Molecular and Systems Virology

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

The Pirbright Institute provides the UK with capacity to predict, detect, understand and respond to incursions of viral pathogens of livestock and viruses that spread from animals to humans. Pirbright represents a hub of world class facilities and expertise for the study of exotic and endemic viral diseases in the natural host, therefore enabling our research to be directly translated into future control measures.
This project will identify virus-host interactions that influence the outcome of infection and that could inform the future development of gene edited or genetically modified animals that are resistant to infection or have reduced ability for onwards transmission. It will provide novel fundamental knowledge of the molecular mechanisms of viral replication and viral subversion of innate immunity, leading to novel, safe strategies for virus attenuation. These findings will be applied to the rational design of novel or improved vaccines or other control strategies. The approaches used to achieve these goals will include classical techniques from virology and immunology alongside confocal and super-resolution fluorescent microscopy and electron tomography, transcriptomics, proteomics, Y-2-H and systematic genome-wide RNAi screening approaches. Anticipated outcomes of this project are: Improved fundamental knowledge of the virus-cell interactions that influence the outcome of infection in vitro; high-resolution structures of viruses and viral proteins to inform vaccine design; identification of key cellular innate pathways which are disrupted by virus infection; novel, safe, rational strategies for virus attenuation; identification of novel targets for antivirals; provision of novel in vitro information to support the development of in vivo pathogenesis models.

Organisations

Publications

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Charleston B (2018) Recent advances in veterinary applications of structural vaccinology. in Current opinion in virology

 
Description The Molecular and Systems Virology project is part of the BBSRC Institute Strategic Programme "Understanding and preventing viral diseases". There are three objectives and key findings from the first two years of this project are described below.

Objective 1: Characterising viral and cellular determinants of tropism and host specificity.
For infectious bronchitis virus (IBV) the S2' cleavage site within the spike glycoprotein was found to be responsible for the extended cell culture tropism of the Beaudette strain of IBV. This may allow the engineering of vaccine strains which can be propagated in cell culture, reducing the number of eggs required for IBV vaccine production. For avian leukosis virus (ALV), gene editing of specific cellular receptors was found to render chicken cell lines resistant to virus infection, with implications for future strategies for gene editing for disease resistant chickens.

Objective 2: Understanding the molecular mechanisms of viral replication.
We have demonstrated that blue-tongue virus (BTV) of a low-passage insect-cell isolate enters bovine endothelial cells by a non-specific entry mechanism called macropinocytosis. This is the first time BTV entry has been studied using a virus strain not adapted to mammalian cell-culture and using cells of relevant type.
Electron tomography methods have been developed and used to demonstrate the role of IBV nsp4 in the membrane re-arrangements involved in coronavirus replication. Electron microscopy has also been used to show that the temperature sensitive phenotype of an attenuated IBV strain is associated with an impairment in viral RNA synthesis and malformation of viral replication organelles, providing a new approach to study the composition and function of coronavirus replication organelles.
For foot-and-mouth disease virus (FMDV) new understanding has been gained for several aspects of virus assembly and capsid stability. We have shown that capsid precursor processing and assembly of capsid pentamers requires cellular chaperones, that packaging of the genome into the capsid involves packaging signals in the RNA and that the small internal capsid protein VP4 is involved in virus stability. We have also shown that a novel inhibitor of the host enzyme N-myristoyltransferase can block replication of multiple picornaviruses by blocking the myristolyation of capsid precursors and thereby preventing the assembly of mature capsids.

Objective 3: Defining how viruses manipulate the cellular response to infection.
For influenza viruses, we have identified that a small accessory protein, PB1-F2, is able to bind to the chicken homologues of MAVS and IKKß to disrupt interferon and pro-inflammatory signalling. The sequence of PB1-F2 influences protein cellular localisation and stability which may fine tune the degree of antagonism of these signalling pathways.
For African swine fever virus (ASFV), new virus-host interactions have been identified which control multiple aspects of the cellular response to infection: Viral protein A179L was found to have potent anti-apoptotic activity by uniquely binding to all major pro-apoptotic host Bcl-2 family proteins. We have also identified the step at which ASFV controls the highly conserved cellular autophagy pathway and have discovered a viral protein that may be involved in manipulating this cellular response. Further ongoing studies have identified additional multiple viral proteins which inhibit activation of type I interferon and NF-kB activated responses. All of these findings will inform the design of next generation ASF vaccines.
Exploitation Route Too early to say (the award is still active)
Sectors Agriculture, Food and Drink,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology

 
Description Deletion of genes that inhibit innate immune responses has been shown to attenuate virulent ASFV isolates and provide a route for constructing rationally attenuated live vaccine candidates. Of the genes we have studied deletions of DP148R and members of the MGF 360 and 505 have been included in a patent application as candidate live attenuated vaccines. We expect other genes we have identified to provide targets for rational attenuation of ASFV to produce candidate vaccines. Research findings have been presented to vaccine producers as a viable alternative to producing certain strains of foot-and-mouth vaccines with improved stability.
First Year Of Impact 2018
Sector Agriculture, Food and Drink,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology
Impact Types Societal,Economic

 
Description Coalition for Epidemic Preparedness Innovations Nipah Taskforce Member
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Contributing to the chapter on Marek's disease for the OIE Terrestrial Manual
Geographic Reach Multiple continents/international 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
Impact The contribution to the Terrestrial Manual on the recent advances in the diagnosis and control of Marek's disease will impact on improved and better control of the disease to reduce losses and improved food security
URL http://www.oie.int/
 
Description Contribution to Annual Report of the Chief Medical Officer UK (2017), Genomics Chapter 9.
Geographic Reach Europe 
Policy Influence Type Gave evidence to a government review
 
Description Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals'
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Development and Evaluation of PPR DIVA vaccines
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in other policy documents
Impact Peste des petits ruminants (PPR) is a highly contagious disease, severely affects small ruminants in almost 70 countries in Africa, the Middle East and parts of Asia. It causes USD 1.5 to 2 billion in losses each year in regions that are home to over 80% of the world's sheep and goats and to more than 330 million of the world's poorest people, many of whom depend on them for their livelihoods. The estimated current expenditure on PPR vaccination ranges between USD 270 and 380 million. The annual impact of PPR alone may be valued at between USD 1.45 and 2.1 billion per year. Approximately a third of the global financial burden of PPR is borne by Africa, with a further quarter borne by South Asia. This burden will be removed with the successful eradication of PPR. The undiscounted costs for a fifteen-year Global control Strategy of FAO and OIE are between USD 7.6 and 9.1 billion, with the first five years costing between USD 2.5 and 3.1 billion. (http://www.fao.org/3/a-i4460e.pdf). PPR is currently controlled by vaccination using mainly two live attenuated PPRV vaccines (Nigeria 75/1 and Sungri 96). However, the current vaccines and serological tests are unable to enable Differentiation between naturally Infected and Vaccinated Animals (DIVA). This factor precludes meaningful assessment of vaccine coverage and epidemiological surveillance based on serology, in turn reducing the efficiency of control programmes. Therefore, it is almost impossible to assess the quality and efficacy of existing PPR vaccines without knowing whether positive animals are vaccinated or naturally infected. Unlike rinderpest, where cattle and buffalo were primary hosts, in PPR, new crops (about 30-40%) of lambs and kids are produced every year and are the most susceptible population to bring back new PPR outbreaks6&7. Therefore, it is likely that the cycle of vaccinations and PPR outbreaks will continue until and unless we reach the stage of 80-90% herd immunity. Therefore, development of a marker vaccine and associated DIVA diagnostics will enable the assessment of vaccine efficacy which is essential for any successful vaccination campaign. https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-peste-des-petits-ruminant The availability of a recombinant PPRV vaccine with a proven functionality is a prerequisite for the development of novel vaccines that may enable the development of DIVA tools for PPRV diagnostics. In the DBT-BBSRC FADH BB/L004801/1 grant we have rescued Sungri 96 and Nigeria 75/1 vaccine strains. Both the vaccine strains were rescued from respective synthetic c-DNA clones with mutations in the variable part of C terminus of the nucleocapsid (N) gene similar to Dolphin Morbillivirus (DMV) to enable negative marker DIVA vaccines. These two DIVA vaccines along with parental vaccines have been recently tested in a pilot studies in goats. Both the DIVA and parent vaccines provided safety, stability and protection for vaccinated goats whereas the control animals were clinically infected. Patent applications have been made to protect these DIVA vaccines. Agreements are being done with vaccine industries for the licensing and commercialisation.
URL https://www.pirbright.ac.uk/press-releases/2018/09/pirbright-collaboration-provides-tools-peste-des-...
 
Description IUCN Technical Subgroup on Synthetic Biology and Biodiversity Conservation
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Lumpy Skin Disease Control Strategy for Great Britain
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Netherlands Commission on Genetic Modification (COGEM) Project "Gene drives, inventory of previous research and experience"
Geographic Reach Europe 
Policy Influence Type Gave evidence to a government review
 
Description Organised seminar series on Sequencing, Virus Evolution and Molecular Biology at Pirbright (with Luca Ferretti and Graham Freimanis)
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
 
Description PPR Global Research and Expertise Network (PPR GREN) membership (OIE/FAO)
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Participation in the Management of the Oxford DTP
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact As part of the Management committee of the Oxford-Pirbright-Oxford Brookes-Diamond doctorate Training Partnership, contributed to the success of the DTP in meeting the objectives, recruitment and review
URL http://www.biodtp.ox.ac.uk/
 
Description Prevention and Control of LSD
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
Impact I have participated as an expert advisor on committees and panels focused on the control of lumpy skin disease (LSD) in Europe. This has contributed to the formulation of an effective control strategy, focused on the use of live-attenuated vaccines, in south-eastern Europe. The effective control strategy has prevented LSD from entering central Europe. The economic benefit of restricting LSD spread to central Europe is the major impact. This is difficult to estimate but in the millions of Euros.
 
Description RCUK Engagement workshop, Cosener's House, 20-21 April 2017
Geographic Reach National 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Taught course on introductory bioinformatics to PhD students at Pirbright
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Training MSc students
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
Impact Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.
 
Description A One Health approach to pan-valent morbillivirus vaccines
Amount £773,237 (GBP)
Funding ID BB/R019843/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2018 
End 03/2021
 
Description BBSRC - responsive mode
Amount £187,370 (GBP)
Funding ID BB/S007911/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2019 
End 03/2022
 
Description BBSRC Responsive Mode IPA
Amount £302,086 (GBP)
Funding ID BB/R002606/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 06/2019
 
Description Biotechnology and Biological Sciences Research Council (BBSRC) and University of Oxford joint Doctoral Training Project
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2018 
End 08/2022
 
Description British Egg Marketing Board PhD studentship
Amount £76,000 (GBP)
Organisation British Egg Marketing Board 
Sector Private
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description DARPA PREEMPT
Amount $2,700,000 (USD)
Organisation Defense Advanced Research Projects Agency (DARPA) 
Sector Public
Country United States
Start 10/2018 
End 03/2022
 
Description Further development of infectious bronchitis virus vaccines adapted for production in cell culture
Amount £262,701 (GBP)
Funding ID BB/R019576/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 03/2020
 
Description Genomia Project Grant
Amount £174,052 (GBP)
Organisation Genomia fund 
Sector Charity/Non Profit
Country United Kingdom
Start 02/2019 
End 01/2020
 
Description H2020 work programme topic 9 Food security, sustainable agriculture and forestry, marine and maritime and inland water research and the bioeconomy, work topic SFS-10-2017 Research and approaches for emerging diseases in plants and terrestrial livestock
Amount € 5,986,250 (EUR)
Organisation European Commission H2020 
Sector Public
Country Belgium
Start 05/2018 
End 04/2023
 
Description LVIF
Amount $1,500,000 (CAD)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 04/2018 
End 09/2020
 
Description NC3Rs Research Project Grant
Amount £436,339 (GBP)
Funding ID NC/R001138/1 
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Private
Country United Kingdom
Start 12/2017 
End 11/2019
 
Description Pathfinder BB/R012431/1 Grant holder: Dr Mark Fife. Grant title: chIFITM knockdown/knockout technology as a platform technology for increased vaccine yields in SPF Eggs.
Amount £12,500 (GBP)
Funding ID BB/R012431/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2017 
End 02/2018
 
Description Pirbright Institute PhD studentship
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2018 
End 08/2022
 
Description Taiwan Partnering Award
Amount £40,700 (GBP)
Funding ID BB/S020624/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start  
 
Title A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR 
Description Quantitative real-time PCR assays are widely used for the quantification of mRNA within avian experimental samples. Multiple stably-expressed reference genes can be used to control random technical variation between samples. It is necessary to select reference genes with the lowest variation in representative samples. The candidate reference gene assays must be reliable. In particular, they should have high amplification specificity and efficiency, and not produce signals from contaminating DNA. Whilst recent research papers identify specific genes that are stable in particular tissues and experimental treatments, here we describe a panel of ten avian gene primer and probe sets that can be used to identify suitable reference genes in many experimental contexts. The panel was tested with TaqMan and SYBR Green systems in two experimental scenarios, a tissue collection, and virus infection of cultured fibroblasts. GeNorm and NormFinder algorithms were able to select appropriate reference gene sets in each case. We show the effects of using the selected genes on the detection of statistically significant differences in expression. The results are compared with those obtained using 28s ribosomal RNA, the present most widely accepted reference gene in chicken work, identifying circumstances where the use of this gene might provide misleading results. Widely used methods for eliminating DNA contamination of RNA reduced, but did not completely remove, detectable DNA. We therefore attached special importance to testing each qPCR assay for absence of signal using DNA template. 
Type Of Material Technology assay or reagent 
Year Produced 2016 
Provided To Others? Yes  
Impact The assays and analyses developed here provide a useful resource for selecting reference genes for investigations of avian biology. 
 
Title Comprehensive analysis of genetic variants across immune loci in indigenous breeds. 
Description The interferon-induced transmembrane (IFITM) protein family comprises a class of restriction factors widely characterised in humans for their potent antiviral activity. Their biological activity is well documented in several animal species, but their genetic variation and biological mechanism is less well understood, particularly in avian species. Here we report the complete sequence of the domestic chicken Gallus gallus IFITM locus from a wide variety of chicken breeds to examine the detailed pattern of genetic variation of the locus on chromosome 5, including the flanking genes ATHL1 and B4GALNT4. We have generated chIFITM sequences from commercial breeds (supermarket-derived chicken breasts), ancient bone samples, indigenous chickens from Nigeria (Nsukka) and Ethiopia, European breeds and inbred chicken lines from The Pirbright Institute, totalling of 211 chickens. Through mapping of genetic variants to the latest chIFITM consensus sequence our data reveal that the chIFITM locus does not show structural variation in the locus across the populations analysed, despite spanning diverse breeds from different geographic locations. However, single nucleotide variants (SNVs) in functionally important regions of the proteins within certain groups of chickens were detected, in particular the European breeds and indigenous birds from Ethiopia and Nigeria. In addition, we also found that two out of four SNVs located in the chIFITM1 (Ser36 and Arg77) and chIFITM3 (Val103) proteins were simultaneously under positive selection. Together these data suggest that IFITM genetic variation may contribute to the capacities of different chicken populations to resist viral infection. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2019 
Provided To Others? Yes  
Impact This paper is currently in press in. BMC Genomics. 
 
Title Deep sequencing to identify RNA packaging signals 
Description A novel and simple approach to identify predicted RNA secondary structures involved in genome packaging in positive sense RNA viruses (e.g. the picornavirus foot-and-mouth disease virus [FMDV]). By interrogating deep sequencing data generated from both packaged and unpackaged populations of RNA, we have determined multiple regions of the genome with constrained variation in the packaged population. Predicted secondary structures of these regions revealed stem-loops with conservation of structure and a common motif at the loop. Disruption of these features resulted in attenuation of virus growth in cell culture due to a reduction in assembly of mature virions. This study provides evidence for the involvement of predicted RNA structures in picornavirus packaging and offers a readily transferable methodology for identifying packaging requirements in many other viruses. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact Impact so far is fundamental knowledge but with several potential avenues for future impact in for example improved vaccines. 
 
Title Development of assays for quantifying fusion inhibitory antibodies in sera (mFIT assay) 
Description We have developed a micro-fusion inhibition test (mFIT) assay to identify and quantify antibody responses (to vaccines etc.) which are capable of inhibiting viral induced cell-cell fusion. This test has been developed for various negative strand viruses. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact This assay has allowed us to provide a more detailed examination of neutralising antibody responses to vaccines and when published will be disseminated to the field. 
 
Title IBDV Reverse Genetics System 
Description We have developed a reverse genetics system for IBDV. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity. 
 
Title Identified the best sampling material for the PPR virus genome detection 
Description Quantifying the DNA from all the body fluids of PPR virus infected goats we demonstrated that non-invasive nasal samples are the best material for the genome detection as virus appears as early as 2 days post infection before any clinical signs appear and the virus detection linger for this sample type than any other sample. 
Type Of Material Biological samples 
Year Produced 2019 
Provided To Others? Yes  
Impact As we have shown the best sample type for the detection of PPR virus, we have not to do many sample analysis which save the money and effort during the eradication programme. 
 
Title Optimisation of chicken kidney cell culture preparation 
Description We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus. 
Type Of Material Cell line 
Year Produced 2017 
Provided To Others? No  
Impact This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information. 
 
Title Optimisation of preparation of TOCs from adult chickens 
Description Tracheal organ cultures (TOCs) are prepared from chicken embryos and used to assess growth of avian viruses that are not able to be propagated in cell culture. We have optimised the method of TOCs preparation from 3-week old chickens and are using these to assess viral genetic stability and phenotype in organ cultures that are a similar environment to the site of replication in whole chickens. 
Type Of Material Cell line 
Year Produced 2018 
Provided To Others? No  
Impact We hope that this technique will reduce the number of chickens required for in vivo experiments, in line with the 3Rs, and plan to publish the method in due course. 
 
Title Stable chicken cell lines overexpressing chicken IFITM1,2 and 3 
Description In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV. 
 
Title Use of reverse genetics to develop PPR DIVA vaccines 
Description Reverse genetics technique has been established for PPR virus in our laboratory. As PPR vaccine is a live attenuated virus, it is not possible to differentiate between vaccinated and infected animals (DIVA) in existing antibody assays. However using reverse genetics technique we have manipulated/mutated residues in the full-length cDNA of virus and rescued the live attenuated vaccine strain which worked as a DIVA vaccine. Using this technique a GFP ( Green fluorescent protein) has been introduced into the virulent PPR virus that helped to follow the virus in the infected goats. Similarly using this technique we have modified the existing live attenuated viruses ( Nigeria 75/1) and Sungri 96/1) in to recombinant marker vaccines that enables to differentiate between infection and vaccination ( DIVA). So we have demonstrated that reverse genetics tool can be used to study the pathogenesis and to develop the marker vaccines. This technique can be adapted for other negative strand viruses to design the DIVA vaccines. 
Type Of Material Technology assay or reagent 
Year Produced 2015 
Provided To Others? Yes  
Impact PPR DIVA vaccine developed that can differentiate between vaccinated and infected animals. This will be helpful to know the efficacy of vaccine by knowing the presence antibodies in animal is due to vaccination or infection. This will reduce the eradication time and will facilitate to declare freedom of diseases as soon as Possible without long waiting period. 
 
Title chimeric IBDVs 
Description Andrew Broadbent has engineered chimeric IBDVs expressing individual genes from a very virulent strain in the backbone of an attenuated strain. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact These chimeric strains can be used to define viral determinants of virulence. 
 
Title replication competent recombinant split-GFP infectious bursal disease virus 
Description We have generated a replication competent recombinant split-GFP infectious bursal disease virus (IBDV) where a small GFP11 molecule is tagged to the virus polymerase (VP1) to make IBDV-VP1-GFP11. Infected cells that express the GFP1-10 molecule fluoresce green when the GFP11 tag complements the GFP1-10. The IBDV-VP1-GFP11 colocalises with VP3 and dsRNA and therefore is likely to be present at the sites of virus replication. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact This tool can be used to study IBDV replication complex development in infected cells. 
 
Title tetracysteine-tagged IBDV 
Description Andrew Broadbent has engineered a tetracysteine (TC)-tagged IBDV that can be used in live cell imaging and co-infection studies with the split-GFP IBDV. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact This tool, combined with the split-GFP IBDV, will enable us to study co-infection, super-infection exclusion, and the intracellular requirements for viral reassortment. 
 
Title Analysis of paramyxovirus replication using Incucyte real-time imager 
Description We have optimised a data analysis pipeline for the Incucyte real-time imager to allow us to track viral replication in a high-throughput fashion (up to 6 x 96-well plates). This is based on simultaneous analysis of phase, as well as green and red fluorescence. 
Type Of Material Data analysis technique 
Year Produced 2018 
Provided To Others? No  
Impact Only preliminary data to date. 
 
Title Genome browser for MDV infected chicken cells 
Description The database integrates and makes available the results of the bioinformatics analysis of several experiments involving high-throughput sequencing of chicken cells infected by several viruses (mainly Marek's Disease Virus) generated within the Avian Viral Disease group at the Pirbright Institute. 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? Yes  
Impact The genome browser allows the researchers to meaningfully mine and integrate the large amount of data produced by their experiments, guiding subsequent experimental hypothesis-driven validation in the lab. 
 
Title Method to detect the expression of repetitive genes 
Description Reconstructing expression levels of genes having a high repeat content is difficult, due to the impossibility of assigning RNA-sequencing that have multiple mappings to their correct originating location. In collaboration with the Immunogenetics group, the Integrative Biology and Bioinformatics groups are developing a data analysis method to circumvent the problem.The method has already been shown to be effective on some KIR genes in cattle. An essential component of the immune system, their family is rich in repeated members and repetitive regions, making the technique essential if one wants to study them. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact Being able to resolve the expression of repetitive genes, which are plentiful in most mammalian genomes, would be essential to the high-precision study of many important gene families, including most of the genes of the immune system. The technique has already proven itself effective on a real-life problem, and holds the potential to be extended to many more biological systems 
 
Title Viral assembly pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust viral assemblies out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, assembling of viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; uneven coverage due to low-quality material or the genomic material of the virus being RNA; the viral nucleic acid being almost lost in the background of nucleic acid of the host. Our pipeline overcomes most of those problems, and is even able to detect different viral strains being present together in the same sample. It can also be used on metagenomic environmental samples. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Title Viral variant calling pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust variant calling for viral sequences out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, calling variant for viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; very high genome coverage, which slows down most existing variant callers; the presence of a potentially very high number of different haplotypes for RNA viruses, which is an unusual scenario in variant calling. Our fast and sensitive Bayesian pipeline overcomes most of those problems. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Description Andrew Davidson - Proteome of viral replication organelles 
Organisation University of Bristol
Department Avon Longitudinal Study of Parents and Children (ALSPAC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution Andrew has provided assistance with experimental planning and drafting of a grant application. He has also provided protocols to allow preliminary data to be generated. Once protocols have been established, Andrew will facilitate mass spectrometry data generation and will analyse the resulting data.
Impact No outcomes to date, projects being initiated and funding applications in progress.
Start Year 2018
 
Description Annika Posautz; University of Veterinary Medicine Vienna. Morbillivirus host-diversity analysis. 
Organisation University of Veterinary Medicine Vienna
Country Austria 
Sector Academic/University 
PI Contribution Sharing of research data regarding morbillivirus host-range and technical support to the project.
Collaborator Contribution Provision of tissue samples from potential morbillivirus hosts, e.g. carnivores.
Impact No outcomes as yet.
Start Year 2018
 
Description Ben Neuman - Study of IBV induced membrane rearrangements 
Organisation Texas A&M University–Texarkana
PI Contribution My team provide samples for electron microscopy analysis. In addition, we provide expertise in molecular virology and cell biology and experience of membrane rearrangements induced by infectious bronchitis virus. I contribute intellectual input in data analysis and project development.
Collaborator Contribution Ben Neuman and his team perform electron microscopy sample processing and imaging as well as experience in data processing. Ben Neuman also contributes intellectual input in data analysis and project development.
Impact Maier et al. Extensive coronavirus induced membrane rearrangements are not a determinant of pathogenicity. Sci. Rep. 6: 27126.
Start Year 2014
 
Description Ben Neuman - Study of IBV induced membrane rearrangements 
Organisation University of Reading
Country United Kingdom 
Sector Academic/University 
PI Contribution My team provide samples for electron microscopy analysis. In addition, we provide expertise in molecular virology and cell biology and experience of membrane rearrangements induced by infectious bronchitis virus. I contribute intellectual input in data analysis and project development.
Collaborator Contribution Ben Neuman and his team perform electron microscopy sample processing and imaging as well as experience in data processing. Ben Neuman also contributes intellectual input in data analysis and project development.
Impact Maier et al. Extensive coronavirus induced membrane rearrangements are not a determinant of pathogenicity. Sci. Rep. 6: 27126.
Start Year 2014
 
Description Collaboration with Bauchi State Veterinary Services, Nigeria 
Organisation Bauchi State University
PI Contribution We have provided expertise on sheeppox, goatpox and lumpy skin disease virus diagnosis and epidemiology
Collaborator Contribution Our collaborators in Bauchi State have provided virus samples and epidemiological data
Impact Preliminary data was used to support successful funding via a BBSRC Impact Accelerator Award, and a Pathways to Impact project in BBSRC project BB/R008833/1 "Development of rationally designed live-attenuated lumpy skin disease vaccines"
Start Year 2016
 
Description Collaboration with State Central Veterinary Laboratory, Mongolia 
Organisation State Central Veterinary Laboratory
PI Contribution We provided tools, techniques and expertise on sheeppox and goatpox
Collaborator Contribution Our colleagues provided data and field samples
Impact A workshop was held in Pirbright in March 2017, and a return workshop in Ulaanbaatar in July 2017. Preliminary data supported successful grant applications for an Impact Accelerator Award, and a Pathways to Impact project in BB/R008833/1 "Development of rationally designed live-attenuated lumpy skin disease vaccines".
Start Year 2016
 
Description DTU 
Organisation Technical University of Denmark
Country Denmark 
Sector Academic/University 
PI Contribution Joint PhD studentship
Collaborator Contribution Joint PhD studentship
Impact Joint PhD studentship in progress
Start Year 2016
 
Description David Matthews - Proteomics informed by transcriptomics 
Organisation University of Bristol
Department Proteomics Facility
Country United Kingdom 
Sector Academic/University 
PI Contribution Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution David has assisted in project design and drafting of a funding application to BBSRC. We are in the process of generating transcriptomics data, which David will process to provide an expressed protein database for peptide identification following mass spectrometry.
Impact No outcomes to date, a funding application has been submitted to BBSRC.
Start Year 2018
 
Description Edinburgh 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution Co-INvestigator on BBSRC sLoLa award
Collaborator Contribution Co-INvestigator on BBSRC sLoLa award
Impact BBSRC sLoLa award ongoing
Start Year 2012
 
Description Factor H and influenza virus 
Organisation Brunel University London
Department Brunel Arts
Country United Kingdom 
Sector Academic/University 
PI Contribution Our research team are hosting a PhD student from Brunel University to facilitate the investigation of any interaction between influenza virus and complement factor H. We are providing reagents, expertise in handling influenza virus and advice on the scientific hypothesis of the research programme.
Collaborator Contribution Brunel university initiated the project and provided reagents including antibodies and methods for purification human co-factor H protein. They also provide funding for this PhD studentship.
Impact No outcomes as yet
Start Year 2018
 
Description Genetic analysis orbiviruses 
Organisation University of Surrey
Department Faculty of Health and Medical Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Virus sequences provided for analysis. Meeting and discussion for analysis by virologist employed on this project and biomathematican at the University of Surrey
Collaborator Contribution Analysis of data, training of the virologist and computational and mathematical methodology, discussion about analysis strategies
Impact The collaboration is multi-disciplinary - between mathematical modelling and virology. Data analysis is still ongoing but is envisaged as preliminary data for future funding applications
Start Year 2017
 
Description Horizon Discovery Ltd Cambridge Research Park, United Kingdom 
Organisation Horizon Discovery Group plc
PI Contribution Verify IFITM knock-out cell by qPCR and immuno-blotting. Verification of further knock-out cell lines by DNA sequencing, immuno-blotting and qPCR Cell infection with Avian Influenza Viruses Assess the viral titre of cells infected with Influenza Viruses in K/O and wildtype cells. Quantitative analysis of viral infection in IFITM K/O cells Demonstrate increased AIV viral titres at lab scale and estimation of what this may equate to with respect to vaccine dose yields. Analysis of IFITM K/O cell lines permissivity to a range of additional animal viruses (Relevant to the LVIF). Milestone 7: Demonstrate permissivity increased and viral titres for a range of LVIF relevant viruses in the edited cell lines.
Collaborator Contribution Develop a CRISPR/cas9 gene editing system to specifically target the chIFITM locus in avian cell culture
Impact Grant funding
Start Year 2017
 
Description ILRI Research Collaboration (Kenya, March 2017) 
Organisation International Livestock Research Institute (ILRI)
Country Kenya 
Sector Charity/Non Profit 
PI Contribution Exchange of ideas and samples for indigenous breeds of livestock
Collaborator Contribution Contributed samples for analysis.
Impact Analysis of indigenous breed genomic sequences for Immune function genes.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Imperial 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Joint supervision of PhD student
Collaborator Contribution Joint supervision of PhD student
Impact One successful completed PhD student. Papers published or in preparation. Another PhD student in progress.
Start Year 2010
 
Description Industrial partnership-Aviagen 2018 
Organisation Aviagen Group
Country United States 
Sector Private 
PI Contribution We have established a significant industrial partnership with Aviagen group Ltd. The nature of this work remains confidential.
Collaborator Contribution The nature of this work remains confidential.
Impact The nature of this work remains confidential.
Start Year 2018
 
Description Influenza and co-factor H protein 
Organisation Leibniz Association
Department Leibniz Institute for Natural Product Research and Infection Biology
Country Germany 
Sector Public 
PI Contribution We are screening influenza virus interaction with co-factor H proteins to determine the interaction interface.
Collaborator Contribution Christine Skerka has provided fragment factor H peptides to facilitate our mapping studies of the interaction surface with influenza viruses.
Impact No outcomes as yet
Start Year 2019
 
Description Interferon stimulated genes 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Hosted meetings and carried out preliminary experiments
Collaborator Contribution Contributed reagents and expertise
Impact Preliminary data was used to support an MSc project. This has since led to a publication and a PhD project.
Start Year 2016
 
Description JS - Production of monoclonal antibodies that recognise FMDV VP4 from mouse spleens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution VLPs displaying the N-terminal 15 amino acids of FMDV were designed and produced at The Pirbright Institute and then mice were immunised with them. The spleens of the immunised mice were harvested and splenocytes stored. The response to the VP4 sequence was checked by ELISA and the mouse with the best response was selected to send the splenocytes for monoclonal production.
Collaborator Contribution The collaborators took the splenocytes obtained from the mouse experiments and carried out the fusions. They then screened the supernatants for positive wells that detected the VP4 sequence.
Impact Positive wells have been identified indicating some monoclonals that are specific for VP4 have been generated. These will be further screened for ability to recognise virus and to neutralise infection.
Start Year 2017
 
Description Martin Ludlow - University of Veterinary Medicine, Hanover. Morbillivirus collaboration. 
Organisation University of Veterinary Medicine Hanover
PI Contribution Provision of technical information and advice about the host-range of morbilliviruses.
Collaborator Contribution Provision of reagents to support our research project, e.g. plasmid clones of viral attachment proteins and receptors.
Impact No outcomes as yet, only preliminary data.
Start Year 2018
 
Description Membrane protein interactions 
Organisation University of Liverpool
Department School of Veterinary Science Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Samples from cells transduced with ASFV membrane proteins have been prepared.
Collaborator Contribution Samples have been analysed for proteins interacting with ASFV membrane proteins by mass spectrometry.
Impact Collaboration still in progress
Start Year 2017
 
Description Miguel Ángel Muñoz Alía - Mayo clinic, USA. Measles population diversity. 
Organisation Mayo Clinic
Country United States 
Sector Charity/Non Profit 
PI Contribution Technical support and sharing of research data on the host-range of morbilliviruses.
Collaborator Contribution Provision of plasmid clones representing the broad genetic diversity of measles attachment and fusion proteins. Technical support.
Impact No outcomes as yet.
Start Year 2018
 
Description Montse Barcena - Studying the role of coronavirus membrane rearrangements 
Organisation Leiden University Medical Center
Country Netherlands 
Sector Multiple 
PI Contribution My team has provided reagents and biological samples for the study of the role of IBV induced membrane rearrangements. In addition we have provided expertise and knowledge for the set up of protocols and assisted in data analysis.
Collaborator Contribution M Barcena has provided extensive experience in electron microscopy including use of specialised protocols. She has provided expertise in data analysis and protocol development.
Impact Several presentation of data have been made at international conferences.
Start Year 2014
 
Description Nicolas Locker - Investigation of translation regulation by infectious bronchitis virus 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution My team provide experience in molecular virology and cellular interactions of infectious bronchitis virus including previous experience of IBV accessory proteins regulating cellular translation. A PhD student works jointly between our labs.
Collaborator Contribution Dr Locker's team provide expertise in viral regulation of cellular translation and stress granule signalling. Dr Locker is also providing protocols, advice and training for techniques including ribopuromycylation and ribosome profiling. A PhD studentworks jointly between our labs.
Impact PhD student funding (studentship commenced Oct 2017) - Control of host cell translation by infectious bronchitis virus
Start Year 2016
 
Description Oxford - Strubi 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on virus structural biology and joint PhD studentship
Collaborator Contribution Collaboration on virus structural biology and joint PhD studentship
Impact Collaboration on virus structural biology has produced a number of novel virus structures and new understanding of virus entry and packaging
Start Year 2008
 
Description Partnering with National Taiwan University 
Organisation National Taiwan University
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity".
Collaborator Contribution The partners conceived the project and got in contact about submitting an application for funding together.
Impact An application has been made for a BBSRC-Taiwan Partnering Award in November 2018.
Start Year 2018
 
Description Paul Digard - The Roslin Insititute 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We generated constructs that express PB1-F2 from a variety of avian influenza strains in eukaryotic expression plasmids. We generated Chimera PB1-F2 expression plasmids where the C-terminus of the PB1-F2 are switched between strains. Using these plasmids we have determined the localization and antagonist activity toward the IFN beta signaling pathway and NF-kb signaling pathway in human and chicken cells. We have therefore provided reagents and intellectual input to the collaboration.
Collaborator Contribution Our collaborators have used these plasmids in there well defined stability assays to determine sequence specific effects on this characteristic. Intellectual input and expertise in a particular assay were made by these collaborators.
Impact We have successfully published some of this work; "The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells." doi: 10.1099/jgv.0.001220. We have an ongoing joint PhD studentship in this area. This collaboration remains current and active.
Start Year 2017
 
Description Paul Verkade - Electron tomography and CLEM 
Organisation University of Bristol
Department Avon Longitudinal Study of Parents and Children (ALSPAC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Nicole Doyle has performed electron tomography to analyse the three dimensional structure of membrane rearrangements induced by the expression of IBV non-structural proteins 4 or 3 and 4 in the absence of other viral components. Sample set up was performed at Pirbright and analysis was subsequently performed at University of Bristol followed by additional analysis at Pirbright.
Collaborator Contribution Paul has assisted with electron tomography data generation providing access to electron microscopes in Bristol as well as training for Nicole Doyle in performing experiments and analysing and modelling data. He is also advising Nicole while establishing correlative light electron microscopy at Pirbright.
Impact doi: 10.3390/v10090477
Start Year 2018
 
Description Pseudoparticles of influenza for interaction studies with co-factor H 
Organisation University of Kent
Department Medway School of Pharmacy
Country United Kingdom 
Sector Hospitals 
PI Contribution We have screened the interaction between influenza pseudotype particles that only contain the surface HA protein from influenza viruses for interaction with human co-factor H protein. We have screen pseudotype particles of multiple influenza subtypes.
Collaborator Contribution Nigel Temperton of the viral Pseudotype Unit at the School of Pharmacy provided influenza virus pseudotype particles of multiple subtypes to allow us to screen for interaction with co-factor H protein.
Impact No outcomes yet
Start Year 2018
 
Description Re-annotation of Marek's Disease Virus 
Organisation U.S. Department of Agriculture USDA
Department Beltsville Agricultural Research Center
Country United States 
Sector Academic/University 
PI Contribution We are using bioinformatics analysis of high-throughput sequencing data in order to provide a better annotation of the genome of Marek's Disease Virus
Collaborator Contribution Our partners are sharing their extensive expertise of the genomics of MDV, and performing experimental validations
Impact No output yet
Start Year 2015
 
Description Research Collaboration with Nigeria (23rd - 28th January 2017) 
Organisation University of Nigeria
Country Nigeria 
Sector Academic/University 
PI Contribution Analysis of rare breed indigenous chicken in Nigeria.
Collaborator Contribution Helped with sampling and background information of the breed.
Impact Analysis is ongoing.
Start Year 2017
 
Description Semliki Forest 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Generated and analysed RNA-sequencing samples with bespoke in-house methods
Collaborator Contribution Generated samples
Impact The purpose of the collaboration is to study how arboviruses interact with their host cells with modern high-throughput techniques. No outputs yet
Start Year 2017
 
Description St Andrews 
Organisation University of St Andrews
Country United Kingdom 
Sector Academic/University 
PI Contribution Co-investigator on BBSRC sLoLa
Collaborator Contribution Lead-investigator on BBSRC sLoLa
Impact BBSRC sLoLa ongoing
Start Year 2012
 
Description Steve Goodbourn - St Georges - chicken innate molecule interactions. 
Organisation Ulster University
Department Biomedical Sciences Research Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution We have collaborated by producing yeast- 2-hybrid plasmids containing the influenza PB1-F2 gene from various avian influenza strains. The PhD student on this studentship then visited Professor Goodbourn's laboratory to carry out the yeast-2-hybrid screen for interaction innate chicken molecules.
Collaborator Contribution Professor Goodbourn's laboratory provided yeast-2-hybrid plasmids containing various innate chicken molecules to be screened for interaction with avian influenza virus PB1-F2 protein. They also offered help and guidance carrying out the interaction technique to understand if PB1-F2 directly associated with chicken innate components. They also provided reporter plasmids and guidance on innate reporter assays in chicken cells.
Impact Successful publication of work. doi: 10.1099/jgv.0.001220. The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells.
Start Year 2016
 
Description Suresh Kuchipudi and Janet Daly 
Organisation Penn State University
Country United States 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Suresh Kuchipudi and Janet Daly 
Organisation University of Nottingham
Department School of Veterinary Medicine and Science Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Thomas Bowden - STRUBI - University of Oxford - Glycoprotein structure collaboration 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data and advice about the biological properties of viral glycoproteins, e.g. their fusion capacity with various receptors, their role in determining host-range
Collaborator Contribution Advice and technical support at the structural level supporting future project development and design
Impact No outcomes as yet
Start Year 2018
 
Description Trevor Sweeney - University of Cambridge PhD Studentship 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of PhD studentship project in collaboration with Dr. Sweeney's lab at Cambridge and application at Pirbright.
Collaborator Contribution Development of PhD studentship project and application at Cambridge.
Impact The PhD studentship has been funded and is currently been advertised.
Start Year 2018
 
Description University of Liverpool Proteomics Collaboration 
Organisation University of Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Generation of samples to analyse the membranous proteome of cells infected with paramyxoviruses.
Collaborator Contribution Proteomic analysis of samples and technical support. Access to previous data-sets and expertise.
Impact Preliminary data sets only to date.
Start Year 2018
 
Description Vaccinia 
Organisation Defence Science & Technology Laboratory (DSTL)
Country United Kingdom 
Sector Public 
PI Contribution Generated and analysed DNA- and RNA-sequencing data from vaccinia samples with bespoke in-house pipelines
Collaborator Contribution Generated DNA and RNA vaccinia samples
Impact The collaboration aims to better understand the interaction between vaccina virus and its host(s), through the use of modern high-throughput techniques. No outputs yet
Start Year 2016
 
Description York 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration
Collaborator Contribution Collaboration
Impact Collaboration
Start Year 2015
 
Title Chicken IFITMs 
Description The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3). 
IP Reference PCT/GB2014/051693 
Protection Patent granted
Year Protection Granted 2018
Licensed Commercial In Confidence
Impact The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3). This patent has enabled us to leverage funding for continued work.
 
Description A talk at a symposium entitled: Using the 3Rs to support good science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry"
Year(s) Of Engagement Activity 2017
 
Description A talk at the British Poultry Diseases Group meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions.
Year(s) Of Engagement Activity 2017
 
Description AB Improving Approaches to Prevent and Control Viral Diseases of Livestock and Poultry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Batra, A., Maier, H.J., Britton P., Hiscox, J.A., Fife, M.S., 2015. PI3K/AKT signalling during infectious bronchitis virus infection. Food Security: Improving Approaches to Prevent and Control Viral Diseases of Livestock and Poultry, British Council Research Links Workshop, Istanbul, Turkey (Oral presentation)
Year(s) Of Engagement Activity 2015
 
Description AB Society of General Microbiology Annual conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Poster presentation. Batra, A., Maier, H.J., Britton, P., Hiscox, J.A., Fife, M.S. AKT activation during infectious bronchitis virus infection. Society of General Microbiology Annual conference, Liverpool, UK
Year(s) Of Engagement Activity 2013,2014,2015
 
Description Amy Lewis, The Scientist, 15/08/2017 re gene drive and Predator Free NZ 2050 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided information and quotes for article on potential use of gene drives as part of New Zealand effort to eradicate invasive predators
Year(s) Of Engagement Activity 2017
URL https://www.the-scientist.com/?articles.view/articleNo/50180/title/Driving-Down-Pests/
 
Description Ash Manor GCSE Mentoring 2018 - BA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Big Bang Fair South East - RC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Regional science festival/event - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Biotech YES, Syngenta Jealott's Hill, 05 Oct 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Teams of postgraduate students from a number of universities and institutes attended talk as part of Biotech YES (Young Entrepreneurs' Scheme). Follow-up discussion influenced several aspects of their approach to entrepreneurship both within the competition and more generally
Year(s) Of Engagement Activity 2014,2015,2016,2017
 
Description Broadcast on African swine fever on BBC wolrd service "The Food Chain" 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interview with BBC World Service "The Food Chain" on African swine fever virus.
Year(s) Of Engagement Activity 2018
URL https://www.bbc.co.uk/programmes/p05xhflj
 
Description CBD Open-ended online forum on synbio 06-10/2017 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact UN Convention on Biodiversity. Policy- discussion on synthetic biology: nominated participant in the Open Ended Online Forum on Synthetic biology as established in decision XII/24 of the Conference of the Parties to the Convention on Biological Diversity (COP) and extended in COP decision XIII/17 which ran from June to Oct 2017 (approx.)
Year(s) Of Engagement Activity 2017
URL http://bch.cbd.int/synbio/open-ended/discussion/
 
Description Camberley Library Fun Palace 2018 - JM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Caroline Seydel Genetic Literacy Project re GM mosquitoes/gene drive/Zika 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided background material and quotes for article on GM mosquitoes and control of Zika virus.
Year(s) Of Engagement Activity 2017
URL https://geneticliteracyproject.org/2018/01/29/waging-war-mosquito-split-gene-drive-hinder-spread-zik...
 
Description Centre of excellence for research on avian diseases (CERAD) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity 2017
 
Description Cheltenham Science Festival 2018 - JA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - JP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - RC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - SV 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Display at major science festival: changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018-DP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Conor Haydon participated in the STEM Day - Speed interviews and Careers Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact STEM day engagement and careers
Year(s) Of Engagement Activity 2018
 
Description Coronavirus lecture MSc students (Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A lecture was presented on the replication, cellular interactions and pathogenesis of coronaviruses to 15-20 MSc students from University of Surrey. The students were engaged and interested, answering and asking questions during the session.
Year(s) Of Engagement Activity 2016,2018
 
Description EUROPIC conference, The Netherlands, 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact EUROPIC conference, The Netherlands, 2018
Year(s) Of Engagement Activity 2018
 
Description Encouraging women into science and engineering STEM. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact MH Visited Warwick School, Redhill to talk to secondary students about careers in STEM. A very positive outcome and well received.
Year(s) Of Engagement Activity 2018
 
Description Ewen Callaway Nature 08/01/2018 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided expert background information for article in Nature regarding engineering species barriers, described by journalist as "very helpful" in followup email.
Year(s) Of Engagement Activity 2018
 
Description Expert group meeting for PPR eradication at head quarters of WHO, Vienna, Austria, 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact In the FAO/PPR eradication meeting satya has presented work on PPR epidemiology and diagnosis. The talk was designed for the stakeholders and policy make to make aware on important points that are required for eradication of PPR
Year(s) Of Engagement Activity 2018
 
Description Farnborough College Apprenticeship Fair- 20th March 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Careers fair attended by approx. 100 school children. Pirbright demonstrated the multiple ways that research organisations can provide jobs including scientists, engineers and computer operators.
Year(s) Of Engagement Activity 2018
 
Description Farnborough futures careers event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The careers event aimed to introduce the range of careers available at Pirbright. In addition I discussed my own career progression and highlighted the range of possible careers that I could have entered. Finally, I discussed some details of the research with interested students. The students had a wide range of backgrounds and career aims but were interested to hear about the Institute and the sort of work opportunities available. Several of them planned to follow up by investigating future job opportunities at Pirbright.
Year(s) Of Engagement Activity 2019
 
Description Flow cytometry workshop as part of the Bioimaging interest group 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Workshop at the Pirbright Institute - postdoctoral scientists of the project highlighted the use and application of flow cytometry in Virology
Year(s) Of Engagement Activity 2019
 
Description Helping Local Community Wildlife area 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Pond Clearing at Fox Corner Wildlife area (Hannah Goldswain)
Year(s) Of Engagement Activity 2018
 
Description Holt School Surrey. Bee meadow & A-level outreach Talk. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I discussed the importance of pollinators for biodiversity and food security with the younger students. I also discussed careers in science with A-Level students. There was good interaction and many questions surrounding both topics.
Year(s) Of Engagement Activity 2018
 
Description Influenza update 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Approx. 70 scientists attended a Influenza update meeting at which our group presented an oral presentation and poster presentation on our work. These actions resulted in establishment of new collaborations for ongoing work and the initiation of a joint grant writing exercise.
Year(s) Of Engagement Activity 2018
 
Description Initiation of a mass vaccination campain ( 2018) in TamilNadu, India 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Scientists from The Pirbright Institute's Vaccine Differentiation group have recently returned from Chennai in India, where they conducted a mass peste des petits ruminants (PPR) vaccination campaign and awareness programme. They joined scientists from four specialist organisations; Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Indian Veterinary Research Institute (IVRI), National Institute of Animal Biotechnology (NIAB) and National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI).
Peste des petits ruminants (PPR), also known as goat plague, is highly contagious and infects small ruminants such as sheep and goats, causing up to 90% mortality. The disease is prevalent across large parts of Africa, the Middle East, India and China and is estimated to cost between US$1.4 billion and US$2.1 billion globally each year. In 2017, the Food and Agricultural Organization (FAO) and the World Organisation for Animal Health (OIE) introduced a global eradication programme to reduce the devastating impact PPR has on the economy and food security of affected countries.
"Over 40 vets joined our vaccination campaign in the Tanir Kulum village of Tiruvallur District, TaminNadu (close to Chennai), where we administered vaccines to over 400 sheep and goats in a single day. We also ran an awareness camp where we provided farmers and vets alike with expert guidance on diagnosing clinical signs of PPR and what measures they could take to reduce its spread", said Professor Satya Parida from Pirbright, who led the collaborative effort with Dr Dhinakar Raj from TANUVAS.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-pes...
 
Description Innovate Guildford 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Represented the Pirbright Institute at Innovate Guildford Science Fair
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford 17 March 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Talk and exhibit at Innovate Guildford - >100 for talk, probably >500 for exhibit. Both sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford 2018 - JM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Regional science/innovation event - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford 2018 - SB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Regional science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford 2018 - SV 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Display at regional science/innovation festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford Outreach (12th March) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact With amazing exhibits, activities, workshops, talks and competitions, it's FREE to attend. Explore the four zones: Engineering, One Health, Digital Media and Future Living, experience interactive performances, hear inspiring speakers (including leading gaming pioneer Peter Molyneux and animal health pioneer Professor Nick Bacon) and take in an amazing atmosphere. Theatre, music and comedy will feature through the day, with everything from how technology might have affected Shakespeare's plays to risky recycling and photographic secrets.
Year(s) Of Engagement Activity 2017
URL https://www.guildford.gov.uk/innovateguildford
 
Description Innovate Guildford, ND 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Presented at the Pirbright Institute stand discussing science performed by the Institute with the general public. The stand focussed on an exhibit on avian influenza virus but more general virology topics were also discussed including our research.
Year(s) Of Engagement Activity 2017
 
Description Inreview with Daily Telegraph about African swine fever 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Interview with reporter from Daily Telegraph about African swine fever virus and risks to UK farmers
Year(s) Of Engagement Activity 2018
URL https://www.telegraph.co.uk/news/2018/12/12/uk-farmers-warned-alert-african-swine-fever-virus-hits-e...
 
Description International Avian Respiratory Disease Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Michael presented his research at the 2018 International Avian Respiratory Disease Conference in the US to an audience of avian researchers and representatives form the poultry veterinary vaccine industry. This sparked discussion and potential future collaborations
Year(s) Of Engagement Activity 2018
 
Description International Day of Women and Girls in Science 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I gave a quote as a STEM ambassador about why I think it is important to make sure women and girls have the same opportunities in STEM. This was shared on social media to celebrate International Day of Women and Girls in Science, a UN-led initiative.
Year(s) Of Engagement Activity 2019
 
Description International Women's Day video for social media 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Three team members were interviewed about their careers in science for International Women's Day. The videos were posted on Pirbright's website, Facebook and Twitter to celebrate what we love about working in science.
Year(s) Of Engagement Activity 2018
 
Description Interview with CNN on African swine fever virus 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interview with Nina Avramova from CNN International about African swine fever virus generally including transmission mechanisms and control strategies
Year(s) Of Engagement Activity 2018
URL https://edition.cnn.com/2018/10/02/health/african-swine-fever-europe-china-spread-intl/index.html
 
Description Interview with Health for Animals about African swine fever vrius vaccine development and current situation 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Interview with journalists from Health for Animals about African swine fever vaccine research published in a newsletter sent to 200 people.
Year(s) Of Engagement Activity 2018
URL https://healthforanimals.org/resources-and-events/newsletter-repository/17-disease-outbreaks.html?q=...
 
Description Invitation to give keynote talk on 'Control of avian diseases: Challenges and opportunities' 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave the Keynote talk on 'Control of avian diseases: Challenges and opportunities' at this International Symposium organized with an aim to gather international virology experts from both human and veterinary fields to share research experience, update findings and contribute their expertise through presentation and interactive discussion. The topics will cover a wide range of virology research common in both human and animal aspects. In addition, technological platforms such as vaccine development, and novel assays will be emphasized and addressed.
Year(s) Of Engagement Activity 2018
URL http://www.biotec.or.th/virus-symposium2018/index.php
 
Description Invitation to talk at the Vaccine Symposium "Vaccines at different levels of complexity from subunit to whole cell formulations" jointly organised by the Centre of Genomic Regulation (CRG), Radboud UMC and MSD Animal Health, with the support from the MycoSynVac project 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave a talk and participated in the discussion on the challenges and research opportunities for innovation in poultry disease control
Year(s) Of Engagement Activity 2017
URL http://www.crg.eu/
 
Description Invited Keynote Speaker International Society of Veterinary Epidemiology and Ecology, China Mai Thailand 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote talk on progress towards African swin efever virus vaccine development at ISVEE meeting Thailand
Year(s) Of Engagement Activity 2018
 
Description Invited seminar at UC Riverside 05/05/2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited seminar at University of California, Riverside
Year(s) Of Engagement Activity 2017
 
Description Invited speaker at the British VeterinaryPoultry Association 2018 meeting at Boehringer Ingelheim 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact I was invited to present my research findings at the British Veterinary Poultry Association 2018 meeting, held at Boehringer Ingelheim.
Year(s) Of Engagement Activity 2018
 
Description Invited talk at Agricultural Science congress at New Delhi, India, February-2019- Use of reverse genetics to study the early pathogenesis and to develop marker vaccines for PPR 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact 1. In a DBT-BBSRC FADH grant, The Pirbright Institute in collaboration with four Indian partners (TANUVAS, IVRI, NIAB and NIVEDI) has developed a PPR vaccine, which will be the first to differentiate between infected and vaccinated animals (DIVA), for which a patent application has been filed. Scientists have manipulated in the genome of PPR full length cDNA and rescued the DIVA vaccine virus using reverse genetic technique. Further this vaccine has been tested in goats providing full safety and potency upon virulent virus challenge. In contrast to current vaccine, this new DIVA vaccine can differentiate between naturally infected and vaccinated animals, therefore will help in meaningful assessment of vaccine coverage and epidemiological surveillance based on serology, in turn increasing the efficiency of control programmes.
2. Little is known about the early events in the development of peste des petits ruminants (PPR) virus (PPRV) infection. The current dogma is that similar to RPV, PPRV replicates primarily in the epithelium of the respiratory tract before disseminating throughout the host. However, our investigations, using intranasal inoculation of PPRV containing GFP (Green Fluorescent Protein) to mimic natural infection, indicate this is not the case and that immune cells in the pharyngeal tonsil is the primary target. This is a ground breaking discovery which changes the PPR pathogenesis. This infectious GFP virus is made from a synthetic cDNA of field virus (PPRV/Mor-08) using reverse genetics technique under FADH grant.
The presentation sparked questions and discussions after the talk. Two PPR DIVA vaccines developed and the mechanism of PPR virus pathogenesis explained.
Year(s) Of Engagement Activity 2019
 
Description Invited talk at the National Institute for Animal Biotechnology, Hyderabad on 3rd Jan 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Participants were engaged in the recent advances in biotechnology, challenges and opportunities.
Year(s) Of Engagement Activity 2019
 
Description Invited to talk at the International Conference on Building Human and Animal Health Capacities organised by the Jordan University of Science and Technology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Participated in the discussion and talked on 'The recent advances in vaccination-based control of poultry diseases'. The need for increased awareness of the pathogen diversity in the design of vaccines and the opportunities from advances in vaccine technologies were discussed
Year(s) Of Engagement Activity 2017
URL http://www.just.edu.jo/conferences/bhahc/Pages/default.aspx
 
Description JN - Mentoring 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Mentoring GCSE students to increase social mobility. Improving the confidence, ambition and interest in STEM subjects.
Year(s) Of Engagement Activity 2018
 
Description JN - Woking school 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Careers Fair at a local school encouraging children to consider science as a career and representing the institute in the local community.
Year(s) Of Engagement Activity 2008
 
Description Keynote talk at the World Veterinary Poultry Association meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited to give the keynote talk on 'Avian tumour viruses: their contribution to our understanding of cancer' at the World Veterinary Poultry Association XXth Congress in Edinburgh
Year(s) Of Engagement Activity 2017
URL http://www.wvpac2017.com/
 
Description Kingdown School Warminster Wiltshire talk to 6th form students about career in science. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I presented my career path to the students to engage them on STEM careers. I had a good level of discussion and many questions.
Year(s) Of Engagement Activity 2018
 
Description Lecture Series To Veterinary Medicine students School of Veterinary Medicine University of Surrey 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Undergraduate students
Results and Impact Lecture series in Virology to the BVetMed students at the School of Veterinary Medicine University of Surrey
Year(s) Of Engagement Activity 2017,2018
 
Description Lecture on entereic coronaviruses to MSc Students (Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A lecture was presented on the pathology, diagnosis and detection of enteric coronaviruses, including virus replication and interaction with the host. The students were engaged and asked/answered question throughout the session. We were invited to repeat the lecture for the subsequent student intakes.
Year(s) Of Engagement Activity 2015,2016,2018
 
Description Lecture to MSc studnets University of Surrey 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Lecture on African swine fever virus to MSc students University of Surrey Veterinary faculty
Year(s) Of Engagement Activity 2018
 
Description Lumpy Skin Disease D2R2 profile 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Provided expert opinion to the assessment (D2R2) of the risk of lumpy skin disease virus (LSDV) to the UK.
Year(s) Of Engagement Activity 2017,2018
 
Description MBPAV -presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Molecular Biology and Pathogenesis of avian viruses conference - Oxford 3-4th September 2018. Oral presentation given on drug resistant influenza viruses to approximately 50 scientist. Increased collaborative approaches following presentation.
Year(s) Of Engagement Activity 2018
URL https://microbiologysociety.org/event/society-events-and-meetings/focused-meeting-2018-molecular-bio...
 
Description MDV conference Tours 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact During the 11th International Symposium on Marek's Disease and Avian Herpesviruses, which took place from 6 to 9 July 2016, Paolo Ribeca gave a talk on "Attenuation of MDV: an RNA-seq based perspective".
Year(s) Of Engagement Activity 2016
 
Description MSc student visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I hosted several MSc students from University of Surrey, discussed avian research with them and demonstrated some laboratory techniques. The students gained an understanding of academic research and laboratory work.
Year(s) Of Engagement Activity 2018,2019
 
Description Microbiology Society Annual conference (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Poster or oral presentation at Microbiology Society annual conference, a major international event. The participants included researchers from many different areas of science. This provides a platform to promote research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014
 
Description Microbiology Society Avian Focus Meeting (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I gave a 15 minute oral presentation to an audience of around 80 people in 2016 and 2018. The audience consisted of researchers from many different scientific institutions from different areas of avian research. This was a great opportunity to present to experts in this field and gain novel insights into the project and the possible applications of my work.
Year(s) Of Engagement Activity 2016,2018
 
Description Microsoc 2018 "Structure-guided identification of a pathogen with pandemic potential" poster 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presented a poster on our research at the MicroSoc annual meeting
Year(s) Of Engagement Activity 2018
 
Description NL Next Generation Sequencing applications to improve livestock welfare, food security and socioeconomic stability in Brazil, British research Council, Newton Fund 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation: Next Generation Sequencing applications to improve livestock welfare, food security and socioeconomic stability in Brazil, British research Council, Newton Fund
Year(s) Of Engagement Activity 2015
 
Description Negative Strand Virus 2018 Meeting "Structure-guided identification of morbilliviruses with zoonotic potential" Poster 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presented a poster on the Structure-guided identification of morbilliviruses with zoonotic potential at the international negative strand virus (NSV) meeting in Verona, Italy.
Year(s) Of Engagement Activity 2018
 
Description Nick Rufford, Sunday Time Mag, published 27/08/2017 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Provided information and quotes for Sunday TImes Magazine article on GM mosquitoes and Zika
Year(s) Of Engagement Activity 2017
URL https://www.thetimes.co.uk/article/how-genetically-engineered-mosquitoes-will-save-lives-kwc8nmwh5
 
Description Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I presented my work at the Nidovirus Symposium, which is held every three years and brings together researchers from industry and academia from many different countries. I had some interesting discussions with other researchers and built my network.
Year(s) Of Engagement Activity 2017
 
Description Offer Paper oral presentation "Rapid Mammalian adaptation of H9N2 avian influenza viruses" at Microbiology society annual conference 4th April 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Offer Paper oral presentation "Rapid Mammalian adaptation of H9N2 avian influenza viruses" at Microbiology society annual conference 4th April 2017. Talk in the main virus symposia with an audience of approximately 200 researchers (PhD students through to Professors) mainly based in UK but some international. Discussions with other researchers around this area of work and increase in national profile of groups work.
Year(s) Of Engagement Activity 2017
 
Description Oral and poster presentation at European Society of veterinary Virology and EPIZONE (ESVV/EPIZONE) at Vienna, Austria 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation has been made on early events of PPR virus pathogenesis. The presentation reveled that PPR virus first taken up by the immune cells to the Pharyngeal tonsil and then to other lymph nodes before generalisation of the disease. Secondary replication happens in epithelial cells. This alters the existing believe that virus primarily replicate in the epithelial cells.
Similarly a poster presentation has been done to show the detection of nucleic acid of PPR virus during PPR eradication programme, nasal sample has been shown as the best sample for viral genome detection.
Year(s) Of Engagement Activity 2018
 
Description Oral presentation at the Global Alliance for Research on Avian Disease meeting in Hanoi, Vietnam 15th -19th Jan 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact A talk was given on the PB1-F2 function in avian influenza infection of avian hosts at the GARAD meeting. This meeting was attended by approximately 120 researchers and poultry industry members from a wide international geographic region.
Year(s) Of Engagement Activity 2018
 
Description Oral presentation to Large Animal Research Network 24th May 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Talk about work understanding PB1-F2 function in avian hosts highlighting how the animal work carried out was undertaken and monitored. this was delivered to an audience of researchers, funders and industry interested in animal model systems for research and the 3Rs commitment surrounding this work. Discussions with interested parties about our chicken work at the Institute and the diverse way that the birds are entertained whilst being housed (e.g. live food, vegetables, perches, dust baths etc).
Year(s) Of Engagement Activity 2017
 
Description Oral presentation, Global Foot-and-Mouth (F M D) Research Alliance (G F R A) conference, Korea, 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Oral presentation, Global Foot-and-Mouth (F M D) Research Alliance (G F R A) conference, Korea, 2017
Title: The Development of New Master Vaccine Seed Stocks for FMD Control in East Africa
Year(s) Of Engagement Activity 2017
 
Description Oral preserntation at Influenza update Meeting in Edinburgh 21st November 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Presentation of "Rapid Mammalian adaptation of H9N2 virus" to Influenza researchers attending the 2 day Influenza viruses meeting at the Roslin institute in Edinburgh.
Year(s) Of Engagement Activity 2017
 
Description Organisation of Influenza meeting at Roslin Institute 21st-22nd November 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Along with Paul Digard (Edinburgh) and Edward Hutchinson (Glasgow) I helped to organize, plan and run a Influenza update meeting which ran for 2 days at The Roslin Institute in November 2017. The symposium was attended by approximately 60 influenza researchers from the UK. Talks were given by a range of people with student talks and posters being awarded prizes. This was supported by Medimmune and the Microbiology Society.
Year(s) Of Engagement Activity 2017
 
Description Organisation of UK-India Bioinformatics Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The meeting discussed the challenges and opportunities and have decided to come up with a strategy document on working together between UK and India on data-driven biology
Year(s) Of Engagement Activity 2018
 
Description Organised the 3rd UK-China CERAD meeting in Guildford, UK in July 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The 3rd UK-China CERAD meeting helped to bring together professional from both countries to identify challenges and exploit the opportunities for improved control of poultry diseases and increasing food security.
Year(s) Of Engagement Activity 2017
URL http://www.uk-china-cerad.org/
 
Description Organiser of the Animal Genetics and Diseases conference 08 - 10 May 2019 Wellcome Genome Campus, UK. Highlighting recent advances in animal genetics and genomic technologies. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I am on the organising committee for this conference. The second conference in this series will highlight recent advances in animal genetics and genomic technologies. It will bring together specialists working on the interface of genomics, genetic engineering and infectious disease with the aims of improving animal and human health and welfare.

Novel genomic technologies, mathematical modelling and quantitative genetics approaches, applied to host animals, as well as their pathogens, have transformed the understanding of animal diseases, host-pathogen interactions and epidemiology and their effects on productivity of farmed animal species and food supply chains.

This year's conference will not only put the spotlight on the immune response of host animals and epidemiology but also cover the genetics and genomics of pathogens and the impact of animal-human relationships.

We encourage registrations from researchers, breeders and technical specialists interested in learning and disseminating the latest cutting-edge techniques and methodologies across model species, wildlife, farmed animals and companion animals.
Year(s) Of Engagement Activity 2019
URL https://coursesandconferences.wellcomegenomecampus.org/our-events/animal-genetics-diseases-2019/?dm_...
 
Description Organising and giving Keynote talk at the GARAD meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Global Alliance of Poultry industry professional and academia to discuss about the advances in control strategies of diseases
Year(s) Of Engagement Activity 2018
URL http://Garad.org
 
Description PPR eradication meeting organised by FAO and OIE at Rome and talk has been delived on Development of DIVA vaccines 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Satya Parida is working as an expert to PPR eradication and he is being invited for few of FAO/OIE meetings. The meetings discussed about roadmap, vaccine production capacity and eradication policy.
Satya also presented work on PPR DIVA vaccine and DIVA tests developments and their evaluation in goats.
Year(s) Of Engagement Activity 2018
 
Description PPR-Global Research and Expertise Network talk "Examining the zoonotic potential of PPRV" Vienna 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Gave a talk to the inaugural meeting of the PPR Global Research and Expertise Network (PPR GREN) at IAEA headquarters in Vienna, Austria. Participants included policy makers from the FAO and OIE.
Year(s) Of Engagement Activity 2018
 
Description Pig and Poultry Fair 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Discussed Institute research with pig and poultry farmers, related industry and the general public. Received requests for more information related to research, business and studentships.
Year(s) Of Engagement Activity 2018
URL https://www.pigandpoultry.org.uk/
 
Description Poster presentation (two posters) EuFMD conference, Italy, 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster presentation (two posters) EuFMD conference, Italy, 2018
1). Development of Master Vaccine Seeds for Foot-and-Mouth Disease Control in Sub-Saharan Africa
2). Chimeric SAT2 with improved thermostability as an FMD vaccine candidate
Year(s) Of Engagement Activity 2018
 
Description Poultry Health course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poultry Health Course is run as a residential and online course to transfer recent advances in avian disease research from the laboratory to the field.
Year(s) Of Engagement Activity Pre-2006
URL http://www.poultryhealthcourse.com/
 
Description Presentation at Avian viruses focussed meeting - Sept 2018 ND 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation of data as an offered paper at the molecular biology and pathogenesis of avian viruses meeting, Oxford Sept 2018.
Year(s) Of Engagement Activity 2018
 
Description Presentation at Global Alliance for Research on Avian Diseases Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation of my research at the Global Alliance for Research on Avian Diseases Conference in Vietnam to approximately 80 conference participants resulted in discussions about future research directions.
Year(s) Of Engagement Activity 2018
 
Description Presentation at Inter-institute Bioimaging networking meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact An event was held to enable improved collaboration opportunities between scientists working with bioimaging techniques at the different research institutes within the UK. I presented data from the last 10 years describing our work characterising replication organelles induced by IBV, describing the different techniques and approaches we have used and the outlook for the future. The talk aimed to showcase the bioimaging facilities at Pirbright to encourage new collaborations.
Year(s) Of Engagement Activity 2018
 
Description Presentation at RIVR meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Oral presentation of my work to other researchers at the Recently Independent Virology Researchers meeting. I discussed my data with other researchers and potential for future collaboration.
Year(s) Of Engagement Activity 2016,2017,2019
 
Description Presentation at RIVR meetings 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Presentation of my research at Recently Independent Virology Researchers (RIVR) meetings in 2016, 2017, 2018 and 2019. I discussed my work and future collaborations with other virology researchers working in the UK.
Year(s) Of Engagement Activity 2016,2017,2018,2019
 
Description Presentation by ND at Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact The post-doctoral researcher presented our data at the 14th international Nidovirus symposium. She discussed data and potential collaboration with other scientisits from the field.
Year(s) Of Engagement Activity 2017
 
Description Presentation by RH at Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact PhD student presented our data at the 14th international Nidovirus symposium. He discussed results and potential for future collaboration with other scientists in the field.
Year(s) Of Engagement Activity 2017
 
Description Presentation of project results at the 13th International dsRNA Virus Symposium 24th-28th of September 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster and Oral presentations included key results of the studentship project
Year(s) Of Engagement Activity 2018
 
Description Presentation to BBSRC Executive Board 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact I presented our research to the BBSRC Executive Board when they visited The Pirbright Institute in September 2018 and discussed the impacts of our work..
Year(s) Of Engagement Activity 2018
 
Description Project close meeting at TANUVAS, Chennai July 2018- Delivering talk on outcome of the project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact The project team held their final workshop at TANUVAS, which concluded a four year Farmed Animal Disease and Health (FADH) grant joint funded by the Biotechnology and Biological Sciences Research Council (BBSRC) and the Indian Government's Department of Biotechnology (DBT). "This grant has enabled us to collaborate with four organisations across India, and ensured that our research can be applied in the field to aid the campaign for PPR global eradication", said Professor Parida.The project covered many areas of research which are essential for understanding PPR and creating tools to help control and prevent the disease. The team have now filed a patent application for their newly developed PPR vaccine, which is the first to differentiate between vaccinated and infected animals (DIVA) - a quality that enables livestock owners to protect their animals whilst continuing to trade.
The team have also investigated how the PPR virus (PPRV) infects sheep and goats and how their immune systems respond. By inserting green fluorescent protein into virulent PPRV and administering the modified virus to goats, they demonstrated that PPRV primarily infects the tonsils, challenging the earlier belief that the virus first replicates in the respiratory tract epithelial cells. The collaborative project has also generated better diagnostic tests for use in the field and laboratory, and preliminary research has identified why some Indian breeds of goats and sheep are resistant to the disease, which could help scientists to create PPRV resistant breeds in the future.
Project partners, scientists from the University and 40 field veterinarians have joined the meeting. An awareness training has been conducted on PPR disease and eradication for these field veterinarians.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-pes...
 
Description Provided information to Podcast This week in Virology about African swine fever virus spread in wild boar 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact E-mail correspondence providing advice to presenters of TWIV This week in Virology Podcast.
Year(s) Of Engagement Activity 2019
URL http://www.microbe.tv/twiv/twiv-532/
 
Description Reverse genetics seminar (University of Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A seminar was given based upon the reverse genetics system of infectious bronchitis virus. The audience was a group of 10 -15 MSc students from University of Surrey. The students were engaged, asked questions about the research and careers in science. We were asked to repeat the seminar for the next year's intake of students.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017
 
Description Reverse genetics seminar to MSc students 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact A seminar was presented describing reverse genetics of viruses, including infectious bronchitis virus and the application of reverse genetics in research. The students were interested and engaged, participating in activities and asking questions. We were invited to present the seminar in subsequent years for new student intakes.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2018
 
Description Royal Institution Christmas Lectures Family Day - LA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Six Pirbright staff/students ran an all-day exhibit on gene editing and mosquitoes at the Royal Institution About 270 participants visited our stand; wide range of comments, questions, discussion and feedback
Year(s) Of Engagement Activity 2019
 
Description Royal Institution Christmas Lectures Family Day - SB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact About 270 participants visited our stand; wide range of comments, questions, discussion and feedback
Year(s) Of Engagement Activity 2019
 
Description Royal Society of Biology Biology week talk, Winchester, 10/10/2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Talk organized by Royal Society of Biology as part of Biology Week. This one in Winchester, audience was general public with strong representation of school-age. Questions and discussion during and after formal talk.
Year(s) Of Engagement Activity 2017
 
Description STEM Day - Speed interviews and Careers Day 2018 - BA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description STEM Outreach Event (Farnborough, 9th November) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact See web link
Year(s) Of Engagement Activity 2016
 
Description Seminar given at The Roslin Institute - 19th October 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact Invited to give a Seminar on PB1-F2 programme of work at The Roslin Institute in Edinburgh to researchers.
Year(s) Of Engagement Activity 2017
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presentation on African swine fever virus vaccine development to Yebio Bioengineering and China Animal Health and Epidemiology Centre, Qingdao, PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar on African swine fever virus host interactions and vaccine development. Yangzhou University Veterinary Faculty PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar at Beijing Institute of Animal Sciences, Chinese Academy of Agricultural Sciences 12 Zhongguanchan South Stree, Haifing District Beijing
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus virus host interaction and vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus virus host interaction and vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Seminar on African swine fever virus evasion of host defences and vaccine development Institute of Molecular and Cellular Biology, UCL London
Year(s) Of Engagement Activity 2018
 
Description Seminar on prospects for African swine fever virus vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar at Harbin Institute for Veterinary Research, PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on virus host interactions and prospects for African swine fever virus vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Seminar on African swine fever virus host interactions and vaccine development
Year(s) Of Engagement Activity 2018
 
Description Stacey Human - University of Surrey Open Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The postdoctoral researcher in my team took part in the University of Surrey Open Day advertising the work of The Pirbright Institute
Year(s) Of Engagement Activity 2018
 
Description Sue Baigent participated in the Teen Tech event on 22nd Jan 2019 on General Science engagement 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact A General Science event
Year(s) Of Engagement Activity 2019
 
Description Sue Baigent participated in the Winston Churchill Careers Fair on 7th Nov 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Careers fair
Year(s) Of Engagement Activity 2018
 
Description Surrey County Show 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact The Surrey County Show is an agricultural show in Guildford that is visited by the general public. The Pirbright Institute had a stand with various activities for visitors to take part in and explain our science to a wide audience. Giulia spent a day working on the stand and spoke with many children and adults about her work at Pirbright to change public attitudes and stimulate increased interest in research.
Year(s) Of Engagement Activity 2018
 
Description Surrey vet school open day - MJB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact The event was an open day for prospective students for the University of Surrey vet school. The course includes a research placement, which can be conducted at Pirbright. Therefore there was a stand from Pirbright to describe the research we perform generally as well as a discussion of our specific research. The audience were generally interested and the information helped inform them when selecting a university course.
Year(s) Of Engagement Activity 2018
 
Description TK School activity - Teentech Surrey (15 Nov 17) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Event where upwards of 100 school children aged 11-14 years attend from the regional area and complete STEM based challenges. Influenza virus based challenge set. This event has a measured impact in encouraging children to participate in STEM opportunities particularly girls and those in disadvantaged backgrounds.
Year(s) Of Engagement Activity 2017
URL http://www.teentech.com/
 
Description TW ARC 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact ARC will hold up to two dissemination events per annum. The aim of these events is to facilitate the dissemination of the research findings from the grants awarded through ARC to the Industry Members of the Club and other researchers. The dissemination events also provide an excellent opportunity for networking and we encourage delegates to make the most of the time made available for this purpose in the programme. BBSRC staff and the ARC coordinators will be available throughout the day should you have any questions regarding the Club.
Presentations: The presentations are the main opportunity for ARC research groups to present research findings and future plans and for Industrial Members to find out about ARC funded projects and provide feedback. Featured projects have been allocated presentation time as described in the programme and should include at least 5 minutes for questions. Presenters are asked to keep to time and presentations should demonstrate the relevance of the work to animal health and the livestock industry.
Posters: Research groups funded through ARC have been offered the opportunity to provide posters of work which is of relevance to the Industry Members of the Club. This will provide an opportunity to have informal discussions about current progress during networking activities and provide additional project information of relevance to ARC.
Year(s) Of Engagement Activity 2015,2016
 
Description TW Oxford Interdisciplinary Bioscience Networking Event 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Oxford Interdisciplinary Bioscience Networking Event
Year(s) Of Engagement Activity 2015
 
Description Taiwan -UK Partnering Award: Surveillance of influenza viruses 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Attended the International Flu Virus Symposium for Celebrating IPM 47th Anniversary" in Tri-Service General Hospital in Taipei, Taiwan.

Tri-Service General Hospital address:
No. 325, Sec. 2, Chenggong Rd., Neihu Dist., Taipei City 114, Taiwan (R.O.C.)

Invited speaker for medical staff and research scientists.
Year(s) Of Engagement Activity 2019
URL https://www.cdc.gov.tw/rwd/english
 
Description Talk as part of the University of Veterinary Medicine (Vienna) doctoral school "Infectious Diseases of Pig and Poultry" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Talked about the Pirbright Institute's work on African swine fever virus to researches in Veterinary Medicine (Virology and Immunology) in Vienna.
Year(s) Of Engagement Activity 2018
 
Description Talk at Avian viruses focussed meeting - Sept 2018 HM 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation of data as an offered paper at the Molecular biology and pathogenesis of avian viruses meeting in Oxford, September 2018.
Year(s) Of Engagement Activity 2018
 
Description Teaching- lecturing, small group classroom teaching and laboratory practical teaching 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact I have been actively engaged with teaching activities at the University of Surrey, giving lectures, small group classes and laboratory practical classes to vet students and MSc students.
Year(s) Of Engagement Activity 2015,2016,2017
 
Description Teentech 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The annual Teentech student science event in Guildford aims to encourage teenagers to consider science subjects and science careers. Our stand had considerable interest from the schoolchildren who were very enthusiastic. The event stimulated an increased interest in science and research.
Year(s) Of Engagement Activity 2016,2019
 
Description Teentech Guildford - ND 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Teentech is an event held for students from a range of local school who have an interest in science to experience the range of scientific activities that could be open to them for future careers. Students in small groups rotate around stands at the event to perform activities at each station, guided by scientists. The Pirbright stand was demonstrating the principle of genetic modification of mosquitos. The children were very interested in the exhibit and asked lots of questions.
Year(s) Of Engagement Activity 2013,2019
 
Description Television interview Ulaanbaatar 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact I was interviewed by UB television while on a collaborative visit to the State Central Veterinary Laboratory in Mongolia. I spoke about the collaboration between The Pirbright Institute and the SCVL, and how this benefits the Mongolian agricultural sector.
Year(s) Of Engagement Activity 2017
 
Description The Hurst careers event - HJM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The Hurst school held a careers event for GCSE students to attend throughout the day. The Pirbright stand contained a variety of engagement activities to demonstrate the research we perform and to stimulate discussion. The students seemed interested in the activities and those more interested in a science career asked a variety of questions about the research and entry into scientific research as a career.
Year(s) Of Engagement Activity 2018
 
Description This Week In Virology Podcast 532: Morbillivirus had a little lamb 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Our research paper on morbillivirus host-range in Journal of Virology was highlighted for in-depth discussion on this episode of TWIV where they spent 30+ minutes talking about the work and its potential impact. This podcast is the number one podcast in virology and listened to across the globe.
Year(s) Of Engagement Activity 2019
URL http://www.microbe.tv/twiv/twiv-532/
 
Description This week in Virology: 533: Recurring threads 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Following our paper being highlighted on the previous edition of TWIV I wrote a letter to further the discussion of this subject and clarify some technical points on The Pirbright Institute. This was read out as part of the podcast and discussed.
Year(s) Of Engagement Activity 2019
URL http://www.microbe.tv/twiv/twiv-533/
 
Description Tillingbourne School bug hunt - ND 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Primary school students were involved in an event to identify insects in the school grounds.
Year(s) Of Engagement Activity 2018
 
Description Tillingbourne School science event - ND 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Tillingbourne School held a science event for year 3 students to explore different aspects of science including smell, acid & alkali, magnetism etc. Nicole was based at the microscopy station helping children to view samples under the microscope, explain what they were looking at and answer any questions to children asked. The children were highly engaged and interested in the exhibit.
Year(s) Of Engagement Activity 2018
 
Description Tillingbourne Year 3 Science Day 2018 - EL 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Tillingbourne bug hunt. New Rd, Chilworth, Guildford GU4 8NB 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research. Good interest and engagement.
Year(s) Of Engagement Activity 2018
 
Description Tour of Pirbright Research facilities for CLA group 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The CLA is the membership organisation for owners of land, property and businesses in rural England and Wales. The CLA came for a tour of the Jenner building facilities at The Pirbright Institute. I led a tour of our building for the group and described the work undertaken by the avian research programme here.
Year(s) Of Engagement Activity 2017
 
Description U3A talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Presentation on influenza virus research at The Pirbright Institute for the local University of the Third Age group, approx. 50 people aged 65 years + all with an interest in science.
Year(s) Of Engagement Activity 2018
URL https://cu3asandt.wordpress.com/2018/09/23/meeting-2nd-october/
 
Description UK-India bioinformatics workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact On February 9 2018 Pirbright organised a "UK-India bioinformatics workshop" in order to foster ties between Indian and UK bioinformaticians working in the field of livestock and crop research. Paolo Ribeca gave a talk on "Bioinformatics and sequencing at Pirbright".
Year(s) Of Engagement Activity 2018
 
Description V Nair invited to talk on 'Cell type- and disease stage-specific interactions of Marek's disease virus & the host' at the International Symposium on Marek's disease and avain herpesiviruses at Yangzhou, China during 29th July to 2nd Aug 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Most recent updates in the field of avian herpesvirus research was discussed and active engagements for future collaborations discussed
Year(s) Of Engagement Activity 2018
 
Description Veterinary Times article on morbillivirus vaccines. 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was interviewed by a journalist from The Veterinary Times about our recent Journal of Virology research paper on morbillivirus host-range. This was then written up for the next edition of this publication.
Year(s) Of Engagement Activity 2019
URL https://www.vettimes.co.uk/news/scientists-multi-purpose-ppr-vaccine-closer-to-completion/
 
Description Winston Churchill Careers Fair. Winston Churchill School, Hermitage Road, St Johns, Woking, Surrey, GU21 8TL 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact AS attended and presented at this event to stimulate increased interest in science and research.
Year(s) Of Engagement Activity 2018
 
Description World Zoonosis Day 2018 - web story 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Contributed to an article from our institute on World Zoonosis Day highlighting the importance of research in this area.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/07/pirbright-scientists-are-developing-vaccine-candidates-anim...
 
Description a talk at the Recently Independent Virology Investigators meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talks outlining my current research and future plans.
Year(s) Of Engagement Activity 2015,2016,2017
 
Description email interview with journalist (Sukanya Charuchandra, The Wire) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Email interview for journalist for "The Wire" - an online technology-focused media outlet. Helped provide context and impact for the resulting piece. https://thewire.in/the-sciences/scientists-identify-protein-that-mosquitoes-need-to-lay-viable-eggs
Year(s) Of Engagement Activity 2019
URL https://thewire.in/the-sciences/scientists-identify-protein-that-mosquitoes-need-to-lay-viable-eggs
 
Description organised workshops at Microbiology Society annual conferences 2014 - 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I co-organised virology division workshops on viral evolution and diversity and RNA viruses with colleagues from the virology division. The workshops were held at the annual Microbiology Society conferences in spring each year. Organisation involved selecting abstracts for oral or poster presentation, ordering the presentations and chairing the sessions.
Year(s) Of Engagement Activity 2015,2016,2017,2018