The role of p53/p73 family members in the cytotoxic response.

Lead Research Organisation: MRC Toxicology Unit

Abstract

Many of the drugs used in cancer therapy, exert their action through the activation of the p53-family members. Indeed, loss of p53 function in cancer is associated with poor prognosis and resistance to chemotherapy. Moreover, this family of transcription factors play a key role in development of central nervous system, epidermal tissue and in overall organism metabolism. Using genetically modified mouse model and high throughput analysis, we aim to understand:
1) How these transcription factors mediate chemo sensitivity,
2) How they regulate organismal development and physiology.

Technical Summary

The scope of the Apoptosis & Cancer Laboratory is to understand the fundamental mechanisms of cell death (apoptosis) and tissue response to injury caused by drugs, chemicals and endogenous molecules, like free radicals. In particular, we study the physiological functions of the p53 family proteins (namely p53, p63 and p73) and their involvement in tumorigenesis and neurodegeneration triggered by toxic insults. We make use of multiple scientific approaches ranging from systems biology (proteomic and genomic screenings) to the generation and characterization of genetically modified mouse models.
We are also interested to the development of chemical compounds that can regulate expression and function of these transcription factors, and that could therefore have potential therapeutic applications.

Objectives
Alterations of proteins that control cell proliferation, cell survival and cell death are the main causes of diseases such as cancer and neurodegenerative disorders and dictate the efficacy of drug treatment. The protein members of the p53 family, composed of TP63, TP73 and TP53 itself, are transcription factors that, by binding to the DNA, modulate the expression of multiple genes that play pivotal functions in the regulation of proliferation, differentiation, cell death, stem cell renewal and cell fate commitment. While p53 has been extensively studied over the last 32 years, p73 biology is still lagging behind despite lying at the crossroads between differentiation, cell cycle control and cell death.

We aim to understand the mechanisms of p73 regulation during toxic insults and to define how it influences the outcome and severity of cellular and organism response to toxic insults. To this end, we are investigating several aspect of the p53-family biology, including:

1- p73 function in DNA damage response and cancer therapy,
2- Characterization of in vivo DNA damage models & large-scale transcriptional analysis,
3- p73, metabolism and ageing,
4- Neuronal implications of p73 deficiency,
5-Bioinformatic Evaluation of predictive value of p53 family in cancer progression and chemosensitivity in collaboration with Alexej Antonov (MRC Toxicology Unit),
6- MicroRNA and the p53 family in cancer progression in collaboration with Martin Bushell (MRC Toxicology Unit),
7- The deregulation of translation, in collaboration with Anne E. Willis (MRC Toxicology Unit),
8- Function and Pharmacological inhibition of the p73 E3 ubiquitin ligase ITCH.

Publications

10 25 50
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Aberdam D (2008) miRNAs, 'stemness' and skin. in Trends in biochemical sciences

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Agostini M (2011) Cell death pathology: perspective for human diseases. in Biochemical and biophysical research communications

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Agostini M (2011) Neuronal differentiation by TAp73 is mediated by microRNA-34a regulation of synaptic protein targets. in Proceedings of the National Academy of Sciences of the United States of America

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Agostini M (2016) Metabolic reprogramming during neuronal differentiation. in Cell death and differentiation

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Agostini M (2014) miR-34: from bench to bedside. in Oncotarget

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Agostini M (2010) p73 regulates maintenance of neural stem cell. in Biochemical and biophysical research communications

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Agostini M (2018) The p53 Family in Brain Disease. in Antioxidants & redox signaling

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Agostini M (2011) microRNA-34a regulates neurite outgrowth, spinal morphology, and function. in Proceedings of the National Academy of Sciences of the United States of America

 
Description Consiglio Nazionale Ricerche (Italian Research Council)-Advisor
Geographic Reach Europe 
Policy Influence Type Participation in advisory committee
Impact Scientific advisor to the Headoffice
 
Description European Brain Research Institute (Rome, Italy)
Geographic Reach Europe 
Policy Influence Type Participation in advisory committee
Impact Scientific Advisor
 
Description Science Foundation of Ireland
Geographic Reach Europe 
Policy Influence Type Participation in advisory committee
Impact Scientific advisor
 
Description University of Saint Petersburg, Russia
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
Impact Supervise the development and organisation of the scientific laboratory in University Saint Petersburg in Russia. Sponsored by the Ministry of Education and Science of the Russian Federation.
 
Description VIB, biotech Research Institute of the Belgium Government
Geographic Reach Europe 
Policy Influence Type Participation in advisory committee
Impact Scientific Advisor to the VIB in Ghent, Belgium
 
Title Antibody for p73 
Description Generation of a series of Ab specific for different isoforms of p73, both in mouse and man. 
Type Of Material Antibody 
Year Produced 2008 
Provided To Others? Yes  
Impact Useful reagents, not available commercially, to study p73 in development and cancer. 
 
Title DNp73 knockout mice 
Description New knockout mice deleting DNp73 protein. 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2009 
Provided To Others? Yes  
Impact KO prepared in collaboration with Tak W. Mak (Toronto). Models of cancer development following DNA damage in mice. 
 
Title FLASH Knockout mice 
Description Production of knockout mice for FLASH, a protein component of the Cajal body in the cell nucleous, regulating cell cycle progression. 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Provided To Others? No  
Impact Understanding the progression in cell cycle and the histone regulation 
 
Title TAp73 knockout mice 
Description Gene disruption of TAp73. 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2008 
Provided To Others? Yes  
Impact Transgenic mice prepared in collaboration with Tak W. Mak (Toronto). Models of cancer development following DNA damage in mice. 
 
Title Trp63D13 Knockout mice 
Description knockout mice for SAM-domain containing isoforms of p63 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Provided To Others? No  
Impact Model for cancer studies 
 
Title Trp73D13 knockout mice 
Description Knockout mice for SAM-domain containing isoforms of p73 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Provided To Others? No  
Impact Model for cancer studies 
 
Title USP47 knockout mice 
Description Gene disruption of the deubiquitinase USP47 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Provided To Others? No  
Impact Substrate of b-Trcp. Potential model of cancer in mice. 
 
Title ZNF750 knockout mice 
Description Genetic deletion of ZNF750 gene (conditional knockout mouse) 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Provided To Others? No  
Impact Potentially useful for cancer and skin development studies 
 
Title p73 & p63 inducible cell lines 
Description Tet-on inducible TAp73, DNp73, TAp63, DNp63 SAOS cell lines. 
Type Of Material Cell line 
Year Produced 2006 
Provided To Others? Yes  
Impact Our in vitro models are currently used by dozen of laboratories in EU and USA 
 
Description DNp73 knockout mice 
Organisation University of Toronto
Country Canada 
Sector Academic/University 
PI Contribution Univ Toronto in close collaboration with our MRC group has developped, charaterized and stidied the KO mice of the gene DNp73, involved in DNA damage response.
Collaborator Contribution Development, production and characterization of DNp73 mice to evaluate the role of this gene in DNA damage response.
Impact Production of joint scientific publications. Understanding of the molecuar mechanisms in Cancer and toxicity.
Start Year 2006
 
Description E3 Ubiquitin Inhibitors 
Organisation MRC-Technology
Department MRCT Centre for Therapeutics Discovery (CTD)
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Set up robotized test for HTS. Validated targets found. Confirmed specificity of inhibitors.
Collaborator Contribution Performed Highthrouput screening for ITCH inhibitors Validated positive hits. Confirmed inhibitors
Impact Development of in hibitors of the E3 ubiquitin ligase ITCH for cancer therapy. The work alloes a collaboration with top scientists (Chemistry Nobel Laureate) to validate the inhibitors. A further collaboration has been established with high profile, structural biology at the University of Frankfurt, to characterise the inhibitor/ITCH structure.
Start Year 2006
 
Description Itch Inhibitors Screening 
Organisation Goethe University Frankfurt
Department Structural Biology Research Area
Country Germany 
Sector Academic/University 
PI Contribution Discussion on Methods and protocols.
Collaborator Contribution Develop biochemical techniques to evaluate the activity and specificity of the inhibitors identified by MRC-T collaboration against ITCH.Evaluation of the efficacy of ITCH inhibitors in vitro on cancer cell linesResolution of the crystal structure of ITCH, and of the ITCH+inhibitor
Impact Development of High Throughput Screening for the E3 ubiquitin ligase ITCH and other members of the same HECT family. Currently the validation of the lead compounds is in progress.
Start Year 2007
 
Description Itch Inhibitors Screening 
Organisation Technion - Israel Institute of Technology
Department Department of Biochemistry
Country Israel 
Sector Academic/University 
PI Contribution Discussion on Methods and protocols.
Collaborator Contribution Develop biochemical techniques to evaluate the activity and specificity of the inhibitors identified by MRC-T collaboration against ITCH.Evaluation of the efficacy of ITCH inhibitors in vitro on cancer cell linesResolution of the crystal structure of ITCH, and of the ITCH+inhibitor
Impact Development of High Throughput Screening for the E3 ubiquitin ligase ITCH and other members of the same HECT family. Currently the validation of the lead compounds is in progress.
Start Year 2007
 
Description Itch Inhibitors Screening 
Organisation University of Naples
Department Biochemistry Department
Country Italy 
Sector Academic/University 
PI Contribution Discussion on Methods and protocols.
Collaborator Contribution Develop biochemical techniques to evaluate the activity and specificity of the inhibitors identified by MRC-T collaboration against ITCH.Evaluation of the efficacy of ITCH inhibitors in vitro on cancer cell linesResolution of the crystal structure of ITCH, and of the ITCH+inhibitor
Impact Development of High Throughput Screening for the E3 ubiquitin ligase ITCH and other members of the same HECT family. Currently the validation of the lead compounds is in progress.
Start Year 2007
 
Description Itch silencing 
Organisation Ectopharma Ltd
Country United Kingdom 
Sector Private 
PI Contribution Material Transfer Agreement - oncology application of Itch silencing in cancer
Collaborator Contribution Validation of ITCH inhibition as therapeutic target in cancer cells. Validation was performed both in vitro and in vivo.
Impact Development and validation of shRNA silencing against the E3 ubiquitin ligase ITCH for therapy of p73-dependent tumours.
Start Year 2006
 
Description TAp73 knockout mice 
Organisation University of Toronto
Country Canada 
Sector Academic/University 
PI Contribution Preparation of construct, Homologous recombination in ES cells. Developemt of the KO mice. Characterization of the phenotype
Collaborator Contribution Toronto, jointly produced KO mice for TAp73 gene, involved in DNA damage response.
Impact Information on the molecular mechanisms of DNA damage repair systems. Production of joint scientific publications
Start Year 2006
 
Description p53 family transcription 
Organisation Metabolon Inc
Department Research and Development Metabolon
Country United States 
Sector Private 
PI Contribution Identify target genes of the transcription factors p53/p63/p73, relevant in Cancer and Neurodegeneration. Involve DNA microarray, ChIP, luc-assay, trasncritional regulation. Experiments are both in vitro and in vivo. Specific interest is for genes regulating Metabolism, which is evaluated by Mass Spectorometric metabolomics.
Collaborator Contribution DNA array profiling, ChIP analysis, Gene promoter studies.Metabolite identification by Mass Spectrometry.Transcription analysis by ChIP, array. Metabolic profiling by Mass Spectrometry.
Impact Scientific Publications. Genes regulating metabolism. Toxic insults affecting metabolism via p53 family.
Start Year 2011
 
Description p53 family transcription 
Organisation Saint Petersburg State University
Country Russian Federation 
Sector Academic/University 
PI Contribution Identify target genes of the transcription factors p53/p63/p73, relevant in Cancer and Neurodegeneration. Involve DNA microarray, ChIP, luc-assay, trasncritional regulation. Experiments are both in vitro and in vivo. Specific interest is for genes regulating Metabolism, which is evaluated by Mass Spectorometric metabolomics.
Collaborator Contribution DNA array profiling, ChIP analysis, Gene promoter studies.Metabolite identification by Mass Spectrometry.Transcription analysis by ChIP, array. Metabolic profiling by Mass Spectrometry.
Impact Scientific Publications. Genes regulating metabolism. Toxic insults affecting metabolism via p53 family.
Start Year 2011
 
Description p53 family transcription 
Organisation University of Rome Tor Vergata
Department Department of Experimental Medicine and Surgery
Country Italy 
Sector Academic/University 
PI Contribution Identify target genes of the transcription factors p53/p63/p73, relevant in Cancer and Neurodegeneration. Involve DNA microarray, ChIP, luc-assay, trasncritional regulation. Experiments are both in vitro and in vivo. Specific interest is for genes regulating Metabolism, which is evaluated by Mass Spectorometric metabolomics.
Collaborator Contribution DNA array profiling, ChIP analysis, Gene promoter studies.Metabolite identification by Mass Spectrometry.Transcription analysis by ChIP, array. Metabolic profiling by Mass Spectrometry.
Impact Scientific Publications. Genes regulating metabolism. Toxic insults affecting metabolism via p53 family.
Start Year 2011
 
Description p73 degradation and microRNA 
Organisation German Centre for Neurodegenerative Diseases
Country Germany 
Sector Public 
PI Contribution Characterization of novel pathway of degradation of p73, independent of ITCH. Characterization of the miR regulating p73 function.
Collaborator Contribution Allowed the collaboration on two areas: (i) p73 degradation, (ii) miR related to p73Analysis of the miR profile from invitro and in vivo samples to evaluate the fine regulation of p73 and p63 pathwaysAnalysis of murine ES cell committment and the p73 pathwaypreparation of the miR-34a (as a target of p73) knockout and its characterization
Impact Exchange of reagents, cooperation on experiments, exchange of personnel, production of joint scientific publications
 
Description p73 degradation and microRNA 
Organisation University of Nice Sophia-Antipolis
Department Cell Biology
Country France 
Sector Academic/University 
PI Contribution Characterization of novel pathway of degradation of p73, independent of ITCH. Characterization of the miR regulating p73 function.
Collaborator Contribution Allowed the collaboration on two areas: (i) p73 degradation, (ii) miR related to p73Analysis of the miR profile from invitro and in vivo samples to evaluate the fine regulation of p73 and p63 pathwaysAnalysis of murine ES cell committment and the p73 pathwaypreparation of the miR-34a (as a target of p73) knockout and its characterization
Impact Exchange of reagents, cooperation on experiments, exchange of personnel, production of joint scientific publications
 
Description p73 degradation and microRNA 
Organisation University of Rome Tor Vergata
Country Italy 
Sector Academic/University 
PI Contribution Characterization of novel pathway of degradation of p73, independent of ITCH. Characterization of the miR regulating p73 function.
Collaborator Contribution Allowed the collaboration on two areas: (i) p73 degradation, (ii) miR related to p73Analysis of the miR profile from invitro and in vivo samples to evaluate the fine regulation of p73 and p63 pathwaysAnalysis of murine ES cell committment and the p73 pathwaypreparation of the miR-34a (as a target of p73) knockout and its characterization
Impact Exchange of reagents, cooperation on experiments, exchange of personnel, production of joint scientific publications
 
Description p73 degradation and microRNA 
Organisation University of Texas
Department M. D. Anderson Cancer Center
Country United States 
Sector Academic/University 
PI Contribution Characterization of novel pathway of degradation of p73, independent of ITCH. Characterization of the miR regulating p73 function.
Collaborator Contribution Allowed the collaboration on two areas: (i) p73 degradation, (ii) miR related to p73Analysis of the miR profile from invitro and in vivo samples to evaluate the fine regulation of p73 and p63 pathwaysAnalysis of murine ES cell committment and the p73 pathwaypreparation of the miR-34a (as a target of p73) knockout and its characterization
Impact Exchange of reagents, cooperation on experiments, exchange of personnel, production of joint scientific publications
 
Title DNp73 & p63 phosphorylation by c-Abl 
Description The kinase c-Abl phosphorilated p73/p63 to regulate their function. In oocytes, p63 phosphorylation is crucial for their survival during chemotherapy. Gleevec (c-Abl inhibitor) protects from infertility following chemotherapy. 
IP Reference  
Protection Protection not required
Year Protection Granted 2009
Licensed No
Impact This application relates to identification of the human sequence of Delta Np73 (including the promoter region) and manipulation of Delta Np73 expression/activity for treatment and diagnostics of cancer Patent filed by and managed by Eirex Therapeutics as a result of collaboration
 
Title Itch-p73 Interaction 
Description Identification of the mechanism of degradation of p73 by the ubiquitin E£ ligase ITCH 
IP Reference US2009306187 
Protection Patent application published
Year Protection Granted 2009
Licensed Yes
Impact This patent identifies Itch, an ubiquitin ligase, as a novel regulator of the p53 family of tumour suppressors. The claims cover screens to identify modulators of Itch and use of Itch modulators as treatments for cancer. National phase
 
Description CDD-Retreat. Capri, Italy. (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact All the talks provoked interesting discussions

The meeting was attended by high-profile scientist in cancer biology field
Year(s) Of Engagement Activity 2014
 
Description "Cell Death in Disease". Saint Petersburg Institute Technology. Saint Petersburg, Russia. (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description "Challenges and Solutions in Cancer Research and Treatment" Angra dos Reis, Brasil 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended and provoked several questions

The talk provoked several questions
Year(s) Of Engagement Activity 2014
 
Description "Molecular mechanisms of cancer". C.N.R. Napoli, Italy 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description "Pancreatic Cancer", Ravello, Italy 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk provoked several interesting questions

A possible collaboration with clinicians has been discussed.
Year(s) Of Engagement Activity 2014
 
Description "Proteosomal Degradation of Proteins". Saint Petersburg Institute Technology. Saint Petersburg, Russia (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation paper presentation
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The meeting was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description 10th Weinmann Symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Scientific Conference
Year(s) Of Engagement Activity 2017
 
Description 13th International Summer School on "Inflammation, Immunomodulation, Inspiration". Bern, Swiss 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The talk provoked several interesting questions

Several PhD students have asked if they could visit my lab
Year(s) Of Engagement Activity 2014
 
Description 1st CDDIS & 4th Sino British Workshop on Cell Death. Shanghai, China 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended and provoked several questions

It has been of interest to the range of participants in the publishing process: from less experienced authors to those undertaking peer review.
Year(s) Of Engagement Activity 2013
 
Description 21st ECDO EuroConference on Cell Death. Paris, France 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended


The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description 22nd ECDO EuroConference on Cell Death. Crete, Grece. 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2014
 
Description 25th Pezcoller Symposium. "Metabolism and Tumorigenesis". Trento, Italy. 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended and provoked several questions

Throughout the meeting, several new ideas were formulated and a potential collaboration was discussed.
Year(s) Of Engagement Activity 2013
 
Description 2nd EACR Special Conference: Cell Death in Cancer. Amsterdam, Netherland. 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended




The talk provoked several interesting questions
Year(s) Of Engagement Activity 2014
 
Description 31st CARD, COngres Annuel Recherche Dermatologique. Paris, France 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description 31st CARD, COngres Annuel Recherche Dermatologique. Paris, France. 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended



The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description 3rd CDDIS & 6th Sino British Workshop on Cell Death. Shanghai, China 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk provoked several interesting questions


It has been of interest to the range of participants in the publishing process: from less experienced authors to those undertaking peer review.
Year(s) Of Engagement Activity 2014
 
Description 6th International Mutant p53 Workshop. Toronto, Canada 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description 6th p63/p73 International Workshop. Kazusa Akademia Park in Chiba, Japan (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

Several potential collaboration have been discussed
Year(s) Of Engagement Activity 2013
 
Description 8th Tuscany Retreat on Cancer Research and Apoptosis. ,,Genetic profiling, resistance mechanism and novel treatment concepts in cancer. Italy 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation paper presentation
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The talk provoked several interesting questions

Several PhD asked to visit my lab
Year(s) Of Engagement Activity 2013
 
Description Advanced Course on "Cancer Therapeutics". Saint Petersburg Institute Technology. Saint Petersburg, Russia (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The meeting was very well attended

Al the talks provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description Ageing Workshop "Aviesan". Paris, France 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description Biochemical Journal Workshop. London 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended



The talk provoked several questions
Year(s) Of Engagement Activity 2014
 
Description CDD-Retreat. Capri, Italy (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The meeting was attended by well-known scientists in the field of cancer biology

The talk was very well attended and provoked several questions
Year(s) Of Engagement Activity 2013
 
Description COMBIO 2013. Perth, Australia 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description Cell Processes: Life and death decisions". Biochemical Journal Symposium. Tsinghua University, Beijing. China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended and provoked several questions

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description EEC-ectodermal dysplasia and syndromes". Padova, Italy 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk provoked an interesting discussion

A potential collaboration was discussed
Year(s) Of Engagement Activity 2013
 
Description EMBO Workhop "Death in the Alps" (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2013
 
Description FEBS Publication Committee, Budapest 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact FEBS Publications Committee oversees the operation and development of FEBS' journals.
Year(s) Of Engagement Activity 2014
 
Description Gordon Research Conference on "Cell Death". Mount Snow Resort, Vermont. 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2014
 
Description International p53 Workshop. Stockholm, Sweden 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk provoked several interesting questions

Results of a previous collaboration have been didcussed
Year(s) Of Engagement Activity 2014
 
Description Keystone Symposium on "Cell Death Signaling in Cancer and the Immune System". Guaruja, São Paulo, Brasil. 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended

The talk provoked several interesting questions
Year(s) Of Engagement Activity 2014
 
Description Mini-Workshop on "Science Integrity" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Organisation of Mini-workshop focusing Science Integrity including talks and debate with scientists, journal editors and publishers.
Year(s) Of Engagement Activity 2016
 
Description National Congress of Biochemistry and Molecular Biology, Xiamen, China (Keynote Lecture) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The lecture was very well attended and provoked several questions

The lecture provoked an interesting discussion
Year(s) Of Engagement Activity 2014
 
Description Therapeutic Targets for Cell Death in Cancer, Pharmacy Summer School; Rome, Italy. (Organizer, Chair) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The talk provoked several interesting questions

Several PhD students asked to visit my lab
Year(s) Of Engagement Activity 2013
 
Description Winter School "Cell death & cancer" Obergurgl, Innsbruck, Austria. (Keynote Lecture) 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk was very well attended



The talk provoked an interesting discussion
Year(s) Of Engagement Activity 2014
 
Description Workshop on Tumour Immunology. Toronto 
Form Of Engagement Activity Scientific meeting (conference/symposium etc.)
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk provoked several interesting questions

A potential collaboration has been discussed
Year(s) Of Engagement Activity 2014
 
Description p53 International Meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact International topical conference
Year(s) Of Engagement Activity 2017