The Biochemical and Cellular Characterisation of Histone Domethylases

Lead Research Organisation: University of Oxford
Department Name: Oxford Chemistry

Abstract

Epigenetics concerns the study of how gene expression is controlled, a process which modifies phenotypic traits while leaving the underlying genotype intact. Epigenetic mechanisms involve the dynamic regulation of DNA and histone modifications; while it is widely accepted that such modifications are linked to gene expression, gene silencing, and cell differentiation, the precise chemical mechanisms underpinning their regulation are undefined. Research on the enzymatic reactions regulating epigenetic modifications will further our understanding of epigenetics at the molecular level, and is essential for defining the physiology behind multifactorial diseases such as heart disease and cancer. Aims of the project are: 1) To compare demethylation kinetics of the KDMs with established histone substrates against novel substrates 2) To investigate the inhibition mechanisms and off-target effects of inhibitors developed to target only histone substrates 3) To explore the role of methylation on non-histone proteins. This project falls within the Physical sciences research theme.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
EP/N509711/1 01/10/2016 30/09/2021
1972092 Studentship EP/N509711/1 01/01/2016 31/12/2018 Joanna Bonnici