Novel enzyme diversity for improving cleaning and hygiene
Lead Research Organisation:
UNIVERSITY OF EXETER
Department Name: Biosciences
Abstract
The human body produces up to gram quantities each day of sebum that is deposited on our clothes. The common
enzymes added to many washing powders are protease and lipase enzymes to digest the proteins and lipids on the wash
fabric. The sebum contains a complex mixture of free fatty acids, triglycerides and cholesterol esters, wax esters, and
squalene. much of which remains on the clothes after the wash. In addition there is a need to remove resistant stains such
as tea stains which are difficult to remove without some bleaching of the fabric.
The are other enzyme activities which exist in nature that can breakdown these other products and also enzymes which
have a bleaching effect.
The project will aim to identify and characterise these enzymes from a large DNA resource that has been isolated from hot
environments around the globe. This resource can be mined using bioinformatic techniques to identify enzyme classes that
will tackle these cleaning problems. This will allow the production of small amounts of the new robust enzymes to be
produced for testing at the partner company, Unilever. In addition libraries of small pieces of DNA from different
thermophilic genomes have be made as libraries that can be screening for activity of the enzymes again specific fatty acids
etc. The overall project will allow access to novel stable new enzymes that could be used to improve the overall cleaning
process.
The use of enzymes in these processes is environmentally friendly and avoids the use of harsh bleaching agents.
enzymes added to many washing powders are protease and lipase enzymes to digest the proteins and lipids on the wash
fabric. The sebum contains a complex mixture of free fatty acids, triglycerides and cholesterol esters, wax esters, and
squalene. much of which remains on the clothes after the wash. In addition there is a need to remove resistant stains such
as tea stains which are difficult to remove without some bleaching of the fabric.
The are other enzyme activities which exist in nature that can breakdown these other products and also enzymes which
have a bleaching effect.
The project will aim to identify and characterise these enzymes from a large DNA resource that has been isolated from hot
environments around the globe. This resource can be mined using bioinformatic techniques to identify enzyme classes that
will tackle these cleaning problems. This will allow the production of small amounts of the new robust enzymes to be
produced for testing at the partner company, Unilever. In addition libraries of small pieces of DNA from different
thermophilic genomes have be made as libraries that can be screening for activity of the enzymes again specific fatty acids
etc. The overall project will allow access to novel stable new enzymes that could be used to improve the overall cleaning
process.
The use of enzymes in these processes is environmentally friendly and avoids the use of harsh bleaching agents.
Technical Summary
The technical approach is to use both bioinformatics mining and the screening of existing genomic libraries from the large DNA resource from thermophilic organisms and metagenomes accumulated from the recently completed EU Hotzyme project and other 'in house' resources available. The human sebum which is secreted daily from our bodies in not completely removed with the existing detergent enzymes. With knowledge of the human sebum composition the project will focus on the identification of the following 4 enzyme activities: (1) Novel carboxyl esterases and lipases since the conversion of triglycerides (TGs) to fatty acids found in sebum is only 70 % complete since the remaining 30% of TGs are non-saturated fatty acids that have poor affinity for the active site of the currently characterised TG lipases that are currently used in detergents. This is due to conformational restrictions of the cis-double bonds hindering the TG removal since the fatty alcohols do not carry a charge, which reduces the enzymes affinity for carboxylic acid products, which are likely to stay in the enzyme active site. (2) Cholesterol esterases - the Exeter group has previously solved a 3D structure of a novel potentially related esterase (Bourne et al.,2000). New novel cholesterol esterases will be mined from the genomic and metagenomics database. (3) Perhydrolases converting the available free fatty acids or carboxylic ester into an organic per-acid in the presence of hydrogen peroxide. This alpha/betahydrolase family member will contribute to the removal of superior sebum remnants and an improved hygiene by generating per-acetic acid for stain bleaching and will support the oxidation of the unsaturated fatty acids and triglycerides. (4) Squalene-hopene cyclase - converting squalene that is widely present in the human sebum into the sterol-like compound hopene.
Planned Impact
The enzymes discovered as part of this project will have an impact on the application biocatalysis in the industrial setting.
The development of new enzymes for fabric cleaning will benefit the general public as a whole. It will improve health and
overall quality of life. It will contribute to a sustainable economy.
As the world population steadily increases - from 7 billion in 2011 to forecasted 9 billion in 2030 (www.un.org) with people
migrating to the cities, the demand for water and other resources will multiply. By 2020, it is assumed that 1.4 billion people
will be living in urban areas, that is, 40% more than today. This strongly increases the risk for human hygiene and health
and severely impacts on the ecological and environmental balance. In this context, it is expected that more cleaning
products will be needed, for either hand or automated cleaning systems, which require more energy and more water while
producing more waste using the currently established cleaning techniques/methods. Also, higher living standards will
increase the demand for related products, notably laundry/cleaning products, per household and year, yielding more wash
cycles done per household and year. As a result, more frequent usage of warm wash cycles can be anticipated.
Considering these scenarios, energy consumption would increase by 43% in 2020 and additional 35% in 2030. Also, water
consumption would grow by 15% in 2020 and additional 18% by 2030, not to mention the produced waste in form of, e.g.
greenhouse gas. The application of new classes of robust enzymes in the production of consumer products for relevant
industries, such as Unilever will drive a change of personal habits. In this context, Unilever has initiated a sustainable living
plan, which sets out to decouple the company's growth from the environmental impact, while at the same time increasing
Unilever's social impact. There are 3 big goals to achieve here: (i) to improve health and well-being; (ii) reduce the
environmental impact and (iii) source 100% of raw materials sustainably. The introduction of sustainable processes using
new biocatalysts produced in this project for household (FMCG) and health/cosmetic products will provide a new
awareness of the general public for the advantages to the development of an 'environmentally friendly' economy.
As described in the proposal submitted to innovate UK
The academic beneficiaries of this research would be to provide general information in enzymology. It will help to identify
new classes of enzymes that can break down components of human sebum and perhydrolases that can act as bleaching
agents. It will allow a greater understanding of enzyme mechanism and features that are responsible for protein stabllity.
The enzymes discovered will be interesting in the fact that many will originate from metagenomes so this will represent
enzymes from organisms that currently cannot be cultured. It will provide information into the so called 'dark matter' of life.
It is estimated that we can only cultivate 1-2 percent of the microorganisms in the environment to date. The new enzymes
can contribute to our general knowledge of evolution.
The enzymes discovered will have potential industrial applications in other areas of biotechnology.
As described
The development of new enzymes for fabric cleaning will benefit the general public as a whole. It will improve health and
overall quality of life. It will contribute to a sustainable economy.
As the world population steadily increases - from 7 billion in 2011 to forecasted 9 billion in 2030 (www.un.org) with people
migrating to the cities, the demand for water and other resources will multiply. By 2020, it is assumed that 1.4 billion people
will be living in urban areas, that is, 40% more than today. This strongly increases the risk for human hygiene and health
and severely impacts on the ecological and environmental balance. In this context, it is expected that more cleaning
products will be needed, for either hand or automated cleaning systems, which require more energy and more water while
producing more waste using the currently established cleaning techniques/methods. Also, higher living standards will
increase the demand for related products, notably laundry/cleaning products, per household and year, yielding more wash
cycles done per household and year. As a result, more frequent usage of warm wash cycles can be anticipated.
Considering these scenarios, energy consumption would increase by 43% in 2020 and additional 35% in 2030. Also, water
consumption would grow by 15% in 2020 and additional 18% by 2030, not to mention the produced waste in form of, e.g.
greenhouse gas. The application of new classes of robust enzymes in the production of consumer products for relevant
industries, such as Unilever will drive a change of personal habits. In this context, Unilever has initiated a sustainable living
plan, which sets out to decouple the company's growth from the environmental impact, while at the same time increasing
Unilever's social impact. There are 3 big goals to achieve here: (i) to improve health and well-being; (ii) reduce the
environmental impact and (iii) source 100% of raw materials sustainably. The introduction of sustainable processes using
new biocatalysts produced in this project for household (FMCG) and health/cosmetic products will provide a new
awareness of the general public for the advantages to the development of an 'environmentally friendly' economy.
As described in the proposal submitted to innovate UK
The academic beneficiaries of this research would be to provide general information in enzymology. It will help to identify
new classes of enzymes that can break down components of human sebum and perhydrolases that can act as bleaching
agents. It will allow a greater understanding of enzyme mechanism and features that are responsible for protein stabllity.
The enzymes discovered will be interesting in the fact that many will originate from metagenomes so this will represent
enzymes from organisms that currently cannot be cultured. It will provide information into the so called 'dark matter' of life.
It is estimated that we can only cultivate 1-2 percent of the microorganisms in the environment to date. The new enzymes
can contribute to our general knowledge of evolution.
The enzymes discovered will have potential industrial applications in other areas of biotechnology.
As described
Publications
De Rose S
(2017)
Stabilization of a Lipolytic Enzyme for Commercial Application
in Catalysts
Littlechild J
(2017)
Improving the 'tool box' for robust industrial enzymes
in Journal of Industrial Microbiology and Biotechnology
O'Halloran, S
(2022)
Stabilisation of cold-adapted lipases and their consumer-product applications
Samuel O'Halloran
(2022)
Stabilisation of cold-adapted lipases and their consumer-product applications
Description | New enzyme classes that are not currently used in commercial detergents have been identified in know genomes and metagenomes. These have been are cloned and over-expressed. purified and biochemically characterised regarding their ability to degrade components of human sebum which are not currently targeted in detergent products. Several of the novel enzymes have been shown to be more effective than the commercial enzymes currently used by Unilever.5 different Patents were filed in October 2019 for enzymes discovered in this project. One of these is now the subject of a further Innovate UK grant with Unilever and the University of Liverpool from Nov 2018 to Sep 2020. The enzymes have interesting properties in their own right and can be applied to other industrial applications. Several of the enzymes do not have any related structures deposited in the Protein Data Bank so more academic studies have commenced to determine their crystallographic structure to understand their enzymatic mechanisms and allow rational design for commercial applications. The one enzyme SHC which has been selected to be taken forward in the further grant with Innovate UK has now been crystallised for structural solution. The crystals diffract but need further improvement to change the crystal form before improved data can be collected to allow structural solution. Ongoing work is also being achieved by a BBSRC DIP Case studentship with Unilever and Exeter from Oct 2018 until September 2022,. |
Exploitation Route | The use of these new stable enzymes can be used in other industrial biotechnology applications. At least 5 of the new enzymes are of sufficient interest to be patented by Unilever to address the breakdown of human sebum in new detergent products. |
Sectors | Chemicals Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
Description | The newly identified enzymes have been cloned and over-expressed in a soluble form and have been purified and characterised biochemically. Samples of the enzymes have been supplied to Unilever for testing in assays developed at Exeter and Unilever for digestion against components of human sebum. They have also been assessed in small scale wash studies and have been shown to be more effective than the commercial enzymes currently used by Unilever. These results have resulted in another 5 patents between Exeter and Unilever which were filed in October 2019. An additional patent is currently being filed by Unilever on use of a new class of lipase enzyme which is cold active and has potential to be used for improved cleaning in ambient temperatures. One enzyme type that can breakdown squalene, which is a component of human sebum, is being taken forward for larger scale production and further assessment for industrial applications as part of a additional grant between University Exeter, University of Liverpool and Unilever from Innovate UK started Oct 2018 -Sep 2021.This has resulted in a selection of new squalene hopene cyclase enzymes that are more water soluble and easier to express at scale. These new enzymes have been studied biochemically and structurally at University of Exeter and further wash studies are continuing at Unilever. The enzymes belong to a new family of soluble squalene cyclases that have been identified that lack the membrane binding region of the more traditionally studied enzymes such as Acidiobacillus squalene hopene cyclase that can only be over-expressed in the Escherichia coli cell membrane. Two of the membrane free soluble squalene hopene cyclases have been crystallised and their high resolution structures have been determined. A publication on this work will be published as soon as any patents are accessed by Unilever. Two further studentship Training Grants, Industrial CASE CSV 2019 and 2020 start on Development of Novel Robust Laundry Enzymes for Cold Wash Applications and Using a Droplet Microfluidics enabled Approach to Protein Engineering for the detergent industry which is involving cholesterol esterases and some of the other patented enzymes from this initial BBSRC grant. In addition new lysophospholipase enzymes have been cloned an over-expressed from both a thermophilic archaeal source and a homologue of this enzyme from a bioinformatic search in the DNA database stored in Exeter. In addition a new BBSRC Industrial case studentship to study the application of lactonase enzymes for biofilm control in healthcare products has started in January 2022 with Unilever. |
First Year Of Impact | 2018 |
Sector | Chemicals,Creative Economy,Energy,Healthcare,Manufacturing, including Industrial Biotechology |
Impact Types | Societal Economic |
Description | BBSRC DTP CASE Novel Extremophilic hydrolytic enzyme for consumer product application including its extremophilic expression platform development |
Amount | £75,108 (GBP) |
Funding ID | 680062274 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2018 |
End | 08/2023 |
Description | BBSRC Training Grant, Industrial CASE CSV Development of Novel Enzymes for Improvement in Home Care Products and Fragrances |
Amount | £117,191 (GBP) |
Funding ID | BB/Y512539/1 |
Organisation | Unilever |
Sector | Private |
Country | United Kingdom |
Start | 11/2023 |
End | 09/2027 |
Description | Development of Novel Robust Laundry Enzymes for Cold Wash Applications via a Droplet Microfluidics enabled Approach to Protein Engineering |
Amount | £102,026 (GBP) |
Funding ID | BB/V509462/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2020 |
End | 09/2024 |
Description | Industrial Contribution from Unilever |
Amount | £10,000 (GBP) |
Organisation | Unilever |
Department | Unilever UK R&D Centre Port Sunlight |
Sector | Private |
Country | United Kingdom |
Start | 07/2016 |
End | 08/2018 |
Description | Industrial funding from Unilever to support a PhD studentship for Simone DeRose |
Amount | £60,000 (GBP) |
Organisation | Unilever |
Department | Unilever UK R&D Centre Port Sunlight |
Sector | Private |
Country | United Kingdom |
Start | 11/2013 |
End | 12/2017 |
Description | Novel extremophilic enzymes for new applications in Healthcare Products |
Amount | £103,929 (GBP) |
Funding ID | BB/W510476/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2022 |
End | 12/2025 |
Description | Synergy of novel chemistry and novel Biology to Combat unmet cleaning needs |
Amount | £690,556 (GBP) |
Funding ID | Innovate UK 104457 |
Organisation | Innovate UK |
Sector | Public |
Country | United Kingdom |
Start | 11/2018 |
End | 05/2021 |
Description | Collaboration with Unilever as part of Catalyst Grant |
Organisation | Unilever |
Department | Unilever UK R&D Centre Port Sunlight |
Country | United Kingdom |
Sector | Private |
PI Contribution | Unilever Industrial collaboration funded 50% by Innovate UK A newly appointed scientist in Unilever will be establishing an authority recognised standard body soiling model, preparing formulations and conducting wash studies with the newly identified enzymes from Exeter (up to medium scale) at Unilever, either in their high-throughput facility or in semi-automated single pot devices. The Exeter Biocatalysis Centre will be involved with bioinformatic identification of new novel enzymes and their cloning and over-expression allowing their biochemical characterisation and potential substrate specificity with regards to industrial applications. This will be complemented by structural studies, molecular modelling and site-directed mutagenesis which will provide complementary academic knowledge on enzyme mechanism and stability issues. |
Collaborator Contribution | Unilever will collaborate with industrial aspects of the project regarding applications of newly identified enzymes towards improved cleaning properties. Newly identified enzymes will be analysed for their ability to use substrate components of human sebum and their stability in surfactants and other components present in the commercial detergent product. |
Impact | The project kick-off meeting was in September 2016, followed by a meeting with the Innovate UK monitoring officer in Dec 2016 at Unilever, Port Sunlight. Regular meetings occur every two weeks regarding BBSRC supported Unilever PhD studentships Enzymes have been identified by bioinformatic analysis, cloning and overexpression is continuing and assays and Mass Spec analysis of activity to analyse substrate specificity is being developed. The project is multidisciplinary. Chemistry, Biochemistry, Biophysics Unilever have jointly patented several of the enzymes resulting from this project and one of the enzymes is still being evaluated for improved wash studies.omplementary academic knowledge on eThe project kick-off meeting was in September 2016, followed by a meeting with the Innovate UK monitoring officer in Dec 2016 at Unilever, Port Sunlight. The next meeting will be in Exeter on the 22nd March 2017.Further meetings between Unilever every 2 months and regarding other BBSRC studentships with Unilever established. Several joint patents have been filed from this earlier grant - see below 20-Nov-2018 C30305 WO2020104159 Lipase - hydrolase type enzyme in cleaning compositions (E.C. 3.1.1.3) Jens Carlo BENNINGHOFF, Simone Antonio DE ROSE, Michail ISUPOV, Dietmar Andreas LANG, Jennifer Ann LITTLECHILD-BOND, Sarah Rebecca SMITH, Mark Lawrence THOMPSON 20-Nov-2018 C30306 WO2020104157 Esterase type enzyme in cleaning compositions (E.C. 3.1.1.1) Jens Carlo BENNINGHOFF, Simone Antonio DE ROSE, Michail ISUPOV, Dietmar Andreas LANG, Jennifer Ann LITTLECHILD-BOND, Sarah Rebecca SMITH, Mark Lawrence THOMPSON 20-Nov-2018 C30307 WO2020104155 Isomerase type enzyme in cleaning compositions (E.C. 5) Jens Carlo BENNINGHOFF, Simone Antonio DE ROSE, Michail ISUPOV, Dietmar Andreas LANG, Jennifer Ann LITTLECHILD-BOND, Sarah Rebecca SMITH, Mark Lawrence THOMPSON 20-Nov-2018 C30308 WO2020104158 Steryl ester acyl hydrolase type enzyme in cleaning compositions (E.C. 3.1.1.13) Jens Carlo BENNINGHOFF, Simone Antonio DE ROSE, Michail ISUPOV, Dietmar Andreas LANG, Jennifer Ann LITTLECHILD-BOND, Sarah Rebecca SMITH, Mark Lawrence THOMPSON 20-Nov-2018 C30309 WO2020104156 Amidohydrolase type enzyme in cleaning compositions (E.C. 3.5) Jens Carlo BENNINGHOFF, Simone Antonio DE ROSE, Michail ISUPOV, Dietmar Andreas LANG, Jennifer Ann LITTLECHILD-BOND, Sarah Rebecca SMITH, Mark Lawrence THOMPSON One enzyme identified from this grant is still being investigated and a patent is pending.nzyme mechanism and stability issues. |
Start Year | 2016 |
Title | Amidohydrolase type enzyme in cleaning compositions |
Description | Novel thermophilic amidohydrolase enzyme for applications in detergent industry |
IP Reference | C30309 |
Protection | Patent application published |
Year Protection Granted | 2019 |
Licensed | No |
Impact | Filed Oct 2019 |
Title | Cold-adapted lipases and their consumer-product applications Submitted |
Description | Cold active lipase enzymes which have applications in healthcare applications derived from genomes of psychrophilic bacteria New class of lipase enzyme with histidine of catalytic triad of the active site located on a different element of the protein secondary structure. |
IP Reference | P0000280EP |
Protection | Patent / Patent application |
Year Protection Granted | 2024 |
Licensed | No |
Impact | Applications for cold wash applications to save energy and important for overall sustainability |
Title | DETERGENT COMPOSITIONS WITH LIPASE AND BIOSURFACTANT |
Description | Compositions comprising lipases and biosurfactants, especially psychrophilic lipases and biosurfactants. |
IP Reference | WO2017036902 |
Protection | Patent granted |
Year Protection Granted | 2017 |
Licensed | No |
Impact | Not at the moment but being incorporated into new detergent formulation at Unilever. Using cold sensitive enzyme for washing at reduced temperature for energy conservation. Sustainable use of enzymes rather than chemicals. |
Title | Esterase type enzyme in cleaning compositions |
Description | Novel thermophilic esterase enzymes for application for detergent industry with Unilevr |
IP Reference | C30306 |
Protection | Patent application published |
Year Protection Granted | 2019 |
Licensed | No |
Impact | Filed October 2019 |
Title | IMPROVED WASH COMPOSITIONS |
Description | Laundry compositions comprising lipases from Psychromonas ingrahamii. Uses of such compositions in methods of laundry, especially at low temperatures. |
IP Reference | WO2017036901 |
Protection | Patent granted |
Year Protection Granted | 2017 |
Licensed | No |
Impact | None to date but used by Unilever for new washing detergents Long term impact on enabling washing at lower temperatures thereby saving energy. Also more sustainable by using enzymes rather than chemical components in wash powders. |
Title | Isomerase type enzyme in cleaning compositions |
Description | Novel thermophilic isomerase enzyme with applications in detergent industry with Unilever |
IP Reference | C30307 |
Protection | Patent application published |
Year Protection Granted | 2019 |
Licensed | No |
Impact | Filed October 2019 |
Title | LIQUID DETERGENCY COMPOSITION COMPRISING LIPASE AND PROTEASE |
Description | A liquid detergency composition comprising a protease and a lipase, wherein the lipase comprises a polypeptide having an amino acid sequence which has at least 90 percent sequence identity with the wild-type lipase derived from Humicola lanuginosa strain DSM 4109 and, compared to said wild-type lipase, comprises a substitution of an electrically neutral or negatively charged amino acid within 15 A of E1 or Q249 with a positively charged amino acid. |
IP Reference | WO2017036917 |
Protection | Patent application published |
Year Protection Granted | 2017 |
Licensed | No |
Impact | None to date. Will be used for new detergent formulations by Unilever |
Title | LIQUID DETERGENCY COMPOSITION COMPRISING PROTEASE AND NON-PROTEASE ENZYME |
Description | A liquid detergency composition comprising: protease and non-protease enzyme wherein at least part of either the protease or the non-protease enzyme is cross- linked enzyme aggregate. |
IP Reference | WO2017036915 |
Protection | Patent granted |
Year Protection Granted | 2017 |
Licensed | No |
Impact | Not at the moment but being incorporated in new liquid detergent design by Unilever |
Title | Lipase - hydrolase type enzyme in cleaning compositions |
Description | Novel thermophilic hydrolase enzymes EC 3.1.1,3 with potential application for domestic detergent products |
IP Reference | C30305 |
Protection | Patent application published |
Year Protection Granted | 2019 |
Licensed | No |
Impact | Filed in October 2019 |
Title | PROCESS TO MANUFACTURE CROSS-LINKED ENZYME AGGREGATES |
Description | A process for the manufacture of cross-linked enzyme aggregates comprising the steps of: 1) providing a liquid mixture of the enzyme; 2) precipitating the enzyme comprised by the liquid mixture; 3) cross-linking the enzyme by addition of a cross-linking agent, having at least two reactive groups suitable for cross-linking amino acids; 4) stopping the cross-linking by addition of a suitable buffer; wherein step 2) is performed in the presence of a compound selected from a crown ether or a surfactant; and wherein step 4) is preferably performed when the mean diameter of the particles is at most 4.0 µm, as measured using a Turbiscan MA200 at 21 degrees Celsius, using the Stokes-Einstein equation to measure the particle size. |
IP Reference | WO2017036916 |
Protection | Patent granted |
Year Protection Granted | 2017 |
Licensed | No |
Impact | Not at present but being developed by Unilever for new detergent formulation |
Title | Steryl ester acyl hydrolase type enzyme in cleaning compositions |
Description | Novel enzyme steryl ester acyl hydrolase with applications in detergent industry |
IP Reference | C30308 |
Protection | Patent application published |
Year Protection Granted | 2019 |
Licensed | No |
Impact | Filed October 2019 |
Description | BBSRC BIOCATNET meeting, London, 2014 Oral presentation |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Study participants or study members |
Results and Impact | Novel Thermostable Transfer Enzymes for Industrial Biocatalysis. |
Year(s) Of Engagement Activity | 2014 |
Description | Plenary Lecture, European Congress on Biotechnology, ECB16 Krakow, Poland, 3rd to 6th July, 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Dissemination of results of THERMOGENE to academics, industry and policy makers |
Year(s) Of Engagement Activity | 2016 |
Description | Poster Presentation by PhD student Josh Tierney |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Industry/Business |
Results and Impact | Unilever PhD conference Colworth Park Novel Extremophilic Hydrolases for Biofilm Disruption |
Year(s) Of Engagement Activity | 2022 |
Description | Poster Presented at Novel Enzymes Conference in Germany by PhD student Hayley Blaber |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Novel enzyme development for healthcare products and evolution using microfluidic techniques for industrial requirements and operation conditions |
Year(s) Of Engagement Activity | 2023 |
Description | Poster at 5th International Conference on Novel Enzymes, Groningen, the Netherlands |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Poster presentation: Stabilisation of a lipolytic enzyme for Commercial Applications, Simone Antonio De Rose and Jennifer Littlechild |
Year(s) Of Engagement Activity | 2016 |
Description | Poster at International Extremophile Meeting by PhD student on Cold Active Enzymes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Dissemination to an International audience of applications and development of cold active enzymes for detergent applications and sustainability issues by energy saving with cold washing. Enzyme stabilisation in detergent additives. Poster Title -Stabilisation of a novel psychrophilic enzyme |
Year(s) Of Engagement Activity | 2023 |
Description | Poster at International Protein Stabilisation Meeting, Bulgaria 2021 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Poster Title - Enzymes at the extreme: Life in the hot and cold PhD student Unilever and BBSRC Samuel O'Halloran |
Year(s) Of Engagement Activity | 2021 |