Investigating the effect of electric fields on cellular metabolism.

Lead Research Organisation: University of Warwick
Department Name: School of Life Sciences

Abstract

Metabolic overflow is the seemingly uneconomical process where metabolism shifts from the exclusive use of respiration to fermentation or respire-fermentation even in the presence of oxygen. Metabolic overflow is ubiquitous in nature, found in both microbial, mammalian and plant cells, and is shown to underpin higher-level processes such as cell growth, dormancy, and differentiation, and multi-cell communication and interactions. Despite this fundamental role, the molecular mechanisms leading to metabolic overflow and its relation to cellular biophysical parameters, such as membrane potential and cellular redox state remains unknown.
This project will study overflow metabolism using a combination of metabolic, electrophysiological, and fluorescent microscopy techniques, with the aim to better understand the relation between metabolic overflow, cellular redox state, and membrane potential. Investigations will use single-cell electrophysiological measurements such as pH, membrane potential and redox state in yeast and mammalian cells along with other cellular measurements such as ATP, NADH and metabolite measurements. This information will not only be vital to understand the molecular mechanisms of metabolic overflow, but will possibly pave the way to its manipulation through external electrical field stimulation.

Publications

10 25 50

Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M01116X/1 01/10/2015 30/09/2023
2097373 Studentship BB/M01116X/1 01/10/2018 30/09/2022 Emily Skates