Unipotency of Tbx5+ embryonic cardiac progenitor cells to cardiomyocytes.
Lead Research Organisation:
Queen Mary University of London
Department Name: William Harvey Research Institute
Abstract
Heart failure is a major cause of death in industrialized countries. Although the present medicines can attenuate the symptoms to some extent, the expected outcome is still poor. Therefore a new treatment is needed as soon as possible. Since heart failure is caused by the loss or poor functioning of heart muscle cells, destroyed or damaged during a heart attack, regeneration therapy seems promising by the replenishment of the damaged heart muscle with new heart muscle cells or heart muscle stem cells (cardiac progenitor cells; CPCs) created in the laboratory. However our current knowledge is too limited to be able to create sufficiently safe and effective cells for clinical use. Thus a much better understanding of CPCs is critical to bring such regeneration therapy into the clinical arena.
It is known that CPCs are composed of two different populations known as the first and the second heart field. The first and the second heart fields are the origin of left side and the right side of the heart, respectively. A factor called Tbx5 is present in the first heart field and another two factors called Nkx2-5 and Isl1 are present in both the first and the second heart fields. CPCs positive for Nkx2-5 or Isl1 are known to give rise to heart muscle cells as well as other components of the heart. On the other hand, although CPCs positive for Tbx5, the first heart field, are known to produce heart muscle cells, whether the first heart field can also produce other cell types present in the heart is still largely unknown.
To better understand CPCs, we have been studying CPCs from the earliest stage during embryogenesis with a state-of-art technique, single cell cDNA analysis. This technique allows us to study each a single CPC one by one, which brings detailed information with a resolution that other methods have never been able to provide. According to our data, we found the novel evidence that the first heart field CPCs are initially positive only for Tbx5, then become triple positive for Nkx2-5, Tbx5 and Isl1, and subsequently become double positive for Nkx2-5 and Tbx5. To confirm conclusively this sequential change of the first heart field CPCs according to the temporal order, we initially tried to trace Tbx5-expressing CPCs in the mouse embryonic heart by deliberately labeling Tbx5-expressing CPCs permanently with a fluorescent protein, eYFP. From this pilot study, we assume that the first heart field of Tbx5-expressing CPCs produces only heart muscle cells, which is quite different from Nkx2-5-expressing or Isl1-expressing CPCs.
In this project, we will confirm whether our original assumption that Tbx5-expressing CPCs produce only heart muscle cells is true or not. We will trace Tbx5-expressing CPCs in detail with permanent eYFP label. This will enable us to consolidate the evidence of the sequential change of Tbx5-expressing CPCs as well as to clearly identify what type of the cells Tbx5-expressing CPCs give rise to. Furthermore, we aim to establish a new method to study Tbx5-expressing cardiac progenitor cells derived from mouse embryonic stem cells. Using this new method, we can further study Tbx5-expressing CPCs in vitro without using of animals, and we can test the use of Tbx5-expressing CPCs for the treatment of animal models of heart failure by the efficient enrichment of Tbx5-expressing CPCs. Thus, successful completion of this project will clearly demonstrate whether Tbx5-expressing CPCs can only provide heart muscle cells. Such novel knowledge in basic science as well as the establishment of a useful new tool for basic science study will greatly improve our understanding of heart muscle development. We strongly believe that completion of this project is one of the many steps towards the overall goal of the establishment of future regeneration therapies for heart failure.
It is known that CPCs are composed of two different populations known as the first and the second heart field. The first and the second heart fields are the origin of left side and the right side of the heart, respectively. A factor called Tbx5 is present in the first heart field and another two factors called Nkx2-5 and Isl1 are present in both the first and the second heart fields. CPCs positive for Nkx2-5 or Isl1 are known to give rise to heart muscle cells as well as other components of the heart. On the other hand, although CPCs positive for Tbx5, the first heart field, are known to produce heart muscle cells, whether the first heart field can also produce other cell types present in the heart is still largely unknown.
To better understand CPCs, we have been studying CPCs from the earliest stage during embryogenesis with a state-of-art technique, single cell cDNA analysis. This technique allows us to study each a single CPC one by one, which brings detailed information with a resolution that other methods have never been able to provide. According to our data, we found the novel evidence that the first heart field CPCs are initially positive only for Tbx5, then become triple positive for Nkx2-5, Tbx5 and Isl1, and subsequently become double positive for Nkx2-5 and Tbx5. To confirm conclusively this sequential change of the first heart field CPCs according to the temporal order, we initially tried to trace Tbx5-expressing CPCs in the mouse embryonic heart by deliberately labeling Tbx5-expressing CPCs permanently with a fluorescent protein, eYFP. From this pilot study, we assume that the first heart field of Tbx5-expressing CPCs produces only heart muscle cells, which is quite different from Nkx2-5-expressing or Isl1-expressing CPCs.
In this project, we will confirm whether our original assumption that Tbx5-expressing CPCs produce only heart muscle cells is true or not. We will trace Tbx5-expressing CPCs in detail with permanent eYFP label. This will enable us to consolidate the evidence of the sequential change of Tbx5-expressing CPCs as well as to clearly identify what type of the cells Tbx5-expressing CPCs give rise to. Furthermore, we aim to establish a new method to study Tbx5-expressing cardiac progenitor cells derived from mouse embryonic stem cells. Using this new method, we can further study Tbx5-expressing CPCs in vitro without using of animals, and we can test the use of Tbx5-expressing CPCs for the treatment of animal models of heart failure by the efficient enrichment of Tbx5-expressing CPCs. Thus, successful completion of this project will clearly demonstrate whether Tbx5-expressing CPCs can only provide heart muscle cells. Such novel knowledge in basic science as well as the establishment of a useful new tool for basic science study will greatly improve our understanding of heart muscle development. We strongly believe that completion of this project is one of the many steps towards the overall goal of the establishment of future regeneration therapies for heart failure.
Technical Summary
This project aims to study the fate and the character of Tbx5-expressing cardiac progenitor cells (CPCs). The results obtained will enable a full re-assessment of the subpopulations of CPCs and will facilitate in vitro time-lapse observation of Tbx5-expressing CPCs and the harvesting of pure Tbx5-expressing CPCs in the future.
In this project, we will use two different genetically modified animals. BAC Tbx5-CreERT2 transgene is the bacterial artificial chromosome (BAC) clone containing 210 kb of the Tbx5 gene genome locus. CreERT2 is transcribed from this transgene in the region where Tbx5 should be expressed. The combination of this BAC Tbx5-CreERT2 transgenic mice and ROSA26-eYFP Cre reporter mice allows us to genetically label embryonic Tbx5-expressing CPCs with eYFP only when tamoxifen is administered to the pregnant females.
To trace the initial Tbx5-expressing CPCs in the BAC Tbx5-CreERT2/ROSA26-eYFP embryos, we will use whole embryo culture. This model enables tighter labeling of Tbx5-expressing CPCs strictly due to easier control of TAM concentration
To identify which type of cells are derived from Tbx5-expressing CPCs, we will perform immunofluorescence on histological sections or RT-PCR for the cells derived from embryonic cell culture or ES cell culture to detect the marker genes' expression specific for the cell type.
To trace a clone of Tbx5-expressing CPCs in vitro, the cells labeled with eYFP will be isolated by Becton Dickinson Aria II Fluorescence-Activated Cell Sorting after the embryo or embryoid body is dissociated with trypsin. Individual cells will be seeded and cultured on a feeder layer in differentiation medium.
To establish a novel experimental system to trace Tbx5-expressing CPCs in vitro without using animals, we will derive ES cells from BAC Tbx5-CreERT2/ROSA26-eYFP mouse blastcysts. Derivation is carried on by culture of blastcysts on feeder cells and iSTEM (Stem Cell Sciences) medium.
In this project, we will use two different genetically modified animals. BAC Tbx5-CreERT2 transgene is the bacterial artificial chromosome (BAC) clone containing 210 kb of the Tbx5 gene genome locus. CreERT2 is transcribed from this transgene in the region where Tbx5 should be expressed. The combination of this BAC Tbx5-CreERT2 transgenic mice and ROSA26-eYFP Cre reporter mice allows us to genetically label embryonic Tbx5-expressing CPCs with eYFP only when tamoxifen is administered to the pregnant females.
To trace the initial Tbx5-expressing CPCs in the BAC Tbx5-CreERT2/ROSA26-eYFP embryos, we will use whole embryo culture. This model enables tighter labeling of Tbx5-expressing CPCs strictly due to easier control of TAM concentration
To identify which type of cells are derived from Tbx5-expressing CPCs, we will perform immunofluorescence on histological sections or RT-PCR for the cells derived from embryonic cell culture or ES cell culture to detect the marker genes' expression specific for the cell type.
To trace a clone of Tbx5-expressing CPCs in vitro, the cells labeled with eYFP will be isolated by Becton Dickinson Aria II Fluorescence-Activated Cell Sorting after the embryo or embryoid body is dissociated with trypsin. Individual cells will be seeded and cultured on a feeder layer in differentiation medium.
To establish a novel experimental system to trace Tbx5-expressing CPCs in vitro without using animals, we will derive ES cells from BAC Tbx5-CreERT2/ROSA26-eYFP mouse blastcysts. Derivation is carried on by culture of blastcysts on feeder cells and iSTEM (Stem Cell Sciences) medium.
Planned Impact
This project is a part of a larger programme of research to understand the biological nature of the cells which form the heart tissue, the cardiac progenitor cells or CPCs, during embryogenesis. Specifically, in this project we will aim to understand the fate and the character of Tbx5-expressing CPCs, whose biological nature remains largely unknown. The results obtained will add to the field of developmental biology and specifically help us further understand how the heart develops.
Heart failure is one of the major causes of morbidity and mortality in industrialized countries. Treatment for the end stage is limited and its prognosis is still poor. Thus novel, better treatments are needed. Developmental biologists/stem cell scientists are intensely interested in the biology of CPCs, because mimicking the same molecular biological events in pluripotent stem cells could produce cardiomyocytes in the laboratory efficiently which could then be used to replenish the cells in the damaged heart. However, our knowledge is still too limited to produce sufficient numbers of safe and pure cells for such transplantation. Thus better understanding normal embryonic CPCs is vital. This project is therefore timely and one of the current hot topics. Successful completion of this project will push boundaries of cutting-edge science and contribute to the reputation of UK's science community. We strongly believe that the knowledge gained in this project is a one of the many steps toward the ultimate goal of regenerative therapy of the failing heart, which will be so beneficial to human health care.
It is anticipated that the main and direct beneficiary from this research will be the applicant's immediate professional circle, that is, developmental biologists and stem cell scientists both nationally and internationally. Given that this project will reveal novel aspects of a particular sub-population of CPCs, those expressing the factor, Tbx5, the results of this project will provide us with a basis to understand the biological nature of CPCs much further. After publication in scientific journals or presentation at scientific conferences/meetings, any interested researcher can use the results from this project for their own research. Additionally, this project will bring us a novel experimental tool to visualize Tbx5-expressing CPCs, which are likely to only develop (termed unipotent) into cardiomyocytes, in vitro. Thus this novel system will lead us to a further extension of this study, to treat heart failure with Tbx5-expressing CPCs in models of heart failure. This extension of the study could easily provide a great chance to produce patentable intellectual property surrounding Tbx5-expressing CPCs, which might attract investment from industry. Through this project, the post-doctoral scientist engaged directly and his/her colleagues in our research team will receive the highest standard of training in basic research, presentation and communication skills which will enable them to become leading scientists in their own right.
Heart failure is one of the major causes of morbidity and mortality in industrialized countries. Treatment for the end stage is limited and its prognosis is still poor. Thus novel, better treatments are needed. Developmental biologists/stem cell scientists are intensely interested in the biology of CPCs, because mimicking the same molecular biological events in pluripotent stem cells could produce cardiomyocytes in the laboratory efficiently which could then be used to replenish the cells in the damaged heart. However, our knowledge is still too limited to produce sufficient numbers of safe and pure cells for such transplantation. Thus better understanding normal embryonic CPCs is vital. This project is therefore timely and one of the current hot topics. Successful completion of this project will push boundaries of cutting-edge science and contribute to the reputation of UK's science community. We strongly believe that the knowledge gained in this project is a one of the many steps toward the ultimate goal of regenerative therapy of the failing heart, which will be so beneficial to human health care.
It is anticipated that the main and direct beneficiary from this research will be the applicant's immediate professional circle, that is, developmental biologists and stem cell scientists both nationally and internationally. Given that this project will reveal novel aspects of a particular sub-population of CPCs, those expressing the factor, Tbx5, the results of this project will provide us with a basis to understand the biological nature of CPCs much further. After publication in scientific journals or presentation at scientific conferences/meetings, any interested researcher can use the results from this project for their own research. Additionally, this project will bring us a novel experimental tool to visualize Tbx5-expressing CPCs, which are likely to only develop (termed unipotent) into cardiomyocytes, in vitro. Thus this novel system will lead us to a further extension of this study, to treat heart failure with Tbx5-expressing CPCs in models of heart failure. This extension of the study could easily provide a great chance to produce patentable intellectual property surrounding Tbx5-expressing CPCs, which might attract investment from industry. Through this project, the post-doctoral scientist engaged directly and his/her colleagues in our research team will receive the highest standard of training in basic research, presentation and communication skills which will enable them to become leading scientists in their own right.
People |
ORCID iD |
| Kenta Yashiro (Principal Investigator) | |
| Ken Suzuki (Co-Investigator) |
Publications
Brouilette S
(2012)
A simple and novel method for RNA-seq library preparation of single cell cDNA analysis by hyperactive Tn5 transposase.
in Developmental dynamics : an official publication of the American Association of Anatomists
Ishida H
(2016)
GFRA2 Identifies Cardiac Progenitors and Mediates Cardiomyocyte Differentiation in a RET-Independent Signaling Pathway.
in Cell reports
Koide T
(2015)
Collagenous gastroduodenitis with recurrent gastric ulcer in 12-year-old girl.
in Pediatrics international : official journal of the Japan Pediatric Society
Kokkinopoulos I
(2016)
Cardiomyocyte differentiation from mouse embryonic stem cells using a simple and defined protocol.
in Developmental dynamics : an official publication of the American Association of Anatomists
Kokkinopoulos I
(2015)
Single-Cell Expression Profiling Reveals a Dynamic State of Cardiac Precursor Cells in the Early Mouse Embryo
in PLOS ONE
Liu L
(2011)
Retinoic acid signaling regulates Sonic hedgehog and bone morphogenetic protein signalings during genital tubercle development
in Birth Defects Research Part B: Developmental and Reproductive Toxicology
Narita T
(2013)
The use of scaffold-free cell sheet technique to refine mesenchymal stromal cell-based therapy for heart failure.
in Molecular therapy : the journal of the American Society of Gene Therapy
Narita T
(2013)
The use of cell-sheet technique eliminates arrhythmogenicity of skeletal myoblast-based therapy to the heart with enhanced therapeutic effects.
in International journal of cardiology
Saba R
(2019)
Endocardium differentiation through Sox17 expression in endocardium precursor cells regulates heart development in mice.
in Scientific reports
| Title | BAC Tbx5-CreERT2 transgenic mice |
| Description | This mice express CreERT2 transgene according to the genomic context of transcription factor Tbx5, which enables us to trace Tbx5-expressing cells in vivo and in vitro. |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Provided To Others? | No |
| Impact | This mice are now used for tracing Tbx5-expressing cardiac progenitor cells to understand their behavior according to the context of embryogenesis. |
| Title | Direct gemome editing transient assay with CRISPR/Cas9 |
| Description | We have synthesized sgRNA for mouse genes of our interest and Cas9 mRNA in vitro, and injected into the fertilised eggs. Then the injected eggs were allowed to develop up to embryonic day 8.5. These embryos were examined in terms of the heart development as well as whether genes of our target were successfully ablated. |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Provided To Others? | No |
| Impact | This technique has been established in the USA's group, but nobody has reported that this technique is also useful for "F0 assay". This means, in some case, we do not need to generate a new mouse GM line to see the result of gene targeting. It is still useful for observing the effect of gene targeting in the case if haploinsufficiency of the gene of interest cause embryonic lethality. |
| Title | Simple sample preparation of single cell cDNA compatible with Illumina Genome Analyzer. |
| Description | One of the problems of single cell cDNA for next generation sequencing is the available amount of DNA. Additionally stably reproducible method to make short length libraries is desirable. We have developed a novel method to prepare the libraries with Nextera preparation kit (Epicentre) from only 50 ng of single cell cDNAs. |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | Easy and reproducible handling of tiny amount of cDNA, including single cell cDNA, is available with this technique for next generation sequencing in Illumina's platform. |
| Title | simple protocol to induce cardiac differentiation in mouse ES cells as 2-dimension culture |
| Description | The induction of cartdiomyocyte differentiation fromm pluripotent stem cells are usually dependent on embryoid body formation. However, the yield of cardiac cells are varied among the batches, because the size of the embryoid bodies significantly affetcs the effectiveness of the critical growth factors required for cardiac differentiation. We developed two dimension culture system to induce cardiac differentiation with a defined growth factors independent of embryoid body formation, which allows the cells in culture are exposed to the growth factors uniformly and results in the stable yield of cardiac cells among the different batches. |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Provided To Others? | No |
| Impact | The protocol is simple and feasible for many laboratories, and thus far, the yield of cardiac cells from pluripotent stem cells looks stable, which reduces our redundant efforts and to save our time and labour. |
| Title | Single-cell expression profiles of mouse embryonic cardiac progenitor cells |
| Description | This is the database of single cell level expression profiles generated from single-cell RNA-seq on mouse embryonic cardiac progenitor cells. |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | This is the firstly available single cell expression profiles of mouse embryonic cardiac progenitor cells with next generation sequencer. Thus, the deepness of the data is superior to the other available datasets. |
| URL | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE63796 |
| Title | single cell expression profile of mouse ES cells |
| Description | This is the result of single-cell expression profiling with next generation sequencing of Illimina's platform. The dataset was used to validate our original protocol of single cell expression profiling. |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | The dataset benefits the scientific community because the data indicates the feasibility, technical easiness, simplicity of the methodology, and effectiveness of our original protocol. |
| URL | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE38198 |
| Description | Elucidation of the character of human cardiac progenitor cells from human ES/iPS cells. |
| Organisation | Queen Mary University of London |
| Department | William Harvey Research Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Using human ES cells and/or iPS cells, we have elucidate the character of human cardiac progenitor cells. |
| Collaborator Contribution | They have shared us with their yuma pluripotent stem cells as a resource, appropriately under the license of UK Steering Committee to characterise human cardiac progenitor cells. |
| Impact | We have confirmed that a novel surface antigen we found, which is specific for cardiac progenitor cells in mice, is also specific for those in human. And we have also confirmed that we can isolate human cardiac progenitor cells with an antibody specific for this antigen. This means this molecule has a great impact on a future clinical application for stem cell therapy and/or pharmacological usage. We are considering to secure the patent on this issue. |
| Start Year | 2015 |
| Description | One-step gene targeting with injecting CRISPR/Cas9 RNAs into zygotes with electroporation. |
| Organisation | Osaka University |
| Department | Graduate School of Frontier Biosciences |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | We found a novel specific surface antigen for the embryonic cardiac progenitor cells. And to study the physiological function of it for the heart development, we synthesised the relevant sgRNA and Cas9 mRNA for electroporation into the mouse fertilised eggs, and examined the phenotype of the gene targetted embryos. |
| Collaborator Contribution | The collaboration contributed to induce the synthesised sgRNA and Cas9 mRNA into the fertilised eggs, which technique is not available inside the UK. |
| Impact | The heart defects were observed in the mutant mice of gene of interest. This phenotype was confirmed only within a couple of months since this gene targeting was intended, which indicates that this method is effective in saving the time and the cost. |
| Start Year | 2015 |
| Description | One-step gene targeting with injecting CRISPR/Cas9 RNAs into zygotes. |
| Organisation | Medical Research Council (MRC) |
| Department | MRC National Institute for Medical Research (NIMR) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Our group has provided the contributor with pilot data of a challenging research method, which is beneficial to fully establish a new experimental system. |
| Collaborator Contribution | NIMR has compensate the lack of requierd infrustructures in the institute where we are. |
| Impact | 1. We have already confirmed that the injection of CRISPR/Cas9 RNAs into the mouse zygotes successfully generated GM mouse embryos. In addition, this technique is useful not only for generating GM mouse line but also for F0 assay. 2. PSS of NIMR is also performing their internal service of direct genome editing with CRISPR/Cas9 so that they can collect the data of feasibility and efficiency with this novel technique from this collaboration, which benefits them and NIMR. |
| Start Year | 2014 |
| Description | 120th Annual Meeting of Japan Paediatric Society |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | This is the largest scientific meeting on Paediatrics in Japan, which over 1,000 Paediatricians attends regularly. I gave my talk on our recent work in a symposium in this meeting. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www2.convention.co.jp/120jps/index.html |
| Description | 14th London Heart Development Meeting (London) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Our group's post-doc, Dr Ioannis Kokkinopoulos gave his talk on his recent achievement in his scientific research. We could have many questions and exchange our thought frankly. We could successfully dissemniate our recent achievement in the UK's scientific community relevant to our expertise. |
| Year(s) Of Engagement Activity | 2014 |
| Description | 215th Clinical seminar series in Osaka Medical Center and Research Institute for Maternal and Child Health |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Keynote/Invited Speaker |
| Geographic Reach | Local |
| Primary Audience | Health professionals |
| Results and Impact | Title; Novel findings in the study of earliest cardiac progenitor cells by single-cell expression profiling. Date; 13th May 2013 Location; Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan Authors; Kenta Yashiro PI gave his talk in front of the clinicians who mainly are mainly engaged in children's health care. PI could discussed the importance of his scientific approaches and future directions with clinicians in terms of clinical point of view. |
| Year(s) Of Engagement Activity | 2013 |
| Description | 6th Young Embryologist Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | This meeting is held volunteerily domestic PhD students and post-docs who are interested in Developmental Biology in the UK. Our group's post-docs gave their presentations and attracted many attendants' interest to our research, and they could communicated with the young future scientists in the UK's scientific community. The young future scientists in our group has been inspired by the attendants of the same generation, and had a good opportunity to consider their own career paths. |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://www.youngembryologist.org/seminarsandevents.htm |
| Description | 8th Aso International Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Keynote/Invited Speaker |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Title; Single cell level analysis of embryonic cardiac progenitor cells. Authors; Kenta Yashiro Date; 17th May 2013 Location; Aso, Kumamoto, Japan I was invited to give my talk in this international meeting. The audiences were approximately 300 people. I have communicated with many researchers/post-docs/PhD students from UK, USA, EU and Japan. Many researchers were interested in our research and we could exchange our own ideas for basic science research and/or future potent collaboration. |
| Year(s) Of Engagement Activity | 2013 |
| Description | Attending 17th London Heart development Meeting in Institute of Child health, UCL, London (UK) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Kenta Yashiro gave his talk to disseminate the latest achievement of the scientific research funded by MRC on 14th Dec 2015 in Institute of Child Health, UCL, London, UK. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Attending Amsterdam Cardiovascular Development Meeting 2015 05 November - 06 November 2015, Amsterdam - Netherlands |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Amsterdam Cardiovascular Development Meeting 2015 05 November - 06 November 2015, Amsterdam - Netherlands The dedicated post-doc presented the latest achievement funded by MRC and exchanged the scientific opinions. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Attending to 17th International Congress of Developmental Biology (Cancun, Mexico) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | International Society of Developmental Biologists 17th International Congress of Developmental Biology Cancun, Mexico June 16 -20, 2013 Presentation of our recent achievement funded by MRC Research Grant. Many of the people in the audience were interested in our study and we could exchange scientific thoughts each other. |
| Year(s) Of Engagement Activity | 2013 |
| URL | http://www.inb.unam.mx/isdb/ |
| Description | Attending to BSDB/BSCB Spring meeting 2015 at University of Warwick (UK) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | British Society for Cell Biology British Society for Developmental Biology Joint Spring Meeting 10th - 13th April 2016 University of Warwick Oral presentation by Dr Kenta Yashiro;"Single-cell Expression Profiling Reveals The Unique Character of Tbx5-expressing Cardiac Precursor Cells of The First Heart Field in The Early Mouse Embryo." |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://thenode.biologists.com/bsdbbscb-spring-meeting-2015-report/events/ |
| Description | Consortium of Biological Science in Japan, 2017 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | This is the largest scientific meeting of molecular biology and biochemistry which over 2,000 participants attend. I gave my talk on our recent work in a workshop relevant to cardiovascular development, and interacted with many researchers, which made me inspired a lot. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www2.aeplan.co.jp/conbio2017/english/index.html |
| Description | From Stem Cells to Human Development (Surrey, UK) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | We gave our presentations and had some critical feedbacks which improves our research quality and we could successfully attract many researchers' interest to our original research. In addition, we have been inspired how to proceed stem cell scientific research in terms of human development. In this workshop, many examples of the research on human pluripotent stem cells and on "organoids" which has high potential to replace the usage of animals in the basic science were indicated. Such knowledge is critically useful for the idea of future research directions. In addition, we have successfully established a new research netwroks. |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://workshops.biologists.com/workshop-archive/workshop-september-2014/ |
| Description | Giving a talk in 18th London Heart development Meeting at ICH, UCL, London on 15th July 2016 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | -To disseminate the recent achievement in our lab -To have scientific criticism on our research -To exchange a scientific idea |
| Year(s) Of Engagement Activity | 2016 |
| Description | Giving my talk in The Institute of Medical Sciences, The University of Aberdeen on 2nd July 2016 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | -To disseminate our recent achievement -To have scientific criticism on our research -To discuss a future collaboration |
| Year(s) Of Engagement Activity | 2016 |
| Description | H3 Symposium Cellular approaches for cardiac repair; a physiological perspective, London, 2014. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | The post-doc, dedicated to MRC RG project gave his presentation in this scientific meeting organised by The Physiological Society. He could successfully attract the interest/attention from the audience. |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://www.physoc.org/h3symposium/search/google?cx=012917543776782686224%3A2zccs8ymtk0&cof=FORID%3A1... |
| Description | Hellenic Medical Society; Papanikolaou Symposium, 2014. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | The post-doc, dedicated to MRC RG project, gave his scientific presentation. He successfully attracted the interest from the audience. |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://www.hellenicmedicalsocietyuk.org/ |
| Description | Mouse Molecular Genetics |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Poster Presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Title; The study of the first heart field cardiac progenitor cells via ES cell derivation. Date; 18th-21st September 2013 Location; Sanger Institute, Cambridge, UK Authors; Ioannis Kokkinopoulos, Rie Saba, Hidekazu Ishida (Queen Mary UoL, London, UK), Hiroshi hamada (Osaka University, Osaka, Japan), Ken Suzuki, and Kenta Yashiro (Queen Mary UoL, London, UK) The post-doc supported by MRC, Dr Ioannis Kokkinopoulos successfully presented his recent results. He could communicate with many experts including Professor Margaret Buckingham. The post-doc supported by MRC, Dr Ioannis Kokkinopoulos had feedback on his study from many internationally recognized researchers, including Professor Margaret Buckingham, which mad him inspired very much. This was a good opportunity for him to experience a scientific presentation on his study. |
| Year(s) Of Engagement Activity | 2013 |
| URL | https://registration.hinxton.wellcome.ac.uk/display_info.asp?id=372 |
| Description | Mouse Molecular Genetics |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Poster Presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Title; Cell-autonomous function of SoxF proteins in the establishment of endocardial cell lineage. Date; 18th-21st September 2013 Location; Sanger Institute, Cambridge, UK Authors; Rie Saba, Ioannis Kokkinopoulus, Hidekazu Ishida, Ken Suzuki, and Kenta Yashiro (Queen Mary UoL, London, UK) The post-doc supported by BHF, Dr Rie Saba, presented successfully our recent results of BHF project. The post-doc supported by BHF, Dr Saba could communicate with many internationally recognized reseachers, which mad her inspired. And this was a good opportunity for her to present her research data by herself. She obtained good feedback on her study from the experts, including Professor Margaret Buckingham, Professor Heiko Lickert, Professor Phil Soriano and Dr Kat Hadjentonakis. |
| Year(s) Of Engagement Activity | 2013 |
| URL | https://registration.hinxton.wellcome.ac.uk/display_info.asp?id=372 |
| Description | Mouse Molecular Genetics |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Keynote/Invited Speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Title; The novel findings in single-cell expression analyses of embryonic cardiac progenitor cells. Date; 18th-21st September 2013 Location; Sanger Institute, Cambridge, UK Authors; Kenta Yashiro, Ioannis Kokkinopoulus, Rie saba, Hidekazu Ishida (Queen Maru UoL, London, UK), Hiroshi Hamada (Osaka University, Osaka, Japan), and Ken Suzuki (Queen Mary UoL, London, UK) Pi gave his talk on his lab's recent achievement. We cpuld interact with many internationally-recognized scientists and exchange ideas. One of the important impact on our research was that we could have the feedback from Professor Margaret Buckingham who has led the science on the heart development so far. Every post-doc of the PI's research team could interact with famous researchers and had been inspired with this opportunity. |
| Year(s) Of Engagement Activity | 2013 |
| URL | https://registration.hinxton.wellcome.ac.uk/display_info.asp?id=372 |
| Description | Osaka Advanced Paediatric Medicine Seminar |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Keynote/Invited Speaker |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Title; Novel findings in the study of earliest cardiac progenitor cells by single-cell expression profiling. Date; 14th May 2013 Location; Osaka University Medical School Authors; Kenta Yashiro PI gave the recent achievement in his lab in front of many clinical cardiologists, basic scientists, and PhD students. Pi had the communication with many researchers in Osaka University Medical School to exchange own ideas and disseminate our achievement. |
| Year(s) Of Engagement Activity | 2013 |
| Description | Presenting our research achievement in Keystone Symposium Cell Biology of the Heart in 2015 (USA). |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Keystone Symposium Cell Biology of the Heart: Beyond the Myocyte-Centric View (X2) Organizer(s) Peter Kohl, Robert G. Gourdie and Stefanie Dimmeler March 1-6, 2015 Copper Mountain Resort • Copper Mountain, Colorado USA Dr Ioannis Kokkinopoulos presented our research achievement funded by MRC Research Grant. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www.keystonesymposia.org/index.cfm?e=Web.Meeting.Program&Meetingid=1318 |
| Description | Seminar Series in Department of Veterinary Medical Sciences, The University of Tokyo |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Keynote/Invited Speaker |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Title; Novel findings in the study of earliest cardiac progenitor cells by single cell expression profiling. Date; 9th May 2019 Location; Department of Veterinary Medical Sciences, The University of Tokyo, Tokyo, Japan Authors; Kenta Yashiro PI gave his talk on the recent achievement in his lab. PI could communocate with many young Japanese PhD students. Pi could have discussion with his collaborator, Dr Yoshiakira Kanai in terms of SoxF class transcription factor in the heart development. |
| Year(s) Of Engagement Activity | 2013 |
| Description | The 1669th Biological Symposium in National Institute of Genetics, Japan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Keynote/Invited Speaker |
| Geographic Reach | Local |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Title; Single-Cell Expression Analyses of embryonic cardiac progenitor cells. Date; 17th July 2013 Location; National Institute of Genetics, Mishima, Japan Authors; Kenta Yashiro PI gave his talk on his lab's recent achievement. PI could communicate with many basic scientists in the institute. Of note, he discussed in person with his collaborator, Professor Yumiko Saga on the project relevant to SoxF transcription factors supported by BHF. |
| Year(s) Of Engagement Activity | 2013 |
| URL | http://www.nig.ac.jp/seminar/index-j.html |
| Description | The 24th Annual Meeting of the Japanese Society of Paediatric Myocardial Diseases in Osaka (Japan) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | The 24th Annual Meeting of the Japanese Society of Paediatric Myocardial Diseases 24th October 2015 Congrès Convention Center, Osaka, Japan Oral presentation by Dr Kenta Yashiro; "Single-cell Expression Profiling Reveals Novel Findings of Cardiac Progenitor Cells in The Early Mouse Embryo." Many people among the audience were interested in our research achievement and we had many questions. In addition, we could exchange the opinions on cardiac progenitors each other. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://med-pmd.jp/jspmd24/gaiyou.html |
| Description | The Young Life Scientists' Symposium 2013 - "Cardiovascular Medicine: Bridging Basic and Clinical Researchers" |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Poster Presentation |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Title; Cell-autonomous function of SOXF proteins in the establishment of endocardium cell lineage. Date; 6th September 2013 Location; London Authors; Rie Saba, Ioannis Kokkinopoulos, Hidekazu Ishida, Ken Suzuki, and Kenta Yashiro (Queen Mary UoL, London, UK) The post-doc funded by BHF, Dr Rie Saba, gave her presentation in this meeting. She successfully appealed our achievement in the domestic scientific community. From the educational point of view, this was a good chance to a young post-doc to give hear presentation and communicate with may researchers, which benefits her to become an independent researcher. |
| Year(s) Of Engagement Activity | 2013 |
| URL | http://www.younglifescientists2013.com/ |
| Description | The Young Life Scientists' Symposium 2013 - "Cardiovascular Medicine: Bridging Basic and Clinical Researchers" |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Poster Presentation |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Title; Lineage trace of the first heart field cardiac progenitor cell during cardiogenesis. Date; 6th September Location; London, UK Authors; Ioannis Kokkinopoulos, Rie Saba, Hidekazu Ishida (Queen Mary UoL, London, UK), Hiroshi Hamada (Osaka University, Osaka, Japan), Ken Suzuki1 & Kenta Yashiro (Queen Mary UoL, London, UK) The dedicated post-doc, Dr Ioannis Kokkinopoulus (funded by MRC) gave his presentation and successfully appealed our achievement in the domestic scientific community. The post-doc funded by MRC, Dr Ioannis Kokkinopoulus had a good chance to communicate with many scientists, which benefits him to train himself as a scientist. |
| Year(s) Of Engagement Activity | 2013 |
| URL | http://www.younglifescientists2013.com/ |
| Description | Visiting Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Head of the Department of Cell and Tissue Engineering, Cardiovascular Tissue Engineering in Stuttgart (Germany) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Kenta Yashiro visited Prof. Dr. Katja Schenke-Layland in Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Head of the Department of Cell and Tissue Engineering, Group Leader, Cardiovascular Tissue Engineering, Nobelstraße 12, 70569 Stuttgart, Germany to exchange the research information and opinions and to discuss the future collaboration. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www.schenke-layland-lab.com/ |
| Description | Visiting Osaka University Medical School (Osaka, japan). |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Visiting Osaka University Medical School on 22nd Oct 2015; (1) To give the research talk by Dr Kenta Yashiro to disseminate the latest research achievement funded by MRC Research Grant. (2) To visit professor Hiroshi Hamada, Developmental Genetics in Graduate School of Frontier Biosciences, Osaka University to exchange the scientific opinions and to discuss the potent future collaboration. (3) To visit professor Hiroshi Sasaki, Embryogenesis in Graduate School of Frontier Biosciences, Osaka University to exchange the scientific opinions and to discuss the potent future collaboration. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www.fbs.osaka-u.ac.jp/eng/general/lab/131/ |
| Description | Visiting professor Hiroki Kurihara in Department of Molecular Cell Biology, Graduate School of medicine, The University of Tokyo (Japan) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Kenta Yashiro visited professor Hiroki Kurihara in Department of Molecular Cell Biology, Graduate School of medicine, The University of Tokyo (Japan) on 28th Oct 2015 to present the latest research achievement funded by MRC, to exchange the scientific opinions on the heart development, and to discuss the potent future collaboration. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://bio.m.u-tokyo.ac.jp/index.html |
| Description | Visiting professor Masami Azuma-Kanai in Department of Experimental Animal Model for Human Disease, Tokyo Medical and Dental University (Japan) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Kenta Yashiro visited professor Masami Azuma-Kanai in Department of Experimental Animal Model for Human Disease, Tokyo Medical and Dental University (Japan) to present the latest research achievement funded by MRC, to exchange the scientific opinions and to discuss the potent future collaboration on 27th Oct 2015.. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www.tmd-cea.jp/eam/en/ |
| Description | Visiting professor Shunichi Takeda, The Department of Radiation Genetics, Graduate School of Medicine and faculty of Medicine, Kyoto University (Japan) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Kenta Yashiro visited professor Shunichi Takeda, The Department of Radiation Genetics, Graduate School of Medicine and faculty of Medicine, Kyoto University (Japan) on 21st Oct 2015 to exchange the scientific opinions and to discuss the potent future collaboration. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://rg4.rg.med.kyoto-u.ac.jp/ |
| Description | Visiting professor Toshihiko Ogura, Department of Developmental Biology and Neurosciences Division of Developmental Regulation Laboratory of Developmental Neurobiology, Institute of Development, Aging and cancer, Tohoku University (Japan) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Kenta Yashiro visited Peofessor Toshihiko Ogura in Department of Developmental Biology and Neurosciences Division of Developmental Regulation Laboratory of Developmental Neurobiology, Institute of Development, Aging and cancer, Tohoku University (Japan) on 19th Oct 2015 to present our research achievement, to exchange the scientific opinions on cardiac progenitors, and to discuss the potent future collaboration. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www2.idac.tohoku.ac.jp/dep/devn/english/index2.htm |
| Description | WHRI ANNUAL RESEARCH REVIEW |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Poster Presentation |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Title; Lineage trace of the first heart field cardiac progenitor cell during cardiogenesis. Date; 3rd July 2013 Location; William Harvey Research Institute, Queen Mary UoL, London, UK Authors; Ioannis Kokkinopoulos, Rie Saba, Hidekazu Ishida (Queen Mary UoL, London, UK), Hiroshi Hamada (Osaka University, Osaka, Japan), Ken Suzuki & Kenta Yashiro (Queen Mary UoL, London, UK) This was the internal symposium to present the achievement from each lab in the institute. Dr Ioannis Kokkinopoulus, the post-doc funded by MRC, successfully appealed his latest achievement in the institute. Dr Ioannis Kokkinopoulus had his good chance to communicate with many researchers in the institute, practice the scientific presentation, and appeal his recent achievement in fromt of the audience. |
| Year(s) Of Engagement Activity | 2013 |
| Description | WHRI Annual Research Review |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Title; Cell-autonomous function of SOXF proteins in the establishment of endocardium cell lineage. Date; 3rd July 2013 Authors; Rie Saba, Ioannis Kokkinopoulos, Hidekazu Ishida, Ken Suzuki, and Kenta Yashiro (Queen Mary UoL, London, UK) This was the internal symposium to present the achievement from each lab in the institute. Dr Rie Saba, the post-doc funded by BHF, successfully appealed his latest achievement in the institute. Dr Rie Saba had his good chance to communicate with many researchers in the institute, practice the scientific presentation, and appeal his recent achievement in fromt of the audience. |
| Year(s) Of Engagement Activity | 2013 |
| Description | Weinstein Cardiovascular Conference (Madrid) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | We gave our research talk and had some critical feedbacks to improve our research quality. In addition, we had exchanged our thinkings each other to find an alternative new direction. Some of the researchers offered to consider a future collaboration with our group. |
| Year(s) Of Engagement Activity | 2014 |
| URL | https://weinstein2014.cnic.es/home-weinstein.html |
| Description | William Harvey Annual Research Review 2014 (London) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Our group's post-docs presented their recent achievement in their research. They had many questions inside our medical school. Our group's post-doc had secured "Best Presentation Award" in this year. |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://www.whri.qmul.ac.uk/events-fundraising/annual-reviews-seminars |
| Description | William Harvey Day 2014 (London) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Our post-doc gave his presentation, and many of the attendants were interested in our research. Many of the medical school's researchers could know what our group has achieved scientifically thus far. |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://www.smd.qmul.ac.uk/staff&students/william-harvey-day/index.html |