Using microscale technologies in tissue engineering of human lung

Lead Research Organisation: University of Nottingham
Department Name: Sch of Molecular Medical Sciences


United States


10 25 50
Description During this project we explored the use of 3D hydrogel scaffolds in developing co-cultures of human immune cells and structural cells such as lung epithelium. Key skills developed during this sabbatical include use of photo cross-linking in micropatterning, cell encapsulation and developing 3D hydrogel based scaffolds. Such techniques complemented other strategies we use in developing 3D tissue models including the immunocompetent lung model we are developing as part of a BBSRC funded project. With help from my host research group at Harvard University we have now established the technology for fabrication of microppatterned hydrogels in our laboratory at the University of Nottingham. Such micropatterned scaffolds underpin a number of research projects within my laboratory. Another skill developed during this sabbatical was fabrication of microwells that enable establishing microscale single and co-cultures. Activities during this sabbatical also led to securing major research grant from the European Commission.
Exploitation Route Developing 3D immune competent tissue models for disease modelling
Sectors Pharmaceuticals and Medical Biotechnology

Description This ISIS visit led to establishing a substantive collaboration between research groups at the University of Nottingham and Harvard Medical School. This has already led to a number of ongoing collaborative projects some of which are funded by EU or NIH. We expect this will continue to generate more outputs in areas of immune competent tissue models and immune-bioengineering.
First Year Of Impact 2011
Sector Pharmaceuticals and Medical Biotechnology
Impact Types Societal

Description Construction of a miniaturized human lymph node model as an alternative to the Local Lymph Node Assay
Amount £120,000 (GBP)
Funding ID NC/K500318/1 
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2012 
End 08/2016
Description Local Immunomodulation around implants by innovative auxiliary hydrogel-based systems encapsulating autologous and phenotype controlled macrophages.
Amount £5,600,000 (GBP)
Funding ID 602694 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 09/2013 
End 09/2017
Description Personalized And/Or Generalized Integrated Biomaterial Risk Assessment
Amount € 7,992,471 (EUR)
Funding ID 760921 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 12/2017 
End 01/2022
Description A microfluidic device for studying innate-adaptive immune cell interaction 
Organisation Brigham and Women's Hospital
Country United States 
Sector Hospitals 
PI Contribution Using a microfluidic device to establish a multi cell culture of immune cells under flow conditions
Start Year 2012
Description Investigating the immunological properties of hydrogels 
Organisation Brigham and Women's Hospital
Country United States 
Sector Hospitals 
PI Contribution A number of collaborative projects have been instigated to analyse the immuno-compatibility of different hydrogels in collaboration with collaborators at Harvard Medical School
Start Year 2012