DFW - Designing Future Wheat - Work package 3 (WP3) - Germplasm
Lead Research Organisation:
Rothamsted Research
Department Name: Unlisted
Abstract
WP3 will accelerate the discovery and deployment of genes and alleles of high value for breeding, thus maximising impact from discoveries within WP1, WP2, and WISP and 20:20 Wheat. Tangible WP3 outputs for this purpose are referred to as 'toolkits', one composed of pre-breeding germplasm and the other the molecular markers needed to exploit that germplasm. WP3 addresses two topics. The first advances the genetic and genomic characterisation of foundation germplasm. This germplasm, together with the derived segregating populations and associated methodologies, will supply new toolkit genes, alleles, and markers. In the second the new genes, alleles and markers are validated and tested for their value in breeding. This gene discovery funnel produces packages of traits with molecular markers assembled into the two toolkits. WP3 channels new genes and alleles based on the discoveries of DFW and previous wheat programme outputs, into commercial wheat breeding. The breeders' toolkits and associated information will be improved and expanded, for the benefit of breeders and the wider plant community.
Publications
Alahmad S
(2018)
Crown rot of wheat in Australia: Fusarium pseudograminearum taxonomy, population biology and disease management
in Australasian Plant Pathology
Arora S
(2019)
Resistance gene cloning from a wild crop relative by sequence capture and association genetics.
in Nature biotechnology
Atkinson JA
(2020)
Soil strength influences wheat root interactions with soil macropores.
in Plant, cell & environment
Burridge AJ
(2017)
High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives.
in Methods in molecular biology (Clifton, N.J.)
Camargo AV
(2018)
Functional Mapping of Quantitative Trait Loci (QTLs) Associated With Plant Performance in a Wheat MAGIC Mapping Population.
in Frontiers in plant science
Clarke CK
(2017)
Quantifying rooting at depth in a wheat doubled haploid population with introgression from wild emmer.
in Annals of botany
Clifton-Brown JC
(2018)
Investigating the potential of novel non-woven fabrics for efficient pollination control in plant breeding.
in PloS one
Coulton A
(2020)
Segregation distortion: Utilizing simulated genotyping data to evaluate statistical methods.
in PloS one
| Description | During the project, we have provided BBSRC with a google.doc site, which lists all DFW outputs (including data access sites, germplasm generated, datasets generated, publications, and public engagement activities). This document is updated on a regular basis. All the objectives during the DFW reporting period have been met, and many exceeded. All the committees within DFW are running including the key data coordination task force (DCTF) and Breeder's toolkit committees, both with WP3 representation. • The DFW toolkit, including Breeders Toolkit field trialed for performance in multiple years. • Inclusion of ten new Rht-1 dwarfing alleles in the Breeder's Toolkit. • Optimized development of cost-effective phenotyping and phenotypic analysis • New targets for yield, flowering time and height identified and to be proposed for the breeders toolkit. Targets relating to NUE still under investigation. |
| Exploitation Route | further development of Breeders toolkits |
| Sectors | Agriculture, Food and Drink |
| URL | https://designingfuturewheat.org.uk/ |
| Description | further development of Breeders toolkits |
| First Year Of Impact | 2017 |
| Sector | Agriculture, Food and Drink,Digital/Communication/Information Technologies (including Software) |
| Impact Types | Societal,Economic |
| Title | Novel wheat germplasm for manipulation of crop height and response to stress |
| Description | Gene Pathway Role Alleles Current Generation Phenotype GA20ox1 GA GA biosynthesis A1b.B1b.D1c BC4F3 Semidwarf, 88% of WT height; field trial 2020 GA20ox2 GA GA biosynthesis A2b.B2b.D2b BC3F3 Semidwarf, 87% of WT height; field trial 2020 GA20ox4 GA GA biosynthesis A4b.B4b.D4c BC0F2 Semidwarf GA3ox2 GA GA biosynthesis A2b.B2b.D2b BC3F3 Extreme GA-deficient dwarf GA3ox3 GA GA biosynthesis A3b.B3c BC3F3 Low grain GA levels; no effect on seed development GA1ox1 GA GA modulation B1d BC5F3 High grain GA levels; no effect on seed development GA2ox3 GA GA inactivation A3b.B3b.D3b BC3F3 7% taller than WT Cadenza at BC3F3 GA2ox4 GA GA inactivation A1b.B1b.D1b BC3F1 t.b.d. GA2ox9 GA GA inactivation A9c.B9c.D9b BC3F1 ~10% taller than WT at BC0F2 EUI-1 GA GA inactivation A1b.B1c.D1c BC3F1 Elongated upper internodes ABA8OH1 ABA ABA inactivation A1c.B1b.D1b BC2F3 Evidence of increased seed dormancy D14 SL Strigolactone receptor A1b.B1b.D1b BC3F3 High tillering semi-dwarf D3 SL SL F-box B1c.D1d BC3F3 High tillering semi-dwarf D53 SL SL effector B1c (GOF) BC0F2 No phenotype observed SPL14 SL Shoot branching B1b (GOF) BC2F1 Low tillering, thicker culms SPL17 SL Shoot branching A1b (GOF) BC0F2 No phenotype observed TB1 SL Shoot branching A1b.B1b.D1b BC3F1 tbd IPI1 SL Shoot branching A1b.B1b.D1b BC0F2 No phenotype observed |
| Type Of Material | Biological samples |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | progress for Giberellin genes controlling plant and grain architecture |
| Title | NAIB supply germplasm to Hohenheim University Jul 2017 |
| Description | DFW germplasm seed request dispatched to Friedrich Longin, Hohenheim University |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to Agricultural Institute Osijek, Croatia |
| Description | NIAB supply 98 diverse lines to Dario Novoselovic (Agricultural Institute Osijek ) Croatia |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to Agroscope, July 2017 |
| Description | DFW germplasm seed request dispatched to Dario Fossati, Agroscope, Switzerland |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to Cerela Inc, Canada |
| Description | NIAB supply 98 diverse lines to Anna Archambault (Cerela inc.) Canada |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to Hohenheim Uni, Germany 2019 |
| Description | NIAB supply 98 diverse lines to Friedrich Longin ( Hohenhiem University) Germany |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to SENSAKO, S Africa |
| Description | germplasm Seed request dispatched to SENSAKO PTY in South Africa |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | none reported |
| Title | NIAB supply germplasm to SRUC Jul 2017 |
| Description | DFW germplasm seed dispatched to J R Adams, SRUC |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to Sejet Plant Breeding, Denmark Sept 2019 |
| Description | NIAB supply 98 diverse lines to Finn Borum ( Sejet Plant Breeding) Denmark |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to Uni Leicester Sept 2019 |
| Description | NIAB supply 98 diverse lines to James Higgins (University of Leicester ) UK |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | NIAB supply germplasm to Uni. Guelph Jul 2017 |
| Description | DFW germplasm seed requested by Ali Navabi, University of Guelph, Canada |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | Uni Nottingham supply 21 germplasm lines to CIMMYT, Mexico 2018 |
| Description | 21 lines sent to CIMMYT Mexico for phenotyping |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | none reported |
| Title | Uni Nottingham supply DH lines to USDA, Kansas State |
| Description | 5 new DH (Double Haploid) lines requested by USDA Kansas Sate, USA |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | none reported |
| Title | Uni Nottingham supply germplasm to CIMMYT Aug 2017 |
| Description | Toolkit germplasm |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | Uni Nottingham supply germplasm to IIWBR Oct 2017 |
| Description | supply of germplasm |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | Uni Nottingham supply germplasm to Joseph Breun |
| Description | germplasm requested by Joseph Breun |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | Uni Nottingham supply germplasm to Malawi, Dec 2019 |
| Description | Uni Nottingham supply 60 DH wheat/Amblyopyrum muticum lines and 35 DH wheat/Triticum urartu lines to Malawi |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | Uni Nottingham supply germplasm to Tunisia |
| Description | Uni Nottingham supply 61 DH wheat/Amblyopyrum muticum lines and 85 DH wheat/Triticum urartu lines to the Tunisia septoria screening platform |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | Uni Nottingham supply germplasm to USDA, Kansas Oct 2019 |
| Description | Uni Nottingham supply 37 DH wheat/Amblyopyrum lines and 81 DH wheat/Triticum urartu lines to USDA, Kansas State |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | none reported |
| Title | Uni Nottingham supply germplasm to USDA, Kansas State Oct 2017 |
| Description | Toolkit germplasm |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | non reported |
| Description | A meeting between CIMMYT and DFW funded by BMGF to discuss collaboration projects |
| Organisation | International Centre for Maize and Wheat Improvement (CIMMYT) |
| Country | Mexico |
| Sector | Charity/Non Profit |
| PI Contribution | I organised a meeting funded by Bill and Melinda Gates Foundation brought together members of the BBSRC's coordinated wheat programme (Designing Future Wheat) with members of CIMMYT (who breed wheat for the resource poor in the developing world), discuss potential opportunities for interaction. These opportunities are taken forward by writing proposals for Newton , GCRF or IWYP funding calls |
| Collaborator Contribution | See above |
| Impact | This interaction is still ongoing between members of BBSRC's coordinated wheat programme (Designing Future Wheat) and researchers within CIMMYT with proposals being written for IWYP and Newton calls |
| Start Year | 2018 |
| Description | Chairing wheat genetics symposium, Tuln, Austria |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chaired a session of Wheat Genetic symposium |
| Year(s) Of Engagement Activity | 2017 |
| Description | Conference organiser of PAG San Diego attracting 3800 plant and animal researchers |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I am on the organising committee of the largest plant and animal ag genomics conference attracting some 3800 researchers, policy makers, industry etc |
| Year(s) Of Engagement Activity | Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019 |
| Description | DFW BREEDERS TOOLKIT ANNUAL MEETING |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | DFW Breeders Toolkit Annual Meeting - discussion and planning for Toolkit development and assessment, decisions on which to take forward; propose markers experiments; data handling; |
| Year(s) Of Engagement Activity | 2018 |
| Description | DFW Presentation to DEFRA chief science advisor |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | A discussion of the outcomes and impact of WISP and DFW programmes |
| Year(s) Of Engagement Activity | 2019 |
| Description | John Innes Centre open day to the public |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | To show the general public plant science research in practise. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Member of the board of the G20 wheat initative |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I am a board member of the G20 wheat initiative set up by the G20 agricultural ministers to facilitate coordination of wheat research in the G20 countries. We organise working group to facilitate such coordination, and to identify priorities for funding by funding agencies within the G20 countries |
| Year(s) Of Engagement Activity | 2011,2012,2013,2014,2015,2016,2017,2018 |
| Description | Member of the management board of CGIAR wheat programme (CIMMYT and ICARD) to breed wheat for the resource-poor in the developing World |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A management board member of CGIAR wheat programme (CIMMYT and ICARD) to breed wheat for the resource-poor in the developing World |
| Year(s) Of Engagement Activity | 2011,2012,2013,2014,2015,2016,2017,2018,2019 |
| Description | NIAB-TAG breeders day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Attendance at NIAB-TAG Breeders Day, which offers expert independent advice and research on variety choice, plant breeding, crop agronomy, plant protection, soils and cultivations with a mix of seminars, exhibits and field and glasshouse demonstrations |
| Year(s) Of Engagement Activity | 2017 |
| Description | Norfolk forum Science festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | To provide the general public with an insight into plant science research |
| Year(s) Of Engagement Activity | 2017 |
| Description | Organisation a day workshop at Eucarpia meeting , Clermont_Ferrand Paris, with INRA (French) and Proweizen (German ) and CIMMYT researchers |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The worship was to discuss possible collaborations which could lead to bids into the EU for funding |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation at the conference for the UK Millers and bakers (NABIM-london) on DFW |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Was asked to provide further information on the coordinated DFW programme |
| Year(s) Of Engagement Activity | 2017 |
| Description | Presentation on DFW at the DEFRA stakeholders WGIN meeting at RRES |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Request for more information on the programme |
| Year(s) Of Engagement Activity | 2017 |
| Description | Presentation on WISP/DFW to ACC1 Cereals and Grains conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | The presentation was to inform the cereal grain (rice, maize and wheat) processes community on the step change which has occurred in wheat research and the involvement of WISP/DFW in the step change |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation on WISP/DFW to the BBSRC legume community |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | The presentation was to describe how the UK coordinated wheat programme was put together, and the impact the programme has had on the step change which has occurred in wheat research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation on WISP/DFW to the BBSRC rice community |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation described how the coordinated WISP/DFW UK wheat programme was put together, and the impact the programme has had on the step change which has occurred in wheat research |
| Year(s) Of Engagement Activity | 2018 |
| Description | member of the G20 wheat Initiative Science board during 2019 |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The members of the science board discuss how to better coordinate the wheat research activities in the G20 countries |
| Year(s) Of Engagement Activity | 2019,2020 |
| Description | member of the management board of the CGIAR wheat programme (CIMMYT and ICARDA) to breed wheat for the resource poor in the developing world |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I offer advice to the management team of the wheat CGIAR programme |
| Year(s) Of Engagement Activity | 2019,2020 |
| Description | member of the organising committee for PAG 2020 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Suggest plenary speakers for inviting to speak at PAG 2020 meeting attracting 3000 animal and plant researchers |
| Year(s) Of Engagement Activity | 2019 |
| Description | participant in G20 wheat initiative Nitrogen use efficiency workshop |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Participated in a discussion on priorities for funding in the wheat research area nitrogen use efficiency. |
| Year(s) Of Engagement Activity | 2017 |