Application of the SMALP system to generate antibodies for intact transmembrane proteins
Lead Research Organisation:
University of Birmingham
Department Name: Cancer Sciences
Abstract
The analysis and exploitation of transmembrane proteins has recently become one of the most exciting areas of drug discovery and biological research. About a third of all proteins are found in membranes, their mechanisms are particularly difficult to study as they are often present only in small amounts and are difficult to handle. These proteins are stabilized by their interactions with native lipids but current methods to study them depend on detergents which strip these lipids away. We have developed a novel bionanoparticle to prepare functionally intact proteins directly from native membrane maintaining the local lipid environment and propose to develop antibodies to these targets for therapeutic and research purposes. This new generation of antibodies will allow specific recognition of native protein states with unprecedented accuracy, and will enable development of new diagnostic tests and biotherapeutic agents for treatment of cancer and many infectious and genetic diseases.
Organisations
Publications
Lee SC
(2016)
A method for detergent-free isolation of membrane proteins in their local lipid environment.
in Nature protocols
Gulati S
(2014)
Detergent-free purification of ABC (ATP-binding-cassette) transporters
in Biochemical Journal
Lee SC
(2016)
Encapsulated membrane proteins: A simplified system for molecular simulation.
in Biochimica et biophysica acta
Jamshad M
(2015)
G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent.
in Bioscience reports
Esmaili M
(2018)
Membrane biology visualized in nanometer-sized discs formed by styrene maleic acid polymers.
in Biochimica et biophysica acta. Biomembranes
Overduin Michael
(2014)
MODA, HADDOCK, and SMALP: new developments for unravelling membrane protein structure and function: Honorary Lecture
in BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
Rajesh S
(2016)
NMR of Membrane Proteins: Beyond Crystals.
in Advances in experimental medicine and biology
Jamshad M
(2015)
Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins.
in Nano research