a state of the art facility for the study of protein trafficking in vivo
Lead Research Organisation:
University of Leeds
Department Name: Institute of Membrane & Systems Biology
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Determining protein localisation and dynamics is important for answering many questions in biology. To understand how proteins function and are regulated in vivo, we need approaches by which we can determine where proteins go and when, as well as when and where two proteins interact. New and emerging technologies will go a long way towards helping us answer these questions. The first is the development of photo-activatable GFP (PA-GFP). By tagging proteins with PA-GFP, and then using photo-activation to observe a subset of fluorescently labelled molecules on a low fluorescent background, their fate can be accurately determined. The second is the recent developments in the GFP and RFP fluorophores that have improved behaviour in FRET, enabling us to use this approach to investigate protein-protein interactions in vivo. Furthermore, microscopy is now being developed as a tool for high throughput screening approaches, to investigate the effects of mutations, or for screening large numbers of small molecules for ones that have useful effects in cell biology. The major goal of this application is to upgrade our existing bio-imaging facility into a state-of-the-art facility that can exploit these new technologies, with the focus of studying protein trafficking in vivo.
Publications
Mankouri J
(2008)
A comparative cell biological analysis reveals only limited functional homology between the NS5A proteins of hepatitis C virus and GB virus B.
in The Journal of general virology
Hess K
(2012)
A novel mechanism for hypofibrinolysis in diabetes: the role of complement C3.
in Diabetologia
Foresti O
(2010)
A recycling-defective vacuolar sorting receptor reveals an intermediate compartment situated between prevacuoles and vacuoles in tobacco.
in The Plant cell
Brown L
(2011)
A small molecule with differential effects on the PTS1 and PTS2 peroxisome matrix import pathways
in The Plant Journal
Jackson BR
(2011)
An interaction between KSHV ORF57 and UIF provides mRNA-adaptor redundancy in herpesvirus intronless mRNA export.
in PLoS pathogens
Wilshaw SP
(2008)
Biocompatibility and potential of acellular human amniotic membrane to support the attachment and proliferation of allogeneic cells.
in Tissue engineering. Part A
Dove B
(2006)
Cell cycle perturbations induced by infection with the coronavirus infectious bronchitis virus and their effect on virus replication.
in Journal of virology
Groppelli E
(2010)
Cell entry of the aphthovirus equine rhinitis A virus is dependent on endosome acidification.
in Journal of virology
Turrell SJ
(2012)
Cellular uptake of highly-functionalized ruthenium(II) tris-bipyridine protein-surface mimetics.
in Bioorganic & medicinal chemistry letters
Dove B
(2006)
Changes in nucleolar morphology and proteins during infection with the coronavirus infectious bronchitis virus
in Cellular Microbiology
Harrison SM
(2007)
Characterisation of cyclin D1 down-regulation in coronavirus infected cells.
in FEBS letters
Reed ML
(2007)
Characterization of the nuclear export signal in the coronavirus infectious bronchitis virus nucleocapsid protein.
in Journal of virology
Scott DJ
(2011)
Clot architecture is altered in abdominal aortic aneurysms and correlates with aneurysm size.
in Arteriosclerosis, thrombosis, and vascular biology
Henderson Z
(2010)
Co-localization of PRiMA with acetylcholinesterase in cholinergic neurons of rat brain: an immunocytochemical study.
in Brain research
Manna P
(2010)
Constitutive Endocytic Recycling and Protein Kinase C-mediated Lysosomal Degradation Control KATP Channel Surface Density
in Journal of Biological Chemistry
Dugan GE
(2009)
Dependence of the localization and function of the human cytomegalovirus protein US6 on the transporter associated with antigen processing.
in The Journal of general virology
Ghosh SR
(2010)
Determination of the mobility of novel and established Caenorhabditis elegans sarcomeric proteins in vivo.
in European journal of cell biology
Wu W
(2012)
Different NF-?B activation characteristics of human respiratory syncytial virus subgroups A and B.
in Microbial pathogenesis
Dunn S
(2008)
Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells
in Journal of Cell Science
Smith A
(2010)
Direct endosomal acidification by the outwardly rectifying CLC-5 Cl - /H + exchanger
in The Journal of Physiology
Henderson Z
(2010)
Distribution and role of Kv3.1b in neurons in the medial septum diagonal band complex.
in Neuroscience
Peckham M
(2008)
Engineering a multi-nucleated myotube, the role of the actin cytoskeleton.
in Journal of microscopy
Description | The aim of this project was to improve our ability to use light microscopy to image cells, and within cells. The funding allowed us to buy additional equipment to upgrade our existing confocal microscopes, so that we could improve our imaging. These microscopes are used by over 20 different research groups within the Faculty of Biological Sciences at the University, and have supported a wide range of research, from imaging organelles and how they move in living plants, to imaging receptors in mammalian cells. |
Exploitation Route | The research can be used by those interested in developing treatments for infections and disease (e.g. pharma companies, clinicians). Imaging is central to understanding the healthy human organism, plants and animals. Without knowledge of how things work, it is very difficult to understand what goes wrong in disease states. The new microscopes are essential for using imaging to understand cellular processes, and in detecting what goes wrong in diseases from virus infections, to inherited mutant |
Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
URL | http://www.fbs.leeds.ac.uk/facilities/bioimaging/ |
Description | This funding provided an upgrade to our bio-imaging facility which is used by over 40 different research groups across biological sciences and medicine. It has had impact in a broad range of healthcare and biological sciences. |
First Year Of Impact | 2007 |
Sector | Healthcare,Other |
Impact Types | Economic |