Disease Pathogenesis

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

Comparative studies between differentially susceptible populations within a species and between closely related species are enormously powerful systems to interrogate the processes that lead to disease. Viral pathogenesis represents the sum of viral virulence factors and host susceptibility that lead to disease outcome. The suite of biological mechanisms involved during this process is complex and the outcome of infection can differ depending on variation in the virus, the host or through environmental influences. Consequently, studies using the natural viral host species offer the detailed information needed to advance our understanding of these complex processes.
Many of the viral pathogens we study cause differential disease within and between animal hosts. Susceptible species that do not show clinical signs can act as important virus reservoirs influencing viral evolution and transmission. Pirbright is uniquely placed with the capability to examine different host species responses to specific viral infections. These insights into the processes leading to disease are powerful tools to both identify future targets for disease control and for ensuring that any control strategies are not likely to exacerbate disease.
Pirbright is uniquely placed to examine the contribution of host genetics to differential immune responses. These complex traits are challenging to study, often requiring targeted and bespoke analysis in combination with more typical whole genome analyses. This has great potential to identify targets for gene editing to explore immune function in vitro, improve resilience and vaccine responses through informed breeding and potentially lead to developing transgenic animals.
Mathematical modelling of within-host dynamics has the potential to help understand mechanisms of viral pathogenesis and, in particular, factors influencing different clinical outcomes of infection. Moreover, it also provides a means to link pathogenesis with infectiousness and, hence, transmission between individuals.

Organisations

Publications

10 25 50
 
Description African swine fever virus (ASFV) causes an acute haemorrhagic fever in domestic pigs with high socio-economic impact. No vaccine is available limiting options for control. The role of the ASFV encoded CD2v protein in virus persistence is being studied. This protein causes virus and virus infected cells to bind to red blood cells. A predicted model of the ligand binding domain of CD2v protein was produced and the effect of amino acid substitutions on binding to red blood cells assayed. This identified a putative ligand binding site. We have also identified a number of African swine fever virus proteins that modulate the autophagy pathway that may play a role in disease pathogenesis.
We have developed many tools to study influenza immunity in pigs. Our data suggest that T cells can reduce severity of disease and pathology but not reliably decrease viral load. We have also demonstrated the utility of the pig influenza model in testing the protective efficacy of human therapeutic antibodies and investigating the role of Fc-FcR interactions in protective immunity.
Significant new knowledge was generated relating the induction of viral restriction in chickens to a diverse range of viruses. We have used the funding to examine the extent of genetic diversity in indigenous and rare breed birds in comparison to our inbred lines and commercial breeds of layers and broilers.
H9N2 avian influenza viruses cause mild to severe clinical disease in poultry. These viruses also infect humans and pigs. Our studies defined the mechanisms how non-structural proteins 1 (NS1) from different H9N2 virus strains impact on viral pathogenesis. We identified specific amino acid residues that involved in inhibition of host gene expression leading to inhibiting host cells protein "cleavage and polyadenylation specificity factor (CPSF30)" functions critical for host cell gene expression and induction of host antiviral innate immune responses.
We have developed immunological methods for the evaluation of effector and memory T cell responses in chickens, and demonstrated that two proteins of Marek's disease virus (MDV) are immunogenic antigens. The results demonstrate that oncogenic MDV and vaccine strain of the virus, CVI988-Rispens, activate both antigen specific effector and memory IFN-gamma producing T cells, and the magnitude of the effector T cell response is associated with resistant to the disease.
An initial model has been developed to describe the dynamics of foot-and-mouth disease virus in cattle and, specifically, changes over time in viral titres in different compartments (blood, nasal fluid and oropharyngeal fluid). This model has been linked with a simple transmission model to explore the relationship between viral titre in the host and the probability of transmission.
Using new reference genome assemblies from collaborators at the USDA-ARS we have been able to annotate highly variable regions containing immune gene from a range of livestock species and different breeds, including pigs, goats and cattle. This has enabled us to generate a predictive model of evolution as well as inform other researcher about the diversity they should expect when analysing immune responses to vaccination and infection.
Exploitation Route • Research outcomes enabling the policy makers to make inform decisions to implement risk-based control measures to reduce the impact of emerging avian influenza viruses on both animals and humans.
• More effective vaccines produced through this project are enabling reduction in virus circulation in environment thereby lessening both direct damage to poultry industry and the zoonotic transmission to humans.
• More accurate and rapid diagnostics produced through this project are enabling rapid decision making for implementation of optimal control measures in response to disease outbreak.
• Enhanced collaboration and co-production through this project is enhancing the science-based in the UK and the international collaborating institutions in the shape of research capacity and skills that are facilitating medium to long-term science-based gains in productivity in livestock and poultry farming systems
• The methods could be used to analyse T cell responses to vaccines against other poultry pathogens, and examine host-pathogen interaction against other viral infections in chickens.
• Provide vital information to the UK agriculture sector in preparing for a potential incursion of BTV in 2019. Study outcomes will enable evidence based decisions of policy makers, veterinarians and farmers.
Sectors Agriculture, Food and Drink

 
Description Information obtained using a bovine challenge model RSV is being used to inform human challenge models. As part of the Human Infection Challenge Network, our work underpins the development of policies to regulate, enhance and support human challenge models in the UK and EU. In coordination with regulatory agencies and funding bodies, our work is being used to determine new regulatory frameworks for human challenge studies. The data generated by this research has allowed additional funding from other sources including EU and UKRI responsive mode. In addition, the genomic and functional data for the avian immune genes has provided fundamental data for the analysis these genes in additional species. This should enhance the quality of life and health of livestock and those involved in livestock farming. The research identified factors that aiding virulence and persistence of avian influenza viruses in different animals and bird species. The outcome of this research directly feed into surveillance and risk assessment of emerging and re-emerging viruses, enabling implementation of rapid and appropriate disease mitigation strategies.
Sector Agriculture, Food and Drink
 
Description Advisor on International Atomic Energy Association Collaborative Reserach Project on African swine fever
Geographic Reach Africa 
Policy Influence Type Influenced training of practitioners or researchers
Impact Provided training to participants from Veterinary Services and Institutions in Developing Countries in Africa on African swine fever
 
Description Contributing to the chapter on Marek's disease for the OIE Terrestrial Manual
Geographic Reach Multiple continents/international 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
Impact The contribution to the Terrestrial Manual on the recent advances in the diagnosis and control of Marek's disease will impact on improved and better control of the disease to reduce losses and improved food security
URL http://www.oie.int/
 
Description Contribution to Annual Report of the Chief Medical Officer UK (2017), Genomics Chapter 9.
Geographic Reach Europe 
Policy Influence Type Gave evidence to a government review
 
Description Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals'
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Development and Evaluation of PPR VIVA vaccines
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in other policy documents
Impact Peste des petits ruminants (PPR) is a highly contagious disease, severely affects small ruminants in almost 70 countries in Africa, the Middle East and parts of Asia. It causes USD 1.5 to 2 billion in losses each year in regions that are home to over 80% of the world's sheep and goats and to more than 330 million of the world's poorest people, many of whom depend on them for their livelihoods. The estimated current expenditure on PPR vaccination ranges between USD 270 and 380 million. The annual impact of PPR alone may be valued at between USD 1.45 and 2.1 billion per year. Approximately a third of the global financial burden of PPR is borne by Africa, with a further quarter borne by South Asia. This burden will be removed with the successful eradication of PPR. The undiscounted costs for a fifteen-year Global control Strategy of FAO and OIE are between USD 7.6 and 9.1 billion, with the first five years costing between USD 2.5 and 3.1 billion. (http://www.fao.org/3/a-i4460e.pdf). PPR is currently controlled by vaccination using mainly two live attenuated PPRV vaccines (Nigeria 75/1 and Sungri 96). However, the current vaccines and serological tests are unable to enable Differentiation between naturally Infected and Vaccinated Animals (DIVA). This factor precludes meaningful assessment of vaccine coverage and epidemiological surveillance based on serology, in turn reducing the efficiency of control programmes. Therefore, it is almost impossible to assess the quality and efficacy of existing PPR vaccines without knowing whether positive animals are vaccinated or naturally infected. Unlike rinderpest, where cattle and buffalo were primary hosts, in PPR, new crops (about 30-40%) of lambs and kids are produced every year and are the most susceptible population to bring back new PPR outbreaks6&7. Therefore, it is likely that the cycle of vaccinations and PPR outbreaks will continue until and unless we reach the stage of 80-90% herd immunity. Therefore, development of a marker vaccine and associated DIVA diagnostics will enable the assessment of vaccine efficacy which is essential for any successful vaccination campaign. https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-peste-des-petits-ruminant The availability of a recombinant PPRV vaccine with a proven functionality is a prerequisite for the development of novel vaccines that may enable the development of DIVA tools for PPRV diagnostics. In the DBT-BBSRC FADH BB/L004801/1 grant we have rescued Sungri 96 and Nigeria 75/1 vaccine strains. Both the vaccine strains were rescued from respective synthetic c-DNA clones with mutations in the variable part of C terminus of the nucleocapsid (N) gene similar to Dolphin Morbillivirus (DMV) to enable negative marker DIVA vaccines. These two DIVA vaccines along with parental vaccines have been recently tested in a pilot studies in goats. Both the DIVA and parent vaccines provided safety, stability and protection for vaccinated goats whereas the control animals were clinically infected. Patent applications have been made to protect these DIVA vaccines. Agreements are being done with vaccine industries for the licensing and commercialisation.
URL https://www.pirbright.ac.uk/press-releases/2018/09/pirbright-collaboration-provides-tools-peste-des-...
 
Description EFSA working group on lumpy skin disease
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
Impact Advice to European Commission and affected countries about control and surveillance for lumpy skin disease
URL https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2018.5452
 
Description Expert Reviewer for Veterinary Vaccines, European Medicines Agency
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
 
Description Human Infection Challenge Models for Vaccination
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Member of the British Society for Immunology Forum
Geographic Reach National 
Policy Influence Type Influenced training of practitioners or researchers
URL https://www.immunology.org/
 
Description Organised seminar series on Sequencing, Virus Evolution and Molecular Biology at Pirbright (with Luca Ferretti and Graham Freimanis)
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Participation in the Management of the Oxford DTP
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact As part of the Management committee of the Oxford-Pirbright-Oxford Brookes-Diamond doctorate Training Partnership, contributed to the success of the DTP in meeting the objectives, recruitment and review
URL http://www.biodtp.ox.ac.uk/
 
Description Taught course on introductory bioinformatics to PhD students at Pirbright
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Training MSc students
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
Impact Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.
 
Description BBSRC - responsive mode
Amount £187,370 (GBP)
Funding ID BB/S007911/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2019 
End 03/2022
 
Description BBSRC Industrial CASE (iCASE)
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2019 
End 09/2022
 
Description BBSRC Newton Fund Swine and Poultry Research Initiative
Amount £865,623 (GBP)
Funding ID BB/R01275X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 06/2021
 
Description BBSRC-iCASE
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2019 
End 09/2023
 
Description Bilateral BBSRC-FAPESP Research Grant: Dissecting Essential Roles of Chicken Interferon Stimulated Genes in the Pathobiology of Poultry Viruses
Amount £383,644 (GBP)
Funding ID BB/M008681/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2015 
End 08/2018
 
Description British Egg Marketing Board PhD studentship
Amount £76,000 (GBP)
Organisation British Egg Marketing Board 
Sector Private
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description Dissecting the molecular pathways of MDV oncoprotein Meq for understanding pathogenesis and aid vaccine development
Amount £440,000 (GBP)
Funding ID BB/R007896/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description FLI- TPI joint PhD studentships Avian influenza immunology markers.
Amount £100,000 (GBP)
Organisation Friedrich Loeffler Institute 
Sector Public
Country Germany
Start 07/2018 
End 06/2022
 
Description Further development of infectious bronchitis virus vaccines adapted for production in cell culture
Amount £262,701 (GBP)
Funding ID BB/R019576/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 03/2020
 
Description Japan Partnering Award
Amount £49,500 (GBP)
Funding ID BB/P025803/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 08/2021
 
Description LVIF
Amount $1,500,000 (CAD)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 04/2018 
End 09/2020
 
Description NC3Rs Research Project Grant
Amount £436,339 (GBP)
Funding ID NC/R001138/1 
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Private
Country United Kingdom
Start 12/2017 
End 11/2019
 
Description Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Amount £841,995 (GBP)
Funding ID BB/R012679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2020
 
Description Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative. Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry.
Amount £497,995 (GBP)
Funding ID BB/R012679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2020
 
Description Pathfinder BB/R012431/1 Grant holder: Dr Mark Fife. Grant title: chIFITM knockdown/knockout technology as a platform technology for increased vaccine yields in SPF Eggs.
Amount £12,500 (GBP)
Funding ID BB/R012431/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2017 
End 02/2018
 
Description Reducing the Economic and Zoonotic Impact of Avian Influenza (REZIAI): delivering novel vaccines and diagnostics from laboratory to the field.
Amount £400,000 (GBP)
Funding ID BB/S013792/1 
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 02/2019 
End 01/2021
 
Description Responsive mode: Avian influenza H7N9 virus evolution: defining the impact of internal genes on virus infection in avian and mammalian species
Amount £668,314 (GBP)
Funding ID BB/N002571/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2016 
End 06/2019
 
Description Responsive mode: Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections.
Amount £535,337 (GBP)
Funding ID BB/R007292/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2018 
End 01/2021
 
Description SFS-10-2017 - Research and approaches for emerging diseases and pests in plants and terrestrial livestock: Addressing the dual emerging threats of African Swine Fever and Lumpy Skin Disease in Europe (DEFEND)
Amount € 5,986,250 (EUR)
Funding ID 773701 
Organisation European Commission H2020 
Sector Public
Country Belgium
Start 06/2018 
End 05/2023
 
Description Seed Catalyst Award
Amount £25,527 (GBP)
Funding ID ISCF-TFPSA-Pirbright 
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 11/2018 
End 02/2019
 
Description Taiwan Partnering Award
Amount £40,700 (GBP)
Funding ID BB/S020624/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start  
 
Description The Global Challenges Research Fund
Amount £640,473 (GBP)
Organisation Research Councils UK (RCUK) 
Sector Public
Country United Kingdom
Start 04/2019 
End 03/2024
 
Description UK-China partnership on Global Food Security: Combating avian tumor diseases for sustainable poultry proudction
Amount £382,000 (GBP)
Funding ID BB/R007632/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2020
 
Description ZELS-AS: Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan.
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2015 
End 09/2019
 
Title An ex vivo IBDV infection model using a chicken primary B cell culture system 
Description Recently, it has become possible to culture chicken primary B cells in vitro in the presence of a soluble construct of chicken CD40L that was made at The Pirbright Institute. We have demonstrated that these chicken primary B cells can be infected with infectious bursal disease virus (IBDV). This system will be validated and expanded to other B cell tropic viruses in order to replace the use of infected chickens in research, in an NC3Rs funded grant. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2017 
Provided To Others? Yes  
Impact Using this system, we have determined that chicken primary B cells respond to IBDV infection by inducing Type I IFN responses. However, the induction is more pronounced in cells infected with an attenuated strain compared to a very virulent strain. This is consistent with the very virulent strain down-regulating antiviral responses to a greater extent than other strains which may, in part, explain its enhanced virulence. We also observed a reduction in the expression of key genes involved in B cell proliferation and activation following IBDV infection which was only possible by using the primary B cell culture system. 
URL https://www.ncbi.nlm.nih.gov/pubmed/?term=broadbent+dulwich
 
Title Cattle MHC genotyping 
Description Using the sequence data generated through the targeted pull down of MHC, we developed a full gene and more targeted PCR approach to genotype cattle for the MHC class I region. This has been applied to many hundreds of samples to enable us to select individuals for breeding as well as survey genetic diversity in beef and diary herds. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact After publication which we anticipate in 2019, we will appy this method to targeted herds and are already attracting industry interest. 
 
Title Comprehensive analysis of genetic variants across immune loci in indigenous breeds. 
Description The interferon-induced transmembrane (IFITM) protein family comprises a class of restriction factors widely characterised in humans for their potent antiviral activity. Their biological activity is well documented in several animal species, but their genetic variation and biological mechanism is less well understood, particularly in avian species. Here we report the complete sequence of the domestic chicken Gallus gallus IFITM locus from a wide variety of chicken breeds to examine the detailed pattern of genetic variation of the locus on chromosome 5, including the flanking genes ATHL1 and B4GALNT4. We have generated chIFITM sequences from commercial breeds (supermarket-derived chicken breasts), ancient bone samples, indigenous chickens from Nigeria (Nsukka) and Ethiopia, European breeds and inbred chicken lines from The Pirbright Institute, totalling of 211 chickens. Through mapping of genetic variants to the latest chIFITM consensus sequence our data reveal that the chIFITM locus does not show structural variation in the locus across the populations analysed, despite spanning diverse breeds from different geographic locations. However, single nucleotide variants (SNVs) in functionally important regions of the proteins within certain groups of chickens were detected, in particular the European breeds and indigenous birds from Ethiopia and Nigeria. In addition, we also found that two out of four SNVs located in the chIFITM1 (Ser36 and Arg77) and chIFITM3 (Val103) proteins were simultaneously under positive selection. Together these data suggest that IFITM genetic variation may contribute to the capacities of different chicken populations to resist viral infection. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2019 
Provided To Others? Yes  
Impact This paper is currently in press in. BMC Genomics. 
 
Title De 
Description // 
Type Of Material Model of mechanisms or symptoms - human 
Year Produced 2017 
Provided To Others? No  
Impact // 
 
Title Detection of HA antigens of avian influenza viruses 
Description Assay for detection of recombinant HVT expressing HA antigens of avian influenza viruses 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact This new method will help to detect recombinant HVT vaccine expressing HA antigen in infected cell. 
 
Title Development of tetramers to study immune responses in pigs 
Description The pig is a natural and important host of influenza viruses and is physiologically more comparable to humans than other animal models in terms of size, respiratory tract biology and volume. It is also an important vector in the birds to human infection cycle. A major drawback of the current pig model is the inability to analyze antigen-specific CD8+ T-cell responses, which are critical to respiratory immunity. We addressed this knowledge gap using an established in-bred pig model with a high degree of genetic identity between individuals, including the MHC (Swine Leukocyte Antigen (SLA)) locus. We developed a toolset that included long-term in vitro pig T-cell culture and cloning and identification of novel immunodominant influenza-derived T-cell epitopes. We also generated structures of the two SLA class I molecules found in these animals presenting the immunodominant epitopes. These structures allowed definition of the primary anchor points for epitopes in the SLA binding groove and established SLA binding motifs that were used to successfully predict other influenza-derived peptide sequences capable of stimulating T-cells. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact Peptide-SLA tetramers were constructed and used to track influenza-specific T-cells ex vivo in blood, the lungs and draining lymph nodes. Aerosol immunization with attenuated single cycle influenza viruses (S-FLU) induced large numbers of CD8+ T-cells specific for conserved NP peptides in the respiratory tract. Collectively, these data substantially increase the utility of pigs as an effective model for studying protective local cellular immunity against respiratory pathogens. 
 
Title IBDV Reverse Genetics System 
Description We have developed a reverse genetics system for IBDV. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity. 
 
Title Optimisation of chicken kidney cell culture preparation 
Description We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus. 
Type Of Material Cell line 
Year Produced 2017 
Provided To Others? No  
Impact This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information. 
 
Title Optimisation of preparation of TOCs from adult chickens 
Description Tracheal organ cultures (TOCs) are prepared from chicken embryos and used to assess growth of avian viruses that are not able to be propagated in cell culture. We have optimised the method of TOCs preparation from 3-week old chickens and are using these to assess viral genetic stability and phenotype in organ cultures that are a similar environment to the site of replication in whole chickens. 
Type Of Material Cell line 
Year Produced 2018 
Provided To Others? No  
Impact We hope that this technique will reduce the number of chickens required for in vivo experiments, in line with the 3Rs, and plan to publish the method in due course. 
 
Title Stable chicken cell lines overexpressing chicken IFITM1,2 and 3 
Description In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV. 
 
Title chimeric IBDVs 
Description Andrew Broadbent has engineered chimeric IBDVs expressing individual genes from a very virulent strain in the backbone of an attenuated strain. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact These chimeric strains can be used to define viral determinants of virulence. 
 
Title method for identification of lung Tissue resident memory cells in pigs 
Description Recent overwhelming evidence indicates the importance of local tissue-resident memory T cells (TRM) in protective immunity. Most work on TRM has been performed in mice and the TRM defined as inaccessible to intravenously administered anti-T cell antibody. However there are very few data on TRM in large animals. For the first time we have defined TRM in the pig influenza model. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact The identification of TRM and their robust protective capacity in site-specific infection has provided a new paradigm by which to assess T cell-mediated responses and an important new target for vaccine design. Since swine are an economically important species, are used as a large animal model for human infection and play a key role in the emergence of novel and potentially zoonotic influenza viruses, the identification of TRM in pigs will allow us to study their role in immunity to swine influenza.and how best to induce them by immunisation. 
 
Title tetracysteine-tagged IBDV 
Description Andrew Broadbent has engineered a tetracysteine (TC)-tagged IBDV that can be used in live cell imaging and co-infection studies with the split-GFP IBDV. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact This tool, combined with the split-GFP IBDV, will enable us to study co-infection, super-infection exclusion, and the intracellular requirements for viral reassortment. 
 
Title Genome browser for MDV infected chicken cells 
Description The database integrates and makes available the results of the bioinformatics analysis of several experiments involving high-throughput sequencing of chicken cells infected by several viruses (mainly Marek's Disease Virus) generated within the Avian Viral Disease group at the Pirbright Institute. 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? Yes  
Impact The genome browser allows the researchers to meaningfully mine and integrate the large amount of data produced by their experiments, guiding subsequent experimental hypothesis-driven validation in the lab. 
 
Title Genome browser for mosquitos at different developmental stages 
Description The database integrates and makes available the results of the bioinformatics analysis of several experiments involving high-throughput sequencing of mosquito cells at different developmental stages generated within the Vector-borne Viral Disease group at the Pirbright Institute. 
Type Of Material Database/Collection of data 
Year Produced 2016 
Provided To Others? Yes  
Impact The genome browser allows the researchers to meaningfully mine and integrate the large amount of data produced by their experiments, guiding subsequent experimental hypothesis-driven validation in the lab. 
 
Title Method to detect the expression of repetitive genes 
Description Reconstructing expression levels of genes having a high repeat content is difficult, due to the impossibility of assigning RNA-sequencing that have multiple mappings to their correct originating location. In collaboration with the Immunogenetics group, the Integrative Biology and Bioinformatics groups are developing a data analysis method to circumvent the problem.The method has already been shown to be effective on some KIR genes in cattle. An essential component of the immune system, their family is rich in repeated members and repetitive regions, making the technique essential if one wants to study them. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact Being able to resolve the expression of repetitive genes, which are plentiful in most mammalian genomes, would be essential to the high-precision study of many important gene families, including most of the genes of the immune system. The technique has already proven itself effective on a real-life problem, and holds the potential to be extended to many more biological systems 
 
Title South-African buffalo FMDV sequences 
Description We have sequenced FMDV buffalo samples originated from a EEID project entitled: Persistence of a Highly Contagious Pathogen: Ecological and Evolutionary Mechanisms in Foot-And-Mouth Disease Virus. This project aims to understand why a highly contagious pathogen such as FMDV, which induces a rapid host immunity and depletes the supply of susceptible host, is able to persist in isolated buffalo populations and thus avoid auto-extinction. The centre piece of the project is a cohort study which involves an established FMDV-positive breeding herd of ca. 70 buffalo in a 900-hectare enclosure surrounded by double game fencing housing buffalo in isolation from other herds in the Kruger National Park (South Africa). The entire herd is being monitored for three years (animals are sampled every 2 months (serum, tonsil swabs, probang) to trace FMDV transmission events, allowing us to define FMD infection dynamics across the susceptible calf cohorts and amongst adults. So far, FMDV genomes from 101 samples have been deep sequenced by illumina. Samples from the last year captures are currently being analysed and virus is currently being isolated. The second experiment is an experimental study, which involves a group of naïve buffalo experimentally infected with either a SAT-1, SAT-2 or SAT-3 virus. The infected buffalo were then allowed to mingle with susceptible animals and transmission of FMDV to naïve animals was monitored during the acute infection but also from persistently infected animals. So far, FMDV genomes from 48 samples obtained at days 2, 30 and 160 of the experiment have been deep sequenced. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? No  
Impact The samples sequenced come from South Africa, which is an LMIC, While FMDV infection in cattle is very well studied, the mechanisms of persistence in buffalo, which is thought to be the natural reservoir of the infection, are not well understood. Such understanding would be essential to inform better policies to understand and manage FMDV, which is an economically important scourge of cloven hooved animals in LMICs. All the sequences will be made available on the Transboundary Portal which is being developed at Pirbright. 
 
Title Viral assembly pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust viral assemblies out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, assembling of viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; uneven coverage due to low-quality material or the genomic material of the virus being RNA; the viral nucleic acid being almost lost in the background of nucleic acid of the host. Our pipeline overcomes most of those problems, and is even able to detect different viral strains being present together in the same sample. It can also be used on metagenomic environmental samples. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Title Viral variant calling pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust variant calling for viral sequences out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, calling variant for viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; very high genome coverage, which slows down most existing variant callers; the presence of a potentially very high number of different haplotypes for RNA viruses, which is an unusual scenario in variant calling. Our fast and sensitive Bayesian pipeline overcomes most of those problems. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Title Zimbabwe FMDV sequences 
Description Foot-and-mouth disease virus (FMDV) causes an acute vesicular disease in domestic cloven-hooved animals. However, in the African buffalo (Syncerus caffer) clinical disease is rarely observed and following infection virus is persistently carried in the oesophageal-pharyngeal area of the upper respiratory tract. During the 1990s oesophageal-pharyngeal scrapings were collected from free-living African buffalo in multiple herds in six different geographic areas of Zimbabwe. We sequenced over 140 FMD viruses each belonging to one of the Southern African Territories (SAT) serotypes (SAT 1, SAT 2 and SAT 3) from primary bovine thyroid cells. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? No  
Impact This dataset has been generated from samples collected in Zimbabwe, which is a low-income country. Those viral sequences will be essential to help elucidating the nature of persistent FMDV infection in African buffalo, which is supposed to be the main virus reservoir in vivo. As the samples track viral infection and evolution over several years and across a number of different herds in different national parks and conservatories, the dataset will also inform better animal management and conservation. A better understanding of FMDV persistence would also be essential to mitigate the economic burden generated by the disease, which is a scourge of cloven-hoofed animals in LMICs. All the sequences will be made available through the Transboundary Pathogen portal that is being developed at Pirbright. 
 
Description Aerosol delivery of vaccines and therapeutics 
Organisation Aerogen
Country Ireland 
Sector Private 
PI Contribution Developed the pig influenza model which is a natural host pathogen system
Collaborator Contribution Provided expertise and equipment for aerosol delivery
Impact Successfully delivered vaccines by aerosol
Start Year 2015
 
Description Anihwa Call 1 
Organisation Friedrich Loeffler Institute
Country Germany 
Sector Public 
PI Contribution 1. Conducted challenge experiments in goats to study pathogenicity. 2. Developed NGS technology to sequence PPR whole genome.
Collaborator Contribution FLI has conducted transmission study between different species ( Goats, pig, camel). For the first time they showed that pigs are clinically infected by PPR virus.
Impact Joint Publications
Start Year 2013
 
Description Avian influenza H7N9 virus evolution: defining the impact of internal genes on virus infection in avian and mammalian species 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution Provision of samples and reagents for research
Collaborator Contribution Provision of samples, reagents for research and research facilities
Impact Research output entitled "A restriction digestion and ligation independent technique for cloning influenza gene segments into pHW2000" was presented at January 17th to 19th 2018. Fortuna Hotel, Lang Ha Street, Hanoi, VietNam (Presented by postdoctoral scientist Dr Sushant Bhat
Start Year 2016
 
Description Avian influenza H7N9 virus evolution: defining the impact of internal genes on virus infection in avian and mammalian species 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution (1) Exchange of research methods and reagents
Collaborator Contribution (1) Exchange of research methods and reagents. (2) Provision of training to PhD students and postdoctoral Scientist (3) Critical review of experimental data and manuscripts.
Impact (1) Joint funding: BBSRC Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative (2018-2021). Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry. Value: £497,995.93. UK Co-Is: Prof John McCauley (Crick Institute), Dr Richard Reeve (University of Glasgow). Collaborators from China: Dr Yipeng Sun, Prof Jinhua Liu and Dr Juan Pu (China Agricultural University), Prof Yongqing Li (Beijing Academy of Agriculture and forestry Sciences), Dr Wenfei Zhu (National Institute for Viral Disease Control and Prevention, China CDC).
Start Year 2007
 
Description Bridget Penman 
Organisation University of Warwick
Department School of Life Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution We are providing genetic data and diversity measures of MHC and associated receptors to inform the modelling efforts to elucidate which selection pressures are the main drivers for the types of diversity we see in extant species.
Collaborator Contribution They are experts in mathmatical modelling and will provide data to help explain genetic diversity in cattle.
Impact Not yet
Start Year 2017
 
Description Challenge of transgenic chickens with influenza viruses 
Organisation Imperial College London
Department Section of Virology
Country United Kingdom 
Sector Academic/University 
PI Contribution We have challenged transgenic chickens with recombinant avian influenza viruses to determine outcome; infectivity, transmission, pathogenicity, dissemination within host. We also processed the samples generated from these animal experiments at The Pirbright Institute.
Collaborator Contribution Imperial College (Wendy Barclay and Mike Skinner) generated recombinant avian influenza viruses for challenge.
Impact This collaboration has resulted in the successful award of a joint grant with imperial college aiming to carry out challenge experiments on novel transgenic chickens to facilitate control of avian influenza virus in the poultry population. BB/S007911/1 - Investigating the role of ANP32A in the replication of avian Influenza virus
Start Year 2018
 
Description Collaboration studentship BTV 
Organisation University of Surrey
Department Faculty of Health and Medical Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaborative studentship with virologist at the University of Surrey, student registered at the University
Collaborator Contribution Intellectual input, training of student
Impact Thesis http://epubs.surrey.ac.uk/845643/
Start Year 2013
 
Description Development of novel diagnostic tools and vaccines 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation Imperial College London
Department School of Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation National Agricultural Research Centre
Country Pakistan 
Sector Public 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation University of Oxford
Department Oxford University Clinical Research Unit Vietnam (OUCRU)
Country Viet Nam 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation University of Veterinary & Animal Sciences
Country Pakistan 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of tetramers in the Babraham pig model 
Organisation Cardiff University
Country United Kingdom 
Sector Academic/University 
PI Contribution Developed the aerosol delivery method of vaccine and provided material (spleen, BAL and blood) from inbred animals (Babraham pigs) following immunisation with the candidate universal vaccine, S-FLU.
Collaborator Contribution Developed porcine influenza specific class I tetramers and provided the epitope map of Babrahams S-Flu responses to NP, M1, M2, PB1 and PB2 that could be presented by SLA-1 or SLA-2 Grew pig T cell clones for the first time in 30 years.
Impact Multidisciplinary - immunology, virology, protein chemistry. Paper not yet published
Start Year 2014
 
Description Efficacy of a candidate universal influenza vaccine, S-FLU. 
Organisation University of Oxford
Department Radcliffe Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Tested the immunogenicity and efficacy of a candidate universal influenza vaccine, S-FLU, in pigs. For the first time we demonstrated that S-FLU when administered by aerosol can reduce viral load in nasal swabs and lung in pigs after influenza virus challenge. We have shown that the most efficient way to administer this vaccine is by aerosol.
Collaborator Contribution Professor Alain Townsend has developed the S-FLU vaccine.
Impact The most efficient way to induce immune response in the lung is after aerosol delivery of LAIV vaccines in pigs. The most efficient way to induce cross-protective immunity is by aerosol delivery of S-FLU to the lungs of pigs
Start Year 2014
 
Description Horizon Discovery Ltd Cambridge Research Park, United Kingdom 
Organisation Horizon Discovery Group plc
PI Contribution Verify IFITM knock-out cell by qPCR and immuno-blotting. Verification of further knock-out cell lines by DNA sequencing, immuno-blotting and qPCR Cell infection with Avian Influenza Viruses Assess the viral titre of cells infected with Influenza Viruses in K/O and wildtype cells. Quantitative analysis of viral infection in IFITM K/O cells Demonstrate increased AIV viral titres at lab scale and estimation of what this may equate to with respect to vaccine dose yields. Analysis of IFITM K/O cell lines permissivity to a range of additional animal viruses (Relevant to the LVIF). Milestone 7: Demonstrate permissivity increased and viral titres for a range of LVIF relevant viruses in the edited cell lines.
Collaborator Contribution Develop a CRISPR/cas9 gene editing system to specifically target the chIFITM locus in avian cell culture
Impact Grant funding
Start Year 2017
 
Description ILRI Research Collaboration (Kenya, March 2017) 
Organisation International Livestock Research Institute (ILRI)
Country Kenya 
Sector Charity/Non Profit 
PI Contribution Exchange of ideas and samples for indigenous breeds of livestock
Collaborator Contribution Contributed samples for analysis.
Impact Analysis of indigenous breed genomic sequences for Immune function genes.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of genetic markers in the haemagglutinin glycoprotein critical for antigenic activity of H9N2 avian influenza viruses. 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution d provision of reagents and virus samples.
Collaborator Contribution Technical training to PhD students and use of research facilities
Impact (1) Talk: Avian influenza virus evolution: immune escape, increase in zoonotic potential and fitness in poultry. Presented at "Prevention and Control Techniques for Infectious Diseases in Livestock and Poultry", 27th 29th August 2017, Beijing, China. (2) Impact of avian influenza virus evolution on antigenicity and zoonotic infection potential. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan on 23rd August 2017. (3). Molecular determinants of H9N2 avian influenza virus influencing antigenicity and receptor binding. Presented at CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. 31st August - 1st September 2017 at the Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. (4) Overview of ongoing research on Poultry diseases in United Kingdom. Presented as at International poultry Stakeholders work on poultry biosecurity in Sri Lanka from 26th February to 3rd March 2017. (5). Evolutionary fitness of avian influenza viruses and development of improved vaccines and diagnostics. Presented as invited seminar speaker at RVC (Hawkshead Campus), UK. 19th October 2016.
Start Year 2012
 
Description Identification of genetic markers in the haemagglutinin glycoprotein critical for antigenic activity of H9N2 avian influenza viruses. 
Organisation Imperial College London
Department Section of Virology
Country United Kingdom 
Sector Academic/University 
PI Contribution d provision of reagents and virus samples.
Collaborator Contribution Technical training to PhD students and use of research facilities
Impact (1) Talk: Avian influenza virus evolution: immune escape, increase in zoonotic potential and fitness in poultry. Presented at "Prevention and Control Techniques for Infectious Diseases in Livestock and Poultry", 27th 29th August 2017, Beijing, China. (2) Impact of avian influenza virus evolution on antigenicity and zoonotic infection potential. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan on 23rd August 2017. (3). Molecular determinants of H9N2 avian influenza virus influencing antigenicity and receptor binding. Presented at CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. 31st August - 1st September 2017 at the Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. (4) Overview of ongoing research on Poultry diseases in United Kingdom. Presented as at International poultry Stakeholders work on poultry biosecurity in Sri Lanka from 26th February to 3rd March 2017. (5). Evolutionary fitness of avian influenza viruses and development of improved vaccines and diagnostics. Presented as invited seminar speaker at RVC (Hawkshead Campus), UK. 19th October 2016.
Start Year 2012
 
Description Improving vaccines and diagnostics for avian influenza viruses 
Organisation Zagazig University
Country Egypt 
Sector Academic/University 
PI Contribution Provision of Research training, analysis of field samples
Collaborator Contribution Provision of Field data and samples
Impact Characterisation of Avian Influenza Virus Hemagglutinin from Egyptian Chicken Flocks during 2016 Outbreaks. Presented at Global Alliance for Research on Avian Diseases conference. Hanoi, VietNam17-19 January 2018.
Start Year 2017
 
Description Industrial partnership-Aviagen 2018 
Organisation Aviagen Group
Country United States 
Sector Private 
PI Contribution We have established a significant industrial partnership with Aviagen group Ltd. The nature of this work remains confidential.
Collaborator Contribution The nature of this work remains confidential.
Impact The nature of this work remains confidential.
Start Year 2018
 
Description Interferon stimulated genes 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Hosted meetings and carried out preliminary experiments
Collaborator Contribution Contributed reagents and expertise
Impact Preliminary data was used to support an MSc project. This has since led to a publication and a PhD project.
Start Year 2016
 
Description Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses 
Organisation Royal Veterinary College (RVC)
Department Veterinary Basic Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution The overarching aims of the proposed research are to improve controls against H5 subtype of avian influenza viruses infecting poultry by investigating underlying mechanisms that define how vaccines renders effectiveness and develop novel approaches enhancing the effectiveness of H5 avian influenza vaccines. We recently generated a panel of monoclonal antibodies (mAbs) that recognise a variety of different clades of H5 viruses. Propagation of AIV in eggs or cultured cells together with mAbs drives virus to escape from mAbs neutralisation activity. These classical virus neutralization assays (VN) followed by HA gene sequence analysis allowed us to defined the role of amino acid substitutions that are associated with evasion of antibody neutralization through emergence of antigenic variants and failure of vaccine efficacy.
Collaborator Contribution provided intellectual input for achieving prescribed objectives of this work.
Impact Talk entitled "Molecular determinants for antigenicity and vaccine efficacy of avian influenza viruses" was presented at Newton Agham Researcher Links Workshop " Novel Vaccines and Diagnostic Technologies against Emerging and re-emerging Veterinary Pathogens" at Rizal Park Hotel, Manila, Philippines, 4-7 February 2019.
Start Year 2019
 
Description Investigating the role of the SH protein of RSV in the duration of immunity 
Organisation Imperial College London
Department Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution We provided initial data supporting the hypothesis that the small hydrophobic protein of bovine respiratory syncytial virus regulates the host's immune response by modulating the transcription factors NF-kB and STAT1 in bovine cells
Collaborator Contribution Based on our results, Imperial has began investigating the role of the small hydrophobic protein of human respiratory syncytial virus on human cells.
Impact Publication in progress titled "Regulation of the B cell activating factor BAFF by the small hydrophobic protein of respiratory syncytial virus".
Start Year 2017
 
Description Loren Skow, Texas A&M 
Organisation Texas A&M University-Central Texas
PI Contribution We are sequencing extended MHC haplotypes from cattle samples that have previously been typed by low resolution SNPs
Collaborator Contribution Providing samples and SNP data for animals with known phenotypes.
Impact Not yet
Start Year 2017
 
Description Microbial products in insect saliva and effects on virus infection enhancement 
Organisation Boston University School of Medicine
PI Contribution Sharing of results on the discovery of bacterial LPS in Culicoides saliva and its infection enhancing effect on BTV in bovine monocytes. Discussion of future work and comparison of the results to other insect transmitted viruses including those of high importance to humans
Collaborator Contribution Sharing of data from their portfolio on mosquito saliva effects on virus infectivity and replication. Comparison of methodology and discussion of future collaborations
Impact On-going sharing of results and discussion of future experiments
Start Year 2018
 
Description Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan. 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Exchange of technical ideas, reagents and samples.
Collaborator Contribution Provision of field samples and data on avian influenza infection in poultry. This diseases is endemic in poultry in Pakistan.
Impact (1) Increased receptor binding avidity of H9N2 avian influenza viruses is associated with escape from antibody based immunity and enhanced zoonotic potential. presented by Joshua Sealy at GARAD and ZELS conferences in Haneoi, Vietnam from 17-27 January 2018.
Start Year 2008
 
Description Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan. 
Organisation University of Veterinary & Animal Sciences
Department Department of Microbiology
PI Contribution Exchange of technical ideas, reagents and samples.
Collaborator Contribution Provision of field samples and data on avian influenza infection in poultry. This diseases is endemic in poultry in Pakistan.
Impact (1) Increased receptor binding avidity of H9N2 avian influenza viruses is associated with escape from antibody based immunity and enhanced zoonotic potential. presented by Joshua Sealy at GARAD and ZELS conferences in Haneoi, Vietnam from 17-27 January 2018.
Start Year 2008
 
Description Morphological differences of Influenza A viruses 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Exchange of research methods and reagents
Collaborator Contribution (1) Exchange of reagents and Research methods.(2) training of PhD students and Postdoctoral Scientists (3) use of research facilities
Impact Research output were presented at Avian Influenza A virus budding morphology: spherical or filamentous? presented at The European Scientific Working group on Influenza (ESWI) Riga, Latvia. 10 to 13 September 2017 (2) .Budding morphology of avian influenza A viruses. Microbiology Society, annual Conference 2017, 306 April, EICC, Edinburggh, UK
Start Year 2016
 
Description Mucosally Associated Invariant T cells (MAIT) in pig influenza model 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Identified MAIT cells in pig Influenza model. Provide samples from infected and immunised protected animals
Collaborator Contribution Provide expertise in characterising MAIT: development of tetramer; Zell scanner technology allowing high resolution studies of cell phenotype and functions
Impact Joint PhD studentship with Paul Klenerman, University of Oxford
Start Year 2016
 
Description Novel PRRS and swine influenza vaccines 
Organisation National Center for Genetic Engineering and Biotechnology (BIOTEC)
Country Thailand 
Sector Public 
PI Contribution Expertise in PRRS immunology and vaccine evaluation.
Collaborator Contribution Novel vaccine strains and SE Asian field strains
Impact Collaborative grant proposal funded under the BBSRC Newton Fund UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Start Year 2017
 
Description PRRS and Influenza co-nfection studies 
Organisation Government of Thailand
Department National Science and Technology Development Agency (NSTDA)
Country Thailand 
Sector Public 
PI Contribution The Pirbright team of 4 scientists with expertise in immunology and animal care visited Thailand in July 2018 to train Thai colleagues to perform animal experiments (Chiang Mai) and analyse immune responses by ELISPOT and Flow cytometry (Bangkok). This provided Thailand with trained personnel and expertise in testing vaccines in pigs. As a result of the joint work, the Chiang Mai animal facility is currently being renovated. Development of such an animal facility will promote the production and testing of more vaccines by Thai scientists.
Collaborator Contribution Our Thai colleagues provided us with local Thai PRRS and Influenza virus strains which we use in our co-infection studies.
Impact None yet
Start Year 2018
 
Description Partnering with National Taiwan University 
Organisation National Taiwan University
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity".
Collaborator Contribution The partners conceived the project and got in contact about submitting an application for funding together.
Impact An application has been made for a BBSRC-Taiwan Partnering Award in November 2018.
Start Year 2018
 
Description Pathology of BTV in T cell depleted sheep 
Organisation University of Surrey
Department School of Veterinary Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Carrying out animal studies, taking samples for histopathology and sending material
Collaborator Contribution Certified pathologist will attend selected necropsies, sample processing and pathological analysis
Impact Protocols and sample sending established - first clinical trial to start in March 2018
Start Year 2017
 
Description Pathology of bovine skin post Culicoides midge blood-feeding 
Organisation University of Surrey
Department School of Veterinary Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Collection of skin samples from cattle responding with local inflammation to Culicoides biting midges blood feeding, sending of materials
Collaborator Contribution Processing of samples, histopathological analysis, sharing of results
Impact Further identification of cellular and histological local changes in ruminant skin to Culicoides blood-feeing which will inform pathogenesis of co-transmitted arboviruses such as BTV
Start Year 2017
 
Description Paul Digard - The Roslin Insititute 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We generated constructs that express PB1-F2 from a variety of avian influenza strains in eukaryotic expression plasmids. We generated Chimera PB1-F2 expression plasmids where the C-terminus of the PB1-F2 are switched between strains. Using these plasmids we have determined the localization and antagonist activity toward the IFN beta signaling pathway and NF-kb signaling pathway in human and chicken cells. We have therefore provided reagents and intellectual input to the collaboration.
Collaborator Contribution Our collaborators have used these plasmids in there well defined stability assays to determine sequence specific effects on this characteristic. Intellectual input and expertise in a particular assay were made by these collaborators.
Impact We have successfully published some of this work; "The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells." doi: 10.1099/jgv.0.001220. We have an ongoing joint PhD studentship in this area. This collaboration remains current and active.
Start Year 2017
 
Description Professor Sahyan Sharif, University of Guelph, Canada 
Organisation University of Guelph
Department Department of Pathobiology
Country Canada 
Sector Academic/University 
PI Contribution Collaboration between my team and Professor Sharif lead to new finding in Avian immunology, specifically in understanding the effects of nutrients on antigen presenting cells and T cells of chicken. We took the lead in studying the effects of some nutrients (Vitamin D) on the function of chicken T cells, performed most of the experiments in our laboratory. Meanwhile, we participated in studying the effects of Vitamin D on antigen presenting cells of chicken by performing some key experiments because we had access to some specific reagents (monoclonal antibodies) recognizing molecules expressed on chicken antigen presenting cells.
Collaborator Contribution Professor Sharif team took the lead in studying the effects of Vitamin D on antigen presenting cells of chicken, and contributed to experimental plan for studying the effects on chicken T cells.
Impact Two publications in 2015 and 2016: 1. Nitish Boodhoo, Shayan Sharif, Shahriar Behboudi. 1a,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness. PLoS One. 2016 Feb 24;11(2):e0150134 2. Bahram Shojadoost, Shahriar Behboudi, Villanueva AI, Jennifer Brisbin, Ali Ashkar, Shayan Sharif. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 2015 June, 100: 45-51
Start Year 2014
 
Description Re-annotation of Marek's Disease Virus 
Organisation U.S. Department of Agriculture USDA
Department Beltsville Agricultural Research Center
Country United States 
Sector Academic/University 
PI Contribution We are using bioinformatics analysis of high-throughput sequencing data in order to provide a better annotation of the genome of Marek's Disease Virus
Collaborator Contribution Our partners are sharing their extensive expertise of the genomics of MDV, and performing experimental validations
Impact No output yet
Start Year 2015
 
Description Research Collaboration with Nigeria (23rd - 28th January 2017) 
Organisation University of Nigeria
Country Nigeria 
Sector Academic/University 
PI Contribution Analysis of rare breed indigenous chicken in Nigeria.
Collaborator Contribution Helped with sampling and background information of the breed.
Impact Analysis is ongoing.
Start Year 2017
 
Description Semliki Forest 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Generated and analysed RNA-sequencing samples with bespoke in-house methods
Collaborator Contribution Generated samples
Impact The purpose of the collaboration is to study how arboviruses interact with their host cells with modern high-throughput techniques. No outputs yet
Start Year 2017
 
Description Steve Goodbourn - St Georges - chicken innate molecule interactions. 
Organisation Ulster University
Department Biomedical Sciences Research Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution We have collaborated by producing yeast- 2-hybrid plasmids containing the influenza PB1-F2 gene from various avian influenza strains. The PhD student on this studentship then visited Professor Goodbourn's laboratory to carry out the yeast-2-hybrid screen for interaction innate chicken molecules.
Collaborator Contribution Professor Goodbourn's laboratory provided yeast-2-hybrid plasmids containing various innate chicken molecules to be screened for interaction with avian influenza virus PB1-F2 protein. They also offered help and guidance carrying out the interaction technique to understand if PB1-F2 directly associated with chicken innate components. They also provided reporter plasmids and guidance on innate reporter assays in chicken cells.
Impact Successful publication of work. doi: 10.1099/jgv.0.001220. The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells.
Start Year 2016
 
Description Suresh Kuchipudi and Janet Daly 
Organisation Penn State University
Country United States 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Suresh Kuchipudi and Janet Daly 
Organisation University of Nottingham
Department School of Veterinary Medicine and Science Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Swine haplotyping 
Organisation Gift Of Life
PI Contribution Prepared and shipped samples
Collaborator Contribution Sample analysis
Impact Data generation. Not multi-disciplinary
Start Year 2016
 
Description T folicullar helper cells in the pig influenza model 
Organisation University of Oxford
Department Nuffield Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Provide material from influenza infected/vaccinated protected and unprotected animals.
Collaborator Contribution Seph Borrow from Oxford University provide antibodies and advice as to how to identify and charcterise the TFH cells.
Impact Not yet
Start Year 2018
 
Description The GCRF One Health Poultry Hub 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution The focus of the research is to achieve sustainable global intensification of poultry meat and egg production whilst reducing risks to human and animal health and welfare. We are undertaking research to investigate the diversity of avian influenza viruses prevalent in poultry and wild birds in south east Asia.
Collaborator Contribution Royal Veterinary College, London is leading this GCRF One Health Poultry Hub. The project will address the need to meet rising demand for poultry meat and eggs in developing countries, while minimising risk to international public health. Population growth is driving global demand for poultry, meat and egg production; this unfortunately creates conditions in which animal diseases can spread to humans ('zoonoses'). These include bacterial food poisoning and strains with avian influenza with epidemic or pandemic potential. The GCRF One Health Poultry Hub will adopt a 'One Health' approach to the issue of combatting animal-to-human diseases by bringing together a team of laboratory, clinical, veterinary and social scientists. This team will test and evaluate novel interventions. The need for safe poultry production is most urgent in South and South East Asia, so the RVC and its partners will then use their local networks in these regions to put its positive research to immediate use.
Impact Development of international interdisciplinary partnership of 55 investigators from 13 countries to address the emerging global challenges of food security and public health associated with intensification of poultry production.
Start Year 2018
 
Description The effect of the microbiota on immunity to swine infleunza 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution We wish to establish the gut and nasal microbiome in normal healthy pigs and how this is affected by influenza infection. We have collected gut samples from pigs at different stages of infection, as well as nasal swabs which we will provide to our collaborators in Surrey.
Collaborator Contribution Our collaborators in Surrey will perform the sequencing and bioinformatics analysis to determine the microbial communities present in the samples.
Impact No outcomes yet - we are in the process of analysing the samples.
Start Year 2017
 
Description The role of segment 3 in H9N2 avian influenza virus pathogenicity 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution This is a Joint studentship project funded by The Pirbright Institute and The Roslin Institute. We identiifed molecular markers that are responsible for increase in virus pathogenicity and transmission.
Collaborator Contribution The Roslin Institute contributed both intellectually and by proving research training, samples and regents to achieve the prescribed objectives this project.
Impact 1. Oral Presentation Title: Identification of segment 3 sequence polymorphisms which alter shut off activity and pathogenicity of H9N2 avian influenza viruses in vivo. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard and Munir Iqbal. Global Alliance for Research Into Avian Diseases (GARAD). January 17th to 19th 2018. Fortuna Hotel, Lang Ha Street, Hanoi, VietNam 2. Poster title: What is the role of H9N2 avian influenza virus PA-X within an avian model?. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. UK- China CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. August 31st - 1st September 2017. The Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. 3. Poster title: What is the role of H9N2 avian influenza virus PA-X within an avian model?. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. Microbiology Society Annual Conference. April 3rd-6th 2017. Edinburgh International Conference Centre (EICC), Edinburgh, UK. 4. Oral Presentation title: Identification of sequence polymorphisms in segment 3 of H9N2 avian influenza viruses that affect PA-X activity. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. Focused Meeting 2016: Molecular Biology and Pathogenesis of Avian Viruses. September 27th-29th 2016. Charles Darwin House, London, UK. 5. Oral Presentation title: The role of segment 3 in H9N2 avian influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. UK -Taiwan BBSRC Partnering Award workshop: Improving Control of influenza viruses. 26th-27th April 2016. Taipei, Taiwan. 6. Oral Presentation title: Identification of sequence polymorphisms in segment 3 of H9N2 avian influenza viruses that affect PA-X activity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. Microbiology Society Annual Conference 2016; virus Workshop: Negative strand RNA Viruses. 21st-24th March 2016. Arena and Convention Centre, Liverpool, UK. 7. Poster title: The role of segment 3 in H9N2 influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. 7th Orthomyxovirus research conference. 16th-18th September 2015. Ecole Nationale Veterinaire de Toulouse, Toulouse, France. 8. Poster title: The role of segment 3 in H9N2 influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. Edinburgh Infectious Diseases Annual Symposium. 20th May 2015. Royal College of Physicians, Edinburgh, UK.
Start Year 2014
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Agricultural Research Centre
Department Veterinary Serum and Vaccine Research Institute
Country Egypt 
Sector Public 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Imperial College London
Department MSk Lab
Country United Kingdom 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Suez Canal University
Country Egypt 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation University of Tokyo
Department International Research Center for Infectious Diseases
Country Japan 
Sector Hospitals 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Zagazig University
Country Egypt 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Vaccinia 
Organisation Defence Science & Technology Laboratory (DSTL)
Country United Kingdom 
Sector Public 
PI Contribution Generated and analysed DNA- and RNA-sequencing data from vaccinia samples with bespoke in-house pipelines
Collaborator Contribution Generated DNA and RNA vaccinia samples
Impact The collaboration aims to better understand the interaction between vaccina virus and its host(s), through the use of modern high-throughput techniques. No outputs yet
Start Year 2016
 
Title Chicken IFITMs 
Description The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3). 
IP Reference PCT/GB2014/051693 
Protection Patent granted
Year Protection Granted 2018
Licensed Commercial In Confidence
Impact The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3). This patent has enabled us to leverage funding for continued work.
 
Description Poster presentation: International Pig Veterinary Symposium and International PRRS Symposium, Chongqing, PR China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Luke Johnson (PhD student) gave a poster presentation on his studies on the interaction of macrophages and dendritic cells with PRRSV of differing virulence.
Year(s) Of Engagement Activity 2018
 
Description Talk Presented at 2nd International Congress of Veterinary Microbiology, 16th -19th October 2018. Sherwood Breezes Resort-Lara, Antalya, Turkey. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed improving prevention and control of viral diseases of poultry.
Year(s) Of Engagement Activity 2018
 
Description "Challenges for Poultry Industry". Organised by Fakieh Poultry at the Ritz-Carlton Hotel in Jeddah, Saudi Arabia, 2nd -3rd December 2018, 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed prevention and control of infectious diseases affecting poultry production.
Year(s) Of Engagement Activity 2018
 
Description "Interactive session with poultry stakeholders". Title: Overview of Avian Influenza group Research at The Pirbright Institute. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The workshop discussed the economic impacts and prevention strategies against avian influenza viruses affecting poultry production and zoonotic infections.
Year(s) Of Engagement Activity 2018
 
Description 2. Overview of Avian Influenza group Research at The Pirbright Institute. Presented at "Interactive session with poultry stakeholders" at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dissemination of research Project outcomes These include presenting research data for vaccine seed strains with emergent field avian influenza variants; The zoonotic risk posed by emerging avian influenza viruses circulating in the wild birds and poultry. Impact of virus evolution on vaccine efficacy and persistence in poultry.
Year(s) Of Engagement Activity 2018
 
Description A talk at a symposium entitled: Using the 3Rs to support good science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry"
Year(s) Of Engagement Activity 2017
 
Description A talk at the British Poultry Diseases Group meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions.
Year(s) Of Engagement Activity 2017
 
Description AB Improving Approaches to Prevent and Control Viral Diseases of Livestock and Poultry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Batra, A., Maier, H.J., Britton P., Hiscox, J.A., Fife, M.S., 2015. PI3K/AKT signalling during infectious bronchitis virus infection. Food Security: Improving Approaches to Prevent and Control Viral Diseases of Livestock and Poultry, British Council Research Links Workshop, Istanbul, Turkey (Oral presentation)
Year(s) Of Engagement Activity 2015
 
Description AB Society of General Microbiology Annual conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Poster presentation. Batra, A., Maier, H.J., Britton, P., Hiscox, J.A., Fife, M.S. AKT activation during infectious bronchitis virus infection. Society of General Microbiology Annual conference, Liverpool, UK
Year(s) Of Engagement Activity 2013,2014,2015
 
Description BSI antibody meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented work on how we have developed methods to study cattle B cells and antibody responses.
Year(s) Of Engagement Activity 2018
 
Description Bluetongue in wildlife workshop - presentation on "Bluetongue virus in deer (and other wildlife) - pathogenesis and immune responses" July 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Policymakers/politicians
Results and Impact This workshop brought together policy makers, veterinary advisers and scientist to share information and discuss the role of wildlife for important Culicoides-borne viruses of ruminants. Gave 15 minutes presentation on "Bluetongue virus in deer (and other wildlife) - pathogenesis and immune responses"
Year(s) Of Engagement Activity 2018
 
Description Broadcast on African swine fever on BBC wolrd service "The Food Chain" 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interview with BBC World Service "The Food Chain" on African swine fever virus.
Year(s) Of Engagement Activity 2018
URL https://www.bbc.co.uk/programmes/p05xhflj
 
Description CABI_Research activity of Avian Immunology Group at The Pirbright Institute 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Release of research activity within Avian Immunology group at The Pirbright Institute
Year(s) Of Engagement Activity 2018
 
Description Centre of excellence for research on avian diseases (CERAD) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity 2017
 
Description Chair of organising Committee for Wellcome Trust-Animal Genetics and Diseases 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This meeting brought together specialists working on the interface between genomics, genetic engineering and infectious disease with the aims of improving animal and human health and welfare.

Scientific sessions included:
Genetics of immune responses and disease resistance
Genetically engineered livestock (including genome editing)
Quantitative genetics and epigenetics applied to disease
Epidemiology and pathogen evolution
Bioinformatics, comparative and functional genomics
Precision medicine of animal companions
Year(s) Of Engagement Activity 2017
URL https://coursesandconferences.wellcomegenomecampus.org/events/item.aspx?e=635&dm_i=2SUU,HOGH,4R4AW1,...
 
Description Chaired the Poultry Disease group meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Poultry Disease group is a forum of veterinary practitioners to discuss the advances in poultry diseases that could be translated from research labs to the farms
Year(s) Of Engagement Activity 2017
 
Description Cheltenham Science Festival 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Attended the Cheltenham Science Festival and presented "Pandemic Live" an interactive debate on the spread of viruses in livestock. The audience were guided through the decision making processes that accompany an outbreak of an exotic livestock disease outbreak.
Year(s) Of Engagement Activity 2018
 
Description Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines. Presented at as PI of the ZELS project at ZELS grant holders and Stakeholders meeting in Hanoi, Vietnam 23th-24th January 2017. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Engagement and dissemination of research outcome to representatives of research funding bodies such as Department for International Development (DFID), Medical Research Council (MRC) and the researchers working on different research projects funded by BBSRC under Zoonoses and Emerging Livestock Systems (ZELS).
Year(s) Of Engagement Activity 2018
 
Description Conor Haydon participated in the STEM Day - Speed interviews and Careers Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact STEM day engagement and careers
Year(s) Of Engagement Activity 2018
 
Description Coronavirus lecture MSc students (Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A lecture was presented on the replication, cellular interactions and pathogenesis of coronaviruses to 15-20 MSc students from University of Surrey. The students were engaged and interested, answering and asking questions during the session.
Year(s) Of Engagement Activity 2016,2018
 
Description Demonstrating Mathematics (Angita Shrestha) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Angita Shrestha (PhD student within Avian Influenza group) provided help in a Maths session to the new Oxford Doctoral Training Porgramme (DTP) cohort.
Year(s) Of Engagement Activity 2018
 
Description Diamond open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Over 300 people visited our stand at the open day, in which we described the importance of our work for food security and public health. Children from all ages were involved and learned about viruses and played with the huge virus particle that we had produced. One of the major impact was that we described to several people why we work on some viruses that we do not have them in the UK, and the importance of the work for the UK and UK economy and public health was explained.
Year(s) Of Engagement Activity 2017
URL http://www.diamond.ac.uk/Public/VisitUs.html
 
Description Encouraging women into science and engineering STEM. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact MH Visited Warwick School, Redhill to talk to secondary students about careers in STEM. A very positive outcome and well received.
Year(s) Of Engagement Activity 2018
 
Description Evolution of H9N2 avian influenza virus under immune pressure. Presented at 10th International Symposium on Avian Influenza, 15th - 18th April 2018, The Grand Hotel, Brighton, UK. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The primary aim of the workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. In addition, the activity hopes provide information about the emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity 2019
URL https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...
 
Description Expert group meeting for PPR eradication at head quarters of WHO, Vienna, Austria, 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact In the FAO/PPR eradication meeting satya has presented work on PPR epidemiology and diagnosis. The talk was designed for the stakeholders and policy make to make aware on important points that are required for eradication of PPR
Year(s) Of Engagement Activity 2018
 
Description FLI - presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Presentation of current research to the FLI - Germany to build collaborations 19/20 November 2018
Year(s) Of Engagement Activity 2018
 
Description Farnborough futures careers event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The careers event aimed to introduce the range of careers available at Pirbright. In addition I discussed my own career progression and highlighted the range of possible careers that I could have entered. Finally, I discussed some details of the research with interested students. The students had a wide range of backgrounds and career aims but were interested to hear about the Institute and the sort of work opportunities available. Several of them planned to follow up by investigating future job opportunities at Pirbright.
Year(s) Of Engagement Activity 2019
 
Description H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Focus of the conference was to improve control against infectious diseases affecting poultry and livestock.
Year(s) Of Engagement Activity 2018
 
Description H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dissemination of research outcomes to prevent and control of infectious diseases of animals and humans.
- Avian influenza virus evolution and efficacy.
-
Year(s) Of Engagement Activity 2018
 
Description Holt School Surrey. Bee meadow & A-level outreach Talk. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I discussed the importance of pollinators for biodiversity and food security with the younger students. I also discussed careers in science with A-Level students. There was good interaction and many questions surrounding both topics.
Year(s) Of Engagement Activity 2018
 
Description Human Infection Challenge Models Network 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact HIC-Vac is an international network of researchers who are developing human infection challenge (HIC) studies to accelerate the development of vaccines against pathogens of high global impact. The goal is to foster an engaged and interactive community of international researchers to promote open sharing of knowledge and expertise, generate new ideas, support and share best practice, and form new cross-discipline collaborations.
Year(s) Of Engagement Activity 2018
 
Description Immuno-pathobiology of H9N2 avian influenza viruses: looking at how the viruses evolve and persist in poultry. Presented at "Techniques for Healthy Farming and Diseases Prevention & Control of Livestock and Poultry. Beijing, August 13th-15th, 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Dissemination of research outputs. Improvement of vaccines and diagnostics.
Year(s) Of Engagement Activity 2018
 
Description Inauguration of the UK-China Centre of Excellence for Research on Avian Diseases (CERAD) and Symposium on Recent Advances in Avian Disease Research 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Inauguration of the UK-China Centre of Excellence for Research on Avian Diseases (CERAD) and Symposium on Recent Advances in Avian Disease Research. This was held in Binzhou, Shandong province in China in 2015
Year(s) Of Engagement Activity 2015
 
Description Influenza update meeting (Pengxiang Chang) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact presented work entitled: Antigenic characterization of avian influenza H7N9 virus by in vitro immune escape mutant selection method.
Year(s) Of Engagement Activity 2018
 
Description Initiation of a mass vaccination campain ( 2018) in TamilNadu, India 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Scientists from The Pirbright Institute's Vaccine Differentiation group have recently returned from Chennai in India, where they conducted a mass peste des petits ruminants (PPR) vaccination campaign and awareness programme. They joined scientists from four specialist organisations; Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Indian Veterinary Research Institute (IVRI), National Institute of Animal Biotechnology (NIAB) and National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI).
Peste des petits ruminants (PPR), also known as goat plague, is highly contagious and infects small ruminants such as sheep and goats, causing up to 90% mortality. The disease is prevalent across large parts of Africa, the Middle East, India and China and is estimated to cost between US$1.4 billion and US$2.1 billion globally each year. In 2017, the Food and Agricultural Organization (FAO) and the World Organisation for Animal Health (OIE) introduced a global eradication programme to reduce the devastating impact PPR has on the economy and food security of affected countries.
"Over 40 vets joined our vaccination campaign in the Tanir Kulum village of Tiruvallur District, TaminNadu (close to Chennai), where we administered vaccines to over 400 sheep and goats in a single day. We also ran an awareness camp where we provided farmers and vets alike with expert guidance on diagnosing clinical signs of PPR and what measures they could take to reduce its spread", said Professor Satya Parida from Pirbright, who led the collaborative effort with Dr Dhinakar Raj from TANUVAS.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-pes...
 
Description Innovate Guildford Outreach (12th March) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact With amazing exhibits, activities, workshops, talks and competitions, it's FREE to attend. Explore the four zones: Engineering, One Health, Digital Media and Future Living, experience interactive performances, hear inspiring speakers (including leading gaming pioneer Peter Molyneux and animal health pioneer Professor Nick Bacon) and take in an amazing atmosphere. Theatre, music and comedy will feature through the day, with everything from how technology might have affected Shakespeare's plays to risky recycling and photographic secrets.
Year(s) Of Engagement Activity 2017
URL https://www.guildford.gov.uk/innovateguildford
 
Description Inreview with Daily Telegraph about African swine fever 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Interview with reporter from Daily Telegraph about African swine fever virus and risks to UK farmers
Year(s) Of Engagement Activity 2018
URL https://www.telegraph.co.uk/news/2018/12/12/uk-farmers-warned-alert-african-swine-fever-virus-hits-e...
 
Description International Avian Respiratory Disease Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Michael presented his research at the 2018 International Avian Respiratory Disease Conference in the US to an audience of avian researchers and representatives form the poultry veterinary vaccine industry. This sparked discussion and potential future collaborations
Year(s) Of Engagement Activity 2018
 
Description International Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Global Alliance for Research on Avian Diseases conference health in Hanoi 17-19 January 2018. Purpose of the conference was to establish global partnerships and links between academic researchers and the poultry industry to improve joined up work in regards to preventing, controlling and
combating avian diseases. The conferences provided a forum for discussion and exchange of ideas around the single theme of exploiting available opportunities to deal with emerging and existing infectious avian diseases in poultry production systems and to safeguard food supplies and human health.
Conference speakers discuused basic, applied and commercial aspects of research on avian diseases including:
• Evolution and Epidemiology of Avian Pathogens
• Host-Pathogen Interactions - Virulence and Pathogenicity
• Pathogen Mechanisms of Immune Evasion
• Next Generation Disease Control Strategies
• Vaccines
• Immunity
• Diagnostics
• Zoonoses and Socio-economic impacts of avian diseases
• Global Research Opportunities and Threats
Year(s) Of Engagement Activity 2018
URL http://www.theeventsportal.com/GARAD-2018/3/Home
 
Description International Day of Women and Girls in Science 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I gave a quote as a STEM ambassador about why I think it is important to make sure women and girls have the same opportunities in STEM. This was shared on social media to celebrate International Day of Women and Girls in Science, a UN-led initiative.
Year(s) Of Engagement Activity 2019
 
Description International Women's Day video for social media 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Three team members were interviewed about their careers in science for International Women's Day. The videos were posted on Pirbright's website, Facebook and Twitter to celebrate what we love about working in science.
Year(s) Of Engagement Activity 2018
 
Description Interview with CNN on African swine fever virus 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interview with Nina Avramova from CNN International about African swine fever virus generally including transmission mechanisms and control strategies
Year(s) Of Engagement Activity 2018
URL https://edition.cnn.com/2018/10/02/health/african-swine-fever-europe-china-spread-intl/index.html
 
Description Interview with Health for Animals about African swine fever vrius vaccine development and current situation 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Interview with journalists from Health for Animals about African swine fever vaccine research published in a newsletter sent to 200 people.
Year(s) Of Engagement Activity 2018
URL https://healthforanimals.org/resources-and-events/newsletter-repository/17-disease-outbreaks.html?q=...
 
Description Interview with IEG Policay about ASFV vaccines 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Interview with Peter Rixon of IEG policy about the prospects for development of vaccines for African swine fever vrius
Year(s) Of Engagement Activity 2018
URL https://iegvu.agribusinessintelligence.informa.com/CO221819/ASF-outbreak-in-Belgium-highlights-lack-...
 
Description Interview with Journalist from Science Journal News Section 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interview with Science Journal journalist resulted in publication in news section http://www.sciencemag.org/news/2017/12/african-swine-fever-outbreak-alarms-wildlife-biologists-and-veterinarians
Year(s) Of Engagement Activity 2017
URL http://www.sciencemag.org/news/2017/12/african-swine-fever-outbreak-alarms-wildlife-biologists-and-v...
 
Description Invitation to give keynote talk on 'Control of avian diseases: Challenges and opportunities' 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave the Keynote talk on 'Control of avian diseases: Challenges and opportunities' at this International Symposium organized with an aim to gather international virology experts from both human and veterinary fields to share research experience, update findings and contribute their expertise through presentation and interactive discussion. The topics will cover a wide range of virology research common in both human and animal aspects. In addition, technological platforms such as vaccine development, and novel assays will be emphasized and addressed.
Year(s) Of Engagement Activity 2018
URL http://www.biotec.or.th/virus-symposium2018/index.php
 
Description Invitation to talk at the Vaccine Symposium "Vaccines at different levels of complexity from subunit to whole cell formulations" jointly organised by the Centre of Genomic Regulation (CRG), Radboud UMC and MSD Animal Health, with the support from the MycoSynVac project 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave a talk and participated in the discussion on the challenges and research opportunities for innovation in poultry disease control
Year(s) Of Engagement Activity 2017
URL http://www.crg.eu/
 
Description Invited Keynote Speaker International Society of Veterinary Epidemiology and Ecology, China Mai Thailand 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote talk on progress towards African swin efever virus vaccine development at ISVEE meeting Thailand
Year(s) Of Engagement Activity 2018
 
Description Invited oral presentation at 18th National Conference for Pig Disease Prevention and Eradication, Wuhan, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited oral presentation: 'Trying to hit a moving target: Novel approaches to porcine reproductive and respiratory syndrome virus vaccine development', 18th National Conference for Pig Disease Prevention and Eradication, Wuhan, China
Year(s) Of Engagement Activity 2018
 
Description Invited speaker British Society of Veterinary Pathology Annual Meeting 2018, Applied Ruminant Pathology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited speaker on "Bluetongue virus and Schmallenberg virus - recognising the continued threat of Culicoides-borne viruses of ruminants" British Society of Veterinary Pathology Annual Meeting 2018, Applied Ruminant Pathology
School of Veterinary Medicine, University of Surrey, 29th of September 2018,
Year(s) Of Engagement Activity 2018
URL https://www.bsvp.org/event/applied-ruminant-pathology-disease-investigation-diagnosis-and-surveillan...
 
Description Invited speaker VECTOR-BORNE DISEASES (VBD) IN THE UK - BIENNIAL MEETING 2018 John Innes Centre Norwich 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited speaker on "The Host-vector-pathogen Interface of Culicoides-borne Bluetongue Virus" at the VECTOR-BORNE DISEASES (VBD) IN THE UK - BIENNIAL MEETING 2018 John Innes Centre Norwich 4th-5th December 2018
Year(s) Of Engagement Activity 2018
 
Description Invited speaker at Imperial College 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Gave a talk on our recent research in swine influenza. Discussions with PIs at Imperial after the talk. Established collaborations.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker at the British VeterinaryPoultry Association 2018 meeting at Boehringer Ingelheim 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact I was invited to present my research findings at the British Veterinary Poultry Association 2018 meeting, held at Boehringer Ingelheim.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker, Merseyside BSI Affinity group 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I described the methods we used to identifyTissue resident memory cells in pigs, which sparked a lively discussion and interest in the technique. As a result I have established two collaborations with colleagues from Liverpool.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker, Surrey University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Under- and postgraduate students attended a talk I gave at the Department of Biological Sciences in Surrey. There was a lively discussion afterwards and as a result I have established a new collaboration.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker, Vienna Veterinary School 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Gave a guest lecture at the Veterinary school in Vienna. There were a lot of questions and discussion after the talk. As a result established collaboration with Gerner Wilhelm's group.
Year(s) Of Engagement Activity 2019
URL https://www.vetmeduni.ac.at/de/graduate-school-pig-and-poultry-medicine/
 
Description Invited talk at Agricultural Science congress at New Delhi, India, February-2019- Use of reverse genetics to study the early pathogenesis and to develop marker vaccines for PPR 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact 1. In a DBT-BBSRC FADH grant, The Pirbright Institute in collaboration with four Indian partners (TANUVAS, IVRI, NIAB and NIVEDI) has developed a PPR vaccine, which will be the first to differentiate between infected and vaccinated animals (DIVA), for which a patent application has been filed. Scientists have manipulated in the genome of PPR full length cDNA and rescued the DIVA vaccine virus using reverse genetic technique. Further this vaccine has been tested in goats providing full safety and potency upon virulent virus challenge. In contrast to current vaccine, this new DIVA vaccine can differentiate between naturally infected and vaccinated animals, therefore will help in meaningful assessment of vaccine coverage and epidemiological surveillance based on serology, in turn increasing the efficiency of control programmes.
2. Little is known about the early events in the development of peste des petits ruminants (PPR) virus (PPRV) infection. The current dogma is that similar to RPV, PPRV replicates primarily in the epithelium of the respiratory tract before disseminating throughout the host. However, our investigations, using intranasal inoculation of PPRV containing GFP (Green Fluorescent Protein) to mimic natural infection, indicate this is not the case and that immune cells in the pharyngeal tonsil is the primary target. This is a ground breaking discovery which changes the PPR pathogenesis. This infectious GFP virus is made from a synthetic cDNA of field virus (PPRV/Mor-08) using reverse genetics technique under FADH grant.
The presentation sparked questions and discussions after the talk. Two PPR DIVA vaccines developed and the mechanism of PPR virus pathogenesis explained.
Year(s) Of Engagement Activity 2019
 
Description Invited talk at the National Institute for Animal Biotechnology, Hyderabad on 3rd Jan 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Participants were engaged in the recent advances in biotechnology, challenges and opportunities.
Year(s) Of Engagement Activity 2019
 
Description Invited to attend and present at the RSPCA Lay members Forum, Royal Society London 10th of December 2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Third sector organisations
Results and Impact Invited to attend to the RSPCA Lay members Forum, Royal Society London 10th of December 2018 and present on the ethical review process of animal research
Year(s) Of Engagement Activity 2018
URL https://science.rspca.org.uk/sciencegroup/researchanimals/ethicalreview/differentsystems/uk/events
 
Description Invited to talk at the International Conference on Building Human and Animal Health Capacities organised by the Jordan University of Science and Technology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Participated in the discussion and talked on 'The recent advances in vaccination-based control of poultry diseases'. The need for increased awareness of the pathogen diversity in the design of vaccines and the opportunities from advances in vaccine technologies were discussed
Year(s) Of Engagement Activity 2017
URL http://www.just.edu.jo/conferences/bhahc/Pages/default.aspx
 
Description Keynote talk at the World Veterinary Poultry Association meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited to give the keynote talk on 'Avian tumour viruses: their contribution to our understanding of cancer' at the World Veterinary Poultry Association XXth Congress in Edinburgh
Year(s) Of Engagement Activity 2017
URL http://www.wvpac2017.com/
 
Description Kingdown School Warminster Wiltshire talk to 6th form students about career in science. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I presented my career path to the students to engage them on STEM careers. I had a good level of discussion and many questions.
Year(s) Of Engagement Activity 2018
 
Description Laboratory Science Animal association (LASA) meeting in Birmingham, Invited speaker 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Gave a presentation on the tolls we have developed to study swine influenza
Year(s) Of Engagement Activity 2018
URL http://www.lasa.co.uk/meetings/
 
Description Lecture on entereic coronaviruses to MSc Students (Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A lecture was presented on the pathology, diagnosis and detection of enteric coronaviruses, including virus replication and interaction with the host. The students were engaged and asked/answered question throughout the session. We were invited to repeat the lecture for the subsequent student intakes.
Year(s) Of Engagement Activity 2015,2016,2018
 
Description Lecture to MSc studnets University of Surrey 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Lecture on African swine fever virus to MSc students University of Surrey Veterinary faculty
Year(s) Of Engagement Activity 2018
 
Description MBPAV -presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Molecular Biology and Pathogenesis of avian viruses conference - Oxford 3-4th September 2018. Oral presentation given on drug resistant influenza viruses to approximately 50 scientist. Increased collaborative approaches following presentation.
Year(s) Of Engagement Activity 2018
URL https://microbiologysociety.org/event/society-events-and-meetings/focused-meeting-2018-molecular-bio...
 
Description MDV conference Tours 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact During the 11th International Symposium on Marek's Disease and Avian Herpesviruses, which took place from 6 to 9 July 2016, Paolo Ribeca gave a talk on "Attenuation of MDV: an RNA-seq based perspective".
Year(s) Of Engagement Activity 2016
 
Description MF AIRG 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Avina immunology research group meeting in Canada
Year(s) Of Engagement Activity 2014
 
Description MF PAG 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The Plant and Animal Genome XXIV Conference (PAG) is designed to provide a forum on recent developments and future plans for plant and animal genome projects. Consisting of technical presentations, poster sessions, exhibits and workshops, the conference is an excellent opportunity to exchange ideas and applications on this internationally important project.
Year(s) Of Engagement Activity 2014,2016
 
Description MF STEM3 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Students from Years 5-6 i. Gave a talk on the Ebola virus outbreak in west Africa. Porvided virus and DNA models, careers hand-outs.
Year(s) Of Engagement Activity 2016
 
Description MSc student visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I hosted several MSc students from University of Surrey, discussed avian research with them and demonstrated some laboratory techniques. The students gained an understanding of academic research and laboratory work.
Year(s) Of Engagement Activity 2018,2019
 
Description Mapping antigenic determinants of H9N2 avian influenza viruses and improving vaccines and diagnostics" presented at Foreign Talent Lecture: New strategies and new techniques for the prevention and control of major avian diseases. Shandong Binzhou Animal Science & Veterinary Medicine Academy, Binzhou, China. 6th August 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The focus of the meeting was to improve the control systems against poultry diseases including development of improved vaccines and diagnostics
Year(s) Of Engagement Activity 2018
 
Description Microbiology Society Annual conference (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Poster or oral presentation at Microbiology Society annual conference, a major international event. The participants included researchers from many different areas of science. This provides a platform to promote research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014
 
Description Microbiology Society Avian Focus Meeting (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I gave a 15 minute oral presentation to an audience of around 80 people in 2016 and 2018. The audience consisted of researchers from many different scientific institutions from different areas of avian research. This was a great opportunity to present to experts in this field and gain novel insights into the project and the possible applications of my work.
Year(s) Of Engagement Activity 2016,2018
 
Description NL Animal Health Research Club 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact ARC will hold up to two dissemination events per annum. The aim of these events is to facilitate the dissemination of the research findings from the grants awarded through ARC to the Industry Members of the Club and other researchers. The dissemination events also provide an excellent opportunity for networking and we encourage delegates to make the most of the time made available for this purpose in the programme. BBSRC staff and the ARC coordinators will be available throughout the day should you have any questions regarding the Club.
Presentations: The presentations are the main opportunity for ARC research groups to present research findings and future plans and for Industrial Members to find out about ARC funded projects and provide feedback. Featured projects have been allocated presentation time as described in the programme and should include at least 5 minutes for questions. Presenters are asked to keep to time and presentations should demonstrate the relevance of the work to animal health and the livestock industry.
Posters: Research groups funded through ARC have been offered the opportunity to provide posters of work which is of relevance to the Industry Members of the Club. This will provide an opportunity to have informal discussions about current progress during networking activities and provide additional project information of relevance to ARC.
Year(s) Of Engagement Activity 2015,2016
 
Description Networking presentation-INRA, Paris, France 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A presentation at INRA describing the role of the SH protein of BRSV in modulating immune responses in calves. This presentation resulted in a heated debate on the role of viral proteins in immune evasion and their activities in attenuated vaccines.
Year(s) Of Engagement Activity 2017
 
Description Newton Agham Researcher Links Workshop on "Novel Vaccines and Diagnostic Technologies Against Emerging and Re-emerging Veterinary Pathogens, Rizal Park Hotel, Manila, Philippines 4-7 February 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The primary aim of this workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. The workshop provided information on emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity 2019
URL https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...
 
Description Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I presented my work at the Nidovirus Symposium, which is held every three years and brings together researchers from industry and academia from many different countries. I had some interesting discussions with other researchers and built my network.
Year(s) Of Engagement Activity 2017
 
Description Oral and poster presentation at European Society of veterinary Virology and EPIZONE (ESVV/EPIZONE) at Vienna, Austria 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation has been made on early events of PPR virus pathogenesis. The presentation reveled that PPR virus first taken up by the immune cells to the Pharyngeal tonsil and then to other lymph nodes before generalisation of the disease. Secondary replication happens in epithelial cells. This alters the existing believe that virus primarily replicate in the epithelial cells.
Similarly a poster presentation has been done to show the detection of nucleic acid of PPR virus during PPR eradication programme, nasal sample has been shown as the best sample for viral genome detection.
Year(s) Of Engagement Activity 2018
 
Description Oral presentation at the Global Alliance for Research on Avian Disease meeting in Hanoi, Vietnam 15th -19th Jan 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact A talk was given on the PB1-F2 function in avian influenza infection of avian hosts at the GARAD meeting. This meeting was attended by approximately 120 researchers and poultry industry members from a wide international geographic region.
Year(s) Of Engagement Activity 2018
 
Description Oral presentation to Large Animal Research Network 24th May 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Talk about work understanding PB1-F2 function in avian hosts highlighting how the animal work carried out was undertaken and monitored. this was delivered to an audience of researchers, funders and industry interested in animal model systems for research and the 3Rs commitment surrounding this work. Discussions with interested parties about our chicken work at the Institute and the diverse way that the birds are entertained whilst being housed (e.g. live food, vegetables, perches, dust baths etc).
Year(s) Of Engagement Activity 2017
 
Description Organisation of Influenza meeting at Roslin Institute 21st-22nd November 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Along with Paul Digard (Edinburgh) and Edward Hutchinson (Glasgow) I helped to organize, plan and run a Influenza update meeting which ran for 2 days at The Roslin Institute in November 2017. The symposium was attended by approximately 60 influenza researchers from the UK. Talks were given by a range of people with student talks and posters being awarded prizes. This was supported by Medimmune and the Microbiology Society.
Year(s) Of Engagement Activity 2017
 
Description Organisation of UK-India Bioinformatics Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The meeting discussed the challenges and opportunities and have decided to come up with a strategy document on working together between UK and India on data-driven biology
Year(s) Of Engagement Activity 2018
 
Description Organised the 3rd UK-China CERAD meeting in Guildford, UK in July 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The 3rd UK-China CERAD meeting helped to bring together professional from both countries to identify challenges and exploit the opportunities for improved control of poultry diseases and increasing food security.
Year(s) Of Engagement Activity 2017
URL http://www.uk-china-cerad.org/
 
Description Organiser of the Animal Genetics and Diseases conference 08 - 10 May 2019 Wellcome Genome Campus, UK. Highlighting recent advances in animal genetics and genomic technologies. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I am on the organising committee for this conference. The second conference in this series will highlight recent advances in animal genetics and genomic technologies. It will bring together specialists working on the interface of genomics, genetic engineering and infectious disease with the aims of improving animal and human health and welfare.

Novel genomic technologies, mathematical modelling and quantitative genetics approaches, applied to host animals, as well as their pathogens, have transformed the understanding of animal diseases, host-pathogen interactions and epidemiology and their effects on productivity of farmed animal species and food supply chains.

This year's conference will not only put the spotlight on the immune response of host animals and epidemiology but also cover the genetics and genomics of pathogens and the impact of animal-human relationships.

We encourage registrations from researchers, breeders and technical specialists interested in learning and disseminating the latest cutting-edge techniques and methodologies across model species, wildlife, farmed animals and companion animals.
Year(s) Of Engagement Activity 2019
URL https://coursesandconferences.wellcomegenomecampus.org/our-events/animal-genetics-diseases-2019/?dm_...
 
Description Organising and giving Keynote talk at the GARAD meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Global Alliance of Poultry industry professional and academia to discuss about the advances in control strategies of diseases
Year(s) Of Engagement Activity 2018
URL http://Garad.org
 
Description PPR eradication meeting organised by FAO and OIE at Rome and talk has been delived on Development of DIVA vaccines 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Satya Parida is working as an expert to PPR eradication and he is being invited for few of FAO/OIE meetings. The meetings discussed about roadmap, vaccine production capacity and eradication policy.
Satya also presented work on PPR DIVA vaccine and DIVA tests developments and their evaluation in goats.
Year(s) Of Engagement Activity 2018
 
Description PacBio Leiden 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presented data to academics and industry experts on the methods we have developed to enrich and sequence targeted areas of genomes
Year(s) Of Engagement Activity 2018
 
Description Participation in Understanding Animal Research Video 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interviewed and filmed by Understanding Animal Research as part of their 360 degree tour of UK animal facilities (http://www.labanimaltour.org/)
Year(s) Of Engagement Activity 2017
URL http://www.labanimaltour.org/
 
Description Pig and Poultry Fair 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Discussed Institute research with pig and poultry farmers, related industry and the general public. Received requests for more information related to research, business and studentships.
Year(s) Of Engagement Activity 2018
URL https://www.pigandpoultry.org.uk/
 
Description Postdocteral scientist - oral presentation at the 12th Annual meeting of Epizone (joint with 11th International Congress for Veterinary Virology, ESVV 2018) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Postdoctoral scientists abstract submission was selected for oral presentation. Project results obtained at the time were presented to a highly interested and engaging audience of more than 250 scientists, veterinarians, students and policymakers.
Year(s) Of Engagement Activity 2018
URL https://www.epizone-eu.net/en/Home/News/show/Abstract-book-EPIZONEESVV-meeting-now-online-.htm
 
Description Poster by Nick on the sequencing of Zimbabwe FMDV 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Nick Knowles presented a poster at the 2018 Europic (the meeting of the European Study Group on the Molecular Biology of Picornaviruses) on "Complete Genome Sequence Analysis of Over 140 Foot-and-mouth Disease Viruses Isolated From Free-living African Buffalo (Syncerus Caffer) in Zimbabwe" on behalf of Jemma Wadsworth, Bruce Bolt, Luca Ferretti, Euan C. Anderson, Ashley Gray, Paolo Ribeca, and himself. The sequencing was funded by the Transboundary Pathogens portal project awarded to Pirbright.
Year(s) Of Engagement Activity 2018
 
Description Poster presentation by PhD student and seior postdoc at SGM meeting and Negative Strand Virus (NSV) meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact The work on early events on Pathogenesis of PPR virus has been presented that showed PPR virus primarily replicates in Tonsils, not in the mucosal epithelium as thought till date.
Year(s) Of Engagement Activity 2018
 
Description Poster presentation: European Veterinary Immunology Workshop, Utrecht, The Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Luke Johnson (PhD student) gave a poster presentation on his studies on the interaction of macrophages and dendritic cells with PRRSV of differing virulence.
Year(s) Of Engagement Activity 2018
 
Description Poultry Health course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poultry Health Course is run as a residential and online course to transfer recent advances in avian disease research from the laboratory to the field.
Year(s) Of Engagement Activity Pre-2006
URL http://www.poultryhealthcourse.com/
 
Description Presentation at Global Alliance for Research on Avian Diseases Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation of my research at the Global Alliance for Research on Avian Diseases Conference in Vietnam to approximately 80 conference participants resulted in discussions about future research directions.
Year(s) Of Engagement Activity 2018
 
Description Presentation at RIVR meetings 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Presentation of my research at Recently Independent Virology Researchers (RIVR) meetings in 2016, 2017, 2018 and 2019. I discussed my work and future collaborations with other virology researchers working in the UK.
Year(s) Of Engagement Activity 2016,2017,2018,2019
 
Description Presentation at Transboundary disease course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Discussion of BTV transmission, pathology and clinical disease to international participants of the Transboundary disease course
Year(s) Of Engagement Activity 2017
 
Description Presentation at the EU BTV reference laboratory meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Presentation of BTV pathogenesis and effects of Culicoides saliva to representatives from all European reference laboratories plus some additional participants from the EU and industry
Year(s) Of Engagement Activity 2017
 
Description Presentation of project results at the 13th International dsRNA Virus Symposium 24th-28th of September 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster and Oral presentations included key results of the studentship project
Year(s) Of Engagement Activity 2018
 
Description Presentation to BBSRC Executive Board 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact I presented our research to the BBSRC Executive Board when they visited The Pirbright Institute in September 2018 and discussed the impacts of our work..
Year(s) Of Engagement Activity 2018
 
Description Presentation: BSI AGM: Immune regulation by the SH protein of BRSV 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation at the BSI's AGM to describe the data generated regarding the role of the SH protein of BRSV in regulating immune responses after natural infections. Initiated a debate about the role of the SH protein of human RSV in immune regulation.
Year(s) Of Engagement Activity 2017
 
Description Project close meeting at TANUVAS, Chennai July 2018- Delivering talk on outcome of the project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact The project team held their final workshop at TANUVAS, which concluded a four year Farmed Animal Disease and Health (FADH) grant joint funded by the Biotechnology and Biological Sciences Research Council (BBSRC) and the Indian Government's Department of Biotechnology (DBT). "This grant has enabled us to collaborate with four organisations across India, and ensured that our research can be applied in the field to aid the campaign for PPR global eradication", said Professor Parida.The project covered many areas of research which are essential for understanding PPR and creating tools to help control and prevent the disease. The team have now filed a patent application for their newly developed PPR vaccine, which is the first to differentiate between vaccinated and infected animals (DIVA) - a quality that enables livestock owners to protect their animals whilst continuing to trade.
The team have also investigated how the PPR virus (PPRV) infects sheep and goats and how their immune systems respond. By inserting green fluorescent protein into virulent PPRV and administering the modified virus to goats, they demonstrated that PPRV primarily infects the tonsils, challenging the earlier belief that the virus first replicates in the respiratory tract epithelial cells. The collaborative project has also generated better diagnostic tests for use in the field and laboratory, and preliminary research has identified why some Indian breeds of goats and sheep are resistant to the disease, which could help scientists to create PPRV resistant breeds in the future.
Project partners, scientists from the University and 40 field veterinarians have joined the meeting. An awareness training has been conducted on PPR disease and eradication for these field veterinarians.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-pes...
 
Description Provided information to Podcast This week in Virology about African swine fever virus spread in wild boar 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact E-mail correspondence providing advice to presenters of TWIV This week in Virology Podcast.
Year(s) Of Engagement Activity 2019
URL http://www.microbe.tv/twiv/twiv-532/
 
Description Represented The Pirbright Institute at the British Pig and Poultry Fair, 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Dr Andrew Broadbent represented The Pirbright Institute at the British Pig and Poultry Fair, 2019 that was well attended by members of the General Public, poultry industry representatives and veterinary practitioners.
Year(s) Of Engagement Activity 2018
URL https://www.pigandpoultry.org.uk/
 
Description Reverse genetics seminar (University of Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A seminar was given based upon the reverse genetics system of infectious bronchitis virus. The audience was a group of 10 -15 MSc students from University of Surrey. The students were engaged, asked questions about the research and careers in science. We were asked to repeat the seminar for the next year's intake of students.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017
 
Description Reverse genetics seminar to MSc students 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact A seminar was presented describing reverse genetics of viruses, including infectious bronchitis virus and the application of reverse genetics in research. The students were interested and engaged, participating in activities and asking questions. We were invited to present the seminar in subsequent years for new student intakes.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2018
 
Description STEM Outreach Event (Farnborough, 9th November) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact See web link
Year(s) Of Engagement Activity 2016
 
Description Second Symposium of the UK-China Centre of Excellence for Research on Avian Diseases held in March 2016 in Binzhou, Shandong Province, China 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The second international meeting of the UK-China CERAD was attended by a number of delegates from both UK and China and reviewed the progress. Plans were drawn of further activities and plans for securing additional grants.
Year(s) Of Engagement Activity 2016
 
Description Seminar given at The Roslin Institute - 19th October 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact Invited to give a Seminar on PB1-F2 programme of work at The Roslin Institute in Edinburgh to researchers.
Year(s) Of Engagement Activity 2017
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar at Beijing Institute of Animal Sciences, Chinese Academy of Agricultural Sciences 12 Zhongguanchan South Stree, Haifing District Beijing
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presentation on African swine fever virus vaccine development to Yebio Bioengineering and China Animal Health and Epidemiology Centre, Qingdao, PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar on African swine fever virus host interactions and vaccine development. Yangzhou University Veterinary Faculty PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus virus host interaction and vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus virus host interaction and vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Seminar on African swine fever virus evasion of host defences and vaccine development Institute of Molecular and Cellular Biology, UCL London
Year(s) Of Engagement Activity 2018
 
Description Seminar on prospects for African swine fever virus vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar at Harbin Institute for Veterinary Research, PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on virus host interactions and prospects for African swine fever virus vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Seminar on African swine fever virus host interactions and vaccine development
Year(s) Of Engagement Activity 2018
 
Description Sue Baigent participated in the Teen Tech event on 22nd Jan 2019 on General Science engagement 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact A General Science event
Year(s) Of Engagement Activity 2019
 
Description Sue Baigent participated in the Winston Churchill Careers Fair on 7th Nov 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Careers fair
Year(s) Of Engagement Activity 2018
 
Description Surrey County Show 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact The Surrey County Show is an agricultural show in Guildford that is visited by the general public. The Pirbright Institute had a stand with various activities for visitors to take part in and explain our science to a wide audience. Giulia spent a day working on the stand and spoke with many children and adults about her work at Pirbright to change public attitudes and stimulate increased interest in research.
Year(s) Of Engagement Activity 2018
 
Description Surrey Open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Described the research activity of the Pirbright Institute for students from school, graduate and postgraduate students, and provided information on research facilities in the institute.
Year(s) Of Engagement Activity 2017
URL https://www.surrey.ac.uk/opendays/vet
 
Description Surrey vet school open day - MJB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact The event was an open day for prospective students for the University of Surrey vet school. The course includes a research placement, which can be conducted at Pirbright. Therefore there was a stand from Pirbright to describe the research we perform generally as well as a discussion of our specific research. The audience were generally interested and the information helped inform them when selecting a university course.
Year(s) Of Engagement Activity 2018
 
Description TW ARC 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact ARC will hold up to two dissemination events per annum. The aim of these events is to facilitate the dissemination of the research findings from the grants awarded through ARC to the Industry Members of the Club and other researchers. The dissemination events also provide an excellent opportunity for networking and we encourage delegates to make the most of the time made available for this purpose in the programme. BBSRC staff and the ARC coordinators will be available throughout the day should you have any questions regarding the Club.
Presentations: The presentations are the main opportunity for ARC research groups to present research findings and future plans and for Industrial Members to find out about ARC funded projects and provide feedback. Featured projects have been allocated presentation time as described in the programme and should include at least 5 minutes for questions. Presenters are asked to keep to time and presentations should demonstrate the relevance of the work to animal health and the livestock industry.
Posters: Research groups funded through ARC have been offered the opportunity to provide posters of work which is of relevance to the Industry Members of the Club. This will provide an opportunity to have informal discussions about current progress during networking activities and provide additional project information of relevance to ARC.
Year(s) Of Engagement Activity 2015,2016
 
Description Taiwan -UK Partnering Award: Surveillance of influenza viruses 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Attended the International Flu Virus Symposium for Celebrating IPM 47th Anniversary" in Tri-Service General Hospital in Taipei, Taiwan.

Tri-Service General Hospital address:
No. 325, Sec. 2, Chenggong Rd., Neihu Dist., Taipei City 114, Taiwan (R.O.C.)

Invited speaker for medical staff and research scientists.
Year(s) Of Engagement Activity 2019
URL https://www.cdc.gov.tw/rwd/english
 
Description Talk as part of the University of Veterinary Medicine (Vienna) doctoral school "Infectious Diseases of Pig and Poultry" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Talked about the Pirbright Institute's work on African swine fever virus to researches in Veterinary Medicine (Virology and Immunology) in Vienna.
Year(s) Of Engagement Activity 2018
 
Description Talk by Nick on the sequencing of Zimbabwe FMDV 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Nick Knowles gave a talk at the 2018 Open Session of EuFMD (European commission for the control of foot-and-mouth disease ) on "Complete Genome Sequence Analysis of Over 140 Foot-and-mouth Disease Viruses Isolated From Free-living African Buffalo (Syncerus Caffer) in Zimbabwe" on behalf of Jemma Wadsworth, Bruce Bolt, Luca Ferretti, Euan C. Anderson, Ashley Gray, Paolo Ribeca, and himself. The sequencing was funded by the Transboundary Pathogens portal project awarded to Pirbright.
Year(s) Of Engagement Activity 2018
 
Description Teaching- lecturing, small group classroom teaching and laboratory practical teaching 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact I have been actively engaged with teaching activities at the University of Surrey, giving lectures, small group classes and laboratory practical classes to vet students and MSc students.
Year(s) Of Engagement Activity 2015,2016,2017
 
Description Teentech 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The annual Teentech student science event in Guildford aims to encourage teenagers to consider science subjects and science careers. Our stand had considerable interest from the schoolchildren who were very enthusiastic. The event stimulated an increased interest in science and research.
Year(s) Of Engagement Activity 2016,2019
 
Description The Hurst careers event - HJM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The Hurst school held a careers event for GCSE students to attend throughout the day. The Pirbright stand contained a variety of engagement activities to demonstrate the research we perform and to stimulate discussion. The students seemed interested in the activities and those more interested in a science career asked a variety of questions about the research and entry into scientific research as a career.
Year(s) Of Engagement Activity 2018
 
Description The Pirbright Village Duck Fair held on 16 June 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Local Village Fair, interaction with local residence to expanse research aims and activities of The Pirbright Institute and benefits to UK and around the globe to prevent and control infectious diseases of animals and humans.
Year(s) Of Engagement Activity 2018
 
Description Tillingbourne bug hunt. New Rd, Chilworth, Guildford GU4 8NB 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research. Good interest and engagement.
Year(s) Of Engagement Activity 2018
 
Description Tour of Pirbright Research facilities for CLA group 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The CLA is the membership organisation for owners of land, property and businesses in rural England and Wales. The CLA came for a tour of the Jenner building facilities at The Pirbright Institute. I led a tour of our building for the group and described the work undertaken by the avian research programme here.
Year(s) Of Engagement Activity 2017
 
Description U3A talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Presentation on influenza virus research at The Pirbright Institute for the local University of the Third Age group, approx. 50 people aged 65 years + all with an interest in science.
Year(s) Of Engagement Activity 2018
URL https://cu3asandt.wordpress.com/2018/09/23/meeting-2nd-october/
 
Description UK-China Philippines-Thailand Swine and Poultry Research Initiative project kick-off workshop" at China Agriculture University, Beijing, 26th-28th September 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Emerging new approaches were discussed for prevention and control of infectious diseases affecting swines and poultry.
Year(s) Of Engagement Activity 2018
 
Description UK-India bioinformatics workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact On February 9 2018 Pirbright organised a "UK-India bioinformatics workshop" in order to foster ties between Indian and UK bioinformaticians working in the field of livestock and crop research. Paolo Ribeca gave a talk on "Bioinformatics and sequencing at Pirbright".
Year(s) Of Engagement Activity 2018
 
Description Understanding Antigenic Diversity, Zoonotic potential and immunological prevention of avian influenza viruses affecting poultry. Presented at "UK-China Philippines-Thailand Swine and Poultry Research Initiative project kick-off workshop" at China Agriculture University, Beijing, 26th-28th September 2018. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Meeting focus was to share research plans funded under "UK-China-Philippines-Thailand Swine and Poultry Research Initiative". Research projects investigate pathogens, zoonotic pathogens or "commensals" associated with food safety issues affecting poultry and swine.
Year(s) Of Engagement Activity 2018
URL https://bbsrc.ukri.org/funding/filter/joint-swine-poultry-initiative/
 
Description University of Surrey School of Veterinary Medicine Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Presentation of work conducted at The Pirbright Institute
Year(s) Of Engagement Activity 2016,2017
 
Description University of Surrey School of Veterinary Medicine Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Presented research activity from the Pirbright Institute
Year(s) Of Engagement Activity 2018
 
Description V Nair invited to talk on 'Cell type- and disease stage-specific interactions of Marek's disease virus & the host' at the International Symposium on Marek's disease and avain herpesiviruses at Yangzhou, China during 29th July to 2nd Aug 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Most recent updates in the field of avian herpesvirus research was discussed and active engagements for future collaborations discussed
Year(s) Of Engagement Activity 2018
 
Description V Nair was invited to talk a the Poultry Health Workshop and Conference on 19th-20th Nov, 2018 in Kathmandu, Nepal 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact more than 100 stakeholders from different sectors of the Poultry industry and academia attended the meeting. There was engagement from the different sectors for initiating a combined approach for finding solutions
Year(s) Of Engagement Activity 2018
URL http://www.cmdn.org.
 
Description Vet School Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact University of Surrey School of Veterinary Medicine Open Day - introduced The Pirbright Institute and the research we conduct to prospective students and parents.
Year(s) Of Engagement Activity 2018
 
Description Vet Times 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Information on research activity of Avian Immunology group at the Pirbright Institute
Year(s) Of Engagement Activity 2018
 
Description Veterinary Research Club 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Presentation of the data generated and its importance in understanding immune responses to vaccination and natural infections. About 50 veterinarians and researchers working in veterinary sciences attended the one-day meeting, which initiated discussions about the possibility of generating new vaccines against BRSV.
Year(s) Of Engagement Activity 2017
 
Description Video on African swine fever clinical signs for APHA 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Video prepared to inform farmers and vets about African swine fever virus clinical signs
Year(s) Of Engagement Activity 2018
URL https://www.youtube.com/watch?v=z4kSR-wMO4M&feature=youtu.be
 
Description Virtual video tour of the animal facilities in Pirbright 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Contributed to public understanding of animal research by being interviewed on the virtual video tour of the animal facilities in Pirbright - one of only four such initiatives in the UK.
Year(s) Of Engagement Activity 2017
 
Description Winston Churchill Careers Fair. Winston Churchill School, Hermitage Road, St Johns, Woking, Surrey, GU21 8TL 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact AS attended and presented at this event to stimulate increased interest in science and research.
Year(s) Of Engagement Activity 2018
 
Description a talk at the Recently Independent Virology Investigators meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talks outlining my current research and future plans.
Year(s) Of Engagement Activity 2015,2016,2017
 
Description organised workshops at Microbiology Society annual conferences 2014 - 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I co-organised virology division workshops on viral evolution and diversity and RNA viruses with colleagues from the virology division. The workshops were held at the annual Microbiology Society conferences in spring each year. Organisation involved selecting abstracts for oral or poster presentation, ordering the presentations and chairing the sessions.
Year(s) Of Engagement Activity 2015,2016,2017,2018