Super Resolution Imaging for Cell Biology and Neuroscience at UCL

Lead Research Organisation: University College London
Department Name: MRC Laboratory of Molecular Cell Biology

Abstract

Microscopy has been one of the principle tools for discovery-based research at the cell and molecular level, allowing insights to the organisation and dynamics of cells, as well as the faults associated with infection and disease. Over the last 25 years increasingly sophisticated microscopes, together with new experimental tools such as fluorescent protein tags, improved lasers and cameras, and developments in live cell imaging, have led to rapid advances in our understanding of cell organisation and function. Nevertheless, imaging at high resolution is limited by the properties of light to ~250 nm, meaning that information at the level of molecules and molecular complexes cannot be obtained using conventional light microscopes. Thus, high-resolution studies have been restricted to electron microscopy (EM), which can only be used on fixed and highly processed samples, and has limited capacity to identify specific molecular components.

In recent years several novel optical and computational approaches have been developed to break the diffraction-limit of conventional light microscopy, with the potential to obtain molecular/structural information at significantly higher resolution (approx. 2-20 fold improvement). By combining multiple fluorescently-labelled probes, within the same sample, together with live cell imaging, these so-called super-resolution nanoscopy techniques are able to bridge the current technologies of diffraction-limited microscopy and EM to generate new detailed molecular and mechanistic insights into the cellular and molecular processes that underlie normal cell function and disease.

Super-resolution imaging (SRI) instruments are now becoming commercially available allowing cellular biomedical researchers to begin to apply these exciting new technologies to a multitude of questions in cell biology, neurobiology, infectious disease and many other fields of biomedical research. Development is still at an early stage and there remain many opportunities for progress, particularly in live cell imaging, probe development, software development, analytical tools, etc. However, SRI instruments and technical developers are needed now to work in close collaboration with biomedical researchers to establish the technology and push instrument and application development.

This application will link MRC-funded scientists in the Cell Biology Unit/Laboratory for Molecular Cell Biology (CBU/LMCB) and Prion Unit (MRC PU), as well as the MRC/UCL Centre in Molecular Medical Virology (MRC CMMV), with members of UCL's Faculties of Life Sciences, Medicine and Brain Sciences, the London Centre for Nanotechnology (LCN), UCL physicists and chemists, computer scientists, image-based bio-informaticians and the National Physics Laboratory Teddington (NPL), to establish and develop platforms for SRI.

The facility will be centred on commercial off-the-shelf SRI instruments that will provide a benchmark against which UCL and NPL members of the programme will develop new systems and instruments optimised for biomedical research. The facility will also provide capacity-building opportunities for students and postdoctoral fellows to train/work at the interface of biology, imaging and instrument development. Together this presents a timely window of opportunity in which to build on UCL's excellent imaging facilities and world leading biomedical research community to create a cutting edge, interactive SRI research and development programme for application to biomedical research.

Technical Summary

We aim to develop a super-resolution fluorescence imaging (SRFI) facility that will link MRC scientists in the Cell Biology and Prion Units, as well as the MRC/UCL Centre in Molecular Medical Virology, with members of UCL's Faculties of Life Sciences, Medicine and Brain Sciences, the London Centre for Nanotechnology, UCL physicists and chemists, computer scientists, image-based bio-informaticians and the National Physics Laboratory, Teddington (NPL).

The facility will house commercial SRFI instruments for use on a range of projects with particular emphasis on cell biology, neurobiology and infectious disease. These instruments will provide state of the art bench-marks against which UCL and NPL researchers will develop new instruments, optimised for biomedical research.

We are requesting funds to:-
1. Purchase a Zeiss Elyra PS1 that will provide PALM/STORM and SIM capability, providing a multi-user platform through which UCL (Lowe, Kittler) and NPL (Knight) PALM/STORM systems and the NPL SIM system can be developed, compared and optimised. We will use UCL funds to buy a second commercial instrument to offer a broad range of imaging options.

2. Support two postdoctoral RAs to train/work at the interface of biology, imaging and instrument development under the guidance of the facility's Management Board. There will be additional capacity-building opportunities linked to the facility for UCL students and postdoctoral fellows.

3. Support development, including cloning the NPL and Lowe lab. instruments for use by UCL researchers. Significantly, the software for these instruments will be open source, allowing better assessment and control over data processing/analysis.

The facility will enable research into fundamental questions in biomedical research, providing new insights to disease mechanisms and opportunities for therapeutic development. The facility and its Management Board will also provide a focus to coordinate imaging developments at UCL.

Planned Impact

Dissemination of findings to the scientific community.
The main route for disseminating our results will be through publication in international peer-reviewed scientific journals. Most of the project leaders in the application are leaders in their fields and are frequently invited to present their work at international meetings. In addition, user groups that meet regularly at UCL, such as the annual LMCB hosted UK Membrane Trafficking meeting, with 300 plus participants, offer ideal forums to update the national community on advances and opportunities. Thus, the results emerging from our work will have impact far beyond our immediate circle of specialists.

Dissemination of findings to the end user.
Our results and their implications will be presented to the broader scientific community and the lay public through multiple routes. We will establish a web site for the Advanced BioImaging Partnership (ABP) through which information on resources, applications, etc. will be made available. New results and tools, including open source software developed by the programme, will also be available through this site. When appropriate, information will be released through the UCL or MRC Press Offices.
The LMCB operates a schools engagement programme to encourage younger students to develop their interest in cell biology. Students will be able to visit the facility, see the instruments in use and view new findings. The facility will also be open to visitors on LMCB open days.

Establish collaborations within the scientific community and with industry.
The ABP will link biomedical researchers with instrument developers to provide a more integrated approach to SRFI. It is our intention to hold regular (at 3-6 month intervals) user meetings for results to be presented and discussed and problems resolved. We aim to create a long lasting forum through which these exciting new technologies are effectively applied and developed within UCL and extended to other similar groups across the UK. We also advocate at least annual meetings between all the advanced imaging hubs funded by the MRC, as well as links between their web sites to allow users to obtain a clear view of what is available at different locations.
The development work to be undertaken may lead to opportunities for commercialisation. Such opportunities will be taken forward through UCL Business or MRC-T.
The results from our work will be of broad interest. These new insights to the molecular organisation and function of cells will reveal new opportunities for therapy and drug development. In this respect the ABP will complement the recently established CBU/LMCB high throughput, high content screening platform.

Training of people.
The ABP will have a strong training element. UCL hosts a number of Postgraduate Training Programmes including the CBU/LMCB four year graduate programme, the new BBSRC London Interdisciplinary Biosciences PhD Consortium and CoMPLEX. CoMPLEX, provides a training programme that links physics/maths and engineering with biology. The new facility will provide opportunities for CoMPLEX students to gain insight into advanced imaging methodologies with the view that they may opt to work in this area for rotations and/or thesis projects, providing further opportunities for capacity building and development. The programme will also support postdoctoral training.

Fostering a collaborative environment.
Biomedical Imaging at UCL is well developed and focussed on the Centre for Advanced Biomedical Imaging. However, imaging at the cell and molecular level has not been as well integrated. The ABP will create a opportunity to focus this latter community by providing state of the art imaging platforms, along with seed funding and meeting forums, to encourage interactions. We believe that ABP will lead to cross disciplinary collaborations, that will make significant new insights to the molecular mechanisms of normal and abnormal cell function.

Publications

10 25 50
 
Description Competitive interactions in heterogenous cancer cell populations; a multimodal imaging, machine learning and computational modelling approach
Amount £94,623 (GBP)
Funding ID EP/N509577/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 09/2021
 
Description EPSRC First Grant to Isabel Llorente-Garcia Project title: Direct probing of molecular interactions relevant to virus entry via force spectroscopy with optical tweezers in live cells
Amount £112,000 (GBP)
Funding ID EP/P020747/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 06/2017 
End 05/2019
 
Description Impact Acceleration Account
Amount £10,000 (GBP)
Funding ID BB/IAA/UCL/15 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2015 
End 07/2016
 
Description Integrated microscopy approach to protein assembly on and in membranes
Amount £458,449 (GBP)
Funding ID BB/N015487/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2016 
End 10/2019
 
Description Micro-optics and photosynthetic light-trapping in cyanobacteria
Amount £44,685 (GBP)
Funding ID BB/P000568/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2016 
End 11/2019
 
Description Micro-optics and photosynthetic light-trapping in cyanobacteria
Amount £344,738 (GBP)
Funding ID BB/P001807/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2017 
End 12/2020
 
Description Multi-user equipment grant
Amount £85,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2013 
End 05/2015
 
Description Sir Henry Dale Fellowship
Amount £900,000 (GBP)
Funding ID 107653/Z/15/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2016 
End 12/2021
 
Description UCL Matching funds award to Isabel Llorente-Garcia - Project title: A fast fluorescence and photonic force microscope with nanometre and femtoNewton resolution - Isabel Llorente-Garcia
Amount £40,000 (GBP)
Organisation University College London 
Sector Academic/University
Country United Kingdom
Start 06/2014 
End 12/2017
 
Description Andor Technology 
Organisation Andor Technology
Country United Kingdom 
Sector Private 
PI Contribution R&D partnership to implement analytical technologies developed into commercial turnkey systems.
Collaborator Contribution R&D partnership to implement analytical technologies developed into commercial turnkey systems.
Impact This collaboration has led to the translation of UCL developed technology into turn-key commercial hardware for biomedical research. As part of this collaboration Andor is bringing experimental kit and software to UCL that local researchers in biology and physics can use in their research.
Start Year 2017
 
Description Andor Technology 
Organisation Andor Technology
Country United Kingdom 
Sector Private 
PI Contribution Developed the Super-Resolution Radial Fluctuations method which was used by Andor Technology to establish the worlds first Super-Resolution Cameras for fluorescence microscopy, which are now commercially available.
Collaborator Contribution Andor Technology has contributed with known-how and research financial support.
Impact This R&D collaboration has led to the development of a new generation of cameras for microscopy and spinning disk microscopes, these are now available commercially. This collaboration entails expertise in optical physics, cell biology and electrical engineering.
Start Year 2017
 
Description Darren Tomlinson, University of Leeds 
Organisation University of Leeds
Country United Kingdom 
Sector Academic/University 
PI Contribution Assays for EGFR/Grb2 signaling and binding studies
Collaborator Contribution Provision of non-antibody binding protein inhibitors of Grb2
Impact None to date
Start Year 2013
 
Description Dr Darren Tomlinson 
Organisation University of Leeds
Department School of Chemistry Leeds
Country United Kingdom 
Sector Academic/University 
PI Contribution Validation of the small binders against CD4 receptors.
Collaborator Contribution Phage-display screening of small binders agains CD4 receptors.
Impact This collaboration is multi-disciplinary, involving the expertise of the Henriques lab (cell biology biophysics, mathematics and synthetic photobiochemistry) and the Tomlinson lab (biochemistry and cell biology).
Start Year 2015
 
Description Dylan Owen - super-resolution studies on T-cell signalling 
Organisation King's College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of optical and analytical technologies to enable quantitative super-resolution studies on T-cell signalling.
Collaborator Contribution Joint development of the new super-resolution method SRRF and cell signalling studies.
Impact Joint publication: Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations N Gustafsson, S Culley, G Ashdown, DM Owen, PM Pereira, R Henriques Nature Communications 7
Start Year 2014
 
Description Eva-Maria Frickel - super-resolution studies on host-pathogen interactions 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of optical and analytical technologies to enable quantitative super-resolution studies on host-pathogen interactions.
Collaborator Contribution Studies on Toxoplasma gondii host-pathogen interactions.
Impact Joint publication: K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFN?-Stimulated Human Cells B Clough, JD Wright, PM Pereira, EM Hirst, AC Johnston, R Henriques, ... PLoS Pathogens 12 (11), e1006027
Start Year 2016
 
Description Helena Soares, CEDOC, Portugal - super-resolution studies on T-cell signalling 
Organisation New University of Lisbon
Department Chronic Diseases Research Centre (CEDOC)
Country Portugal 
Sector Academic/University 
PI Contribution Development of optical and analytical technologies to enable quantitative super-resolution studies on T-cell signalling.
Collaborator Contribution Joint studies on cell signalling.
Impact Joint publication: HIV-1 Nef Impairs the Formation of Calcium Membrane Territories Controlling the Signaling Nanoarchitecture at the Immunological Synapse JG Silva, NP Martins, R Henriques, H Soares The Journal of Immunology 197 (10), 4042-4052
Start Year 2013
 
Description Intelligent Imaging Innovations 
Organisation Intelligent Imaging Innovations Ltd
Country United Kingdom 
Sector Private 
PI Contribution R&D partnership to implement analytical technologies developed into commercial turnkey systems.
Collaborator Contribution R&D partnership to implement analytical technologies developed into commercial turnkey systems.
Impact This collaboration has led to the establishment of UCL as the R&D reference site for 3i in the UK. As part of this collaboration 3i is bringing experimental kit and software to UCL that local researchers in biology and physics can use in their research.
Start Year 2016
 
Description Musa Mhlanga, Univ Cape Town, South Africa - super-resolution studies on NF-kappaB signalling 
Organisation Council of Scientific and Industrial Research (CSIR)
Country South Africa 
Sector Academic/University 
PI Contribution Development of optical and analytical technologies to enable quantitative super-resolution studies on NF-kappaB signalling.
Collaborator Contribution Joint studies on cell signalling.
Impact Joint publication: Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti J Scholefield, R Henriques, AF Savulescu, E Fontan, A Boucharlat, ... Nature Communications 7
Start Year 2011
 
Description Musa Mhlanga, Univ Cape Town, South Africa - super-resolution studies on NF-kappaB signalling 
Organisation Pasteur Institute, Paris
Department Chemogenomic and Biological Screening
Country France 
Sector Charity/Non Profit 
PI Contribution Development of optical and analytical technologies to enable quantitative super-resolution studies on NF-kappaB signalling.
Collaborator Contribution Joint studies on cell signalling.
Impact Joint publication: Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti J Scholefield, R Henriques, AF Savulescu, E Fontan, A Boucharlat, ... Nature Communications 7
Start Year 2011
 
Description Serge Mostowy - super-resolution studies on host-pathogen interactions 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of optical and analytical technologies to enable quantitative super-resolution studies on host-pathogen interactions.
Collaborator Contribution Studies on Shigella sonnei host-pathogen interactions.
Impact Joint publication: Mitochondria mediate septin cage assembly to promote autophagy of Shigella A Sirianni, S Krokowski, D Lobato-Márquez, S Buranyi, J Pfanzelter, ... EMBO reports, e201541832
Start Year 2016
 
Title NanoJ 
Description In recent years, our team has built an open-source image analysis framework for super-resolution microscopy designed to combine high performance and ease of use. We named it NanoJ - a reference to the popular ImageJ software it was developed for. In this paper, we highlight the current capabilities of NanoJ for several essential processing steps: spatio-temporal alignment of raw data (NanoJ-Core), super-resolution image reconstruction (NanoJ-SRRF), image quality assessment (NanoJ-SQUIRREL), structural modelling (NanoJ-VirusMapper) and control of the sample environment (NanoJ-Fluidics). 
Type Of Technology Software 
Year Produced 2018 
Open Source License? Yes  
Impact NanoJ provides the technological basis to a series of high-performance analytical algorithm for super-resolution microscopy data analysis 
URL https://iopscience.iop.org/article/10.1088/1361-6463/ab0261/meta
 
Title NanoJ - GNU GPL (PI - 2018): Laine et al., bioR iv, 2018 - High-performance open-source super-resolution microscopy toolbox, capable of GPU acceleration. 
Description Super-resolution microscopy has become essential for the study of nanoscale biological processes. This type of imaging often requires the use of specialised image analysis tools to process a large volume of recorded data and extract quantitative information. In recent years, our team has built an open-source image analysis framework for super-resolution microscopy designed to combine high performance and ease of use. We named it NanoJ - a reference to the popular ImageJ software it was developed for. In this paper, we highlight the current capabilities of NanoJ for several essential processing steps: spatio-temporal alignment of raw data (NanoJ-Core), super-resolution image reconstruction (NanoJ-SRRF), image quality assessment (NanoJ-SQUIRREL), structural modelling (NanoJ-VirusMapper) and control of the sample environment (NanoJ-Fluidics). We expect to expand NanoJ in the future through the development of new tools designed to improve quantitative data analysis and measure the reliability of fluorescent microscopy studies. 
Type Of Technology Software 
Year Produced 2018 
Open Source License? Yes  
Impact https://iop.altmetric.com/details/54658315 
 
Title NanoJ-Fluidics 
Description Fluorescence microscopy can reveal all aspects of cellular mechanisms, from molecular details to dynamics, thanks to approaches such as super-resolution and live-cell imaging. Each of its modalities requires specific sample preparation and imaging conditions to obtain high-quality, artefact-free images, ultimately providing complementary information. Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows involving multiple sample preparation steps. We present a robust fluidics approach to automate complex sequences of treatment, labelling and imaging of live and fixed cells. Our open-source NanoJ-Fluidics system is based on low-cost LEGO hardware controlled by ImageJ-based software and can be directly adapted to any microscope, providing easy-to-implement high-content, multimodal imaging with high reproducibility. 
Type Of Technology Software 
Year Produced 2018 
Open Source License? Yes  
Impact NanoJ-Fluidics is now the basis for commercial technology in development by Abbelight 
URL https://www.biorxiv.org/content/10.1101/320416v1
 
Title NanoJ-Fluidics - MIT License (PI - 2018): Almada et al., bioR iv, 2018 - Automating multimodal microscopy through inexpensive LEGO based syringe pumps. 
Description Fluorescence microscopy can reveal all aspects of cellular mechanisms, from molecular details to dynamics, thanks to approaches such as super-resolution and live-cell imaging. Each of its modalities requires specific sample preparation and imaging conditions to obtain high-quality, artefact-free images, ultimately providing complementary information. Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows involving multiple sample preparation steps. We present a robust fluidics approach to automate complex sequences of treatment, labelling and imaging of live and fixed cells. Our open-source NanoJ-Fluidics system is based on low-cost LEGO hardware controlled by ImageJ-based software and can be directly adapted to any microscope, providing easy-to-implement high-content, multimodal imaging with high reproducibility. We demonstrate its capacity to carry out complex sequences of experiments such as super-resolved live-to-fixed imaging to study actin dynamics; highly-multiplexed STORM and DNA-PAINT acquisitions of multiple targets; and event-driven fixation microscopy to study the role of adhesion contacts in mitosis. 
Type Of Technology Software 
Year Produced 2018 
Open Source License? Yes  
Impact Article has an altmetric score of 275 Picked up by 1 news outlets Blogged by 2 Tweeted by 408 Mentioned in 1 Google+ posts 63 readers on Mendeley 
URL https://www.biorxiv.org/content/10.1101/320416v1
 
Title NanoJ-SQUIRREL 
Description NanoJ-SQUIRREL is an analytical approach for quantifying image quality in super-resolution microscopy, provided as a GPU-enabled open-source ImageJ plugin. SQUIRREL requires two input images - a super-resolution image (or image stack) and the diffraction-limited equivalent of the same imaging volume. It then calculates an error-map, highlighting areas of the super-resolution image which exhibit poor agreement with the diffraction-limited image, and quality metrics for the super-resolution image. 
Type Of Technology Software 
Year Produced 2018 
Open Source License? Yes  
Impact NanoJ-SQUIRREL provides the technological basis to develop artificial intelligent self-driven microscopes. This is serving as a nucleator for collaborations with industry. 
URL https://bitbucket.org/rhenriqueslab/nanoj-squirrel/wiki/Home
 
Title NanoJ-SRRF (Super-Resolution Radial Fluctuations) 
Description SRRF (pronounced as surf) is as a novel open-source and high-performance analytical approach for Live-cell Super-Resolution Microscopy, provided as a fast GPU-enabled ImageJ plugin. SRRF is capable of extracting high-fidelity super-resolution information in modern microscopes (TIRF, widefield and confocals) using conventional fluorophores such as GFP. Compared to other methods, SRRF is capable of live-cell imaging over timescales ranging from minutes to hours, using sample illumination orders of magnitude lower than methods such as PALM, STORM or STED. 
Type Of Technology Software 
Year Produced 2016 
Open Source License? Yes  
Impact SRRF is a novel super-resolution analytical approach, since its publication it started being used by thousands of researchers (>50K uses since July 2016, as tracked by Google Analytics). There are now multiple laboratories publishing work that takes advantage of this software. 
URL https://bitbucket.org/rhenriqueslab/nanoj-srrf/wiki/Home
 
Title NanoJ-VirusMapper 
Description VirusMapper is a high-throughput, open-source ImageJ plugin for single-particle analysis in fluorescence microscopy, particularly super-resolution microscopy. It provides a platform for automatic statistical mapping of conserved multi-molecular structures, such as viral substructures or intact viruses. VirusMapper uses naive averaging to create models of the distribution of particular labelled species within larger structures. 
Type Of Technology Software 
Year Produced 2016 
Open Source License? Yes  
Impact This software is helping create the first multi-molecular structural maps of pox-like viruses and is currently being used by multiple laboratories to model the structure of cellular supramolecular complexes. 
URL https://bitbucket.org/rhenriqueslab/nanoj-virusmapper/wiki/Home
 
Description Host laboratory for In2ScienceUK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact We are a volunteer laboratory for the In2ScienceUK programme, an award winning initiative which empowers students from disadvantaged backgrounds to achieve their potential and progress to STEM and research careers through high quality work placements and careers guidance
Year(s) Of Engagement Activity 2016,2017,2018
URL http://in2scienceuk.org/
 
Description Isabel Garcia Llorente - Invited speaker at Next Generation Biophysics Symposium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact Symposium organised by MRC LMB Cambridge, UK, organised by MRC LMC, Imperial College London and Astra Zeneca to examine the application of cutting edge biophysical techniques in complex biological settings
Year(s) Of Engagement Activity 2018
 
Description Isabel Garcia Llorente - Talk for King's College London Women in Physics Group 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other audiences
Results and Impact 1 hour talk entitled Investigating molecular interactions relevant to virus entry into cells via optical tweezers force spectroscopy and light-sheet fluorescence microscopy.
Year(s) Of Engagement Activity 2018
 
Description London Super-Resolution Club Meetings 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I am one of the main organisers of the "London Super-Resolution Group" (LSR), a bi-monthly series of highly successful meetings bringing biologists, physicist, chemists and mathematicians together - showcasing their research with Super-Resolution Microscopy. This meeting series is designed to mix UK researchers from student to group leader level and high-profile speakers (such as the recently hosted Nobel Laureate Dr. Eric Betzig).
Year(s) Of Engagement Activity 2013,2014,2015,2016
URL https://www.ucl.ac.uk/super-resolution/events/UCLSuperResolutionSymposium2015/Home
 
Description Super-Resolution microscopy in Infection and Immunity Symposium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The "Super-Resolution microscopy in Infection and Immunity Symposium" was an international meeting bringing together multi-disciplinary researchers interested in the use of next-generation super-resolution imaging approaches to study immunology and host-pathogen interactions. It was jointly organised between Instituto Gulbenkian (Portugal), UCL (UK) and ITQB (Portugal).
Year(s) Of Engagement Activity 2015
URL http://www.igc.gulbenkian.pt/pages/evento.php/A=35701___collection=events
 
Description UCL Super-Resolution Symposium 2015 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The "UCL Super-Resolution Symposium" was an international meeting on super-resolution microscopy with free registration. It has showcased cutting-edge multi-disciplinary research in the field through seminal talks, including one by the Nobel Prize Laureate Dr. Eric Betzig. This symposium also celebrated the inauguration of the super-resolution facility at UCL nucleated by an MRC Next Generation Optical Microscopy award.
Year(s) Of Engagement Activity 2015
URL https://www.ucl.ac.uk/super-resolution/events/UCLSuperResolutionSymposium2015/Home