The Enteroendocrine System in Health and Disease

Lead Research Organisation: University of Cambridge

Abstract

The aims of our research are to understand how hormones from the gut influence appetite and blood glucose control.
After a meal, the gut produces hormones signalling to the brain to stop eating, and to the pancreas that it is time to release more insulin. One of these gut hormones has recently been developed as a new treatment for diabetes, and many people are interested in whether any other gut hormones could also be developed for the treatment of diabetes and obesity.
Our programme of research aims to understand how gut hormones are released after a meal, and whether it would be possible to alter their production as a new form of treatment. We study how individual hormone-producing cells detect nutrients, using techniques that allow us to monitor how single cells respond to stimuli in real time. By removing specific genes in mice, we can build up a picture of which genes are most important for how these gut cells work. The findings can then be used as a starting point for devising new ways to alter hormone release in humans.

Technical Summary

This programme addresses enteroendocrine cell physiology, identifying strategies to enhance secretion of gut hormones that regulate appetite and insulin release.
The gut endocrine system is increasingly recognised as a central player in the coordination of appetite, insulin release and post-prandial nutrient homeostasis. It likely underlies many of the beneficial physiological responses to gastric bypass surgery, and some of the secreted hormones have already been harnessed successfully for the treatment of diabetes and short bowel syndrome. Emerging therapies based on the secretion and action of gut peptides are predicted to be major players in the future treatment of metabolic disease.
Understanding the physiology of the gut endocrine system is critical for identifying novel strategies to modulate hormone release in humans and interpreting post-ingestive and post-bypass physiology. This is an area of research that has advanced dramatically in the past decade, with the introduction of technologies to identify, isolate and interrogate enteroendocrine cells in murine models. As a result, some general principles of stimulus detection in incretin-secreting K- and L-cells have now been established, but there are many unanswered questions such as the relative importance of different enteroendocrine signalling pathways in vivo, the functional overlap between different enteroendocrine cell types, and the impact of genetics, diet and metabolic disease on the enteroendocrine system?
These questions will be addressed here using a candidate gene approach. Genes for functional evaluation will be selected based on their predicted effects on whole body physiology, enteroendocrine cell development, nutrient sensing and/or secretion. State-of-the-art optical and electrophysiological recording techniques will be utilised to monitor dynamic physiological responses of single identified enteroendocrine cells in primary culture, and the intact gut-brain-pancreatic axis will be assessed in vivo using genetically modified mouse models. Promising enteroendocrine target receptors and pathways will be further evaluated for translational effects on appetite and glucose homeostasis in humans.

Organisations

Publications

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Anesten F (2016) Preproglucagon neurons in the hindbrain have IL-6 receptor-a and show Ca2+ influx in response to IL-6. in American journal of physiology. Regulatory, integrative and comparative physiology

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Arora T (2018) Microbial regulation of the L cell transcriptome. in Scientific reports

 
Description JDRF UK Beta Cell Renewal Roundtable
Geographic Reach National 
Policy Influence Type Participation in advisory committee
 
Description MRC Workshop on Neuroscience of Obesity: Gut-brain communication
Geographic Reach National 
Policy Influence Type Participation in advisory committee
 
Description Addenbrooke's Charitable Trust Fellowship (Geoff Roberts)
Amount £52,000 (GBP)
Organisation Addenbrooke's Charitable Trust (ACT) 
Sector Charity/Non Profit
Country United Kingdom
Start 09/2016 
End 09/2017
 
Description BBSRC DTP PhD studentship (Ming Yang)
Amount £95,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2015 
End 09/2019
 
Description BBSRC FTMA to Juraj Rievaj
Amount £14,656 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2018 
End 07/2018
 
Description BBSRC iCASE
Amount £145,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2018 
End 09/2022
 
Description BBSRC iCASE
Amount £105,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2018 
End 09/2022
 
Description BBSRC iCASE studentship partnered with MedImmune (Sam Galvin)
Amount £95,196 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2016 
End 09/2020
 
Description Bakala Foundation Scholarship (Cristian Riccio)
Amount £15,000 (GBP)
Organisation Zdenek and Michaela Bakala foundation 
Sector Charity/Non Profit
Country Switzerland
Start 07/2014 
End 07/2015
 
Description British Society for Neuroendocrinology small grant (Juraj Rievaj)
Amount £3,500 (GBP)
Organisation British Society for Neuroendocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2017 
End 06/2017
 
Description Covid plasma LC-MS analysis
Amount £4,875 (GBP)
Organisation Addenbrooke's Charitable Trust (ACT) 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2020 
End 07/2020
 
Description DAAD-PROMOS-Stipend for Ms Friederike Uhlig
Amount € 1,725 (EUR)
Organisation University of Hohenheim 
Sector Academic/University
Country Germany
Start 08/2013 
End 12/2013
 
Description EASD travel grant
Amount € 300 (EUR)
Organisation European Association for the Study of Diabetes (EASD) 
Sector Charity/Non Profit
Country Germany
Start 09/2018 
End 10/2018
 
Description EFSD
Amount € 122,000 (EUR)
Funding ID rg89241 
Organisation European Association for the Study of Diabetes (EASD) 
Department European Foundation for the Study of Diabetes (EFSD)
Sector Academic/University
Country Germany
Start 05/2017 
End 11/2018
 
Description EFSD Novo Nordisk Foundation future leaders award to Alice Adriaenssens
Amount 500,000 kr. (DKK)
Organisation Novo Nordisk Foundation 
Sector Charity/Non Profit
Country Denmark
Start 01/2022 
End 12/2027
 
Description EFSD Novo Nordisk programme
Amount € 100,000 (EUR)
Organisation European Association for the Study of Diabetes (EASD) 
Department European Foundation for the Study of Diabetes (EFSD)
Sector Academic/University
Country Germany
Start 03/2022 
End 02/2023
 
Description EFSD/Boehringer Ingelheim Programme
Amount € 100,000 (EUR)
Organisation European Association for the Study of Diabetes (EASD) 
Department European Foundation for the Study of Diabetes (EFSD)
Sector Academic/University
Country Germany
Start 07/2014 
End 07/2016
 
Description EMBO Short Term Fellowship (Catalin Chimerel)
Amount € 7,500 (EUR)
Organisation European Molecular Biology Organisation 
Sector Charity/Non Profit
Country Germany
Start 08/2016 
End 12/2016
 
Description ESE St Odile meeting grant
Amount € 450 (EUR)
Organisation European Society of Endocrinology 
Sector Learned Society
Country European Union (EU)
Start 09/2018 
End 10/2018
 
Description Early career grant to Juraj Rievaj
Amount £8,588 (GBP)
Organisation Society for Endocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2017 
End 06/2018
 
Description Early career grant to Van Lu
Amount £10,000 (GBP)
Organisation Society for Endocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2017 
End 07/2018
 
Description G/R, Joint Wellcome Trust Senior Investigators
Amount £1,900,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 06/2015 
End 06/2020
 
Description GIPR in the CNS
Amount $260,000 (USD)
Organisation Eli Lilly & Company Ltd 
Department Neuroscience Eli Lilly
Sector Private
Country United States
Start 01/2021 
End 12/2021
 
Description Jo Lewis, BSN symposium support for Physiology 2019, Aberdeen, UK
Amount £2,000 (GBP)
Organisation British Society for Neuroendocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2019 
End 01/2019
 
Description Jo Lewis, BSN symposium support for the ECR day at the BSN's annual conference, Glasgow, UK
Amount £5,000 (GBP)
Organisation British Society for Neuroendocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2019 
End 01/2019
 
Description Jo Lewis, The Physiological Society satellite support for Physiology 2019, Aberdeen, UK
Amount £20,000 (GBP)
Organisation Physiological Society 
Sector Charity/Non Profit
Country Global
Start 07/2019 
End 08/2019
 
Description Jo Lewis, The Physiological Society symposium support for Experimental Biology, Orlando,
Amount £2,000 (GBP)
Organisation Physiological Society 
Sector Charity/Non Profit
Country Global
Start 05/2019 
End 06/2019
 
Description Lister Summer Studentship
Amount £1,600 (GBP)
Organisation Lister Institute of Preventive Medicine 
Sector Charity/Non Profit
Country United Kingdom
Start 06/2013 
End 08/2013
 
Description Liver disease peptidomics and proteomics
Amount £24,350 (GBP)
Organisation National Institute for Health Research 
Department NIHR Biomedical Research Centre
Sector Public
Country United Kingdom
Start 02/2020 
End 12/2020
 
Description MedImmune PhD studentship programme
Amount £96,074 (GBP)
Organisation AstraZeneca 
Department MedImmune
Sector Private
Country United Kingdom
Start 09/2014 
End 09/2018
 
Description Medimmune grant
Amount £272,857 (GBP)
Organisation AstraZeneca 
Department MedImmune
Sector Private
Country United Kingdom
Start 05/2015 
End 08/2017
 
Description Peptidomics of gestational diabetes
Amount £3,400 (GBP)
Organisation University of Cambridge 
Sector Academic/University
Country United Kingdom
Start 05/2020 
End 03/2021
 
Description PhD in Clinical Biochemistry
Amount £100,000 (GBP)
Funding ID 2430172 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 08/2020 
End 03/2024
 
Description Project Support Grant to Juraj Rievaj
Amount £3,680 (GBP)
Organisation British Society for Neuroendocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2017 
End 03/2018
 
Description Royal College of Surgeons Research Fellowship
Amount £20,000 (GBP)
Organisation Royal College of Surgeons of England 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2017 
End 09/2018
 
Description Society for Endocrinology Early Career Grant (Arianna Psichas)
Amount £10,000 (GBP)
Organisation Society for Endocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2015 
End 03/2016
 
Description Society for Endocrinology Early Career Grant (Catalin Chimerel)
Amount £9,924 (GBP)
Organisation Society for Endocrinology 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2017 
End 05/2017
 
Description Targeting the gut in metabolic disease
Amount £2,381,203 (GBP)
Funding ID 220271/Z/20/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2020 
End 07/2025
 
Description Wellcome PhD studentship to Elisabeth O'Flaherty
Amount £180,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 09/2019 
End 09/2022
 
Description Wellcome PhD studentship to Emily Miedzybrodzka
Amount £180,824 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 09/2018 
End 09/2021
 
Title G/R, Measurement of GLP-1 from epithelial sheets 
Description Optimising the use of Ussing chambers technology for the reliable measurement of basolateral GLP-1 secretion from epithelial sheets 
Type Of Material Technology assay or reagent 
Year Produced 2015 
Provided To Others? Yes  
Impact Method allowed to distinguish between apical and basolateral effect of various drugs on GLP-1 secretion (Brighton et al, 2015) 
URL http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606749/
 
Title GIP-Venus transgenic mouse 
Description Venus driven by the GIP promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2009 
Provided To Others? Yes  
Impact Shared with some other groups and licensed 
 
Title GLU-Cre transgenic mouse 
Description Cre-recombinase driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2012 
Provided To Others? Yes  
Impact Shared with other groups and licensed to industry 
 
Title GLU-Venus transgenic mouse 
Description Fluorescent protein driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2008 
Provided To Others? Yes  
Impact Shared with many collaborators around the world and licensed to industry 
 
Title Gipr-Cre transgenic mouse 
Description GIPR-Cre mouse enabling identification and manipulation of cells expressing Gipr 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2016 
Provided To Others? Yes  
Impact Collaborations and publications. Research collaboration with Eli Lilly, investigating MOA of GLP1/GIP dual agonists (currently in phase 3 clinical trials) 
 
Title Glu-CreERT2 
Description Tamoxifen-inducible cre expression driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2017 
Provided To Others? Yes  
Impact Shared with academic collaborators and licensed to industry 
 
Title Glu-Epac2camps transgenic mouse 
Description transgenic mice in which the cAMP FRET sensor Epac2camps is driven by the proglucagon promoter 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2016 
Provided To Others? Yes  
Impact Shared with collaborators 
 
Title Guanylin-Venus transgenic mouse 
Description Guanylin-venus mouse, enabling identification and characterisation of cells expressing guanylin 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2019 
Provided To Others? Yes  
Impact Publication 
 
Title Insl5-rtTA mouse 
Description transgenic mouse Insl5-rtTA allowing identification and manipulation of INSL5-expressing cells 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2018 
Provided To Others? Yes  
Impact publications 
 
Title Rxfp4-Cre transgenic mouse 
Description Rxfp4-Cre transgenic mouse 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2015 
Provided To Others? No  
Impact BBSRC-iCASE studentship joint with AstraZeneca to investigate central signalling involving Rxfp4 
 
Title human GLU-Venus 
Description Human ileal organoids expressing the fluorescent reporter Venus driven by the proglucagon promoter 
Type Of Material Cell line 
Year Produced 2020 
Provided To Others? Yes  
Impact requests for collaborations 
 
Title sst-cre transgenic mouse 
Description Cre-recombinase driven by the somatostatin promoter in a transgenic mouse model 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2015 
Provided To Others? Yes  
Impact Shared with academic collaborators 
 
Title Alteration of the plasma peptidome of gastrectomised patients during an OGTT 
Description Performing gastric bypass surgery on patients severely changes their gut hormone response to glucose. The plasma concentrations of Insulin and a number of associated incretin peptides are significantly raised shortly after ingestion of glucose. Typically the plasma concentrations of these peptides are monitored using immunoassays. A simple and high throughput plasma peptidomics approach was used to simulatneously monitor a large number of these peptides. Comparison of gastresctomised and control individuals showed significant increases of insulin, glucagon gene products, PYY and neurotensin. 
Type Of Material Database/Collection of data 
Year Produced 2020 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ebi.ac.uk/pride/archive/projects/PXD016690
 
Title Characterization of Guca2a expressing cells in the mouse intestine 
Description RNA seq of Guca2a expressing cells 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact openly accessible data 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE128117
 
Title Co-storage and release of insulin-like peptide-5, glucagon-like peptide-1 and peptideYY from murine and human colonic enteroendocrine cells 
Description Analysis of vesicular colocalisation by 3D-SIM 
Type Of Material Data analysis technique 
Year Produced 2018 
Provided To Others? Yes  
Impact open method 
 
Title Expected values for gastrointestinal and pancreatic hormone concentrations in healthy volunteers in the fasting and postprandial state 
Description Background: Gastrointestinal hormones regulate intestinal transit, control digestion, influence appetite and promote satiety. Altered production or action of gut hormones, including glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP) and peptide YY (PYY), may contribute to the biological basis of obesity and altered glucose homeostasis. However, challenges in analytical methodology and lack of clarity on expected values for healthy individuals has limited progress in this field. The aim of this study was to describe expected concentrations of gastrointestinal and pancreatic hormones in healthy volunteers following a standardised meal test (SMT) or 75g oral glucose tolerance test (OGTT). Methods: 28 healthy volunteers (12 men, 16 women; mean age 31.3 years; mean body mass index 24.9 kg/m2) were recruited to attend a hospital clinic on two occasions. Volunteers had blood sampling in the fasting state and were given, in randomised order, an oral glucose tolerance test (OGTT) and standardised mixed liquid meal test with venepuncture at timed intervals for 4 hours after ingestion. Analytical methods for gut and pancreatic hormones were assessed and optimised. Concentrations of gut and pancreatic hormones were measured and used to compile ranges of expected values. Results: Ranges of expected values were created for glucose, insulin, glucagon, GLP-1, GIP, PYY and free fatty acids in response to a standardised mixed liquid meal or OGTT. Intact Proinsulin and C-peptide levels were also measured following the OGTT. Conclusions: These ranges of expected values can now be used to compare gut hormone concentrations between healthy individuals and patient groups. 
Type Of Material Database/Collection of data 
Year Produced 2020 
Provided To Others? Yes  
Impact Openly accessible dataset 
URL https://www.repository.cam.ac.uk/handle/1810/312528
 
Title Glucose-dependent Insulinotropic polypeptide receptor-expressing cells in the hypothalamus regulate food intake 
Description Ambiguity regarding the role of glucose-dependent insulinotropic polypeptide (GIP) in obesity arises from conflicting reports asserting that both GIP receptor (GIPR) agonism and antagonism are effective strategies for inhibiting weight gain. To enable identification and manipulation of Gipr-expressing (Gipr) cells we created GIPR-Cre knock-in mice. As GIPR-agonists have recently been reported to suppress food intake we aimed to identify central mediators of this effect. Gipr cells were identified in the arcuate, dorsomedial, and paraventricular nuclei of the hypothalamus, as confirmed by RNAscope in mouse and human. Single cell RNAseq identified clusters of hypothalamic Gipr cells exhibiting transcriptomic signatures for mural, glial and neuronal cells, the latter expressing somatostatin, but little proopiomelanocortin or agouti-related peptide. Activation of Gq-DREADDs in hypothalamic Gipr cells suppressed food intake in vivo, which was not obviously additive with concomitant GLP1R activation. These data identify hypothalamic GIPR as a target for the regulation of energy balance. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact openly accessible dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE134726
 
Title Human enteroendocrine cell transcriptomic profiling 
Description We purified two populations of human jejunal enteroendocrine cells (GLP1+ and GLP1-) by FACS and identified transcripts enriched in endocrine cell lineages. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact open accessible dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE114853
 
Title Human intestinal organoid cell transcriptomic profiling 
Description We purified two populations of human ileal enteroendocrine cells (Venus+ and Venus-) from mature differentiated hGLU-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages. 
Type Of Material Database/Collection of data 
Year Produced 2020 
Provided To Others? Yes  
Impact openly accessible dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE148224
 
Title Mouse duodenal enteroendocrine transcriptome 
Description Using genetically modifed mice to express fluorescent reporter in proglucagon expressing cells (GluVenus) or in all enteroendocrine cells (NeuroD1), we purifed positive and negative cells by FACS from duodenal epithelial cells and identified transcripts enriched in positive populations 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact open accessible dateset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE114913
 
Title Peptidomic analysis of pancreatic neurodendocrine tumours (Glucagonoma) 
Description A peptidomic analysis of plasma from patients with well characterised pancreatic neuroendocrine tumours (PNET) was performed. A nano LC-MS analysis identified a number of peptides from the glucagon gene, which were idenfiried in a previous case study using multiple immunoassays. Peptides to other proteins involved in peptide secretion were also identified. Plasma from a second glucagonoma patient was analysed using a high flow approach, and this identified similar peptides to the nano analysis. In order to demonstrate the potential clinical application of LC-MS to characterising neuroendocrine tumours, a large cohort of plasma samples were analysed to demonstrate the ability of this approach to identify glucagonoma patients, and differentiate from a single insulinoma patient 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ebi.ac.uk/pride/archive/projects/PXD008465
 
Title Peptidomics of sorted enteroendocrine cells from human organoids 
Description A human organoid culture system was set up to grow enteroendocrine cells with a venus labeled on the glucagon gene promoter sequence. This enabled the sorting of glucagon gene positive cells from negative cells, thereby enabling the enrichment of glucagon producing cells for study. Both Venus positive and venus negative cell populations were collected and their peptidome was assessed using nano LC-MS/MS 
Type Of Material Database/Collection of data 
Year Produced 2020 
Provided To Others? Yes  
Impact open data 
URL https://www.ebi.ac.uk/pride/archive/projects/PXD017825
 
Title Peptidomics profiling of biopsies from the human jejunum before and after gastrectomy 
Description Profiling of the human jejunum peptidome before and after total gastrectomy. The study compares the peptidome of jejunum using intact mass spectrometry and database searching. These peptides included most gut hormones including proglucagon derived peptides, PYY, GIP, Motilin somatostatin and neurotensin amongst others. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ebi.ac.uk/pride/archive/projects/PXD011498
 
Title Peptidomics profiling of human intestine 
Description The aim of the project is to generate a peptidomics map of gut hormone peptides along the gastrointestinal tract, starting with the stomach and including the duodenum, jejunum, ileum, ascending colon, sigmoid colon and rectum. The tissues would be collected after surgery and the peptide fraction extracted and anlysed by nano LC-MS to identify what peptide hormones are present. These data will then be used to compare against the human transcriptome, and also for comparison against equivent peptides from murine intestinal extracts. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ebi.ac.uk/pride/archive/projects/PXD009788
 
Title Peptidomics profiling of murine intestine 
Description The aim of the project is to generate a peptidomics map of gut hormone peptides along the gastrointestinal tract, starting with the stomach and including the duodenum, jejunum, ileum, colon and rectum. The tissues would be collected after surgery and the peptide fraction extracted and anlysed by nano LC-MS to identify what peptide hormones are present. These data will then be used to compare against the murine transcriptome, and also for comparison against equivent peptides from human intestinal extracts. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ebi.ac.uk/pride/archive/projects/PXD009796
 
Title Peptidomics profiling of the murine gut after vertical sleeve gastrectomy 
Description Profiling of the murine peptidome along the gastrointestinal tract and pancreas in vertical sleeve gastrectomy and sham operated mice. The study compares the peptidome of various portions of the GI tract using intact mass spectrometry and database searching. These peptides included most gut hormones including proglucagon derived peptides, PYY, GIP, somatostatin, neurotensin amongst others. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ebi.ac.uk/pride/archive/projects/PXD011455
 
Title SCFAs strongly stimulate PYY production in human enteroendocrine cells. 
Description Peptide-YY (PYY) and Glucagon-Like Peptide-1 (GLP-1) play important roles in the regulation of food intake and insulin secretion, and are of translational interest in the field of obesity and diabetes. PYY production is highest in enteroendocrine cells located in the distal intestine, mirroring the sites where high concentrations of short chain fatty acids (SCFAs) are produced by gut microbiota. We show here that propionate and butyrate strongly increased expression of PYY but not GCG in human cell line and intestinal primary culture models. The effect was predominantly attributable to the histone deacetylase inhibitory activity of SCFA and minor, but significant contributions of FFA2 (GPR43). Consistent with the SCFA-dependent elevation of PYY gene expression, we also observed increased basal and stimulated PYY hormone secretion. Interestingly, the transcriptional stimulation of PYY was specific to human-derived cell models and not reproduced in murine primary cultures. This is likely due to substantial differences in PYY gene structure between mouse and human. In summary, this study revealed a strong regulation of PYY production by SCFA that was evident in humans but not mice, and suggests that high fibre diets elevate plasma concentrations of the anorexigenic hormone PYY, both by targeting gene expression and hormone secretion. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact Openly accessible data 
URL https://www.repository.cam.ac.uk/handle/1810/273309
 
Title Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice 
Description We report the different enteroendocrine populations in the large intestine in mice based on their transcriptomics profile. Hormone, receptor and transcription factors are analysed for differential expression between the different cell clusters to give a comprehensive view of the different characterisitcs of the distal entereondocrine cells 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact openly accessible dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE137572
 
Title Single-cell RNA-sequencing reveals distinct populations of glucagon-like peptide-1 producing cells in the mouse upper small intestine 
Description Enteroendocrine L-cells release hormones that control metabolism and appetite and are targets under investigation for the treatment of diabetes and obesity. Understanding L-cell diversity and expression profiles is critical for identifying target receptors that will translate into altered hormone secretion. We performed single cell RNA sequencing of mouse L-cells from the upper small intestine to distinguish cellular populations, revealing that L-cells form 3 major clusters: a group with typical characteristics of classical L-cells, including high expression of Gcg and Pyy; a cell type overlapping with Gip-expressing K-cells; and a unique cluster expressing Tph1 and Pzp that was predominantly located in duodenal villi and co-produced 5HT. Expression of G-protein coupled receptors differed between clusters, suggesting the cell types are differentially regulated, and would be differentially targetable. Our findings support the emerging concept that many enteroendocrine cell populations are highly overlapping, with individual cells producing a range of peptides previously assigned to distinct cell types. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE108108
 
Title The SNARE Protein, Syntaxin1a, Plays an Essential Role in Biphasic Exocytosis of the Incretin Hormone, Glucagon-Like Peptide-1 
Description Exocytosis of the hormone, glucagon-like peptide-1 (GLP-1), by the intestinal L-cell is essential for the incretin effect after nutrient ingestion, and is critical for the actions of dipeptidylpeptidase IV inhibitors that enhance GLP-1 levels in patients with type 2 diabetes. 2-Photon microscopy revealed that exocytosis of GLP-1 is biphasic, with a 1(st) peak at 1-6min and a 2(nd) peak at 7-12min after stimulation with forskolin. Approximately 75% of the exocytotic events were represented by compound granule fusion, and the remainder were accounted for by full fusion of single granules, under basal and stimulated conditions. The core SNARE protein, syntaxin-1a (syn1a), was expressed by murine ileal L-cells. At the single L-cell level, 1(st) phase forskolin-induced exocytosis was reduced to basal (p 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact openly accessible data 
URL https://www.repository.cam.ac.uk/handle/1810/265784
 
Title The effect on the transcriptome by inhibition of mitochondrial function by metformin in intestinal cells 
Description We purified mouse duodenal cells from organoids derived from two different transgenic mouse lines (GIP-Cre tdRFP, SST-Cre tdRFP), and identified transcripts enriched in metformin-treated cells. 
Type Of Material Database/Collection of data 
Year Produced 2021 
Provided To Others? Yes  
Impact openly accessible dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE165122
 
Title Transcriptomic profiling of alpha, beta, and delta cell populations provides new insights into the role of ghrelin in the pancreas 
Description Intra-islet crosstalk between islet cells is critical in orchestrating the body's response to changes in blood glucose levels, but is incompletely understood. In this study, we used transgenic mouse lines that allowed the purification and transcriptomic characterisation of alpha, beta, and delta cells, yielding an RNA-sequencing database that can be searched for regulatory proteins which are differentially expressed between cell types. As an illustrative example, we examined the expression of g-protein coupled receptors, and found that the ghrelin receptor, Ghsr, was highly expressed in delta cells compared to alpha and beta cells. GHSR excitation elicited increases in cytosolic calcium levels in primary delta cells. In the perfused pancreas, the application of ghrelin stimulated somatostatin secretion, correlating with a decrease in insulin and glucagon release, which was sensitive to somatostatin receptor antagonism. These results show that ghrelin acts specifically on delta cells within pancreatic islets to affect blood glucose regulation. 
Type Of Material Database/Collection of data 
Year Produced 2016 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE76017
 
Title Transcriptomics analysis of enteroendocrine cells after vertical sleeve gastrectomy 
Description We report here the transcriptome of sorted enteroendocrine cells from duodenum, ileum and colon from mice after vertical sleeve gastrectomy or sham operation, weight matched or not. We analyze the effect of region of origin and surgery and gene expression and found that only region of origin had an impact on EEC transcriptome 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE121489
 
Title Transcriptomics analysis of human jejunal enteroendocrine cells after vertical sleeve gastrectomy 
Description We report here the transcriptome of a population enriched in enteroendocrine cells from human jejunum from patients before or after gastrectomy. We analyze the effect of surgery on gene expression and found very little effect on EEC transcriptome 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact open dataset 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE121486
 
Description Acosta 
Organisation Mayo Clinic
Country United States 
Sector Charity/Non Profit 
PI Contribution Training provided to Acosta's group
Collaborator Contribution Analysis of patient samples
Impact None
Start Year 2018
 
Description Ana Garcia nanoparticles 
Organisation Catholic University of Louvain
Country Belgium 
Sector Academic/University 
PI Contribution We are hosting a post doc in our group
Collaborator Contribution Our partners are funding the project and provided the initial study design
Impact Extension of the partnership from 1 year to 2 years
Start Year 2020
 
Description Brubaker lab 
Organisation University of Toronto
Country Canada 
Sector Academic/University 
PI Contribution Mouse models and discussion
Collaborator Contribution Experimentation and project design
Impact Publication
Start Year 2015
 
Description Central GLP1 signalling 
Organisation University College London
Department Biosciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Longstanding collaboration with Stefan Trapp's laboratory. We currently (Mar 21) have a joint application for further funding from MRC pending, which would financially contribute to the Reimann/Gribble laboratories. We frequently exchange ideas, have provided critical mouse models for this research and transcriptomic capabilities.
Collaborator Contribution see above
Impact Prof. Trapp's and the Reimann/Gribble laboratory have so far co-published 18 research papers on the topic (as on 10Mar21)
 
Description FG, Susan Jebb, HNR 
Organisation Medical Research Council (MRC)
Department MRC Human Nutrition Research Group
Country United Kingdom 
Sector Academic/University 
PI Contribution Assays and discussion
Collaborator Contribution Ran project
Impact Publication, PMID: 26054049
Start Year 2014
 
Description Full4Health 
Organisation Danone
Country United Kingdom 
Sector Private 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Encap Drug Delivery
Country United Kingdom 
Sector Private 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Harokopio University
Country Greece 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Maastricht University (UM)
Country Netherlands 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Norwegian University of Science and Technology (NTNU)
Country Norway 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University Medical Center Utrecht (UMC)
Country Netherlands 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Aberdeen
Country United Kingdom 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Cologne
Country Germany 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Gothenburg
Country Sweden 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Leeds
Country United Kingdom 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation University of Lille
Country France 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description Full4Health 
Organisation Wageningen University & Research
Country Netherlands 
Sector Academic/University 
PI Contribution We run 2 workpackages in this consortium. One is a basic science programme and the other a translational programme.
Collaborator Contribution The overall consortium brings funding to our lab, and Encap are manufacturing clinical grade capsules for our translational study. The University of Copenhagen have sent post-graduate students to our lab for a few months to establish collaborations and learn new techniques. Blood samples collected by other partners will be analysed here in Cambridge for metabolic parameters and gut hormones.
Impact Meetings Summer school Publications Collaborations
Start Year 2011
 
Description G/R Bertie Goettgens, Single Cell RNAseq 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Experiments
Collaborator Contribution Experiments
Impact none yet
Start Year 2015
 
Description G/R, Bart Staels, FXR 
Organisation University of Lille
Country France 
Sector Academic/University 
PI Contribution Experiments and data
Collaborator Contribution Experiments
Impact Publication, PMID: 26134028
Start Year 2014
 
Description G/R, Eelco de Koning, gut organoids 
Organisation Utrecht University
Country Netherlands 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice) Publications: PMIDs: 25500886, 24130334
Collaborator Contribution Experiments
Impact Publications: PMIDs: 25500886, 2413033
Start Year 2013
 
Description G/R, Fredrik Backhed, Germ free/ gut hormones 
Organisation University of Gothenburg
Country Sweden 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Provision of germ free animals / experiments
Impact none as yet
Start Year 2015
 
Description G/R, Full4 Health 
Organisation Harokopio University
Country Greece 
Sector Academic/University 
PI Contribution Supervision of laboratory work done
Collaborator Contribution Provision of samples and payment for consumables
Impact None yet. Multidisciplinary.
Start Year 2015
 
Description G/R, Full4 Health 
Organisation University of Aberdeen
Country United Kingdom 
Sector Academic/University 
PI Contribution Supervision of laboratory work done
Collaborator Contribution Provision of samples and payment for consumables
Impact None yet. Multidisciplinary.
Start Year 2015
 
Description G/R, Full4 Health 
Organisation Utrecht University
Country Netherlands 
Sector Academic/University 
PI Contribution Supervision of laboratory work done
Collaborator Contribution Provision of samples and payment for consumables
Impact None yet. Multidisciplinary.
Start Year 2015
 
Description G/R, Holst perfused intestine and pancreas 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Experimental ideas and data
Collaborator Contribution Experiments
Impact Publications, PMIDs: 26280129, 24525020, 26483393
Start Year 2014
 
Description G/R, Karolina Skibicka, central GLP-1 
Organisation University of Gothenburg
Country Sweden 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publication: PMID: 25793511
Start Year 2013
 
Description G/R, Kristina Schoonjans, GPBAR1 
Organisation University of Lausanne
Country Switzerland 
Sector Academic/University 
PI Contribution Experiments and ideas
Collaborator Contribution GM mouse model
Impact Publication, PMID: 26280129
Start Year 2015
 
Description G/R, Medimmune proteomics 
Organisation AstraZeneca
Department MedImmune
Country United Kingdom 
Sector Private 
PI Contribution We will develop mass spec gut hormone detection
Collaborator Contribution Financial and intellectual
Impact none as yet
Start Year 2015
 
Description G/R, Medimmune studentship 
Organisation AstraZeneca
Department MedImmune
Country United Kingdom 
Sector Private 
PI Contribution joint supervision of PhD student
Collaborator Contribution joint supervision of PhD student
Impact too early in the studentship
Start Year 2014
 
Description G/R, Novartis RNAseq 
Organisation Novartis
Country Global 
Sector Private 
PI Contribution Providing information, developing new techniques
Collaborator Contribution Development of new techniques
Impact Developed a priliminary technique to isolate RNA from human enteroednocrine cells
Start Year 2012
 
Description G/R, Patrik Rorsman, islet studies 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publications: PMIDs: 25172946, 24315372, 20519125
Start Year 2009
 
Description G/R, Pedro Herrera, Islet transdifferentiation 
Organisation University of Geneva
Country Switzerland 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experimental contributions
Impact Publication: PMID 25141178
Start Year 2013
 
Description G/R, Peter Spegel, islet studies 
Organisation Lund University
Country Sweden 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publication: PMID: 25731850
Start Year 2015
 
Description G/R, Roche TAAR1 
Organisation F. Hoffmann-La Roche AG
Country Global 
Sector Private 
PI Contribution Experiments
Collaborator Contribution provision of information and research compounds
Impact Publication: PMID 26241122
Start Year 2013
 
Description G/R, Thue Schwartz, enteroendocrine GPCRome 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publications: PMIDs: 25453189, 26483393
Start Year 2010
 
Description G/R, Yvan Gosmain, islet studies 
Organisation University of Geneva
Country Switzerland 
Sector Academic/University 
PI Contribution Experiments/ Provision of Research Tools (mice)
Collaborator Contribution Experiments
Impact Publication: PMID: 26696123
Start Year 2014
 
Description GIP receptor localisation and function 
Organisation University of Toronto
Country Canada 
Sector Academic/University 
PI Contribution We generated a gipr knockin mouse, which has been shipped to Toronto
Collaborator Contribution Analysis of the genetically modified mouse in Toronto
Impact None to date
Start Year 2016
 
Description Gastrectomy clinicians 
Organisation Addenbrooke's Hospital
Country United Kingdom 
Sector Hospitals 
PI Contribution Physiological studies on humans after gastrectomy plus in vitro analysis of tissue samples
Collaborator Contribution Identification of suitable patients
Impact Publications
Start Year 2015
 
Description Generation of organoids from transgenic models 
Organisation Royal Netherlands Academy of Arts and Sciences
Department Hubrecht Institute
Country Netherlands 
Sector Academic/University 
PI Contribution We provided tissue from transgenic mouse models
Collaborator Contribution Collaborators will generate organoids for use in their research programme
Impact None to date
Start Year 2016
 
Description Heisler. CNS appetite circuitry 
Organisation University of Aberdeen
Country United Kingdom 
Sector Academic/University 
PI Contribution Transgenic mouse models
Collaborator Contribution Data analysis
Impact Publication
Start Year 2019
 
Description Holst perfused pancreas 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Ideas for experiments
Collaborator Contribution Perfused pancreas to test ideas developed in Cambridge
Impact Publication
Start Year 2015
 
Description Human EEC isolation 
Organisation Novartis
Country Global 
Sector Private 
PI Contribution Development of methodology to purify human L-cells
Collaborator Contribution Method development
Impact Methods still under optimisation
Start Year 2011
 
Description LGC peptidomics 
Organisation LGC Ltd
Country Global 
Sector Private 
PI Contribution Supporting a BBSRC iCASE studentship
Collaborator Contribution Analysis and training
Impact One publication to date
Start Year 2017
 
Description Larraufie Paris lab 
Organisation French National Institute of Agricultural Research
Country France 
Sector Academic/University 
PI Contribution Experimentation
Collaborator Contribution Experimentation and project planning
Impact Publications
Start Year 2016
 
Description Lilly GIPR 
Organisation Eli Lilly & Company Ltd
Country United Kingdom 
Sector Private 
PI Contribution Research in the field of central GIPR signalling
Collaborator Contribution Tool compounds and unpublished data
Impact None yet
Start Year 2018
 
Description MedImmune PhD studentship GLP1R 
Organisation AstraZeneca
Department MedImmune
Country United Kingdom 
Sector Private 
PI Contribution Joint PhD student with supervisors at Medimmune generating and characterising GLP1R antibodies
Collaborator Contribution MedImmune will provide training and tools
Impact One publication to date
Start Year 2014
 
Description Medimmune BBSRC iCASE (Galvin) 
Organisation AstraZeneca
Department MedImmune
Country United Kingdom 
Sector Private 
PI Contribution Supervision of PhD student
Collaborator Contribution Joint supervision of student, training, peptide synthesis
Impact None yet
Start Year 2016
 
Description Mercodia assay validation 
Organisation Mercodia AB
Country Sweden 
Sector Private 
PI Contribution Mass spec analysis to validate Mercodia's glucagon assay. Measurement of patient samples using glucagon and glicentin assays to establish the cause of assay cross reactivity
Collaborator Contribution Provision of assays and suggestions for protocol changes to remove cross-reactivity
Impact Mercodia published a white paper outlining the collaboration and how it has impacted on the use of the glucagon assay
Start Year 2018
 
Description Moffett. Trophoblast physiology 
Organisation University of Cambridge
Department Department of Pathology
Country United Kingdom 
Sector Academic/University 
PI Contribution Application of LC-MS/MS peptidomics to analysis of trophoblast organoids
Collaborator Contribution Development of trophoblast organoids
Impact Publication
Start Year 2018
 
Description Pancreatic islet studies 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of transgenic mouse models, technical and practical assistance with FACS sorting, qRT-PCR analysis
Collaborator Contribution Initiated project and produced samples for analysis
Impact Published manuscripts
Start Year 2010
 
Description Rinaman, Fadool, Williams. Central GLP-1 signalling 
Organisation Florida State University
Country United States 
Sector Academic/University 
PI Contribution Provision of transgenic mouse models
Collaborator Contribution Dissection of CNS appetite circuitry
Impact multiple publications
Start Year 2013
 
Description Shin Lee, ETH Zurich 
Organisation ETH Zurich
Country Switzerland 
Sector Academic/University 
PI Contribution Provision of transgenic mice
Collaborator Contribution Analysis of transgenic mice
Impact None to date
Start Year 2016
 
Description Takeda collaboration 
Organisation Takeda Cambridge Ltd
Country United Kingdom 
Sector Private 
PI Contribution We have initiated 2 projects that have been pursued in collaboration with Takeda
Collaborator Contribution Takeda have knockout mouse models that have been given to the project, and researchers at Takeda have carried out in vivo mouse phenotyping that has contributed to the final output.
Impact PMIDs: 25028498 22190648
Start Year 2009
 
Description Ulster, transgenic mice 
Organisation Ulster University
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration involving transgenic mouse models generated in Cambridge, and shipped to Ulster
Collaborator Contribution Collaborators will work with the transgenic model
Impact None to date
Start Year 2016
 
Description Valverde. PTP1B in GLP-1 secretion 
Organisation Autonomous University of Madrid
Country Spain 
Sector Academic/University 
PI Contribution Hosted visiting student and provided training
Collaborator Contribution Visiting student brought project
Impact Publication
Start Year 2017
 
Description Yuki Oka 
Organisation California Institute of Technology
Department Division of Biology and Biological Engineering (BBE)
Country United States 
Sector Academic/University 
PI Contribution Provision of mouse models
Collaborator Contribution Research contribution
Impact Nature publication
Start Year 2017
 
Description indole as a signalling molecule from gut microbiota 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution A visiting scientist from the department of physics joined our lab part time for 6 months to investigate the physiological role of indole. We provided training, techniques and resources
Collaborator Contribution Our partner provided the salary of the visiting post-doc, and intellectual support
Impact A manuscript is in press
Start Year 2013
 
Title GIP-Cre 
Description GIP-Cre genetically modified mouse model 
IP Reference  
Protection Protection not required
Year Protection Granted
Licensed No
Impact MTAs in place for sharing with academic collaborators
 
Title GLP1R-Cre 
Description Transgenic mice expressing Cre-recombinase driven by the GLP-1 receptor promoter 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed Yes
Impact None known to date
 
Title GLU-Cre mice 
Description Transgenic mice expressing cre-recombinase driven by the proglucagon promoter 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed Yes
Impact No known impact to date
 
Title GLU-CreERT2 
Description Transgenic mice expressing tamoxifen-inducible cre-recombinase driven by the proglucagon promoter 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed Yes
Impact None known to date
 
Description 13th annual Biomarkers Congress 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact An industrial congress bringing together a variety of industrial technologies for the measurement of biomarkers
Year(s) Of Engagement Activity 2018
 
Description ADA poster 2014 (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Triggered interesting questions
Year(s) Of Engagement Activity 2014
 
Description AEA ADA talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Alice Adriaenssens ADA 2021: invited talk for 'New turf for the other incretin' Diabetes journal symposium

Follow up = invitation to contribute opinion piece in Diabetes, Feature article in 'ADA Meeting News'
Year(s) Of Engagement Activity 2021
 
Description AEA SSIB talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact SSIB 2021: accepted talk
Follow up = won Harry Kissileff prize for best talk, invitation to contribute to Appetite journal
Year(s) Of Engagement Activity 2021
 
Description AEA. Oral presentation at Society for the Study of Ingestive Behaviour conference; Utrecht, Netherlands. 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Research talk at Society for the Study of Ingestive Behaviour conference; Utrecht, Netherlands.
Year(s) Of Engagement Activity 2019
 
Description AEA. Oral presentation at BSN Neuroendocrinology Celebrate Symposium for ECRs; Glasgow, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited talk at BSN Neuroendocrinology Celebrate Symposium for Early Career Researchers; Glasgow, UK
Year(s) Of Engagement Activity 2019
 
Description AEA. Oral presentation at Experimental Biology conference; Orlando, US. 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited speaker at Experimental Biology conference; Orlando, US. Networking
Year(s) Of Engagement Activity 2019
 
Description AEA. Oral presentation to Eli Lilly; Indianapolis, US, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Research presentation to Eli Lilly.
Year(s) Of Engagement Activity 2019
 
Description AEA. Poster at EASD Incretin Study Group 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact AEA Poster at Incretin Study Group "The role of glucose-dependent insulinotropic polypeptide receptor-expressing neurons in the hypothalamus"
Year(s) Of Engagement Activity 2019
 
Description AEA. Poster at NovoNordisk Foundation Metabolism in Action conference; Copenhagen, Denmark 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster presentation at Novonordisk Foundation Metabolism in Action conference; Copenhagen, Denmark. Sparked debate.
Year(s) Of Engagement Activity 2019
 
Description BBC Radio Cambridgeshire Interview 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact Interview with BBC Radio Cambridgeshire, to discuss gut brain signalling, and the upcoming public lecture at the Science Festival
Year(s) Of Engagement Activity 2017
 
Description BBSRC symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact RF poster: Mass spectrometry vs immunoassay
SG poster: Identification and in vivo characterisation of novel gut-derived peptides
Year(s) Of Engagement Activity 2020
 
Description Big Biology Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Big Biology Day - engagement activities explaining metabolic research to families
Year(s) Of Engagement Activity 2017
 
Description CAS. Poster at EASD incretin study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact CAS. Poster at EASD incretin study group: "Tracking the development and release of hormone-containing vesicles in murine L-cells"
Year(s) Of Engagement Activity 2019
 
Description Cambridge AD HE+ Lecture 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Undergraduate students
Results and Impact lecture by RF
Year(s) Of Engagement Activity 2020
 
Description Cambridge Big Weekend 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact hands on events loosley based on the Units research
Year(s) Of Engagement Activity 2015
 
Description Cambridge City Council holiday club EM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Science activities at Cambridge City Council drop-in holiday club for families
Year(s) Of Engagement Activity 2021
 
Description Cambridge Network recruitment event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Richard Kay presented at the Cambridge Network recruitment event: "Proglucagon peptide analysis in post gastrectomy patients - bioanalysis or gut feeling?" to people interested in science jobs in the Cambridge area. (asked to present by Mike Van Der Merwe at LGC)
Year(s) Of Engagement Activity 2018
 
Description Cambridge Science Festival - Right Diet debate 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Audience reported change in views, opinions or behaviours
Year(s) Of Engagement Activity 2015
URL http://www.sciencefestival.cam.ac.uk/events/there-right-diet-me
 
Description Cambridge Science Festival - family activities 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Drop-in hands-on event for families and children. Sparked questions from children and parents
Year(s) Of Engagement Activity 2016,2017,2018
 
Description Cambridge Science Festival 2014 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Hands on activity for children sparked discussions with children and parents

Very positive feedback from public
Year(s) Of Engagement Activity 2014
 
Description Cambridge Science Festival Public Lecture 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Public Lecture: Gribble and Fletcher. "Inside out: how the gut changes the brain's view of the world". Followed by questions from the public
Year(s) Of Engagement Activity 2017
 
Description Cambridge Science festival, family weekends 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact promoted interest in science taking place at the inst
Year(s) Of Engagement Activity 2013,2014,2015
 
Description Cambridgeshire Girlguiding Girls in STEM event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other audiences
Results and Impact Cambridgeshire Girlguiding Girls in STEM event
Year(s) Of Engagement Activity 2020
 
Description Careers day at Presdales Secondary School (Cheryl Brighton) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Speed-dating job careers advise sparked interesting and stimulating discussions.

Some pupils expressed interest in a career in Science - to early to judge longterm impact.
Year(s) Of Engagement Activity 2014
 
Description DG Poster at EASD study group 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "Developing 2D organoid cultures to study intracellular signalling in enteroendocrine cells"
Year(s) Of Engagement Activity 2017
 
Description DG Poster at EASD study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact DG. Poster at Incretin Study Group: "Modelling human enteroendocrine cells using adult intestinal organoids"
Year(s) Of Engagement Activity 2019
 
Description DG. Poster at Copenhagen Biosciences Conference on Intestinal Organoids 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "Modelling hormone secretion in human enteroendocrine cells using adult intestinal organoids"
Year(s) Of Engagement Activity 2019
 
Description Diabetes UK Aug 2014 (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Participants in your research and patient groups
Results and Impact Discussed new treatments for diabetes and their benefits and limitations

Patients expressed interest that we are performing research that might improve future therapies
Year(s) Of Engagement Activity 2014
 
Description Diabetes UK July 2014 Huntingdon group (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Participants in your research and patient groups
Results and Impact Many questions answered about diabetes treatments

Patients better informed about diabetes treatments
Year(s) Of Engagement Activity 2014
 
Description EASD poster 2013 (AEA) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Interesting discussions around data presented on poster

Interesting discussion and future collaborations discussed
Year(s) Of Engagement Activity 2013
 
Description EASD poster 2013 (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Triggered interesting discussion
Year(s) Of Engagement Activity 2013
 
Description EASD poster 2014 (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Triggered interesting discussion and collaboration

Triggered interesting discussion
Year(s) Of Engagement Activity 2014
 
Description EB. Poster at Academic Foundation Programme and NIHR BRC and BTRU Trainee's Annual Research Day 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact "Localisation and characterisation of glucose-dependent insulinotropic polypeptide receptor (GIPR) expressing cells"
Year(s) Of Engagement Activity 2018
 
Description EB. Poster at EASD annual professional meeting 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "GLP1R antibody development and characterisation"
Year(s) Of Engagement Activity 2017
 
Description EB. Poster at EASD incretin study group 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "Development of a GLP-1R antagonistic antibody"
Year(s) Of Engagement Activity 2017
 
Description EM Talk at Society for Endocrinology conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Talk at Society for Endocrinology conference
Year(s) Of Engagement Activity 2021
 
Description EM. Poster at Copenhagen Bioscience Conference on Intestinal Organoids 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact EM Poster. "Using human duodenal organoids to study motilin production and secretion"
Year(s) Of Engagement Activity 2019
 
Description EM. Poster at EASD incretin study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact EM. Poster at EASD incretin study group. "The contribution of TRP channels to L-cell signalling"
Year(s) Of Engagement Activity 2019
 
Description EM: Danish Diabetes Academy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Poster presentation at Danish Diabetes academy - "Characterising stimulus-secretion coupling of the gut hormone motilin in human duodenal organoids"
Year(s) Of Engagement Activity 2020
 
Description EM: Physiology of Obesity: From Mechanisms to Medicine 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Hormonal Regulation of Gut and Pancreas Physiology" - online webinar as part of Journal of Physiology 'Physiology of Obesity: From Mechanisms to Medicine' series
Year(s) Of Engagement Activity 2020
 
Description EM: Virtual Q&A at the Cambridge Rare Disease Network RAREfest 2020 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Virtual Q&A at the Cambridge Rare Disease Network RAREfest 2020
Year(s) Of Engagement Activity 2020
 
Description FG Foster talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact FG gave invited lecture in the Department of Physiology, Development and Neuroscience at University of Cambridge.
Year(s) Of Engagement Activity 2021
 
Description FG talk at MRC-DTP symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact FG gave invited lecture at the annual symposium of the MRC-DTP students in Cambridge.
Year(s) Of Engagement Activity 2021
 
Description FG talk at Milner Therapeutics Institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact FG gave invited lecture to the Milner Institute, which includes industrial audiences from around the world. The talk triggered many interesting questions.
Year(s) Of Engagement Activity 2021
 
Description FG, BBC Horizon Special - Right Diet for You 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact 3-part BBC2 horizon series
Year(s) Of Engagement Activity 2015
 
Description FG, BBC Radio 4 programme, Inside Health 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact 'Discussion of publication: "Diagnosis of gestational diabetes: falling through the net"
Year(s) Of Engagement Activity 2015
 
Description FG, Press release, article covered by BBC News and multiple international medical publications 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact 'Discussion of publication: "Diagnosis of gestational diabetes: falling through the net"
Year(s) Of Engagement Activity 2015
 
Description FR SSIB annual meeting 2019 - poster presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact SSIB annual meeting 09-13Jul2019 (poster): "Classification of colonic enteroendocrine cells based on single cell mRNA expression analysis"
Year(s) Of Engagement Activity 2019
 
Description FR oral presentation at EASD annual meeting 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact oral presentation "GLP-1 secreting enteroendocrine cells co-release ATP to modulate vagal afferent neuronal activity" delivered on 05Oct2018
Year(s) Of Engagement Activity 2018
 
Description FR oral presentation at Wellcome Trust meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Wellcome Meeting at Sanger 31Jan-01Feb2019 (oral): "A role of hypothalamic glucose-dependent insulinotropic polypeptide receptor (GIPR) in feeding behaviour"
Year(s) Of Engagement Activity 2019
 
Description FR. Poster at EASD incretin study group. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact FR poster at EASD study group: "Classification of colonic enteroendocrine cells based on single cell mRNA expression analysis"
Year(s) Of Engagement Activity 2019
 
Description FR. presentation at Danish Diabetes Academy Winter School for postdoctoral researchers, at Higuerõn Hotel in Malaga/Spain 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "Incretins along the gut-pancreas-brain axis".
Year(s) Of Engagement Activity 2019
 
Description FR: Mercodia Diagnostics, Uppsala. Invited speaker 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Invited to talk to Mercodia about how our mass spec analysis methods complement their immunoassay development
Year(s) Of Engagement Activity 2019
 
Description Familial Gastric Cancer Study Day (Geoff Roberts) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Patients, carers and/or patient groups
Results and Impact 80-100 participants and family members, with familial gastric cancer, attended a study day to learn more about current research and practice. Geoff Roberts was one of the invited speakers. A number of patients expressed an interest in participating in our future research
Year(s) Of Engagement Activity 2016
 
Description GPR. Cambridge UGI cancer symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact "The post-gastrectomy patient, metabolic considerations"
Year(s) Of Engagement Activity 2018
 
Description GPR. Talk at EASD incretin study group meeting 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "Total Gastrectomy with Roux-en-Y reconstruction results in significant changes in gut hormone and glucose homeostasis"
Year(s) Of Engagement Activity 2017
 
Description IMS online academy EM CAS OW 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact developed work experience programme for ~12 Y10 students from underrepresented schools in Cambridge
Year(s) Of Engagement Activity 2021
 
Description Invited seminar at Genomics Institute of Novartis Research Foundation (FMG) 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Discussion about Novartis' drug development program

The researchers at Novartis used my expertise to help in their ongoing drug development program targeting intestinal enteroendocrine cells
Year(s) Of Engagement Activity 2014
 
Description Invited seminar at Technische Universität München (FMG) 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Sparked discussion and potential collaboration
Year(s) Of Engagement Activity 2013
 
Description Invited seminar at UCL (FMG) 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Triggered discussion and further experiments that we tried together

Further joint experiments following discussions on the day
Year(s) Of Engagement Activity 2014
 
Description Invited talk at Paris Descartes (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact The speaker, P.Richards, was offered a research position on the basis of this talk

Career progression for P.Richards
Year(s) Of Engagement Activity 2014
 
Description JL Choosing your first postdoc. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Lewis JE and Joanisse D (2021) Choosing your first postdoc. Career Progression Workshop, Future Physiology, online.
Year(s) Of Engagement Activity 2021
 
Description JL Oral presentation at BSN/SFE, online 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Lewis JE, Woodward ORM, Phillips BU, Tripodi M, Hornigold D, Baker D, Gribble FM, Reimann F (2021) Relaxin/insulin-like family peptide receptor 4 (Rxfp4) expressing hypothalamic neurons modulate food intake and preference in mice.
Year(s) Of Engagement Activity 2021
 
Description JL. Poster at EASD incretin study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact JL. Poster at EASD study group. "RXFP4, the cognate receptor for insulin-like peptide 5, alters food preference in mice"
Year(s) Of Engagement Activity 2019
 
Description JL: Webinar series for Physiological Society 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The series sort to highlight work by early career researchers to negate some of the negative aspects of covid/lockdown.
Year(s) Of Engagement Activity 2020
URL https://www.youtube.com/watch?v=ysOgs96WXEU&list=PLlqI96GMwNGHBi3vx5On-aS5HeZvTupuy&index=12
 
Description LB. Poster at EASD incretin study group 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "Synergistic effects of concomitant activation of FFAR1 and GPBAR-1 on L-cell calcium and secretory responses"
Year(s) Of Engagement Activity 2017
 
Description MRC Centenary Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact A hands-on practical demonstration, devised and manned by researchers from our group. The practical was based on sweet taste perception and targeted at members of the public. Engaged participation and interesting discussions with the public

Well received from feedback
Year(s) Of Engagement Activity 2013
 
Description MRL Science Slam 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The presenters (a post doc and student together) won a prize for their presentation
Year(s) Of Engagement Activity 2018
 
Description MY Poster at Incretin Study Group 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Characterisation of Electrical Activity in STC-1 Cells and their relevance to study Voltage-gated Ion Channels in Primary K- and L-cells
Year(s) Of Engagement Activity 2017
 
Description MY Poster at Incretin Study Group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact MY poster at Incretin Study Group: "Amino acid sensing in K and L cells from mouse duodenal organoids"
Year(s) Of Engagement Activity 2019
 
Description MY. Oral presentation at Diabetes UK Annual Professional Conference 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Metformin alters glucose uptake and metabolism in intestinal cells
Year(s) Of Engagement Activity 2019
 
Description OW STEM for BRITAIN 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact STEM for BRITAIN is a major scientific poster competition and exhibition which has been held in Parliament since 1997, and is organised by the Parliamentary & Scientific Committee. Its aim is to give members of both Houses of Parliament an insight into the outstanding research work being undertaken in UK universities by early-career researchers. Prizes are awarded for the posters presented in each discipline which best communicated high level science, engineering or mathematics to a lay audience. The audience was made up of MPs, senior scientists, industry representatives and early career researchers.
Year(s) Of Engagement Activity 2022
URL https://stemforbritain.org.uk/
 
Description OW. Poster at EASD incretin study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact OW poster at EASD incretin study group: "Distribution of G protein coupled relaxin/insulin-like family peptide receptor-4 (RXFP4) expressing cells in the mouse brain"
Year(s) Of Engagement Activity 2019
 
Description Oxfordshire Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Audience reported change in views, opinions or behaviours
Year(s) Of Engagement Activity 2015
URL http://www.sciencefestival.cam.ac.uk/events/there-right-diet-me
 
Description PL. Poster at Incretin Study Group 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "SCFAs increase PYY but not GLP1 production in human L-cells "
Year(s) Of Engagement Activity 2017
 
Description PL. Talk at Incretin Study Group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact PL. Talk at Incretin Study Group. "Peptidomics to study enteroendocrine cells after bariatric surgery"
Year(s) Of Engagement Activity 2019
 
Description Presentation to local Brownies 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other audiences
Results and Impact RF: Girls in STEM "Why I'm a scientist"
Year(s) Of Engagement Activity 2020
 
Description Professional PhD Networking forum AgroParistech and ABIES doctoral school Doc'Avenir 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Pierre Larraufie: Professional PhD Networking forum AgroParistech and ABIES doctoral school Doc'Avenir, 2017, Round table invited speaker about positions and career after a PhD.
Year(s) Of Engagement Activity 2017
 
Description REF Poster at SymBLS 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Size Exclusion Chromatography for Measuring Free and Antibody-bound Insulin in Hiratas Patients
Year(s) Of Engagement Activity 2019
 
Description REF. Poster at DMDG open meeting 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact "Size Exclusion Chromatography for Measuring Free and Antibody-bound Insulin in Hiratas Patients"
Year(s) Of Engagement Activity 2019
 
Description REF. Poster at EASD incretin study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact REF poster at EASD study group "A multiplex method for quantifying gut hormone peptides using Mass Spectrometry"
Year(s) Of Engagement Activity 2019
 
Description REF. Poster at REID Bioanalytical Forum 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Immunoassay vs Mass Spectrometry -Measuring insulin and insulin analogues in human plasma
Year(s) Of Engagement Activity 2019
 
Description RF: Murray Edwards Graduate Symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact RF: Measuring insulin and insulin analogues in human plasma
Year(s) Of Engagement Activity 2021
 
Description RGK Proteomics Method Forum 2020 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Peptidomics: bridging the space between large and small molecule mass spectrometry
Year(s) Of Engagement Activity 2020
 
Description RGK. American Society for Mass Spectrometry (ASMS) 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Waters user group meeting, presentation:
"LC-MS analysis of plasma from patients with insulin autoimmune syndrome"
Waters MS focused user meeting (Pharmaceutical track)
Outcome: Video of talk available on Waters website, video interview on Select Science website, as well as an in-lab video interview.
Year(s) Of Engagement Activity 2019
 
Description RGK. Poster at EASD incretin study group 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact "Development of a rapid extraction method for the analysis of gut peptide hormones by LC-MS/MS"
Year(s) Of Engagement Activity 2017
 
Description RGK. Poster at EASD incretin study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster at EASD incretin study group: "Proglucagon peptide analysis - will the real peptide please stand up!"
Year(s) Of Engagement Activity 2019
 
Description RGK. Reid Bioanalytical Forum 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact "LC-MS analysis of plasma from patients with insulin autoimmune syndrome"
Meeting focussing on broad bioanalytical approaches for wide range of analytes
Outcome: Video interview by Bionalaysis zone, available on their website
Year(s) Of Engagement Activity 2019
 
Description RGK. Waters UK and Ireland Clinical User Meeting 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Waters UK and Ireland Clinical User Meeting 2019
"LCMS Analysis of Proinsulin Derived Peptides"
Waters meeting for users of MS systems in clinical environments
Year(s) Of Engagement Activity 2019
 
Description RGK: Select Science Clinical Science Symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Plasma peptidomics - an untapped resource for biomarker analysis."
Year(s) Of Engagement Activity 2020
 
Description SG. Poster at EASD incretin study group 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact SG. Poster at EASD study group. "Characterisation of novel gut-derived peptides in vivo and in vitro"
Year(s) Of Engagement Activity 2019
 
Description SG. Poster at EASD incretin study group 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact LC-MS based detection of insulin-like peptide 5 in primary colonic cultures
Year(s) Of Engagement Activity 2017
 
Description School visit (CM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact A practical demonstration and associated discussion of the gut, presented by 2 of our PhD students to pupils at a local school.

Good feedback
Year(s) Of Engagement Activity 2013
 
Description Science lesson at primary school (CLM) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Triggered interesting discussions

Greater interest in science and medicine
Year(s) Of Engagement Activity 2012
 
Description Seminar at University of Sheffield (FMG) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Interesting discussion and plans for future collaboration

The Sheffield group have employed one of our previous summer students to do a PhD, and we plan to work together on gut hormone effects on intestinal afferent nerves
Year(s) Of Engagement Activity 2014
 
Description Stimulating innovation, experiences working with industry (CLM) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Stimulated discussion
Year(s) Of Engagement Activity 2013
 
Description Suffolk Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Suffolk Science Festival
Year(s) Of Engagement Activity 2020
 
Description Summer school 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Alison Douglas Summer School, for ~30 early career researchers from around Europe. I led an interactive morning session on gut hormones.

Very positive feedback received (scored as best presentation from feedback forms)
Year(s) Of Engagement Activity 2013
 
Description Sustainable Future Acacemy 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Online event
Year(s) Of Engagement Activity 2020
URL https://www.cam.ac.uk/public-engagement/information-for-staff-and-students/the-sustainable-futures-a...
 
Description TD. Poster at EASD study group 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact TD. Poster at EASD incretin study group. "Neutral amino acid transporter: SLC6a19- linking amino acid sensing to gut hormone secretion?"
Year(s) Of Engagement Activity 2019
 
Description Tutor on the Drug Metabolism and Discussion Group (DMDG) large molecule bioanalysis course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Richard Kay was Tutor on the Drug Metabolism and Discussion Group (DMDG) large molecule bioanalysis course. A three day course held at Loughborough University, focussed on the dissemination of knowledge for developing bioanalytical methodologies for large molecule analytes.
Year(s) Of Engagement Activity 2017
 
Description Tutor on the Drug Metabolism and Discussion Group (DMDG) large molecule bioanalysis course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Richard Kay was Tutor on the Drug Metabolism and Discussion Group (DMDG) large molecule bioanalysis course. A three day course held at Loughborough University, focussed on the dissemination of knowledge for developing bioanalytical methodologies for large molecule analytes.
Year(s) Of Engagement Activity 2017
 
Description University of Cambridge & AZ science symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact SG Presentation: Utilising LC/MS to identify novel bioactive gut-derived peptides and peptidomic differences in type 2 diabetic human islets
FG Panel discussion
Year(s) Of Engagement Activity 2020
 
Description VBL talk at Incretin 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact VBL talk at EASD Incretin Study group, "Adenosine triphosphate (ATP) is released from glucagon-like peptide 1 (GLP-1) secreting cells and signals to vagal afferent neurons"
Year(s) Of Engagement Activity 2019
 
Description VBL. Poster at Cambridge Neuroscience Seminar 2019: Signalling, Sensation & Sentience 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact VBL presented a poster at a local Cambridge Neuroscience Association meeting at Robinson College on March 14th, 2019. It was called "Cambridge Neuroscience Seminar 2019: Signalling, Sensation & Sentience" and the poster title was "ATP is released from GLP-1 secreting enteroendocrine cells and signals to vagal afferent neruones". It was a valuable experience sharing our work on gut-brain signalling to the larger Cambridge neuroscience community.
Year(s) Of Engagement Activity 2019
 
Description WT PhD Studentship meeting (London 2013) (PR) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Triggered discussion
Year(s) Of Engagement Activity 2013