Brassica Rapeseed And Vegetable Optimisation

Lead Research Organisation: John Innes Centre
Department Name: Crop Genetics

Abstract

Agriculture is facing the crucial challenge of adapting crop productivity to changes in the climate. More variable weather patterns require the development of crops that are able to perform more robustly under a wider range of environmental conditions. At the same time, climate change also provides new opportunities for increasing the length of the UK vegetable growing season and increasing food security by reducing imports of fresh produce during the winter, but this requires breeding new varieties that are able to produce robustly at different times of year.

The BRAVO consortium aims to meet these challenges through close interactions between academia and industry. To achieve this goal, we have brought together world-leading experts in both Arabidopsis and Brassica plant reproduction from research institutes and universities within the UK. As the result of a series of meetings between consortium members and stakeholders from the oilseed rape and vegetable Brassica industries, optimisation of flowering and coordination of developmental transitions in the production of high-quality seeds were identified as important common targets. These transitions that occur during plant reproduction such as to flowering, fertilisation, inflorescence growth, seed production, dispersal, and subsequent seed performance are now known to be managed by environmentally responsive gene networks built on a foundation of common components first described for their ability to control flowering time.

The goal of BRAVO is to provide a mechanistic understanding of the role of flowering time gene networks in the control of Brassica reproductive developmental transitions from vegetative growth through to seed production and seed vigour. Because these networks control environmental responsiveness, this knowledge can be exploited to increase robustness in the performance of oilseed and vegetable Brassicas. A key challenge is how to optimise individual traits when the same flowering time gene network has been optimised by evolution over millions of years for multiple functions, each of which is important for crop performance. In this proposal, we will combine genomics and phenomics technologies with approaches in developmental genetics and mathematical modelling to link genotype to phenotype for master regulators of key transitions during Brassica reproductive development. Through exploitation of available genetic resources, we will reveal the architecture of flowering time gene networks in Brassicas and how they have been modified in the past by plant breeders to cause trait variation, life history variation and climate adaptation. This will allow us to develop a predictive framework for designing strategies to vary specific crop characteristics without harming others, and to generate and test novel genetic variation with potential uses in future trait enhancement.

In parallel we will establish and exploit resources such as a gene expression atlas and targeted gene disruption which will allow the Brassica research and breeding communities to expand knowledge on important biological processes and use the outputs form BRAVO collectively to improve Brassica crop performance.

Long-term improved and sustainable Brassica crop performance can only be achieved through fundamental understanding of biological processes. The composition of the BRAVO consortium allows the combination of excellence in Brassica research with knowledge transfer from the closely related Arabidopsis model species. The project builds on and expands academia-industry interactions through industrial membership on the project's Supervisory Board, industry engagement and practical involvement in case studies, frequent consortium meetings and annual stakeholder events.

We believe this project provides a unique opportunity to align industry priorities with excellent fundamental research programmes in order to help secure the future yield of Brassica crops in the UK and worldwide.

Technical Summary

A fundamental challenge in modern crop science is the exploitation of big genomic data by linking variation in genotype to phenotype. Flowering time pathways are now recognised as master regulators of plant development, and as signal-processing machineries allowing plants to adapt to their environment. These genes affect all major reproductive processes from the timing of flowering, through inflorescence architecture, flower, fruit and seed development and finally seed vigour. The goal of BRAVO is to combine genomic tools, predictive biology and high throughput phenotyping to efficiently link genotype to phenotype across multiple reproductive traits sharing regulatory components from flowering time pathways in Brassica napus and B. oleracea. We hypothesise that investigating each transition as part of an integrated project could target breeding programmes towards loci offering the opportunity for a win-win: that is to positively manipulate more than one trait, and to predict inevitable trade-offs between traits that maximise yield in different environments.

High-throughput phenotyping will be used for association genetics approaches in both B. napus and B. oleracea to identify candidate genes linked to trait variation in variety panels. In parallel, construction and validation of in silico gene networks will be combined with large-scale EcoTILLING to make phenotypic predictions for each of ~200 Brassica varieties based on the ability of alleles to disrupt network architecture and critical outputs for each trait. Candidate genes controlling each trait and their place in the network will be validated in Brassica by exploiting our TILLING and transformation platforms together with our novel CRISPR pipeline at JIC.

Close integration of our research programme with industrial stakeholders will see germplasm with commercial potential taken into field trials, providing a seamless innovation pathway from laboratory to field.

Planned Impact

Oilseed rape is the second most important combinable crop in the UK with an annual market value in excess of £800M with the vegetable Brassica crop worth a further £227M (Food and Agricultural Organisation of the United Nations, FAOSTAT, 2014; Defra statistics, 2014). Varying environmental conditions often result in unpredictable heading, fertility, seed yield and seed quality resulting in estimated losses in excess of £30M per annum through poor establishment, up to £40M from unscheduled harvest and an estimated 20% loss (£160M in 2014) from pod shatter resulting partially from asynchronous development. The commercial seed industry also experiences significant losses through poor seed batch performance thus making production of high-yielding varieties with quality seed both as a commodity and for commercial seed multiplication a key target for the Brassica industry.

This project will address the delivery of more robust oilseed and vegetable Brassicas to combat these losses by targeting five, industry validated, fundamental concerns: (1) uniform and shortened flowering period through more determinate flowering, (2) fertility under changing environments, (3) reduced seed loss through infertility, pod shatter and pre-harvest sprouting, (4) properties of the seed coat for downstream processing and (5) the homogeneity and speed of seed development and germination. A greater understanding of the genetic control underlying these traits in current varieties and identifying allelic variation underlying phenotypic differences will have a large impact on the breeding of improved varieties to suit the UK market and help the oilseed and Brassica industry maintain and enhance its current competitive advantage.

The project targets two major BBSRC Agriculture and Food Security strategic priority areas: Priority 4: Reducing and re-using waste and Priority 5: understanding "genotype X environment = phenotype". The combined expertise of the academic partners together with thedustrial collaborators will develop a strategic pipeline to optimise brassica productivity through understanding of the key developmental transitions and trade-offs during plant growth.

Specific impacts will take the form of:

1. Reducing industry waste and crop losses due to unscheduled harvest and yield and quality reduction through asynchronous pod development by understanding the factors controlling development.
2. Help ensure future crop supply in a changing environment by understanding environmentally sensitive developmental traits and how to genetically mitigate effects in future climate scenarios.
3. The introduction of new knowledge, technologies, tools and capabilities to the private sector to assist in crop breeding and management (i.e. associative transcriptomic pipeline for vegetable Brassicas, gene expression browser for accelerated gene discovery, database of allelic variation to facilitate marker assisted selection, CRISPR technologies). Through direct industry participation, shape further research development to best support industry.
4. Produce valuable new germplasm with desirable traits, associated markers and seeds, and work with project partners to assess their suitability for product development pipelines. Develop mechanisms for delivery and utilisation of outputs to foster continuous opportunity and commercial exploitation in industry.
5. Introduce and train young scientists and researchers in crop genomics and breeding as a career path to ensure quality future researchers in both public and private sector.
Nurture relationships between academia and industry stakeholders across the supply chain, enhancing understanding and interaction between research and industry thus increasing support for future collaboration.

Publications

10 25 50

 
Description A key output from BRAVO will be an expression atlas providing detailed information of gene expression patterns at a genome-wide level throughout development of B. napus and B. oleracea. To this end, we have collected tissue material from four different B. napus varieties and two different B. oleracea varieties at a range of developmental stages from vegetative tissue over apices to individual and finely dissected reproductive tissues. Global gene expression data have been obtained in B. napus varieties for several developmental stages and in different tissues by partners at JIC, RRes and UoN. These transcriptomes are currently being modelled to identify specific genetic regulatory modules involved in mediating developmental transitions. Publicly available Arabidopsis developmental transcriptome data are being used to develop comparative modelling approaches between model and crop and determine potential genetic sub-functionalisation. Tissue has been obtained for B. oleracea varieties as well and RNA will be sent for RNAseq shortly. Experimentation to look at gene expression under environmental perturbation is currently underway to study gene x environment effects.

Combining expression data with phenotypic traits is pivotal to the work in all themes of the BRAVO programme. Therefore, efficient high-throughput phenotyping is essential in BRAVO and we have run a B. napus diversity set at the phenotyping platform at UoA. Software has been developed to extract specific features such as yellowness. This allows us to automate flowering time assessment and immediately extract such data from the experiment. These data are being supplemented with large scale phenotyping trials at UoN, RRes and John Innes. UoN have developed high throughput pollen viability scoring which is currently being used to assess male fertility of the B. napus diversity set under standard and stress conditions. RRes in collaboration with UoA have phenotyped the same population for female fertility and productivity traits. Phenotyping at JIC has provided germination and flowering time data and an extensive nested trial to look at vernalisation and maturation environments on developmental traits and productivity is in progress. Combined, these data provides a comprehensive phenotypic dataset to dissect the effect of environment on phenotype and, ultimately, crop success. The use of the associative transcriptomics pipeline from the UoY has already identified candidate genes for potential further studies.

We encountered problems amplifying seeds from many of the lines in our B. oleracea diversity set. Syngenta has representation on our management board with Brassica vegetable breeder, Frank Lanfermeijer, and his team has grown the B. oleracea lines on a Syngenta location in Kenya. They have managed to obtain seeds from most lines that will allow us to carry out the proposed work. An initial round of multiplication by Syngenta has allowed the large-scale phenotyping of the B. oleracea diversity set for developmental response to vernalisation temperature and length at JIC. These data are essential for the effective use of the population for further studies and therefore add considerable value to the diversity set. The UoY have developed an associative transcriptomics pipeline for genome-wide association analysis within this species. Seed fatty acid data is being generated to allow QA testing of this pipeline and initial analysis of the phenotyping data available has identified potential candidates of interest. Work by the UoW has determined the key timepoint for the juvenile-to-adult developmental transition.

In the next few months, we will focus on deciding on the best approach to make a public expression atlas server that will allow the community to visualise the expression pattern of their genes of interest. Meetings have been held with colleagues in the Computational and Systems Biology department at the John Innes Centre as well as with colleagues at the EBI to link our expression data with the international EnsemblPlants genome browser. We aim to present the outcome at the Plant and Animal Genomes Conference in San Diego in January 2020.
Exploitation Route The aim of BRAVO is to unravel mechanisms that control developmental transitions during the growth of Brassica crops with a focus on oilseed rape (B. napus) and vegetable brassicas (B. oleracea). We do this via genome-wide expression analyses coupled with phenotypic analysis of large diversity panels and mathematical modelling. As a direct output from BRAVO, we will create a public expression atlas for the community to exploit.
Our progress so far involves coordination of experimental work and a close and continuous dialogue with stakeholders. These activities have and will continue to foster new collaborations and interactions across disciplines (e.g. between experimentalists and computer scientists). In fact, in the last year we have initiated a collaboration between BRAVO and mathematician Dr. Daphne Ezer from the Alan Turing Institute. They have developed software to analyse expression data and identify genetic networks involved in plant development. In this project, the Ezer group will analyse our data in their pipeline and they have been successful in obtaining funding for a 12-month posted position to do so from within ATI. The postdoc participated in our most recent stakeholder meeting.
In addition, several members of the BRAVO team participated and were invited speakers at the Brassica 2018 conference in St Malo. The PI was part of the scientific organising committee and several new links were formed at that meeting.
BRAVO team members participated in the two meetings of Multinational Brassica Genome Project consortium - one at the meeting in St Malo and another at the PAG conference in San Diego in Jan 2019. These meetings are excellent vehicles to communicate progress in the BRAVO project and follow any development at the international Brassica stage.
As an exciting new development, Judith Irwin (co-investigator on BRAVO) obtained an international partnering award from BBSRC to establish links with colleagues in Australia. Most of the JIC BRAVO team went for a symposium at Southern Cross University in Australia in Sep 2018 with a reciprocal symposium taking place at JIC in March 2019. Discussions are ongoing on how to further coordinate work and continue this collaborative initiative beyond the life-time of BRAVO. In particular, the environmental aspect of BRAVO would be interesting to pursue and test in a very different climate with different varieties.
Sectors Agriculture, Food and Drink,Environment

 
Description Despite the fundamental nature of the experimental programme, it is of utmost importance for the success of BRAVO that we maintain close interactions with stakeholders including the breeding industry, farmers and the public. In particular, we are grateful for the excellent dialogue and interactions we have with participants at our stakeholder and advisory committee meetings. These interactions have given us an essential steer and ensures that our findings will be picked up by industry as they emerge. Regarding traits, our work on pod shatter will continue within BRAVO. Through meetings with the Agriculture and Horticultural Development Board (AHDB), it is clear that there is an increasing interest in including pod shatter assessment of oilseed rape varieties that are on the UK Recommended List. This assessment will be through the use of the Random Impact Test system and the newly developed software associated with the analysis of data, both of which were developed at the JIC. This would be a fantastic outcome in terms of impact and discussions on how best to facilitate this are ongoing between AHDB and JIC. In the past year, members of the BRAVO consortium have participated in a number of outreach and stakeholder-interaction activities all of which are included in the Engagements section of ResearchFish. Of particular highlights are articles from BRAVO team members in the farming press and national press, interviews on national radio, hosting farmers and breeders visiting institutes, school visits and presentations on the BRAVO project at AgriTechEast events including Cereals 2018. While we learn an immense amount about our own projects through these activities, they also contribute to making non-academics aware of the importance of science for addressing some of the biggest challenges facing the world's population today in terms of climate change and food security. There is no doubt that multidisciplinary, publicly-funded, scientific consortia such as BRAVO will be required to overcome these challenges to the benefit of society.
First Year Of Impact 2018
Sector Agriculture, Food and Drink,Environment
Impact Types Societal

 
Description Invited participant to discuss UK participation on the EMPHASIS initiative to coordinate phonemics platforms across Europe
Geographic Reach Europe 
Policy Influence Type Participation in a national consultation
 
Description GCRF Foundation Awards for Global Agriculture and Food Systems Research - 'Genetic improvement of rice seed vigour for dry direct-seeded conditions'
Amount £650,000 (GBP)
Funding ID BB/P023428/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2017 
End 04/2018
 
Description GCRF Impact Acceration Award- Comparative transcriptomic analysis of mesocotyl elongation in rice
Amount £38,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2017 
End 03/2017
 
Description Global Challenges Research Fund - Impact Acceleration Award Extension 2017 - 'Development and validation of new open-source phenotyping platforms for analysis of rice seedling vigour'
Amount £5,740 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 12/2017 
End 02/2018
 
Description NASPA
Amount € 278,264 (EUR)
Funding ID NASPA 
Organisation European Commission 
Department Interreg : European Territorial Co-operation
Sector Public
Country European Union (EU)
Start 01/2018 
End 01/2021
 
Description Project with Brandon Biosciences Ltd to test the effect of seaweed extracts on pod shatter in oilseed rape
Amount € 170,000 (EUR)
Funding ID Project ID: 652506 
Organisation European Commission H2020 
Sector Public
Country Belgium
Start 10/2017 
End 09/2018
 
Description Strategic LoLa
Amount £4,392,784 (GBP)
Funding ID BB/P003095/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2017 
End 12/2021
 
Title Random Impact Test version 2.0 
Description The Random Impact Test is to use for assessing pod shatter resistance in oilseed rape. We have further improved the method to also include other members of the Brassicaceae family including Arabidopsis, Capsella and Lepidium. Moreover, newly developed software for curve fitting has allowed more precise and automated calculations. 
Type Of Material Biological samples 
Year Produced 2016 
Provided To Others? Yes  
Impact We have been approached by industry and academics to carry out this assay on a range of material. 
 
Description Daphne Ezer at Alan Turing Institute 
Organisation Alan Turing Institute
Country Unknown 
Sector Academic/University 
PI Contribution Collecting detailed expression profiles from a range of developmental stages and tissues of Brassica naps and Brassica oleracea.
Collaborator Contribution They have developed software to analyse expression data and identify genetic networks involved in plant development. In this project, the Ezer group will analyse our data in their pipeline and they have been successful in obtaining funding for a 12-month posted position to do so from within ATI. The postdoc participated in our most recent stakeholder meeting.
Impact Too early to list any outcomes. This is a multidisciplinary collaboration between experimentalists and mathematical modellers.
Start Year 2018
 
Description Testing seaweed extract for combatting pod shatter in oilseed rape 
Organisation Brandon Products Ltd
PI Contribution Testing different formulars of seaweed extract in reducing pod shatter of oilseed rape. Assessing whether the effect occurs via the SHP/IND-mediated pathway by testing the effect of the extracts on gene expression in Arabidopsis fruits.
Collaborator Contribution Production of seaweed extracts and further refinement to identify active compounds.
Impact Seaweed extracts have been tested on several varieties of oilseed rape as well as on reporter lines of Arabidopsis. No effect has been detected on pod shatter resistance, but it does seem in Arabidopsis as if the valve margin auxin response minimum is compromised, suggesting the extract has an effect on the programme leading to seed dispersal.
Start Year 2016
 
Title Random Impact Machine V2 
Description New version of the Random Impact Machine for pod shatter testing produced complete with upgraded software for the analysis of the results. Software is freely available on GitHub and currently submitted for publication. 
Type Of Technology New/Improved Technique/Technology 
Year Produced 2018 
Impact Machine and software have been sold commercially as a packet. The systems is currently being investigated by the AHDB as a criteria for the variety National List testing system. 
 
Description Festival of Science and Curiosity 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Festival of Science and Curiosity 2019 - 16th, 18th and 20th February 2019 - engaged with approx. 200-250 children (ages 2-9) (Feedback from 160) and approx. 100-150 parents (Feedback from 60).
Year(s) Of Engagement Activity 2019
 
Description 2nd BRAVO BBSRC sLoLA Stakeholder meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Industry dissemination meeting for the BRAVO BBSRC sLoLa to update industry stakeholders on project progress across the previous year. The meeting also offers the opportunity to gather industry feedback to shape the project and potential further research opportunities. 40 members of industry and academia attended the meeting which is deliberately organised into short presentations with longer discussion sessions to encourage interaction. Since the meeting there have been requests for further information from a number of attendees and some industry visits have been arranged.
Year(s) Of Engagement Activity 2019
 
Description Article for farming press 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Brief article written for BSPB Plant Breedig Matter magazine on hybrid breeding and farm saved seed. This will have reached a raming audience explaining variety production, the plant breeding process and breeding for important traits
Year(s) Of Engagement Activity 2019
URL http://www.bspb.co.uk/BSPB_Plant_Breeding_Matters_Spring_2018.pdf
 
Description Article published in the Times 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Article reporting development of new short generation Broccoli
Year(s) Of Engagement Activity 2017
 
Description Attendance of a genome database workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Attendanec of the Ensembl 4 Breeders workshop at EBI to discuss use of Ensembl plants database and future interactions and accesibility for data that is being generated by the BBSRC sLoLa BRAVO and the UK Brassica Research Community. The workshop included a planning session for future functionality that is desired within EnsemblPlants. This discussion has influenced the work which will be taken forward at the EBI. Potential funding opportunities were discussed. Discussion of the data generated within BRAVO highlighted the potential use to the breeding community.
Year(s) Of Engagement Activity 2019
 
Description Attendance of a genome database workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Attendence of the Ensembl 4 breeders workshop at EBI to discuss use of Ensembl plants database and future interaction and accessibility for data that is being generated by the BBSRC sLoLa BRAVO and the UK Brassica Research Community. The workshop included a planning session for future functionality that is desired within Ensembl plants. This discussion has influenced the work which will be taken forward at the EBI. Potential funding opportunities were discussed. Discussion of the data generated within BRAVO highlighted the potential use to the breeding community.
Year(s) Of Engagement Activity 2019
 
Description BBSRC Science showcase event at Camden BRI 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Industry-academia interactions in a BBSRC-organised event at Camden BRI to showcase relevant BBSRC projects. I presented the BRAVO programme.
Year(s) Of Engagement Activity 2017
 
Description BBSRC sLoLa Brassica Rapeseed and Vegetable Optimisation 1st Annual Stakeholder Meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Annual meeting with industry stakeholders for the BRAVO sLoLa. Including industry members from the breeding community, agronomy companies, farmers, NFU, AHDB, BSPB, BBSRC and academic partners. Meeting is to disseminate activities and outcomes from the BRAVO project
Year(s) Of Engagement Activity 2018
 
Description BRAVO stakeholder meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Annual BRAVO sLoLa stakeholder meetings where members of the breeding/growing industry discuss progress on the BRAVO project.
Year(s) Of Engagement Activity 2018
 
Description BRAVO stakeholder meeting 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact 2019 BRAVO stakeholder meeting for the dissemination of current research and project outcomes to the project stakeholders. Sessions are organised with short talks from the project members followed by longer discussion sessions to capture feedback on the ongoing work and direct potential work in the future.
Year(s) Of Engagement Activity 2019
 
Description Brassica science - DSV networking event 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Visit to JIC by the breeding company DSV. As a breeding company DSV have had limited engagement with JIC and the UK Brassica research community. This visit has sparked stronger interaction between organisations and potential future employment opportunities for JIC graduates.
Year(s) Of Engagement Activity 2017
 
Description Breeder's day at JIC - Presentation of Brassica research 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Talk/discussion on Brassica science at JIC involoving brassica research material. Good response from the audience , question and interactions with journalists leading to follow up talks and media interaction.
Year(s) Of Engagement Activity 2017
 
Description Building Better Brassicas - Veg Farmer 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Interview with Heather Mills, an independent journalist that works with Farmers Guardian and The Veg Famer magazines. This resulted in an article in Veg Farmer about the vegetable work being completed in the BBSRC BRAVO project.
Year(s) Of Engagement Activity 2018
URL https://www.vegetablefarmer.co.uk/
 
Description Clover Seeds and CN seeds visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Visit to JIC from members of Clover and CN Seed to discuss current industry needs and work ongoing at JIC where there could be potential interaction.
Year(s) Of Engagement Activity 2018
 
Description Combatting the Challenges of Oilseed Rape - AHDB agronomy Showcase presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Invited speaker at an AHDB event following contact made at the Hutchinsons Winter Agronomy conference. Presentation on the broader challenger facing oilseed rape in the UK, mainly around establishment and seedling vigour, part of BRAVO. Questions sparked following the talk and good discussions at the event later with obviously good agronomist interest in developments. There has been the potential for further AHDB BRAVO interactions discussed. This is one of our preferred dissemination routes.
Year(s) Of Engagement Activity 2018
 
Description Elevator pitch at Cereals 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact As part of an AgriTechEast event (Innovations Insight) at Cereals 2018, I gave a 2-min pitch about the BRAVO strategic LoLa grant on Brassica Rapeseed And Vegetable Optimisation.
Year(s) Of Engagement Activity 2018
URL https://www.agritech-east.co.uk/tag/cereals-2018/
 
Description Elsoms Veg open day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Stand at the Elsoms Vegetable open day displaying the current work of JIC. Discussed many current projects with the industry members that visited the open day and trials
Year(s) Of Engagement Activity 2018
 
Description Elsoms seeds visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Visit by Elsoms seeds to discuss Speed breeding and speed cloning of disease resistance genes . Good discussions had.
Year(s) Of Engagement Activity 2018
 
Description Elsoms seeds visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Visit regarding Brassica research at JIC. Good discussion had
Year(s) Of Engagement Activity 2018
 
Description Engagement with industry, agronomy company 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact JIC site visit with Prime agriculture, the UK's largest independent agronomy company after they approached JIC with an interest in JIC science. Work presented from the JIC Brassica program as this is an area of key importance.
Year(s) Of Engagement Activity 2018
 
Description Engagement with industry, breeding company 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Visit to JIC by CN Seeds (UK company with which we are engaged) and Clover Seeds (Chinese breeding company) after a request to KEC and interest shown in Brassica science at the John innes.
Year(s) Of Engagement Activity 2018
 
Description Engagement with industry/stakeholder 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Meeting with Elsoms on several aspects of seed/reproductive research as avenues for future collaborations.
Year(s) Of Engagement Activity 2018
 
Description First Annual Stakeholder Meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact The first Annual BRAVO stakeholder meeting was organised on the 1st of February 2018 at Rothamsted Research. Besides project partners and members of the supervisory board, over 21 stakeholder/companies were invited to the meeting. Along with project presentation, vegetable and oilseed breeders presented challenges faced by them and this was followed by several feedback and discussion sessions.
Year(s) Of Engagement Activity 2018
 
Description Flying Farmers visit to JIC 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Visit by a group of farmers based throughout the UK so they could interact with science and de3velop an understanding of the crop science taking place at JIC
Year(s) Of Engagement Activity 2017
 
Description GARNET 2018 invited talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Invited presentation of the BBSRC SLoLA BRAVO at the GARNET Arabidopsis conference in the model to crop session. Talk provoked good questions and discussions following the session.
Year(s) Of Engagement Activity 2018
 
Description Hutchinsons Winter Agronomy Conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact BRAVO information dissemination stand at the Hutchinsons Winter Agronomy Conference to provide the opportunity to interact with industry agronomists and farmers. Resulted in continues iteraction and participation from Hutchinsons with the BRAVO project.
Year(s) Of Engagement Activity 2017
 
Description Interview for Horticultural press 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Interview with Freelance journalist writing articles for Horticultural press resulting in article in the vegetable farmer
Year(s) Of Engagement Activity 2018
 
Description Interview for BBC Radio 4 farming today 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Radio interview discussimg new short generation broccoli
Year(s) Of Engagement Activity 2017
 
Description Interview for BBC Radio Norfolk Drive time 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Live radio interview discussing new short generation broccoli
Year(s) Of Engagement Activity 2017
 
Description Interview with National Public Radio, USA 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interview with journalist at National Public Radio in the US regarding publication of Molecular Plant paper on temperature-induced acceleration of pod shatter in oilseed rape.
Year(s) Of Engagement Activity 2018
 
Description Interview with eastern daily press 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact As ISP funding was publicised, local news media ran stories on the impact this will have to science, the environment, industry and the region.
Year(s) Of Engagement Activity 2017
 
Description Invited Speaker, Brassica2018, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited to give Keynote lecture at Brassica 2018 entitled: The impact of flowering time genes on crop productivity: more than when to flower
Year(s) Of Engagement Activity 2018
 
Description Invited Talk at Knowledge Transfer network event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Presentation describing how understanding the regulation of flowering time can contribute to combating the impacts of climate change in Brassica breeding
Year(s) Of Engagement Activity 2018
 
Description JIC Science Innovation Showcase 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact JIC scientists presenting their science to invited industry/companies
Year(s) Of Engagement Activity 2018
 
Description Lives in Science - Schools Arts and Science workshop with Malca Schotten 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact 15 pupils attended a school art workshop, with examples of lives in science and ongoing projects used to spark discussions about being a scientist and produce related artwork.Pupils developed a greater idea about working in science, career path, job diversity etc.
Year(s) Of Engagement Activity 2018
 
Description MSc lecture x 2 - Brassica genetics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Two lectures on Brassica genetics given for the University of East Anglia Plant Breeding MSc course. Lectures contained information on past and current work at JIC and provoked questions and discussion.
Year(s) Of Engagement Activity 2018
 
Description MSc lecture x 2 - Brassica genomics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Two lectures on Brassica genomics presented for the University of East Anglia Plant Breeding MSc course. Lectures for the teachig part of the students gaining this qualification. Lecture provoked questions and discussion from the students in this area. Ongoing work within Brassica genomics at the institute was included.
Year(s) Of Engagement Activity 2019
 
Description MSc lecture x 2 - Hybrids and heterosis 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Two lectures given on Hybrids and Heterosis for the University of East Anglia Plant Breeding MSc course. Details of past and current work at JIC included. Lecture stimulated good discussion and questions.
Year(s) Of Engagement Activity 2018
 
Description Mardlers dinner discussions 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact A group of local landowners/farmers (the Mardlers) were invited to JIC for discussions on current research. The Mardlers regularly meet in the local area with a JIC representative being in attendance at every meeting. As an alternative this event was held onsite at JIC. Discussions were held about current research at JIC and opinions heard from both the science, farming and economic areas. Both scientist and other attendees were educated about opinions and practices.
Year(s) Of Engagement Activity 2018
 
Description Morley Innovation Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Stand at Morley Innovation Day to present the current Brassica work at John Innes to the farming community and general public. Discussions held with many of the stalls visiotrs.
Year(s) Of Engagement Activity 2018
 
Description Multinational Brassica Genome Project Committee meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Participation and project dissemination at the MBGP consortium meeting. BRAVO project resources/ expression brwoser and related data to be integrated into the Multinational Brassica resources and network to coordinate development of Brassica resources worldwide.
Year(s) Of Engagement Activity 2017
 
Description One month training visit to JIC by Researcher from Bangladesh Agricultural Research Institute 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Third sector organisations
Results and Impact One Month visit by Researcher from the Oilseed Research Centre at the Bangladesh Agricultural Research Institute for training in sequence analysis, primer and marker design for use in breeding activities at BARI to help improve mustard productivity
Year(s) Of Engagement Activity 2018
 
Description Optimising Brassica genetics to meet growing challenges - Bayer Annual Potato and Vegetable Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Invited speaker at the Bayer Potato and Vegetable Conference following initial BRAVO press release http://www.fruitnet.com/fpj/article/171098/ff. Good questions from the audience and discussions following the talk.
Year(s) Of Engagement Activity 2017
 
Description Organised a meeting with Australian Collaborators 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Organised and ran a highly interactive three-day workshop to foster collaboration between UK and Australian Researchers working on on Brassica Genomics, genetics and phenology
Year(s) Of Engagement Activity 2018
 
Description Oxford Farming conference BBSRC Innovation Hub 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact BRAVO stand representing BBSRC strategic target areas at the BBSRC Innovation Hub at the Oxford farming conference. Over 110 people involved in attending the event over 2 sessions. Multiple participant chose to come and discuss the work, application and potential future directions. Interviews performed with media attending the event. Email contact was established with several event attendees.
Year(s) Of Engagement Activity 2018
 
Description Presentation at Brassica workshop, PAG 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited presentation in Brassica workshop at Plant and Animal Genome conference, 2019 entitled: Dissecting vernalisation: keeping the greens on your plate
Year(s) Of Engagement Activity 2019
 
Description Presentation at STEM event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Presentation to girls from a selection of schools at a STEM event
Year(s) Of Engagement Activity 2018
 
Description Press article for regional newspaper 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Article the the Press and Journal following press release about new short generation broccoli
Year(s) Of Engagement Activity 2017
 
Description Prime Agriculture LLP visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Visit by Prime agriculture to JIC to discuss ongoing work which may be of interest to crop consultants.
Year(s) Of Engagement Activity 2018
 
Description Radio interview- Radio 4 Farming Today 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Interview in the glasshouse among Brassica plants with Anna Hill from BBC Radio 4 Farming Today programme. Specifically describing the BRAVO programme with clear connections and links to the GEN ISP.
Year(s) Of Engagement Activity 2017
 
Description Recommended list board talk 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Visit to present work on Random Impact testing to the Recommended List Board for oilseeds. Good disccussion followed and furhter work has now been completed in this area.
Year(s) Of Engagement Activity 2018
 
Description Seminar at Institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave Seminar at CSIRO, Black Mountain, Canberra
Year(s) Of Engagement Activity 2018
 
Description Talk at Annual Stakeholder meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Annual BRAVO sLoLa stakeholder meetings where members of the breeding/growing industry discuss progress on the BRAVO project.
Year(s) Of Engagement Activity 2018
 
Description Talk at Eastern Agritech event 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Talk to a business audience on developing germplasm for 21st Century Horticultural production
Year(s) Of Engagement Activity 2017
 
Description Talk at Farm Manager conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact The Director, Dr Achim Dobermann presented the work being carried out in BRAVO, and WP4 to the attendees at the Velcourt farm Manager's Conference on 12th January 2018.
Year(s) Of Engagement Activity 2018
 
Description Talk at OREGIN meeting, York, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Presentation to breeders and academia within the UK Oilseed RapE Genetic Improvement Network
Year(s) Of Engagement Activity 2017
 
Description Talk at Vegetable consultants conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Talk and Q&A session to group of Vegetable consultants who inform framers, growers and producers
Year(s) Of Engagement Activity 2017
 
Description The Cut: Science Cafe 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Invited talk at a local science cafe followed by panel debate and discussion session. Talk on Building Brilliant Brassicas ranged from researching current targets for crop improvement to genome editing. Good discussion, feedback and debate was received.
Year(s) Of Engagement Activity 2018
 
Description Visit to INRA in Caen 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Meetings , discussions with CSIRO partners on the most complementary approach between our organisations for future studies of phenology. I gave a Seminar to the INRA research group
Year(s) Of Engagement Activity 2017
 
Description Visit from Bayer Crop Science 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Discussions on SeedGerm - tracking and assessing seed germination
Year(s) Of Engagement Activity 2018
 
Description Visit from Collaborator, Southern cross university 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Discussions and a seminar from SCU visitor on future collaborations
Year(s) Of Engagement Activity 2017
 
Description Visit to INRA Rennes 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Seminar and discussion on joint collaborations and future activity
Year(s) Of Engagement Activity 2017
 
Description Visit to JIC by the Flying Farmers association 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Visit to the JIC by a farming group. Presentation given in an informal manner provocking discussion session. Furhte rdiscussions held over an informal lunch.
Year(s) Of Engagement Activity 2018
 
Description Workshop JIC-CAAS on oilseed rape 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Workshop with Chinese colleagues from Wuhan university to explore potential for collaboration and joint grant applications.
Year(s) Of Engagement Activity 2017
 
Description Workshop JIC-CAAS on oilseed rape 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Workshop and discussions with staff from OCRI-CAAS on future collaborations
Year(s) Of Engagement Activity 2017