The role of sortase-anchored proteins in the virulence of Streptococcus uberis

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Sortase (Srt) is a transamidase required to anchor proteins covalently at the surface of Gram-positive bacteria. Mutagenesis will be used to inactivate Srt activity in S. uberis. Proteomics will be used to identify proteins anchored at the bacterial surface through Srt. Interrogation of the genomic sequence will enable the orfs of Srt-anchored proteins to be identified and compared to a list of 30 putative sortase substrates, identified directly from the genome. The ability of srt mutant to display properties associated with virulence and to induce mastitis in the target species will be determined to identify the collective role of Srt-anchored proteins in the virulence of S. uberis and the pathogenesis of bovine mastitis.