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We show that HyPer, a yellow fluorescent protein can be successfully expressed in plant chloroplasts and used to report changes in hydrogen peroxide concentration. In this case a high light treatment increased hydrogen peroxide. The work shows that HyPer is functional in plants and can be used to elucidate signalling mechanisms associated with environmental stress responses.

First Author: Packer, Lester; Cadenas, Enrique

Attributed to: Development of plant-based hydrogen peroxide YFP nanosensors targeted to multiple sub-cellular locations funded by BBSRC

Abstract

No abstract provided

Bibliographic Information

Type: Book Chapter

Book Title: Hydrogen Peroxide and Cell Signaling: Part B (2013)

Page Reference: 185-201

ISBN: 978-0-12-405882-8