More than skin deep: from blood to hair and return. In vivo imaging and functional manipulation of stem cell niches.

Lead Research Organisation: Imperial College London
Department Name: Life Sciences

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
 
Description We have developed communal strategies to be applied to intravital microscopy of skin and bone marrow.
Moreover, we have successfully implemented laser-mediated cell ablation in the bone marrow and we are now exploring alternative transgenesis methods and intravital cell signalling reporter strategies.
Exploitation Route publications and further funding applications
Sectors Healthcare

 
Description FILM microscopy facility: New generation confocal and high content screening microscopes, and upgrades for intravital microscopy
Amount £590,993 (GBP)
Funding ID 104931/Z/14/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2015 
End 12/2020
 
Description In vivo analysis of the interactions between acute myeloid leukaemia, T cells and haematopoietic stem cells to inform the design of T cell immunotherapy protocols.
Amount £253,000 (GBP)
Organisation Bloodwise 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2016 
End 01/2019
 
Description In vivo and in silico mapping of cell-cell interactions in the haematopoietic stem cell niche
Amount £860,000 (GBP)
Funding ID BB/L023776/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2014 
End 09/2017
 
Title Longitudinal intravital microscopy of mouse bone marrow 
Description precise repositioning of animals in intravital microscopy set up to achieve tissue-wide monitoring of haematopoietic events. It reduces the number of animals needed to comnduct our research 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact Publication currently under revision 
 
Description BBC radio interview 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Dr. Cristina LoCelso was interviewed regarding her Nature publication regarding tracking leukaemia-resistant cells.
Year(s) Of Engagement Activity 2016
URL https://www.thenakedscientists.com/articles/interviews/tracking-leukaemia-resistant-cells
 
Description Blood culture 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Wellcome trust funded, multi-awarded radio drama revolving around sickle cell anaemia, blood and bone marrow transplant topics
Year(s) Of Engagement Activity 2017
URL http://www.blood-culture.com/
 
Description MedSciLives 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Interviewed for the MedSciLife posts of the Academy of Medical Sciences
Year(s) Of Engagement Activity 2017
 
Description NatureJobs featured article 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was interviewed and the piece published by Nature Jobs, and noticed internationally
Year(s) Of Engagement Activity 2017
URL http://blogs.nature.com/naturejobs/2018/01/10/cristina-lo-celso-career-changing-encounters/
 
Description Press release 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact Imperial College London press release
Year(s) Of Engagement Activity 2016
URL http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/newssummary/news_17-10-2016-14-3-54
 
Description Women at Imperial 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Policymakers/politicians
Results and Impact Many people attended our stand and undertook the activities we proposed
Year(s) Of Engagement Activity 2015