Visit to the laboratory of Professor Alison Barth to learn in vivo optogenetics, multicellular recording and re-establish the collaboration

Lead Research Organisation: Keele University
Department Name: Inst for Science and Tech in Medicine

Abstract

United States

Publications

10 25 50
 
Description Re-established collaboration. New experiments planned in the summer 2017, which have been moved to January 2018 and executed. Plans have been delineated for the summer visit (2018)
Set-up optogenetic in vivo at Keele and in vitro at Aston and executed all experiments that required technology learned at Carnegie Mellon, including controls. All of them have been listed in the parallel grant portfolio (in vivo and in vitro optogenetics).
Calcium recording in vivo learned in Barth lab is necessary to met the objectives of the next BBSRC grant that we have just applied for, but were turned down. Also executed a few experiments with use of silicon probes gaining preliminary data for the grant application.
Exploitation Route We are using technology that we learned at Carnegie Mellon in our present and we will use in our future approaches, that is optogenetics, calcium imaging in vivo and silicon probes recording. We see the interest in this technology from the surrounding labs and we are willing to help in the matter. WE also started collaboration with a group from the Nencki Institute , Warsaw, requiring optogenetics. This new collaboration enable us also to learn functional optical recoding.
Sectors Education,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description increasing our general understanding of brain function
First Year Of Impact 2015
Sector Education
Impact Types Societal

 
Description Travel Grant
Amount £698 (GBP)
Organisation Physiological Society 
Sector Charity/Non Profit
Country Global
Start 08/2018 
End 09/2018
 
Title new method of stimulating astrocytes via IP3R using opto - and chemogenetics 
Description optogenetic via Gq coupled melanopsin (OPN-GFAP-CNP) and a chemo-genetic via "Designer Receptor Exclusively Activated by Designer Drug" with GFAP promoter (GFAP-Gq-DREADD) can be used to target astrocyte Gq-IP3 signalling 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact using these methods one can drive astrocytes via their major known pathways 
 
Description Cortical representation of temperature in rodents 
Organisation University of Notre Dame
Department Department of Biological Sciences
Country United States 
Sector Academic/University 
PI Contribution Hands on expertise in extracellular recording in vivo, including the surgical skills and the ideas, part of subsistence and travel support.
Collaborator Contribution Ideas, animals, consumables, part of subsistence and travel support
Impact This is the collaboration in frame of neuroscience, but still probably multidisciplinary. This is on-going research. We have done several recordings for the mice insular and somatosensory cortices and also from the superior colliculus. Only in the latter location we managed to find neurones responding to cold temperature. WE are at present moving with this project to Arch optogenetics and also multicellular recording from insula.
Start Year 2012
 
Description Development of a classifier to differentiate between classes of neurones during in vivo recording 
Organisation Carnegie Mellon University
Department Department of Biological Sciences
Country United States 
Sector Academic/University 
PI Contribution The collaboration re-started at the beginning of this year with solving technical issues associated with silicon probe recordings in vivo. This collaboration can take many different routes.
Collaborator Contribution participation in the cost of the visit to Pittsburgh and offering brilliantly equipped lab and technical help.
Impact Solving technical issues associated with silicon probe recording
Start Year 2018
 
Description Effects of learning involving vibrissae on responses across the layers of the barrel cortex 
Organisation Carnegie Mellon University
Department Department of Biological Sciences
Country United States 
Sector Academic/University 
PI Contribution Taking part in setting up and executing the experiments
Collaborator Contribution Provided animals, consumables and training and covered the cost of transportation and subsistence plus the leading role during setting up and the execution of all parts of the experiments
Impact This is interdisciplinary collaboration. Some results are already collected and they are processed at present
Start Year 2018
 
Description Mechanisms of plasticity in the mouse barrel cortex 
Organisation Cardiff University
Country United Kingdom 
Sector Academic/University 
PI Contribution Electrophysiological recordings from wt and genetically modified mice in vivo, travel money, consumables, lab work
Collaborator Contribution genetically modified and wt mice, some recordings, travel and accommodation money
Impact Journal Papers Fox K. Wright N., Wallace H. and Glazewski S. (2003) The origin of cortical surround receptive fields studied in the barrel cortex. J. Neurosci. 23: 8380-8391. *Hardingham N.R., *Glazewski S., *Pakhotin P., Giese K.P., Chapman P. and Fox K. (2003) The effect of a CaMKII T286A point mutation on experience-dependent plasticity and LTP in mouse barrel cortex. J. Neurosci., 23: 4428-4436.*shared first authorship. Wright N., Glazewski S. Hardingham N., Philips K., Pervolaraki E. and Fox K. (2008) Laminar analysis of the role of GluR1 in experience-dependent and synaptic depression in barrel cortex. Nature Neurosci. 11: 1140-1142. Dachtler J., Hardingham N., Glazewski S., Wright N., Blain E., Fox K. (2011) Experience-dependent plasticity acts via GluR1 and a novel NOS1-dependent synaptic mechanism in adult cortex. J. Neurosci. 31: 11220-11230. Meeting papers: Glazewski S., Sprengel R. and Fox K. The effect of deleting AMPA receptor subunit GluR A on experience-dependent plasticity in mouse barrel cortex. Society for Neuroscience Annual Meeting, Orlando 2002. Fox K, Wright N., Wallace H and Glazewski S. The origin of cortical surround receptive fields studied in the barrel field. Barrel Meeting, Lausanne, 2004. Dachtler, J.; Wright, N. F.; Glazewski, S.; Fox, K. D. The role of neuronal nitric oxide in experience-dependent plasticity in the mouse barrel cortex. Society for Neuroscience Annual Meeting, Washington D.C, 2008. Dachtler J., Hardingham N. R., Glazewski S.; Fox K. D. Gender differences in neuronal nitric oxide synthase-dependent neocortical plasticity. Society for Neuroscience Annual Meeting, Chicago, 2009. Dachtler, J.; Wright, N. F.; Glazewski, S.; Fox, K. D. The role of neuronal nitric oxide in experience-dependent plasticity in the mouse barrel cortex. Society for Neuroscience Annual Meeting, Washington D.C, 2008. Glazewski, S., Greenhill, S., Fox, K. Experience-dependent Homeostatic plasticity in the barrel cortex. Society for Neuroscience Annual Meeting, San Diego, 2013. Glazewski, S., Greenhill, S., Butcher, J., Fox, K. Experience-dependent Homeostatic plasticity in the barrel cortex. Forum of European Neuroscience, Milan, Italy, 2014.
Start Year 2006
 
Description Brain Awareness Week in Krakow and Lublin, Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Brain Awareness Week in Krakow and Lublin. Individual lectures in frame of this event in Krakow with media attendance and broad spectrum of the public (well over 500 participants in each case). The purpose was to familiarise the public with recent achievements in chosen areas of neuroscience, including the newest technology, prospects, impact on society and medicine. This was followed by the discussion and media interview.
Year(s) Of Engagement Activity 2007,2009,2015
 
Description School visits (two schools..one comprehensive and one grammar) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Different forms of activities. Mostly lecturing followed by the discussion, questions and answers. Sometimes demonstrations. The aim is to popularise neuroscience.

not measurable
Year(s) Of Engagement Activity 2012,2013,2014,2015,2016
 
Description Speaker in frame of 13th Electrophysiological Conference, Warsaw , Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Formal talk to professionals including the data collected during both grants
Year(s) Of Engagement Activity 2018
 
Description Talk in Carnegie Mellon University 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact The talk about homeostatic plasticity with the discussion
Year(s) Of Engagement Activity 2018
 
Description Teaching Mobility Lublin 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact 8 hours of lecturing delivered in frame of Erasmus Teaching Mobility with discussion afterwards plus exchange on research and teaching
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014,2015,2016,2017,2018
 
Description Teaching Mobility Lublin 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact 8h of lecturing (neuroscience, including the role of astrocytes in various processes in the CNS)...50-70 people attending each of them
Year(s) Of Engagement Activity 2016
 
Description University visit in frame of Teaching Mobility (Krakow and Lublin, Poland) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Series of lectures concerning different aspects of neuroscience (each year the different set) and in many cases linked to the understanding every day human activities. The lectures also includes large technological components, including their possible impact ion every day life. There is always long discussion at the end of each lectures followed by private talks. The main purpose is to popularise neuroscience , which is a rarity in Poland

not measurable
Year(s) Of Engagement Activity 2008,2009,2010,2011,2012,2013,2014,2015,2016