector Private 
PI Contribution P2D funding: The main cost is for an analyst's time to generate the statistical models. We requested funding for a postdoc in the Complex Traits group with 5 years' experience of working with genetic data and statistical modelling. The College will provide matched in-kind funds in salary costs for the PI, as well as the CoI who has considerable experience of working with Randox already to develop similar models.
Collaborator Contribution Randox will match the funding for this project by evaluating the commercial viability of the predictive test, co-developing the technology necessary to deliver the test and providing consultancy on the project. Randox will arrange for the PDRA to work with their Randox Health partners to evaluate how they currently test for fertility and how a genetic test could be incorporated into their portfolio of tests. This would involve discussing how risk is communicated to customers. Randox would also arrange for the UoE staff member to gain an insight into their genotyping set-up to evaluate how best to develop the genetic aspect of the predictive test. Members of the Randox team will also visit Exeter to help with developing the questions for the qualitative interviews we plan to do. Randox estimate the value of the in-kind contribution to be £20,000.
Impact Still active
Start Year 2018
 
Description Quantifying the efficacy of novel polypeptide therapeutics for Gram-negative infections 
Organisation NPL Ltd
Country United Kingdom 
Sector Private 
PI Contribution Staffing: Funding is requested for the full-time salary of a postdoctoral research associate (grade E26) for 5 months. Consumables: Funding is requested for microfluidic and microfabrication consumables Travel: Funding is requested for two return trips to NPL to monitor progress on the project while the PDRA will be seconded at NPL Staffing: The College will fund the salary of the PI at 20% FTE for the duration of the project and the associated estate and infrastructure costs
Collaborator Contribution Staffing: NPL will cover the salary of the PDRA for 2 months; NPL will also cover the salary of NPL stakeholders who will provide training and supervision during the PDRA secondment at NPL Consumables: NPL will cover the cost of the synthesis of novel antimicrobial peptides
Impact Still active
Start Year 2019
 
Description Chemicals and Environmental Health. Waking up to Planetary Health 2020. Royal Devon and Exeter Hospital and the University of Exeter Medical School. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Third sector organisations
Results and Impact Prof Tyler was invited to be a keynote speaker at the Chemicals and Environmental Health. Waking up to Planetary Health 2020. Royal Devon and Exeter Hospital and the University of Exeter Medical School.
Year(s) Of Engagement Activity 2020
 
Description Delivered a series of talks about antimicrobial resistance both live and online, including some specific tailored for a lay audience 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Delivered a series of talks about antimicrobial resistance both live and online, including some specific tailored for a lay audience
Year(s) Of Engagement Activity 2020
 
Description Endocrine Disrupting Chemicals and Chemicals of Environmental Concern. The 22nd UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Prof Tyler was invited to give the opening address at the Endocrine Disrupting Chemicals and Chemicals of Environmental Concern. The 22nd UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals.
Year(s) Of Engagement Activity 2020
 
Description Exeter Anti Microbial Resistance Workshop (PDRA oral presentation) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Exeter Anti Microbial Resistance Workshop (PDRA oral presentation)
Year(s) Of Engagement Activity 2020
 
Description Fish and amphibian embryos as alternative models in toxicology and teratology, L'Oréal, Aulnay-sous-Bois/Paris, France 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Dr Winter was invited to talk and chair the session on Fish and amphibian embryos as alternative models in toxicology and teratology, L'Oréal, Aulnay-sous-Bois/Paris, France
Year(s) Of Engagement Activity 2020
 
Description Fish and amphibian embryos as alternative models in toxicology and teratology, L'Oréal, Aulnay-sous-Bois/Paris, France (Tyler) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Fish and amphibian embryos as alternative models in toxicology and teratology, L'Oréal, Aulnay-sous-Bois/Paris, France - Detecting and defining drug-induced seizures: the power of functional imaging in the larval zebrafish brain (invited oral presentation and invited session chair) - Matt Winter, 29- 30. November 2019
Year(s) Of Engagement Activity 2019
 
Description Herriot Watt University - Neuropharmacological profiling of chemicals in zebrafish (invited seminar) (Tyler) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Herriot Watt University - Neuropharmacological profiling of chemicals in zebrafish (invited seminar) - Matt Winter, 19th June 2019
Year(s) Of Engagement Activity 2019
 
Description NC3Rs GW4 Show case conference, University of Exeter 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Dr Winter was invited to talk at NC3Rs GW4 Show case conference, University of Exeter
Year(s) Of Engagement Activity 2020
 
Description NC3Rs GW4 Show case conference, University of Exeter (Tyler) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact NC3Rs GW4 Show case conference, University of Exeter - Use of larval zebrafish to replace mammals for seizure liability assessment (invited oral presentation) - Matt Winter, 4th December, 2019
Year(s) Of Engagement Activity 2019
 
Description NIHR Dementia Researcher 'Midday Lectures': Mild behavioural impairment: an emerging concept in cognitive ageing and pre-clinical dementia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Presented at NIHR Dementia Researcher 'Midday Lectures': Mild behavioural impairment: an emerging concept in cognitive ageing and pre-clinical dementia. 14th May 2020.
Year(s) Of Engagement Activity 2020
 
Description Oxford Native Mass Spectrometry Conference 2019 (PDRA poster presentation) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Oxford Native Mass Spectrometry Conference 2019 (PDRA poster presentation)
Year(s) Of Engagement Activity 2019
 
Description Scientists create artificial virus in the fight against superbugs. The Times and The Daily Mail articles, with interview. 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Scientists at NPL, working with partners from the University of Cambridge, University of Exeter, King's College London and University College London have developed a mechanism of antibacterial persistence to combat persistent and resistant bacterial infections.

The rise of superbugs is a serious concern in the medical community as bacteria evolve to evade existing treatments faster than new antibiotics can be developed. Rather than seeking out antibiotics that exist in nature, as has been the case with previous advances, the team of experts have designed one from the ground up, inspired by viruses.

As featured in The Times and the Daily Mail today, Maxim Ryadnov, Area Science Leader at NPL said: "Viruses are geometric objects. They are like solid cages built from tiny blocks glued together with an atomistic precision. We take that shape, strip off their viral proteins, and are left with a template."

To pursue such a feat, this interdisciplinary research team adopted the geometric principles of the virus architecture to engineer a synthetic biologic - protein ?-capsid - which assembles from a small molecular motif found in human cells. This motif can recognize pathogen-associated molecular patterns on bacterial surfaces but by itself is weakly antimicrobial. By contrast, each capsid, which comprises multiple copies of the motif, delivers an influx of high antimicrobial doses in its precise binding position on a bacterial cell.

Using a combination of nanoscale and single-cell imaging the team demonstrated that the capsids inflict irreparable damage to bacteria, rapidly converting into nanopores in their membranes and reaching intracellular targets. The capsids were equally effective in either of their chiral forms, which can render them invisible to the immune system of the host, killing different bacteria phenotypes and superbugs without cytotoxicity in vitro and in vivo.

At UCL, the scientists visualised how the capsids landed on their targets and next created nanometre-size holes, which ultimately are lethal to the bacteria. According to Katharine Hammond, research scientist at NPL and PhD student at UCL: "By scanning a sharp tip over the membrane surface, just like a miniature finger would read Braille, we could trace the contours of the capsids on the membranes and observe in real time how they punctured holes in their target membranes."

Ibolya Kepiro, Higher Research Scientist, National Physical Laboratory (NPL) states: "This research culminates our joint efforts to identify an antibacterial mechanism that could be free from the frustration of bacterial persistence. We believe that these findings hold promise for the systemic assessment of antimicrobial efficacy".

The findings are reported in ACS Nano and demonstrate how bioengineering and multi-modal measurements can offer and validate innovative solutions to healthcare, building on natural disease-fighting capabilities.
Year(s) Of Engagement Activity 2019,2020
URL https://www.thetimes.co.uk/article/man-made-antibacterial-virus-offers-hope-in-superbug-fight-0pv25l...
 
Description Seimnar at Herriot Watt University - Neuropharmacological profiling of chemicals in zebrafish 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Dr Winter was invited to a seminar- Neuropharmacological profiling of chemicals in zebrafish at Herriot Watt University
Year(s) Of Engagement Activity 2020
 
Description Seminar Brunel University: Neuropharmacological profiling in the larval zebrafish brain 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Brunel University: Neuropharmacological profiling in the larval zebrafish brain
Year(s) Of Engagement Activity 2020
 
Description South West Zebrafish Meeting University of Bristol 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Prof Tyler and Dr Winter were invited to talk at the South West Zebrafish Meeting University of Bristol
Year(s) Of Engagement Activity 2020
 
Description South West Zebrafish Meeting University of Bristol (Tyler) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact South West Zebrafish Meeting University of Bristol - Detecting and defining drug-induced seizures: the power of functional imaging in the larval zebrafish brain (oral presentation) - Matt Winter, 9th September 2019
Year(s) Of Engagement Activity 2019
 
Description The Royal Society Industry Fellows networking event. Exploiting the power of CRISPR/Cas9 gene editing for industry-standard drug target validation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Dr Winter was invited to talk at The Royal Society Industry Fellows networking event. Exploiting the power of CRISPR/Cas9 gene editing for industry-standard drug target validation
Year(s) Of Engagement Activity 2020
 
Description The Royal Society Single cell ecology - Discussion Meeting, 10-11 December 2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Interdisciplinary meeting to explore the use of single cell technologies to understand the function, diversity and interactions of microbes. By bringing together physicists who manipulate cells, microbiologists who seek to understand the nature of microbial communities and genomicists who are developing new approaches to study individual cells we will achieve a greater understanding of the potential of this new field.

15:30-16:00
Single-cell responses to environmental cues
Dr Stefano Pagliara, University of Exeter, UK. Clonal microbial populations feature cell-to-cell differences in physiological parameters such as gene expression, growth rate, and resistance to stress. This phenotypic heterogeneity is at the basis of fundamental biological processes such as membrane transport, stem cell differentiation, and tolerance to antimicrobial compounds. Therefore, it is paramount understanding how changes in the environment affect the phenotypic heterogeneity within a clonal microbial population. Stefano will illustrate how embryonic stem cells respond to physical environmental cues such as transient confinement into narrow grooves. He will show that this mechanical response is heterogeneous. Remarkably, the nuclei of some embryonic stem cells display a unique material property that is they are auxetic; exhibiting a cross-sectional expansion when stretched and a cross-sectional contraction when compressed. He will then talk about the different strategies that individual bacteria can exploit to escape antibiotics and illustrate current efforts to understand how the environment predetermines the outcome of antibiotic treatment.
Year(s) Of Engagement Activity 2018
URL https://royalsociety.org/science-events-and-lectures/2018/12/single-cell/