Structure and function of the Pseudomonas aeruginosa type VI secretion system: On the bacteriophage trail

Lead Research Organisation: Imperial College London
Department Name: Life Sciences

Abstract

Bacterial pathogens infect humans using various strategies. They develop acute infections, which turn fatal to the host in a short period of time, but they also establish chronic infections and persist within the host over a lifetime. That is for example the case for lung infections in cystic fibrosis (CF) patients. At early stages of infection, diverse bacteria can be identified, including Staphylococcus aureus, Burkholderia cepacia or Pseudomonas aeruginosa. At later stages in the CF patient life the sole microorganism left in the lung is P. aeruginosa, which is firmly and chronically established and will lead to patient death.
P. aeruginosa is a potent pathogen involved in many infections, not only in CF patients. It is the 3rd most commonly-isolated nosocomial pathogen accounting for 10% of hospital-acquired infections, with 10,000 cases each year in UK. Infection is life threatening and the range of infection is broad, including urinary tract, respiratory system, dermatitis, soft tissue, bacteremia, bone and joint, intestine and a variety of systemic infections, particularly in immuno-suppressed patients. Treatment of P. aeruginosa infections involves combination therapies, e.g. a beta-lactam plus an aminoglycoside or a fluoroquinolone, but are not as efficient as desired. Alternative drugs (e.g., colistin) are useful against multi-resistant strains, but innovative therapeutic options for the future remain scarce, while attempts to develop vaccines have been unsuccessful.
To develop new therapies it is important to identify molecular targets, which once inhibited will compromise successful infection by P. aeruginosa. The target of choice is usually a major virulence factor. Secretion systems are molecular devices that bacteria use to release enzymes and toxins, which will contribute to colonization, host tissues degradation, persistence or immune system escape. These secretion systems are molecular complexes embedded in the bacterial envelope and are made up of a number of protein components.
The system we study is called the type VI secretion system (T6SS) comprising 13 core components. We selected this system because it is essential for P. aeruginosa to establish chronic infections and it is conserved in important bacterial pathogens. We chose to study 7 of the T6SS components (HsiABCEFGH), which we suspect form the core of the secretion machine. We have evidence that these components share similarities with components of bacterial viruses, i.e. bacteriophages. Since a lot is known on how bacteriophages work, we hope that understanding the correlation between T6SS and bacteriophage will shed light on how the T6SS works. The primary sequences for the phage and T6SS homologues have diverged to such an extent, that it is difficult to identify common ancestors. Although evolution can result in divergent primary sequences for homologous proteins, the structures are often conserved. Our goal is to determine the structures of T6SS proteins by using multidisciplinary approaches such as X-ray crystallography, NMR and cryo-electron microscopy. We will solve the structures of individual components and sub-complexes. The structures will provide molecular insights into how the T6SS machine is organised and will inform site directed mutagenesis studies aimed at assessing T6SS function and assembly. Perturbation in the T6SS machine will be assessed by a unique set of tests allowing us to probe whether the machine is properly assembled, able to transport toxins, and able to deliver toxins into target cells. This knowledge will later be used to design potential small molecule inhibitors that target the T6SS machine and by doing so alter T6SS function and prevent P. aeruginosa colonization and virulence. Since the T6SS is conserved in other bacterial pathogens our research will be applicable in the potential prevention of other bacterial diseases.

Technical Summary

The type VI secretion system (T6SS) is conserved in bacteria and is used to subvert host cell response or to kill competing bacteria.
Pseudomonas aeruginosa has 3 T6SS gene clusters. H1-T6SS is induced in mutants lacking the regulatory gene retS and could be studied in vitro. The T6SS is made of 13 core components, of which some display homologies with bacteriophage tail components. The bacteriophage injects DNA in target cells and it is proposed that the T6SS functions by making localized puncture in the bacterial envelope to allow secretion/injection of toxins. It is noteworthy that the few similarities between T6SS and bacteriophage, namely VgrG and Hcp, could only be predicted by 3D structural comparisons. Amino acid sequence comparisons fail to show that these components have greatly diverged in the course of evolution.
We predict that other T6SS components will have similarity with bacteriophage tail components. In order to document the correlation between T6SS and bacteriophage we will solve the structure of 7 T6SS components, HsiABCEFGH. The structures of these components will inform site directed mutagenesis studies which will be conducted to assess the assembly and function of the T6SS machine. Standard protein secretion assay and a newly developed bacterial competition assay will also be used.
The structures of T6SS components will be solved using primarily X-ray crystallography and NMR (where appropriate) and the individual structures are unlikely to fully elucidate the T6SS mechanism. Advances in EM instrumentation and image reconstruction methods now allow the architecture of large macromolecular assemblies to be elucidated. We will therefore use cryo-EM methods to characterize T6SS sub-complexes based on data from our HsiABCEFGH interaction network and combine these EM envelopes with high-resolution models of individual or sub-components to obtain pseudo-atomic models for the T6SS complex.

Planned Impact

The beneficiaries of this research are as follows:

1) The UK academic community with interests in molecular microbiology and infection and structural biology of supramolecular assemblies and nanomachines. The insights gained will be of use to academic researchers interested in developing and applying the general principles of bacterial secretion systems to understanding how they contribute to the molecular basis of human infections. Furthermore the evolutionary relationships between the T6SS and bacteriophage tail will be of benefit to evolutionary biologists by providing a clear example of lateral gene transfer and diversification.

2) Pharmaceutical industries and Biotech will also benefit form this research as it will potentially provide a novel therapeutic target (the T6SS) for small molecule intervention in human infections. The T6SS system is conserved within a number of human pathogens and the structural and mechanistic information produced in the research will be applicable to other human pathogens in addition to P. aeruginosa. The structural information will provide atomic resolution models that could form the basis for in silico ligand screening and inhibitor development, whilst the functional research could provide opportunities for assay development to test inhibition of the T6SS by small molecule entities. The potential development and manufacture of novel anti-infective agents by UK-based Pharma or Biotech based on targeting the T6SS will be of direct benefit to the UK economy as such therapeutics if successful would have a world-wide market.

3) Public sector health care professionals will benefit from the research in terms of an improved knowledge about the cause of P. aeruginosa infections and possible new treatment plans. P. aeruginosa is the 3rd most commonly-isolated nosocomial pathogen accounting for 10% of hospital-acquired infections, with 10,000 cases each year in UK. The development of novel therapeutic approaches would improve quality of life and health in the UK. Specialist health care workers treating cystic fibrosis patients would particularly benefit from the work as in late stage CF, the sole microorganism left in CF patient lungs is P. aeruginosa, which is firmly and chronically established and will lead to the patient death.

4) The UK knowledge-based economy will benefit from the interdisciplinary training of the PDRAs working in on the research project. Quantitative approaches will feature highly, and the outcomes arising from the integration of the various data sets will demonstrate the power of the interdisciplinary approach. Such trained RAs (and associated PhD, masters and undergraduate students) are likely to benefit the biotechnology and pharmaceutical industries, as well as the academic base in the UK. We therefore anticipate medium term economic benefits arising from a well-trained UK and international research base, reflected in maintaining internationally competitive research intensive universities and associated industries.

Publications

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Allsopp LP (2017) RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in . in Proceedings of the National Academy of Sciences of the United States of America

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Filloux A (2013) The rise of the Type VI secretion system. in F1000prime reports

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Filloux A (2016) Structural biology: Baseplates in contractile machines. in Nature microbiology

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Filloux A (2018) Multiple Structures Disclose the Secretins' Secrets. in Journal of bacteriology

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Hachani A (2014) The VgrG proteins are "à la carte" delivery systems for bacterial type VI effectors. in The Journal of biological chemistry

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Hachani A (2016) Type VI secretion and anti-host effectors. in Current opinion in microbiology

 
Description Insitute for Life Sciences (IfLS), University Southampton
Geographic Reach National 
Policy Influence Type Participation in advisory committee
 
Description International Centre for Infectiology Research" (CIRI, Lyon, France)
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
 
Description MRC board Infection and Immunity
Geographic Reach National 
Policy Influence Type Membership of a guideline committee
 
Description Max Planck Institute- Terrestrial Microbiology
Geographic Reach Asia 
Policy Influence Type Participation in advisory committee
 
Description Scientific Board - KU Leuven
Geographic Reach Asia 
Policy Influence Type Participation in advisory committee
 
Description H2020-MSCA-IF-2014. Marie Curie Fellowship to Laurent Dortet - 01/02/2016 to 31/01/2018
Amount € 195,454 (EUR)
Organisation Marie Sklodowska-Curie Actions 
Sector Academic/University
Country Global
Start 02/2016 
End 01/2018
 
Description MRC Programme grant-Structure and function of the Pseudomonas aeruginosa type VI secretion system: On the bacteriophage trail
Amount £1,768,908 (GBP)
Funding ID MR/K001930/1 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 01/2013 
End 12/2017
 
Description MRC Studentship to Panayiota Pissaridou (PP)
Amount £79,500 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 10/2014 
End 10/2017
 
Description Marie-Curie Actions-International Incoming Fellowships to Laura Nolan - PAT6SS - - 01/03/2014-28/02/2016.
Amount € 221,606 (EUR)
Funding ID PIIF-GA-2013-625318 
Organisation Marie Sklodowska-Curie Actions 
Sector Academic/University
Country Global
Start 03/2014 
End 02/2016
 
Description Marie-Curie Actions-International Incoming Fellowships to Luke Allsopp - T6SS-Pseudo Effectors - - 01/01/2014-31/12/2015.
Amount € 221,606 (EUR)
Funding ID PIIF-GA-2012-328261 
Organisation Marie Sklodowska-Curie Actions 
Sector Academic/University
Country Global
Start 01/2014 
End 12/2015
 
Description Wellcome Trust Project Grant (The fate of VgrG effector proteins in the type VI secretion process: a key issue in bacteria-host relationship)
Amount £394,173 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 09/2010 
End 08/2013
 
Description Wellcome Trust Studentship to Thomas Wood (TW)
Amount £160,280 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2014 
End 09/2017
 
Title Anti-ClpV1 
Description Antibody directed against the AAA ATPase ClpV, a core component of the T6SS 
Type Of Material Antibody 
Year Produced 2013 
Provided To Others? Yes  
Impact Collaboration with Dr Marian Llamas in Granada/Spain 
 
Title Anti-HsiA 
Description Antibodies directed against one of the component of the T6SS machinery 
Type Of Material Antibody 
Provided To Others? No  
Impact Further understanding of the assembly of the T6SS machinery 
 
Title Anti-HsiEB 
Description Antibodies against core component of the T6SS machine have been generated. It includes antibodies against HsiE and HsiB. 
Type Of Material Antibody 
Provided To Others? No  
Impact These antibodies allow to follow assembly of the T6SS components into the T6SS machinery, to follow their sub-cellular localization and to perform Co-IP to identify new interactants. 
 
Title Bacterial Killing Assay 
Description The T6SS has been proposed to provide a growth advantage to P. aeruginosa in polymicrobial infections. An assay has been set up to assess T6SS function in P. aeruginosa by following killing of competing bacteria (E. coli) 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2013 
Provided To Others? Yes  
Impact The killing assay provides a quick and easy phenotypic assessment of T6SS function and allows to screen any potential mutant affected in the T6SS assembly process. 
 
Description Academia Sinica - Taiwan 
Organisation Academia Sinica
Department Agricultural Biotechnology Research Centre
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution This is a collaboration between the laboratory of Ehr-Min Lai on the interaction between Pseudomonas aeruginosa and Agrobacterium tumefaciens and about the role of the T6SS in the competition between these two organisms.
Collaborator Contribution Expertise on Agrobacterium and on the T6SS in this organism
Impact We have co-authored a manuscript which has been released in Cell Host & Microbe in 2014 (see publications) We have secured a BBSRC partnership award and have started the exchange programme. Professro Erh-Min Lai and Dr Jer-Sheng Li visited Imperial in September 2015, whereas Prof Filloux and Sarah Wettstadt (PhD student) visited Academia Sinica in November 2015.
Start Year 2012
 
Description CSIC-Granada-Spain-2014-Marian Llamas 
Organisation Spanish National Research Council (CSIC)
Country European Union (EU) 
Sector Public 
PI Contribution Collaboration stemming from the attribution of an EMBO short term fellowship to Patricia Bernal. Patricia stayed at Imperial College London from October to December 2014. Patricia Bernal has now secured a Talent Hub fellowship and a Marie Curie fellowship so that she could stay at Imperial until 2018. This work has also been the basis for the BBSRC award number: BB/N002539/1 which will commence on March 1 2016
Collaborator Contribution Study of the T6SS in Pseudomonas Putida
Impact EMBO Short term fellowship to Patricia Bernal Talent Hub fellowship to Patricia Bernal Marie Curie fellowship to Patricia Bernal
Start Year 2014
 
Description Collaboration David Albesa-Jove (University of the Basque country) 
Organisation University of the Basque Country
Country Spain 
Sector Academic/University 
PI Contribution We provided electron microscopy data of the baseplate component of the T6SS, TssA We identified Tse8 as a GatA homologue and a Pseudomonas aeruginosa T6SS toxin
Collaborator Contribution Based on sequence data and EM images they designed a model for the 3D structure of TssA (Dodecameric rings) Based on the sequence of Tse8 and the 3D structure of GatA, we identified key residues which could explain the difference of functionality between Tse8 and GatA.
Impact We have a paper in revision at EMBO J describing the TssA structure
Start Year 2015
 
Description Collaboration Dr Serge Mostowy (Imperial College London) 
Organisation Imperial College London
Department Faculty of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution We will provide Pseudomonas aeruginosa mutant strains to assess virulence in the Zebra fish model
Collaborator Contribution Dr Mostowy will perform the infections and provide data including live imaging microscopy.
Impact Preliminary data have identified some of our mutants (warA and sadC) as being poorly targeted by the Zebra fish macrophages.
Start Year 2015
 
Description Collaboration Professor Aras Kadioglu (Liverpool University) 
Organisation University of Liverpool
Department Institute of Infection and Global Health
Country United Kingdom 
Sector Academic/University 
PI Contribution Professor Kadioglu has extensive expertise for working with mice models of infection using Pseudomonas aeruginosa, and we will provide a large set of mutant strains to assess their virulence.
Collaborator Contribution Professor Kadioglu will perform the infections and collect the data.
Impact Collaboration just started and we have provided the strains
Start Year 2015
 
Description Fluorescence microscopy -FILM at Imperial 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Visualisation of T6SS dynamic using bacteria expressing fluorescently tagged T6SS.
Collaborator Contribution Visualisation of T6SS dynamic using bacteria expressing fluorescently tagged T6SS
Impact Microscopy images have been included in the Planamente et al, 2016, EMBO Journal
Start Year 2014
 
Description MRC laboratory of Molecular Biology - Cryo-EM - Sjors Sheres 
Organisation University College London
Department MRC Laboratory for Molecular Cell Biology
Country United Kingdom 
Sector Academic/University 
PI Contribution Preapration of sample for Cryo-EM
Collaborator Contribution Cryo-EM analysis and 3D reconstruction
Impact The Cryo-EM data for the T6SS P. aeruginosa TssBC sheath are currently put together and will be submitted to nature Microbiology
Start Year 2015
 
Description Protein Production platform 
Organisation Imperial College London
Department Centre for Structural Biology
Country United Kingdom 
Sector Academic/University 
PI Contribution We will design the strategy and the target proteins to purify
Collaborator Contribution The Protein Production and Analysis (PPA) platform at the CMBI is dedicated to the optimization of gene cloning and protein purification.
Impact None yet
Start Year 2013
 
Description Statistical analysis of the spatio-temporal distribution of the T6SS in bacteria 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Engineering bacterial strains with fluorescently labelled T6SS components and recording videos
Collaborator Contribution Mathematical and statistical analysis of the T6SS foci distribution in bacterial cells and bacterial populations.
Impact Hiring a Wellcome Trust PhD student who is hosted by Niall Adams in the Department of Mathematics
Start Year 2017
 
Description Structral Biology of T6SS components 
Organisation Imperial College London
Department Centre for Structural Biology
Country United Kingdom 
Sector Academic/University 
PI Contribution Access to structural biology facilities to develop the MRC-funded project. The MRC-funded RA in my lab bridges the work of the MRC-funded RT and another technician in my lab with our collaborator working at the Centre of Structural Biology.
Collaborator Contribution Access to structural biology facilities to develop the MRC-funded project
Impact Crystals have been obtained for subcomplex of the T6SS machinery, namely HsiE/HsiB. 3D structure has been resolved for HsiE in complex with the N terminus of HsiB, and these data have been published The structure of the N terminal domain of ClpV has been obtained and published. The HsiBC sub-complex is studied by CryoEM. The structure is currently refined and the 3D model shows a ring shape-like structure displaying a 12-fold symmetry. EM images of part of the T6SS baseplate have been obtained and currently optimized
Start Year 2009
 
Description UCL Molecular Interactions Facility - Collaboration Prof S J Perkins and Mr Jayesh Gor 
Organisation University College London
Department Biosciences
Country United Kingdom 
Sector Academic/University 
PI Contribution We provided samples of the TssA protein, a baseplate component of the T6SS
Collaborator Contribution They performed Analytical Ultra Centrifugation (AUC to determine the level of oligomerisation of the complex (dodecamer).
Impact We have a pepr submitted to EMBO J reporting the structure and function of TssA, a baseplate component of the T6SS
Start Year 2015
 
Description Presentations Luke Allsopp and Ronan McCarthy at Antimicrobial Research Collaborative (ARC) launch, Imperial College, London, United Kingdom, September 25th, 2015 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation by Luke Allsopp entitled; New insights into the T6SS of Pseudomonas.
Presentation by Ronan McCarthy entitled; The Diguanylate Cyclase SadC Is a Central Player in Gac/Rsm-Mediated Biofilm Formation in Pseudomonas aeruginosa
This was at the occasion of the kick off meeting of a new network of researchers from across the College and Imperial Healthcare Trust with an interest in the global challenge of AMR.
Year(s) Of Engagement Activity 2015
URL http://www.imperial.ac.uk/arc
 
Description A Talk by Sara Planamente at the Young Microbiologist Symposium, Norwich, UK-2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Sara Planamente gave a talk on her T6SS work recently published in the EMBO Journal

Young Microbiologists Symposium at the John Innes Centre-Norwich, UK - 2016
Year(s) Of Engagement Activity 2016
 
Description A talk at NTU in Singapore by Prof Alain Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk by the PI Alain Filloux to PI, Postdoctoral fellows and PhD students from the SCELSE Institute at the NTU in Singapore.
Role of c--di-GMP in biofilm formation and immune evasion and hos it relates to the central regulatory network RsmA/SadC
Year(s) Of Engagement Activity 2016
 
Description A talk at the YMS conference in Dundee by Alain Filloux -2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk by Alain Filloux:
YMS 2016, Young Microbiologists Symposium on Microbe Signalling, Organisation and Pathogenesis. The bacterial Type VI Secretion System: Killing with moderation. University of Dundee, Dundee 29-30th June 2016
Year(s) Of Engagement Activity 2016
URL http://www.lifesci.dundee.ac.uk/other/yms/
 
Description A talk on T6SS at the ASM Microbe 2016 by Alain Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk by Alain Filloux at:
ASM Microbe 2016. Type VI Secretion System and Bacterial Toxins: A Matter of Friends or Foes Boston. Boston, USA, June 16-20, 2016.
Year(s) Of Engagement Activity 2016
 
Description ASM Conference on "Mechanisms of Interbacterial Cooperation and Competition, 2015, Washington - Talk Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I gave a talk to present our work on the T6SS in Pseudomonas aeruginosa entitled: Molecular Aspects of Pseudomonas aeruginosa T6SS Assembly and Bacterial Competition

The meeting was in Washington DC, from March 12 to 17, 2015.
Year(s) Of Engagement Activity 2015
 
Description ASM Conference on Pseudomonas 2015, Talk Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Presentation of our work on the Pseudomonas aeruginosa T6SS.

Title: The Pseudomonas aeruginosa Type VI secretion system: a weapon for bacterial warfare; Place: ASM Conference on Pseudomonas, Washington (USA), September 8-12, 2015.
Year(s) Of Engagement Activity 2015
 
Description Academia Sinica (IPMB), Taipei, Taiwan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact talk in the context of networking with our collaborator Erh-Min Lai
Year(s) Of Engagement Activity 2015
 
Description Academia Sinica, Taipei, Taiwan, 2013, Talk Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Academia Sinica (Erh-Min Lai), Taipei, Taiwan, November 4, 2013, The type VI secretion system: On the bacteriophage trail.

Talk by Filloux and further discussion on collaborative projects

Co-authored publication in Cell Host & Microbe
Year(s) Of Engagement Activity 2013
 
Description Academia Sinica, Taipei, Taiwan, Talks Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact This was a visit to establish and nurture the collaboration with the group of Professor Erh-Min Lai in the context of our BBSRC partnership award.
I gave two talks to engage with Post-docs and students and to stimulate discussion for future plans in our project.
The title of the talks were: Rewiring c-di-GMP signalling to control bacterial biofilm formation & motility AND The Pseudomonas aeruginosa Type VI secretion system: a phage-like bacterial killing device

The visit lasted from November 8 to 14, 2015
Year(s) Of Engagement Activity 2015
 
Description CMBI Open Day-2013 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact About 110 pupils attended an open day at the CMBI.
Each PI had organized a lay presentation in the form of psoters and videos to familiarize children witht he problem of bacterial infection and how to treat them.
Our presentation was focused on Pseudomonas aeruginosa and the Type VI secretion system (T6SS)

Teachers and pupils were really impressed by the world of microbes at the CMBI
Year(s) Of Engagement Activity 2013
URL http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/centres/cmbi/newssummary/news_23-10-20...
 
Description EIMID 10th annual meeting, 2014, Poster Planamente 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact EIMID 10th annual meeting, Saint-Maximin, FRANCE, 2-04 October 2013 (Poster: Novel features of the AAA+ ATPase ClpV define distinct subclasses of type VI secretion systems. Poster Sara Planamente


Well received, excellent feedback
Year(s) Of Engagement Activity 2014
 
Description European Course on Microbial Evolution and Molecular Epidemiology, ENS Lyon 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Course for for French Microbiologists at the Ecole Normale Supérieure de Lyon
Year(s) Of Engagement Activity 2015
 
Description FEMS 2015 - Maastricht, The Netherlands - Talk Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I presented our work on the Pseudomonas aeruginosa T6SS.
The conference was held in Maastricht (The Netherlands) from June 6 to 11, 2015.
Year(s) Of Engagement Activity 2015
 
Description Gordon Conference on Bacterial Cell Surfaces, 2014, Poster Planamente 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Gordon Conference on Bacterial Cell Surfaces, West Dover, VT USA, 22-27 June 2014. Poster: Coevolution of the ATPase ClpV and the B/C sheath distinguishes two type VI secretion classes. Poster Sara Planamente

Well received, excellent feedback
Year(s) Of Engagement Activity 2014
 
Description Hidden Wonders: a journey into the bacterial world: Multimedia outreach -2014 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact 14-15/03/2014 - Hidden Wonders: a journey into the bacterial world: Multimedia outreach event presenting the key features of bacteria to members of the public, at Imperial College.

School pupils were taught the importance of molecular studies in bacterial pathogens. We described the T6SS and c-di-GMP signalling in Pseudomonas aeruginosa in lay terms
Year(s) Of Engagement Activity 2014
 
Description Imperial College Press release- Interview Professor Alain Filloux 2014 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Imperial College communication released a news based on our paper published in Cell Host & Microbe (2014 Jul 9;16(1):94-104).

Broader impact of our T6SS work on the public and non expert academics and students
Year(s) Of Engagement Activity 2014
URL http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/newssummary/news_16-7-2014-11-58-1
 
Description Imperial Festival Outreach - Super Bugs Zone - Educating chirldren to the world of Microbiology 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Several members of the Filloux's group, Laura Nolan (PDRA), Patricia Bernal (PDRA), Lukke Allsopp (PDRA), Thomas Wood (PhD student), Panayiota Pissaridou (PhD student), Sophie Howard (PhD student) and Sara Planamente (PDRA), designed a stand in May 2017 for the Imperial festival. These were describing various aspects of Microbiology from recognizing a bacterium to detailed molecular mechanisms of an antibacterial weapon, the T6SS.
Year(s) Of Engagement Activity 2017
 
Description Interview Professor Filloux by Faculti Media 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Faculti Media asked for an interview following up on the release of the Cell Host & Microbe paper (2014 Jul 9;16(1):94-104). The interview lasted 20 min and will be published on the Faculti Media website.

This should impact on a large audience and publicize in lay terms our research on the T6SS.
Year(s) Of Engagement Activity 2014
URL https://facultimedia.com/downloads/category/all/natural-sciences/
 
Description Invited talks for Luke Allsopp at the London School of Hygiene and University of Kent on the T6SS 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Invited talks recognizing Luke Allsopp has a valued PDRA from the Filloux lab and highlighting his research on T6SS
Year(s) Of Engagement Activity 2017
 
Description Laura Nolan mentoring at CREST program 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact For the mentoring of British Science Association's CREST program Laura Nolan (Marie Curie Fellow) mentored 5 year 11 students over an 11 month period in order for them to obtain a CREST Gold Award. This project began at the end of 2014 but went into 2015 and ended technically in September. The program involved guiding their initial very broad idea of wanting to investigate antibiotic resistance in 'superbugs' into a research project which they would actually be able to safely and practically investigate. Initial meetings with the group allowed for development of the hypothesis that bacteria isolated from a clinical setting would be more resistant to antibiotics than bacteria isolated from the environment. To address this hypothesis we tested the intrinsic and acquired resistance to antibiotics of bacteria isolated from various environmental locations (soil outside at school, a hospital and in a remote country area) and a number of clinical P. aeruginosa, E. coli and S. aureus strains (I worked with the clinical strains of course). Experimental work was performed both at Imperial College labs as well as at their school (Twyford Church of England School). This mentoring program allowed the students to get real 'hands on' experience within a lab and of a research project, and also allowed us to showcase our facilities and to engage with the next crop of potential UK scientists about aspects of our research.
Year(s) Of Engagement Activity 2015
 
Description Lunteren 2013 -The Netherlands-Talk Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact I gave a keynote lecture to a grouping of Microbiologists in the Netherlands. The topic related to our recent work on the T6SS both structural aspects and bacterial competition/warfare.

Presentation well received.
Year(s) Of Engagement Activity 2013
 
Description Nanyang Technological University (NTU), Singapore Center for Environmental Life Sciences Engineering (SCELSE). 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact talk in the context of Networking with future collaborators in Singapore on biofilm, c-di-GMP signalling and T6SS
Year(s) Of Engagement Activity 2016
 
Description National Chung-Hsing University, Taiwan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Networking with our collaborator Erh-Min Lai in Taiwan
Year(s) Of Engagement Activity 2016,2017
 
Description Poster presentation by Thomas Wood at the FEMS 2017 meeting on the T6SS effector VgrG2b 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact PhD student from the Filloyx's group presenting work on the T6SS
Year(s) Of Engagement Activity 2017
 
Description Poster presented by Sara Planamente, Panyiota Pissaridou and Sarah Wettstadt at the Pseudomonas conference in Liverpool - The T6SS machinery and its effectors 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presentation of our work on the T6SS
Year(s) Of Engagement Activity 2017
 
Description Posters from Luke Allsopp, Thomas Wood and Sarah Wettsadt at the 5th Molecular Microbiology and 4th Midlands Molecular Microbiology Meeting in Birmingham 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact PDRA and PhD students from the Filloux lab, Luke Allsopp, Thomas Wood and Sarah Wettsadt, presented talks and poster on the T6SS
Year(s) Of Engagement Activity 2017
 
Description Presentation Laura Nolan at "Adaptation and Communication of Bacterial Pathogens Conference" (Baeza, Spain, October 25th-28th, 2015) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Lura Nolan gave a short talk on "Uncovering the mechanism of membrane puncture and the full toxin repertoire of the Type VI secretion system of Pseudomonas aeruginosa"
Year(s) Of Engagement Activity 2015
 
Description Presentation Luke Allsopp at the 6th FEMS Microbiology Congress, Maastricht, Netherlands, June 7-11, 2015 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presentation by Luke Allsopp (Marie Curie fellwo) entitled; New insights into the T6SS of Pseudomonas aeruginosa at one of the largest Microbiological Conferences.
Year(s) Of Engagement Activity 2015
 
Description Professor Filloux Chair of session on Antimicrobial Research at the ARC launch, 25th September 2015 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact At the occasion of the launch of Professor Alain Filloux chaired a session on MOLECULAR & STRUCTURAL BIOLOGY AND THERAPEUTICS (Imperial College London Antimicrobial Research Collaborative (ARC) Official Launch).
Year(s) Of Engagement Activity 2015
URL https://workspace.imperial.ac.uk/medicine/Public/Faculty%20Centre/ARC%20Launch%20Agenda%20FINAL.pdf
 
Description SGM annual conference, 2014, Poster Planamente 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact SGM annual conference, Liverpool, UK, 14-17 April 2014, Poster: Coevolution of the ATPase ClpV and the B/C sheath distinguishes two type VI secretion classes. Poster Sara Planamente

Well received, excellent feedback
Year(s) Of Engagement Activity 2014
 
Description SuperBug Zone, Imperial Festival, Imperial College London 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact On May 9-10, schools visited the MRC-CMBI and we had a super bug zone prepared for them.
Ronan MacCarthy (PDRA BBSRC grant) was the Social Media Coordinator. He co-ran the social media profiles of the Superbug Zone over the two day public outreach event communicating Bacteriology and what are Microbes to the public (young and old) and also responding to questions online.
Luke Allsopp (Marie Curie Fellow) co-ran the first stand on 'What are Bacteria'.
Imperial Festival showcases to the public, alumni, staff and students all that Imperial has to offer and contributes.
Also counts at the open day for the MRC CMBI.
Year(s) Of Engagement Activity 2015
 
Description Talk Alain Filloux Academia Sinica in Taipei 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact From January 15th, 2017 to January 23rd 2017, Alain Filloux visited Erh-Min Lai laboratory and to further discuss the collaboration and give a talk.
Year(s) Of Engagement Activity 2017
 
Description Talk by Sara Planamente at the ZING Conference 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Sara Planamente gave a talk at the ZING conference on protein secretion systems to report on her work recently published in EMBO Journal.
This is a conference entirely focussed on bacterial protein secretion systems.

Zing conference "Protein Secretion in Bacteria", November 2016-Florida,USA
Year(s) Of Engagement Activity 2016
 
Description University of Geneva, Frontiers in Biomedicine series, Talk Filloux 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact University of Geneva, Frontiers in Biomedicine series, June 5, 2014, The Pseudomonas aeruginosa type VI Secretion System:a Weapon for Bacterial Warfare

invited seminar

Well-received, excellent feedback
Year(s) Of Engagement Activity 2014
 
Description Young Microbiologists' Symposium (YMS) Dundee-2014 -Talk Allsopp 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Participants in your research and patient groups
Results and Impact Young Microbiologists' Symposium (YMS) Dundee June 2014 - The VgrG proteins of Pseudomonas aeruginosa are "A la carte" delivery systems for bacterial Type VI toxins

Talk Allsopp

Support for the development of promising young scientists in the field of microbiology and infection

Well received, excellent feedback
Year(s) Of Engagement Activity 2014