Nuclear calcium regulation of plant development
Lead Research Organisation:
John Innes Centre
Department Name: Cell and Develop Biology
Abstract
Calcium signalling is essential for growth and development, in both plants and animals. In animals nuclear calcium release is a potent regulator of neuronal gene expression and of cell proliferation. Nuclear calcium signalling is also known to be essential in legumes to promote associations with nitrogen fixing bacteria and phosphate delivering arbuscular mycorrhizal fungi. Legumes are among the world's most important agricultural food crops that are beneficial to billions of farmers and consumers worldwide and provide an essential aspect of natural soil enrichment of organic nitrogen compounds.
The mechanisms of plant nuclear calcium signalling were poorly understood. During my work I have used the symbiotic associations in legumes as a platform to dissect plant nuclear calcium signalling. Using a wide range of approaches, I discovered a number of ion channels located at the nuclear envelope that are responsible for symbiotic nuclear calcium release. Among them, I defined the first plant nuclear-associated calcium channels encoded by cyclic nucleotide gated channels (CNGC15s). The CNCG15s sit at the nuclear envelope in a complex with a potassium permeable channel (DMI1), also required for the generation of the symbiotic nuclear calcium signals. Interestingly, CNGC15s and DMI1 are conserved across all land plants, including non-symbiotic species, strongly suggesting that they have other functions during plant development. Consistent with this I have found a number of defects in Arabidopsis lines mutated in CNGC15 and DMI1, that include root developmental defects. I propose that my research in legumes has revealed the generic plant machinery involved in the regulation of nuclear calcium release. By studying the components that regulate nuclear calcium release I will be able to understand when and where nuclear calcium signalling is important.
My proposal will focus on the role of nuclear calcium signalling during root development, both root growth and associations with symbiotic microorganisms. Consistent with a function for CNGC15 and DMI1 in root development I have observed nuclear calcium responses in Arabidopsis root meristematic cells during their response to the phytohormones auxin and cytokinin. These calcium responses are mechanistically different from the nuclear calcium signals observed in legumes during symbiotic associations. My proposed research integrates molecular biology, genetics, cell biology, chemistry, electrophysiology and mathematical modelling to investigate how CNGC15-DMI1 regulates nuclear calcium release leading to plant developmental processes. It will use a large collection of Arabidopsis mutant and transgenic lines, that I have already generated, with a panel of nuclear calcium sensors allowing detection of nuclear calcium signals in an array of Arabidopsis mutants. My work will dissect the functions that nuclear calcium signalling plays in root developmental processes and how diverse nuclear calcium signals are encoded. Finally, through a combination of transcriptomics and mutant screens, I will be uniquely poised to decipher the downstream signalling components associated with nuclear calcium signalling during root development.
The mechanisms of plant nuclear calcium signalling were poorly understood. During my work I have used the symbiotic associations in legumes as a platform to dissect plant nuclear calcium signalling. Using a wide range of approaches, I discovered a number of ion channels located at the nuclear envelope that are responsible for symbiotic nuclear calcium release. Among them, I defined the first plant nuclear-associated calcium channels encoded by cyclic nucleotide gated channels (CNGC15s). The CNCG15s sit at the nuclear envelope in a complex with a potassium permeable channel (DMI1), also required for the generation of the symbiotic nuclear calcium signals. Interestingly, CNGC15s and DMI1 are conserved across all land plants, including non-symbiotic species, strongly suggesting that they have other functions during plant development. Consistent with this I have found a number of defects in Arabidopsis lines mutated in CNGC15 and DMI1, that include root developmental defects. I propose that my research in legumes has revealed the generic plant machinery involved in the regulation of nuclear calcium release. By studying the components that regulate nuclear calcium release I will be able to understand when and where nuclear calcium signalling is important.
My proposal will focus on the role of nuclear calcium signalling during root development, both root growth and associations with symbiotic microorganisms. Consistent with a function for CNGC15 and DMI1 in root development I have observed nuclear calcium responses in Arabidopsis root meristematic cells during their response to the phytohormones auxin and cytokinin. These calcium responses are mechanistically different from the nuclear calcium signals observed in legumes during symbiotic associations. My proposed research integrates molecular biology, genetics, cell biology, chemistry, electrophysiology and mathematical modelling to investigate how CNGC15-DMI1 regulates nuclear calcium release leading to plant developmental processes. It will use a large collection of Arabidopsis mutant and transgenic lines, that I have already generated, with a panel of nuclear calcium sensors allowing detection of nuclear calcium signals in an array of Arabidopsis mutants. My work will dissect the functions that nuclear calcium signalling plays in root developmental processes and how diverse nuclear calcium signals are encoded. Finally, through a combination of transcriptomics and mutant screens, I will be uniquely poised to decipher the downstream signalling components associated with nuclear calcium signalling during root development.
Technical Summary
Root legume symbioses are the only plant system with a well-defined nuclear calcium signal. I have used this system to dissect nuclear calcium signalling in plants. I demonstrated the first nuclear calcium channels in plants, encoded by cyclic nucleotide gated channels (CNGC)15s, which form a complex with the potassium permeable channel, DMI1, to create a large channel complex capable of generating symbiotic nuclear calcium oscillations. These channels are conserved across many plant species, including non-symbiotic plants, and from this I concluded that this channel complex must play additional functions beyond the establishment of symbiotic associations. To assess this, I generated Arabidopsis mutants in DMI1 and CNGC15 in Arabidopsis. Mutations in Arabidopsis CNGC15 show defects in root development and fertility, as well as other defects yet to be validated. Interestingly Arabidopsis dmi1 mutants also show root developmental defects, although the effects are opposite to those observed in cngc15. These preliminary analyses imply that the channel complex that I discovered in legumes plays broader roles in plant development. I propose that this channel complex controls all aspects of nuclear calcium signalling in plants and thus through these analyses, I will be able to define where and when nuclear calcium signalling is important during plant development and environmental responses. My fellowship proposal focuses on the role of CNGC15 and DMI1 during root development, both root growth and symbiotic associations in legumes. I have demonstrated that phytohormones activate nuclear calcium responses in Arabidopsis root meristematic cells. In my proposed fellowship I will dissect the detailed mechanism of action of the DMI1/CNGC15 channel complex and its broader function during root development.
Planned Impact
The outcomes of the proposed research will be of significant benefit to farmers and plant breeders as it has the potential to impact on crop yield and thereby to the UK public in general by contributing to UK's economic competitiveness.
Over 800 million people lack adequate access to safe and nutritious food. The world faces an even greater crisis in food security as expected global population growth to over 9 billion by 2050, is coupled with global climate change. On the issue of global warming, we have underestimated extreme climate fluctuations at the decadal time scale, which will influence UK and worldwide food security by creating unpredictable food shocks. It is urgent to develop new strategies to sustainably enhance worldwide agricultural production. Thus, understanding plant development, and how plants can integrate diverse environmental stimuli into developmental responses, is essential to improve resilience in crop production. My work will establish a molecular underpinning to the understanding of root development, a strategic important research area.
Calcium (Ca2+) is a universal regulatory molecule and intimately couples primary biotic and abiotic signals to many cellular processes allowing plant development and adaptation to environmental changes. My proposed research is at the leading edge of studies in plant development and nuclear Ca2+ signalling, providing exciting, new insights into the mechanisms of nuclear Ca2+ signal transduction during plant development. I will unravel the regulatory mechanisms of the central regulator of nuclear Ca2+ signal, which is at the core of multiple developmental processes such as root growth and fertility, targets for plant breeders to improve crop production.
By elucidating the mechanism of plant nuclear Ca2+ generation, this proposed research will position the UK as the leader in a new research area. Beyond the excellence, the outcome of this proposed research, combined with the new technology of genome editing, has the potential to be directly transferred to the main crops constituting the world's breadbasket (eg. wheat, maize) and major crops in developing countries (eg. rice, millet, sorghum). This could impact the production at multiple levels, from the biggest farming regions in UK, USA and Russia to the small farmers in sub-Saharan Africa.
In summary, the proposed project opens a new research area in plant sciences by determining how nuclear Ca2+ signals are regulated to influence plant growth. This research will have strong social, economic impacts involving UK competitiveness and global food security.
Over 800 million people lack adequate access to safe and nutritious food. The world faces an even greater crisis in food security as expected global population growth to over 9 billion by 2050, is coupled with global climate change. On the issue of global warming, we have underestimated extreme climate fluctuations at the decadal time scale, which will influence UK and worldwide food security by creating unpredictable food shocks. It is urgent to develop new strategies to sustainably enhance worldwide agricultural production. Thus, understanding plant development, and how plants can integrate diverse environmental stimuli into developmental responses, is essential to improve resilience in crop production. My work will establish a molecular underpinning to the understanding of root development, a strategic important research area.
Calcium (Ca2+) is a universal regulatory molecule and intimately couples primary biotic and abiotic signals to many cellular processes allowing plant development and adaptation to environmental changes. My proposed research is at the leading edge of studies in plant development and nuclear Ca2+ signalling, providing exciting, new insights into the mechanisms of nuclear Ca2+ signal transduction during plant development. I will unravel the regulatory mechanisms of the central regulator of nuclear Ca2+ signal, which is at the core of multiple developmental processes such as root growth and fertility, targets for plant breeders to improve crop production.
By elucidating the mechanism of plant nuclear Ca2+ generation, this proposed research will position the UK as the leader in a new research area. Beyond the excellence, the outcome of this proposed research, combined with the new technology of genome editing, has the potential to be directly transferred to the main crops constituting the world's breadbasket (eg. wheat, maize) and major crops in developing countries (eg. rice, millet, sorghum). This could impact the production at multiple levels, from the biggest farming regions in UK, USA and Russia to the small farmers in sub-Saharan Africa.
In summary, the proposed project opens a new research area in plant sciences by determining how nuclear Ca2+ signals are regulated to influence plant growth. This research will have strong social, economic impacts involving UK competitiveness and global food security.
Organisations
People |
ORCID iD |
Myriam Charpentier (Principal Investigator / Fellow) |
Publications
Thorpe SD
(2017)
Highlight on the dynamic organization of the nucleus.
in Nucleus (Austin, Tex.)
Charpentier M
(2018)
Calcium Signals in the Plant Nucleus: Origin and Function.
in Journal of experimental botany
Kelner A
(2018)
Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells.
in Frontiers in plant science
Leitão N
(2019)
Nuclear calcium signatures are associated with root development.
Leitão N
(2019)
Nuclear calcium signatures are associated with root development.
in Nature communications
Newman-Griffis AH
(2019)
Medicago LINC Complexes Function in Nuclear Morphology, Nuclear Movement, and Root Nodule Symbiosis.
in Plant physiology
Zuccaro A
(2020)
Breeding for resistance: can we increase crop resistance to pathogens without compromising the ability to accommodate beneficial microbes?
in The New phytologist
Jacott CN
(2020)
Mildew Locus O facilitates colonization by arbuscular mycorrhizal fungi in angiosperms.
in The New phytologist
Thor K
(2020)
The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity.
in Nature
Description | 1- We have explored the potential of single fluorescent protein-based Ca2+ sensors, the GECOs, for multicolor and simultaneous imaging of the spatiotemporal dynamics of cytoplasmic and nuclear Ca2+ signaling in root cells. Single and dual fluorescence nuclear and cytoplasmic-localized GECOs expressed in transgenic Medicago truncatula roots and Arabidopsis thaliana were used to successfully monitor Ca2+ responses to microbial biotic and abiotic elicitors. In M. truncatula, we demonstrate that GECOs detect symbiosis-related Ca2+ spiking variations with higher sensitivity than the yellow FRET-based sensors previously used. Additionally, in both M. truncatula and A. thaliana, the dual sensor is now able to resolve in a single root cell the coordinated spatiotemporal dynamics of nuclear and cytoplasmic Ca2+ signaling in vivo. The GECO-based sensors presented here therefore represent powerful tools to monitor Ca2+ signaling dynamics in vivo in response to different stimuli in multi-subcellular compartments of plant cells. 2- We show that nuclear Ca2+ signalling initiates in the nucleus of Arabidopsis root cells and that these signals are correlated with primary root development, including meristem development and auxin homeostasis. In addition, we demonstrate that altering genetically AtDMI1 is sufficient to modulate the nuclear Ca2+ signatures, and primary root development. This finding supports the postulate that stimulus-specific information can be encoded in the frequency and duration of a Ca2+ signal and thereby regulate cellular function. 3- We combined proteomics and engineering approaches to demonstrate that the calcium-bound form of the calmodulin 2 (CaM2) associates with CYCLIC NUCLEOTIDE GATED CHANNEL 15 (CNGC15s), closing the channels and providing the negative feedback to sustain the oscillatory mechanism. We further unraveled that the engineered CaM2 accelerates early endosymbioses and enhanced root nodule symbiosis but not arbuscular mycorrhization. 4- We characterize a novel mutant allele of AtCNGC15 and demonstrate that the mutation of a highly conserved aspartic acid in the C-linker domain is sufficient to impair the gating of AtCNCG15. We demonstrate that AtCNGC15 mediates the nuclear calcium release that modulates root apical meristem development and nitrate- induced LBD39 expression. We also show that, in the presence of nitrate, the relocalization of AtCNGC15 at the plasma membrane occurs specifically in the columella cells. Our results further suggest that the induction of LBD37, LBD38 and LBD39 in presence of nitrate is modulated by different inputs of cytoplasmic or nuclear calcium release. |
Exploitation Route | too early to say |
Sectors | Agriculture Food and Drink Environment |
URL | https://www.ncbi.nlm.nih.gov/pubmed/31653864 |
Description | H2020-MSCA-IF-2019 |
Amount | € 212,933 (EUR) |
Funding ID | 891144 |
Organisation | European Commission |
Sector | Public |
Country | European Union (EU) |
Start | 05/2020 |
End | 05/2022 |
Description | NRP-DTP |
Amount | £79,108 (GBP) |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2018 |
End | 09/2022 |
Description | NRP-DTP |
Amount | £79,108 (GBP) |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2019 |
End | 09/2023 |
Description | NRP-DTP |
Amount | £79,108 (GBP) |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2020 |
End | 09/2024 |
Title | Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells |
Description | Spatiotemporal changes in cellular calcium (Ca2+) concentrations are essential for signal transduction in a wide range of plant cellular processes. In legumes, nuclear and perinuclear-localized Ca2+ oscillations have emerged as key signatures preceding downstream symbiotic signaling responses. Förster resonance energy transfer (FRET) yellow-based Ca2+ cameleon probes have been successfully exploited to measure the spatiotemporal dynamics of symbiotic Ca2+ signaling in legumes. Although providing cellular resolution, these sensors were restricted to measuring Ca2+ changes in single subcellular compartments. In this study, we have explored the potential of single fluorescent protein-based Ca2+ sensors, the GECOs, for multicolor and simultaneous imaging of the spatiotemporal dynamics of cytoplasmic and nuclear Ca2+ signaling in root cells. Single and dual fluorescence nuclear and cytoplasmic-localized GECOs expressed in transgenic Medicago truncatula roots and Arabidopsis thaliana were used to successfully monitor Ca2+ responses to microbial biotic and abiotic elicitors. In M. truncatula, we demonstrate that GECOs detect symbiosis-related Ca2+ spiking variations with higher sensitivity than the yellow FRET-based sensors previously used. Additionally, in both M. truncatula and A. thaliana, the dual sensor is now able to resolve in a single root cell the coordinated spatiotemporal dynamics of nuclear and cytoplasmic Ca2+ signaling in vivo. The GECO-based sensors presented here therefore represent powerful tools to monitor Ca2+ signaling dynamics in vivo in response to different stimuli in multi-subcellular compartments of plant cells. |
Type Of Material | Physiological assessment or outcome measure |
Year Produced | 2018 |
Provided To Others? | Yes |
Impact | the cytoplasmic and nucleoplasmic dual sensors developed in this work are powerful tools to analyze the subcellular Ca2+ dynamics between the cytoplasm and nucleus in planta. These sensors will unequivocally be of interest in exploring Ca2+-mediated signaling in response to diverse stimuli in different plant species. |
URL | https://www.frontiersin.org/articles/10.3389/fpls.2018.00245/full |
Description | Characterization of the role of clade IV MLO in arbuscular mycorrhization |
Organisation | Center for Excellence in Molecular Plant Sciences |
Country | China |
Sector | Academic/University |
PI Contribution | We provided our expertise in arbuscular mycorrhiza phenotyping, mutant characterization and phylogenetic analysis. |
Collaborator Contribution | The Ridout lab provided expertise in barley and wheat genetics and the Murray lab in transcriptomics analyses. |
Impact | Publication: Mildew Locus O facilitates colonization by arbuscular mycorrhizal fungi in angiosperms. Jacott CN, Charpentier M, Murray JD, Ridout CJ. New Phytol. 2020 Jul;227(2):343-351. doi: 10.1111/nph.16465. |
Start Year | 2017 |
Description | Characterization of the role of clade IV MLO in arbuscular mycorrhization |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We provided our expertise in arbuscular mycorrhiza phenotyping, mutant characterization and phylogenetic analysis. |
Collaborator Contribution | The Ridout lab provided expertise in barley and wheat genetics and the Murray lab in transcriptomics analyses. |
Impact | Publication: Mildew Locus O facilitates colonization by arbuscular mycorrhizal fungi in angiosperms. Jacott CN, Charpentier M, Murray JD, Ridout CJ. New Phytol. 2020 Jul;227(2):343-351. doi: 10.1111/nph.16465. |
Start Year | 2017 |
Description | Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis |
Organisation | Ohio State University |
Country | United States |
Sector | Academic/University |
PI Contribution | Supervision of the study Symbioses experiments |
Collaborator Contribution | Study of the LINC components |
Impact | Newman-Griffis AH, Del Cerro P, Charpentier M, Meier I. Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis. Plant Physiology. 2019. 179(2):491-506 |
Start Year | 2016 |
Description | Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells |
Organisation | French National Institute of Agricultural Research |
Department | INRA Toulouse |
Country | France |
Sector | Public |
PI Contribution | We generated the Dual calcium sensor and the stably transformed plants (Arabidopsis thaliana and Medicago truncatula) We performed the research in Arabidopsis thaliana |
Collaborator Contribution | The Carvalho-Niebel lab generated DUAL sensor and stably transformed Medicago truncatula Performed the experiment in Medicago truncatula |
Impact | Kelner A, Leitao N, Chabaud M, Charpentier M*, De Carvalho-Niebel F*. Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells. Frontiers in Plant Science. 2018. 9:245. |
Start Year | 2017 |
Description | The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity |
Organisation | University of Zurich |
Country | Switzerland |
Sector | Academic/University |
PI Contribution | We provided our expertise in ion channel, calcium recording and use of heterologous system to assess calcium permeability. |
Collaborator Contribution | The Zipfel lab identified the OSCA1.3 ion channel as a target phosphorylated by BIK1 and characterized its role in plant stomatal immunity |
Impact | Publication: The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Thor K, Jiang S, Michard E, George J, Scherzer S, Huang S, Dindas J, Derbyshire P, Leitão N, DeFalco TA, Köster P, Hunter K, Kimura S, Gronnier J, Stransfeld L, Kadota Y, Bücherl CA, Charpentier M, Wrzaczek M, MacLean D, Oldroyd GED, Menke FLH, Roelfsema MRG, Hedrich R, Feijó J, Zipfel C. Nature. 2020 Sep;585(7826):569-573. doi: 10.1038/s41586-020-2702-1. |
Start Year | 2017 |
Description | Advances - The john Innes Centre magazine |
Form Of Engagement Activity | A magazine, newsletter or online publication |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Getting to the root of agricultural pollution. |
Year(s) Of Engagement Activity | 2021 |
Description | Agri-tech: Science in Sustainable Agriculture |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | The talk describe the urgency to shift our agricultural practice and reduce chemical fertilizer use. The talk was well received and opened new connection with industries. |
Year(s) Of Engagement Activity | 2021 |
Description | Annual Science Meeting for general audience organised by the research and support Staff Voice |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | The talk was well attended, with 40 people attending in person and 172 registrations in total. Four local (Norfolk) schools attended online including Years 10 and 13 students from Wymondham College, Notre Dame, Sprowston High and Sheringham High. There were also 20 people from TSL and Youth STEMM and feedback from the schools was very positive. The hybrid format had been very successful. It is a great way to introduce school students to JIC science. |
Year(s) Of Engagement Activity | 2021 |
Description | Co-organiser of the satellite meeting on Signal Transduction Pathways in Nitrogen-Fixing Symbioses |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | The meeting focused on molecular, genetic and in vivo dynamic aspects of plant symbiotic signaling, and offered the possibility to highlight the recent progress in Nitrogen-fixing signal transduction. This satellite meeting provided a privileged frame for presentations, discussions and exchanges around the latest scientific discoveries in plant signal transduction related to Nitrogen-fixing symbiosis, that were not covered during the 13rd European Nitrogen Fixation Conference (ENFC) in Stockholm, Sweden. |
Year(s) Of Engagement Activity | 2018 |
URL | http://enfc2018.org/ |
Description | Co-organization of the plant symbioses virtual seminar |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | The virtual seminar series occur every two months since the start of the COVID19 pandemic. The virtual seminar series aim at gathering the students, postdoctoral scientist, scientists and journal editors to stay connected and have an overview of the latest discoveries in Plant symbioses. |
Year(s) Of Engagement Activity | 2020,2021 |
Description | European Calcium society webinar ; Invited speaker |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | The talk was well received and open a debate on the differences and similarities between plants and animal calcium signalling. |
Year(s) Of Engagement Activity | 2021 |
Description | Exhibition |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Artwork created by Henry Driver and commissioned by Essex County Council to promote that food and the natural elements which sustain its' production, such as ample water & healthy soil should be cherished and regenerated, not wasted and degraded. |
Year(s) Of Engagement Activity | 2020 |
URL | http://www.henrydriverartist.com/regenerate.html |
Description | Invited Speaker: Host Microbe Interactions- Norwich Research Park Group |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | Discussion within the Norwich research park scientists working on Host-Microbe Interactions in plant and animal. |
Year(s) Of Engagement Activity | 2018 |
Description | Invited plenary speaker: 13th European Nitrogen Fixation Conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Prolific discussions and interest in nitrogen fixation signaling, as well as in promoting equality and diversity in science |
Year(s) Of Engagement Activity | 2018 |
Description | Invited seminar at the 3rd Adam Kondorosi Symposium: Frontiers in Beneficial Plant Microbe Interactions |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | The objective of this Symposium was to create a scientific event that is at the forefront of fundamental research in beneficial plant-microbe interactions. The Symposium brought together about 150 participants in a rather informal atmosphere, facilitating exchanges. This promoted exciting discussion with researchers - in particular those at the early stage of their career. |
Year(s) Of Engagement Activity | 2017 |
URL | https://symposium.inra.fr/ak-ips2-2017_eng/ |
Description | Invited speaker at the GARNet workshop on Advances in Plant Imaging, Warwick |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | A lecture on Calcium imaging was given to a research group interested in Plant Imaging. The positive outcome was demonstarted by an increase number of scientists looking forward to collaborate or to gain further experience in this area. |
Year(s) Of Engagement Activity | 2019 |
URL | https://garnetcommunity.org.uk/node/988 |
Description | Invited speaker at the XIX International Botanical Congress, Shenzhen, China |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Undergraduate students |
Results and Impact | Invited speaker at on Calcium transport and signaling. |
Year(s) Of Engagement Activity | 2017 |
Description | Invited speaker at the XVIII International Plant Microbe Interaction conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | The presentation was well received and led to new collaborations. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.ismpmi.org/Congress/2019/Pages/default.aspx |
Description | Invited speaker: 18th International Workshop on Plant Membrane Biology - Glasgow, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Our research outcome was well received, which sparked questions and discussion afterwards. |
Year(s) Of Engagement Activity | 2019 |
Description | Invited speaker: 6th International Conference on Plant Vascular Biology |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | collaboration and networking |
Year(s) Of Engagement Activity | 2022 |
URL | https://www.pvb2022.org/ |
Description | Invited speaker: Gordon Research Conference on Organellar Channels and Transporters, Mount Snow, USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | This dedicated conference provided a unique opportunity for animal and plant scientists working on different organelles to share data and ideas to help addressing current challenges of organelle biology, foster collaborations, and identify new ways to treat common and rare diseases based on better understanding of mechanisms of ion/metabolite transport in various organelles. |
Year(s) Of Engagement Activity | 2017 |
Description | Invited speaker: International Plant Calcium signalling conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | collaboration and networking |
Year(s) Of Engagement Activity | 2022 |
Description | Invited speaker: International Plant Molecular Biology Conference, Montpellier, France |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | stimulated discussion promoting new collaboration. |
Year(s) Of Engagement Activity | 2018 |
Description | Invited speaker: International symposium on "Plant cell compartments: stay connected" |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | collaboration, networking |
Year(s) Of Engagement Activity | 2022 |
Description | Invited speaker: Max Planck Institute for Plant Breeding Research, Cologne, Germany |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Exciting discussion promoting new collaboration |
Year(s) Of Engagement Activity | 2019 |
Description | Invited speaker: Max Planck Institute of Molecular Plant Physiology, Golm, Germany |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | Discussion with scientists to increase collaborative effort aiming at improving crop production |
Year(s) Of Engagement Activity | 2018 |
Description | Invited speaker: SEB Functional organisation of the nuclear periphery, Florence, Italy |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | This session included presentations on recent advances in knowledge and understanding molecular functions, dynamics and interactions of proteins of the nuclear envelope across kingdoms; topics will include the proteins of the linker of cytoskeleton and nucleoskeleton complex; the nuclear pore complex; the nucleoskeleton and lamina and the interactions of the nuclear envelope with the cytoskeleton. It included applications ranging from the role of nuclear envelope proteins in plant stress and disease responses to developmental disorders caused by laminopathies. The session generated very stimulated discussions. |
Year(s) Of Engagement Activity | 2018 |
Description | Organization of the International Plant calcium signalling conference 2017 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | This conference brought all the plant calcium community together. It triggered very stimulating and interesting discussions and new opportunities of collaboration for many attendees. |
Year(s) Of Engagement Activity | 2017 |
URL | http://plantcalciumsignalling.com/ |
Description | Plant calcium signalling conference; Invited speakers |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited speaker at the international Plant calcium signalling conference organized via zoom due to covid19. The talk was well received and several student, post-doc and group leaders debated with me afterward. |
Year(s) Of Engagement Activity | 2021 |
Description | Press release |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Press release to inform general public |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.jic.ac.uk/news/key-role-for-calcium-release-in-root-development/ |