DFW - Designing Future Wheat - Work package 3 (WP3) - Germplasm

Lead Research Organisation: Rothamsted Research
Department Name: Unlisted

Abstract

WP3 will accelerate the discovery and deployment of genes and alleles of high value for breeding, thus maximising impact from discoveries within WP1, WP2, and WISP and 20:20 Wheat. Tangible WP3 outputs for this purpose are referred to as 'toolkits', one composed of pre-breeding germplasm and the other the molecular markers needed to exploit that germplasm. WP3 addresses two topics. The first advances the genetic and genomic characterisation of foundation germplasm. This germplasm, together with the derived segregating populations and associated methodologies, will supply new toolkit genes, alleles, and markers. In the second the new genes, alleles and markers are validated and tested for their value in breeding. This gene discovery funnel produces packages of traits with molecular markers assembled into the two toolkits. WP3 channels new genes and alleles based on the discoveries of DFW and previous wheat programme outputs, into commercial wheat breeding. The breeders' toolkits and associated information will be improved and expanded, for the benefit of breeders and the wider plant community.

Publications

10 25 50
 
Description During the project, we have provided BBSRC with a google.doc site, which lists all DFW outputs (including data access sites, germplasm generated, datasets generated, publications, and public engagement activities). This document is updated on a regular basis. All the objectives during the DFW reporting period have been met, and many exceeded. All the committees within DFW are running including the key data coordination task force (DCTF) and Breeder's toolkit committees, both with WP3 representation.

• The DFW toolkit, including Breeders Toolkit field trialed for performance in multiple years.
• Inclusion of ten new Rht-1 dwarfing alleles in the Breeder's Toolkit.
• Optimized development of cost-effective phenotyping and phenotypic analysis
• New targets for yield, flowering time and height identified and to be proposed for the breeders toolkit. Targets relating to NUE still under investigation.
Exploitation Route further development of Breeders toolkits
Sectors Agriculture, Food and Drink

URL https://designingfuturewheat.org.uk/
 
Description further development of Breeders toolkits
First Year Of Impact 2017
Sector Agriculture, Food and Drink,Digital/Communication/Information Technologies (including Software)
Impact Types Societal,Economic

 
Title Novel wheat germplasm for manipulation of crop height and response to stress 
Description Gene Pathway Role Alleles Current Generation Phenotype GA20ox1 GA GA biosynthesis A1b.B1b.D1c BC4F3 Semidwarf, 88% of WT height; field trial 2020 GA20ox2 GA GA biosynthesis A2b.B2b.D2b BC3F3 Semidwarf, 87% of WT height; field trial 2020 GA20ox4 GA GA biosynthesis A4b.B4b.D4c BC0F2 Semidwarf GA3ox2 GA GA biosynthesis A2b.B2b.D2b BC3F3 Extreme GA-deficient dwarf GA3ox3 GA GA biosynthesis A3b.B3c BC3F3 Low grain GA levels; no effect on seed development GA1ox1 GA GA modulation B1d BC5F3 High grain GA levels; no effect on seed development GA2ox3 GA GA inactivation A3b.B3b.D3b BC3F3 7% taller than WT Cadenza at BC3F3 GA2ox4 GA GA inactivation A1b.B1b.D1b BC3F1 t.b.d. GA2ox9 GA GA inactivation A9c.B9c.D9b BC3F1 ~10% taller than WT at BC0F2 EUI-1 GA GA inactivation A1b.B1c.D1c BC3F1 Elongated upper internodes ABA8OH1 ABA ABA inactivation A1c.B1b.D1b BC2F3 Evidence of increased seed dormancy D14 SL Strigolactone receptor A1b.B1b.D1b BC3F3 High tillering semi-dwarf D3 SL SL F-box B1c.D1d BC3F3 High tillering semi-dwarf D53 SL SL effector B1c (GOF) BC0F2 No phenotype observed SPL14 SL Shoot branching B1b (GOF) BC2F1 Low tillering, thicker culms SPL17 SL Shoot branching A1b (GOF) BC0F2 No phenotype observed TB1 SL Shoot branching A1b.B1b.D1b BC3F1 tbd IPI1 SL Shoot branching A1b.B1b.D1b BC0F2 No phenotype observed 
Type Of Material Biological samples 
Year Produced 2019 
Provided To Others? Yes  
Impact progress for Giberellin genes controlling plant and grain architecture 
 
Title NAIB supply germplasm to Hohenheim University Jul 2017 
Description DFW germplasm seed request dispatched to Friedrich Longin, Hohenheim University 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to Agricultural Institute Osijek, Croatia 
Description NIAB supply 98 diverse lines to Dario Novoselovic (Agricultural Institute Osijek ) Croatia 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to Agroscope, July 2017 
Description DFW germplasm seed request dispatched to Dario Fossati, Agroscope, Switzerland 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to Cerela Inc, Canada 
Description NIAB supply 98 diverse lines to Anna Archambault (Cerela inc.) Canada 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to Hohenheim Uni, Germany 2019 
Description NIAB supply 98 diverse lines to Friedrich Longin ( Hohenhiem University) Germany 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to SENSAKO, S Africa 
Description germplasm Seed request dispatched to SENSAKO PTY in South Africa 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? No  
Impact none reported 
 
Title NIAB supply germplasm to SRUC Jul 2017 
Description DFW germplasm seed dispatched to J R Adams, SRUC 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to Sejet Plant Breeding, Denmark Sept 2019 
Description NIAB supply 98 diverse lines to Finn Borum ( Sejet Plant Breeding) Denmark 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to Uni Leicester Sept 2019 
Description NIAB supply 98 diverse lines to James Higgins (University of Leicester ) UK 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title NIAB supply germplasm to Uni. Guelph Jul 2017 
Description DFW germplasm seed requested by Ali Navabi, University of Guelph, Canada 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact none reported 
 
Title Uni Nottingham supply 21 germplasm lines to CIMMYT, Mexico 2018 
Description 21 lines sent to CIMMYT Mexico for phenotyping 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? No  
Impact none reported 
 
Title Uni Nottingham supply DH lines to USDA, Kansas State 
Description 5 new DH (Double Haploid) lines requested by USDA Kansas Sate, USA 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? No  
Impact none reported 
 
Title Uni Nottingham supply germplasm to CIMMYT Aug 2017 
Description Toolkit germplasm 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact none reported 
 
Title Uni Nottingham supply germplasm to IIWBR Oct 2017 
Description supply of germplasm 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact none reported 
 
Title Uni Nottingham supply germplasm to Joseph Breun 
Description germplasm requested by Joseph Breun 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact none reported 
 
Title Uni Nottingham supply germplasm to Malawi, Dec 2019 
Description Uni Nottingham supply 60 DH wheat/Amblyopyrum muticum lines and 35 DH wheat/Triticum urartu lines to Malawi 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title Uni Nottingham supply germplasm to Tunisia 
Description Uni Nottingham supply 61 DH wheat/Amblyopyrum muticum lines and 85 DH wheat/Triticum urartu lines to the Tunisia septoria screening platform 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title Uni Nottingham supply germplasm to USDA, Kansas Oct 2019 
Description Uni Nottingham supply 37 DH wheat/Amblyopyrum lines and 81 DH wheat/Triticum urartu lines to USDA, Kansas State 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact none reported 
 
Title Uni Nottingham supply germplasm to USDA, Kansas State Oct 2017 
Description Toolkit germplasm 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact non reported 
 
Description A meeting between CIMMYT and DFW funded by BMGF to discuss collaboration projects 
Organisation International Centre for Maize and Wheat Improvement (CIMMYT)
Country Mexico 
Sector Charity/Non Profit 
PI Contribution I organised a meeting funded by Bill and Melinda Gates Foundation brought together members of the BBSRC's coordinated wheat programme (Designing Future Wheat) with members of CIMMYT (who breed wheat for the resource poor in the developing world), discuss potential opportunities for interaction. These opportunities are taken forward by writing proposals for Newton , GCRF or IWYP funding calls
Collaborator Contribution See above
Impact This interaction is still ongoing between members of BBSRC's coordinated wheat programme (Designing Future Wheat) and researchers within CIMMYT with proposals being written for IWYP and Newton calls
Start Year 2018
 
Description Chairing wheat genetics symposium, Tuln, Austria 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Chaired a session of Wheat Genetic symposium
Year(s) Of Engagement Activity 2017
 
Description Conference organiser of PAG San Diego attracting 3800 plant and animal researchers 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I am on the organising committee of the largest plant and animal ag genomics conference attracting some 3800 researchers, policy makers, industry etc
Year(s) Of Engagement Activity Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019
 
Description DFW BREEDERS TOOLKIT ANNUAL MEETING 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact DFW Breeders Toolkit Annual Meeting - discussion and planning for Toolkit development and assessment, decisions on which to take forward; propose markers experiments; data handling;
Year(s) Of Engagement Activity 2018
 
Description DFW Presentation to DEFRA chief science advisor 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact A discussion of the outcomes and impact of WISP and DFW programmes
Year(s) Of Engagement Activity 2019
 
Description John Innes Centre open day to the public 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact To show the general public plant science research in practise.
Year(s) Of Engagement Activity 2017
 
Description Member of the board of the G20 wheat initative 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I am a board member of the G20 wheat initiative set up by the G20 agricultural ministers to facilitate coordination of wheat research in the G20 countries. We organise working group to facilitate such coordination, and to identify priorities for funding by funding agencies within the G20 countries
Year(s) Of Engagement Activity 2011,2012,2013,2014,2015,2016,2017,2018
 
Description Member of the management board of CGIAR wheat programme (CIMMYT and ICARD) to breed wheat for the resource-poor in the developing World 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A management board member of CGIAR wheat programme (CIMMYT and ICARD) to breed wheat for the resource-poor in the developing World
Year(s) Of Engagement Activity 2011,2012,2013,2014,2015,2016,2017,2018,2019
 
Description NIAB-TAG breeders day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Attendance at NIAB-TAG Breeders Day, which offers expert independent advice and research on variety choice, plant breeding, crop agronomy, plant protection, soils and cultivations with a mix of seminars, exhibits and field and glasshouse demonstrations
Year(s) Of Engagement Activity 2017
 
Description Norfolk forum Science festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact To provide the general public with an insight into plant science research
Year(s) Of Engagement Activity 2017
 
Description Organisation a day workshop at Eucarpia meeting , Clermont_Ferrand Paris, with INRA (French) and Proweizen (German ) and CIMMYT researchers 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The worship was to discuss possible collaborations which could lead to bids into the EU for funding
Year(s) Of Engagement Activity 2018
 
Description Presentation at the conference for the UK Millers and bakers (NABIM-london) on DFW 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Was asked to provide further information on the coordinated DFW programme
Year(s) Of Engagement Activity 2017
 
Description Presentation on DFW at the DEFRA stakeholders WGIN meeting at RRES 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Request for more information on the programme
Year(s) Of Engagement Activity 2017
 
Description Presentation on WISP/DFW to ACC1 Cereals and Grains conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact The presentation was to inform the cereal grain (rice, maize and wheat) processes community on the step change which has occurred in wheat research and the involvement of WISP/DFW in the step change
Year(s) Of Engagement Activity 2018
 
Description Presentation on WISP/DFW to the BBSRC legume community 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact The presentation was to describe how the UK coordinated wheat programme was put together, and the impact the programme has had on the step change which has occurred in wheat research
Year(s) Of Engagement Activity 2018
 
Description Presentation on WISP/DFW to the BBSRC rice community 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation described how the coordinated WISP/DFW UK wheat programme was put together, and the impact the programme has had on the step change which has occurred in wheat research
Year(s) Of Engagement Activity 2018
 
Description member of the G20 wheat Initiative Science board during 2019 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The members of the science board discuss how to better coordinate the wheat research activities in the G20 countries
Year(s) Of Engagement Activity 2019,2020
 
Description member of the management board of the CGIAR wheat programme (CIMMYT and ICARDA) to breed wheat for the resource poor in the developing world 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I offer advice to the management team of the wheat CGIAR programme
Year(s) Of Engagement Activity 2019,2020
 
Description member of the organising committee for PAG 2020 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Suggest plenary speakers for inviting to speak at PAG 2020 meeting attracting 3000 animal and plant researchers
Year(s) Of Engagement Activity 2019
 
Description participant in G20 wheat initiative Nitrogen use efficiency workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Participated in a discussion on priorities for funding in the wheat research area nitrogen use efficiency.
Year(s) Of Engagement Activity 2017