Impact of Hedgehog signalling on the T cell receptor (TCR) repertoire
Lead Research Organisation:
University College London
Department Name: Cell and Developmental Biology
Abstract
We have shown that the Hedgehog signalling pathway regulates T-cell development in the thymus1,2. Sonic hedgehog (Shh) from thymic epithelial cells (TEC) signals to developing thymocytes to regulate their differentiation at key pre-T-cell receptor (pre-TCR) and TCR-dependent check-points1,2. Analysis of TCR-transgenic mice showed that Hh signalling reduced TCR signal strength during pre-TCR and TCR-induced differentiation2,3. We now plan to test the impact of these changes in TCR-signal strength induced by Hedgehog signalling on TCR repertoire selection, by next generation sequencing the TCR and TCR-chain repertoires from facs-sorted thymocyte populations at different stages of their development.
Organisations
People |
ORCID iD |
Tessa Crompton (Primary Supervisor) | |
Jasmine Rowell (Student) |
Publications
Yánez DC
(2021)
Systemic Pharmacological Smoothened Inhibition Reduces Lung T-Cell Infiltration and Ameliorates Th2 Inflammation in a Mouse Model of Allergic Airway Disease.
in Frontiers in immunology
Solanki A
(2020)
The transcriptional repressor Bcl6 promotes pre-TCR-induced thymocyte differentiation and attenuates Notch1 activation.
in Development (Cambridge, England)
Mengrelis K
(2019)
Sonic Hedgehog Is a Determinant of ?d T-Cell Differentiation in the Thymus.
in Frontiers in immunology
Lau CI
(2021)
The pioneer transcription factors Foxa1 and Foxa2 regulate alternative RNA splicing during thymocyte positive selection.
in Development (Cambridge, England)
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/M009513/1 | 01/10/2015 | 31/03/2024 | |||
1903458 | Studentship | BB/M009513/1 | 01/10/2017 | 02/03/2022 | Jasmine Rowell |