New technology to study T lymphocyte ageing in vitro and in vivi
Lead Research Organisation:
University College London
Department Name: Immunology and Molecular Pathology
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
People |
ORCID iD |
Arne Akbar (Principal Investigator) |
Publications
Agius E
(2009)
Decreased TNF-alpha synthesis by macrophages restricts cutaneous immunosurveillance by memory CD4+ T cells during aging.
in The Journal of experimental medicine
Akbar A
(2016)
Senescence of T Lymphocytes: Implications for Enhancing Human Immunity
in Trends in Immunology
Akbar AN
(2017)
The convergence of senescence and nutrient sensing during lymphocyte ageing.
in Clinical and experimental immunology
Covre LP
(2018)
Circulating Senescent T Cells Are Linked to Systemic Inflammation and Lesion Size During Human Cutaneous Leishmaniasis.
in Frontiers in immunology
Rivino L
(2015)
Virus-specific T lymphocytes home to the skin during natural dengue infection.
in Science translational medicine
Seidel JA
(2018)
Skin resident memory CD8+ T cells are phenotypically and functionally distinct from circulating populations and lack immediate cytotoxic function.
in Clinical and experimental immunology
Shih BB
(2017)
Derivation of marker gene signatures from human skin and their use in the interpretation of the transcriptional changes associated with dermatological disorders.
in The Journal of pathology
Vukmanovic-Stejic M
(2013)
Varicella zoster-specific CD4+Foxp3+ T cells accumulate after cutaneous antigen challenge in humans.
in Journal of immunology (Baltimore, Md. : 1950)
Vukmanovic-Stejic M
(2018)
Enhancement of cutaneous immunity during aging by blocking p38 mitogen-activated protein (MAP) kinase-induced inflammation.
in The Journal of allergy and clinical immunology
Vukmanovic-Stejic M
(2015)
The Characterization of Varicella Zoster Virus-Specific T Cells in Skin and Blood during Aging.
in The Journal of investigative dermatology
Title | Akbar Film |
Description | Video of Research Activities in The Akbar Group |
Type Of Art | Film/Video/Animation |
Year Produced | 2015 |
Impact | The film is used by the Akbar laboratory and its members when advertising our research and its impact. It has been shown at public engagement events and used when advertising new opportunities to join the group. |
URL | https://youtu.be/l_426Pamxcc |
Description | The main aims of this grant were twofold. The first aim was to determine whether immune senescence could be regulated at the level of nuclear transport of key molecules from the cytoplasm to the nucleus of human T cells. The second aim of this grant was to investigate why immune responses in the skin are decreased in older individuals. In order to determine the nature of this defect, we investigated leucocyte recruitment and function in the skin Aim 1. We found that T cell senescence was unlikely to be regulated at the nucleopore since T cells at different stages of differentiation showed the same number and distribution of these complexes. Furthermore, there were no differences in expression of neucleopores after activation of these cells. Our previous data had shown that senescent T cells had deficient transport of key signaling molecules into the nucleus of the cells and the data that was generated with the Coutavas Lab suggests that this is probably due to failure of the transport mechanisms but not the entry ports into the nucleus. Aim2. In order to determine the nature of the defect in older individuals, we investigated leucocyte recruitment and function in the skin. Some of this data is still being analysed but the data generated thus far suggests that an essential early signal that amplify the skin response to antigen is considerably reduced in old humans. This responses revolves around the cytokine IFN-gamma, which was an unexpected finding and this work has led to a new testable hypothesis that skin resident T cells, that secrete this cytokine, are defective in old subjects. |
Exploitation Route | For aim 2- we have generated banks of tissue samples for microarray anlaysis which can be used to probe molecular changes during ageing and this is a resaurce that will be available to wider scientific community. |
Sectors | Pharmaceuticals and Medical Biotechnology |
Description | Sanofi Pasteur collaboration |
Amount | £15,000 (GBP) |
Funding ID | 11-0584-zostavax |
Organisation | Sanofi Pasteur MSD |
Sector | Private |
Country | United Kingdom |
Start | 02/2013 |
End | 02/2014 |
Description | Sanofi Pasteur collaboration |
Amount | £15,000 (GBP) |
Funding ID | 11-0584-zostavax |
Organisation | Sanofi Pasteur MSD |
Sector | Private |
Country | United Kingdom |
Start | 02/2013 |
End | 02/2014 |
Description | Collaboration with the Rockefeller University, to generate and analyse microarray data of normal and antigen challenged human skin from young and old individuals to investigate the mechanisms for age related changes in cutaneous immunity. |
Organisation | Rockefeller University |
Country | United States |
Sector | Academic/University |
PI Contribution | This collaboration provided the expertise and the facilities to generate a unique resource: transcriptional data sets of normal and antigen challenged human skin from young and old individuals to investigate the mechanisms for age related changes in cutaneous immunity. This is a unique resource which allows us (and others) to examine age related changes at a transcriptional level in normal skin and skin challenged with recall antigen. |
Collaborator Contribution | This collaboration provided the expertise and the facilities to generate a unique resource: transcriptional data sets of normal and antigen challenged human skin from young and old individuals to investigate the mechanisms for age related changes in cutaneous immunity. This is a unique resource which allows us (and others) to examine age related changes at a transcriptional level in normal skin and skin challenged with recall antigen. |
Impact | doi: 10.1038/jid.2015.63. |
Start Year | 2012 |