RFI Phase 2
Lead Research Organisation:
Rosalind Franklin Institute
Department Name: Research
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Organisations
Publications
Zhang X
(2023)
Disentangling the complexity of coupled vibrations by two-dimensional electronic-vibrational spectroscopy
in Journal of Physics B: Atomic, Molecular and Optical Physics
Zaccai NR
(2022)
FCHO controls AP2's initiating role in endocytosis through a PtdIns(4,5)P2-dependent switch.
in Science advances
Yang N
(2024)
Cyclodextrin-based metal-organic frameworks transforming drug delivery
in European Journal of Medicinal Chemistry
Wu V
(2022)
High Resolution Ambient MS Imaging of Biological Samples by Desorption Electro-Flow Focussing Ionization.
in Analytical chemistry
Wells J
(2023)
N-Dimensional Dictionary Learning for Hyperspectral Scanning (Transmission) Electron Microscopy
in Microscopy and Microanalysis
Webby M
(2022)
Lipids mediate supramolecular outer membrane protein assembly in bacteria
in Science Advances
Warren AJ
(2024)
VMXm - A sub-micron focus macromolecular crystallography beamline at Diamond Light Source.
in Journal of synchrotron radiation
Vats K
(2024)
Ferroptosis of select skin epithelial cells initiates and maintains chronic systemic immune-mediated psoriatic disease.
in The Journal of clinical investigation
Vande Voorde J
(2023)
Metabolic profiling stratifies colorectal cancer and reveals adenosylhomocysteinase as a therapeutic target.
in Nature metabolism
Tiwari V
(2024)
Multitype Electronic Interactions in Precursor Solutions of Molecular Doped P3HT Polymer.
in The journal of physical chemistry. B
Tamborrini D
(2023)
Structure of the native myosin filament in the relaxed cardiac sarcomere
in Nature
Sun Y
(2022)
Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis.
in Science advances
Strittmatter N
(2022)
Method To Visualize the Intratumor Distribution and Impact of Gemcitabine in Pancreatic Ductal Adenocarcinoma by Multimodal Imaging.
in Analytical chemistry
Stoops T
(2025)
Obtaining 3D Atomic Reconstructions from Electron Microscopy Images Using a Bayesian Genetic Algorithm: Possibilities, Insights, and Limitations
in Microscopy and Microanalysis
Soenarjo A
(2023)
The Transition from Unfolded to Folded G-Quadruplex DNA Analyzed and Interpreted by Two-Dimensional Infrared Spectroscopy
in Journal of the American Chemical Society
Shen J
(2022)
Ultrafast photoinduced dynamics of a donor-([Formula: see text])bridge-acceptor based merocyanine dye.
in Scientific reports
Shad M
(2024)
Structural and functional insights of starch processing a-amylase from hyperthermophilic archaeon Pyrococcusabyssi.
in Carbohydrate research
Shad M
(2024)
Investigating the effect of SUMO fusion on solubility and stability of amylase-catalytic domain from Pyrococcus abyssi
in International Journal of Biological Macromolecules
Sekera ER
(2024)
Utilization of bis-MPA Dendrimers for the Calibration of Ion Mobility Collision Cross Section Calculations.
in Journal of the American Society for Mass Spectrometry
Schneider F
(2024)
Quantifying biomolecular organisation in membranes with brightness-transit statistics
in Nature Communications
Sakhapov D
(2022)
Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching.
in The journal of physical chemistry letters
Rödström K
(2024)
Extracellular modulation of TREK-2 activity with nanobodies provides insight into the mechanisms of K2P channel regulation
in Nature Communications
Royer C
(2022)
ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis.
in Nature communications
Robinson AW
(2024)
High-speed 4-dimensional scanning transmission electron microscopy using compressive sensing techniques.
in Journal of microscopy
Robinson A
(2022)
SIM-STEM Lab: Incorporating Compressed Sensing Theory for Fast STEM Simulation
in Ultramicroscopy
Robinson A
(2023)
Fast STEM Simulation Technique to Improve Quality of Inpainted Experimental Images Through Dictionary Transfer
in Microscopy and Microanalysis
Rigby M
(2023)
Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens
in Cell Reports
Richards LS
(2023)
Fragment-Based Ab Initio Phasing of Peptidic Nanocrystals by MicroED.
in ACS bio & med chem Au
Pražák V
(2024)
Molecular plasticity of herpesvirus nuclear egress analysed in situ
in Nature Microbiology
Pombo-García K
(2024)
Membrane prewetting by condensates promotes tight-junction belt formation
in Nature
Perdigão LMA
(2024)
RedLionfish - fast Richardson-Lucy Deconvolution package for efficient point spread function suppression in volumetric data.
in Wellcome open research
Perdigão L
(2022)
Computing for Optimized Biological Microscopy Data Processing and Analysis at The Rosalind Franklin Institute
in Microscopy and Microanalysis
Perbet R
(2024)
In situ seeding assay: A novel technique for direct tissue localization of bioactive tau.
in Journal of neuropathology and experimental neurology
Pennington A
(2022)
SuRVoS 2: Accelerating Annotation and Segmentation for Large Volumetric Bioimage Workflows Across Modalities and Scales.
in Frontiers in cell and developmental biology
Pei X
(2023)
Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer
in Nature Communications
Pasqualetto G
(2023)
CryoEM structure and Alphafold molecular modelling of a novel molluscan hemocyanin.
in PloS one
Parafioriti M
(2022)
Evidence for Multiple Binding Modes in the Initial Contact Between SARS-CoV-2 Spike S1 Protein and Cell Surface Glycans**
in Chemistry - A European Journal
Oltrabella F
(2022)
IPIP27A cooperates with OCRL to support endocytic traffic in the zebrafish pronephric tubule
in Human Molecular Genetics
O'Leary C
(2023)
Confessions of a Ptychopath: Detection, Dimensions, Damage and Despair
in Microscopy and Microanalysis
Nicholls D
(2024)
The Potential of Subsampling and Inpainting for Fast Low-Dose Cryo FIB-SEM Imaging.
in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Nicholls D
(2023)
Compressive Cryo FIB-SEM Tomography
in Microscopy and Microanalysis
Naseer Khan RM
(2023)
Distributable, Metabolic PET Reporting of Tuberculosis.
in bioRxiv : the preprint server for biology
Naismith J
(2023)
Getting the Most Out of Your Sample from SEM for TEM
in Microscopy and Microanalysis
Nagoor O
(2022)
Sampling strategies for learning-based 3D medical image compression
in Machine Learning with Applications
Mycroft-West C
(2025)
Structural glycobiology - from enzymes to organelles
in Biochemical Society Transactions
| Description | The Rosalind Franklin Institute phase 2 award covers the full scientific and research remit of this national institute. The award has taken the Institure through it's set up phase and into full operation, and has leveraged significant further funding for research, created new industry collaborations, and has seen the beginnings of an innovation and translation programme from our work. Our role is to create new techologies for life science. Within this phase 2 grant, we have pioneered the technique of cryo-epectron tomography, enabling the structures of biology to be seen within their biological context inside the cell, advanced physical sciences techniques into biology for the first time, and developed a world class laboratory for engineering biology which will bring precise control capabilities to the molecules of biology. The Institute has also been able to develop a sttdentship scheme, and now houses a cohort of ten stdents per year, in collabroation with Universities across the UK. |
| Exploitation Route | Our work can be translated into commercial products for imaging, or as theraputics. Our technolgies can be applied by academics or by industry to understand more about biological problems, and by working with instrument developers, new instruments are now on the market. |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| URL | https://www.rfi.ac.uk/about/annual-reports/ |
| Description | The Franklin has a significant community and public engagement programme, linked both to the work of the institute broadly, and to specific research areas. Our work in public engagement includes a training programme availabe to all staff, created in collaboration with Science Made Simple, opportunities throughout the year to engage local schools, in partnership with the Education Business Partnership, and Citzen science programmes intrinsic to our research activity created with Zooniverse. Working closely woth industry has realised significant non academic impact, including in the bring to market of new instrumentation, leverage by the phase 2 and Wellcome funded Electirfying Life Sciences Grant (e.g. the commercial FIB SEM 'Arctis'), through core activity with Bruker, and with JEOL. Full details on our engagement and non academic impact is available in our annual reports from www.rfi.ac.uk. |
| First Year Of Impact | 2022 |
| Sector | Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal Economic |
| Description | Challenge reviewer and contributor to Nurse RDI |
| Geographic Reach | National |
| Policy Influence Type | Contribution to a national consultation/review |
| Impact | The RDI review will shape government policy. |
| URL | https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/1140... |
| Description | Structural Biology: From Molecules to Cells. |
| Amount | £159,340 (GBP) |
| Funding ID | 099683 |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2012 |
| End | 09/2016 |
| Title | SINGLE DOMAIN ANTIBODIES THAT TARGET SARS-COV-2 |
| Description | The present invention relates to improved single domain antibodies that target SARS-CoV-2, multivalent polypeptides and fusion proteins comprising the single domain antibodies. The present invention also provides coronavirus binding molecules that bind two different epitopes on the receptor binding domain of a spike protein of a coronavirus. The coronavirus binding molecules are based on joining two antigen binding molecules together via a linker. The present invention provides the use of said single domain antibodies, multivalent polypeptides, fusion proteins and coronavirus binding molecules in treating and/or preventing coronavirus, as well as the use of said single domain antibodies, multivalent polypeptides, fusion proteins and coronavirus binding molecules in the detection and diagnosis of coronavirus using various methods, assays and kits. |
| IP Reference | WO2022053839 |
| Protection | Patent / Patent application |
| Year Protection Granted | 2022 |
| Licensed | Commercial In Confidence |
| Impact | The patent estate is in the final stages of agreement to licence. |
| Title | rosalindfranklininstitute/guanaco: |
| Description | No description provided. |
| Type Of Technology | Software |
| Year Produced | 2021 |
| Open Source License? | Yes |
| Impact | Shaping tomography practice |
| URL | https://zenodo.org/record/5185365 |
| Title | rosalindfranklininstitute/parakeet: |
| Description | Phantom generator |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | Imported into major software packages |
| URL | https://zenodo.org/record/6493567 |
| Description | Media work for science media centre |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I have supplied expert commentary on the pandemic and on science policy. |
| Year(s) Of Engagement Activity | 2020,2021,2022,2023 |
| URL | https://www.sciencemediacentre.org/?s=naismith&cat= |