Roles of ubiquitin and SUMO during chromosomal DNA replication.
Lead Research Organisation:
University of Birmingham
Department Name: Cancer Sciences
Abstract
Cell division is the basis for the propagation of life. This involves the precise duplication of genetic information, which is called DNA replication. This process must be, and is, precisely regulated. Any mistakes that are not subsequently repaired can change the way the cell behaves and result in conditions such as genetic diseases, cancer and ageing. It is fascinating that in most cases cells can achieve this task of duplicating the whole genome precisely once and without mistakes. Genome duplication is also essential for fast dividing cancer cells. It is why many anti-cancer therapies target DNA replication, but these are so far not specific for cancer cells and have significant side-effects. It is therefore crucial that we fully understand this fundamental process so that we can develop better and more specific anti-cancer strategies.
Cells have developed many ways of dealing with damaged DNA to maintain an intact genome. Last few years brought to light the importance of small-protein modifiers called ubiquitin and SUMO in regulating key DNA repair proteins. There is strong evidence to suggest that these modifications are also important during the replication of undamaged DNA. However little is known about the proteins they are targeting. The aim of my project is to investigate the role of ubiquitin and SUMO during replication of undamaged DNA.
Two biological systems will be used to fulfill this project. The cell-free system using extracts from the eggs of the African clawed frog (Xenopus laevis) contains pre-formed complexes of most proteins required for cell cycle progression and so can support a complete round of DNA replication in a test tube. As many aspects of this process are highly similar in all eukaryotic organisms studied, the mechanisms identified in simpler egg extract model system are most often true also in human cells. Once novel modifications have been identified and basic mechanistic studies have been performed in Xenopus egg extract I would like to investigate whether analogous mechanism function also in human cells.
In particular, I am interested in investigating the role of ubiquitin during the termination stage of DNA replication, as suggested by my preliminary data. Replication termination occurs when two DNA replication forks coming from the opposite sites of the chromosome fuse together. The efficient and faultless resolution of these structures is crucial for maintaining the genome integrity. There are thousands of replication forks in human cell which have to be resolved during each termination phase - it is crucial therefore that we create tools to study this process and determine its input towards tumorgenesis. However, this stage of DNA replication is also very poorly understood and therefore a very exciting research area. I will study the mechanism by which ubiquitylation regulates this process and the consequences of its disruption. In an analogous way, I will examine the effects of blocking sumoylation on different aspects of DNA replication.
I would also like to take advantage of the simplicity of biochemical analysis of DNA replication in Xenopus system and perform a systematic analysis of proteins associating with replicating DNA and modified during DNA replication. I will then choose the most interesting ones and characterize the type and site of these modifications. My final aim is to determine the function of the identified modifications and their importance in process of DNA replication and cancer development.
Defining the role of ubiquitin and SUMO modifications during DNA replication will widen our understanding of this process. Both DNA replication and the ubiquitin system are targeted by many current anti-cancer chemotherapies. Unveiling new crosstalk pathways between these two may therefore suggest novel targets or combinations of chemotherapeutic agents as it may reveal new ways of creating DNA damage specifically lethal to cancer cells.
Cells have developed many ways of dealing with damaged DNA to maintain an intact genome. Last few years brought to light the importance of small-protein modifiers called ubiquitin and SUMO in regulating key DNA repair proteins. There is strong evidence to suggest that these modifications are also important during the replication of undamaged DNA. However little is known about the proteins they are targeting. The aim of my project is to investigate the role of ubiquitin and SUMO during replication of undamaged DNA.
Two biological systems will be used to fulfill this project. The cell-free system using extracts from the eggs of the African clawed frog (Xenopus laevis) contains pre-formed complexes of most proteins required for cell cycle progression and so can support a complete round of DNA replication in a test tube. As many aspects of this process are highly similar in all eukaryotic organisms studied, the mechanisms identified in simpler egg extract model system are most often true also in human cells. Once novel modifications have been identified and basic mechanistic studies have been performed in Xenopus egg extract I would like to investigate whether analogous mechanism function also in human cells.
In particular, I am interested in investigating the role of ubiquitin during the termination stage of DNA replication, as suggested by my preliminary data. Replication termination occurs when two DNA replication forks coming from the opposite sites of the chromosome fuse together. The efficient and faultless resolution of these structures is crucial for maintaining the genome integrity. There are thousands of replication forks in human cell which have to be resolved during each termination phase - it is crucial therefore that we create tools to study this process and determine its input towards tumorgenesis. However, this stage of DNA replication is also very poorly understood and therefore a very exciting research area. I will study the mechanism by which ubiquitylation regulates this process and the consequences of its disruption. In an analogous way, I will examine the effects of blocking sumoylation on different aspects of DNA replication.
I would also like to take advantage of the simplicity of biochemical analysis of DNA replication in Xenopus system and perform a systematic analysis of proteins associating with replicating DNA and modified during DNA replication. I will then choose the most interesting ones and characterize the type and site of these modifications. My final aim is to determine the function of the identified modifications and their importance in process of DNA replication and cancer development.
Defining the role of ubiquitin and SUMO modifications during DNA replication will widen our understanding of this process. Both DNA replication and the ubiquitin system are targeted by many current anti-cancer chemotherapies. Unveiling new crosstalk pathways between these two may therefore suggest novel targets or combinations of chemotherapeutic agents as it may reveal new ways of creating DNA damage specifically lethal to cancer cells.
Technical Summary
Post-translational modification of proteins by members of the ubiquitin family: specifically ubiquitin and SUMO, is essential in DNA damage response pathways. However their role during unperturbed DNA replication not well understood.
The initiation stage of DNA replication depends on ubiquitin-mediated proteolysis. However, a growing body of evidence suggest that the further stages of DNA replication are also regulated by these modifications. The importance of ubiquitin/SUMO ligases during unperturbed DNA replication has been shown, but very little is known about their substrates. My aim is therefore to use my experience in studying protein complexes assembled during DNA replication to investigate their regulation by ubiquitin and SUMO.
For the first part of my project I will use the cell-free Xenopus egg extract system, which provides a powerful system for the biochemical analysis of eukaryotic chromosomal replication. I will identify proteins ubiquitylated and sumoylated during DNA replication by mass spectrometry analysis. I will then select most interesting candidates and map the modifications sites and their function during DNA replication.
My preliminary data suggest an important role for polyubiquitylation in the disassembly of the replisome upon termination of DNA replication forks. These findings are of the highest interest as at present we have little knowledge of this process. I will, therefore, study the mechanism and the consequences of this defect. I will also investigate the effects of blocking sumoylation on replisome formation, regulation and disassembly.
Once novel ubiquitylated and sumoylated proteins have been identified and basic mechanistic studies have been performed using Xenopus egg extracts I will select a few most interesting substrates and study their modifications in human cell lines.
The initiation stage of DNA replication depends on ubiquitin-mediated proteolysis. However, a growing body of evidence suggest that the further stages of DNA replication are also regulated by these modifications. The importance of ubiquitin/SUMO ligases during unperturbed DNA replication has been shown, but very little is known about their substrates. My aim is therefore to use my experience in studying protein complexes assembled during DNA replication to investigate their regulation by ubiquitin and SUMO.
For the first part of my project I will use the cell-free Xenopus egg extract system, which provides a powerful system for the biochemical analysis of eukaryotic chromosomal replication. I will identify proteins ubiquitylated and sumoylated during DNA replication by mass spectrometry analysis. I will then select most interesting candidates and map the modifications sites and their function during DNA replication.
My preliminary data suggest an important role for polyubiquitylation in the disassembly of the replisome upon termination of DNA replication forks. These findings are of the highest interest as at present we have little knowledge of this process. I will, therefore, study the mechanism and the consequences of this defect. I will also investigate the effects of blocking sumoylation on replisome formation, regulation and disassembly.
Once novel ubiquitylated and sumoylated proteins have been identified and basic mechanistic studies have been performed using Xenopus egg extracts I will select a few most interesting substrates and study their modifications in human cell lines.
Planned Impact
My research will have an academic impact: enhancing the knowledge and worldwide academic advancement. I will also train future researchers.
The research I propose will lead to a greater understanding of the fundamental process of eukaryotic DNA replication. Problems arising during DNA replication are the main source of genomic instabilities that can lead to mutations and multiple diseases including cancer. The better understanding of this important process and the ways in which it can malfunction will therefore allow better understanding of the development of these diseases. Moreover, the process of DNA replication is also one of the main targets of anticancer therapies. Therefore advances in the understanding of the functioning of this process will help understanding how these therapies work and the design of more specific therapeutic agents. Altogether, in the long term my research will enhance the scientific knowledge leading to design of novel, better drugs and drugs combinations. The proposed research is therefore a basic research that will contribute to improved health and treatment outcomes in the long term.
Public beneficiaries of this research will include cancer patients, patients suffering from genetic disorders caused by mutations in replication factors, the NHS, cancer charities and biotechnology companies.
1. Cancer patients
Better treatments for cancer are of general interest among the public but especially among the cancer sufferers. To achieve this goal we need to better understand the ways cancer develops, the mechanisms by which current cancer therapies work to improve them and widen our knowledge of processes essential for cancer phenotype which can be targeted by new drugs. Many cancer patients are treated with radiotherapy and chemotherapy targeting DNA replication which although effective are dose-limited and cause many side effects. It is therefore of high interest to develop new targets affecting the essential for cancer development process of DNA replication, which will be as efficient as current drugs but much more specific for cancer cells.
2. Patients suffering from genetic disorders caused by mutations in replication factors (such as Rothmund-Thomson, Rapadilino syndromes caused by mutation in RecQ4)
As my research will increase our understanding of regulation of process of DNA replication it may increase our understanding of development of these disorders and may suggest novel ways to help patients with conditions caused by mutations in replication factors.
3. The NHS, cancer charities, pharmaceutical companies
All these organisations seek development of better, more specific anti-cancer drugs. The proposed research will be therefore of benefit for all of them.
Finally, through public engagement I would like to contribute to increase of public awareness and understanding of science and share my enthusiasm towards science with public.
The research I propose will lead to a greater understanding of the fundamental process of eukaryotic DNA replication. Problems arising during DNA replication are the main source of genomic instabilities that can lead to mutations and multiple diseases including cancer. The better understanding of this important process and the ways in which it can malfunction will therefore allow better understanding of the development of these diseases. Moreover, the process of DNA replication is also one of the main targets of anticancer therapies. Therefore advances in the understanding of the functioning of this process will help understanding how these therapies work and the design of more specific therapeutic agents. Altogether, in the long term my research will enhance the scientific knowledge leading to design of novel, better drugs and drugs combinations. The proposed research is therefore a basic research that will contribute to improved health and treatment outcomes in the long term.
Public beneficiaries of this research will include cancer patients, patients suffering from genetic disorders caused by mutations in replication factors, the NHS, cancer charities and biotechnology companies.
1. Cancer patients
Better treatments for cancer are of general interest among the public but especially among the cancer sufferers. To achieve this goal we need to better understand the ways cancer develops, the mechanisms by which current cancer therapies work to improve them and widen our knowledge of processes essential for cancer phenotype which can be targeted by new drugs. Many cancer patients are treated with radiotherapy and chemotherapy targeting DNA replication which although effective are dose-limited and cause many side effects. It is therefore of high interest to develop new targets affecting the essential for cancer development process of DNA replication, which will be as efficient as current drugs but much more specific for cancer cells.
2. Patients suffering from genetic disorders caused by mutations in replication factors (such as Rothmund-Thomson, Rapadilino syndromes caused by mutation in RecQ4)
As my research will increase our understanding of regulation of process of DNA replication it may increase our understanding of development of these disorders and may suggest novel ways to help patients with conditions caused by mutations in replication factors.
3. The NHS, cancer charities, pharmaceutical companies
All these organisations seek development of better, more specific anti-cancer drugs. The proposed research will be therefore of benefit for all of them.
Finally, through public engagement I would like to contribute to increase of public awareness and understanding of science and share my enthusiasm towards science with public.
People |
ORCID iD |
Agnieszka Gambus (Principal Investigator / Fellow) |
Publications
Bailey R
(2015)
Termination of DNA replication forks: "Breaking up is hard to do".
in Nucleus (Austin, Tex.)
Colding-Christensen CS
(2023)
Profiling ubiquitin signalling with UBIMAX reveals DNA damage- and SCFß-Trcp1-dependent ubiquitylation of the actin-organizing protein Dbn1.
in Nature communications
D'Angiolella V
(2017)
Two paths to let the replisome go
in Cell Death & Differentiation
De Piccoli G
(2016)
The Initiation of DNA Replication in Eukaryotes
Gambus A
(2017)
Termination of Eukaryotic Replication Forks.
in Advances in experimental medicine and biology
Hadzhiev Y
(2019)
A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation.
in Nature communications
Moreno S
(2018)
Mitotic replisome disassembly in vertebrates
Moreno SP
(2020)
Mechanisms of eukaryotic replisome disassembly.
in Biochemical Society transactions
Moreno SP
(2015)
Regulation of Unperturbed DNA Replication by Ubiquitylation.
in Genes
Moreno SP
(2014)
Polyubiquitylation drives replisome disassembly at the termination of DNA replication.
in Science (New York, N.Y.)
Description | Birmingham Fellowship |
Amount | £96,000 (GBP) |
Funding ID | AV2 931 PhD Studentship |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 09/2013 |
End | 09/2016 |
Description | CRUK Birmingham Centre Development award |
Amount | £14,968 (GBP) |
Organisation | Cancer Research UK |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 01/2019 |
End | 08/2019 |
Description | College PhD studentship |
Amount | £80,000 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 09/2016 |
End | 09/2019 |
Description | ISSF |
Amount | £23,345 (GBP) |
Organisation | Wellcome Trust |
Department | Wellcome Trust Strategic Award |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 09/2017 |
End | 04/2018 |
Description | Intercalated BMedSci in Clinical Sciences Studentship |
Amount | £1,100 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 08/2013 |
End | 05/2014 |
Description | Lister Institute Research Award |
Amount | £200,000 (GBP) |
Organisation | Lister Institute of Preventive Medicine |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 08/2015 |
End | 08/2020 |
Description | Lister Institute Summer Studensthip |
Amount | £2,000 (GBP) |
Organisation | Lister Institute of Preventive Medicine |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 03/2017 |
End | 05/2017 |
Description | Lister Institute Summer Studentship |
Amount | £2,000 (GBP) |
Organisation | Lister Institute of Preventive Medicine |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 06/2016 |
End | 09/2016 |
Description | Lister Summer Studentship |
Amount | £2,000 (GBP) |
Organisation | Lister Institute of Preventive Medicine |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 06/2018 |
End | 09/2018 |
Description | MIBTP DTP PhD studentship |
Amount | £100,000 (GBP) |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2019 |
End | 09/2023 |
Description | MIBTP PhD studentship |
Amount | £90,000 (GBP) |
Organisation | Midlands Integrative Biosciences Training Partnership |
Sector | Academic/University |
Country | United Kingdom |
Start | 09/2017 |
End | 09/2021 |
Description | MRC Impact DTP PhD studensthip |
Amount | £100,000 (GBP) |
Organisation | Medical Research Council (MRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2021 |
End | 03/2025 |
Description | MRes Cancer Sciences Studentship |
Amount | £2,500 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 11/2013 |
End | 07/2014 |
Description | MRes Molecular and Cellular Biology |
Amount | £1,000 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 08/2014 |
End | 02/2015 |
Description | MSc Molecular Biotechnology |
Amount | £422 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 04/2014 |
End | 08/2014 |
Description | MSc Molecular Biotechnology studenntship |
Amount | £445 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 04/2017 |
End | 08/2017 |
Description | Marie Curie Sklodowska Fellowship for Dr Neville Gilhooly |
Amount | € 183,454 (EUR) |
Funding ID | 794962 |
Organisation | European Research Council (ERC) |
Sector | Public |
Country | Belgium |
Start | 01/2019 |
End | 12/2020 |
Description | Replisome unloading: mechanism and importance for cell biology |
Amount | £1,490,524 (GBP) |
Funding ID | 215510/Z/19/Z |
Organisation | Wellcome Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 08/2019 |
End | 08/2025 |
Description | Research Development Award - Travel award |
Amount | £2,000 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 05/2016 |
End | 06/2017 |
Description | Research development publication award |
Amount | £4,601 (GBP) |
Organisation | University of Birmingham |
Sector | Academic/University |
Country | United Kingdom |
Start | 11/2018 |
End | 01/2019 |
Description | TRAIP ubiquitin ligase driving replisome disassembly |
Amount | £521,819 (GBP) |
Funding ID | BB/T001860/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 12/2019 |
End | 08/2023 |
Description | summer studentship |
Amount | £2,000 (GBP) |
Organisation | Lister Institute of Preventive Medicine |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 05/2019 |
End | 08/2019 |
Description | Karim Cul2Lrr1 |
Organisation | University of Dundee |
Department | School of Nursing and Health Sciences |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We are exchanging information and reagents with Prof Karim Labib's group, University of Dundee. We are both working towards better understanding of DNA replication termination machanism and work in different model organisms. Due to that our work strengthens each other. |
Collaborator Contribution | Prof Labib's laboratory exchanged information and reagents with us. |
Impact | CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis. Sonneville R, Moreno SP, Knebel A, Johnson C, Hastie CJ, Gartner A, Gambus A, Labib K. Nat Cell Biol. 2017 May;19(5):468-479. doi: 10.1038/ncb3500. Epub 2017 Apr 3. |
Start Year | 2015 |
Description | collaboration with Massimo Lopez |
Organisation | University of Zurich |
Country | Switzerland |
Sector | Academic/University |
PI Contribution | we are collaborating with lab of Massimo Lopes to use their electron microscopy technique |
Collaborator Contribution | providing electron microscopy analysis |
Impact | not yet |
Start Year | 2017 |
Description | collaboration with mark Wiglesworth |
Organisation | AstraZeneca |
Country | United Kingdom |
Sector | Private |
PI Contribution | We are both supervising a PhD student funded by BBSRC MIBTP PhD programme. I am the main supervisor and the student will carry on the project in my laboratory. |
Collaborator Contribution | We are both supervising a PhD student funded by BBSRC MIBTP PhD programme. Mark Wigglesworth is a second supervisor and AZ will fund a placement for the student later during the project. |
Impact | not yet |
Start Year | 2017 |
Description | "Science is a girl thing" |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | I led "Science is a Girl Thing" Facebook question time organised by European Commission to encourage girls from anywhere in the world to become scientists. |
Year(s) Of Engagement Activity | 2014 |
Description | "Science knows no borders" blog |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | I wrote a blog entry on World Cancer Day about international nature of science, which was published at Foreign Office website |
Year(s) Of Engagement Activity | 2017 |
URL | https://blogs.fco.gov.uk/fcoeditorial/2017/02/03/science-knows-no-borders/ |
Description | "meet the scientist" |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | I have taken part in "meet the scents" event organised at Thinktank in Birmingham to share science with children |
Year(s) Of Engagement Activity | 2012 |
Description | CSHL meeting presentation |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I presented our work at Cold Spring Harbour DNA Replication meeting, September 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | Family in Academia workshop |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I organised workshop to highlight that family and career in academia can be successfully combined |
Year(s) Of Engagement Activity | 2019 |
Description | Gave a talk at Cell Cycle Club, London, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | I talked about our research at the above club |
Year(s) Of Engagement Activity | 2013 |
Description | Gave a talk at EMBO Ubiquitin and Ubiquitin-like Proteins: from structure to function, Riva del Garda, Italy |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Gave a talk about my work at the above international conference |
Year(s) Of Engagement Activity | 2013 |
Description | Gave a talk at Genome Stability Network meeting, Cambridge, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Gave a talk at a UK based meeting about my work |
Year(s) Of Engagement Activity | 2014 |
Description | Gave a talk at International symposium "Cell proliferation and genome integrity", Santander, Spain |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | gave a talk at an international conference |
Year(s) Of Engagement Activity | 2014 |
Description | Girls in STEM workshop |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | I run a workshop about academic careers for secondary school girls interested in career in STEM |
Year(s) Of Engagement Activity | 2014 |
Description | Invited seminar at BRIC, Copenhagen |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to talk about my research at BRIC Institute, Copenhagen, Denmark |
Year(s) Of Engagement Activity | 2018 |
Description | Invited seminar at Dunn School of Pathology, Oxford |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to talk about my work at Oxford. |
Year(s) Of Engagement Activity | 2014 |
Description | Invited seminar at Genome Stability Centre, Sussex |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I gave invited seminar about my research |
Year(s) Of Engagement Activity | 2020 |
Description | Invited seminar at Southampton University |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to give a talk about my work at the University of Southampton |
Year(s) Of Engagement Activity | 2016 |
Description | Invited speaker at the 16th International Xenopus Conference, Crete, Greece |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to talk about my work at the 16th International Xenopus Conference, Crete, Greece |
Year(s) Of Engagement Activity | 2016 |
Description | Keynote speaker at London Cell Cycle Club |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | I was the keynote speaker about my research |
Year(s) Of Engagement Activity | 2020 |
Description | Presentation at the Lister Institute Fellows meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | I talked about my research and discoveries upon being awarded the Lister Research Prize. |
Year(s) Of Engagement Activity | 2015 |
Description | Presentation at the NCRI conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | I described my research at the National Cancer Research conference (NCRI meeting) |
Year(s) Of Engagement Activity | 2015 |
Description | Sara's talk at Genome Stability Network meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | My PhD student Sara presented her work at the Genome Stability Network meeting |
Year(s) Of Engagement Activity | 2017 |
Description | Sara's talk in Madrid |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | My PhD student Sara was invited to talk about our work at the Research Institute in Madrid |
Year(s) Of Engagement Activity | 2017 |
Description | School open day |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | I organised "how frogs can help cancer" station during the open day |
Year(s) Of Engagement Activity | 2013 |
Description | Seminar at University of Sheffield |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | I was invited to talk about our work at University of Sheffield |
Year(s) Of Engagement Activity | 2021 |
Description | Taking part in Birmingham University open days |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Every year I help with the university open days actively interacting with public during activities explaining cancer research. |
Year(s) Of Engagement Activity | 2011,2012,2013,2014 |
Description | UK DNA Replication 2022 meeting |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | I was a main organiser of the international conference: UK DNA replication 2022, that happened in Birmingham in September 2022 |
Year(s) Of Engagement Activity | 2022 |
Description | Workshop at NCRI conference |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | I was invited by BACR to deliver a workshop at NCRI conference, Liverpool in November 2017. |
Year(s) Of Engagement Activity | 2017 |
Description | a presentation at Cell Cycle Club, London |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | A postdoctoral scientist from my lab had given a presentation at London Cell Cycle Club about our research |
Year(s) Of Engagement Activity | 2018 |
Description | hosting CRUK fundraisers for lab tours |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Supporters |
Results and Impact | we host CRUK fundraisers etc during lab tours to explain our science |
Year(s) Of Engagement Activity | 2020 |
Description | invited seminar at Barts |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to talk about our work at the Barts Institute, London |
Year(s) Of Engagement Activity | 2018 |
Description | invited seminar at Clare Hall Laboratories, CRUK London |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to talk about my work at Clare Hall Laboratories, CRUK London |
Year(s) Of Engagement Activity | 2016 |
Description | invited seminar at Oxford Institute for Radiation Biology, Oxford |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | invited seminar at Oxford Institute for Radiation Biology, Oxford discussed our work in depth |
Year(s) Of Engagement Activity | 2018 |
Description | invited seminar at Sussex Genome Damage and Stability Centre, University of Sussex |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to talk about my work at Sussex Genome Damage and Stability Centre, University of Sussex |
Year(s) Of Engagement Activity | 2016 |
Description | invited seminar at University of Edinburgh |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | I have been invited to give a talk about our work at University of Edinburgh |
Year(s) Of Engagement Activity | 2018 |
Description | invited seminar at University of Liverpool, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | Invited seminar at university of Liverpool Talking to scientists |
Year(s) Of Engagement Activity | 2019 |
Description | invited seminar at the MRC Clinical Sciences Centre, Imperial College London |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to give a seminar about my work |
Year(s) Of Engagement Activity | 2015 |
Description | invited speaker at BSCB/BSDB Spring Meeting 2019, Warwick, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | I gave an invited presentation during large BSCB/BSDB joined Spring Meeting in Warwick, UK explaining my science and its importance |
Year(s) Of Engagement Activity | 2019 |
Description | invited speaker at Birmingham Centre for Genome Biology launch meeting, Birmingham |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to talk about my work at the Birmingham Centre for Genome Biology launch meeting in Birmingham |
Year(s) Of Engagement Activity | 2016 |
Description | poster at Chromatin, Replication and Chromosomal Stability Meeting in Copenhagen, Denmark |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I presented my work at Chromatin, Replication and Chromosomal Stability Meeting in Copenhagen, Denmark |
Year(s) Of Engagement Activity | 2016 |
Description | presentation at ABTA award ceremony |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Dr Sara Priego Moreno gave a talk about her work at ABTA awards ceremony in London describing her PhD work |
Year(s) Of Engagement Activity | 2018 |
Description | presentation at EMBO Symposium, DNA replication: From Basic Biology to Disease, Heidelberg, Germany |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Oral presentation at EMBO Symposium, DNA replication: From Basic Biology to Disease, Heidelberg, Germany presented our recent work |
Year(s) Of Engagement Activity | 2018 |
Description | presentation at Genome Stability Network meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Postdoctoral scientist from my lab Dr Becky Jones gave a talk at GSN meeting, Cambridge |
Year(s) Of Engagement Activity | 2019 |
Description | presentation at the College of medical and dental sciences |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | I talked about my work in my College - to enthuse students and postdocs. |
Year(s) Of Engagement Activity | 2016 |
Description | presentation at the EMBO Ubiquitin and UBLs meeting in Buenos Aires, Argentina |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Presented my research at the international conference |
Year(s) Of Engagement Activity | 2014 |
Description | presented a poster at ICGEB DNA replication meeting in Trieste, Italy |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | presented my work at ICGEB DNA replication meeting in Trieste, Italy |
Year(s) Of Engagement Activity | 2016 |
Description | pretty muddy |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | I've taken part but also spoke about my work at one of the CRUK Pretty Muddy runs |
Year(s) Of Engagement Activity | 2016 |
Description | seminar at Institute for Cancer and Genomic Sciences |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | I gave seminar about our work in the institute |
Year(s) Of Engagement Activity | 2020 |
Description | seminar at University of Leeds |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | I gave a seminar explaining our work to students and staff at University of Leeds |
Year(s) Of Engagement Activity | 2021 |
Description | talk at "Views into nuclear function" meeting, Patras, Greece |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I gave a talk about my work at the international conference in Patras, Greece |
Year(s) Of Engagement Activity | 2014 |
Description | talk at Cold Spring Harbour DNA Replication meeting, USA |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I gave a talk at main international DNA Replication meeting about my research |
Year(s) Of Engagement Activity | 2019 |
Description | talk at EMBO workshop, DNA replication, chromosome segregation and fate decision, Kyllini, Greece |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | talk at EMBO workshop, DNA replication, chromosome segregation and fate decision, Kyllini, Greece presented our recent work |
Year(s) Of Engagement Activity | 2018 |
Description | talk at the Experimental Biology Meeting, San Diego, USA, invited by The American Society for Biochemistry and Molecular Biology (ASBMB) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited by by The American Society for Biochemistry and Molecular Biology (ASBMB) to talk about my work at talk at the Experimental Biology Meeting, San Diego, USA, |
Year(s) Of Engagement Activity | 2016 |
Description | tweet my day |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | I tweeted my day on International Women Day 2017 to raise awarness of what a scintist and a mother job looks like |
Year(s) Of Engagement Activity | 2017 |