Imaging microorganisms and pollutants at the microscale

Lead Research Organisation: Newcastle University
Department Name: Civil Engineering and Geosciences

Abstract

Fluorescence microscopy is a powerful tool for studying microorganisms in their natural environment without cultivation. The nucleic acids inside microbial cells can be stained with fluorescing dyes which specifically bind to targeted organisms. This technique can stain pollutant degrading microorganisms in natural samples for their identification, localisation and enumeration. Microbial degradation can break down and detoxify polycyclic aromatic hydrocarbons (PAHs). PAHs are carcinogenic pollutants present in organic liquids like crude oil or tar and also in numerous soils or sediments in the United Kingdom impacted by industrial activity. An interesting property of PAHs is their autofluorescence under ultraviolet light which means that they can be visualised with fluorescence microscopy. Fluorescence microscopy may thus be suitable for the simultaneous microscale imaging of PAH-rich patches in soils and sediments and their associated microbial communities. To this end criteria will be derived for the identification of PAH-rich patches in soil or sediment by fluorescence microscopy. Once the unambiguous distinction between the fluorescence of PAH-rich patches and other natural soil or sediment constituents has been achieved, a suitable methodology will be derived to stain and image the associated microorganisms using fluorescence in situ hybridisation (FISH), conventional epifluorescence microscopy and confocal laser scanning microscopy. The derived methodology could be used to study in detail and at the microscale the co-localisation of microorganisms and pollutants such as PAHs in the natural environment. A microscale investigation may shed light on the exact mechanisms controlling the microbial breakdown and attenuation of PAHs in polluted soils and sediments. Such insight is of fundamental importance for the design of bioremediation technologies and for the risk assessment for impacted ecosystems.

Publications

10 25 50
 
Description This project developed an analytical method to simultaneously image bacteria and nonaqueous pollutant phases (oil droplets etc) in soil.
Exploitation Route The new analytical techniques can be applied in the context of bioremediation to better understand association of pollutant liquids with pollutant degrading bacteria The new analytical technique can be applied to study oil biodegradation.
Sectors Environment

 
Description The images produced in this research are being used to illustrate the fascinating biogeochemical soil microenvironment in posters displayed during visit days. The research technician trained in this project has now become School Health and Safety Officer in the School of Engineering with responsibility for H&S of over 1000 staff and students.
First Year Of Impact 2017
Sector Environment
 
Description IAFRI - 3 year Phd for Vincenzo Padricello - Developing methodologies to understand the degradation and adsorption of macromolecule biopesticides in soil
Amount £57,880 (GBP)
Organisation Fera Science Limited 
Sector Multiple
Country United Kingdom
Start 09/2016 
End 08/2018
 
Description IMAGINE: INNOVATIVE TECHNOLOGIES FOR RAPIDLY SURVEYING, MAPPING AND COMMUNICATING WATERBORNE HAZARDS
Amount £477,153 (GBP)
Funding ID EP/P028527/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 05/2017 
End 04/2020
 
Description International joint project
Amount £11,600 (GBP)
Funding ID JP0869732 
Organisation The Royal Society 
Sector Academic/University
Country United Kingdom
Start 02/2009 
End 01/2011