The BUG consortium Building Upon the Genome: using H. contortus genomic resources to develop novel interventions to control endemic GI parasites
Lead Research Organisation:
University of Glasgow
Department Name: College of Medical, Veterinary, Life Sci
Abstract
Most food-producing animals are infected with a variety of different roundworms, or nematodes, many of which live in the digestive tract. These parasites cause a range of disease in their hosts, from diarrhoea and anaemia, to death, in severe cases. They are a major welfare issue and cause significant economic losses to farmers, in terms of reduced production and treatment. It is estimated that these worms cost the UK sheep industry a conservative £84 million per annum. At the present time these parasites are controlled using drugs known as anthelmintics, but many of these chemicals no longer work effectively because the parasites have developed resistance to them, analogous to antibiotic resistance in bacteria. How anthelmintic resistance arises and spreads throughout the worms on a farm is not known. The problem has become so serious in the UK that some sheep-farming enterprises have had to close because the worms can no longer be controlled. Our project will investigate ways of ensuring that the existing drugs are managed to minimise the occurrence and spread of resistance and will also study novel approaches to vaccine development. The current methods for detecting resistant parasites are labour intensive and insensitive, making early detection impossible and analysis of the extent of the problem difficult. This makes it hard to evaluate strategies aimed at reducing the spread of resistance. We need to develop more sensitive methods to detect resistant parasites and so must understand the genetic changes in the parasites that lead to resistance. We have recently sequenced the genome of one of these parasites called Haemonchus contortus (the Barber's pole worm) and found it to be large and complex, containing a similar number of genes to the human genome. Studying this genome will identify novel ways of controlling nematode infections and our research is aimed at using new technologies to ensure that farmers can continue to produce livestock effectively. We plan to use information from the genome to identify markers of drug resistance in Haemonchus and in a closely related worm called Teladorsagia circumcincta, which is the most prevalent parasite found in the UK sheep population. By identifying the genetic changes in worms exposed to anthelmintics, we aim to understand how resistance arises and to develop markers to identify resistant worms on sheep farms. This information will be used to help model how resistance spreads and to investigate how different treatment schedules affect the development of resistance, taking into account climate change scenarios. In the longer term, we would like to develop vaccines against these worms. Although many attempts have been made to do this, most have not been successful, as we have no way of assessing whether the antigens tested to date are the most effective at stimulating a host immune response. However, our project will provide the basis for identifying genes that are under selection by the sheep immune system and therefore likely to be antigenic. We will combine results from this study with knowledge of where and when specific antigens are expressed in the worm to optimise the selection of potential vaccine candidates. Finally, the results from this project will be used to design sustainable control strategies for sheep parasites in the face of anthelmintic resistance and climate change. We have robust plans to engage with the farming community through workshops and publications to ensure that the implications of our work are translated into good farming practice. While this project focuses on parasites of sheep, we anticipate that much of what we learn about anthelmintic resistance will be directly applicable to parasites of cows, horses and pigs where anthelmintic resistance is an emerging problem. Moreover, as the same drugs are increasingly used to control related worms in humans, our work will also impact on human health.
Technical Summary
The aim of this project is to provide sustainable control of nematode parasites of sheep based on an improved understanding of AR and the identification of novel vaccine candidates. Nematodes from two sources will be sequenced: H. contortus from a genetic backcross between IVM-sensitive and resistant lines and T. circumcincta and H. contortus from farms with current clinical AR problems. We will use a method of genotyping by sequencing (ddRAD-Seq) that can be applied to large numbers of individuals, and whole genome sequencing of pools of larvae. This population genetics approach will be underpinned by significant improvements in the genomes of both nematodes based upon the construction of a genetic map for H. contortus and high coverage, individual worm sequencing for T. circumcincta. Markers of AR will be validated in a UK-wide bio-bank containing both parasite species, collected from farms with known histories of anthelmintic usage over several years. Analysis of AR-conferring regions of the genome will reveal the underlying mechanisms, which will be tested in a C. elegans transgenic model system. Once identified, AR markers will be used to study the origin and spread of resistance alleles and to model and assess the effects of different anthelmintic regimes on the development of AR. Additional modelling studies will address the impact of climate change on worm survival and effective refugia and will seek to future-proof any new management strategies. Sequencing data will be employed in a population genomics approach to vaccine discovery with the aim of identifying novel candidate antigens for future development. The study will be underpinned by a cost-benefit analysis of new management strategies and a knowledge exchange module that will involve close liaison with the farming industry to identify the optimal means of implementing new strategies.
Planned Impact
The beneficiaries of this project include the farming community and their advisors, the pharmaceutical industry and the general public. Our project will identify markers of AR and will improve our understanding of the mechanisms and spread of resistance throughout populations. Although much of the knowledge produced will be fundamental, it has the potential to have significant impact in the UK and internationally where the problems of AR are even more acute. The sLoLa will support innovation in science by applying new technologies and generating novel tools for parasitic nematode research. The project will provide excellent training opportunities for the post-docs in genetic, genomic and quantitative approaches to complex biological problems. We will ensure interactions amongst the post-docs by hosting training periods in each Institute. The group at WTSI contribute to several courses and workshops and so have significant experience of training in genomics and next-generation sequence data analysis. Skills acquired during the course of the project will be applicable to many other organisms and will equip the PDRAs for future careers in academia, the bio-tech or pharmaceutical industries.
We will generate data that are of considerable interest to the pharmaceutical industry, some of whose drugs are now largely ineffectual in various parts of the world. Our work will generate tools to detect and monitor resistance, which will be used to improve strategies to mitigate the development of AR. By shedding new light on the mechanisms of resistance, the results will influence the design and use of future anthelmintics and may identify opportunities to restore efficacy to current drugs. Although much effort has been invested in developing a vaccine for H. contortus, most attempts to protect sheep using these antigens in recombinant format have failed. We currently have no way of determining whether the antigens tested to date are the most effective, as they were not selected based on their association with protection in the field. A population genomics approach allows the identification of antigens under selection by the host immune system. It will also identify genes whose products are essential for survival, information that could be exploited in other ways, such as the design of novel inhibitors.
This project has the potential to impact upon farming practices in the UK and elsewhere. Previous advice such as 'dose and move', appears to result in an increased selection for resistance, but is still widely used. The SCOPS (Sustainable Control Of Parasites of Sheep) initiative provides farmers and vets with valuable up-to-date information and guidelines for parasite control but we still lack a basic understanding of the mechanisms of AR on which to base this advice. The development of effective parasite control strategies would improve animal health and welfare, the quality and safety of British lamb and the economic viability of the UK sheep industry. We will engage with farmers and vets through publications in the farming press, attendance at agricultural meetings, veterinary CPD courses, and workshops to determine how findings from this project can be used to establish the best means to improve farm profitability and animal welfare in both the short and the long term. Worm control in dairy herds was recently estimated to cost the UK economy £281 million per annum and the impact of AR would significantly raise that figure. Thus our work has the potential to contribute to the economy by increasing the efficiency of livestock production and safeguarding the industry against an inevitable future challenge. By helping produce high quality meat with excellent standards of animal welfare we hope to influence the public perception of British farming. We have opportunities to interact with the public at events such as the Royal Highland Show and with school children through engagement activities, such as science clubs.
We will generate data that are of considerable interest to the pharmaceutical industry, some of whose drugs are now largely ineffectual in various parts of the world. Our work will generate tools to detect and monitor resistance, which will be used to improve strategies to mitigate the development of AR. By shedding new light on the mechanisms of resistance, the results will influence the design and use of future anthelmintics and may identify opportunities to restore efficacy to current drugs. Although much effort has been invested in developing a vaccine for H. contortus, most attempts to protect sheep using these antigens in recombinant format have failed. We currently have no way of determining whether the antigens tested to date are the most effective, as they were not selected based on their association with protection in the field. A population genomics approach allows the identification of antigens under selection by the host immune system. It will also identify genes whose products are essential for survival, information that could be exploited in other ways, such as the design of novel inhibitors.
This project has the potential to impact upon farming practices in the UK and elsewhere. Previous advice such as 'dose and move', appears to result in an increased selection for resistance, but is still widely used. The SCOPS (Sustainable Control Of Parasites of Sheep) initiative provides farmers and vets with valuable up-to-date information and guidelines for parasite control but we still lack a basic understanding of the mechanisms of AR on which to base this advice. The development of effective parasite control strategies would improve animal health and welfare, the quality and safety of British lamb and the economic viability of the UK sheep industry. We will engage with farmers and vets through publications in the farming press, attendance at agricultural meetings, veterinary CPD courses, and workshops to determine how findings from this project can be used to establish the best means to improve farm profitability and animal welfare in both the short and the long term. Worm control in dairy herds was recently estimated to cost the UK economy £281 million per annum and the impact of AR would significantly raise that figure. Thus our work has the potential to contribute to the economy by increasing the efficiency of livestock production and safeguarding the industry against an inevitable future challenge. By helping produce high quality meat with excellent standards of animal welfare we hope to influence the public perception of British farming. We have opportunities to interact with the public at events such as the Royal Highland Show and with school children through engagement activities, such as science clubs.
Publications
Doyle SR
(2020)
Genomic and transcriptomic variation defines the chromosome-scale assembly of Haemonchus contortus, a model gastrointestinal worm.
in Communications biology
Doyle SR
(2019)
Genome-wide Approaches to Investigate Anthelmintic Resistance.
in Trends in parasitology
Doyle SR
(2019)
Evaluation of DNA Extraction Methods on Individual Helminth Egg and Larval Stages for Whole-Genome Sequencing.
in Frontiers in genetics
Doyle SR
(2022)
Genomic landscape of drug response reveals mediators of anthelmintic resistance.
in Cell reports
Durrant C
(2020)
Population genomic evidence that human and animal infections in Africa come from the same populations of Dracunculus medinensis.
in PLoS neglected tropical diseases
Description | This was a multi-centre award with different partners undertaking different aspects of the project. During the course of the 5-year project there were a few staff changes/movements. Prof Alistair Stott (SRUC) retired and his role as PI was assumed by Dr Catherine Milne, who subsequently retired before the project ended. Both the PI and post-doc from the University of Bristol re-located: Professor Morgan to Queen's University, Belfast and Dr Hannah Vineer to a Fellowship at the University of Liverpool. The remaining two PDRA and the Research Co-investigator all obtained Fellowship positions: Dr Roz Laing, a Wellcome Trust Clinical Fellowship in Veterinary Medicine, Dr Stephen Doyle, a UKRI Future Leaders Fellowship and Dr Umer Chaudhry, a Fellowship from the University of Surrey. Dr Chaudhry has since moved to St Georges University, Grenada, West Indies. At the Wellcome Sanger Institute (WSI) significant progress was made in refining the Haemonchus contortus genome, with the assembly now representing entire chromosomes. This was a major achievement since the publication of the draft genome in 2013 and was made possible by the application of long-read sequencing technologies (PacBio), optical mapping and intensive manual curation. The assembly and annotation are publicly available on WormBase Parasite (https://parasite.wormbase.org/Haemonchus_contortus_prjeb506/Info/Index/), providing a significant genomic resource for a broad group of Clade V nematodes, which include parasitic species of major veterinary and medical importance. This resource removes some of the dependency on Caenorhabditis elegans as a reference for which parasite-specific traits are not relevant. The chromosome-scale assembly now allows insight into chromosome evolution amongst these species and offers a robust scaffold for genome-wide analyses of important parasite traits such as anthelmintic resistance. A paper describing the assembly and annotation was published in 2020 (Doyle et al, 2020, Commun Biol. 2020, 3:656). Work is still underway to sequence and assemble the genome of the closely related species, Teladorsagia circumcincta, which is the major gastrointestinal parasite of sheep in the UK. Assembling the genome of this species has been particularly challenging, as it has a genome size approximately double that of H. contortus that is complex and highly polymorphic. Teladorsagia circumcincta DNA prepared at the University of Glasgow has been used for short and long read sequencing, as well as for optical mapping, which collectively have been used to assemble a draft T. circumcincta genome at the WSI. The assembly has been further improved since the completion of the BUG project; taking advantage of recent advances in in situ Hi-C (chromatin conformation capture) short read sequencing which allowed scaffolding of the majority of the assembly into six large scaffolds, syntenic to the H. contortus chromosomes, with additional shorter scaffolds remaining. The assembly is now in the final stages of curation before it is "frozen" and subsequently annotated; however, preliminary analyses against closely related genomes have shown that this new assembly represents a significant and meaningful improvement for population genomic analysis over existing resources for T. circumcincta. For both parasites, additional RNA sequencing was carried out on samples prepared from defined life cycle stages using the long-read Iso-Seq method to enable annotation. At the University of Edinburgh and the Moredun Research Institute (MRI), efforts initially concentrated on establishing a new genetic cross between an anthelmintic sensitive (MHco3) and a multi-drug resistant isolate (MHco18) of H. contortus and the preliminary characterisation of the F1 progeny. In Glasgow, DNA was isolated from a single MHco3 female and her F1 progeny for genotyping at WSI and the construction of a genetic map. The genetic map is complete and sib-ship analysis confirmed the polyandrous nature of female H. contortus, with progeny sequenced from the single female worm originating from at least eight male worms. In addition, triploid progeny were also identified. A paper summarising this work was published (Doyle et al., 2018, Genome Biology and Evolution 10:396-409). The F2 population were used to infect additional animals, which were then treated with one of three drugs. Genotyping of pools of L3 recovered pre- and post-treatment has allowed a bulk segregant analysis to be carried out. Different regions of the genome were shown to be under selection by each of the three drugs, confirming distinct modes of action. For benzimidazole and levamisole, one major and several minor regions under selection were identified. For ivermectin, a more complex signal of selection was observed, which may be indicative of several genes contributing to resistance. A large region on Chromosome V was identified and efforts focused on narrowing down the region and confirming the genes involved. Significantly, a similar locus on Chromosome V was also identified in a recent analysis of two geographically distinct ivermectin-resistant lines, based on a pre-existing genetic cross but which has been completed for publication as part of the consortium's work (Doyle et al. 2019, BMC Genomics, 20, 218). Work progressed to narrow down this region and resulted in the identification of a major QTL associated with ivermectin resistance in the F2 population of the genetic cross. A variety of other specimens were prepared for sequencing including individual H. contortus larvae from an in vitro resistance assay (DrenchRite development assay), that allowed susceptible larvae and L3 that were highly resistant to ivermectin to be separated. Sequencing of these worms identified the same QTL. Finally, L3 from a number of USA farms with differing levels of anthelmintic resistance were sequenced and the same QTL identified on Chromosome V. Of the 25 genes contained within this region, cky-1 was chosen for further study. cky-1 was selected on the basis that it is involved in regulating neuronal/behavioural plasticity, was highly upregulated in a transcriptomic study of male and female worms from both parental isolates and the F2 generation of the cross, with and without IVM exposure (see below). It was also upregulated in additional geographically distinct ivermectin resistant strains of H. contortus. The effects of mutation or over-expression of cky-1 were studied in C. elegans using a range of assays (development and pharyngeal pumping) to determine whether they do indeed play a role in IVM resistance. C. elegans worms carrying a deletion in cky-1 or worms in which cky-1 levels were reduced by RNAi both showed increased sensitivity to ivermectin, further implicating cky-1 in ivermectin resistance. This work is published in Doyle et al, 2022, Cell Reports, 41, 111522. As referred to above, transcriptomic analysis was also carried on parental worms and the cross in order to help prioritise candidate genes in the genomic locus under IVM selection. As expected there were many constitutive differences in gene expression in the parental samples (MHco3 versus MHco18). However, genes that were also differentially expressed in pairwise comparisons of the F2 generation of the cross with and without IVM exposure represent a high confidence set of genes associated with IVM resistance. This analysis identified a number of genes of interest and highlighted the likely role of cky-1 in ivermectin resistance and is published in Laing et al 2022, PLoS Pathogens, 18 (6)e:1010545. In Edinburgh, Bristol and MRI, field samples of T. circumcincta were collected pre- and post-drug treatment from sheep farms in different regions of the UK (Evans et al 2021, Int J Parasitol. 2021, 51 (5):393-403). At Glasgow, the larvae were processed for genotyping using ddRAD-Seq and whole genome pooled sequence at WSI. The data arising from this part of the project were previously analysed, but the fragmented nature of the assembly of the T. circumcincta genome at that time limited the analysis. The new and improved genome assembly has allowed the re-analysis of the Pool-Seq data, and an intermediate draft of this assembly has shown a significant improvement, with clearly defined loci differentiated following ivermectin treatment of field populations. The data will be re-analysed once more following the final curation of the genome assembly. At the University of Edinburgh, the focus was on studying the emergence and spread of benzimidazole resistance. A novel deep amplicon sequencing method based on the Illumina Mi-seq platform was used to study the phylogenetics of benzimidazole resistance mutations in field samples of T. circumcincta collected from three UK sheep flocks, selected to represent different aspects of sheep management and approaches to parasite control. Different frequencies of benzimidazole resistance mutations were seen in T. circumcincta in the three UK sheep flocks. Benzimidazole resistance mutations had nearly reached genetic fixation in the three-year study flock, highlighting a need for more refined knowledge of the dynamics of single or multiple resistance haplotypes and how their frequency changes over time. Such information will allow testing of hypotheses with reference to the selection pressures that lead to differences in resistance allele frequencies between sampling dates and animal groups. Work at MRI has also focused on benzimidazole resistance; pyrosequencing of ß-tubulin isotype 1 SNPs associated with BZ resistance was undertaken on individual larvae of the H. contortus isolates and different generations of the crosses: MHco3 females crossed with MHco18 males and MHco18 females crossed with MHco3 males to gain an improved understanding of genetic selection of BZ resistance using genotypic and phenotypic readouts (i.e. does mating between resistant male worms and susceptible female worms result in greater or lesser phenotypic resistance?). The results showed that a heterozygote resistance genotype at both P167 and 200 of ß-tubulin isotype 1 appeared to result in greater phenotypic expression of resistance in F1 generations compared to homozygote resistance at either position. In a partnership between Glasgow and MRI, additional funding was obtained from the Natural Environment Research Council (NERC) for a CASE PhD project to follow up on the phenotypic consequences and genetic basis of resistance to ivermectin. Here work focused on whether drug-susceptible and resistant worms were competent to mate productively and the metabolic consequences of exposure to ivermectin. In addition, a second studentship was granted from the University of Glasgow to work on levamisole resistance. Both students started in October 2018. Work under the levamisole project built upon the findings of the QTL analysis and focused on a gene, acr-8, previously associated with levamisole resistance. A single novel non-synonymous variant in acr-8 was identified and was strongly correlated with levamisole resistance in the QTL analysis. To discriminate between the "susceptible" and "resistant" genetic variants, an allele-specific PCR was developed (Antonopoulus et al, 2022, International Journal of Parasitology, Drugs and Drug Resistance, 20, 17-26) and subsequently, a LAMP assay was also developed. Collaboration with the group of Dr Cedric Nevue in Tours, France using their Xenopus oocyte expression system, showed that expression of the resistance-associated variant conferred a reduced sensitivity to levamisole. At the University of Glasgow, work focused on the use of computer models to study the spread of anthelmintic resistance in sheep nematodes. By modelling the frequency of resistance alleles and their associated genotypes within the worm population as they change in response to treatment, it was possible to highlight patterns in the emergence of resistance. These patterns depended both on how resistance is encoded (one gene versus many), and on the treatment regimens implemented. Data collected by other members of the consortium can be fitted to the models and predict the treatment regimens (such as treatment frequency and use of refugia) that might prolong the effectiveness of anthelmintic drugs. In work initiated at the University of Bristol (and being continued at QUB and University of Liverpool), models for the weather-dependent population dynamics of T. circumcincta and H. contortus were validated and an extended model incorporating the development of anthelmintic resistant phenotypes, grazing (and refugia) management, and anthelmintic treatments was completed (Rose Vineer et al 2019, International Journal for Parasitology, 49, 901-910). The model's underlying simplified representation of the ivermectin-resistant genotype was consistent with the output from WSI and the model complements the more complex representation of the genotype in the Glasgow model. Simulations representing combinations of anthelmintic treatment and grazing patterns suggest that weather and climate play an important role in the speed of development of anthelmintic resistance; the predicted increase in resistance allele frequency following treatment varied year to year and between climatic zones. The objectives of the project were disseminated to stakeholders, alongside a request for reports of poor drug efficacy on farms in the south-west in order to source relevant H. contortus field isolates. This was achieved through local meetings of sheep farmers (including the Sheep SW event) and engagement with local veterinary practices, APHA and AHDB. Potential format and utilisation of decision support tools planned within the project were discussed with representatives of SCOPS, AHDB, NFU and APHA. This engagement continued to be actively developed as the predictive modelling platform was built and adapted to decision support by both Prof Morgan and Dr Vineer, in their new positions. At the SRUC, four in-depth workshops to obtain information about worm control practices used by farmers and the obstacles to implementing current good practice were completed. The results highlighted the need for clearly defined recommendations for more sustainable control and that complexity is one of the main challenges in communication of key messages. A prototype stochastic simulation model to estimate the financial effects of AR and its management was also constructed and further developed using outputs from models developed by Prof Morgan, Dr Vineer and colleagues. Potential links with the modelling work being undertaken at the University of Glasgow were explored. This work has also stimulated significant discussion on our online project management site and clearly indicates that the benefits of integrating social science with 'hard' science.' In all, six BUG Consortium progress meetings were held (three in Glasgow, one each in Liverpool, Edinburgh and Cambridge) and attended by all project partners and the Advisory Panel. Regular updates were posted on the BUG Consortium website, blog and twitter accounts. |
Exploitation Route | The availability of the H. contortus genome assembled to chromosomal level is a very significant advance in the field and of great value to other scientists working on parasitic nematodes. Identification of the loci under selection by each of three drugs in use is of great interest to the livestock research community, to those working on resistance in human helminths and potentially to pharmaceutical companies. Using the QTL data, we were able to identify a single gene affecting ivermectin resistance in H. contortus, to refine the role of acr-8 in levamisole resistance and to develop a LAMP assay which would be of value for testing for levamisole resistance in the field. Advances such as these will impact the development of various production methods aimed at slowing the spread of resistance. The data coming from modelling aspects of the project is also of relevance for understanding how best to achieve strategic control of parasitic nematodes. Finally, the workshops and outreach by many members of the BUG consortium have allowed good contact and feedback from stakeholders and the relevant agricultural communities. |
Sectors | Agriculture Food and Drink |
URL | https://bugconsortium.wordpress.com |
Description | An integrated approach to tackling drug resistance in livestock trypanosomes. |
Amount | £343,273 (GBP) |
Funding ID | BB/S000143/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2019 |
End | 12/2020 |
Description | BBSRC KTN CASE Award |
Amount | £150,000 (GBP) |
Funding ID | BB/N50385X/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2015 |
End | 09/2019 |
Description | BSAS Steve Bishop Early Career Award |
Amount | £5,000 (GBP) |
Organisation | Knowledge Transfer Network |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 08/2020 |
End | 09/2021 |
Description | NERC CASE Award |
Amount | £105,000 (GBP) |
Funding ID | NE/R008183/1 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 09/2018 |
End | 09/2022 |
Description | Supporting the National Action Plan for Antimicrobial Resistance (SNAP-AMR) in Tanzania |
Amount | £3,189,370 (GBP) |
Funding ID | MR/S004815/1 |
Organisation | Medical Research Council (MRC) |
Sector | Public |
Country | United Kingdom |
Start | 04/2018 |
End | 04/2023 |
Description | The generation and maintenance of genetic novelty in helminth populations |
Amount | £1,041,940 (GBP) |
Funding ID | MR/T020733/1 |
Organisation | Medical Research Council (MRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2020 |
End | 08/2025 |
Description | University of Glasgow Scholarship |
Amount | £100,000 (GBP) |
Organisation | University of Glasgow |
Sector | Academic/University |
Country | United Kingdom |
Start | 09/2018 |
End | 03/2022 |
Description | iBAHCM internal funding |
Amount | £3,000 (GBP) |
Organisation | University of Glasgow |
Sector | Academic/University |
Country | United Kingdom |
Start | 03/2017 |
End | 08/2017 |
Title | Additional file 1 of Complete representation of a tapeworm genome reveals chromosomes capped by centromeres, necessitating a dual role in segregation and protection |
Description | Additional file 1: Table S1. Chromosome summary. Table S2. Comparison of one-to-one orthologues between assemblies and other flatworms. Table S3. Gene model annotations and Echinococcus multilocularis orthologues. Table S4. Paralogous expansions within orthologue groups predicted using successive H. microstoma genome assembly versions. Table S5. Assessment of genome completeness based on presence/absence of conserved eukaryotic genes. Table S6. Presence and absence of BUSCO orthologues (v. 3.0.2) missing in = one flatworm. Table S7.1. Differentially expressed gene models in Larvae vs. Whole Adult RNA-seq samples ranked by log2-fold change. Table S7.2. Differentially expressed gene models in Scolex-Neck vs. Mid RNA-seq samples ranked by log2-fold change. Table S7.3. Differentially expressed gene models in Scolex-Neck vs. End RNA-seq samples ranked by log2-fold change. Table S7.4. Differentially expressed gene models in Mid vs. End RNA-seq samples ranked by log2-fold change. Table S7.5. Intersect of gene models up-regulated in the Scolex-Neck cf. Mid and End. Table S7.6. Intersect of gene models up-regulated in the Mid cf. Scolex-Neck and End. Table S7.7. Intersect of gene models up-regulated in the End cf. Mid and Scolex-Neck. Table S8. Repetitive elements summary. Table S9. Repetitive element hotspots. Table S10. Micro-exon genes. Table S11. Trans-spliced genes. Table S11.1. Genomic organisation of spliced leader genes. Table S12. Chromosome fusions between H. microstoma and E. multilocularis. |
Type Of Material | Database/Collection of data |
Year Produced | 2020 |
Provided To Others? | Yes |
URL | https://springernature.figshare.com/articles/dataset/Additional_file_1_of_Complete_representation_of... |
Title | Additional file 1 of Complete representation of a tapeworm genome reveals chromosomes capped by centromeres, necessitating a dual role in segregation and protection |
Description | Additional file 1: Table S1. Chromosome summary. Table S2. Comparison of one-to-one orthologues between assemblies and other flatworms. Table S3. Gene model annotations and Echinococcus multilocularis orthologues. Table S4. Paralogous expansions within orthologue groups predicted using successive H. microstoma genome assembly versions. Table S5. Assessment of genome completeness based on presence/absence of conserved eukaryotic genes. Table S6. Presence and absence of BUSCO orthologues (v. 3.0.2) missing in = one flatworm. Table S7.1. Differentially expressed gene models in Larvae vs. Whole Adult RNA-seq samples ranked by log2-fold change. Table S7.2. Differentially expressed gene models in Scolex-Neck vs. Mid RNA-seq samples ranked by log2-fold change. Table S7.3. Differentially expressed gene models in Scolex-Neck vs. End RNA-seq samples ranked by log2-fold change. Table S7.4. Differentially expressed gene models in Mid vs. End RNA-seq samples ranked by log2-fold change. Table S7.5. Intersect of gene models up-regulated in the Scolex-Neck cf. Mid and End. Table S7.6. Intersect of gene models up-regulated in the Mid cf. Scolex-Neck and End. Table S7.7. Intersect of gene models up-regulated in the End cf. Mid and Scolex-Neck. Table S8. Repetitive elements summary. Table S9. Repetitive element hotspots. Table S10. Micro-exon genes. Table S11. Trans-spliced genes. Table S11.1. Genomic organisation of spliced leader genes. Table S12. Chromosome fusions between H. microstoma and E. multilocularis. |
Type Of Material | Database/Collection of data |
Year Produced | 2020 |
Provided To Others? | Yes |
URL | https://springernature.figshare.com/articles/dataset/Additional_file_1_of_Complete_representation_of... |
Title | Data from: Prediction and attenuation of seasonal spillover of parasites between wild and domestic ungulates in an arid mixed-use system |
Description | 1.Transmission of parasites between host species affects host population dynamics, interspecific competition, and ecosystem structure and function. In areas where wild and domestic herbivores share grazing land, management of parasites in livestock may affect or be affected by sympatric wildlife due to cross-species transmission. 2.We develop a novel method for simulating transmission potential based on both biotic and abiotic factors in a semi-arid system in Botswana. Optimal timing of antiparasitic treatment in livestock is then compared under a variety of alternative host scenarios, including seasonally migrating wild hosts. 3.In this region, rainfall is the primary driver of seasonality of transmission, but wildlife migration leads to spatial differences in the effectiveness of treatment in domestic animals. Additionally, competent migratory wildlife hosts move parasites across the landscape. 4.Simulated transmission potential matches observed patterns of clinical disease in the study area. Increased wildlife contact is correlated with a decrease in disease, suggesting that noncompetent wild hosts may attenuate transmission by removing infective parasite larvae from livestock pasture. 5.Optimising the timing of treatment according to within-year rainfall patterns was considerably more effective than treating at a standard time of year. By targeting treatment in this way, efficient control can be achieved, mitigating parasite spillover from wildlife where it does occur. 6.Synthesis and applications: This model of parasite transmission potential enables evidence-based management of parasite spillover between wild and domestic species in a spatio-temporally dynamic system. It can be applied in other mixed-use systems to mitigate parasite transmission under altered climate scenarios or changes in host ranges. |
Type Of Material | Database/Collection of data |
Year Produced | 2018 |
Provided To Others? | Yes |
URL | https://datadryad.org/stash/dataset/doi:10.5061/dryad.9jj84 |
Title | GLOWORM-PARA: a flexible framework to simulate the population dynamics of the parasitic phase of gastrointestinal nematodes infecting grazing livestock |
Description | Gastrointestinal nematodes are a significant threat to the economic and environmental sustainability of keeping livestock, as adequate control becomes increasingly difficult due to the development of anthelmintic resistance in some systems and climate-driven changes to infection dynamics. To mitigate any negative impacts of climate on gastrointestinal nematode epidemiology and slow anthelmintic resistance development, there is a need to develop effective, targeted control strategies that minimise the unnecessary use of anthelmintic drugs and incorporate alternative strategies such as vaccination and evasive grazing. However, the impacts climate and gastrointestinal nematode epidemiology may have on the optimal control strategy are generally not considered, due to lack of available evidence to drive recommendations. Parasite transmission models can support control strategy evaluation to target field trials, thus reducing the resources and lead-time required to develop evidence-based control recommendations incorporating climate stochasticity. Gastrointestinal nematode population dynamics arising from natural infections have been difficult to replicate and model applications have often focussed on the free-living stages. A flexible framework is presented for the parasitic phase of gastrointestinal nematodes, GLOWORM-PARA, which complements an existing model of the free-living stages, GLOWORM-FL. Longitudinal parasitological data for two species that are of major economic importance in cattle, Ostertagia ostertagi and Cooperia oncophora, were obtained from seven cattle farms in Belgium for model validation. The framework replicated the observed seasonal dynamics of infection in cattle on these farms and overall, there was no evidence of systematic under- or over-prediction of faecal egg counts. However, the model under-predicted the faecal egg counts observed on one farm with very young calves, highlighting potential areas of uncertainty that may need further investigation if the model is to be applied to young livestock. The model could be used to drive further research into alternative parasite control strategies such as vaccine development and novel treatment approaches, and to understand gastrointestinal nematode epidemiology under changing climate and host management. |
Type Of Material | Computer model/algorithm |
Year Produced | 2020 |
Provided To Others? | Yes |
Impact | None yet. The model is currently being used to evaluate the impact of weather on the development of anthelmintic resistance in sheep gastrointestinal nematodes. |
URL | https://livrepository.liverpool.ac.uk/3073365/ |
Title | Genetic markers of anthelmintic resistance in gastrointestinal parasites of ruminants |
Description | |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | Yes |
URL | http://researchdata.gla.ac.uk/id/eprint/975 |
Title | Haemonchus contortus v2.0 genome assembly and annotation |
Description | A greatly improved genome assembly and annotation for Haemonchus contortus, which now represents intact chromosomes for this nematode parasite: the first for one of the major groups of helminth parasites. |
Type Of Material | Database/Collection of data |
Year Produced | 2018 |
Provided To Others? | Yes |
Impact | The assembly has already underpinned research both from our group (publications listed in publication section) and upcoming research by a number of other research groups (e.g. Gilleard and Wasmuth groups, Calgary; Blaxter group, Edinburgh) |
URL | https://parasite.wormbase.org/Haemonchus_contortus_prjeb506/Info/Index |
Title | Increasing importance of anthelmintic resistance in European livestock: creation and meta-analysis of an open database |
Description | A database of European published and unpublished AR research on gastrointestinal nematodes (GIN) and liver fluke (Fasciola hepatica) was collated and combined with data from a previous systematic review of AR in GIN to provide a better understanding of the extent of AR in Europe in order to develop and advocate more sustainable parasite control approaches. The database is analysed in https://doi.org/10.1051/parasite/2020062 and a "live" version has been made openly available at https://doi.org/10.17605/OSF.IO/BQZXV. |
Type Of Material | Database/Collection of data |
Year Produced | 2020 |
Provided To Others? | Yes |
Impact | The database has provided evidence of the increasing importance of anthelmintic resistance in European livestock and identified knowledge gaps and methodological limitations that need to be addressed in future studies/diagnostic tests. In the context of the UK, the database has highlighted areas of increased uncertainty in the available data. |
URL | https://doi.org/10.17605/OSF.IO/BQZXV |
Title | Wormer resistance and moxidectin use in Scottish sheep flocks (WORMSS project) |
Description | Data was gathered during 2021 from 76 sheep farmers across Scotland, focusing on their use and understanding of the ectoparasiticide, moxidectin in their flock. In addition, it contains data from post-moxidectin treatment sheep faecal samples collected from sheep treated by the farmer on six separate farms across Scotland. From each farm two sets of samples were collected, and the data contains information on the faecal egg counts and species identified using PCR of the ITS2 region. The data may be viewed and re-used by others. |
Type Of Material | Database/Collection of data |
Year Produced | 2022 |
Provided To Others? | Yes |
URL | http://researchdata.gla.ac.uk/id/eprint/1240 |
Description | WORMSS (BSAS Steve Bishop Early Career Award) |
Organisation | Flock Health Limited |
Country | United Kingdom |
Sector | Private |
PI Contribution | We have formulated the project concept, obtained ethical permission, and are doing the research. |
Collaborator Contribution | The collaborator provides expert advice, guidance and has assisted in planning the project. They will also assist in dissemination of results. |
Impact | Now understand GDPR/ethics - this is going to be very useful for a future project for which we have obtained funding. Learnt better how to write questionnaires for farmers and reach them - this will be useful for impact of current and future projects. The project is ongoing - research outcomes/impact is in the future. |
Start Year | 2020 |
Description | 26th WAAVP_Chaudhry and Sargison |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited talks: Application of molecular methods and conventional parasitology to understand ovine nematode parasite co-infections, in the absence of intervention; Genomic and genetic approaches to identify loci linked to the anthelmintic resistance in Haemonchus contortus. |
Year(s) Of Engagement Activity | 2017 |
Description | 9th International Sheep Veterinary Congress |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Presentations and engagement by BUG researchers relating to the BUG project. Plenary talk: Improved small ruminant production efficiency through global solutions to local challenges and local solutions to global challenges. Keynote talk: Anthelmintic resistance and sustainable helminth control. Other talks: Comparison of nematode parasite diversity and co-infections between feral and commercially managed sheep; Building upon the Haemonchus genome; Identifying animal health priorities for livestock farmers in rural Malawi; Investigation of productivity in a southern Indian Malabari goat herd shows opportunities for planned animal health management to improve food security; Assessing seasonal changes in strongylid nematode biome structures in a wild sheep population; A twelve month study of faecal worm egg counts on three farms and the impact of modern management; An evaluation of UK sheep farmers attitudes and behaviours towards sustainable roundworm control; Patterns of faecal nematode egg shedding after treatment of sheep with a long-acting formulation of moxidectin; |
Year(s) Of Engagement Activity | 2017 |
Description | Anthelmintics: from discovery to resistance 111 - Chaudhry |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Talk: Evidence for the genetic linkage of three microsatellite makers to an ivermectin resistance locus in the genetic crossing of Haemonchus contortus strains. |
Year(s) Of Engagement Activity | 2017 |
Description | Anti-microbial resistance scoping meeting Bangalore, India |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | This workshop, held under the auspices of BIS, brought together researchers from India and the UK working on drug resistance in various organisms. |
Year(s) Of Engagement Activity | 2016 |
Description | Berlin Parasitology Seminar series |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited presentation to the Berlin Parasitology Seminar series, which is a forum held between a number of research institutions in Berlin, Germany |
Year(s) Of Engagement Activity | 2021 |
Description | Blog on BUG consortium grant |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | We curate a blog on the BUG project (https://bugconsortium.wordpress.com) |
Year(s) Of Engagement Activity | 2015,2016,2017 |
Description | British Association for Veterinary Parasitolgy talk on modelling sheep GI nematodes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Talk on "Reduced egg shedding in nematode-resistant ewes & epidemiological benefits under climate change" by Hannah Rose Vineer. Attended by 56 members of the British Association of Veterinary Parasitology (including researchers, pharmaceutical industry representatives, students, veterinarians and civil servants). The talk prompted useful feedback on potential model improvements and practical applications. |
Year(s) Of Engagement Activity | 2019 |
URL | http://bavp.org.uk/meeting-news/bavp-winter-meeting-2019-edinburgh/ |
Description | British Cattle Veterinary Association |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | BCVA youngstock course. Parasite control in youngstock at grass: gastrointestinal helminth infections and lungworm. |
Year(s) Of Engagement Activity | 2017 |
Description | CARS keynote presentation |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | invited keynote talk to research scientists, research students and some others at CARS 'consortium on anthelminthic resistance'. (delivered remotely). Focused on BUG research. |
Year(s) Of Engagement Activity | 2021 |
URL | http://www.waavp2021.com/programme/cars-2021/ |
Description | CEED/Anwar seminar at Royal Veterinary College |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Invited presentation on anthelminthic genetics to audience of researchers and students at Royal Veterinary College, London |
Year(s) Of Engagement Activity | 2018 |
Description | COMBAR (Combatting Anthelmintic Resistance in Ruminants) talk |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Hannah Rose Vineer presented on "Modelling the impact of climate and management on AR in GI nematodes" to member of the COMBAR consortium, highlighting the BUG consortium and summarising recent model developments. Generated useful discussion on modelling in general, and potential international extensions to the models. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.combar-ca.eu/sites/default/files/COMBAR%20Meeting%20Report_Ghent.pdf |
Description | CompBio talk 2022 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Other audiences |
Results and Impact | Presentation given to the University of Glasgow Computational Biology group at their annual conference. Included: Teladorsagia genome, recent improvements to it, analysis of Pool-Seq data collected previously, touched on the Haemonchus genome also made by BUG, and the significant improvements having a chromosomal level assembly made to analyses. |
Year(s) Of Engagement Activity | 2022 |
URL | https://cbc.dcs.gla.ac.uk/ |
Description | Conference presentation - Ecology, Evolution and Genomics of C. elegans and other Nematodes. Wellcome Sanger Institute, Cambridgeshire, UK. |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Conference presentation - C. elegans and other nematodes, Hinxton |
Year(s) Of Engagement Activity | 2018 |
Description | Dr Cath Milne ran a workshop on best practice in worm control, Stirling 2018 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Workshop on worm control |
Year(s) Of Engagement Activity | 2018 |
Description | Dr Cath Milne ran a workshop on best practices in worm control in Penrith |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Discussion forum on best practices for control of worms |
Year(s) Of Engagement Activity | 2018 |
Description | Engagement in Pakistan |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Pleanry and invited talks at PARACON: Recent Advances and Emerging Issues in Parasitology. Lahore, Pakistan. 25th-26th October 2017: Global Challenges facing helminth control in small ruminants - lessons for other livestock species; Genomic and genetic approaches to identify loci linked to anthelmintic resistance; The BUG (Building Upon Genome): Genomic and genetic approaches to identify loci linked to the anthelmintic resistance in Haemonchus contortus. Engagement and outreach with undergraduate veterinary and post graduate students and academic and clinical staff at University of Veterinary and Animal Sciences, Lahore: Roundworm control in cattle: why do we need innovative research tools?; The need for improved global small ruminant production: challenges and solutions; Plenary and invited talks at University of Central Punjab: Genetic crossing work in the model parasitic nematode, Haemonchus contortus; Applications of next generation sequencing methods in parasitology research. |
Year(s) Of Engagement Activity | 2017 |
Description | Exlana ram sale |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | A presentation was given by Dr Hannah Rose to sheep farmers and breeders attending the Exlana ram sales. Around 50 farmers attended. The talk focused on utilising predictive modelling to enhance breeding strategy for parasite resistance under climate change, and hence reduce reliance on anthelmintic drugs and slow drug resistance. Material arising from model development under the BUG project was presented. Discussion ensued and farmers reported increased appreciation of the possible changes in epidemiology under climate change, and how breeding strategy should take this into account. |
Year(s) Of Engagement Activity | 2016 |
Description | Farm visits and resistance testing |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Industry/Business |
Results and Impact | As part of sampling within the project, 16 farms in SW England were visited to conduct faecal egg count reduction tests (FECRT), the main method of anthelmintic resistance detection on farms (Dr Hannah Rose, Ms Katie Bull and Dr Eric Morgan participated). Professional teams from the supporting veterinary practice were included in the tests, which supported training of 5 veterinary surgeons and 3 veterinary nurses in correct procedures, including marking, drenching and faecal sampling procedure, and sensitive faecal egg counting methods. The practice team is now capable of conducting FECRT properly and offers this service to clients. |
Year(s) Of Engagement Activity | 2016 |
Description | Final year talk at sponsor AHDB Beef and Lamb's PhD conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | A 'final year' presentation was given about the research to a large group made up of industry workers of a sponsor, AHDB Beef and Lamb, with their dairy and pig partners and others working within the industry. In addition other PhD students of the sponsor and their supervisors were also present, representing many universities and organisations across the UK. Questions and discussions related to the research contined ad hoc over the two day conference, with the chance to also hear about other research in the farming industry and impacts/requirements for further research needed. |
Year(s) Of Engagement Activity | 2018 |
URL | https://ahdb.org.uk/phd-studentships |
Description | Game playing at the Royal Highland Show |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | We made a game "Fishing for worms" to help educate the public about the issue of anthelmintic resistance |
Year(s) Of Engagement Activity | 2016 |
Description | Interdepartmental seminar at the University of Liverpool |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Hannah Rose Vineer presented "Modelling to inform sustainable parasite control" at an internal interdepartmental seminar. Useful feedback on potential future applications of the models. Colleagues also suggested other projects that would benefit from similar approaches. |
Year(s) Of Engagement Activity | 2019 |
Description | Interview with undergraduate students from University of Florida |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Undergraduate students |
Results and Impact | Invited interview with University of Florida undergraduate students to discuss my career and research |
Year(s) Of Engagement Activity | 2022 |
Description | Invited Talk |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | An invited talk was presented by Dr Stephen Doyle at La Trobe University, Melbourne, Australia, entitled "Sex, drugs, and recombination in Haemonchus contortus" |
Year(s) Of Engagement Activity | 2017 |
Description | Invited plenary speaker at 5th Parasitic Nematode Workshop: Bridging the Divide |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited plenary speaker at 5th Parasitic Nematode Workshop: Bridging the Divide |
Year(s) Of Engagement Activity | 2021 |
Description | Invited plenary speaker at Molecular Helminthology: An Integrated Approach |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited plenary speaker to discuss current research, which generated a lot of interesting followup discussion |
Year(s) Of Engagement Activity | 2021 |
Description | Invited poster - Sheep Breeders Round table |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | The Sheep Breeders Roundtable is organised by one of the funding sponsors of the project, AHDB Beef and Lamb, and I was requested to take part. I provided two posters. One on species differences between strongyle populations at different sampling time points/groups of animals and a further poster, building on the first, but looking specifically at the determination of anthelmintic efficacy by faecal egg count reduction tests. I was able to attend the meeting and discuss the content of the posters and the wider project with those who attended. At the same time we were able to make an announcement about the wider BUG project, requesting material. |
Year(s) Of Engagement Activity | 2017 |
URL | https://www.nationalsheep.org.uk/sbrt/ |
Description | Invited presentation at Sheep Vet Society meeting, May 2018 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | I was asked to provide a 20 minute presentation on faecal egg counts, and the range of complicating factors affecting interpretation in practice. I provided an overview of various methods, discussing sensitivities of these and considered strongyle species population composition impact. In addition I looked at some of the more basic steps from collection to culture, which could impact on the number of eggs counted, and the species identified. There were between 50 and 100 vets present, with professionals involved in various other sheep enterprises (eg. breeding), industry etc present also. The talk stimulated discussion, and, at the autumn SVS meeting, further conversations surrounding the topic of faecal egg counting and anthelmintic resistance were had. |
Year(s) Of Engagement Activity | 2018 |
URL | https://www.sheepvetsoc.org.uk/event/spring-meeting-may-2018 |
Description | Invited seminar, Division of infectious diseases, University of Virginia School of Medicine |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited seminar at University of Virginia, audience of professional researchers, students and clinicians. Extensive discussion afterwards about the state of the art of research and knowledge on drug resistance in human helminthiases. |
Year(s) Of Engagement Activity | 2017 |
Description | Invited short talk at Innovative tools for NTD Research Exploiting Genomics at RVC London |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Other audiences |
Results and Impact | Invited talk to discuss ways of integrating genomic approaches into NTD research by the London Centre for Neglected Tropical Disease Research. I spoke about our BUG project genomic analyses, and the parallels that could be used for NTD drug resistance research. My talk was entitled "Building genomic resources for gastrointestinal helminth biology and control". |
Year(s) Of Engagement Activity | 2017 |
Description | Invited tak at International Congress of Parasitology, Deagu, Korea; 19-24th August 2019 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Presented work on anthelminthic genetics to international audience of scientists and public health professionals. |
Year(s) Of Engagement Activity | 2018 |
Description | Invited talk |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A talk was presented at the INRA annual conference Tours, France by Dr Stephen Doyle entitled "Building genomic resources for helminth biology and control" |
Year(s) Of Engagement Activity | 2016 |
Description | Invited talk (keynote) at Anthelmintics III Meeting in Florida |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Invited talk on the use of a genetic cross to study anthelmintic resistance in a parasitic nematode of sheep, attended by roughly 100 participants from around the world, followed by good discussion regarding preliminary findings. |
Year(s) Of Engagement Activity | 2018 |
Description | Invited talk at DIDE Seminar Series, Imperial College London |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Other audiences |
Results and Impact | I was invited to give a Departmental Seminar at the Department of infectious Disease and Epidemiology in Imperial College London. I spoke about my previous work in NTD genomics, and the benefits of using H. contortus genetics to understand anthelmintic resistance. The talk was entitled "Genomic insights into anthelmintic resistance". |
Year(s) Of Engagement Activity | 2017 |
Description | Invited talk at Drug Design and Development conference (German Society for Parasitology) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Invited talk at drug design meeting which led to new connections in industry and academia and with postgraduate students. |
Year(s) Of Engagement Activity | 2017 |
Description | Invited talk at Institute of Infection and Global Health, University of Liverpool away day |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Other audiences |
Results and Impact | Hannah Rose Vineer presented a summary of BUG model development progress to date (talk title: Predicting parasite infections in UK sheep - the role of weather and farming practices). Approximately 200 University of Liverpool staff and student members attended. |
Year(s) Of Engagement Activity | 2018 |
Description | Invited talk on Sustainable parasite control at Botswana University of Agriculture and Natural Resources |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Undergraduate students |
Results and Impact | Hannah Rose Vineer presented a summary of current and ongoing research on sustainable parasite control to Animal Science undergraduates that could be applied in agricultural systems in Botswana. Undergraduates of this degree programme tend to progress as Veterinary Extension Officers in Botswana - a role that involves enaging with farmers on animal health issues. Therefore, there is potential for the information provided in this talk to cascade to farmers and improve the sustainability of parasite control measures in the country. |
Year(s) Of Engagement Activity | 2018 |
Description | Livestock Helminth Research Alliance talk on anthelmintic resistance and modelling |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Presented a summary of ongoing research on anthelmintic resistance and modelling at the Livestock Helminth Research Alliance. Presentation reminded parasitologists at international research institutions about the BUG project, and generated discussion on the role of modelling in sustainable parasite control. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.lihra.eu/ |
Description | NIH global neglected infectious diseases lecture |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited seminar at NIH NIAID, Washington; including webcast to other NIH intramural sites. |
Year(s) Of Engagement Activity | 2017 |
Description | NSA young sheep farmer training workshop |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | A workshop was held for young farmers on sustainable worm control in sheep, as part of the National Sheep Association sheep event, led by Dr Hannah Rose. 60 farmers attended and left with a better appreciation of the principles and practice of management to delay anthelmintic drug resistance. Participants reported improved knowledge and increased likelihood to implement practices to slow resistance development on their farms. |
Year(s) Of Engagement Activity | 2016 |
Description | Paraveterinary training in Malawi. |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | A series of training courses and workshops held for paravets in southern Malawi. Topics of roundworm control in cattle and small ruminants and anthelmintic resistance were covered. |
Year(s) Of Engagement Activity | 2017 |
Description | Poster SPPIRIT 2022 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Poster presentation regarding the improvement of the Teladorsagia genome, and the difference it makes to analyses. SPPIRIT 2022 gathered ECRs in parasitology from across Scotland at the Moredun Research Institute. There was a lot of discussion of posters during the day, more so than normal. |
Year(s) Of Engagement Activity | 2022 |
URL | https://sppiritnetwork.squarespace.com/news-events |
Description | Poster presentation at meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A poster was presented at a Jacques Monod Conference in Roscoff entitled "Genomic and transcriptomic changes in populations of parasitic nematode Haemonchus contortus following vaccine exposure"by Sallé G., Laing R., Cotton J.A., Holroyd N., Doyle S., Newlands G.F.J., Smith W.D., Britton C. and Devaney E. |
Year(s) Of Engagement Activity | 2016 |
Description | Poster presentation at meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | A poster was present by a PhD student associated with the BUG project at the annual AHDB research meeting. The audience here is varied both academic, policy makers and commercial. |
Year(s) Of Engagement Activity | 2016 |
Description | Presentation at COMBAR COST Action |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A talk was presented describing the role of social sciences in influencing farmer behaviour with respect to anthelmintic resistance |
Year(s) Of Engagement Activity | 2018 |
Description | Presentation at Graduate Women Scotland meeting by PhD student associated with BUG |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | PhD student presented a talk to Graduate Women Scotland meeting describing aims of project |
Year(s) Of Engagement Activity | 2016 |
Description | Presentation to Barcelona Institute for Global Health |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited talk to the Barcelona Institute for Global Health and STOP Consortium working group, which generated a lot of discussion and interest in using genomics to survey parasites. |
Year(s) Of Engagement Activity | 2021 |
Description | Recurring farmer training visits Botswana |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Repeated visits to farmers in Botswana every month to support ongoing data collection ad reinforce training. Teams include postgraduate students. Extends to demonstration and training of wider groups, through public meetings and farmer collectives, outside the core research areas in N and W Botswana. Accompanied on occasion by veterinary extension personnel, NGO staff involved in farmer extension, and policy makers from department of veterinary services. Includes training in parasitology and evaluation of anthelmintic drug efficacy. |
Year(s) Of Engagement Activity | 2020 |
Description | Resistance Day at Glasgow University |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | I coordinated a workshop at the Glasgow University on the Evolution and Ecology of Resistance to Biocides. Speakers were drawn from different research areas in the University with one international speaker from ETH Zurich. |
Year(s) Of Engagement Activity | 2016 |
Description | Running to stand still; Barcelona |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | This was a workshop held under the auspices of BBSRC to discuss the evolution of drug resistance in healthcare and agriculture |
Year(s) Of Engagement Activity | 2016 |
Description | SCOPS (Sustainable Control of Parasies in Sheep) technical manual for veterinarians |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Hannah Rose Vineer and Dave Bartley contributed to the 5th edition of the SCOPS technical manual for veterinarians. The manual provides guidelines on the sustainable control of parasites in sheep to limit the development of anthelmintic resistance. As a result of this update, veterinarians, anthelmintic prescribers and farmers will benefit from an improved understanding of the factors driving the development of resistance and recommended parasite control strategies, underpinned by the latest research. The manual is available through the SCOPS website, which is visited by up to 2000 unique visitors per week. |
Year(s) Of Engagement Activity | 2019,2020,2021 |
URL | https://www.scops.org.uk/advisers-technical-info/ |
Description | SCOPS stand at NSA national sheep event |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | Dr Hannah Rose assisted at the SCOPS (sustainable control of parasites in sheep) stand at the NSA (national sheep association) national sheep event in June 2016. SCOPS manned a stand at which farmers and other attendees could obtain information on, and discuss, parasite control in sheep. Correct practical drenching technique was shown in a series of live demonstrations attended by total audience of several hundred. Outcomes were greater appreciation by farmers of correct treatment practices and increased knowledge of sustainable control principles and methods. |
Year(s) Of Engagement Activity | 2016 |
Description | SCOPS steering group |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Dr Hannah Rose and Dr Eric Morgan reported by invitation to the SCOPS (sustainable control of parasites in sheep) steering group on using predictive models to target anthelmintic treatments and so reduce selection pressure for resistance through inappropriate use. A refined decision support tool for Nematodirus treatment was presented and dissemiantion plans for 2017 made, and extension of a similar approach to Haemonchus was discussed. Plans were made to deliver decision support tools for farmers, and to incorporate these tools into current best practice recommendations and policy. |
Year(s) Of Engagement Activity | 2017 |
Description | School Visit |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Visited primary school as part of Schools Science Week to inform on parasitic infections which stimulated interest and discussion. |
Year(s) Of Engagement Activity | 2019 |
Description | Seminar presentation at Charles University Department of Parasitology |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | Gave a talk (remotely due to Covid pandemic) in the Department of Parasitology seminar series at Charles University, Prague, on whole-genome approaches to studying anthelminthic resistance. Audience is a mixture of undergraduate, Masters and PhD students as well as a few faculty members. Talk followed by zoom discussion, |
Year(s) Of Engagement Activity | 2020 |
Description | Short presentation by request, with discussion |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | Roughly 30-40 farmers and others related to the industry attended a breeders meeting. One of the main articles for discussion was the use of faecal egg counts and IgA saliva as part of a rams estimated breeding value score. I was asked to give a short 20-30 minute presentation on strongyle egg counts and the use of IgA saliva to score for phenotypic resistance to parasitic nematodes, in particular T. circumcincta. The presentation stimulated a lively discussion and prompted further questions and debate afterwards. Following the meeting several farmers (in addition to those already doing so) submitted faecal samples for speciation of strongyles to Signet, who are responsible for generating these estimated breeding values. |
Year(s) Of Engagement Activity | 2017 |
Description | Short talk - institute and lab talks. Also additional talks/presentations at small meetings relating to the BUG project. |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | Short presentations are given each year to other members of the institute and/or lab group. These include at least one yearly presentation to the institute on the research carried out as part of the PhD. These talks generally stimulate questions from the audience, and are useful in generating feedback and ideas for continuing analyses and research tasks within the PhD. |
Year(s) Of Engagement Activity | 2016,2017,2018 |
Description | Short talk at Meiosis and Beyond Workshop, Earlham Institute |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Other audiences |
Results and Impact | Attended and presented at a workshop focused on the study of meiosis. The aim was to gather a diverse group of researchers to identify key themes and technological challenges, with the intention to write an opinion / perspective manuscript on the subject. A skeleton draft of a manuscript was outlined on the day. I presented some aspects of data generated from the BUG project in a talk entitled "Construction of a genome-resequencing based genetic map reveals sporadic polyploidy in an outbred parasitic nematode". |
Year(s) Of Engagement Activity | 2018 |
URL | http://www.earlham.ac.uk/meiosis-and-beyond |
Description | Short talk at conference Anthelmintic Resistance III |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A short talk was given at the international conference 'Anthelmintic Reisistance III' in Florida. Those present included researchers, postgraduate students and industry professionals. The talk described preliminary results from recent work using genome wide sequencing to identify genomic regions of selection following ivermectin treatment in a UK field population of T. circumcincta. |
Year(s) Of Engagement Activity | 2018 |
Description | Short talk given to parasitologists within the University of Glasgow |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Other audiences |
Results and Impact | Gave a 25 minute talk about the research methods and current findings from the project to other parasitologists within the institute at a 'Research Update Meeting'. These meetings are very well attended by parasitologists from a wide range of disciplines. |
Year(s) Of Engagement Activity | 2019 |
Description | Synergy Farm Animal Health Haemonchus event |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Hannah Rose Vineer led a short workshop on "Haemonchus and the weather" for farmers, vets and pharmaceutical industry representatives. The workshop was repeated several times over the course of the evening, to a total of approximately 120 people. The workshop generated discussion on how the weather impacts gastrointestinal nematodes and the way they are controlled, and highlighted the activities of UK research institutions, especially the BUG consortium. |
Year(s) Of Engagement Activity | 2019 |
URL | http://synergyfarmhealth.com/event/haemonchus-open-evening/ |
Description | Talk at World Association for the Advancement of Veterinary Parasitology (WAAVP) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Gave a talk at World Association for the Advancement of Veterinary Parasitology (WAAVP) |
Year(s) Of Engagement Activity | 2021 |
Description | Talk at the British Association for the Advancement of Veterinary Parasitology Winter meeting, 10-11 December 2019, Cambridge |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Dr Hannah Vineer presented a talk entitled "Weather and the development of anthelmintic resistant Haemonchus contortus" |
Year(s) Of Engagement Activity | 2019 |
Description | Talk at workshop on Food Security, Parasites and Tick Borne Disease in Botswana |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Policymakers/politicians |
Results and Impact | Hannah Rose Vineer and Eric Morgan led sessions on "Weather-smart worm control", "Targeted parasite treatment" and "Weather and gastrointestinal nematode transmission" at the Botswana National Veterinary Laboratory in Gabarone, Botswana. The activities highlighted the work conducted by the BUG consortium and potential internaional applications of the research. The workshop has led to several follow-on projects, including one led by Eric Morgan on plants with anthelmintic properties (BBSRC ref: BB/S014748/1) and a pilot studies of ticks and tick-borne disease in the region led by Hannah Rose Vineer. The contacts made during the workshop provide an international route to impact for tools generated as a result of the BUG project. |
Year(s) Of Engagement Activity | 2018 |
Description | The BUG consortium |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Presented a poster at COMBAR, an EU COST Action on anthelmintic resistance |
Year(s) Of Engagement Activity | 2018 |
Description | Three minute thesis talk |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | I gave a talk as part of the three minute thesis (3MT) competition at the University of Glasgow. There were about 25 people in the room. The talk was filmed, tweeted about by the 3MT group and others and will be loaded onto the University of Glasgow website in the future. The talk covered the reason behind the PhD and briefly mentioned the methods, and future outcomes. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.gla.ac.uk/research/ourresearchenvironment/prs/pgrcoursesandevents/threeminutethesiscompe... |
Description | Twitter account 'SheepwormPhD' |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Twitter account set up primarily to reach farmers and vets for knowledge transfer and knowledge exchange. Currently the account has 96 followers, most of whom are within the UK and 13 of whom are new from the last 30 days. The most common word in their profiles is 'sheep'. Most tweets are either re-tweets of industry relevant information, or tweets about sheep/parasite talks or other activities related to work carried out as part of the PhD or wider BUG consortium project. Tweets in the last mon |
Year(s) Of Engagement Activity | 2018,2019 |
URL | https://twitter.com/sheepwormPhD |
Description | Two prison visits to provide a teaching session to prison learners. |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | On two separate occasions I went with Cell Block Science (https://news.st-andrews.ac.uk/archive/cell-block-science/) into HMP Shotts and HMP Low Moss to provide a session about sheep parasites and anthelmintic resistance to the prison learners. At each prison two sessions were run, reaching between about 6-10 learners at each session. A combination of powerpoint, discussion, and in-depth activities were used to stimulate learning and problem solving. Learners were taken from the concept of the disease understudy in this PhD, through the complexities of the nature of the disease, to actively planning with farm maps, anthelmintic efficacy data, species data and farm management data how they would manage the disease on their farm. Some learners were from a farming, or animal health background which meant that the information was directly applicable to them and should make a lasting impression for when they leave prison. All learners were actively engaged in the sessions and from the activities performed it was clear that the impact had been achieved. |
Year(s) Of Engagement Activity | 2018 |
URL | https://twitter.com/sheepwormPhD/status/1060914897440120841 |
Description | Webinars on parasite control |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | Repeated webinars to farmers and professional advisors, starting with Farmer's Guardian 'beat the parasites' series. So far two webinars attended by a total of 250 farmers, vets and allied professionals. |
Year(s) Of Engagement Activity | 2020 |
Description | Workshop for farmers on sustainable parasite control |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Hannah Rose Vineer led a workshop on "Sustainable parasite control in livestock Focus on: Gutworms" to farmers for the Dartmoor Hill Farm Project. The workshop generated discussion on sustainable control of gastrointestinal nematodes in the region, encouraged the group to work together to problem-solve and identify more sustainable approaches to anthelmintic usage, and highlighted the activities of UK research institutions, especially the BUG consortium. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.dartmoorhillfarmproject.co.uk/ |
Description | Workshop on worm control |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Industry/Business |
Results and Impact | Within a workshop on best practice in worm control in sheep, which was funded by a SG project the audience was made aware of the BUG project and its goals. |
Year(s) Of Engagement Activity | 2017 |
Description | Workshops for BBSRC Resistance highlight call |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | I presented at two workshops to accompany the BBSRC Highlight call in Resistance in Agriculture |
Year(s) Of Engagement Activity | 2016 |
Description | keynote presentation, ICOPA 2022. |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | invited keynote presentation mostly on BUG results on "Genomic insights into anthelminthic drug resistance in Haemonchus contortus" at the International Congress on Parasitology, Copenhagen. |
Year(s) Of Engagement Activity | 2022 |
Description | teaching and facilitating at IUIS-FAIS-IMMUNO-ETHIOPIA 2020 course, Bahir Dar, Ethiopia |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Taught a number of topics in parasite genetics/genomics at a workshop on parasitology and immunology, to a group of postgraduate students from across Africa. This included some teaching on anthelminthics, as an example of an application of whole-genome sequencing. |
Year(s) Of Engagement Activity | 2020 |
URL | https://www.immunopaedia.org.za/online-courses/iuis-courses/iuis-fais-immuno-ethiopia-2020/ |