The Enteroendocrine System in Health and Disease

Lead Research Organisation: University of Cambridge

Abstract

The aims of our research are to understand how hormones from the gut influence appetite and blood glucose control.
After a meal, the gut produces hormones signalling to the brain to stop eating, and to the pancreas that it is time to release more insulin. One of these gut hormones has recently been developed as a new treatment for diabetes, and many people are interested in whether any other gut hormones could also be developed for the treatment of diabetes and obesity.
Our programme of research aims to understand how gut hormones are released after a meal, and whether it would be possible to alter their production as a new form of treatment. We study how individual hormone-producing cells detect nutrients, using techniques that allow us to monitor how single cells respond to stimuli in real time. By removing specific genes in mice, we can build up a picture of which genes are most important for how these gut cells work. The findings can then be used as a starting point for devising new ways to alter hormone release in humans.

Technical Summary

This programme addresses enteroendocrine cell physiology, identifying strategies to enhance secretion of gut hormones that regulate appetite and insulin release.
The gut endocrine system is increasingly recognised as a central player in the coordination of appetite, insulin release and post-prandial nutrient homeostasis. It likely underlies many of the beneficial physiological responses to gastric bypass surgery, and some of the secreted hormones have already been harnessed successfully for the treatment of diabetes and short bowel syndrome. Emerging therapies based on the secretion and action of gut peptides are predicted to be major players in the future treatment of metabolic disease.
Understanding the physiology of the gut endocrine system is critical for identifying novel strategies to modulate hormone release in humans and interpreting post-ingestive and post-bypass physiology. This is an area of research that has advanced dramatically in the past decade, with the introduction of technologies to identify, isolate and interrogate enteroendocrine cells in murine models. As a result, some general principles of stimulus detection in incretin-secreting K- and L-cells have now been established, but there are many unanswered questions such as the relative importance of different enteroendocrine signalling pathways in vivo, the functional overlap between different enteroendocrine cell types, and the impact of genetics, diet and metabolic disease on the enteroendocrine system?
These questions will be addressed here using a candidate gene approach. Genes for functional evaluation will be selected based on their predicted effects on whole body physiology, enteroendocrine cell development, nutrient sensing and/or secretion. State-of-the-art optical and electrophysiological recording techniques will be utilised to monitor dynamic physiological responses of single identified enteroendocrine cells in primary culture, and the intact gut-brain-pancreatic axis will be assessed in vivo using genetically modified mouse models. Promising enteroendocrine target receptors and pathways will be further evaluated for translational effects on appetite and glucose homeostasis in humans.

Organisations

Publications

10 25 50
 
Description JDRF UK Beta Cell Renewal Roundtable
Geographic Reach National 
Policy Influence Type Participation in advisory committee
 
Description MRC Workshop on Neuroscience of Obesity: Gut-brain communication
Geographic Reach National 
Policy Influence Type Participation in advisory committee
 
Description Addenbrooke's Charitable Trust Fellowship (Geoff Roberts)
Amount £52,000 (GBP)
Organisation Addenbrooke's Charitable Trust (ACT) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2016 
End 09/2017
 
Description BBSRC DTP PhD studentship (Ming Yang)
Amount £95,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2015 
End 09/2019
 
Description BBSRC FTMA to Juraj Rievaj
Amount £14,656 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 07/2018
 
Description BBSRC iCASE
Amount £105,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description BBSRC iCASE
Amount £145,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description BBSRC iCASE studentship partnered with MedImmune (Sam Galvin)
Amount £95,196 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2016 
End 09/2020
 
Description Bakala Foundation Scholarship (Cristian Riccio)
Amount £15,000 (GBP)
Organisation Zdenek and Michaela Bakala foundation 
Sector Charity/Non Profit
Country Switzerland
Start 08/2014 
End 07/2015
 
Description British Society for Neuroendocrinology small grant (Juraj Rievaj)
Amount £3,500 (GBP)
Organisation British Society for Neuroendocrinology 
Sector Learned Society
Country United Kingdom
Start 04/2017 
End 06/2017
 
Description DAAD-PROMOS-Stipend for Ms Friederike Uhlig
Amount € 1,725 (EUR)
Organisation University of Hohenheim 
Sector Academic/University
Country Germany
Start 09/2013 
End 12/2013
 
Description EASD travel grant
Amount € 300 (EUR)
Organisation European Association for the Study of Diabetes (EASD) 
Sector Charity/Non Profit
Country European Union (EU)
Start 10/2018 
End 10/2018
 
Description EFSD
Amount € 122,000 (EUR)
Funding ID rg89241 
Organisation European Association for the Study of Diabetes (EASD) 
Department European Foundation for the Study of Diabetes (EFSD)
Sector Charity/Non Profit
Country Germany
Start 06/2017 
End 11/2018
 
Description EFSD/Boehringer Ingelheim Programme
Amount € 100,000 (EUR)
Organisation European Association for the Study of Diabetes (EASD) 
Department European Foundation for the Study of Diabetes (EFSD)
Sector Charity/Non Profit
Country Germany
Start 08/2014 
End 07/2016
 
Description EMBO Short Term Fellowship (Catalin Chimerel)
Amount € 7,500 (EUR)
Organisation European Molecular Biology Organisation 
Sector Learned Society
Country European Union (EU)
Start 09/2016 
End 12/2016
 
Description Early career grant to Juraj Rievaj
Amount £8,588 (GBP)
Organisation Society for Endocrinology 
Sector Learned Society
Country United Kingdom
Start 09/2017 
End 06/2018
 
Description Early career grant to Van Lu
Amount £10,000 (GBP)
Organisation Society for Endocrinology 
Sector Learned Society
Country United Kingdom
Start 08/2017 
End 07/2018
 
Description G/R, Joint Wellcome Trust Senior Investigators
Amount £1,900,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2015 
End 06/2020
 
Description Lister Summer Studentship
Amount £1,600 (GBP)
Organisation Lister Institute of Preventive Medicine 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2013 
End 08/2013
 
Description MedImmune PhD studentship programme
Amount £96,074 (GBP)
Organisation MedImmune 
Sector Private
Country United States
Start 10/2014 
End 09/2018
 
Description Medimmune grant
Amount £272,857 (GBP)
Organisation MedImmune 
Sector Private
Country United States
Start 06/2015 
End 08/2017
 
Description Project Support Grant to Juraj Rievaj
Amount £3,680 (GBP)
Organisation British Society for Neuroendocrinology 
Sector Learned Society
Country United Kingdom
Start 03/2017 
End 03/2018
 
Description Royal College of Surgeons Research Fellowship
Amount £20,000 (GBP)
Organisation Royal College of Surgeons of England 
Sector Learned Society
Country United Kingdom
Start 09/2017 
End 09/2018
 
Description Society for Endocrinology Early Career Grant (Arianna Psichas)
Amount £10,000 (GBP)
Organisation Society for Endocrinology 
Sector Learned Society
Country United Kingdom
Start 03/2015 
End 03/2016
 
Description Society for Endocrinology Early Career Grant (Catalin Chimerel)
Amount £9,924 (GBP)
Organisation Society for Endocrinology 
Sector Learned Society
Country United Kingdom
Start 01/2017 
End 05/2017
 
Title G/R, Measurement of GLP-1 from epithelial sheets 
Description Optimising the use of Ussing chambers technology for the reliable measurement of basolateral GLP-1 secretion from epithelial sheets 
Type Of Material Technology assay or reagent 
Year Produced 2015 
Provided To Others? Yes  
Impact Method allowed to distinguish between apical and basolateral effect of various drugs on GLP-1 secretion (Brighton et al, 2015) 
URL http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606749/
 
Title GIP-Venus transgenic mouse 
Description Venus driven by the GIP promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2009 
Provided To Others? Yes  
Impact Shared with some other groups and licensed 
 
Title GLU-Cre transgenic mouse 
Description Cre-recombinase driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2012 
Provided To Others? Yes  
Impact Shared with other groups and licensed to industry 
 
Title GLU-Venus transgenic mouse 
Description Fluorescent protein driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2008 
Provided To Others? Yes  
Impact Shared with many collaborators around the world and licensed to industry 
 
Title Glu-CreERT2 
Description Tamoxifen-inducible cre expression driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2017 
Provided To Others? Yes  
Impact Shared with academic collaborators and licensed to industry 
 
Title Glu-Epac2camps transgenic mouse 
Description transgenic mice in which the cAMP FRET sensor Epac2camps is driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2016 
Provided To Others? Yes  
Impact Shared with collaborators 
 
Title sst-cre transgenic mouse 
Description Cre-recombinase driven by the somatostatin promoter in a transgenic mouse model 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2015 
Provided To Others? Yes  
Impact Shared with academic collaborators 
 
Description Acosta 
Organisation Mayo Clinic
Country United States 
Sector Charity/Non Profit 
PI Contribution Training provided to Acosta's group
Collaborator Contribution Analysis of patient samples
Impact None
Start Year 2018
 
Description Brubaker lab 
Organisation University of Toronto
Country Canada 
Sector Academic/University 
PI Contribution Mouse models and discussion
Collaborator Contribution Experimentation and project design
Impact Publication
Start Year 2015
 
Description FG, Susan Jebb, HNR 
Organisation Medical Research Council (MRC)
Department MRC Human Nutrition Research Unit
Country United Kingdom 
Sector Public 
PI Contribution Assays and discussion
Collaborator Contribution Ran project
Impact Publication, PMID: 26054049
Start Year 2014
 
Description Full4Health 
Organisation Danone
Country France 
Sector Private 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Encap Drug Delivery
Country United Kingdom 
Sector Private 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Harokopio University
Country Greece 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Maastricht University (UM)
Country Netherlands 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Norwegian University of Science and Technology (NTNU)
Country Norway 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University Medical Center Utrecht (UMC)
Country Netherlands 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Aberdeen
Country United Kingdom 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Cologne
Country Germany 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Gothenburg
Country Sweden 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Leeds
Country United Kingdom 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Lille
Country France 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Wageningen
Country Netherlands 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description G/R Bertie Goettgens, Single Cell RNAseq 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Experiments
Collaborator Contribution Experiments
Impact none yet
Start Year 2015
 
Description G/R, Bart Staels, FXR 
Organisation University of Lille
Country France 
Sector Academic/University 
PI Contribution Experiments and data
Collaborator Contribution Experiments
Impact Publication, PMID: 26134028
Start Year 2014
 
Description G/R, Eelco de Koning, gut organoids 
Organisation Utrecht University
Country Netherlands 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice) Publications: PMIDs: 25500886, 24130334
Collaborator Contribution Experiments
Impact Publications: PMIDs: 25500886, 2413033
Start Year 2013
 
Description G/R, Fredrik Backhed, Germ free/ gut hormones 
Organisation University of Gothenburg
Country Sweden 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Provision of germ free animals / experiments
Impact none as yet
Start Year 2015
 
Description G/R, Full4 Health 
Organisation Harokopio University
Country Greece 
Sector Academic/University 
PI Contribution Supervision of laboratory work done
Collaborator Contribution Provision of samples and payment for consumables
Impact None yet. Multidisciplinary.
Start Year 2015
 
Description G/R, Full4 Health 
Organisation University of Aberdeen
Country United Kingdom 
Sector Academic/University 
PI Contribution Supervision of laboratory work done
Collaborator Contribution Provision of samples and payment for consumables
Impact None yet. Multidisciplinary.
Start Year 2015
 
Description G/R, Full4 Health 
Organisation Utrecht University
Country Netherlands 
Sector Academic/University 
PI Contribution Supervision of laboratory work done
Collaborator Contribution Provision of samples and payment for consumables
Impact None yet. Multidisciplinary.
Start Year 2015
 
Description G/R, Holst perfused intestine and pancreas 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Experimental ideas and data
Collaborator Contribution Experiments
Impact Publications, PMIDs: 26280129, 24525020, 26483393
Start Year 2014
 
Description G/R, Karolina Skibicka, central GLP-1 
Organisation University of Gothenburg
Country Sweden 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publication: PMID: 25793511
Start Year 2013
 
Description G/R, Kristina Schoonjans, GPBAR1 
Organisation University of Lausanne
Country Switzerland 
Sector Academic/University 
PI Contribution Experiments and ideas
Collaborator Contribution GM mouse model
Impact Publication, PMID: 26280129
Start Year 2015
 
Description G/R, Medimmune proteomics 
Organisation MedImmune
Country United States 
Sector Private 
PI Contribution We will develop mass spec gut hormone detection
Collaborator Contribution Financial and intellectual
Impact none as yet
Start Year 2015
 
Description G/R, Medimmune studentship 
Organisation MedImmune
Country United States 
Sector Private 
PI Contribution joint supervision of PhD student
Collaborator Contribution joint supervision of PhD student
Impact too early in the studentship
Start Year 2014
 
Description G/R, Novartis RNAseq 
Organisation Novartis
Country Global 
Sector Private 
PI Contribution Providing information, developing new techniques
Collaborator Contribution Development of new techniques
Impact Developed a priliminary technique to isolate RNA from human enteroednocrine cells
Start Year 2012
 
Description G/R, Patrik Rorsman, islet studies 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publications: PMIDs: 25172946, 24315372, 20519125
Start Year 2009
 
Description G/R, Pedro Herrera, Islet transdifferentiation 
Organisation University of Geneva
Country Switzerland 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experimental contributions
Impact Publication: PMID 25141178
Start Year 2013
 
Description G/R, Peter Spegel, islet studies 
Organisation Lund University
Country Sweden 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publication: PMID: 25731850
Start Year 2015
 
Description G/R, Roche TAAR1 
Organisation F. Hoffmann-La Roche AG
Country Global 
Sector Private 
PI Contribution Experiments
Collaborator Contribution provision of information and research compounds
Impact Publication: PMID 26241122
Start Year 2013
 
Description G/R, Thue Schwartz, enteroendocrine GPCRome 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publications: PMIDs: 25453189, 26483393
Start Year 2010
 
Description G/R, Yvan Gosmain, islet studies 
Organisation University of Geneva
Country Switzerland 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publication: PMID: 26696123
Start Year 2014
 
Description GIP receptor localisation and function 
Organisation University of Toronto
Country Canada 
Sector Academic/University 
PI Contribution We generated a gipr knockin mouse, which has been shipped to Toronto
Collaborator Contribution Analysis of the genetically modified mouse in Toronto
Impact None to date
Start Year 2016
 
Description Gastrectomy clinicians 
Organisation Addenbrooke's Hospital
Country United Kingdom 
Sector Hospitals 
PI Contribution Physiological studies on humans after gastrectomy plus in vitro analysis of tissue samples
Collaborator Contribution Identification of suitable patients
Impact Publications
Start Year 2015
 
Description Generation of organoids from transgenic models 
Organisation Royal Netherlands Academy of Arts and Sciences
Department Hubrecht Institute
Country Netherlands 
Sector Academic/University 
PI Contribution We provided tissue from transgenic mouse models
Collaborator Contribution Collaborators will generate organoids for use in their research programme
Impact None to date
Start Year 2016
 
Description Holst perfused pancreas 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Ideas for experiments
Collaborator Contribution Perfused pancreas to test ideas developed in Cambridge
Impact Publication
Start Year 2015
 
Description Human EEC isolation 
Organisation Novartis
Country Global 
Sector Private 
PI Contribution Development of methodology to purify human L-cells
Collaborator Contribution Method development
Impact Methods still under optimisation
Start Year 2011
 
Description LGC peptidomics 
Organisation LGC Ltd
Country United Kingdom 
Sector Private 
PI Contribution Supporting a BBSRC iCASE studentship
Collaborator Contribution Analysis and training
Impact One publication to date
Start Year 2017
 
Description Larraufie Paris lab 
Organisation French National Institute of Agricultural Research
Country France 
Sector Public 
PI Contribution Experimentation
Collaborator Contribution Experimentation and project planning
Impact Publications
Start Year 2016
 
Description Lilly GIPR 
Organisation Eli Lilly & Company Ltd
Country United Kingdom 
Sector Private 
PI Contribution Research in the field of central GIPR signalling
Collaborator Contribution Tool compounds and unpublished data
Impact None yet
Start Year 2018
 
Description MedImmune PhD studentship GLP1R 
Organisation MedImmune
Country United States 
Sector Private 
PI Contribution Joint PhD student with supervisors at Medimmune generating and characterising GLP1R antibodies
Collaborator Contribution MedImmune will provide training and tools
Impact One publication to date
Start Year 2014
 
Description Medimmune BBSRC iCASE (Galvin) 
Organisation MedImmune
Department MedImmune Cambridge
Country United Kingdom 
Sector Private 
PI Contribution Supervision of PhD student
Collaborator Contribution Joint supervision of student, training, peptide synthesis
Impact None yet
Start Year 2016
 
Description Mercodia assay validation 
Organisation Mercodia AB
PI Contribution Mass spec analysis to validate Mercodia's glucagon assay. Measurement of patient samples using glucagon and glicentin assays to establish the cause of assay cross reactivity
Collaborator Contribution Provision of assays and suggestions for protocol changes to remove cross-reactivity
Impact Mercodia published a white paper outlining the collaboration and how it has impacted on the use of the glucagon assay
Start Year 2018
 
Description Pancreatic islet studies 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of transgenic mouse models, technical and practical assistance with FACS sorting, qRT-PCR analysis
Collaborator Contribution Initiated project and produced samples for analysis
Impact Published manuscripts
Start Year 2010
 
Description Shin Lee, ETH Zurich 
Organisation ETH Zurich
Country Switzerland 
Sector Academic/University 
PI Contribution Provision of transgenic mice
Collaborator Contribution Analysis of transgenic mice
Impact None to date
Start Year 2016
 
Description Takeda collaboration 
Organisation Takeda Cambridge Ltd
Country United Kingdom 
Sector Private 
PI Contribution We have initiated 2 projects that have been pursued in collaboration with Takeda
Collaborator Contribution Takeda have knockout mouse models that have been given to the project, and researchers at Takeda have carried out in vivo mouse phenotyping that has contributed to the final output.
Impact PMIDs: 25028498 22190648
Start Year 2009
 
Description Ulster, transgenic mice 
Organisation Ulster University
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration involving transgenic mouse models generated in Cambridge, and shipped to Ulster
Collaborator Contribution Collaborators will work with the transgenic model
Impact None to date
Start Year 2016
 
Description Yuki Oka 
Organisation California Institute of Technology
Department Division of Biology and Biological Engineering (BBE)
Country United States 
Sector Academic/University 
PI Contribution Provision of mouse models
Collaborator Contribution Research contribution
Impact Nature publication
Start Year 2017
 
Description indole as a signalling molecule from gut microbiota 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution A visiting scientist from the department of physics joined our lab part time for 6 months to investigate the physiological role of indole. We provided training, techniques and resources
Collaborator Contribution Our partner provided the salary of the visiting post-doc, and intellectual support
Impact A manuscript is in press
Start Year 2013
 
Title GIP-Cre 
Description GIP-Cre genetically modified mouse model 
IP Reference  
Protection Protection not required
Year Protection Granted
Licensed No
Impact MTAs in place for sharing with academic collaborators
 
Title GLP1R-Cre 
Description Transgenic mice expressing Cre-recombinase driven by the GLP-1 receptor promoter 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed Yes
Impact None known to date
 
Title GLU-Cre mice 
Description Transgenic mice expressing cre-recombinase driven by the proglucagon promoter 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed Yes
Impact No known impact to date
 
Title GLU-CreERT2 
Description Transgenic mice expressing tamoxifen-inducible cre-recombinase driven by the proglucagon promoter 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed Yes
Impact None known to date
 
Description 13th annual Biomarkers Congress 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact An industrial congress bringing together a variety of industrial technologies for the measurement of biomarkers
Year(s) Of Engagement Activity 2018
 
Description ADA poster 2014 (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Triggered interesting questions
Year(s) Of Engagement Activity 2014
 
Description BBC Radio Cambridgeshire Interview 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact Interview with BBC Radio Cambridgeshire, to discuss gut brain signalling, and the upcoming public lecture at the Science Festival
Year(s) Of Engagement Activity 2017
 
Description Big Biology Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Big Biology Day - engagement activities explaining metabolic research to families
Year(s) Of Engagement Activity 2017
 
Description Cambridge Big Weekend 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact hands on events loosley based on the Units research
Year(s) Of Engagement Activity 2015
 
Description Cambridge Network recruitment event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Richard Kay presented at the Cambridge Network recruitment event: "Proglucagon peptide analysis in post gastrectomy patients - bioanalysis or gut feeling?" to people interested in science jobs in the Cambridge area. (asked to present by Mike Van Der Merwe at LGC)
Year(s) Of Engagement Activity 2018
 
Description Cambridge Science Festival - Right Diet debate 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Audience reported change in views, opinions or behaviours
Year(s) Of Engagement Activity 2015
URL http://www.sciencefestival.cam.ac.uk/events/there-right-diet-me
 
Description Cambridge Science Festival - family activities 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Drop-in hands-on event for families and children. Sparked questions from children and parents
Year(s) Of Engagement Activity 2016,2017,2018
 
Description Cambridge Science Festival 2014 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Hands on activity for children sparked discussions with children and parents

Very positive feedback from public
Year(s) Of Engagement Activity 2014
 
Description Cambridge Science Festival Public Lecture 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Public Lecture: Gribble and Fletcher. "Inside out: how the gut changes the brain's view of the world". Followed by questions from the public
Year(s) Of Engagement Activity 2017
 
Description Cambridge Science festival, family weekends 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact promoted interest in science taking place at the inst
Year(s) Of Engagement Activity 2013,2014,2015
 
Description Careers day at Presdales Secondary School (Cheryl Brighton) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Speed-dating job careers advise sparked interesting and stimulating discussions.

Some pupils expressed interest in a career in Science - to early to judge longterm impact.
Year(s) Of Engagement Activity 2014
 
Description Diabetes UK Aug 2014 (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Participants in your research and patient groups
Results and Impact Discussed new treatments for diabetes and their benefits and limitations

Patients expressed interest that we are performing research that might improve future therapies
Year(s) Of Engagement Activity 2014
 
Description Diabetes UK July 2014 Huntingdon group (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Participants in your research and patient groups
Results and Impact Many questions answered about diabetes treatments

Patients better informed about diabetes treatments
Year(s) Of Engagement Activity 2014
 
Description EASD poster 2013 (AEA) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Interesting discussions around data presented on poster

Interesting discussion and future collaborations discussed
Year(s) Of Engagement Activity 2013
 
Description EASD poster 2013 (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Triggered interesting discussion
Year(s) Of Engagement Activity 2013
 
Description EASD poster 2014 (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Triggered interesting discussion and collaboration

Triggered interesting discussion
Year(s) Of Engagement Activity 2014
 
Description FG, BBC Horizon Special - Right Diet for You 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact 3-part BBC2 horizon series
Year(s) Of Engagement Activity 2015
 
Description FG, BBC Radio 4 programme, Inside Health 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact 'Discussion of publication: "Diagnosis of gestational diabetes: falling through the net"
Year(s) Of Engagement Activity 2015
 
Description FG, Press release, article covered by BBC News and multiple international medical publications 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact 'Discussion of publication: "Diagnosis of gestational diabetes: falling through the net"
Year(s) Of Engagement Activity 2015
 
Description Familial Gastric Cancer Study Day (Geoff Roberts) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Patients, carers and/or patient groups
Results and Impact 80-100 participants and family members, with familial gastric cancer, attended a study day to learn more about current research and practice. Geoff Roberts was one of the invited speakers. A number of patients expressed an interest in participating in our future research
Year(s) Of Engagement Activity 2016
 
Description Invited seminar at Genomics Institute of Novartis Research Foundation (FMG) 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Discussion about Novartis' drug development program

The researchers at Novartis used my expertise to help in their ongoing drug development program targeting intestinal enteroendocrine cells
Year(s) Of Engagement Activity 2014
 
Description Invited seminar at Technische Universität München (FMG) 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Sparked discussion and potential collaboration
Year(s) Of Engagement Activity 2013
 
Description Invited seminar at UCL (FMG) 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Triggered discussion and further experiments that we tried together

Further joint experiments following discussions on the day
Year(s) Of Engagement Activity 2014
 
Description Invited talk at Paris Descartes (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact The speaker, P.Richards, was offered a research position on the basis of this talk

Career progression for P.Richards
Year(s) Of Engagement Activity 2014
 
Description MRC Centenary Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact A hands-on practical demonstration, devised and manned by researchers from our group. The practical was based on sweet taste perception and targeted at members of the public. Engaged participation and interesting discussions with the public

Well received from feedback
Year(s) Of Engagement Activity 2013
 
Description MRL Science Slam 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The presenters (a post doc and student together) won a prize for their presentation
Year(s) Of Engagement Activity 2018
 
Description Oxfordshire Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Audience reported change in views, opinions or behaviours
Year(s) Of Engagement Activity 2015
URL http://www.sciencefestival.cam.ac.uk/events/there-right-diet-me
 
Description Professional PhD Networking forum AgroParistech and ABIES doctoral school Doc'Avenir 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Pierre Larraufie: Professional PhD Networking forum AgroParistech and ABIES doctoral school Doc'Avenir, 2017, Round table invited speaker about positions and career after a PhD.
Year(s) Of Engagement Activity 2017
 
Description School visit (CM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact A practical demonstration and associated discussion of the gut, presented by 2 of our PhD students to pupils at a local school.

Good feedback
Year(s) Of Engagement Activity 2013
 
Description Science lesson at primary school (CLM) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Triggered interesting discussions

Greater interest in science and medicine
Year(s) Of Engagement Activity 2012
 
Description Seminar at University of Sheffield (FMG) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Interesting discussion and plans for future collaboration

The Sheffield group have employed one of our previous summer students to do a PhD, and we plan to work together on gut hormone effects on intestinal afferent nerves
Year(s) Of Engagement Activity 2014
 
Description Stimulating innovation, experiences working with industry (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Stimulated discussion
Year(s) Of Engagement Activity 2013
 
Description Summer school 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Alison Douglas Summer School, for ~30 early career researchers from around Europe. I led an interactive morning session on gut hormones.

Very positive feedback received (scored as best presentation from feedback forms)
Year(s) Of Engagement Activity 2013
 
Description Tutor on the Drug Metabolism and Discussion Group (DMDG) large molecule bioanalysis course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Richard Kay was Tutor on the Drug Metabolism and Discussion Group (DMDG) large molecule bioanalysis course. A three day course held at Loughborough University, focussed on the dissemination of knowledge for developing bioanalytical methodologies for large molecule analytes.
Year(s) Of Engagement Activity 2017
 
Description WT PhD Studentship meeting (London 2013) (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Triggered discussion
Year(s) Of Engagement Activity 2013