MICA: Liverpool Imaging Partnership: Molecular physiology and drug response

Lead Research Organisation: University of Liverpool
Department Name: Institute of Integrative Biology

Abstract

In multicellular organisms, cells are exposed to molecular signals such as inflammatory molecules and stress signals. Cells have to interpret these signals to adapt and respond appropriately. To understand the molecular mechanisms leading to a particular response (e.g. cell death, cell growth, cell migration, etc), biologists have to measure the levels, localisation and modifications of proteins, and the subsequent regulation of specific genes. There is an increasing recognition that in a population of cells, each cell is different and therefore measuring the average behaviour can lead to erroneous conclusions. Live cell imaging coupled with the use of fluorescent and luminescent labels and reporters enables biologists to monitor dynamic events in individual cells in real time. Nanotechnology provides additional tools to visualise single molecules.

The aim of our project is to build on our existing imaging capabilities in the Centre for Cell Imaging and our world-leading expertise in the synthesis and imaging of nanoparticles to improve the equipment available with the most up-to-date microscopes. We will also develop new technologies, software and image analysis methods that will allow us to increase the resolution of the images and their interpretation (to better see the detailed structures inside the cells). We will make sure that the equipment is used to the best of its capabilities to answer important biological and biomedical questions. For this purpose, we will select the best projects, in Liverpool, the UK and beyond, and we will support users from the conception of their experiments to the analysis and interpretation of their results.

The CCI platform will therefore permit researchers from a broad range of research fields to tackle important 21st century challenges in biomedical research, by investigating in real-time cellular events at the molecular level. To illustrate the breadth of science that will be served by the platform, we briefly present below three exemplar projects that, among many others, will benefit of the improved facility.

UNDERSTANDING ACUTE PANCREATITIS ON THE NANOMETRE SCALE. Cells of the pancreas produce the enzymes that digest our food inside the intestine. In acute pancreatitis, instead of being secreted to the intestine, enzymes become active in the pancreas cells themselves and damage the pancreas leading to severe pain, hospitalisation and in some cases, death. The activation of these enzymes involves the formation of dynamic structures that are very difficult to study by conventional microscopy because they are smaller than the resolution of conventional microscopes. The new platform will uniquely allow us to study the response of those structures when pancreatic cells are exposed to molecules that may induce or prevent acute pancreatitis and ultimately develop novel approaches to prevent this from happening.

IMAGING THE SUBTLE ROLE OF OXIDATIVE STRESS IN AGEING; Oxidative stress leading to oxidative damage has been implicated in the processes underlying ageing for over 50 years, but recent data demonstrated that rather than gross changes in oxidative stress damage, it is the more subtle changes in regulation of reduction-oxidation reactions that play a fundamental role in ageing processes. The new platform will uniquely allow us to control and measure oxygen level and oxidative stress providing new insights into mechanisms of ageing.

PROBING THE MECHANISM FOR INTESTINAL ABSORPTION OF NANOMEDICINES; Insolubility of active pharmaceutical ingredients and the resulting lack of bioavailability is a major concern for the development of new drugs. Approaches based on nanotechnology have proven successful, but very little is known regarding the mechanistic basis. The present application will enable us to study differences in trans-intestinal passage and toxicity of nanomedicines compared to conventional dissolved drugs.

Technical Summary

The aim of Liverpool Imaging Partnership is to support excellent science with state-of-the-art live cell imaging. We will build on our expertise in long term live cell imaging and single molecule imaging using nanoparticles, to measure intracellular molecule dynamics in a wide range of living models including cells, tissues, small embryos and plants. To improve the sensitivity of detection and accurately measure protein-protein interactions, we will install a LSM 780 (Carl Zeiss) with Fluorescence Life time imaging (FLIM) component. The considerable increase in sensitivity of the LSM 780 allows it to be used in photon counting mode (FCS/FCCS). This will provide capabilities to measure intracellular sensors (e.g. oxygen nanosensors), protein-protein interactions, protein diffusion and complex formation in living samples. It will have direct application in several projects, such as elucidation of mechanisms of pancreatic damage, testing drug specificity. To increase our resolution capabilities and correlate intracellular dynamics with ultrastructural changes, we will develop two new techniques: 1) super-resolution optical fluctuation microscopy (SOFI), which requires a limited investment in comparison with other super resolution techniques and logically builds on our expertise in quantum dot development; 2) photothermal-correlative light microscopy (p-CLEM) where the gold standard of EM probes becomes also an excellent optical probe. This will constitute a platform with high sensitivity state-of-the-art confocal microscopes coupled with photothermal imaging of nanoparticles that is dedicated for long term imaging and imaging in physiologic settings including oxygen controlled environment. Altogether, this will provide unique measurements in living cells supporting research in areas such as inflammation, ageing, cancer, drug safety, nanomedicine, plant biology, stem cell research etc. in a platform that will support users from experimental design to image analysis.

Planned Impact

1. The Partnership will first impact on the scientific community by providing new tools for cell imaging. This will primarily impact on the research developed at the University of Liverpool, but also on other UK and overseas Universities (see letters of support). The strengthening of the CCI will provide for a step change in the ability to measure intracellular events at the molecular level, which will synergise with the existing research base. This will ultimately have impact on society by providing new knowledge, some of which will be critical to translational projects. Important beneficiaries will, therefore, be academics and clinicians. Examples include the MRC-funded Centres for Drug Safety and Integrated Research into Musculoskeletal Ageing and NHS stakeholders, such as the NIHR-funded Pancreas Biomedical Research Unit, Phase I/IV capability and future (MHRA accreditation due October 2012) Phase I trials unit.
2. The Partnership will also have economic impact, since IP will be actively protected and routes to exploitation pursued. For example, DF has patented novel stabilisation and functionalisation of quantum dots and nanoparticles for use in sensors and diagnostics; SR is co-founder of two spin out companies, IOTA NanoSolutions Limited and Hydra Polymers Limited. Our contribution to the development of SOFI, which is a versatile approach to super-resolution, will support its adoption and implementation by a large body of users and, will have direct (low cost super-resolution, development of new software in collaboration with Carl Zeiss through a case studentship) and indirect (via the discoveries enabled by SOFI) economic impact. The Liverpool Imaging Partnership will facilitate the training of the next generation of scientists in a multidisciplinary and vibrant environment to ensure the excellence of the UK science base.
Way to Impact: To achieve this economic impact, the University has a dedicated team, Business Gateway (BG), which aims to facilitate links between the university and companies and other external organisations. At its inception the Partnership includes new collaborations with RedX pharma and with Promega, as well as a substantial deepening of the collaboration with Carl Zeiss, and, with the support of the BG, we will organise annual outreach activities combined with our imaging day, aimed at broadening the industry user base.
3. The partnership will contribute to tackle important biomedical challenges, which will impact on society. Improvement of drug safety and use of nanomedicine, as well as increase understanding in pancreas disease, malaria, cancer progression and ageing will ultimately improve drug development for major disease treatment and hence well-being and health in the population. To maximise the impact on society and public awareness, the investigators will be involved in diverse communication activities, which were recently recognised by a BBSRC Excellence with Impact prize to the Institute of Integrative Biology in 2011.
Way to Impact: We have experience of staff visiting local schools, providing exposure to modern biology and encouragement for science careers (participation to Science fair by RL). Members of staff also attend University Open days, where they encourage young students to science. The CCI platform is one of the facilities shown to pupils and their families and provides very accessible visual tools for explaining scientific development. A further stage is through work with local schools and colleges (e.g. St Edwards College; Winstanley College; Range High School; Shorefields Technology College, Priestley College Warrington, the Princes Trust/ Mersey Fire and Rescue) and to give summer studentship placements supported by Nuffield Bursaries (RL, VS, AH, DB). Wider outreach ranges from a special "Murder Mystery" event at the Liverpool World Museum UoL (AH). RL has given several Christmas lectures for six-form students. These activities will be continued and expanded.

Publications

10 25 50
 
Title collaboration with music composer Emily and launch of PRISM 
Description PRiSM is the RNCM Centre for Practice & Research in Science & Music PRiSM brings together a number of creative collaborations between the sciences and music. PRisM was launched the 04/10/2017 with "The Music of Proof" performance 
Type Of Art Performance (Music, Dance, Drama, etc) 
Year Produced 2017 
Impact Dr Howard music composition has been influenced by discussion with mathematicians and biologists 
URL https://www.rncm.ac.uk/research/research-centres-rncm/prism/
 
Description A Dragonfly multimodal fast imaging platform with SRRF-stream (Super-Resolution Radial Fluctuation) in the Liverpool Centre for Cell Imaging (CCI)
Amount £450,000 (GBP)
Funding ID BB/R01390X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2018 
End 04/2019
 
Description Alert 13
Amount £247,156 (GBP)
Funding ID BB/L014947/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2014 
End 12/2016
 
Description Alert 14
Amount £380,884 (GBP)
Funding ID BB/M012441/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2015 
End 01/2016
 
Description DTP studentship
Amount £90,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2015 
End 09/2019
 
Description Development of New Mathematical Sciences for Healthcare Technologies
Amount £6,200,000 (GBP)
Funding ID EP/N014499/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 12/2015 
End 11/2019
 
Description Development of the chick embryo as a replacement for rodent models of tumour metastasis
Amount £240,000 (GBP)
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Private
Country United Kingdom
Start 03/2018 
End 02/2021
 
Description Evaluation of novel combination drug protocols for neuroblastoma using advanced imaging in a chick embryo model
Amount £117,000 (GBP)
Organisation North West Cancer Research (NWCR) 
Sector Charity/Non Profit
Country United Kingdom
Start 02/2018 
End 01/2020
 
Description MRC Clinical Research Capabilities and Technologies Initiative
Amount £5,000,000 (GBP)
Funding ID MR/M009114/1 
Organisation Medical Research Council (MRC) 
Department MRC Stratified Medicine
Sector Charity/Non Profit
Country United Kingdom
Start 04/2015 
End 03/2016
 
Description Training Grant
Amount £94,126 (GBP)
Funding ID BB/L016036/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 12/2014 
End 11/2018
 
Description Wellcome Trust - Multi-User Equipment Grant
Amount £600,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2014 
End 10/2019
 
Title 3D Printing Capabilities 
Description 3D Printing for outreach, fabrication and prototyping 
Type Of Material Improvements to research infrastructure 
Year Produced 2016 
Provided To Others? Yes  
Impact We have been using the 3D printer to prototype parts and objects to facilitate imaging technologies. This includes microscope parts such as filter holders, focus control interfaces, as well as more utility prints such as microscope slide boxes and holders to safely store microneedles for microinjection. This technology is also useful for demonstrating various aspects of research through 3D printing models of cells, spheroids, or basic microscopes (IE lens holders for mobile phone cameras). 
 
Title BioImage Analyst 
Description A BioImage Analyst was hired to support users of the Centre for Cell Imaging. By having a dedicated member of staff to aid in experimental design, acquisition and post-acquisition analysis techniques, data are acquired with analysis in mind. Furthermore a greater proportion of imaging data can produce reproducibly quantitative results. 
Type Of Material Improvements to research infrastructure 
Year Produced 2014 
Provided To Others? Yes  
Impact During the course of the grant, over 100 researchers (including on-site, external and commercial partners) have benefited from direct or indirect support and training. This has been delivered through one-on-one training with software and methods, training of small groups and hosting workshops on Image Analysis. Several projects have benefited from significant involvement and thus have led to authorship in published work (eg. https://doi.org/10.1098/rsob.160306 | https://doi.org/10.1098/rsob.160195 | https://doi.org/10.1038/srep24708 | https://doi.org/10.14293/s2199-1006.1.sor-chem.az1mju.v2 | http://dx.doi.org/10.1098/rsob.150277 | https://doi.org/10.1038/srep13601 ). The work done at the CCI has been made more visible through involvement in Image Analysis conferences, training schools and public outreach projects. 
URL http://cci.liv.ac.uk/
 
Title Epifluor 
Description Three main upgrades have been performed since installation of the Epifluorescent system in 2013: 1) Motorised stage: The addition of a motorised stage has vastly improved the functionality of the system, allowing for multi-point (XY) acquisition, as well as tile-scanning. This greatly enhances the efficiency of live cell imaging as many fields can be acquired during the same time course. 2) Colour Camera: The addition of an RGB camera to the spare sideport of the microscope has allowed users interested in crystalisation / birefringence of samples to capture more information than is possible on the greyscale EM-CCD camera. This also allows for the accurate recording of stained histological samples. 3) Computer Interface: The Microscope and ancillary systems have been interfaced with the acquisition PC. This allows multidimensional acquisitions to be programmed, including multiple channel, position and Z positions, as well as adding autofocus functionality. 
Type Of Material Improvements to research infrastructure 
Year Produced 2015 
Provided To Others? Yes  
Impact The epifluorescent microscope is a highly used piece of equipment. The addition of extra functionality has improved efficiency and increased the amount of data than can be acquired from an individual experiment (IE slide scanning 200+ fields instead of manually acquiring 10-20). The addition of a colour camera has also made available external collaborations with industrial partners including RedX Pharma and Unilever. 
URL http://cci.liv.ac.uk/equipment_epi.html
 
Title LSM780 
Description A new state-of-the-art confocal microscope has been installed in the Centre for Cell Imaging. It replaces a >10 year old instrument with a new one equiped with detectors offering much improved sensitivity, thereby allowing the use of lower laser power and shorter exposure time, substantially reducing the problems of photobleaching, phototoxicity and sample heating. This upgrade is critical for the CCI to continue to lead in long-term imaging of dynamic cellular processes and for detection of fluorescent fusion in cells at close to endogenous levels of expression, bypassing the over-expression hitherto required for their detection. The microscope can operate in photon counting mode, which enables correlative spectroscopy measurements such FCS, FCCS with no added module. The miscroscope is also equipped with a FLIM module for sensing of the intracellular environment such as Ca2+, O2, Reactive oxygen species (ROS) and metabolic state. Link below to Zeiss (microscope) URL. The FLIM module, connected to the instrument, has been procured from PicoQuant: http://www.picoquant.com/products/category/fluorescence-microscopes/lsm-upgrade-kit-compact-flim-and-fcs-upgrade-kit-for-lsms 
Type Of Material Improvements to research infrastructure 
Year Produced 2013 
Provided To Others? Yes  
Impact The instrument was installed in October 2013. During this first year of operation, the instrument has been mostly fully booked. The main users have been the groups of Violaine See, Daimark Bennett, Luning Liu in the Institute of Integrative Biology (Liverpool) and Chris Sanderson in the Institute of Translational Medicine (Liverpool). It is too early for publication outputs, but promising results have already been obtained by these four groups and further users are requesting access to the microscope. 
URL http://microscopy.zeiss.com/microscopy/en_gb/products/confocal-microscopes/lsm-780.html
 
Title Multi-User Equipment Grant entitled 'Supporting excellence in basic and clinical research: A flow cytometry/sorting and cell imaging platform for the genotypic and phenotypic analysis of Hazard Group 3 pathogens' 
Description Multi-User Equipment Grant entitled 'Supporting excellence in basic and clinical research: A flow cytometry/sorting and cell imaging platform for the genotypic and phenotypic analysis of Hazard Group 3 pathogens' We have set up to our knowledge the first dedicated HG3 imaging facility, able to sort and image HG3 pathogens, e.g. TB and HIV 
Type Of Material Technology assay or reagent 
Year Produced 2014 
Provided To Others? Yes  
Impact It has just been set up, impact will be described next year 
 
Title Photothermal microscope 
Description A microscope that enables detection of absorbing nanomaterials in live cells (e.g. gold) and tissue sections. 
Type Of Material Improvements to research infrastructure 
Year Produced 2011 
Provided To Others? Yes  
Impact Improvement to our understanding of non-fluorescent nanoparticles into cells (see publications 10.1371/journal.pone.0121683, 10.1098/rsos.140454) 
URL http://pcwww.liv.ac.uk/~cci/
 
Title Zegami 
Description A front-end visualisation platform for querying and interrogating imaging datasets. 
Type Of Material Improvements to research infrastructure 
Year Produced 2016 
Provided To Others? Yes  
Impact We have implemented a zegami server to act as a front-end to our public-facing OMERO gallery. This platform allows for the easy visualisation and interrogation of large imaging datasets and so provides an added layer of functionality. Several projects have utilised this system for Open-Data research projects, for an example see: https://doi.org/10.14293/s2199-1006.1.sor-chem.az1mju.v2 and corresponding data available at http://zegami.liv.ac.uk/2016-sf.html 
URL http://zegami.liv.ac.uk
 
Title epiflu 
Description A new epifluorescence microscope has been installed in the Centre for Cell Imaging in October 2013. This microscope (Zeiss) is equipped with incubators for long term cell imaging, as well as a fast and sensitive camera (Andor) and a micromanipulation and microinjection system (Narishige/Tritech/Eppendorf). 
Type Of Material Improvements to research infrastructure 
Year Produced 2013 
Provided To Others? Yes  
Impact This equipment has been in service for one year. In addition to my research group, it has been used by the groups of Violaine See, Daimark Bennett, and Luning Liu from the Institute of Integrative Biology (Liverpool) as well as by the group of Michael Griffiths (Institute of Global Health). It is still too early to list notable impacts from this instrument though a number of promising results have been obtained. One publication (Violaine See group) used the fast imaging camera was crucial to visualise cancer cells in the blood flow of a live chick embryo and establish the influence of hypoxia on their ability to invade the organism (intravasation) [10.1038/oncsis.2014.52]. The microscope is also used on a regular basis by the company RedX pharma. 
URL http://pcwww.liv.ac.uk/~cci/
 
Title OMERO 
Description A data repository and public data sharing platform based on the OMERO platform from the Open Microscopy Environment (OME) group at the University of Dundee. 
Type Of Material Database/Collection of data 
Year Produced 2014 
Provided To Others? Yes  
Impact The database is hosted on a 60TB file server providing archiving function to several groups using the Centre For Cell Imaging Shared Research Facility. Through collaboration with the developers we now offer a Public Facing repository where proprietary imaging data can be shared through a web browser without the need to install separate software. This has been utilised for Open Science Projects as well as a means to provide data supporting other published work. 
URL http://cci02.liv.ac.uk/gallery
 
Description Aurelia Bioscience 
Organisation Aurelia Bioscience
Country United Kingdom 
Sector Private 
PI Contribution Aurelia Bioscience is a research contract organisation. Their main source of revenues is large scale testing of compounds for toxicity or efficacy for the pharmaceutical companies. They are interested in 3D models as these might be more representative of in vivo systems. With the light sheet microscope, we have ran test experiments to demonstrate the possibility of visualising the fate of cells growing on the 3D scaffolds Aurelia Bioscience is developing. The very encouraging results have led to a successful BBSRC CASE application.
Collaborator Contribution Aurelia Bioscience procured the 3D scaffolds and grew various cell types. They came to Liverpool Centre for Cell Imaging to do the experiments with us. They are committing some additional funding through the CASE application.
Impact BBSRC Case Studentship to start October 2016 (Newcastle-Liverpool-Durham BBSRC Doctoral Training Partnership) The collaboration is highly multidisciplinary, involving physics, mathematics, data processing, biology, material chemistry and pharmacology.
Start Year 2015
 
Description CASE studentship on SuperResolution with Zeiss 
Organisation Carl Zeiss AG
Country Germany 
Sector Private 
PI Contribution We are developing/implementing various approaches to super resolution, currently mostly focused on SOFI.
Collaborator Contribution Zeiss microscopy provides technical support and scientific advice, as well as a placement (to take place later in the PhD) for the student.
Impact Interdisciplinary: physics, image analysis and processing, biology
Start Year 2014
 
Description Euro-Bioimaging 
Organisation Euro-BioImaging
Country European Union (EU) 
Sector Public 
PI Contribution Liverpool Centre for Cell Imaging has been recommended by a prestigious and independent international evaluation board as an advanced light microscopy Euro-Bioimaging node.
Collaborator Contribution Euro-BioImaging is a pan-European infrastructure project whose mission is to build a distributed imaging infrastructure across Europe that will provide open access to innovative biological and medical imaging technologies for European researchers. We are in the early phase of the process where "nodes" that can deliver this expertise are being identified.
Impact No output yet: it is expected that new users from UK, Europe and beyond will use our facilities in the coming months and years.
Start Year 2013
 
Description redX Onco Screening of in-house drugs libraries for amorphous -crystalline transitions via polarisation microscopy 
Organisation Redx Oncology Ltd
Country United Kingdom 
Sector Private 
PI Contribution We optimized a polarization microscopy assay to determine the solid state of pharmaceuticals from RedX pipeline.
Collaborator Contribution Providing the drug libraries and paying for using our facility.
Impact Outputs are fundamental in the drug development pipeline but cannot be specified in detail here due to the confidentiality policy of RedX. The microscopy assay complements thermodynamic, solubility and Liquid Chromatography tests performed in RedX laboratories.
Start Year 2014
 
Description workshop focused on cell imaging and image analysis 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact The Centre for Cell Imaging organised a 2 day workshop focused on light microscopy techniques and image analysis. 90 researchers and students attended the event mainly from Liverpool and Manchester but also from other Universities in the UK. Industrial partners were invited and the director of the Advanced Imaging Centre at the HHMI Janelia Research Campus, Teng-Leong Chew gave a keynote lecture and a comprehensive hands-on training session on Image Analysis.
Year(s) Of Engagement Activity 2017
URL https://cci.liv.ac.uk/2017_workshop.html
 
Description "Meet The Scientist" exhbit at the World Museum 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Our exhibit was entitled: "Seeing inside cells". More than 300 visitors stopped at our exhibit and learned about cells, and how we can use microscopy to understand how they function. Children have visualised cells using a fluorescent microscope.
Year(s) Of Engagement Activity 2015
URL https://www.liverpool.ac.uk/health-and-life-sciences/meet-the-scientists/21-november/
 
Description Hosting Lower 6th form summer students via the Nuffield scheme 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The Centre for Cell imaging has hosted 2 6th form students for 4 weeks as part of the Nuffield Foundation scheme
Year(s) Of Engagement Activity 2017
 
Description Hosting Lower 6th form summer students via the Nuffield scheme 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact A Lower 6th form summer student was hosted in the Institute and Centre for Cell Imaging via the Nuffield scheme. Results and experiences were presented and discussed at a sponsor meeting for staff, students and parents.
Year(s) Of Engagement Activity 2018
URL http://www.nuffieldfoundation.org/nuffield-research-placements
 
Description Image Analysis Blog 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact A blog, outreach and teaching resource based around the work done by Dave Mason, the CCI BioImage Analyst. The tutorials and educational material largely focuses on Open Source and Open Access tools such that others can apply the methods and techniques to their own quantification.

This platform also provides a route to engage with the wider community through comments and interactions on-site and via other social media platforms (IE twitter).
Year(s) Of Engagement Activity 2015,2016,2017
URL http://postaquisition.wordpress.com
 
Description Image Analysis Conference Live Tweeting 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Dave Mason (image analyst employed on the grant and now confirmed staff member of the Centre for Cell Imaging) live tweeted an image analysis conference and then wrote a blog post about live tweeting conferences. His tweets reached a broad community of potential users of the facility as well as other experts in the field. The blog post can encourage others to share their science directly with peers and with the public.
Year(s) Of Engagement Activity 2017
URL https://blogandlog.wordpress.com/2017/02/21/thoughts-on-livetweeting/
 
Description Imaging workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact More than 50 attendees participate to the yearly workshop organised by the Centre for Cell imaging. it provides training and awareness of new imaging technologies to group leaders, members of staff and students from the University of Liverpool, other Universities and local companies.
Year(s) Of Engagement Activity 2012,2013,2014,2015
URL http://pcwww.liv.ac.uk/~cci/2015imagingday.html
 
Description Imaging workshop September 2016 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Annual imaging workshop, focused on fundamentals in imaging and specific seminars on microscopy techniques such as super-resolution microscopy, intravital imaging, Life-time imaging. The main purpose is training and increasing awareness of the imaging capabilities in Liverpool
Year(s) Of Engagement Activity 2016
URL http://cci.liv.ac.uk/2016imagingday.html
 
Description Nuffield Summer Students 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Several of the co-Investigators hosted Nuffield Summer students in 2016. These provide a real experience of research and University to young people from various background who are selected for their achievements and enthusiasm for science. See hyperlink below for more details about the students and projects.
Year(s) Of Engagement Activity 2016
URL https://blogandlog.wordpress.com/2016/11/24/11-nuffield-students-visited-iib-this-summer/
 
Description Primary school visit for the Science week 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The activity consisted in 2x2h sessions with 2 year 6 classes in a primary school in Liverpool (Mosspits Lane primary school) for the Science week 2017. The children were offered activities focusing on scales in biology, use of microscopy and building their own magnifier.
Year(s) Of Engagement Activity 2017
 
Description School visit 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I gave an introductory lecture on molecular and cellular biology of cancer and have demonstrated how 3D/4D imaging has helped our understanding of cell migration. The students were fascinated and provided excellent feedback.
Year(s) Of Engagement Activity 2015
 
Description School visit to the CCI 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact 50 A-level students visited the Institute of Integrative Biology, and were given a talk about imaging in the 5 dimensions.
Year(s) Of Engagement Activity 2018
 
Description School visits 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact We have regular visits from groups from Secondary School in the Institute (in addition to the Open Days) and the visit of the Centre for Cell Imaging is always a high point as illustrated by the testimony from one of our visitor (see link below).
Year(s) Of Engagement Activity 2015,2016,2017
URL https://blogandlog.wordpress.com/2016/08/15/visit-from-a-level-biology-students/