Using structural and chemical biology to understand the roles and mechanisms of CDKs: generating hypotheses for drug discovery

Lead Research Organisation: Newcastle University
Department Name: Translational and Clinical Res Institute

Abstract

The behaviour of a cell depends on the genes it expresses and on its commitment to either a dormant or a proliferating state. The cyclin-dependent kinases (CDKs) bind to members of the cyclin protein family to form complexes that regulate both the expression of genes and cell proliferation. Transcription describes the process by which a gene sequence is converted into mRNA. Transcriptional CDKs regulate this process, mostly by controlling the activity of an enzyme that synthesizes the mRNA. Transcriptional CDKs also regulate RNA processing events. CDKs that control the cell cycle are activated in response to growth promoting signals and control the timing of the duplication of the genome and its subsequent segregation to generate two identical copies when the cell divides. Just as CDK-cyclins are important in normal cells, so they can also contribute to the development of disease when they do not function properly. The first part of our research programme is to advance understanding of the structures and functions of CDK-containing complexes. We have selected CDK-cyclins to study based on their roles in the development of specific cancers. We aim to find proteins that these CDK-cyclins bind to, discover their 3D structures, and characterise how CDK activity is regulated within these structures. We can then study how these CDK complexes contribute to the development of disease when they do not function correctly. The techniques of X-ray crystallography and, in recent years, cryo-electron microscopy allow us to image protein complexes in atomic detail and we will use both methods. We use bacteria, insect or cultured mammalian cells to generate the proteins for study by crystallography or cryoEM. The proteins can also be used in functional assays to determine, for example, how tightly they bind to one another, and what effect mutations have on their properties.

Our second aim is to exploit insight into CDK-cyclin complexes to generate ideas for how they may better be targeted by inhibitors. Historically the development of CDK inhibitors has targeted the CDK ATP-binding site. These inhibitors outcompete ATP, a cofactor that CDKs normally use, and thereby block the CDK's catalytic activity. This approach cannot distinguish the different activities of their CDK target, which may depend on the complexes in which they are found. Consequently, ATP-competitive inhibitors can have unwanted effects that limit their use as drugs. The use of CDK inhibitors in cancer therapy has been pioneered by a first generation of mixed CDK4/6 inhibitors, but tumours are already developing resistance to these inhibitors. To improve the safety and increase the robustness of clinical response to CDK inhibitors, our programme will identify opportunities to inhibit CDKs that do not target the ATP-binding site; (i) by first identifying hotspots on the CDKs and cyclins through which they interact with other protein partners and then developing inhibitors that block those hotspots (so-called protein-protein interaction inhibitors or PPIs); and (ii) by exploiting our understanding of the structural changes that accompany CDK activation to design "allosteric inhibitors" that prevent CDK activation. We will use a set of small molecules called "FragLites" that are designed to find potential interaction hotspots on a protein through an X-ray crystallographic screen. We will also identify cyclic peptides that bind selectively and with high affinity to our CDK-cyclin targets. We will develop and characterise both our FragLite and cyclic peptides to identify more potent PPIs and allosteric inhibitors.

Overall, our programme will allow us to address a barrier between basic science and validated projects that drug discovery groups can adopt. The approaches will deliver both novel biological insight, and actionable approaches to novel ways of inhibiting CDKs for drug design.

Technical Summary

This programme will exploit our collaborations and strengths with CDK reagents, biochemical/biophysical assays, structural biology and small molecule and peptide chemistry to dissect the mechanisms by which CDKs regulate the cell cycle and transcription.

Our first aim is to advance understanding of the activity of CDK-containing complexes. To this end we will:
(i) Continue crystallographic and cryo-EM studies of CDK9-cyclin T-containing and p27KIP1-CDK2-cyclin A-containing complexes
(ii) Initiate crystallization trials of CDK11 and CDK11-cyclin L/D3 complexes.
(iii) Use structural and biophysical techniques to define the cyclin K-SETD1A interaction.
(iv) Carry out crystallographic fragment ("FragLite") screens against (i) CDK4/6-cyclin D1/3 complexes and (ii) CDK2-cyclin E. We will assess the functional significance of hotspots identified in this way by directed mutagenesis followed by cell free (SPR, ITC, HTRF) and cell-based assays for known protein partners. We will use affinity purification mass spectrometry to identify new interactors and initiate structural studies for tractable and biologically significant protein interactions identified.

Our second aim is to exploit structural insight into CDK-cyclin complexes to develop hypotheses for allosteric and PPI approaches to drug discovery. To this end we will:
(i) Exploit our FragLite map of cyclin T to generate molecular and chemical tools to dissect the impact of specific cyclin T interactions on P-TEFb-dependent transcriptional programs. We will compare the effects of this approach to that of conventional CDK9 inhibitors and use this workflow to guide studies to follow up our FragLite screen of cyclin K.
(ii) Carry out mRNA-display screens to identify potent CDK11 and cyclin L, CDK4, CDK6 and CDK-cyclin D3, and cyclin K-specific cyclic peptides. This work will enable functional studies to identify and characterise CDK and cyclin protein binding sites and roles.

Publications

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