More than skin deep: from blood to hair and return. In vivo imaging and functional manipulation of stem cell niches.
Lead Research Organisation:
Imperial College London
Department Name: Life Sciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Batista S
(2014)
Haematopoietic focal adhesion kinase deficiency alters haematopoietic homeostasis to drive tumour metastasis.
in Nature communications
Carlson AL
(2013)
Tracking single cells in live animals using a photoconvertible near-infrared cell membrane label.
in PloS one
Duarte D
(2018)
Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML.
in Cell stem cell
Engert A
(2016)
The European Hematology Association Roadmap for European Hematology Research: a consensus document.
in Haematologica
Hawkins ED
(2016)
T-cell acute leukaemia exhibits dynamic interactions with bone marrow microenvironments.
in Nature
Joseph C
(2013)
Deciphering hematopoietic stem cells in their niches: a critical appraisal of genetic models, lineage tracing, and imaging strategies.
in Cell stem cell
Khorshed R
(2014)
Conference contribution
Khorshed R
(2016)
Microscopy and Analysis
Khorshed RA
(2015)
Automated Identification and Localization of Hematopoietic Stem Cells in 3D Intravital Microscopy Data.
in Stem cell reports
Rashidi NM
(2014)
Flying back to the nest: Intravital microscopy reveals how the niche can induce stemness.
in Intravital
| Description | We have developed communal strategies to be applied to intravital microscopy of skin and bone marrow. Moreover, we have successfully implemented laser-mediated cell ablation in the bone marrow and we are now exploring alternative transgenesis methods and intravital cell signalling reporter strategies. |
| Exploitation Route | publications and further funding applications |
| Sectors | Healthcare |
| Description | FILM microscopy facility: New generation confocal and high content screening microscopes, and upgrades for intravital microscopy |
| Amount | £590,993 (GBP) |
| Funding ID | 104931/Z/14/Z |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2015 |
| End | 12/2020 |
| Description | In vivo analysis of the interactions between acute myeloid leukaemia, T cells and haematopoietic stem cells to inform the design of T cell immunotherapy protocols. |
| Amount | £253,000 (GBP) |
| Organisation | Bloodwise |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2016 |
| End | 01/2019 |
| Description | In vivo and in silico mapping of cell-cell interactions in the haematopoietic stem cell niche |
| Amount | £860,000 (GBP) |
| Funding ID | BB/L023776/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2014 |
| End | 09/2017 |
| Title | Longitudinal intravital microscopy of mouse bone marrow |
| Description | precise repositioning of animals in intravital microscopy set up to achieve tissue-wide monitoring of haematopoietic events. It reduces the number of animals needed to comnduct our research |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | Publication currently under revision |
| Description | Valentina Greco - laser mediated cell ablation |
| Organisation | Yale University |
| Department | School of Medicine |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We provided training on intravital microscopy set up |
| Collaborator Contribution | We received training in laser mediated photoablation, which we are now applying to the HSC niche |
| Impact | manuscripts in preparation |
| Start Year | 2014 |
| Description | BBC radio interview |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Dr. Cristina LoCelso was interviewed regarding her Nature publication regarding tracking leukaemia-resistant cells. |
| Year(s) Of Engagement Activity | 2016 |
| URL | https://www.thenakedscientists.com/articles/interviews/tracking-leukaemia-resistant-cells |
| Description | Blood culture |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Wellcome trust funded, multi-awarded radio drama revolving around sickle cell anaemia, blood and bone marrow transplant topics |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.blood-culture.com/ |
| Description | MedSciLives |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Interviewed for the MedSciLife posts of the Academy of Medical Sciences |
| Year(s) Of Engagement Activity | 2017 |
| Description | NatureJobs featured article |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | I was interviewed and the piece published by Nature Jobs, and noticed internationally |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://blogs.nature.com/naturejobs/2018/01/10/cristina-lo-celso-career-changing-encounters/ |
| Description | Press release |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Imperial College London press release |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/newssummary/news_17-10-2016-14-3-54 |
| Description | Women at Imperial |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Many people attended our stand and undertook the activities we proposed |
| Year(s) Of Engagement Activity | 2015 |