Developing the NanoKick bioreactor to enable tissue engineered bone graft and use of metabolomics to identify bone specific drug candidates

Lead Research Organisation: University of the West of Scotland
Department Name: School of Engineering

Abstract

Bone graft is regularly used in surgery (plastics, maxillofacial surgery and orthopaedics); bone is actually the second most grafted tissue after blood. Ideally the surgeon wishes to take bone from one area (donor site) to another area (recipient site) to support the operation they are performing. However, a patient's own donor bone is in short supply and its removal can lead to complications in the donor site. This means the surgeon will often recourse to allograft - decellularised (and thus biologically inferior) - bone from other people. A third, and growing, option is synthetic graft. Synthetic graft can be made from biologically active materials, but is not viable and thus not yet as good as living bone.
Our bioreactor, that supplies nanoscale 'kicks' to cells in culture can be used to convert mesenchymal stem cells (the stem cells of the bone, simple to isolate from a patient's iliac crest or fat tissue) to bone forming osteoblasts. It can achieve this with cells seeded into 3D environments such as gels or potentially synthetic graft materials. This thus allows us to envisage supply of living bone graft derived from a patient's own cells. The ability to supply such materials would provide a new gold standard for bone grafting.
In this project we will thus develop our bioreactor into a flexible platform for study of bone regeneration (which will also be of significant interest to many academic labs in the field) and provision of bone graft.

Further to this vision of tissue engineered bone supply, there is also a big need in Pharma for relevant bone models to reduce use of both standard lab models that are very dissimilar to the in-body environment and animal testing which has large cost and ethical consideration. Our ability to produce 3D bone in the lab simply, reproducibly, at low cost and without need for chemical control of cell phenotype (we will just use the nanokicks) will provide an excellent model for testing of drugs for e.g. osteoporosis, osteogenesis imperfecta and other bone conditions. In this project, we will use our technique to study 3D bone formation in the lab and look at what metabolites, the basic building blocks of life, the cell use as they form bone. We will then identify bioactive metabolites and validate them in our bone mimics.

Finally, we will test to see feasibility of applying nanokicks to humans to help treat e.g. spinal injury, slow bone repair and osteoporosis etc. We will move from mechanical nanokicks to acoustic nanokicks to achieve this.

Publications

10 25 50

Related Projects

Project Reference Relationship Related To Start End Award Value
EP/N012631/1 01/02/2016 31/08/2017 £290,447
EP/N012631/2 Transfer EP/N012631/1 01/09/2017 31/01/2019 £79,618
 
Description Progression of Nanokick technology to the point where patent submitted (GB201514734A) and the first in man study has been funded, see:
http://www.findabetterway.org.uk/news/synthetic-bone-project-full-swing/
Exploitation Route Progression of Nanokick technology to the point where patent submitted (GB201514734A) and the first in man study has been funded, see:
http://www.findabetterway.org.uk/news/synthetic-bone-project-full-swing/
Sectors Healthcare,Pharmaceuticals and Medical Biotechnology

URL http://www.nanokick.com
 
Description Progression of Nanokick technology to the point where patent submitted (GB201514734A) and the first in man study has been funded, see: http://www.findabetterway.org.uk/news/synthetic-bone-project-full-swing/ In addition, significant engagement with clinicians and in public engagement, e.g.: https://royalsociety.org/science-events-and-lectures/summer-science-exhibition/exhibits/stem-cells-nano-kicking/
First Year Of Impact 2016
Sector Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Societal

 
Description GLORI - Glasgow Orthopaedic Research Initiative 
Organisation Queen Elizabeth University Hospital
Country United Kingdom 
Sector Hospitals 
PI Contribution Presented invited talk at 2018 conference.
Collaborator Contribution Linking with clinicians working on orthopaedics to support translation of biomedical research (particularly Nanokicking) to clinic.
Impact Strengthening of collaborations which are associated with nanokicking grants.
Start Year 2009
 
Description GLORI - Glasgow Orthopaedic Research Initiative 
Organisation University of Glasgow
Country United Kingdom 
Sector Academic/University 
PI Contribution Presented invited talk at 2018 conference.
Collaborator Contribution Linking with clinicians working on orthopaedics to support translation of biomedical research (particularly Nanokicking) to clinic.
Impact Strengthening of collaborations which are associated with nanokicking grants.
Start Year 2009
 
Description Scottish Centre for Innovation in Spinal Cord Injury (SCISCI) 
Organisation Queen Elizabeth University Hospital
Country United Kingdom 
Sector Hospitals 
PI Contribution Attended meetings and discussions. Submitted STFC CLASP proposal in partnership with SCISCI.
Collaborator Contribution Supported collaborative STFC CLASP grant Proposal
Impact Grant proposal submitted.
Start Year 2015
 
Title Mechanical bioreactor 
Description https://patents.google.com/patent/GB201514734D0/en 
IP Reference GB201514734D0 
Protection Patent application published
Year Protection Granted 2017
Licensed No
Impact Development of stem cell derived osteoblasts (bone building cells) for research, drug discovery, and regenerative medicine (surgical bone graft).
 
Description BBC Radio World Service Interview 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interviews throughout 29th December 2017 in relation to the Find A Better Way (FABW) landmine charity, which funded technology that was used to save the leg of a canine patient (Eva the dog).
Year(s) Of Engagement Activity 2017
URL https://www.findabetterway.org.uk/news/extensive-bbc-coverage-synthetic-bone-project/
 
Description BlueDot Music Festival - talk on gravitational waves 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact BlueDot
Year(s) Of Engagement Activity 2016
URL http://www.discoverthebluedot.com
 
Description Careers networking event - Morrisons Academy high school, Crieff, Perthshire 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Presented my research areas (gravitational waves, nanokicking/stem cells) and described career opportunities within physics and biomedical engineering.
Year(s) Of Engagement Activity 2018
URL https://twitter.com/macmorrisons/status/971349384926519296
 
Description Invited public talk at Joint Congress of University Astronomical Societies, Galway, Ireland (Mar 2019) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Invited public talk at Joint Congress of University Astronomical Societies, Galway, Ireland (Mar 2019)
Year(s) Of Engagement Activity 2019
 
Description Invited seminar/colloquim, University of Glasgow (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited talk on Nanokicking (stem cell) research.
Year(s) Of Engagement Activity 2016
 
Description Invited talk to the Renfrewshire Astronomical Society, Paisley 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Invited talk to the Renfrewshire Astronomical Society, Paisley
Year(s) Of Engagement Activity 2017
 
Description Invited talk to the Stirling Astronomical Society, Paisley. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact as above
Year(s) Of Engagement Activity 2017
 
Description Keynote public lecture for the British Orthopaedic Research Society (BORS). 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact BORS
Year(s) Of Engagement Activity 2016
URL http://borsoc.org.uk/
 
Description National coverage on BBC Radio Scotland - Good Morning Scotland show - interview regarding STFC-CLASP project for possible treatment of disuse osteoporosis 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact National coverage on BBC Radio Scotland - Good Morning Scotland show - interview regarding STFC-CLASP project for possible treatment of disuse osteoporosis
Year(s) Of Engagement Activity 2019
 
Description Public talk with Prof. Andrew Hart (Plastic Surgeon), Clinicians in Research Network 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2016
 
Description Radio 4 PM - live interview - Eva the dog story (bone regeneration to save her leg) 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Interviews throughout 29th December 2017 in relation to the Find A Better Way (FABW) landmine charity, which funded technology that was used to save the leg of a canine patient (Eva the dog).
Year(s) Of Engagement Activity 2017
URL https://www.findabetterway.org.uk/news/extensive-bbc-coverage-synthetic-bone-project/
 
Description Re-write the Headlines activity with local school - 1 workshop session - Kingcase Primary school (UK) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Workshop
Year(s) Of Engagement Activity 2015
URL http://researchtheheadlines.org
 
Description Royal Society Summer Science Exhibition - Giving Stem Cells A Good (Nano)kicking 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact 14500 visitors over 7 days.
Year(s) Of Engagement Activity 2016
URL http://www.nanokick.com
 
Description Science in Parliament event - Scottish Government - Society of Chemical Industry (SCI) stand - Reid to lead 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Science and Parliament engagement event, organised by the Royal Society of Chemistry
Year(s) Of Engagement Activity 2015
URL http://www.rsc.org/events/detail/19918/Science%20and%20the%20Parliament%202015
 
Description The Borders Science Festival (Peebles public library) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Public talk on gravitational wave detection at the Borders Science Festival
Year(s) Of Engagement Activity 2017
URL https://www.google.co.uk/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=0ahUKEwjRx-Pp-9...