RFI Phase 2
Lead Research Organisation:
Rosalind Franklin Institute
Department Name: Research
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Organisations
Publications
Fritzsche M
(2022)
Author Correction: Live microscopy: cracking the challenge to image biology unfolding in cells, tissues, and organs.
in Communications biology
Ding Z
(2022)
Three-dimensional electron ptychography of organic-inorganic hybrid nanostructures.
in Nature communications
Levitz TS
(2022)
Approaches to Using the Chameleon: Robust, Automated, Fast-Plunge cryoEM Specimen Preparation.
in Frontiers in molecular biosciences
Buchanan CJ
(2022)
Pathogen-sugar interactions revealed by universal saturation transfer analysis.
in Science (New York, N.Y.)
Cobo I
(2022)
Probing Site-Selective Conjugation Chemistries for the Construction of Homogeneous Synthetic Glycodendriproteins.
in Chembiochem : a European journal of chemical biology
Hwang H
(2022)
Two-dimensional confinement for generating thin single crystals for applications in time-resolved electron diffraction and spectroscopy: an intramolecular proton transfer study.
in Chemical communications (Cambridge, England)
Perdigão L
(2022)
Computing for Optimized Biological Microscopy Data Processing and Analysis at The Rosalind Franklin Institute
in Microscopy and Microanalysis
Chatterjee G
(2022)
Torsionally broken symmetry assists infrared excitation of biomimetic charge-coupled nuclear motions in the electronic ground state.
in Chemical science
De Boer C
(2022)
Mechanism-based heparanase inhibitors reduce cancer metastasis in vivo.
in Proceedings of the National Academy of Sciences of the United States of America
Sun Y
(2022)
Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis.
in Science advances
Shen J
(2022)
Ultrafast photoinduced dynamics of a donor-([Formula: see text])bridge-acceptor based merocyanine dye.
in Scientific reports
Duan H
(2022)
Quantum coherent energy transport in the Fenna-Matthews-Olson complex at low temperature
in Proceedings of the National Academy of Sciences
Lin S
(2022)
Evidence that the Ser192Tyr/Arg402Gln in cis Tyrosinase gene haplotype is a disease-causing allele in oculocutaneous albinism type 1B (OCA1B)
in npj Genomic Medicine
Wu V
(2022)
High Resolution Ambient MS Imaging of Biological Samples by Desorption Electro-Flow Focussing Ionization.
in Analytical chemistry
Oltrabella F
(2022)
IPIP27A cooperates with OCRL to support endocytic traffic in the zebrafish pronephric tubule
in Human Molecular Genetics
Webby M
(2022)
Lipids mediate supramolecular outer membrane protein assembly in bacteria
in Science Advances
Chhabra M
(2022)
Structural Insights into Pixatimod (PG545) Inhibition of Heparanase, a Key Enzyme in Cancer and Viral Infections
in Chemistry - A European Journal
Strittmatter N
(2022)
Method To Visualize the Intratumor Distribution and Impact of Gemcitabine in Pancreatic Ductal Adenocarcinoma by Multimodal Imaging.
in Analytical chemistry
Parafioriti M
(2022)
Evidence for Multiple Binding Modes in the Initial Contact Between SARS-CoV-2 Spike S1 Protein and Cell Surface Glycans**
in Chemistry - A European Journal
Moynié L
(2022)
Hijacking of the Enterobactin Pathway by a Synthetic Catechol Vector Designed for Oxazolidinone Antibiotic Delivery in Pseudomonas aeruginosa
in ACS Infectious Diseases
Sakhapov D
(2022)
Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching.
in The journal of physical chemistry letters
Mollner T
(2022)
Reductive site-selective atypical C , Z -type/N2-C2 cleavage allows C-terminal protein amidation
in Science Advances
Morimoto K
(2022)
Cysteine Reactivity Profiling to Unveil Redox Regulation in Phytopathogens.
in Methods in molecular biology (Clifton, N.J.)
Enderlein J
(2022)
Modeling charge separation in charged nanochannels for single-molecule electrometry
in The Journal of Chemical Physics
King O
(2022)
Volume Segmantics: A Python Package for Semantic Segmentation of Volumetric Data Using Pre-trained PyTorch Deep Learning Models
in Journal of Open Source Software
Mikolajek H
(2022)
Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes.
in Proceedings of the National Academy of Sciences of the United States of America
Zaccai NR
(2022)
FCHO controls AP2's initiating role in endocytosis through a PtdIns(4,5)P2-dependent switch.
in Science advances
Fritzsche M
(2022)
Live microscopy: cracking the challenge to image biology unfolding in cells, tissues, and organs
in Communications Biology
Robinson A
(2022)
SIM-STEM Lab: Incorporating Compressed Sensing Theory for Fast STEM Simulation
in Ultramicroscopy
Duque-Correa M
(2022)
Defining the early stages of intestinal colonisation by whipworms
in Nature Communications
Bell C
(2022)
Trainable segmentation for transmission electron microscope images of inorganic nanoparticles
in Journal of Microscopy
Kennedy A
(2022)
Differences in CD80 and CD86 transendocytosis reveal CD86 as a key target for CTLA-4 immune regulation
in Nature Immunology
Royer C
(2022)
ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis.
in Nature communications
Nagoor O
(2022)
Sampling strategies for learning-based 3D medical image compression
in Machine Learning with Applications
Morimoto K
(2022)
Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme.
in The New phytologist
Armstrong Z
(2022)
Cryo-EM structures of human fucosidase FucA1 reveal insight into substrate recognition and catalysis
in Structure
Murray C
(2022)
Diamond: The Game - a board game for secondary school students promoting scientific careers and experiences
in Research for All
Halfon Y
(2022)
Maintaining the momentum in cryoEM for biological discovery.
in Faraday discussions
Kazantsev D
(2022)
High performance Savu software for fast 3D model-based iterative reconstruction of large data at Diamond Light Source
in SoftwareX
Alvarez-Borges F
(2022)
Comparison of Methods to Segment Variable-Contrast XCT Images of Methane-Bearing Sand Using U-Nets Trained on Single Dataset Sub-Volumes
in Methane
Pennington A
(2022)
SuRVoS 2: Accelerating Annotation and Segmentation for Large Volumetric Bioimage Workflows Across Modalities and Scales.
in Frontiers in cell and developmental biology
LuTheryn G
(2023)
Cationic Microbubbles for Non-Selective Binding of Cavitation Nuclei to Bacterial Biofilms.
in Pharmaceutics
Tamborrini D
(2023)
Structure of the native myosin filament in the relaxed cardiac sarcomere
in Nature
Krukauskas D
(2023)
Results from the Quantum C100, a Novel CMOS Detector Optimised for 100 keV Cryo Electron Microscopy
in Microscopy and Microanalysis
Rigby M
(2023)
Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens
in Cell Reports
O'Leary C
(2023)
Confessions of a Ptychopath: Detection, Dimensions, Damage and Despair
in Microscopy and Microanalysis
| Description | The Rosalind Franklin Institute phase 2 award covers the full scientific and research remit of this national institute. The award has taken the Institure through it's set up phase and into full operation, and has leveraged significant further funding for research, created new industry collaborations, and has seen the beginnings of an innovation and translation programme from our work. Our role is to create new techologies for life science. Within this phase 2 grant, we have pioneered the technique of cryo-epectron tomography, enabling the structures of biology to be seen within their biological context inside the cell, advanced physical sciences techniques into biology for the first time, and developed a world class laboratory for engineering biology which will bring precise control capabilities to the molecules of biology. The Institute has also been able to develop a sttdentship scheme, and now houses a cohort of ten stdents per year, in collabroation with Universities across the UK. |
| Exploitation Route | Our work can be translated into commercial products for imaging, or as theraputics. Our technolgies can be applied by academics or by industry to understand more about biological problems, and by working with instrument developers, new instruments are now on the market. |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| URL | https://www.rfi.ac.uk/about/annual-reports/ |
| Description | The Franklin has a significant community and public engagement programme, linked both to the work of the institute broadly, and to specific research areas. Our work in public engagement includes a training programme availabe to all staff, created in collaboration with Science Made Simple, opportunities throughout the year to engage local schools, in partnership with the Education Business Partnership, and Citzen science programmes intrinsic to our research activity created with Zooniverse. Working closely woth industry has realised significant non academic impact, including in the bring to market of new instrumentation, leverage by the phase 2 and Wellcome funded Electirfying Life Sciences Grant (e.g. the commercial FIB SEM 'Arctis'), through core activity with Bruker, and with JEOL. Full details on our engagement and non academic impact is available in our annual reports from www.rfi.ac.uk. |
| First Year Of Impact | 2022 |
| Sector | Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal Economic |
| Description | Challenge reviewer and contributor to Nurse RDI |
| Geographic Reach | National |
| Policy Influence Type | Contribution to a national consultation/review |
| Impact | The RDI review will shape government policy. |
| URL | https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/1140... |
| Description | Structural Biology: From Molecules to Cells. |
| Amount | £159,340 (GBP) |
| Funding ID | 099683 |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2012 |
| End | 09/2016 |
| Title | SINGLE DOMAIN ANTIBODIES THAT TARGET SARS-COV-2 |
| Description | The present invention relates to improved single domain antibodies that target SARS-CoV-2, multivalent polypeptides and fusion proteins comprising the single domain antibodies. The present invention also provides coronavirus binding molecules that bind two different epitopes on the receptor binding domain of a spike protein of a coronavirus. The coronavirus binding molecules are based on joining two antigen binding molecules together via a linker. The present invention provides the use of said single domain antibodies, multivalent polypeptides, fusion proteins and coronavirus binding molecules in treating and/or preventing coronavirus, as well as the use of said single domain antibodies, multivalent polypeptides, fusion proteins and coronavirus binding molecules in the detection and diagnosis of coronavirus using various methods, assays and kits. |
| IP Reference | WO2022053839 |
| Protection | Patent / Patent application |
| Year Protection Granted | 2022 |
| Licensed | Commercial In Confidence |
| Impact | The patent estate is in the final stages of agreement to licence. |
| Title | rosalindfranklininstitute/guanaco: |
| Description | No description provided. |
| Type Of Technology | Software |
| Year Produced | 2021 |
| Open Source License? | Yes |
| Impact | Shaping tomography practice |
| URL | https://zenodo.org/record/5185365 |
| Title | rosalindfranklininstitute/parakeet: |
| Description | Phantom generator |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | Imported into major software packages |
| URL | https://zenodo.org/record/6493567 |
| Description | Media work for science media centre |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I have supplied expert commentary on the pandemic and on science policy. |
| Year(s) Of Engagement Activity | 2020,2021,2022,2023 |
| URL | https://www.sciencemediacentre.org/?s=naismith&cat= |