Direct writing of micrometer arrays of biomolecules on diamond and diamond-like-carbon surfaces
Lead Research Organisation:
University of Bristol
Department Name: Chemistry
Abstract
This project couples two different state-of-the-art concepts in the fabrication of protein or DNA modified surfaces: the manufacturing of microstructured arrays of biomolecules via the laser induced forward transfer (LIFT) technique, and the usage of diamond and diamond-likecarbon (DLC) surfaces as platform for deposition. The laser induced forward transfer technique is a direct write, non-contact printing technique that was originally used for printing of metals and electronic components. Recently, this technique has been also used for printing of patterned arrays of DNA and biomolecules. Further analysis of the deposited micropatterns shows no degradation of the biomolecules within the solution, making this a viable alternative for more classical technique for micrometer array deposition of biomolecules. This deposition technique will be coupled with the usage of functionalised diamond coatings as the platform for biomolecule immobilisation. DNA and protein functionalisation of diamond has been recently illustrated to be possible. Indeed, DNA arrays deposited on diamond showed excellent stability. The combination of the two will produce stable microarrays of biomolecules on a diamond or diamon-like-carbon surface.
Organisations
Publications
Klini A
(2007)
ZnO nanorod micropatterning via laser-induced forward transfer
in Applied Physics A
Claeyssens F
(2007)
Laser patterning of Zn for ZnO nanostructure growth: Comparison between laser induced forward transfer in air and in vacuum
in Thin Solid Films
Howie WH
(2008)
Characterization of solid-state dye-sensitized solar cells utilizing high absorption coefficient metal-free organic dyes.
in Journal of the American Chemical Society
Kelly S
(2008)
Patterned growth of neuronal cells on modified diamond-like carbon substrates.
in Biomaterials
Prescott, Wesley V.; Schwartz, Arnold I.
(2008)
Nanorods, Nanotubes and Nanomaterials Research Progress
Frederik Claeyssens (Author)
(2009)
CELL PATTERNING ON MODIFIED DIAMOND-LIKE CARBON (DLC)
Claeyssens F
(2009)
Three-dimensional biodegradable structures fabricated by two-photon polymerization.
in Langmuir : the ACS journal of surfaces and colloids
Claeyssens F
(2010)
Design of three-dimensional solid-state boron oxide networks: Ab initio calculations using density functional theory
in Physical Review B
Frederik Claeyssens (Author)
(2010)
Three-dimensional Polycaprolactone Structures Fabricated by Two-Photon Polymerization
Description | This project explored Laser Induced Forward Transfer as a direct write technique to deposit 2D patterns. We achieved depostion of metal-oxide based patterns (ZnO) and we reported on the patterned growth of ZnO nanotubes. We have also successfully explored the depostion of biomolecules with this technique (proteins and polysaccharides). Addtionally we explored Diamond-Like Carbon as a biocompatible surface and specifically studied neuronal cell growth on these surfaces. We have been able to tune and guide the growth of primary neurons and the outgrowth of Dorsal Root Ganglions, which was reported in two high-impact publications. |
Exploitation Route | - Coatings for implants - Printing techniques for conducting polymers - Printing techniques for tissue engineering applications Currently, we are investigating the use of the Laser Induced Forward Transfer as an alternative printing technique to Inkjet printing for printing conducting polymers, biomaterials and ceramics. The DLC thin film coatings are also further investigated for coatings of neural implants and brain-computer interfaces. |
Sectors | Electronics Healthcare Pharmaceuticals and Medical Biotechnology |
Description | The findings have been used as a precursor for a number of research proposals, one of which successful. Also the findings enabled a umber of international collaborations. |
First Year Of Impact | 2009 |
Sector | Healthcare,Manufacturing, including Industrial Biotechology |
Impact Types | Economic |
Description | EPSRC responsive mode |
Amount | £298,000 (GBP) |
Funding ID | EP/K002503/1 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2013 |
End | 12/2016 |
Description | NIHR |
Amount | £125,000 (GBP) |
Funding ID | ll-FS-0909-13096 |
Organisation | National Institute for Health Research |
Sector | Public |
Country | United Kingdom |
Start | 09/2010 |
End | 10/2011 |
Description | Scaffolds for Neural tissue engineering |
Amount | £98,277 (GBP) |
Funding ID | EP/I007695/1 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2010 |
End | 10/2012 |