Direct writing of micrometer arrays of biomolecules on diamond and diamond-like-carbon surfaces

Lead Research Organisation: University of Bristol
Department Name: Chemistry

Abstract

This project couples two different state-of-the-art concepts in the fabrication of protein or DNA modified surfaces: the manufacturing of microstructured arrays of biomolecules via the laser induced forward transfer (LIFT) technique, and the usage of diamond and diamond-likecarbon (DLC) surfaces as platform for deposition. The laser induced forward transfer technique is a direct write, non-contact printing technique that was originally used for printing of metals and electronic components. Recently, this technique has been also used for printing of patterned arrays of DNA and biomolecules. Further analysis of the deposited micropatterns shows no degradation of the biomolecules within the solution, making this a viable alternative for more classical technique for micrometer array deposition of biomolecules. This deposition technique will be coupled with the usage of functionalised diamond coatings as the platform for biomolecule immobilisation. DNA and protein functionalisation of diamond has been recently illustrated to be possible. Indeed, DNA arrays deposited on diamond showed excellent stability. The combination of the two will produce stable microarrays of biomolecules on a diamond or diamon-like-carbon surface.

Publications

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Related Projects

Project Reference Relationship Related To Start End Award Value
EP/C532066/1 01/01/2006 31/07/2008 £157,001
EP/C532066/2 Transfer EP/C532066/1 01/08/2008 31/12/2008 £24,313
 
Description This project explored Laser Induced Forward Transfer as a direct write technique to deposit 2D patterns. We achieved depostion of metal-oxide based patterns (ZnO) and we reported on the patterned growth of ZnO nanotubes. We have also successfully explored the depostion of biomolecules with this technique (proteins and polysaccharides). Addtionally we explored Diamond-Like Carbon as a biocompatible surface and specifically studied neuronal cell growth on these surfaces. We have been able to tune and guide the growth of primary neurons and the outgrowth of Dorsal Root Ganglions, which was reported in two high-impact publications.
Exploitation Route - Coatings for implants

- Printing techniques for conducting polymers

- Printing techniques for tissue engineering applications Currently, we are investigating the use of the Laser Induced Forward Transfer as an alternative printing technique to Inkjet printing for printing conducting polymers, biomaterials and ceramics.

The DLC thin film coatings are also further investigated for coatings of neural implants and brain-computer interfaces.
Sectors Electronics,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description The findings have been used as a precursor for a number of research proposals, one of which successful. Also the findings enabled a umber of international collaborations.
First Year Of Impact 2009
Sector Healthcare,Manufacturing, including Industrial Biotechology
Impact Types Economic

 
Description EPSRC responsive mode
Amount £298,000 (GBP)
Funding ID EP/K002503/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 01/2013 
End 12/2016
 
Description NIHR
Amount £125,000 (GBP)
Funding ID ll-FS-0909-13096 
Organisation National Institute for Health Research 
Sector Public
Country United Kingdom
Start 10/2010 
End 10/2011
 
Description Scaffolds for Neural tissue engineering
Amount £98,277 (GBP)
Funding ID EP/I007695/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 10/2010 
End 10/2012